CXCR2基因多态性及其与奶牛乳房炎和乳品质的关联研究
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摘要
本文采用单链构象多态性分析法，以中国荷斯坦牛、中国西门塔尔牛和通江黄牛为研究对象，研究了CXCR2基因的多态性及其对牛奶品质性状的遗传效应，结论如下：
在扩增的6个片段中共发现3个SNP多态位点，分别位于CXCR2基因序列的684bp、777bp和861bp处。
SNP+684和SNP+777通过PCR-SSCP产生A、B、C三个等位基因。其中等位基因A为优势基因。基因型命名为AA、AB、AC、BB、BC、CC。两个位点在荷斯坦和西门塔尔两个群体中均显示高度多态(PIC＞0.50)，而在通江黄牛中显示中度多态(0.25＜PIC＜0.50)；SNP+861经PCR-SSCP产生E和F两个等位基因，且等位基因E为优势基因。该位点在所研究的三个群体中均显示中度多态(0.25＜PIC＜0.50)。
SNP+684和SNP+777两个位点不同基因型在品种间的分布达到显著水平(P＜0.05)。通过最小二乘分析和显著性检验发现，乳品质性状中除总蛋白之外，AA型和AB型的乳品质性状极显著(P＜0.01)或显著(P＜0.05)优于其它基因型。
SNP+861位点不同基因型在品种间差异不显著(P＞0.05)。通过最小二乘分析和显著性检验发现，乳品质性状中除总蛋白之外，EE型奶牛的牛奶品质显著(P＜0.05)优于FF型。
GLM分析结果显示：不同品种牛之间SCS差异极显著(P＜0.01)，品种效应主要来自于中国荷斯坦牛；不同基因型对SCS效应达到极显著水平(P＜0.01)，基因型效应主要来自与BC型、CC型和FF型；胎次对SCS的效应差异不显著。
In this research, the polymorphisms of cattle Interleukin-8 receptorβ(IL-8Rβ,orCXCR.2) gene and the relationship between the polymorphisms of cattle CXCR2 gene and cattle mastitis in 160 tattles including Chinese Holstain cattle, Chinese simmental cattle and Tongjiang huangniu were detected by PCR-SSCP test with six different pairs of primers. Based on the detection of polymophisms, the effects of different genotypes on milk qulitaty were analyzed.
There were 3 Single Nucleotide Polymorphism(SNP) loeis, SNP＋684, SNP＋777, SNP＋861. There were three alleles in SNP＋684 and SNP＋777: A, B, and C. The allele A was the dominant allele in different breeds, its frequency was significantly higher than that of B and C. And the different genotype composed by the three alleles were significantly different; The quality of milk of genotype AA and AB were great significantly or significantly better than that of another genotype.
In SNP＋861, there were two alleles: E and F. The allele E was the dominantallele in different breeds, its frequency was significantly higher than that of F, while the different genotype composed by the three alleles have no significantly difference.
The results by GLM (least square means) analysis and significant test showed that the cattle milk traits of AA genotype and AB genotype were significant prior to those of other genotypes(P＜0.05). The eattle milk traits of the BC genotype and CC genotype were significant inferior to those of other genotypes(P＜0.05), the cattle milk traits of EE genotype was significant prior to that of FF genotype(P＜0.05). While the content of milk total proteid had no significant difference between any genotype(P＞0.05).
在扩增的6个片段中共发现3个SNP多态位点，分别位于CXCR2基因序列的684bp、777bp和861bp处。
SNP+684和SNP+777通过PCR-SSCP产生A、B、C三个等位基因。其中等位基因A为优势基因。基因型命名为AA、AB、AC、BB、BC、CC。两个位点在荷斯坦和西门塔尔两个群体中均显示高度多态(PIC＞0.50)，而在通江黄牛中显示中度多态(0.25＜PIC＜0.50)；SNP+861经PCR-SSCP产生E和F两个等位基因，且等位基因E为优势基因。该位点在所研究的三个群体中均显示中度多态(0.25＜PIC＜0.50)。
SNP+684和SNP+777两个位点不同基因型在品种间的分布达到显著水平(P＜0.05)。通过最小二乘分析和显著性检验发现，乳品质性状中除总蛋白之外，AA型和AB型的乳品质性状极显著(P＜0.01)或显著(P＜0.05)优于其它基因型。
SNP+861位点不同基因型在品种间差异不显著(P＞0.05)。通过最小二乘分析和显著性检验发现，乳品质性状中除总蛋白之外，EE型奶牛的牛奶品质显著(P＜0.05)优于FF型。
GLM分析结果显示：不同品种牛之间SCS差异极显著(P＜0.01)，品种效应主要来自于中国荷斯坦牛；不同基因型对SCS效应达到极显著水平(P＜0.01)，基因型效应主要来自与BC型、CC型和FF型；胎次对SCS的效应差异不显著。
In this research, the polymorphisms of cattle Interleukin-8 receptorβ(IL-8Rβ,orCXCR.2) gene and the relationship between the polymorphisms of cattle CXCR2 gene and cattle mastitis in 160 tattles including Chinese Holstain cattle, Chinese simmental cattle and Tongjiang huangniu were detected by PCR-SSCP test with six different pairs of primers. Based on the detection of polymophisms, the effects of different genotypes on milk qulitaty were analyzed.
There were 3 Single Nucleotide Polymorphism(SNP) loeis, SNP＋684, SNP＋777, SNP＋861. There were three alleles in SNP＋684 and SNP＋777: A, B, and C. The allele A was the dominant allele in different breeds, its frequency was significantly higher than that of B and C. And the different genotype composed by the three alleles were significantly different; The quality of milk of genotype AA and AB were great significantly or significantly better than that of another genotype.
In SNP＋861, there were two alleles: E and F. The allele E was the dominantallele in different breeds, its frequency was significantly higher than that of F, while the different genotype composed by the three alleles have no significantly difference.
The results by GLM (least square means) analysis and significant test showed that the cattle milk traits of AA genotype and AB genotype were significant prior to those of other genotypes(P＜0.05). The eattle milk traits of the BC genotype and CC genotype were significant inferior to those of other genotypes(P＜0.05), the cattle milk traits of EE genotype was significant prior to that of FF genotype(P＜0.05). While the content of milk total proteid had no significant difference between any genotype(P＞0.05).
引文
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