川芎及其主要成分和达乌尔黄鼠冬眠期抗废用性肌萎缩的机制研究
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摘要
研究目的:
1.对川芎及川芎中起活血作用的两种主要药效成分(阿魏酸钠和川芎嗪)对后肢去负荷大鼠比目鱼肌萎缩的影响与作用机制进行初步研究。
2.对达乌尔黄鼠冬眠期抗废用性肌萎缩的可能机制进行初步探讨。
研究方法:
1.尾部悬吊法建立大鼠废用性肌萎缩模型,利用免疫组织化学、血液流变学和梭内外肌纤维超微结构三个方面的研究方法观察药物对比目鱼肌的影响。
2.利用血液粘度计和电子显微镜观察并研究达乌尔黄鼠血流变特性及冬眠对梭内外肌纤维超微结构的影响。
研究结果:
1.与后肢去负荷大鼠相比1)高剂量的阿魏酸钠和川芎嗪使比目鱼肌Ⅰ型肌纤维横截面积分别增加了37.3%和39.4%(P＜0.05);2)三种药物均能明显抑制梭外肌纤维MHCⅡ表达水平的升高(P＜0.01);3)使肌梭内核袋2纤维MHC的表达由阳性转变为阴性;4)能明显降低低切变率下的全血粘度;5)对梭内外肌纤维超微结构的改变有明显的抑制作用。
2.1)冬眠中黄鼠全血粘度较活跃期升高,出眠时下降;2)尾部悬吊对黄鼠的血流变特性无明显改变:3)梭内外肌纤维超微结构在冬眠中无较大改变,出眠时较冬眠中加重。
研究结论:
1.川芎及其两种主要药效成分阿魏酸钠与川芎嗪均能不同程度地对抗废用性肌萎缩的发生,以高剂量的阿魏酸钠和川芎嗪药效最为明显。
2.黄鼠血流变特性和梭内外肌纤维超微结构的改变与其生理特性相关,这可能也是其冬眠过程中抗肌萎缩的原因之一。
Objective:
1. To study the effects and their mechanism of Ligusticum chuanxiong and its two components (Sodium Ferulate and Ligustrazine as main efficacy components in Ligusticum chuanxiong for invigorating blood circulation) on muscle atrophy in a hind limb unloaded rat model.
2. To study the ground squirrel's possible mechanisms of anti-muscle atrophy during hibernation.
Methods:
1. The tail-suspended rats were subjected to a 14-days disuse, immunohistochemic, hemorheology property and muscle fiber ultrastructure in/out muscle spindle were used to study the effects of medicines on soleus muscle.
2. Blood viscometer and Electron microscope were used to investigate the ground squirrel's hemorheology property and muscle fiber ultrastructure in/out muscle spindle during hibernation.
Results:
1. Compared with HLU+W, 1) the CSA of soleus type I fibers in HLU+SfH and HLU+TmpH increased by 37.3% and 39.4% respectively (P<0.05); 2) expression level of MHCII were inhibited in all treatment groups (P<0.01); 3) expression of MHC in nuclear bag 2 fiber were altered from positive to negative; 4) the blood viscosity in low shear rates decreased obviously (P<0.001), even near to CON's; 5) and show the inhibited effect on change of muscle fiber ultrastructure in/out muscle spindle.
2. 1) Compare with Aut-A, whole blood viscosity increase during hibernation and decrease after hibernation. 2) Hemorheology property have no significant change after hindlimb-unloading. 3) Muscle fiber ultrastructure in/out muscle spindle have no significant change during hibernation, and after hibernation it worse than H-m.
Conclusion:
1. Ligusticum chuanxiong and its two main efficacy components (Sodium Ferulate and Ligustrazine) can prevent soleus atrophy induced by disuse, and Sodium Ferulate and Ligustrazine in high dose showed most efficacy.
2. Hemorheology property and muscle fiber ultrastructure in/out muscle spindle have relationship with physiological property of ground squirrel, this may be one of the reason that anti-muscle atrophy during hibernation.
1.对川芎及川芎中起活血作用的两种主要药效成分(阿魏酸钠和川芎嗪)对后肢去负荷大鼠比目鱼肌萎缩的影响与作用机制进行初步研究。
2.对达乌尔黄鼠冬眠期抗废用性肌萎缩的可能机制进行初步探讨。
研究方法:
1.尾部悬吊法建立大鼠废用性肌萎缩模型,利用免疫组织化学、血液流变学和梭内外肌纤维超微结构三个方面的研究方法观察药物对比目鱼肌的影响。
2.利用血液粘度计和电子显微镜观察并研究达乌尔黄鼠血流变特性及冬眠对梭内外肌纤维超微结构的影响。
研究结果:
1.与后肢去负荷大鼠相比1)高剂量的阿魏酸钠和川芎嗪使比目鱼肌Ⅰ型肌纤维横截面积分别增加了37.3%和39.4%(P＜0.05);2)三种药物均能明显抑制梭外肌纤维MHCⅡ表达水平的升高(P＜0.01);3)使肌梭内核袋2纤维MHC的表达由阳性转变为阴性;4)能明显降低低切变率下的全血粘度;5)对梭内外肌纤维超微结构的改变有明显的抑制作用。
2.1)冬眠中黄鼠全血粘度较活跃期升高,出眠时下降;2)尾部悬吊对黄鼠的血流变特性无明显改变:3)梭内外肌纤维超微结构在冬眠中无较大改变,出眠时较冬眠中加重。
研究结论:
1.川芎及其两种主要药效成分阿魏酸钠与川芎嗪均能不同程度地对抗废用性肌萎缩的发生,以高剂量的阿魏酸钠和川芎嗪药效最为明显。
2.黄鼠血流变特性和梭内外肌纤维超微结构的改变与其生理特性相关,这可能也是其冬眠过程中抗肌萎缩的原因之一。
Objective:
1. To study the effects and their mechanism of Ligusticum chuanxiong and its two components (Sodium Ferulate and Ligustrazine as main efficacy components in Ligusticum chuanxiong for invigorating blood circulation) on muscle atrophy in a hind limb unloaded rat model.
2. To study the ground squirrel's possible mechanisms of anti-muscle atrophy during hibernation.
Methods:
1. The tail-suspended rats were subjected to a 14-days disuse, immunohistochemic, hemorheology property and muscle fiber ultrastructure in/out muscle spindle were used to study the effects of medicines on soleus muscle.
2. Blood viscometer and Electron microscope were used to investigate the ground squirrel's hemorheology property and muscle fiber ultrastructure in/out muscle spindle during hibernation.
Results:
1. Compared with HLU+W, 1) the CSA of soleus type I fibers in HLU+SfH and HLU+TmpH increased by 37.3% and 39.4% respectively (P<0.05); 2) expression level of MHCII were inhibited in all treatment groups (P<0.01); 3) expression of MHC in nuclear bag 2 fiber were altered from positive to negative; 4) the blood viscosity in low shear rates decreased obviously (P<0.001), even near to CON's; 5) and show the inhibited effect on change of muscle fiber ultrastructure in/out muscle spindle.
2. 1) Compare with Aut-A, whole blood viscosity increase during hibernation and decrease after hibernation. 2) Hemorheology property have no significant change after hindlimb-unloading. 3) Muscle fiber ultrastructure in/out muscle spindle have no significant change during hibernation, and after hibernation it worse than H-m.
Conclusion:
1. Ligusticum chuanxiong and its two main efficacy components (Sodium Ferulate and Ligustrazine) can prevent soleus atrophy induced by disuse, and Sodium Ferulate and Ligustrazine in high dose showed most efficacy.
2. Hemorheology property and muscle fiber ultrastructure in/out muscle spindle have relationship with physiological property of ground squirrel, this may be one of the reason that anti-muscle atrophy during hibernation.
引文
党凯.冬眠黄鼠比目鱼肌肌纤维类型与收缩功能的影响[D].西安:西北大学硕士论文.2007
刁颖,祁冲,袁丽丽.哺乳动物的冬眠及其影响因素[J].生物学通报,2006,41(8):14-16
董颀,沈羡云,孟京瑞等.四组复方中药对模拟失重大鼠血液流变性的影响[J].微循环学杂志,2005,15(3):41-43
冯班.废用对达乌尔黄鼠趾长伸肌形态结构和功能影响的研究[D].西安:西北大学硕士论文.2008
胡清照,高云芳,曹晋.不同浓度四君子汤对尾部悬吊大鼠比目鱼肌肌萎缩的影响[J].航天医学与医学工程,2009,22(1):9-12
高云芳.几种活血类与拟胆碱类药物对废用性肌萎缩的防护作用及其机制的研究[D].西安:西安交通大学博士论文.2005
高云芳,樊小力,何志仙等.川芎嗪和黄芪对尾部悬吊大鼠比目鱼肌肌球蛋白ATP酶活性及肌萎缩的影响[J].航天医学与医学工程,2005a,18(4):262-266
郭绮云,葛兆希.运动疗法加综合治疗周围性面神经麻痹的疗效观察[J].中国临床康复,2002,6(5):686-687
黄晨西,林琳,李庆芬.达乌尔黄鼠冬眠期与非冬眠期的产热活性及激素调节[J].北京师范大学学报,2003,39(5):463-687
李梦华,曾缙祥,李端仁.冬眠黄鼠几项血液生理指标的测定和分析[J].天津师大学报,1990,15:60-63
刘光远,沈羡云,陈涤明.重力生理学[M].北京:国防工业出版社1993,154-158,214-217
刘小团,李庆芬,黄晨西等.达乌尔黄鼠冷暴露、冬眠及激醒时的外周甲状腺激素水平变化[J].动物学报,2001,47(5):502-507
刘云等.中华鳖冬眠期与活动期部分生理生化性质的比较研究[J].四川大学学报,2002,4:93-95
马孝武.去负荷比目鱼肌肌纤维萎缩与转型对收缩功能的影响[D].西安:第四军医大学硕士论文.2006
马永烈,孙亚志.失重/模拟失重下肌肉萎缩的研究进展[J].航天医学与医学工程,1997,10(2):149-152
山崎俊明.被动牵张刺激对对大鼠比目鱼肌废用性萎缩的作用[J].理学疗法学,1993,20(2):87-92
沈羡云.航天重力生理学与医学[M].第1版.北京:国防工业出版社,2001:344
沈羡云.航天血瘀症及其防护措施[J].中国空间科学技术,1991,11(6):57-61
沈羡云,陈建和,孟京瑞等.模拟失重对兔血液系统影响的研究[J].航天医学与医学工程,1996,9(3):198-203
沈羡云,董颀,马永烈等.两种中药合剂对悬吊大鼠生理防护效应的初步观察[J].航天医学与医学工程,2002,15(4):250-254
沈羡云,崔伟,马永烈等.30d尾吊大鼠血循环、肌肉和骨骼系统的变化[J].航天医学与医学工程,1999,12(4):277-280
石宏志,李勇枝,沈羡云等.中药复方对模拟失重兔血流变特性及循环系统的调节作用[J].航天医学与医学工程,2005,18(4):251-254
石磊,樊小力,吴苏娣.Na_2 ATP对尾部悬吊大鼠比目鱼肌梭内、外肌纤维MHC表达的影响[J].航天医学与医学工程,2006,19(3):228-230
宋锦苹,钟萍,汪涛等.中药合剂对尾吊大鼠细胞免疫功能的防护作用[J].航天医学与医学工程,2001,14(3):215-217
孙标,刘春,余志斌等.间断性人工重力对抗模拟失重大鼠骨骼肌萎缩的效果[J].中华航空航医学杂志,2000,11(4):248
王琦.肌肉废用对达乌尔黄鼠和大鼠比目鱼肌形态结构与肌纤维类型影响的比较研究[D].西安:西北大学硕士论文.2006
吴苏娣,樊小力.肌梭结构与功能的研究进展[J].生理科学进展,2002,33(2):121-125
吴苏娣,樊小力,唐斌等.尾部悬吊对大鼠比目鱼肌mATP酶活性的影响[J].西安医科大学学报,2001,22(4):298-300
曾缙祥,李端仁,林统先等.冬眠黄鼠代谢、循环、呼吸系统的变化[J].生态学报,1987,7(2):95-101
张海祥.三种营养补剂对后肢去负荷大鼠比目鱼肌形态和功能影响的研究[D].西安:西北大学硕士论文.2007
张霞,魏彦明,陈怀涛.大鼠实验性脾虚证动物模型糖原和琥珀酸脱氢酶的组织化学研究[J].畜牧兽医学报,2004,35(2),237-240
周继斌,樊小力,冯鉴强等.尾部悬吊对大鼠比目鱼肌梭内外肌纤维SDH活性的影响[J].中山医科大学学报,2000,21(4S):17-21
周继斌,樊小力,吴苏娣等.模拟失重对大鼠比目鱼肌重和形态结构的影响[J].解剖学研究,1999,21(2):93-95
周继斌,邢东琦,冯鉴强等.模拟失重影响大鼠比目鱼肌梭内外肌纤维肌球蛋白重链的表达[J].中山医科大学学报,2002,(1):34-37,86
Akima H,Kubo K,Imai M,et al.Inactivity and muscle:effect of resistance training during bed rest on muscle size in the lower limb[J].Acta Physiol Stand,2001,172(4):269-278
Akima H,Kuno S,Suzuki Y,et al.Effects of 20 days of bed rest on physiological cross-sectional area of human thigh and leg muscles evaluated by magnetic resonance imaging[J].Gravit Physiol,1997,4(1):S15-21
Alfrey CP, Udden MM, Leach-Huntoon C, et al. Control of red blood cell mass in spaceflight [J]. Appl Physiol, 1996 81(1):98-104
Allebban Z, Gibson LA, Lange RD, et al. Effects of spaceflight on rat erythroid parameters[J]. Appl Physiol, 1996, 81(3): 117-122
Anderson J, Almeida-Silveira MI, Perot C. Reflex and muscular adaptations in rat soleus muscle after hindlimb suspension[J]. Exp Biol, 1999, 202:2701-2707
Andersen NA, Mesch U, Lovell DJ, et al. The effects of sex, season, and hibernation on haematology and blood viscosity of fee-ranging echidnas (Tachyglossus aculeatus) [J]. Canadian Journal of Zoology, 2000,78(2): 174-181
Andrews MT, Squire TL, Bowen CM. Low-temperature carbon utilization is regulated by novel gene activity in the heart of a hibernating mammal[J]. Physiology, 1998, 95:8392-8397
Appell HJ, Duarte AR, Soares JMC. Supplementation of vitamin E may attenuate skeletal muscle immobilization atrophy [J]. Int J Sports Med, 1997,18:157-160
Appell HJ. Morphology of immobilized skeletal muscle and the effects of a pre- and post immobilization remaining program [J]. Int J Sports Med, 1986, 7(1) :6-12
Bamman MM, Clarke MSF, Feeback DL. Impact of resistance exercise during bed rest on skeletal muscle sarcopenia and myosin isoform distribution[J]. Appl Physiol, 1998, 84:157-163
Bleeker MWP, Hopman MTE, Rongen GA, et al. Unilateral lower limb suspension can cause deep venous thrombosis[J]. Am J Physiol Regul Integr Comp Physiol, 2004, 286:R1176-R1177
Bloomfield SA. Changes in musculoskeletal structure and function with prolonged bed rest[J]. Med Sci Sports Exerc, 1997,29(2): 197-206
Booth FW, Lou W, Hamiton MT, et al. Cytochrome cmRNA in skeletal muscles of immobilized limbs [J]. Appl Physiol, 1996, 81(5):1941-1945
Booth FW. Physiologic and biochemical effects of immobilization on muscle[J]. Clin Orthop, 1987, 6(219): 15-20
Caiozzo VJ, Haddad F, Baker MJ, et al. Microgravity-induced transformations of myosin isoforms and contractile properties of skeletal muscle [J]. Appl Physiol, 1996, 81(1):123-132
Carmeli E et al. Effect of growth hormone on gastrochemins muscle of aped rats after imnobilization: biochem is try and morphology [J]. Appl physiology, 1993, 75:1529
Clarke MS, Bamman MM, Feeback DL. Bed rest decreases mechanically induced myofiber wounding and consequent wound-mediated FGF release[J]. Appl Physiol, 1998, 85(2):593-600
Desaphy JF, Pierno S, Liantonio A, et al. Recovery of the soleus muscle after short- and long-term disuse induced by hindlimb unloading: effects on electrical properties and myosin heavy chain profile [J]. Neurobiology of Disease,2005, (18):356-365
Desplanches D, et al. Structural and metabolic properties of rat muscle exposed to weightlessness aboard Cosmos 1887[J]. Eur J Appl Physiol Occup Physiol, 1991, 63(3-4):288-292
Deveci D, Egginton S. Differing mechanisms of cold-induced changes in capillary supply in M. tibialis anterior of rats and hamsters[J]. Journal of Experimental Biology, 2002, 205(6):829-840
Edgerton, V. R, R. R. Roy. Neuromuscular adaptations to actual and simulated space flight. In: Handbook of Physiology.Environmental Physiology[J]. Bethesda, MD: Am. Physiol. Soc, 1996,4(32):721-764
Edgerton VR, Zhou M-Y, Ohira Y, et al. Human fiber size and enzymatic properties after 5 and 11 days of spaceflight [J]. Appl Physiol, 1995, 78:1733-1739
Egginton S, Fairney J., Bratcher J. Differential effects of cold exposure on muscle fiber composition and capillary supply in hibernator and non-hibernator rodents[J]. Experimental Physiology, 2001, 86(5):629-639
Endo I, Inoue D. Effect of calcium and vitamin D on skeletal muscle [J]. Clin Calcium, 2003, 13 (7):905-7 Japanese
Falempin M, In-Albon SF. Influence of brief daily tendon vibration on rat soleus muscle in non-weight-bearing situation [J]. Appl Physiol, 1999, 87(1):3-9
Fitts RH, Riley DR, Widrick JJ. Physiology of a microgravity environment invited review: microgravity and skeletal muscle [J]. Appl Physiol, 2000, 89(2):823-839
Fitts RH, Metzger JM, Riley DA, et al. Models of disuse: a comparison of hindlimb suspension and immobilization[J]. Appl Physiol, 1986,60(6):1946-1953
GAO Yunfang, FAN Xiao li. Effects of angelica sinensis on afferent discharge of single muscle spindle in toads [J]. Journal of Xi'an Jiao tong University, 2004,16(2): 178-180
Gao YF, Fan XL. Effects of three Chinese medicines on afferent discharge from Single muscle spindle in toads [J]. Space Med Med Eng, 2005c, 18(1):16-18
Grichko VP, Heywood-Cooksey A, Kidd KR, et al. Substrate profile in rat soleus muscle fibers after hindlimb unloading and fatigue [J]. Appl. Physiol, 2000, 88:473-478
Grigoriev AI, Maksimov GV, Morukov BV, et al. Investigation of erythrocyte shape, plasma membrane fluidity and conformation of haemoglobin haemoporphyrin under the influence of long-term space flight[J]. Gravit Physiol, 2004, Jul;11(2):P79-80
Guezennec CY, Gilson E, Serrurier B. Comparative effects of hindlimb suspension and exercise on skeletal muscle myosin isozymes in rats [J]. Eur J Appl Physiol Occup Physiol, 1990, 60(6):430-435
Haddad F, Herrick RE, Adams GR, et al. Myosin heavy chain expression in rodent skeletal muscle: effects of exposure to zero gravity [J]. Appl Physiol, 1993, 75:2471-2477
Hagbarth KE, Macefield VG. The fusimotor system its role in fatigue [J]. Adv Exp Med Biol, 1995, 384:259-270
Hansen G, et al. Effects of spaceflight on myosin heavy-chain content, fibre morphology and succinate dehydrogenase activity in rat diaphragm [J]. Pflugers Arch, 2004, 448(2):239-247
Harutoshi S, Yoshihiro Y, Kiyohiro S, et al. Different frequency treadmill running in immobilization -induced muscle atrophy and ankle joint contracture of rats [J]. Scandinavian Journal of Medicine & Science in Sports, 2004,14(3): 186
Haus JM, Carrithers JA, Carroll CC, et al. Contractile and connective tissue protein content of human skeletal muscle: effects of 35 and 90 days of simulated microgravity and exercise countermeasures[J]. Am J Physiol Regulatory Integrative Comp Physiol, 2007, 293:1722-1727
Herbert ME, Roy RR, Edgerton VR. Influence of one-week hindlimb suspension and intermittent high load exercise on rat muscles [J]. Exp Neurol, 1988,102: 190-198
Hikida RS, Gollnick PD, Dudley GA, et al. Structure and metabolic characteristics of human skeletal muscle following 30 days of simulated microgravity [J]. Aviation, Space, and Environmental Medicine, 1989,60:664-670
Ishihara A, Kawano F, Ishioka N, et al. Effects of running exercise during recovery from hindlimb unloading on soleus muscle fibers and their spinal motoneurons in rats [J]. Neuroscience Research, 2004,48:119-127
Ivanov AP, Goncharov IB, Davydkin AF, et al. Changes in several indicators of blood rheology in experiments simulating weightlessness [J]. Kosm Biol Aviakosm Med, 1983, 17(6):25-30
Joclyn BG, Chou SM. Core myofibers and related alterations induced in rats' soleus by immobilization in shortened position [J]. Neurosci, 1984, 63(1):267-275
Johansen LB, Gharib C, Allevard AM, et al. Haematocrit, plasma volume and noradrenaline in humans during simulated weightlessness for 42 days [J]. Clin Physiol, 1997, 17(2):203-10
Kaperonis AA, Michelsen CB, Askanazi J, et al. Effects of total hip replacement and bed rest on blood rheology and red cell metabolism[J]. Trauma, 1988, Apr;28(4):453-7
Klika E, Zaitsova A. Changes the structural elements of the organs of bats during periods of activity and hibernation [J]. Arkh Anat Gistol Embriol, 1984, 87(9):47-52
Knox M, Fluckey JD, Bennett P, et al. Hindlimb unloading in adult rats using an alternative tail harness design[J]. Aviat Space Environ Med, 2004, 75(8): 692-696
Kozlovskaya IB, Kreidich YuV, Oganov VS, et al. Pathophysiology of motor functions in prolonged space flights [J]. Acta Astronaut, 1981, 8:1059
Kraemer, W. J, S. J. Fleck, W. J. Evans. Strength and power training: physiological mechanisms of adaptation[J]. Exerc.Sport Sci. Rev, 1996,24: 363-398
Ku Z, Thomason DB. Solues muscle nascent polypeptide chain elongation slows protein synthesis rate during non-weight-bearing activity [J]. Am J Physiol Cell Physiol, 1994, 267 :C115-126
Lampe L, Wienhold K, Meyer G, et al. Effects of simulated microgravity (HDT) on blood fluidity[J]. Appl Physiol, 1992, Oct; 73(4): 1366-9
Liang MJ, He LC, Yang GD. Screening, analysis and in vitro vasodilatation of effective components from Ligusticum Chuanxiong [J]. Life Sci.2005 Nov 26,78(2):128-133
Linderman JK, Talmadge RJ, Gosselink KL, et al. Synergistic ablation does not affect atrophy or altered myosin heavy chain expression in the non-weight bearing soleus muscle[J]. Life Sciences, 1996, 59(10):789-795
Linderman JK, Whittall JB, Gosselink KL, et al. Stimulation of myofibrillar protein synthesis in hindlimb suspended rats by resistance exercise and growth hormone[J]. Life Science, 1995, 57(8):755-762
LI Yongzhi, SHI Hongzhi, FAN Quanchun, et al. Effects of qiang gu kang wei congpound on henmorheology in tail-suspended rats [J]. Space Medicine &Medical Engineering, 2002, 15(5):327-330
Lohuis T.D, Harlowa H.J, Beck T.D.I. Hibernating black bears (Ursus americanus) experience skeletal muscle protein balance during winter anorexia [J]. Comparative Biochemistry and Physiology, 2007:1-9
Majhi SN, Nair VR. Effects of microgravity on microcirculation[J]. Microgravity Sci Technol, 1990, 3(2): 117-20
Marco LD, Perris R, Cozzi MR, et al. Blood clotting in space [J]. Biol Regul Homeost Agents, 2004,18: 187-192
Martin DG, Convertino VA, Goldwater D, et al. Plasma viscosity elevations with simulated weightlessness [J]. Aviat Space Environ Med, 1986, 57(5):426-31
Martin TP, Edgerton VR, Grindeland RE. Influence of spaceflight on rat skeletal muscle [J]. Journal of Applied Physiology, 1988, 65(5):2318-2325
Ma YL, Zhu X, Rivera PM, et al. Absence of cellular stress in brain after hypoxia induced by arousal from hibernation in Arctic ground squirrels [J]. Am J Physiol Regul Integr Comp Physiol, 2005,289(5):R 1297-306
McDonald KS, Fitts RH. Effect of hindlimb unloading on rat soleus fiber force, stiffness, and calcium sensitivity [J]. Appl Physiol, 1995, 79(5): 1796-1802
Morey-Holton ER, Globus RK. Hindlimb unloading rodent model: technical aspects[J]. Appl Physiol, 2002,92:1367-1377
Naito H, Powers SK, Demirel HA, et al. Heat stress attenuates skeletal muscle atrophy in hindlimb -unweighted rats [J]. Appl Physiol, 2000, 88(1):359-363
Ni GX, Cheng HP, Su L, et al. Effects of immobilization on morphology of soleus and gastrocnemius muscles [J]. Zhongguo Kangfu Yixui Zazhi(Chin J Rehabil Med), 2000, 15(3):137-139
Ohira Y, Yoshinaga T, Nomura T, et al. Gravitational unloading effects on muscle fiber size, phenotype and myonuclear number[J]. Adv Space Res, 2002, 30(4):777-781
Otis JS, Roy RR, Edgerton VR, et al. Adaptations in metabolic capacity of rat soleus after paralysis[J]. Appl Physiol, 2004, 96:584-596
Pierno, S., Desaphy, J.-F., Liantonio, A, et al. Changes of chloride ion channel conductance of slow-to-fast fibre type transition during unloading induced muscle disuse [J]. Brain, 2002,125:1510-1521
Poliakov W, Ivanova SM, Noskov VB, et al. Hematological investigations in conditions of long-term space flights[J]. Aviakosm Ekolog Med, 1998, 32(2):9-18
Postnikova GB, Tselikova S.V, Kolaeva S.G, et al. Myoglobin content in skeletal muscles of hibernating ground squirrels rises in autumn and winter [J]. Comparative Biochemistry and Physiology Part A Molecular and Integrative Physiology, 1999,124(1):35-37
Qin L, Appell HJ, Chan KM, et al. Electrical stimulation prevents immobilization atrophy in skeletal muscle of rabbits [J]. Arch Phys Med Rehabil, 1997, 78(5):512-517
Riley DA, Bain JLW, Thompson JL, et al. Thin filament diversity and physiological properties of fast and slow fiber types in astronaut leg muscles[J]. Appl Physiol, 2002, 92:817-825
Riley DA, Ellis S, Giometti JF, et al. Muscle sarcomere lesions and thrombosis after spaceflight and suspension unloading [J]. Appl. Physiol, 1992, 73: 33s-43s
Rourke B.C., Yokoyama Y., Milsom W.K, et al. Myosin isoform expression and MAFbx mRNA levels in hibernating golden-mantled ground squirrels(Spermophilus lateralis)[J]. Physiological and Biochemical Zoology, 2004, 77(4):582-593
Roy RR, Baldwin KM, Edgerton. Response of the neuromuscular unit to spaceflight: what has been learned from the rat model [J]. Exerc Sport Sci Rev, 1996,24:399-425
Saunder DK, Patel KH. Comparison of blood viscosity in red-eared sliders (Trachemysscripta) adapted to cold and room temperature[J]. Journal of Experimental Zoology, 1998, 281(3):157-163
Saunders DK, Roberts AC, Aldrich KJ, et al. Hematological and blood viscosity changes in tail-suspended rats [J]. Aviat Space Environ Med, 2002, Jul;73(7):647-53
Saunder DK, Roberts AC, Ultsch GR. Blood viscosity and hematological changes during prolonged submergence in normoxic water of northern and southern musk turtles (Stemotherus odoratus) [J]. Journal of Experimental Zoology, 2000, 287(7):459-466
Schulte LM, Navarro J, Kandarian SC. Regulation of sarcoplasmic reticulum calcium pump gene expression by hind-limb unweighting[J]. Am J Physiol Cell Physiol, 1993, 264:c1308-c1315
Sheu JR, Hsiao G, Lee YM, et al. Antithrombotic effects of tetramethylpyrazine in vivo experiments[J]. Int J Hematol, 2001,73:393-8
Staron RS, Kraemer WJ, Hikida RS, et al. Comparison of soleus muscles from rats exposed to microgravity for 10 versus 14 days[J]. Histochem Cell Biol, 1998,110:73-80
Steffen JM, Musacchia XJ. Spaceflight effects on adult rat muscle protein, nucleic acid,and amino acid [J]. Am J Physiol, 1986,251:1059
Steffen JM, Koebel DA.Musacchia XJ, et al. Morphometric and metabolic indices of disuse in muscles of hibernating ground squirrels[J]. Comp Biochem Physiol, 1991,99(4):815-819
Stein TP, Schluter MD, Galante AT, et al. Energy metabolism pathways in rat muscle under conditions of simulated microgravity[J]. Nutri Bioch, 2002, (13):471-478
St-Pierre J, Boutilier RG. Aerobic capacity of frog skeletal muscle during hibernation[J]. Physiol Biochem Zool, 2001,74(3):390-7
Tahara T. Effect of retroauricular galvanic stimulation on muscle atrophy in the unweighted rat hindlimb[J]. Nippon Jibiinkoka Gakkai Kaiho, 1994,97(2):268-273
Taillandier D, Aurousseau E, Dominique MD, et al. Coordinate activation of lysosomal, Ca~(2+)-activated and ATP-ubiquitin-dependent proteinases in the unweighted rat soleus muscle [J]. Biochem, 1996,316:65-72
Takanashi G. Effect of endurance training on the compositions of fiber types and myosin light chains in muscles under disuse atrophy [J]. Nippon Seikeigeka Gakkai Zasshi, 1992, 66(1):92-101
Talmadge RJ, Roy RR, Edgerton VR. Distribution of myosin heavy chain isoforms in non-weight-bearing rat soleus muscle fibers[J]. Appl Physiol, 1996, 81:2540-2546
Talmadge RJ, Roy RR, Edgerton VR. Persistence of Hybrid Fibers in RatSoleus After Spinal Cord Transection[J]. The Anatomical Record, 1999,255:188-201
Talmadge RJ, Roy RR. Electrophoretic separation of rat skeletal muscle myosin heavy chain isoforms [J]. Appl Physiol, 1993,75(5):2337-2340
Tang B, Fan XL, Wu SD. Effects of simulated weightlessness on myosin heavy chains expression of soleus muscle spindle in rats [J] Space Medicine & Medical Engineering, 2003,16(4): 235-238
Tang B, Fan XL, Wu SD. Effects of tail suspension on electrophysiological characteristics of muscle spindles in isolated rat soleus muscle[J]. Space Medicine & Medical Engineering, 2004, 17(1): 1-6
Tavassoli M. Anemia of spaceflight [J]. Blood. 1982,60 (5):1059-1067
Thomason DB, Booth FW. Atrophy of the soleus muscle by hindlimb unweighting[J]. Appl Physiol, 1990,68:1-12
Tinker D.B., Harlow H.J, Beck T.D.I. Pritein use and muscle-fiber changes in free-ranging, hibernating black bears[J]. Physiological Zoology, 1998, 71(4):414-424
Vandenburgh H, Del TM, Shansky J, et al. Attenuation of skeletal muscle wasting with recombinant human growth hormone secreted from a tissue-engineered bioartificial muscle [J]. Hum Gene Ther, 1998, 9 (17):2555-2564
Vikhlyantsev I.M., Malyshev S.L., Shenkman B.S., et al. The behavior of titin and the proteins of its family from skeletal muscles of ground squirrel (Citellus undulatus) during hibernation and rats under conditions of simulated microgravity [J]. Biofizika, 2004,49(6):995-1002
Volodina AV, Pozdnyakov OM. Structural and functional rearrangement of muscle spindles in rats under conditions or zero gravity [J].Bull Exp Biol Med, 2004,137(1):92-97
Wang BH, Ou-Yang JP. Pharmacological actions of sodium ferulate in cardiovascular system [J]. Cardiovasc Drug Rev, 2005,23:161-72
Wickler S.J, Hoyt D.F, Breukelen F.V. Disuse atrophy in the hibernating golden-mantled ground squirrel, Spermophilus lateralis[J]. Am J Physiol Regul Integr Comp Physiol, 1991,261:R1214-R1217
Widrick JJ, Knuth ST, Norenberg KM, et al. Effect of a 17 day spaceflight on contractile properties of human soleus muscle fibres[J]. Physiol (Lond), 1999, 516:915-930
Wiekler S.J, Hoyt D.F, Breukelen F.V. Disuse atrophy in the hibernating golden-mantled ground squirrel, Spermophilus lateralis [J]. Am J Physiol Regul Integr Comp Physiol, 1991,261 :R1214-R1217
Williams PE. Use of intermittent stretch in the prevention of serial sarcomere loss in immoblised muscle [J]. Ann rheum Dis, 1990,49(5):316-317
Witzmann FA. Soleus muscle atrophy in rats in induced by cast immobilization: lack of effect by anabolic steroids[J]. Arch Phys Med Rehabil, 1988, 69(2):81-85
Yoshida N, Saiyo K, Sasa T, et al. Electrical stimulation prevents deterioration of the oxidative capacity of disuse atrophied muscles in rats [J]. Aviat Space Environ Med, 2003,74(3):207-211
Zhang HX, He ZX, Gao YF, et al. Muscle composition after 14-day hind limb unloading in rats: Effects of two herbal compounds [J]. Aviation, Space, and Environmental Medicine, 2007, 10:1-6
刁颖,祁冲,袁丽丽.哺乳动物的冬眠及其影响因素[J].生物学通报,2006,41(8):14-16
董颀,沈羡云,孟京瑞等.四组复方中药对模拟失重大鼠血液流变性的影响[J].微循环学杂志,2005,15(3):41-43
冯班.废用对达乌尔黄鼠趾长伸肌形态结构和功能影响的研究[D].西安:西北大学硕士论文.2008
胡清照,高云芳,曹晋.不同浓度四君子汤对尾部悬吊大鼠比目鱼肌肌萎缩的影响[J].航天医学与医学工程,2009,22(1):9-12
高云芳.几种活血类与拟胆碱类药物对废用性肌萎缩的防护作用及其机制的研究[D].西安:西安交通大学博士论文.2005
高云芳,樊小力,何志仙等.川芎嗪和黄芪对尾部悬吊大鼠比目鱼肌肌球蛋白ATP酶活性及肌萎缩的影响[J].航天医学与医学工程,2005a,18(4):262-266
郭绮云,葛兆希.运动疗法加综合治疗周围性面神经麻痹的疗效观察[J].中国临床康复,2002,6(5):686-687
黄晨西,林琳,李庆芬.达乌尔黄鼠冬眠期与非冬眠期的产热活性及激素调节[J].北京师范大学学报,2003,39(5):463-687
李梦华,曾缙祥,李端仁.冬眠黄鼠几项血液生理指标的测定和分析[J].天津师大学报,1990,15:60-63
刘光远,沈羡云,陈涤明.重力生理学[M].北京:国防工业出版社1993,154-158,214-217
刘小团,李庆芬,黄晨西等.达乌尔黄鼠冷暴露、冬眠及激醒时的外周甲状腺激素水平变化[J].动物学报,2001,47(5):502-507
刘云等.中华鳖冬眠期与活动期部分生理生化性质的比较研究[J].四川大学学报,2002,4:93-95
马孝武.去负荷比目鱼肌肌纤维萎缩与转型对收缩功能的影响[D].西安:第四军医大学硕士论文.2006
马永烈,孙亚志.失重/模拟失重下肌肉萎缩的研究进展[J].航天医学与医学工程,1997,10(2):149-152
山崎俊明.被动牵张刺激对对大鼠比目鱼肌废用性萎缩的作用[J].理学疗法学,1993,20(2):87-92
沈羡云.航天重力生理学与医学[M].第1版.北京:国防工业出版社,2001:344
沈羡云.航天血瘀症及其防护措施[J].中国空间科学技术,1991,11(6):57-61
沈羡云,陈建和,孟京瑞等.模拟失重对兔血液系统影响的研究[J].航天医学与医学工程,1996,9(3):198-203
沈羡云,董颀,马永烈等.两种中药合剂对悬吊大鼠生理防护效应的初步观察[J].航天医学与医学工程,2002,15(4):250-254
沈羡云,崔伟,马永烈等.30d尾吊大鼠血循环、肌肉和骨骼系统的变化[J].航天医学与医学工程,1999,12(4):277-280
石宏志,李勇枝,沈羡云等.中药复方对模拟失重兔血流变特性及循环系统的调节作用[J].航天医学与医学工程,2005,18(4):251-254
石磊,樊小力,吴苏娣.Na_2 ATP对尾部悬吊大鼠比目鱼肌梭内、外肌纤维MHC表达的影响[J].航天医学与医学工程,2006,19(3):228-230
宋锦苹,钟萍,汪涛等.中药合剂对尾吊大鼠细胞免疫功能的防护作用[J].航天医学与医学工程,2001,14(3):215-217
孙标,刘春,余志斌等.间断性人工重力对抗模拟失重大鼠骨骼肌萎缩的效果[J].中华航空航医学杂志,2000,11(4):248
王琦.肌肉废用对达乌尔黄鼠和大鼠比目鱼肌形态结构与肌纤维类型影响的比较研究[D].西安:西北大学硕士论文.2006
吴苏娣,樊小力.肌梭结构与功能的研究进展[J].生理科学进展,2002,33(2):121-125
吴苏娣,樊小力,唐斌等.尾部悬吊对大鼠比目鱼肌mATP酶活性的影响[J].西安医科大学学报,2001,22(4):298-300
曾缙祥,李端仁,林统先等.冬眠黄鼠代谢、循环、呼吸系统的变化[J].生态学报,1987,7(2):95-101
张海祥.三种营养补剂对后肢去负荷大鼠比目鱼肌形态和功能影响的研究[D].西安:西北大学硕士论文.2007
张霞,魏彦明,陈怀涛.大鼠实验性脾虚证动物模型糖原和琥珀酸脱氢酶的组织化学研究[J].畜牧兽医学报,2004,35(2),237-240
周继斌,樊小力,冯鉴强等.尾部悬吊对大鼠比目鱼肌梭内外肌纤维SDH活性的影响[J].中山医科大学学报,2000,21(4S):17-21
周继斌,樊小力,吴苏娣等.模拟失重对大鼠比目鱼肌重和形态结构的影响[J].解剖学研究,1999,21(2):93-95
周继斌,邢东琦,冯鉴强等.模拟失重影响大鼠比目鱼肌梭内外肌纤维肌球蛋白重链的表达[J].中山医科大学学报,2002,(1):34-37,86
Akima H,Kubo K,Imai M,et al.Inactivity and muscle:effect of resistance training during bed rest on muscle size in the lower limb[J].Acta Physiol Stand,2001,172(4):269-278
Akima H,Kuno S,Suzuki Y,et al.Effects of 20 days of bed rest on physiological cross-sectional area of human thigh and leg muscles evaluated by magnetic resonance imaging[J].Gravit Physiol,1997,4(1):S15-21
Alfrey CP, Udden MM, Leach-Huntoon C, et al. Control of red blood cell mass in spaceflight [J]. Appl Physiol, 1996 81(1):98-104
Allebban Z, Gibson LA, Lange RD, et al. Effects of spaceflight on rat erythroid parameters[J]. Appl Physiol, 1996, 81(3): 117-122
Anderson J, Almeida-Silveira MI, Perot C. Reflex and muscular adaptations in rat soleus muscle after hindlimb suspension[J]. Exp Biol, 1999, 202:2701-2707
Andersen NA, Mesch U, Lovell DJ, et al. The effects of sex, season, and hibernation on haematology and blood viscosity of fee-ranging echidnas (Tachyglossus aculeatus) [J]. Canadian Journal of Zoology, 2000,78(2): 174-181
Andrews MT, Squire TL, Bowen CM. Low-temperature carbon utilization is regulated by novel gene activity in the heart of a hibernating mammal[J]. Physiology, 1998, 95:8392-8397
Appell HJ, Duarte AR, Soares JMC. Supplementation of vitamin E may attenuate skeletal muscle immobilization atrophy [J]. Int J Sports Med, 1997,18:157-160
Appell HJ. Morphology of immobilized skeletal muscle and the effects of a pre- and post immobilization remaining program [J]. Int J Sports Med, 1986, 7(1) :6-12
Bamman MM, Clarke MSF, Feeback DL. Impact of resistance exercise during bed rest on skeletal muscle sarcopenia and myosin isoform distribution[J]. Appl Physiol, 1998, 84:157-163
Bleeker MWP, Hopman MTE, Rongen GA, et al. Unilateral lower limb suspension can cause deep venous thrombosis[J]. Am J Physiol Regul Integr Comp Physiol, 2004, 286:R1176-R1177
Bloomfield SA. Changes in musculoskeletal structure and function with prolonged bed rest[J]. Med Sci Sports Exerc, 1997,29(2): 197-206
Booth FW, Lou W, Hamiton MT, et al. Cytochrome cmRNA in skeletal muscles of immobilized limbs [J]. Appl Physiol, 1996, 81(5):1941-1945
Booth FW. Physiologic and biochemical effects of immobilization on muscle[J]. Clin Orthop, 1987, 6(219): 15-20
Caiozzo VJ, Haddad F, Baker MJ, et al. Microgravity-induced transformations of myosin isoforms and contractile properties of skeletal muscle [J]. Appl Physiol, 1996, 81(1):123-132
Carmeli E et al. Effect of growth hormone on gastrochemins muscle of aped rats after imnobilization: biochem is try and morphology [J]. Appl physiology, 1993, 75:1529
Clarke MS, Bamman MM, Feeback DL. Bed rest decreases mechanically induced myofiber wounding and consequent wound-mediated FGF release[J]. Appl Physiol, 1998, 85(2):593-600
Desaphy JF, Pierno S, Liantonio A, et al. Recovery of the soleus muscle after short- and long-term disuse induced by hindlimb unloading: effects on electrical properties and myosin heavy chain profile [J]. Neurobiology of Disease,2005, (18):356-365
Desplanches D, et al. Structural and metabolic properties of rat muscle exposed to weightlessness aboard Cosmos 1887[J]. Eur J Appl Physiol Occup Physiol, 1991, 63(3-4):288-292
Deveci D, Egginton S. Differing mechanisms of cold-induced changes in capillary supply in M. tibialis anterior of rats and hamsters[J]. Journal of Experimental Biology, 2002, 205(6):829-840
Edgerton, V. R, R. R. Roy. Neuromuscular adaptations to actual and simulated space flight. In: Handbook of Physiology.Environmental Physiology[J]. Bethesda, MD: Am. Physiol. Soc, 1996,4(32):721-764
Edgerton VR, Zhou M-Y, Ohira Y, et al. Human fiber size and enzymatic properties after 5 and 11 days of spaceflight [J]. Appl Physiol, 1995, 78:1733-1739
Egginton S, Fairney J., Bratcher J. Differential effects of cold exposure on muscle fiber composition and capillary supply in hibernator and non-hibernator rodents[J]. Experimental Physiology, 2001, 86(5):629-639
Endo I, Inoue D. Effect of calcium and vitamin D on skeletal muscle [J]. Clin Calcium, 2003, 13 (7):905-7 Japanese
Falempin M, In-Albon SF. Influence of brief daily tendon vibration on rat soleus muscle in non-weight-bearing situation [J]. Appl Physiol, 1999, 87(1):3-9
Fitts RH, Riley DR, Widrick JJ. Physiology of a microgravity environment invited review: microgravity and skeletal muscle [J]. Appl Physiol, 2000, 89(2):823-839
Fitts RH, Metzger JM, Riley DA, et al. Models of disuse: a comparison of hindlimb suspension and immobilization[J]. Appl Physiol, 1986,60(6):1946-1953
GAO Yunfang, FAN Xiao li. Effects of angelica sinensis on afferent discharge of single muscle spindle in toads [J]. Journal of Xi'an Jiao tong University, 2004,16(2): 178-180
Gao YF, Fan XL. Effects of three Chinese medicines on afferent discharge from Single muscle spindle in toads [J]. Space Med Med Eng, 2005c, 18(1):16-18
Grichko VP, Heywood-Cooksey A, Kidd KR, et al. Substrate profile in rat soleus muscle fibers after hindlimb unloading and fatigue [J]. Appl. Physiol, 2000, 88:473-478
Grigoriev AI, Maksimov GV, Morukov BV, et al. Investigation of erythrocyte shape, plasma membrane fluidity and conformation of haemoglobin haemoporphyrin under the influence of long-term space flight[J]. Gravit Physiol, 2004, Jul;11(2):P79-80
Guezennec CY, Gilson E, Serrurier B. Comparative effects of hindlimb suspension and exercise on skeletal muscle myosin isozymes in rats [J]. Eur J Appl Physiol Occup Physiol, 1990, 60(6):430-435
Haddad F, Herrick RE, Adams GR, et al. Myosin heavy chain expression in rodent skeletal muscle: effects of exposure to zero gravity [J]. Appl Physiol, 1993, 75:2471-2477
Hagbarth KE, Macefield VG. The fusimotor system its role in fatigue [J]. Adv Exp Med Biol, 1995, 384:259-270
Hansen G, et al. Effects of spaceflight on myosin heavy-chain content, fibre morphology and succinate dehydrogenase activity in rat diaphragm [J]. Pflugers Arch, 2004, 448(2):239-247
Harutoshi S, Yoshihiro Y, Kiyohiro S, et al. Different frequency treadmill running in immobilization -induced muscle atrophy and ankle joint contracture of rats [J]. Scandinavian Journal of Medicine & Science in Sports, 2004,14(3): 186
Haus JM, Carrithers JA, Carroll CC, et al. Contractile and connective tissue protein content of human skeletal muscle: effects of 35 and 90 days of simulated microgravity and exercise countermeasures[J]. Am J Physiol Regulatory Integrative Comp Physiol, 2007, 293:1722-1727
Herbert ME, Roy RR, Edgerton VR. Influence of one-week hindlimb suspension and intermittent high load exercise on rat muscles [J]. Exp Neurol, 1988,102: 190-198
Hikida RS, Gollnick PD, Dudley GA, et al. Structure and metabolic characteristics of human skeletal muscle following 30 days of simulated microgravity [J]. Aviation, Space, and Environmental Medicine, 1989,60:664-670
Ishihara A, Kawano F, Ishioka N, et al. Effects of running exercise during recovery from hindlimb unloading on soleus muscle fibers and their spinal motoneurons in rats [J]. Neuroscience Research, 2004,48:119-127
Ivanov AP, Goncharov IB, Davydkin AF, et al. Changes in several indicators of blood rheology in experiments simulating weightlessness [J]. Kosm Biol Aviakosm Med, 1983, 17(6):25-30
Joclyn BG, Chou SM. Core myofibers and related alterations induced in rats' soleus by immobilization in shortened position [J]. Neurosci, 1984, 63(1):267-275
Johansen LB, Gharib C, Allevard AM, et al. Haematocrit, plasma volume and noradrenaline in humans during simulated weightlessness for 42 days [J]. Clin Physiol, 1997, 17(2):203-10
Kaperonis AA, Michelsen CB, Askanazi J, et al. Effects of total hip replacement and bed rest on blood rheology and red cell metabolism[J]. Trauma, 1988, Apr;28(4):453-7
Klika E, Zaitsova A. Changes the structural elements of the organs of bats during periods of activity and hibernation [J]. Arkh Anat Gistol Embriol, 1984, 87(9):47-52
Knox M, Fluckey JD, Bennett P, et al. Hindlimb unloading in adult rats using an alternative tail harness design[J]. Aviat Space Environ Med, 2004, 75(8): 692-696
Kozlovskaya IB, Kreidich YuV, Oganov VS, et al. Pathophysiology of motor functions in prolonged space flights [J]. Acta Astronaut, 1981, 8:1059
Kraemer, W. J, S. J. Fleck, W. J. Evans. Strength and power training: physiological mechanisms of adaptation[J]. Exerc.Sport Sci. Rev, 1996,24: 363-398
Ku Z, Thomason DB. Solues muscle nascent polypeptide chain elongation slows protein synthesis rate during non-weight-bearing activity [J]. Am J Physiol Cell Physiol, 1994, 267 :C115-126
Lampe L, Wienhold K, Meyer G, et al. Effects of simulated microgravity (HDT) on blood fluidity[J]. Appl Physiol, 1992, Oct; 73(4): 1366-9
Liang MJ, He LC, Yang GD. Screening, analysis and in vitro vasodilatation of effective components from Ligusticum Chuanxiong [J]. Life Sci.2005 Nov 26,78(2):128-133
Linderman JK, Talmadge RJ, Gosselink KL, et al. Synergistic ablation does not affect atrophy or altered myosin heavy chain expression in the non-weight bearing soleus muscle[J]. Life Sciences, 1996, 59(10):789-795
Linderman JK, Whittall JB, Gosselink KL, et al. Stimulation of myofibrillar protein synthesis in hindlimb suspended rats by resistance exercise and growth hormone[J]. Life Science, 1995, 57(8):755-762
LI Yongzhi, SHI Hongzhi, FAN Quanchun, et al. Effects of qiang gu kang wei congpound on henmorheology in tail-suspended rats [J]. Space Medicine &Medical Engineering, 2002, 15(5):327-330
Lohuis T.D, Harlowa H.J, Beck T.D.I. Hibernating black bears (Ursus americanus) experience skeletal muscle protein balance during winter anorexia [J]. Comparative Biochemistry and Physiology, 2007:1-9
Majhi SN, Nair VR. Effects of microgravity on microcirculation[J]. Microgravity Sci Technol, 1990, 3(2): 117-20
Marco LD, Perris R, Cozzi MR, et al. Blood clotting in space [J]. Biol Regul Homeost Agents, 2004,18: 187-192
Martin DG, Convertino VA, Goldwater D, et al. Plasma viscosity elevations with simulated weightlessness [J]. Aviat Space Environ Med, 1986, 57(5):426-31
Martin TP, Edgerton VR, Grindeland RE. Influence of spaceflight on rat skeletal muscle [J]. Journal of Applied Physiology, 1988, 65(5):2318-2325
Ma YL, Zhu X, Rivera PM, et al. Absence of cellular stress in brain after hypoxia induced by arousal from hibernation in Arctic ground squirrels [J]. Am J Physiol Regul Integr Comp Physiol, 2005,289(5):R 1297-306
McDonald KS, Fitts RH. Effect of hindlimb unloading on rat soleus fiber force, stiffness, and calcium sensitivity [J]. Appl Physiol, 1995, 79(5): 1796-1802
Morey-Holton ER, Globus RK. Hindlimb unloading rodent model: technical aspects[J]. Appl Physiol, 2002,92:1367-1377
Naito H, Powers SK, Demirel HA, et al. Heat stress attenuates skeletal muscle atrophy in hindlimb -unweighted rats [J]. Appl Physiol, 2000, 88(1):359-363
Ni GX, Cheng HP, Su L, et al. Effects of immobilization on morphology of soleus and gastrocnemius muscles [J]. Zhongguo Kangfu Yixui Zazhi(Chin J Rehabil Med), 2000, 15(3):137-139
Ohira Y, Yoshinaga T, Nomura T, et al. Gravitational unloading effects on muscle fiber size, phenotype and myonuclear number[J]. Adv Space Res, 2002, 30(4):777-781
Otis JS, Roy RR, Edgerton VR, et al. Adaptations in metabolic capacity of rat soleus after paralysis[J]. Appl Physiol, 2004, 96:584-596
Pierno, S., Desaphy, J.-F., Liantonio, A, et al. Changes of chloride ion channel conductance of slow-to-fast fibre type transition during unloading induced muscle disuse [J]. Brain, 2002,125:1510-1521
Poliakov W, Ivanova SM, Noskov VB, et al. Hematological investigations in conditions of long-term space flights[J]. Aviakosm Ekolog Med, 1998, 32(2):9-18
Postnikova GB, Tselikova S.V, Kolaeva S.G, et al. Myoglobin content in skeletal muscles of hibernating ground squirrels rises in autumn and winter [J]. Comparative Biochemistry and Physiology Part A Molecular and Integrative Physiology, 1999,124(1):35-37
Qin L, Appell HJ, Chan KM, et al. Electrical stimulation prevents immobilization atrophy in skeletal muscle of rabbits [J]. Arch Phys Med Rehabil, 1997, 78(5):512-517
Riley DA, Bain JLW, Thompson JL, et al. Thin filament diversity and physiological properties of fast and slow fiber types in astronaut leg muscles[J]. Appl Physiol, 2002, 92:817-825
Riley DA, Ellis S, Giometti JF, et al. Muscle sarcomere lesions and thrombosis after spaceflight and suspension unloading [J]. Appl. Physiol, 1992, 73: 33s-43s
Rourke B.C., Yokoyama Y., Milsom W.K, et al. Myosin isoform expression and MAFbx mRNA levels in hibernating golden-mantled ground squirrels(Spermophilus lateralis)[J]. Physiological and Biochemical Zoology, 2004, 77(4):582-593
Roy RR, Baldwin KM, Edgerton. Response of the neuromuscular unit to spaceflight: what has been learned from the rat model [J]. Exerc Sport Sci Rev, 1996,24:399-425
Saunder DK, Patel KH. Comparison of blood viscosity in red-eared sliders (Trachemysscripta) adapted to cold and room temperature[J]. Journal of Experimental Zoology, 1998, 281(3):157-163
Saunders DK, Roberts AC, Aldrich KJ, et al. Hematological and blood viscosity changes in tail-suspended rats [J]. Aviat Space Environ Med, 2002, Jul;73(7):647-53
Saunder DK, Roberts AC, Ultsch GR. Blood viscosity and hematological changes during prolonged submergence in normoxic water of northern and southern musk turtles (Stemotherus odoratus) [J]. Journal of Experimental Zoology, 2000, 287(7):459-466
Schulte LM, Navarro J, Kandarian SC. Regulation of sarcoplasmic reticulum calcium pump gene expression by hind-limb unweighting[J]. Am J Physiol Cell Physiol, 1993, 264:c1308-c1315
Sheu JR, Hsiao G, Lee YM, et al. Antithrombotic effects of tetramethylpyrazine in vivo experiments[J]. Int J Hematol, 2001,73:393-8
Staron RS, Kraemer WJ, Hikida RS, et al. Comparison of soleus muscles from rats exposed to microgravity for 10 versus 14 days[J]. Histochem Cell Biol, 1998,110:73-80
Steffen JM, Musacchia XJ. Spaceflight effects on adult rat muscle protein, nucleic acid,and amino acid [J]. Am J Physiol, 1986,251:1059
Steffen JM, Koebel DA.Musacchia XJ, et al. Morphometric and metabolic indices of disuse in muscles of hibernating ground squirrels[J]. Comp Biochem Physiol, 1991,99(4):815-819
Stein TP, Schluter MD, Galante AT, et al. Energy metabolism pathways in rat muscle under conditions of simulated microgravity[J]. Nutri Bioch, 2002, (13):471-478
St-Pierre J, Boutilier RG. Aerobic capacity of frog skeletal muscle during hibernation[J]. Physiol Biochem Zool, 2001,74(3):390-7
Tahara T. Effect of retroauricular galvanic stimulation on muscle atrophy in the unweighted rat hindlimb[J]. Nippon Jibiinkoka Gakkai Kaiho, 1994,97(2):268-273
Taillandier D, Aurousseau E, Dominique MD, et al. Coordinate activation of lysosomal, Ca~(2+)-activated and ATP-ubiquitin-dependent proteinases in the unweighted rat soleus muscle [J]. Biochem, 1996,316:65-72
Takanashi G. Effect of endurance training on the compositions of fiber types and myosin light chains in muscles under disuse atrophy [J]. Nippon Seikeigeka Gakkai Zasshi, 1992, 66(1):92-101
Talmadge RJ, Roy RR, Edgerton VR. Distribution of myosin heavy chain isoforms in non-weight-bearing rat soleus muscle fibers[J]. Appl Physiol, 1996, 81:2540-2546
Talmadge RJ, Roy RR, Edgerton VR. Persistence of Hybrid Fibers in RatSoleus After Spinal Cord Transection[J]. The Anatomical Record, 1999,255:188-201
Talmadge RJ, Roy RR. Electrophoretic separation of rat skeletal muscle myosin heavy chain isoforms [J]. Appl Physiol, 1993,75(5):2337-2340
Tang B, Fan XL, Wu SD. Effects of simulated weightlessness on myosin heavy chains expression of soleus muscle spindle in rats [J] Space Medicine & Medical Engineering, 2003,16(4): 235-238
Tang B, Fan XL, Wu SD. Effects of tail suspension on electrophysiological characteristics of muscle spindles in isolated rat soleus muscle[J]. Space Medicine & Medical Engineering, 2004, 17(1): 1-6
Tavassoli M. Anemia of spaceflight [J]. Blood. 1982,60 (5):1059-1067
Thomason DB, Booth FW. Atrophy of the soleus muscle by hindlimb unweighting[J]. Appl Physiol, 1990,68:1-12
Tinker D.B., Harlow H.J, Beck T.D.I. Pritein use and muscle-fiber changes in free-ranging, hibernating black bears[J]. Physiological Zoology, 1998, 71(4):414-424
Vandenburgh H, Del TM, Shansky J, et al. Attenuation of skeletal muscle wasting with recombinant human growth hormone secreted from a tissue-engineered bioartificial muscle [J]. Hum Gene Ther, 1998, 9 (17):2555-2564
Vikhlyantsev I.M., Malyshev S.L., Shenkman B.S., et al. The behavior of titin and the proteins of its family from skeletal muscles of ground squirrel (Citellus undulatus) during hibernation and rats under conditions of simulated microgravity [J]. Biofizika, 2004,49(6):995-1002
Volodina AV, Pozdnyakov OM. Structural and functional rearrangement of muscle spindles in rats under conditions or zero gravity [J].Bull Exp Biol Med, 2004,137(1):92-97
Wang BH, Ou-Yang JP. Pharmacological actions of sodium ferulate in cardiovascular system [J]. Cardiovasc Drug Rev, 2005,23:161-72
Wickler S.J, Hoyt D.F, Breukelen F.V. Disuse atrophy in the hibernating golden-mantled ground squirrel, Spermophilus lateralis[J]. Am J Physiol Regul Integr Comp Physiol, 1991,261:R1214-R1217
Widrick JJ, Knuth ST, Norenberg KM, et al. Effect of a 17 day spaceflight on contractile properties of human soleus muscle fibres[J]. Physiol (Lond), 1999, 516:915-930
Wiekler S.J, Hoyt D.F, Breukelen F.V. Disuse atrophy in the hibernating golden-mantled ground squirrel, Spermophilus lateralis [J]. Am J Physiol Regul Integr Comp Physiol, 1991,261 :R1214-R1217
Williams PE. Use of intermittent stretch in the prevention of serial sarcomere loss in immoblised muscle [J]. Ann rheum Dis, 1990,49(5):316-317
Witzmann FA. Soleus muscle atrophy in rats in induced by cast immobilization: lack of effect by anabolic steroids[J]. Arch Phys Med Rehabil, 1988, 69(2):81-85
Yoshida N, Saiyo K, Sasa T, et al. Electrical stimulation prevents deterioration of the oxidative capacity of disuse atrophied muscles in rats [J]. Aviat Space Environ Med, 2003,74(3):207-211
Zhang HX, He ZX, Gao YF, et al. Muscle composition after 14-day hind limb unloading in rats: Effects of two herbal compounds [J]. Aviation, Space, and Environmental Medicine, 2007, 10:1-6