杨梅基因组SSR标记开发和雄株遗传多样性分析
|
摘要
杨梅(Myrica rubra)属于杨梅科(Myricaceae)杨梅属,起源于我国,其种质资源丰富,有2000多年的栽培历史。杨梅营养价值高,是天然的绿色保健食品,市场潜力巨大。本研究基于杨梅全基因组鸟枪测序结果开发了大量SSR标记,为以后杨梅遗传图谱构建及育种提供理论依据,并从分子水平上探讨我国杨梅雄株种质资源的遗传多样性及其育种用途,主要研究结果如下：
1.基于杨梅基因组测序的SSR标记开发
首次对杨梅进行全基因组鸟枪测序及初步分析,共获得9.01Gb序列信息,预测其基因组大小约为323Mb,覆盖深度为26x,其基因组杂合度较高,重复序列含量较低；测序后经拼接获得序列总长度达255Mb,其中包含28,602个SSR位点(重复次数≥5),以二核苷酸重复单元类型为主,所占比例为84.73%,三核苷酸重复单元类型占13.78%,四核苷酸重复单元类型占1.34%,五核苷酸重复单元类型及六核苷酸重复单元类型分别占0.12%,0.04%。我们设计并合成600对SSR引物,其中186对具有多态性,从中选择158对用于分析评价29份杨梅及3份近缘种材料的遗传亲缘关系,发现SSR引物在近缘种青杨梅、矮杨梅及蜡杨梅中具有一定的通用性,分别为91.14%,89.87%与46.84%。采用UPGMA对以上所有样品进行聚类分析,结果表明来源于我国西南地区的青杨梅与矮杨梅亲缘关系较近,而来源于美国的蜡杨梅与其它样品亲缘关系最远且划分为一个独立的分支。
2.杨梅雄株遗传多样性及其育种应用的探索
杨梅一般为雌雄异株植物,栽培品种为雌株,其研究报道较多,而雄株很少受到关注。在本研究中调查统计了139份杨梅雄株花序的颜色与形状特性,同时利用13对多态性SSR标记对其遗传多样性进行分析,并与具有代表性的91份雌株群体做对比研究,结果发现杨梅雄株花序按照色泽差异可分为黄绿色、黄红色与红色三种类型；而按照形状差异可分为棒形、圆柱形与长圆锥形；其中黄红色及长圆锥形类型占绝大部分。杨梅雄株群体等位基因数量,期望杂合度(He)及多态信息含量(PIC)的均值都略低于雌株群体,但两者差异不显著(P>0.05)。同时在杨梅雄株群体中发现了10个稀有等位基因；根据UPGMA聚类结果表明,具有相同地域来源的杨梅雄株及雌株表现为亲缘关系较近；我们分别在两个杨梅品种荸荠与丁岙上发现了单个枝条雄花突变现象,而在东魁品种上出现较多的雄花,这些花粉可用于杨梅杂交育种研究。
3.不同性别类型杨梅花粉特性观测
利用10对多态性SSR引物构建了慈溪杨梅雄株、雌株突变雄枝、雌雄同株三种性别类型杨梅植株的指纹图谱,结果发现雌株突变雄枝类型植株为荸荠品种。三种性别类型杨梅花粉经扫描电镜观测结果表明,花粉大小为(22.90-23.63)×(20.27-22.10)gm,具有直径2.90-3.03μm的萌发孔3个,且无显著性差异；花粉外壁呈颗粒状且分布均匀。使用花粉发芽法与碘-碘化钾染色法对花粉的生活力测定发现,雌雄同株类型花粉生活力为最高；最适宜花粉萌发的培养基为5%蔗糖、0.01%硼酸和1%琼脂。
Chinese bayberry (Myrica rubra) is native to China and has been cultivated for more than2000years with abundant germplasm resources. Limited molecular markers have been developed and applied to evaluate genetic diversity and management of genetic resources. This thesis presented the development of large set of simple sequence repeat (SSR) markers from a genome survey and preliminary genetic diversity of Chinese bayberry androphytes. It will provide a basis for genetic map construction and breeding in the future, the main results were summarized as followings:
1. Development of simple sequence repeat (SSR) markers from a genome survey of Chinese Bayberry
Here we report, for the first time, a genome survey of whole genome shotgun data of Myrica rubra to develop a large number of simple sequence repeat (SSR) markers to analyse the genetic diversity of the common cultivated Chinese bayberry and the relationship with three other Myrica species. The whole genome shotgun survey of Chinese bayberry produced9.01Gb of sequence data, about26x coverage of the estimated genome size of323Mb. The genome sequences were highly heterozygous, but with little duplication. From the initial assembled scaffold covering255Mb sequence data,28,602SSRs (≥5repeats) were identified. Dinucleotide was the most common repeat motif with a frequency of84.73%, followed by13.78%trinucleotide,1.34%tetranucleotide,0.12%pentanucleotide and0.04%hexanucleotide. From600primer pairs,186polymorphic SSRs were developed. Of these,158were used to screen29Chinese bayberry accessions and three other Myrica species:91.14%,89.87%and46.84%SSRs could be used in Myrica adenophora, Myrica nana and Myrica cerifera, respectively. The UPGMA dendrogram tree showed that cultivated Myrica rubra is closely related to Myrica adenophora and Myrica nana, originating in southwest China, and very distantly related to Myrica cerifera, originating in America.
2. Exploring the genetic diversity of Chinese bayberry androphytes and the potential use in cross breeding programs
Chinese bayberry is generally dioecious, the female plants have been cultivated for fruit and studied extensively, but male individuals (androphyte) have gained very little attention in the past. Here we described the morphological variation of the male followers in a collection of139androphyte individuals, and compared their genetic diversity with91cultivars using13polymorphic simple sequence repeats (SSR). The staminate inflorescences can be classified in three colors (yellow-green, yellow-red and red) and three shapes (club, cylindrical and long-conical), Yellow-red color and long-conical shape is more frequent. We found that the number of alleles, expected heterozygosity (He), and polymorphism information content (PIC) were slightly lower in the androphyte population than in cultivars, but there was no significant difference (P>0.05). Ten new alleles were found among androphyte individuals that did not exist in any of the cultivars investigated. The accessions of both cultivars and androphytes originating from the same region were generally clustered together. In Bi-qi and Ding-ao cultivars, we found one individual each bearing male inflorescences which are limited to a branch, while in Dong-kui, a large proportion of shoots can bear male flowers due to growing conditions and retarding regulator spray. The pollens can be used for crossing with other cultivars.
3. Morphology by Scanning Electron Microscope (SEM) and viability of pollens from different sex individuals of Chinese bayberry
Scanning electron microscope observation was carried out with the pollens of Chinese bayberry from androphyte (male plant), female individuals with a mutant branch of staminate inflorescence and monoecious plant. The results showed that the size of pollen were in the range of (22.90-23.63)×(20.27-22.10) μm and and three germinal poles were2.90-3.03μm in diameter, respectively. The outside surface of pollen was even granular ornamented. The size of three sources'pollens showed no marked difference. Study on pollen viability through germination and I-KI staining method showed that the germination percentage of pollen was the highest from monoecious plant. The optimal culture medium is composed of5%sucrose,0.01%boric acid and1%agar.
1.基于杨梅基因组测序的SSR标记开发
首次对杨梅进行全基因组鸟枪测序及初步分析,共获得9.01Gb序列信息,预测其基因组大小约为323Mb,覆盖深度为26x,其基因组杂合度较高,重复序列含量较低；测序后经拼接获得序列总长度达255Mb,其中包含28,602个SSR位点(重复次数≥5),以二核苷酸重复单元类型为主,所占比例为84.73%,三核苷酸重复单元类型占13.78%,四核苷酸重复单元类型占1.34%,五核苷酸重复单元类型及六核苷酸重复单元类型分别占0.12%,0.04%。我们设计并合成600对SSR引物,其中186对具有多态性,从中选择158对用于分析评价29份杨梅及3份近缘种材料的遗传亲缘关系,发现SSR引物在近缘种青杨梅、矮杨梅及蜡杨梅中具有一定的通用性,分别为91.14%,89.87%与46.84%。采用UPGMA对以上所有样品进行聚类分析,结果表明来源于我国西南地区的青杨梅与矮杨梅亲缘关系较近,而来源于美国的蜡杨梅与其它样品亲缘关系最远且划分为一个独立的分支。
2.杨梅雄株遗传多样性及其育种应用的探索
杨梅一般为雌雄异株植物,栽培品种为雌株,其研究报道较多,而雄株很少受到关注。在本研究中调查统计了139份杨梅雄株花序的颜色与形状特性,同时利用13对多态性SSR标记对其遗传多样性进行分析,并与具有代表性的91份雌株群体做对比研究,结果发现杨梅雄株花序按照色泽差异可分为黄绿色、黄红色与红色三种类型；而按照形状差异可分为棒形、圆柱形与长圆锥形；其中黄红色及长圆锥形类型占绝大部分。杨梅雄株群体等位基因数量,期望杂合度(He)及多态信息含量(PIC)的均值都略低于雌株群体,但两者差异不显著(P>0.05)。同时在杨梅雄株群体中发现了10个稀有等位基因；根据UPGMA聚类结果表明,具有相同地域来源的杨梅雄株及雌株表现为亲缘关系较近；我们分别在两个杨梅品种荸荠与丁岙上发现了单个枝条雄花突变现象,而在东魁品种上出现较多的雄花,这些花粉可用于杨梅杂交育种研究。
3.不同性别类型杨梅花粉特性观测
利用10对多态性SSR引物构建了慈溪杨梅雄株、雌株突变雄枝、雌雄同株三种性别类型杨梅植株的指纹图谱,结果发现雌株突变雄枝类型植株为荸荠品种。三种性别类型杨梅花粉经扫描电镜观测结果表明,花粉大小为(22.90-23.63)×(20.27-22.10)gm,具有直径2.90-3.03μm的萌发孔3个,且无显著性差异；花粉外壁呈颗粒状且分布均匀。使用花粉发芽法与碘-碘化钾染色法对花粉的生活力测定发现,雌雄同株类型花粉生活力为最高；最适宜花粉萌发的培养基为5%蔗糖、0.01%硼酸和1%琼脂。
Chinese bayberry (Myrica rubra) is native to China and has been cultivated for more than2000years with abundant germplasm resources. Limited molecular markers have been developed and applied to evaluate genetic diversity and management of genetic resources. This thesis presented the development of large set of simple sequence repeat (SSR) markers from a genome survey and preliminary genetic diversity of Chinese bayberry androphytes. It will provide a basis for genetic map construction and breeding in the future, the main results were summarized as followings:
1. Development of simple sequence repeat (SSR) markers from a genome survey of Chinese Bayberry
Here we report, for the first time, a genome survey of whole genome shotgun data of Myrica rubra to develop a large number of simple sequence repeat (SSR) markers to analyse the genetic diversity of the common cultivated Chinese bayberry and the relationship with three other Myrica species. The whole genome shotgun survey of Chinese bayberry produced9.01Gb of sequence data, about26x coverage of the estimated genome size of323Mb. The genome sequences were highly heterozygous, but with little duplication. From the initial assembled scaffold covering255Mb sequence data,28,602SSRs (≥5repeats) were identified. Dinucleotide was the most common repeat motif with a frequency of84.73%, followed by13.78%trinucleotide,1.34%tetranucleotide,0.12%pentanucleotide and0.04%hexanucleotide. From600primer pairs,186polymorphic SSRs were developed. Of these,158were used to screen29Chinese bayberry accessions and three other Myrica species:91.14%,89.87%and46.84%SSRs could be used in Myrica adenophora, Myrica nana and Myrica cerifera, respectively. The UPGMA dendrogram tree showed that cultivated Myrica rubra is closely related to Myrica adenophora and Myrica nana, originating in southwest China, and very distantly related to Myrica cerifera, originating in America.
2. Exploring the genetic diversity of Chinese bayberry androphytes and the potential use in cross breeding programs
Chinese bayberry is generally dioecious, the female plants have been cultivated for fruit and studied extensively, but male individuals (androphyte) have gained very little attention in the past. Here we described the morphological variation of the male followers in a collection of139androphyte individuals, and compared their genetic diversity with91cultivars using13polymorphic simple sequence repeats (SSR). The staminate inflorescences can be classified in three colors (yellow-green, yellow-red and red) and three shapes (club, cylindrical and long-conical), Yellow-red color and long-conical shape is more frequent. We found that the number of alleles, expected heterozygosity (He), and polymorphism information content (PIC) were slightly lower in the androphyte population than in cultivars, but there was no significant difference (P>0.05). Ten new alleles were found among androphyte individuals that did not exist in any of the cultivars investigated. The accessions of both cultivars and androphytes originating from the same region were generally clustered together. In Bi-qi and Ding-ao cultivars, we found one individual each bearing male inflorescences which are limited to a branch, while in Dong-kui, a large proportion of shoots can bear male flowers due to growing conditions and retarding regulator spray. The pollens can be used for crossing with other cultivars.
3. Morphology by Scanning Electron Microscope (SEM) and viability of pollens from different sex individuals of Chinese bayberry
Scanning electron microscope observation was carried out with the pollens of Chinese bayberry from androphyte (male plant), female individuals with a mutant branch of staminate inflorescence and monoecious plant. The results showed that the size of pollen were in the range of (22.90-23.63)×(20.27-22.10) μm and and three germinal poles were2.90-3.03μm in diameter, respectively. The outside surface of pollen was even granular ornamented. The size of three sources'pollens showed no marked difference. Study on pollen viability through germination and I-KI staining method showed that the germination percentage of pollen was the highest from monoecious plant. The optimal culture medium is composed of5%sucrose,0.01%boric acid and1%agar.
引文
曹珂,王力荣,朱更瑞,方伟超,陈昌文,赵佩.桃果肉颜色性状的SSR标记及其在品种中的验证.果树学报,2010,27(6)：882-886
柴春燕,焦云,徐绍清,房聪玲,胡志刚,高中山.杨梅雄株花序特性与授粉研究.浙江林业科技,2011,31(5)：74-79
陈怀琼,隋春,魏建和.植物SSR引物开发策略简述.分子植物育种,2009,7(4)：845-851
高佳,汤浩茹,董晓莉,罗娅,果树分子遗传图谱研究进展.北方园艺,2007,(10)：44-50
高源,王昆,田路明,曹玉芬,刘凤之.TP-M13-SSR技术及其在苹果种质资源遗传多样性研究中的应用.植物遗传资源学报,2011,12(2)：228-233
蒋建华,章鹤寿.杨梅雄树类型与生产的关系.1993,(1)：51-52
谷晓明,刘宁.矮杨梅不同类型和毛杨梅过氧化物酶同工酶(POX)的遗传分析.贵州师范大学学报(自然科学版),1998,(2)：13-16
郭枢,李三玉.杨梅核型作为分类依据的初步研究.郭枢柑桔研究文集,1994,129-137
韩明丽.梨遗传连锁图谱的初步构建及部分果实性状的QTL定位.杭州：浙江大学硕士学位论文,2010
黄福平.茶树遗传多样性分析与遗传图谱构建.杭州：浙江大学博士学位论文,2005
黄丽芳,闫林,范睿,姚全胜,刘洋,雷新涛.芒果实生资源遗传多样性的SSR分析.热带作物学报,2011,32(10)：1828-1832
李博,冯慧敏,王静毅,童和林,陈友,武耀廷.基于cpSSR分子标记的香蕉种质资源分类.果树学报,2011,28(6)：1012-1018
李国梁,林伯年,沈德绪.杨梅雌雄株同工酶和酚类物质的鉴别.浙江农业大学学报,1995,21(1)：22-26
李三玉.浙江效益农业百科全书·杨梅.北京：中国农业科学技术出版社,2002
李时荣,胡绪枫.中国杨梅属植物分类及种的分布.全国第3次杨梅科研与生产协作会议资料,1994
李晓强,岑秀芬,韦鹏霄,蒙红梅,何新华.杨梅组织培养防污染与防褐化研究.安徽农业科学,2010,38(19)：9965-9967
李兴军,吕均良,李三玉.中国杨梅研究进展.四川农业大学学报,1999,12(2)：224-229
廖娇,黄春辉,辜青青,曲雪艳,徐小彪.猕猴桃EST-SSR引物筛选及通用性分析.果树学报,2011,28(6)：1111-1116
林伯年,徐林娟,贾春蕾.RAPD技术在杨梅属植物分类研究中的应用.园艺学报,1999,26(4)：221-226
刘旭.遗传标记和遗传图谱构建.作物品种资源,1997,(4)：29-32
潘鸿,何新华,李一伟,郭永泽,黄桂香.广西野生杨梅资源遗传多样性的ISSR分析.果树学报,2008,25(3)：353-357
彭镜汲,李育农,吴光林.果树栽培学各论(南方版).北京：农业出版社,1991
戚行江,郑锡良,梁森苗,邱立军,周利秋,李伯钧.不同分布地杨梅雄株花序特性及花粉扫描电镜观察.果树学报,2005,22(2)：175-178
钱剑林,俞文生,王化坤.江浙地区杨梅主要品种的ISSR分析.植物资源与环境学报,2006,15(3)：17-20
钱皆兵,陈子敏,陈俊伟.优质大果杨梅新品系乌紫杨梅的生物学特性及其RAPD鉴定.果树学报,2007,24(1)：64-67
邱英雄,傅承新,孔航辉.杨梅不同品种的ISSR分析.农业生物技术学报,2002,10(4)：343-346
上官凌飞,李晓颖,宁宁,王玉柱,章镇,房经贵.杏EST-SSR标记的开发.园艺学报,2011,38(1)：43-54
宋立琴,张立彬,张吉军,于凤鸣,肖啸.一个与桃成熟期QTL连锁的SSR标记.园艺学报,2011,38(3)：535-540
万怡震,王跃进,张今今,孙马,杨克强,徐炎.多年生果树植物分子遗传图谱.园艺学报,2002,29(增刊)：629-634
王开发,王宪曾.孢粉学概论.北京：北京大学出版社,1983,59-71
王西成,姜淑苓,上官凌飞,曹玉芬,乔玉山,章镇,房经贵.梨EST-SSR标记的开发及其在梨品种遗传多样性分析中的应用评价.中国农业科学,2010,43(24)：5079-5087
王永芳,李伟,智慧,李海权,刁现民.基于谷子测序开发的SSR标记多态性检测.河北农业科学,2010,14(11)：73-76
吴耕民.江南珍果.杭州：浙江农业大学园艺系,1995,1-60
谢小波,求盈盈,戚行江,郑锡良,邱立军,张跃建.杨梅雌、雄种质遗传关系的RAPD和ISSR分析.果树学报,2008,25(2)：198-202
谢小波,求盈盈,戚行江,郑锡良,邱立军,张跃建.浙江杨梅品种遗传差异的RAPD和ISSR分析.浙江农业学报,2008,20(1)：1-5
谢小波,求盈盈,郑锡良,戚行江,邱立军,黄忠平,王涛,梁森苗.杨梅种间杂交及杂种F1的胚培养.果树学报,2009,26(4)：507-510
谢勇.水稻品种遗传差异与间作田间表现关系研究及DArT芯片技术体系构建.中国农业大学学位论文,2003
徐林娟,贾春蕾RAPD技术在杨梅属植物分类研究中的应用.园艺学报,1999,26(4)：221-226
徐云壁,朱立煌.分子数量遗传学.北京：中国农业出版社,1994,81-85
阳志慧,张孝岳,李先信.果树花粉形态研究进展.湖南农业科学,2009,(3)：133-136
杨向晖,吴颖欣,林顺权.6种枇杷属植物花粉形态扫描电镜观察.果树学报,2009,26(4)：572-576
张博,杜生明,黄敏仁.林木遗传图谱研究现状及发展趋势.中国生物工程杂志,2003,23(4)：14-18
张喜焕,刘宁.4种类型矮杨梅花粉形态扫描电镜观察.贵州师范大学学报(自然科学版),2005,23(2)：6-9
朱天富,王荣飞,甘建平.东魁杨梅雌株雄性化与补救效果.浙江柑橘,2004,21(4)：28-29
庄卫东,潘一山.我国杨梅种质资源研究进展.福建林业科技,2001,28(2)：54-57
Ainsworth, C., Rahman, A., Parker, J., Edwards, G., Intersex inflorescences of Rumex acetosa demonstrate that sex determination is unique to each flower. New Phytologist,2005,165:711-720
Akiko, S., Hiroshi, T., Mode of Pollen-Tube Growth in Pistils of Myrica rubra (Myricaceae):A Comparison with Related Families. Annals of Botany,2006, 97:71-77
Aravanopoulos, F.A., Ganopoulos, I.V., Avramidou, E., Fasoula, D.A., Diamantidis, G., Assessing inter- and intra-cultivar variation in Greek Prunus avium by SSR markers. Plant Genetic Resources -C,2010,8(3):242-248
Asghar, S., Chen, L.G., He, X.H., Qin, Y.H., In vitro adventitious shoot formation and organogenesis from embryonic axes of Myrica rubra Sieb. and Zucc. (Red Bayberry). Asian Journal of Plant Sciences,2005,4(4):356-360
Audergon, J.M., Bourguiba, H., Khadari, B., Krichen, L., Trifi-Farah, N., Santoni, S., Grafting versus seed propagated apricot populations:two main gene pools in Tunisia evidenced by SSR markers and model-based Bayesian clustering. Genetica,2010,138(9-10):1023-1032
Bassam, B.J., Caetanoanolles, G., Silver Staining of DNA in Polyacrylamide Gels. Applied biochemistry and Biotechnol,1993,42(2-3):181-188
Batzoglou, S., Jaffe, D.B., Stanley, K., Butler, J., Gnerre, S., Mauceli, E., Berger, B., Mesirov JP, Lander ES. ARACHNE:A whole-genome shotgun assembler. Genome research,2002,12(1):177-189
Baudouin, L., Martinez, R.T., Berger, A., Dollet, M., Characterization of the genetic diversity of the Tall coconut (Cocos nucifera L.) in the Dominican Republic using microsatellite (SSR) markers. Tree Genetics & Genomes,2010, 6(1):73-81
Chaparro, J.X., Werner, D.J., Omalley, D., Sederoff, R.R., Targeted mapping and linkage analysis of morphological isozyme, and RAPD markers in peach. Theoretical and Applied Genetics,1994,87(7):805-815
Chen, K., Xu, C., Zhang, B., Ferguson, I.B., Red Bayberry:Botany and Horticulture. Horticultural Reviews.2004,83-114
Cheng, Y.J., Zhang, R., Zhu, A.D., Wang, X.J., Yu, J., Zhang, H.R., Gao, J.S., Deng, X.X., Development of Juglans Regia SSR Markers by Data Mining of the EST Database. Plant Molecular Biology Reporter,2010,28(4):646-653
Cheng, Y.P., Chien, C.T., Lin, T.P., Population genetics of geographically restricted and widespread species of Myrica (Myricaceae). The Journal of heredity,2000, 91(1):61-66
Cuc, L.M., Mace, E.S., Crouch, J.H., Quang, V.D., Long, T.D., Varshney, R.K., Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea). BMC Plant Biology,2008,8:55
Erickson, D.L., Hamrick, J.L., Genetic and clonal diversity for Myrica cerifera along a spatiotemporal island chronosequence. Heredity,2003,90(1):25-32
Everett, T.H., The New York Botanical Garden illustrated encyclopedia of horticulture. New York Garland Publishing,1981,7:2131-2492
Faure, S., Noyer, J.L., Horry, J.P., Bakry, F., Lanaud, C., Deleon, D.G., A molecular marker-based linkage map of diploid bananas (Musa acuminata). Theoretical and Applied Genetics,1993,87(4):517-526
Feng, C., Chen, M., Xu, C.J., Bai, L., Yin, X.R., Li, X., Allan, A.C., Ferguson, I.B., Chen, K.S., Transcriptomic analysis of Chinese bayberry (Myrica rubra) fruit development and ripening using RNA-Seq. BMC Genomics,2012,13(1):19
Feng, S.P., Li, W.G., Yu, F., Wang, J.Y., Wu, Y.T., Construction of genetic linkage map for rubber tree (Hevea brasiliensis) based on SSR markers. Hereditas, 2010,32(8):857-863
Fraser, L.G., Harvey, C.F., Crowhurst, R.N., De Silva, H.N., EST-derived microsatellites from Actinidia species and their potential for mapping. Theoretical and Applied Genetics,2004,108(6):1010-1016
Gisbert, A.D., Martinez-Calvo, J., Llacer, G., Badenes, M., Romero, C., Development of two loquat Eriobotrya japonica (Thunb.) Lindl. linkage maps based on AFLPs and SSR markers from different Rosaceae species. Molecular Breeding, 2009,23(3):523-538
Goff, S.A., A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science,2005,309(5736):879-879
Gulsen, O., Uzun, A., Canan, I., Seday, U., Canihos, E., A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers. Euphytica, 2010,173(2):265-277
Hampl, V., Pavlicek, A., Flegr, J., Construction and bootstrap analysis of DNA fingerprinting-based phylogenetic trees with the freeware program FreeTree: application to trichomonad parasites. International journal of systematic and evolutionary microbiology,2001,51:731-735
Han, Y., Zheng, D., Vimolmangkang, S., Khan, M.A., Beever, J.E., Korban, S.S., Integration of physical and genetic maps in apple confirms whole-genome and segmental duplications in the apple genome. Journal of Experimental Botany, 2011,62(14):5117-5130
Handa, T., kajiura, I., Isozyme analysis of yamamomo (Myrica rubra Sieb. et Zucc.) cultivars. Japanese Journal of Breeding,1991,41(2):203-209
Iketani, H., Abe, K., Yamamoto, T., Kotobuki, K., Sato, Y., Saito, T., Terai, O., Matsuta, N., Hayashi, T., Mapping of disease-related genes in Japanese pear using a molecular linkage map with RAPD markers. Breeding Science,2001, 51(3):179-184
Celton, J.M., Tustin, D.S., Chagne, D., Gardiner, S.E., Construction of a dense genetic linkage map for apple rootstocks using SSRs developed from Malus ESTs and Pyrus genomic sequences, Tree Genetics & Genomes,2009, 5:93-107
Jaccoud, D., Peng, K., Feinstein, D., Kilian, A., Diversity Arrays:asolid state technology for sequence information independent genotyping. Nucleic Acids Research,2001,29(4):e25
Kapila, R.K., Yadav, R.S., Plaha, P., Rai, K.N., Yadav, O.P., Hash, C.T., Howarth CJ. Genetic diversity among pearl millet maintainers using microsatellite markers. Plant Breeding,2008,127(1):33-37
Kijas, J.M.H., Thomas, M.R., Fowler, J.C.S., Roose ML. Integration of trinucleotide microsatellites into a linkage map of Citrus. Theoretical and Applied Genetics, 1997,94(5):701-706
Lezar, S., Myburg, A.A., Berger, D.K., Wingfield, M.J., Wing-field, B.D., Development and assessment of microar-ray-based DNA fingerprinting in Eucalyptus grandis. Theoretical and Applied Genetics,2004,109:1329-1336
Lindqvist, C., Scheen, A.C., Yoo, M.J., Grey, P., Oppenheimer, D.G., Leebens-Mack, J.H., Soltis, D.E., Soltis, P.S., Albert, V.A., An expressed sequence tag (EST) library from developing fruits of an Hawaiian endemic mint (Stenogyne rugosa, Lamiaceae):characterization and microsatellite markers. BMC Plant Biology, 2006,6(16):1471-2229
Liu, K.J., Muse, S.V., PowerMarker:an integrated analysis environment for genetic marker analysis. Bioinformatics,2005,21(9):2128-2129
Lu, J., Huang, H., Ren, Z.B., Hunter, W., Dowd, S.E., Dang, P., Mining and validating grape (Vitis L.) ESTs to develop EST-SSR markers for genotyping and mapping. Molecular Breeding,2011,28(2):241-254
Maliepaard, C., Alston, F.H., van Arkel, G., Brown, L.M., Chevreau, E., Dunemann, F., Evans, K.M., Gardiner, S., Guilford, P., van Heusden, A.W., Janse, J., Laurens, F., Lynn, J.R., Manganaris, A.G., den Nijs, A.P.M., Periam, N., Rikkerink, E., Roche, P., Ryder, C., Sansavini, S., Schmidt, H., Tartarini, S., Verhaegh, J.J., Vrielink-van Ginkel, M., King, G.J., Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers. Theoretical and Applied Genetics,1998,97(1-2):60-73
McCouch, S.R., Cho, Y.G., Ishii, T., Temnykh, S., Chen, X., Lipovich, L., Park, W.D., Ayres, N., Cartinhour, S., Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.). Theoretical and Applied Genetics,2000,100(5):713-722
Myers, R.M., Maniatis, T., Lerman, L.S., Detection and Localization of Single Base Changes by Denaturing Gradient Gel-Electrophoresis. Method Enzymology, 1987,155:501-527
Nei, M., Genetic Distance between Populations. American Naturalist,1972, 106(949):283-292
Nishio, S., Yamamoto, T., Terakami, S., Sawamura, Y., Takada, N., Saito, T., Genetic diversity of Japanese chestnut cultivars assessed by SSR markers. Breeding Science,2011,61:109-120
Niu, S.S., Xu, C.J., Zhang, W.S., Zhang, B., Li, X., Lin-Wang, K., Ferguson, I.B. Allan, A.C., Chen, K.S., Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor. Planta,2010,231:887-899
Parasnis, A.S., Ramakrishna, W., Chowdari, K.V., Gupta, V.S., Ranjekar, P.K. Microsatellite (GATA)n reveals sex-specific differences in Papaya. Theoretical and Applied Genetics,1999,99:1047-1052
Parida, S., Kalia, S., Kaul, S., Dalal, V., Hemaprabha, G., Selvi, A., Pandit, A., Singh, A., Gaikwad, K., Sharma, T., Srivastava, P., Singh, N., Mohapatra, T. Informative genomic microsatellite markers for efficient genotyping applications in sugarcane. Theoretical and Applied Genetics,2009,118: 327-338
Parker, J.S., Sex chromosomes and sexual differentiation in flowering plants. Chromosomes Today,1990,10:187-198
Powell, W., Machray, G.C., Provan, J., Polymorphism revealed by simple sequence repeats.Trends in Plant Science,1996,1:215-222
Schuelke, M., An economic method for the fluorescent labeling of PCR fragments. Nature biotechnology,2000,18(2):233-234
Sosinski, B., Gannavarapu, M., Hager, L.D., Beck, L.E., King, G.J., Ryder, C.D., Rajapakse, S., Baird, W.V., Ballard, R.E., Abbott, A.G., Characterization of microsatellite markers in peach [Prunus persica (L.) Batsch]. Theoretical and Applied Genetics,2000,101(3):421-428
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S., MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution,2011,28(10):2731-2739
Tavassolian, I., Rabiei, G., Gregory, D., Mnejja, M., Wirthensohn, M.G., Hunt, P.W., Gibson, J.P., Ford, C.M., Sedgley, M., Wu, S.B., Construction of an almond linkage map in an Australian population Nonpareil x Lauranne. BMC Genomics,2010,11:551
Terakawa, M., Kikuchi, S., Kanetani, S., Matsui, K., Yumoto, T., Yoshimaru, H., Characterization of 13 polymorphic microsatellite loci for an evergreen tree, Myrica rubra. Molecular Ecology Notes,2006,6(3):709-711
Testolin, R., Huang, W.G., Cipriani, G., Towards a linkage map in kiwifruit (Actinidia chinensis Planch.) based on microsatellites and saturated with AFLP markers. Acta Horticulturae,1999,498:79-84
Testolin, R., Huang, W.G., Lain, O., Messina, R., Vecchione, A., Cipriani, G., A kiwifruit (Actinidia spp.) linkage map based on microsatellites and integrated with AFLP markers. Theoretical and Applied Genetics,2001,103(1):30-36
Wang, J., Chen, C., Na, J.K., Yu, Q., Hou, S., Paull, R., Moore, P., Alam, M., Ming, R., Genome-Wide Comparative Analyses of Microsatellites in Papaya. Tropical Plant Biology,2008, 1(3):278-292
Wang, X.Y., Li, J.R., Joyce, D.C., Yang, X.H., Injury of red bayberry during storage and transportation. Food Technology and Quality Evaluation,2003,235-241
Watanabe, K.N., Hirano, R., Ishii, H., Oo, T.H., Gilani, S.A., Kikuchi, A., Propagation management methods have altered the genetic variability of two traditional mango varieties in Myanmar, as revealed by SSR. Plant Genet Resour-C,2011,9(3):404-410
Wenzl, P., Carling, J., Kudrna, D., Jaccoud, D., Huttner, E., Kleinhofs, A., Kilian, A., Diversity arrays technology (DArT) for whole-genome profiling of barley. Proceedings of the National Academy of Sciences,2004,101 (26):9915-9920
Wenzl, P., Raman, H., Wang, J., Zhou, M., Huttner, D., Kilian, A., A DArT platform for quantitative bulked segregant analysis. BMC Genomics,2007,8:196
Wittenberg, A.H.J., Lee, T.V.D., Cayla, C., Kilian, A., Visser, R.G.F., Schouten HJ. Validation of the high-throughput marker technology DArT using the model plant Arabidop-sis thaliana. Molecular Genet and Genomics,2005,274:30-39
Wu, Y.Q., Wang, Y.W., Samuels, T.D., Development of 1,030 genomic SSR markers in switchgrass. Theoretical and Applied Genetics,2011,122(4):677-686
Xie, R.J., Zhou, J., Wang, G.Y., Zhang, S.M., Chen, L., Gao, Z.S., Cultivar Identification and Genetic Diversity of Chinese Bayberry (Myrica rubra) Accessions Based on Fluorescent SSR Markers. Plant Molecular Biology Reporter,2011,29(3):554-562
Xie, X.B., Qiu, Y.Y., Ke, L.P., Zheng, X.L., Wu, G.T., Chen, J.Q., Qi, X.J., Ahn, S., Microsatellite Primers in Red Bayberry, Myrica Rubra (Myricaceae). American Journal of Botany,2011,98(4):E93-E95
Yamamoto, T., Kimura, T., Saito, T., Kotobuki, K., Matsuta, N., Liebhard, R., Gessler, C., Weg, W.E., Van De, Hayashi, T., Genetic linkage maps of Japanese and European pears aligned to the apple consensus map. Acta Horticulturae,2004, 663
Yeh, F.C., Yang, R.C., Boyle, T., POPGENE (Version 1.31):Microsoft Window-bases freeware for population genetic analysis, University of Alberta and the Centre for International Forestry Research,1999
Yen, T.K., Structure and development of the flower and the fruit of Myrica rubra Sieb. & Zucc. Peking Natural History Bulletin,1950,19:1-20
Zhang, B., Kang, M.X., Xie, Q.P., Xu, B., Sun, C.D., Chen, K.S., Wu, Y.L Anthocyanins from Chinese Bayberry Extract Protect beta Cells from Oxidative Stress-Mediated Injury via HO-1 Upregulation. Journal of Agricultural and Food Chemistry,2011,59(2):537-545
Zhang, S.M., Gao, Z.S., Xu, C.J., Chen, K.S., Genetic Diversity of Chinese Bayberry (Myrica rubra Sieb.& Zucc.) Accessions Revealed by Amplified Fragment Length Polymorphism. Hortscience,2009a,44(2):487-491
Zhang, S.M., Xu, C.J., Gao, Z.S., Chen, K.S., Wang, G.Y., Development and characterization of microsatellite markers for Chinese bayberry (Myrica rubra Sieb.& Zucc.). Conserv Genet,2009b,10(5):1605-1607
柴春燕,焦云,徐绍清,房聪玲,胡志刚,高中山.杨梅雄株花序特性与授粉研究.浙江林业科技,2011,31(5)：74-79
陈怀琼,隋春,魏建和.植物SSR引物开发策略简述.分子植物育种,2009,7(4)：845-851
高佳,汤浩茹,董晓莉,罗娅,果树分子遗传图谱研究进展.北方园艺,2007,(10)：44-50
高源,王昆,田路明,曹玉芬,刘凤之.TP-M13-SSR技术及其在苹果种质资源遗传多样性研究中的应用.植物遗传资源学报,2011,12(2)：228-233
蒋建华,章鹤寿.杨梅雄树类型与生产的关系.1993,(1)：51-52
谷晓明,刘宁.矮杨梅不同类型和毛杨梅过氧化物酶同工酶(POX)的遗传分析.贵州师范大学学报(自然科学版),1998,(2)：13-16
郭枢,李三玉.杨梅核型作为分类依据的初步研究.郭枢柑桔研究文集,1994,129-137
韩明丽.梨遗传连锁图谱的初步构建及部分果实性状的QTL定位.杭州：浙江大学硕士学位论文,2010
黄福平.茶树遗传多样性分析与遗传图谱构建.杭州：浙江大学博士学位论文,2005
黄丽芳,闫林,范睿,姚全胜,刘洋,雷新涛.芒果实生资源遗传多样性的SSR分析.热带作物学报,2011,32(10)：1828-1832
李博,冯慧敏,王静毅,童和林,陈友,武耀廷.基于cpSSR分子标记的香蕉种质资源分类.果树学报,2011,28(6)：1012-1018
李国梁,林伯年,沈德绪.杨梅雌雄株同工酶和酚类物质的鉴别.浙江农业大学学报,1995,21(1)：22-26
李三玉.浙江效益农业百科全书·杨梅.北京：中国农业科学技术出版社,2002
李时荣,胡绪枫.中国杨梅属植物分类及种的分布.全国第3次杨梅科研与生产协作会议资料,1994
李晓强,岑秀芬,韦鹏霄,蒙红梅,何新华.杨梅组织培养防污染与防褐化研究.安徽农业科学,2010,38(19)：9965-9967
李兴军,吕均良,李三玉.中国杨梅研究进展.四川农业大学学报,1999,12(2)：224-229
廖娇,黄春辉,辜青青,曲雪艳,徐小彪.猕猴桃EST-SSR引物筛选及通用性分析.果树学报,2011,28(6)：1111-1116
林伯年,徐林娟,贾春蕾.RAPD技术在杨梅属植物分类研究中的应用.园艺学报,1999,26(4)：221-226
刘旭.遗传标记和遗传图谱构建.作物品种资源,1997,(4)：29-32
潘鸿,何新华,李一伟,郭永泽,黄桂香.广西野生杨梅资源遗传多样性的ISSR分析.果树学报,2008,25(3)：353-357
彭镜汲,李育农,吴光林.果树栽培学各论(南方版).北京：农业出版社,1991
戚行江,郑锡良,梁森苗,邱立军,周利秋,李伯钧.不同分布地杨梅雄株花序特性及花粉扫描电镜观察.果树学报,2005,22(2)：175-178
钱剑林,俞文生,王化坤.江浙地区杨梅主要品种的ISSR分析.植物资源与环境学报,2006,15(3)：17-20
钱皆兵,陈子敏,陈俊伟.优质大果杨梅新品系乌紫杨梅的生物学特性及其RAPD鉴定.果树学报,2007,24(1)：64-67
邱英雄,傅承新,孔航辉.杨梅不同品种的ISSR分析.农业生物技术学报,2002,10(4)：343-346
上官凌飞,李晓颖,宁宁,王玉柱,章镇,房经贵.杏EST-SSR标记的开发.园艺学报,2011,38(1)：43-54
宋立琴,张立彬,张吉军,于凤鸣,肖啸.一个与桃成熟期QTL连锁的SSR标记.园艺学报,2011,38(3)：535-540
万怡震,王跃进,张今今,孙马,杨克强,徐炎.多年生果树植物分子遗传图谱.园艺学报,2002,29(增刊)：629-634
王开发,王宪曾.孢粉学概论.北京：北京大学出版社,1983,59-71
王西成,姜淑苓,上官凌飞,曹玉芬,乔玉山,章镇,房经贵.梨EST-SSR标记的开发及其在梨品种遗传多样性分析中的应用评价.中国农业科学,2010,43(24)：5079-5087
王永芳,李伟,智慧,李海权,刁现民.基于谷子测序开发的SSR标记多态性检测.河北农业科学,2010,14(11)：73-76
吴耕民.江南珍果.杭州：浙江农业大学园艺系,1995,1-60
谢小波,求盈盈,戚行江,郑锡良,邱立军,张跃建.杨梅雌、雄种质遗传关系的RAPD和ISSR分析.果树学报,2008,25(2)：198-202
谢小波,求盈盈,戚行江,郑锡良,邱立军,张跃建.浙江杨梅品种遗传差异的RAPD和ISSR分析.浙江农业学报,2008,20(1)：1-5
谢小波,求盈盈,郑锡良,戚行江,邱立军,黄忠平,王涛,梁森苗.杨梅种间杂交及杂种F1的胚培养.果树学报,2009,26(4)：507-510
谢勇.水稻品种遗传差异与间作田间表现关系研究及DArT芯片技术体系构建.中国农业大学学位论文,2003
徐林娟,贾春蕾RAPD技术在杨梅属植物分类研究中的应用.园艺学报,1999,26(4)：221-226
徐云壁,朱立煌.分子数量遗传学.北京：中国农业出版社,1994,81-85
阳志慧,张孝岳,李先信.果树花粉形态研究进展.湖南农业科学,2009,(3)：133-136
杨向晖,吴颖欣,林顺权.6种枇杷属植物花粉形态扫描电镜观察.果树学报,2009,26(4)：572-576
张博,杜生明,黄敏仁.林木遗传图谱研究现状及发展趋势.中国生物工程杂志,2003,23(4)：14-18
张喜焕,刘宁.4种类型矮杨梅花粉形态扫描电镜观察.贵州师范大学学报(自然科学版),2005,23(2)：6-9
朱天富,王荣飞,甘建平.东魁杨梅雌株雄性化与补救效果.浙江柑橘,2004,21(4)：28-29
庄卫东,潘一山.我国杨梅种质资源研究进展.福建林业科技,2001,28(2)：54-57
Ainsworth, C., Rahman, A., Parker, J., Edwards, G., Intersex inflorescences of Rumex acetosa demonstrate that sex determination is unique to each flower. New Phytologist,2005,165:711-720
Akiko, S., Hiroshi, T., Mode of Pollen-Tube Growth in Pistils of Myrica rubra (Myricaceae):A Comparison with Related Families. Annals of Botany,2006, 97:71-77
Aravanopoulos, F.A., Ganopoulos, I.V., Avramidou, E., Fasoula, D.A., Diamantidis, G., Assessing inter- and intra-cultivar variation in Greek Prunus avium by SSR markers. Plant Genetic Resources -C,2010,8(3):242-248
Asghar, S., Chen, L.G., He, X.H., Qin, Y.H., In vitro adventitious shoot formation and organogenesis from embryonic axes of Myrica rubra Sieb. and Zucc. (Red Bayberry). Asian Journal of Plant Sciences,2005,4(4):356-360
Audergon, J.M., Bourguiba, H., Khadari, B., Krichen, L., Trifi-Farah, N., Santoni, S., Grafting versus seed propagated apricot populations:two main gene pools in Tunisia evidenced by SSR markers and model-based Bayesian clustering. Genetica,2010,138(9-10):1023-1032
Bassam, B.J., Caetanoanolles, G., Silver Staining of DNA in Polyacrylamide Gels. Applied biochemistry and Biotechnol,1993,42(2-3):181-188
Batzoglou, S., Jaffe, D.B., Stanley, K., Butler, J., Gnerre, S., Mauceli, E., Berger, B., Mesirov JP, Lander ES. ARACHNE:A whole-genome shotgun assembler. Genome research,2002,12(1):177-189
Baudouin, L., Martinez, R.T., Berger, A., Dollet, M., Characterization of the genetic diversity of the Tall coconut (Cocos nucifera L.) in the Dominican Republic using microsatellite (SSR) markers. Tree Genetics & Genomes,2010, 6(1):73-81
Chaparro, J.X., Werner, D.J., Omalley, D., Sederoff, R.R., Targeted mapping and linkage analysis of morphological isozyme, and RAPD markers in peach. Theoretical and Applied Genetics,1994,87(7):805-815
Chen, K., Xu, C., Zhang, B., Ferguson, I.B., Red Bayberry:Botany and Horticulture. Horticultural Reviews.2004,83-114
Cheng, Y.J., Zhang, R., Zhu, A.D., Wang, X.J., Yu, J., Zhang, H.R., Gao, J.S., Deng, X.X., Development of Juglans Regia SSR Markers by Data Mining of the EST Database. Plant Molecular Biology Reporter,2010,28(4):646-653
Cheng, Y.P., Chien, C.T., Lin, T.P., Population genetics of geographically restricted and widespread species of Myrica (Myricaceae). The Journal of heredity,2000, 91(1):61-66
Cuc, L.M., Mace, E.S., Crouch, J.H., Quang, V.D., Long, T.D., Varshney, R.K., Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea). BMC Plant Biology,2008,8:55
Erickson, D.L., Hamrick, J.L., Genetic and clonal diversity for Myrica cerifera along a spatiotemporal island chronosequence. Heredity,2003,90(1):25-32
Everett, T.H., The New York Botanical Garden illustrated encyclopedia of horticulture. New York Garland Publishing,1981,7:2131-2492
Faure, S., Noyer, J.L., Horry, J.P., Bakry, F., Lanaud, C., Deleon, D.G., A molecular marker-based linkage map of diploid bananas (Musa acuminata). Theoretical and Applied Genetics,1993,87(4):517-526
Feng, C., Chen, M., Xu, C.J., Bai, L., Yin, X.R., Li, X., Allan, A.C., Ferguson, I.B., Chen, K.S., Transcriptomic analysis of Chinese bayberry (Myrica rubra) fruit development and ripening using RNA-Seq. BMC Genomics,2012,13(1):19
Feng, S.P., Li, W.G., Yu, F., Wang, J.Y., Wu, Y.T., Construction of genetic linkage map for rubber tree (Hevea brasiliensis) based on SSR markers. Hereditas, 2010,32(8):857-863
Fraser, L.G., Harvey, C.F., Crowhurst, R.N., De Silva, H.N., EST-derived microsatellites from Actinidia species and their potential for mapping. Theoretical and Applied Genetics,2004,108(6):1010-1016
Gisbert, A.D., Martinez-Calvo, J., Llacer, G., Badenes, M., Romero, C., Development of two loquat Eriobotrya japonica (Thunb.) Lindl. linkage maps based on AFLPs and SSR markers from different Rosaceae species. Molecular Breeding, 2009,23(3):523-538
Goff, S.A., A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science,2005,309(5736):879-879
Gulsen, O., Uzun, A., Canan, I., Seday, U., Canihos, E., A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers. Euphytica, 2010,173(2):265-277
Hampl, V., Pavlicek, A., Flegr, J., Construction and bootstrap analysis of DNA fingerprinting-based phylogenetic trees with the freeware program FreeTree: application to trichomonad parasites. International journal of systematic and evolutionary microbiology,2001,51:731-735
Han, Y., Zheng, D., Vimolmangkang, S., Khan, M.A., Beever, J.E., Korban, S.S., Integration of physical and genetic maps in apple confirms whole-genome and segmental duplications in the apple genome. Journal of Experimental Botany, 2011,62(14):5117-5130
Handa, T., kajiura, I., Isozyme analysis of yamamomo (Myrica rubra Sieb. et Zucc.) cultivars. Japanese Journal of Breeding,1991,41(2):203-209
Iketani, H., Abe, K., Yamamoto, T., Kotobuki, K., Sato, Y., Saito, T., Terai, O., Matsuta, N., Hayashi, T., Mapping of disease-related genes in Japanese pear using a molecular linkage map with RAPD markers. Breeding Science,2001, 51(3):179-184
Celton, J.M., Tustin, D.S., Chagne, D., Gardiner, S.E., Construction of a dense genetic linkage map for apple rootstocks using SSRs developed from Malus ESTs and Pyrus genomic sequences, Tree Genetics & Genomes,2009, 5:93-107
Jaccoud, D., Peng, K., Feinstein, D., Kilian, A., Diversity Arrays:asolid state technology for sequence information independent genotyping. Nucleic Acids Research,2001,29(4):e25
Kapila, R.K., Yadav, R.S., Plaha, P., Rai, K.N., Yadav, O.P., Hash, C.T., Howarth CJ. Genetic diversity among pearl millet maintainers using microsatellite markers. Plant Breeding,2008,127(1):33-37
Kijas, J.M.H., Thomas, M.R., Fowler, J.C.S., Roose ML. Integration of trinucleotide microsatellites into a linkage map of Citrus. Theoretical and Applied Genetics, 1997,94(5):701-706
Lezar, S., Myburg, A.A., Berger, D.K., Wingfield, M.J., Wing-field, B.D., Development and assessment of microar-ray-based DNA fingerprinting in Eucalyptus grandis. Theoretical and Applied Genetics,2004,109:1329-1336
Lindqvist, C., Scheen, A.C., Yoo, M.J., Grey, P., Oppenheimer, D.G., Leebens-Mack, J.H., Soltis, D.E., Soltis, P.S., Albert, V.A., An expressed sequence tag (EST) library from developing fruits of an Hawaiian endemic mint (Stenogyne rugosa, Lamiaceae):characterization and microsatellite markers. BMC Plant Biology, 2006,6(16):1471-2229
Liu, K.J., Muse, S.V., PowerMarker:an integrated analysis environment for genetic marker analysis. Bioinformatics,2005,21(9):2128-2129
Lu, J., Huang, H., Ren, Z.B., Hunter, W., Dowd, S.E., Dang, P., Mining and validating grape (Vitis L.) ESTs to develop EST-SSR markers for genotyping and mapping. Molecular Breeding,2011,28(2):241-254
Maliepaard, C., Alston, F.H., van Arkel, G., Brown, L.M., Chevreau, E., Dunemann, F., Evans, K.M., Gardiner, S., Guilford, P., van Heusden, A.W., Janse, J., Laurens, F., Lynn, J.R., Manganaris, A.G., den Nijs, A.P.M., Periam, N., Rikkerink, E., Roche, P., Ryder, C., Sansavini, S., Schmidt, H., Tartarini, S., Verhaegh, J.J., Vrielink-van Ginkel, M., King, G.J., Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers. Theoretical and Applied Genetics,1998,97(1-2):60-73
McCouch, S.R., Cho, Y.G., Ishii, T., Temnykh, S., Chen, X., Lipovich, L., Park, W.D., Ayres, N., Cartinhour, S., Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.). Theoretical and Applied Genetics,2000,100(5):713-722
Myers, R.M., Maniatis, T., Lerman, L.S., Detection and Localization of Single Base Changes by Denaturing Gradient Gel-Electrophoresis. Method Enzymology, 1987,155:501-527
Nei, M., Genetic Distance between Populations. American Naturalist,1972, 106(949):283-292
Nishio, S., Yamamoto, T., Terakami, S., Sawamura, Y., Takada, N., Saito, T., Genetic diversity of Japanese chestnut cultivars assessed by SSR markers. Breeding Science,2011,61:109-120
Niu, S.S., Xu, C.J., Zhang, W.S., Zhang, B., Li, X., Lin-Wang, K., Ferguson, I.B. Allan, A.C., Chen, K.S., Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor. Planta,2010,231:887-899
Parasnis, A.S., Ramakrishna, W., Chowdari, K.V., Gupta, V.S., Ranjekar, P.K. Microsatellite (GATA)n reveals sex-specific differences in Papaya. Theoretical and Applied Genetics,1999,99:1047-1052
Parida, S., Kalia, S., Kaul, S., Dalal, V., Hemaprabha, G., Selvi, A., Pandit, A., Singh, A., Gaikwad, K., Sharma, T., Srivastava, P., Singh, N., Mohapatra, T. Informative genomic microsatellite markers for efficient genotyping applications in sugarcane. Theoretical and Applied Genetics,2009,118: 327-338
Parker, J.S., Sex chromosomes and sexual differentiation in flowering plants. Chromosomes Today,1990,10:187-198
Powell, W., Machray, G.C., Provan, J., Polymorphism revealed by simple sequence repeats.Trends in Plant Science,1996,1:215-222
Schuelke, M., An economic method for the fluorescent labeling of PCR fragments. Nature biotechnology,2000,18(2):233-234
Sosinski, B., Gannavarapu, M., Hager, L.D., Beck, L.E., King, G.J., Ryder, C.D., Rajapakse, S., Baird, W.V., Ballard, R.E., Abbott, A.G., Characterization of microsatellite markers in peach [Prunus persica (L.) Batsch]. Theoretical and Applied Genetics,2000,101(3):421-428
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S., MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution,2011,28(10):2731-2739
Tavassolian, I., Rabiei, G., Gregory, D., Mnejja, M., Wirthensohn, M.G., Hunt, P.W., Gibson, J.P., Ford, C.M., Sedgley, M., Wu, S.B., Construction of an almond linkage map in an Australian population Nonpareil x Lauranne. BMC Genomics,2010,11:551
Terakawa, M., Kikuchi, S., Kanetani, S., Matsui, K., Yumoto, T., Yoshimaru, H., Characterization of 13 polymorphic microsatellite loci for an evergreen tree, Myrica rubra. Molecular Ecology Notes,2006,6(3):709-711
Testolin, R., Huang, W.G., Cipriani, G., Towards a linkage map in kiwifruit (Actinidia chinensis Planch.) based on microsatellites and saturated with AFLP markers. Acta Horticulturae,1999,498:79-84
Testolin, R., Huang, W.G., Lain, O., Messina, R., Vecchione, A., Cipriani, G., A kiwifruit (Actinidia spp.) linkage map based on microsatellites and integrated with AFLP markers. Theoretical and Applied Genetics,2001,103(1):30-36
Wang, J., Chen, C., Na, J.K., Yu, Q., Hou, S., Paull, R., Moore, P., Alam, M., Ming, R., Genome-Wide Comparative Analyses of Microsatellites in Papaya. Tropical Plant Biology,2008, 1(3):278-292
Wang, X.Y., Li, J.R., Joyce, D.C., Yang, X.H., Injury of red bayberry during storage and transportation. Food Technology and Quality Evaluation,2003,235-241
Watanabe, K.N., Hirano, R., Ishii, H., Oo, T.H., Gilani, S.A., Kikuchi, A., Propagation management methods have altered the genetic variability of two traditional mango varieties in Myanmar, as revealed by SSR. Plant Genet Resour-C,2011,9(3):404-410
Wenzl, P., Carling, J., Kudrna, D., Jaccoud, D., Huttner, E., Kleinhofs, A., Kilian, A., Diversity arrays technology (DArT) for whole-genome profiling of barley. Proceedings of the National Academy of Sciences,2004,101 (26):9915-9920
Wenzl, P., Raman, H., Wang, J., Zhou, M., Huttner, D., Kilian, A., A DArT platform for quantitative bulked segregant analysis. BMC Genomics,2007,8:196
Wittenberg, A.H.J., Lee, T.V.D., Cayla, C., Kilian, A., Visser, R.G.F., Schouten HJ. Validation of the high-throughput marker technology DArT using the model plant Arabidop-sis thaliana. Molecular Genet and Genomics,2005,274:30-39
Wu, Y.Q., Wang, Y.W., Samuels, T.D., Development of 1,030 genomic SSR markers in switchgrass. Theoretical and Applied Genetics,2011,122(4):677-686
Xie, R.J., Zhou, J., Wang, G.Y., Zhang, S.M., Chen, L., Gao, Z.S., Cultivar Identification and Genetic Diversity of Chinese Bayberry (Myrica rubra) Accessions Based on Fluorescent SSR Markers. Plant Molecular Biology Reporter,2011,29(3):554-562
Xie, X.B., Qiu, Y.Y., Ke, L.P., Zheng, X.L., Wu, G.T., Chen, J.Q., Qi, X.J., Ahn, S., Microsatellite Primers in Red Bayberry, Myrica Rubra (Myricaceae). American Journal of Botany,2011,98(4):E93-E95
Yamamoto, T., Kimura, T., Saito, T., Kotobuki, K., Matsuta, N., Liebhard, R., Gessler, C., Weg, W.E., Van De, Hayashi, T., Genetic linkage maps of Japanese and European pears aligned to the apple consensus map. Acta Horticulturae,2004, 663
Yeh, F.C., Yang, R.C., Boyle, T., POPGENE (Version 1.31):Microsoft Window-bases freeware for population genetic analysis, University of Alberta and the Centre for International Forestry Research,1999
Yen, T.K., Structure and development of the flower and the fruit of Myrica rubra Sieb. & Zucc. Peking Natural History Bulletin,1950,19:1-20
Zhang, B., Kang, M.X., Xie, Q.P., Xu, B., Sun, C.D., Chen, K.S., Wu, Y.L Anthocyanins from Chinese Bayberry Extract Protect beta Cells from Oxidative Stress-Mediated Injury via HO-1 Upregulation. Journal of Agricultural and Food Chemistry,2011,59(2):537-545
Zhang, S.M., Gao, Z.S., Xu, C.J., Chen, K.S., Genetic Diversity of Chinese Bayberry (Myrica rubra Sieb.& Zucc.) Accessions Revealed by Amplified Fragment Length Polymorphism. Hortscience,2009a,44(2):487-491
Zhang, S.M., Xu, C.J., Gao, Z.S., Chen, K.S., Wang, G.Y., Development and characterization of microsatellite markers for Chinese bayberry (Myrica rubra Sieb.& Zucc.). Conserv Genet,2009b,10(5):1605-1607