重力驱动微流控芯片流动注射分析系统的研究
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摘要
本论文建立了一种基于微流控芯片的无泵无阀的重力驱动门式进样流动注射分析系统。系统采用重力(液位差)提供试样注射、试样与试剂混合、传输和反应的驱动力,只需要控制一个液池的移动就能实现芯片上简单有效的门式进样,系统的整体结构和操作得以大大简化。该芯片上还集成加工了液芯波导长光程光度检测系统,以提高系统的检测灵敏度。该系统被应用于亚硝酸盐的吸收光度检测。在优化的实验条件下,采用1秒的进样时间,可获得1.57 nL的进样体积。系统对亚硝酸盐测定的重现性为0.3%(RSD,n=17),线性范围是0.1-20 mg/L,检测限为0.19 mg/L,最高分析通量达113样/小时。该系统具有结构简单、易于操作、集成化程度高、加工成本低、利于普及等优点。
微流控分析系统发展的重要瓶颈之一是宏观体系与芯片微通道的接口问题,开发自动化的试样更换方法,对于微流控分析系统的实用化至关重要。以上工作基础上,本文通过在玻璃芯片上加工出两个一体化取样探针,耦合缺口管自动连续试样引入技术,实现了高效快速的试样注入和试样更换,可用于同时或分别更换试样和试剂。采用色素为模型样品对系统的性能进行了初步考察。系统进样重现性为2.5%(RSD)。该系统的加工方法较为简单,试样试剂消耗少,系统集成度较高,易于实现微型化,有望应用于多试样多指标的分析检测。
In this work,a simple and efficient chip-based FIA system without the need of mechanical pump and valve was developed.The present system supplied a simple operation approach for sample injection in microchip under gated injection mode by changing the vertical position of the waste reservoir.A liquid-core waveguide absorbance detection system was integrated to the microchip to achieve high sensitive detection.The performance of the system was tested in the determination of sodium nitrite based on Saltzman reaction under optimized conditions.A precision of 0.3% (RSD,n=17) was achieved using 10 mg/L[NO_2]~(2-) solution.The sample injection volume for each cycle was 1.57 nL with a sampling time of 1 s.The analysis throughput was 113/h.A linear range of 0.1-20 mg/L for detection of[NO_2]~(2-) was obtained with a limit of detection of 0.19 mg/L(3σ).The present system has the advantages of simple structure,ease of operation and application,high integration and low cost.
World-to-chip interfacing is considered as a major challenge,particularly in high-throughput applications requiring frequent sample change.Based on the previous work,we developed a chip basedμ-FIA system with two on-chip probes coupled with slotted-vial array systems in order to perform simultaneous sample/reagent change.Carmine was used as a model sample to test the performance of the system.A reproducibility of 2.5%(RSD,n=9) was obtained.The preliminary results proved the feasibility of the system in changing the sample and reagent simultaneously,which implied the possibility of the system to be applied in clinical assay with multiple samples and multiple analytes.
微流控分析系统发展的重要瓶颈之一是宏观体系与芯片微通道的接口问题,开发自动化的试样更换方法,对于微流控分析系统的实用化至关重要。以上工作基础上,本文通过在玻璃芯片上加工出两个一体化取样探针,耦合缺口管自动连续试样引入技术,实现了高效快速的试样注入和试样更换,可用于同时或分别更换试样和试剂。采用色素为模型样品对系统的性能进行了初步考察。系统进样重现性为2.5%(RSD)。该系统的加工方法较为简单,试样试剂消耗少,系统集成度较高,易于实现微型化,有望应用于多试样多指标的分析检测。
In this work,a simple and efficient chip-based FIA system without the need of mechanical pump and valve was developed.The present system supplied a simple operation approach for sample injection in microchip under gated injection mode by changing the vertical position of the waste reservoir.A liquid-core waveguide absorbance detection system was integrated to the microchip to achieve high sensitive detection.The performance of the system was tested in the determination of sodium nitrite based on Saltzman reaction under optimized conditions.A precision of 0.3% (RSD,n=17) was achieved using 10 mg/L[NO_2]~(2-) solution.The sample injection volume for each cycle was 1.57 nL with a sampling time of 1 s.The analysis throughput was 113/h.A linear range of 0.1-20 mg/L for detection of[NO_2]~(2-) was obtained with a limit of detection of 0.19 mg/L(3σ).The present system has the advantages of simple structure,ease of operation and application,high integration and low cost.
World-to-chip interfacing is considered as a major challenge,particularly in high-throughput applications requiring frequent sample change.Based on the previous work,we developed a chip basedμ-FIA system with two on-chip probes coupled with slotted-vial array systems in order to perform simultaneous sample/reagent change.Carmine was used as a model sample to test the performance of the system.A reproducibility of 2.5%(RSD,n=9) was obtained.The preliminary results proved the feasibility of the system in changing the sample and reagent simultaneously,which implied the possibility of the system to be applied in clinical assay with multiple samples and multiple analytes.
引文
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