维生素C缓解圈养大熊猫热应激机理研究
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摘要
近年来，大熊猫的保护研究工作取得了很大进展，就总体而言，我国大熊猫迁地保护目前主要停留在迁地饲养和人工繁育这一阶段，对于热应激对圈养大熊猫的影响还处于探索阶段。本研究以成都大熊猫繁育研究基地圈养的8只成年大熊猫作为试验对象，就热应激对圈养成年大熊猫生理生化指标的影响，以及日粮添加维生素C对圈养大熊猫热应激的缓解问题进行了为期1年左右的研究。主要对血清中TP、Ca~(2+)、Na~+、Cl~-、血红蛋白、白蛋白／球蛋白、葡萄糖、等血液生化指标、大熊猫粪便中皮质醇含量、睡眠中的呼吸频率以及采食量随全年气温变化的情况，以及日粮添加维生素C对大熊猫的影响进行了研究，为人工圈养大熊猫繁育生存中的环境调控，尤其是夏季热应激提供理论依据。
研究结果表明：
(1)气温的升高，熊猫采食量下降显著，呼吸频率上升显著(P<0．05)，皮质醇含量上升极显著(P<0．01)，血液中Ca~(2+)、P、及HGB血红蛋白含量在下降显著(P<0．05)。TP、K~+、Glu、在下降极显著(P<0．01)。
(2)日粮添加维生素C能有效防止大熊猫中暑现象发生，缓解大熊猫热应激，明显增加热应激大熊猫采食量，日增重，明显降低呼吸频率以及皮质醇含量，明显降低热应激对大熊猫的影响。实验组TP、Ca~(2+)、K~+、HGB、Glu含量与对照组及实验组添加维生素C前相比有趋向适温状态的变化。
Generally speaking, we have achieved a lot in the giant panda breeding, however themain achievement of the ex-situ conservation is still at the stage of ex-situ breedingand artificial breed, but the study of the suitable microclimate of the captive giantpandas is still at the explore phase. The main content was about how the microclimate affects the physiology bio-chemical indexes of captive giant pandas and discuss the role and mechanism of anti-heat stress additive vitamin C. The detail content included three parts. The first was about the blood bio-chemic index of the total protein in the blood serum, Ca~(2+)、Na~+、Cl、hemoglobin albumin-globulin ratio、glucose. The second was about the content of fecal cortisol of the giant pandas. The third was on the respiration frequency when the giant pandas were sleeping.
The conclusions were drawn as follows:
1.With the temperature increase in summer giant panda Ca~(2+)、P、HGB decreased significantly (P<0.05),TP、K~+、Glu decreased significantly difference(P<0.01). respiration frequency and fecal cortisol increase significantly(P<0.05).
2. anti-heat stress additive vitamin C could improve the productive performance of giant panda, comparing to the control group, TP、Ca~(2+)、K~+ increased significantly(P<0.01), Glu increased significantly difference(P<.01), fecal cortisol decreased significantly(P<0.05)
研究结果表明：
(1)气温的升高，熊猫采食量下降显著，呼吸频率上升显著(P<0．05)，皮质醇含量上升极显著(P<0．01)，血液中Ca~(2+)、P、及HGB血红蛋白含量在下降显著(P<0．05)。TP、K~+、Glu、在下降极显著(P<0．01)。
(2)日粮添加维生素C能有效防止大熊猫中暑现象发生，缓解大熊猫热应激，明显增加热应激大熊猫采食量，日增重，明显降低呼吸频率以及皮质醇含量，明显降低热应激对大熊猫的影响。实验组TP、Ca~(2+)、K~+、HGB、Glu含量与对照组及实验组添加维生素C前相比有趋向适温状态的变化。
Generally speaking, we have achieved a lot in the giant panda breeding, however themain achievement of the ex-situ conservation is still at the stage of ex-situ breedingand artificial breed, but the study of the suitable microclimate of the captive giantpandas is still at the explore phase. The main content was about how the microclimate affects the physiology bio-chemical indexes of captive giant pandas and discuss the role and mechanism of anti-heat stress additive vitamin C. The detail content included three parts. The first was about the blood bio-chemic index of the total protein in the blood serum, Ca~(2+)、Na~+、Cl、hemoglobin albumin-globulin ratio、glucose. The second was about the content of fecal cortisol of the giant pandas. The third was on the respiration frequency when the giant pandas were sleeping.
The conclusions were drawn as follows:
1.With the temperature increase in summer giant panda Ca~(2+)、P、HGB decreased significantly (P<0.05),TP、K~+、Glu decreased significantly difference(P<0.01). respiration frequency and fecal cortisol increase significantly(P<0.05).
2. anti-heat stress additive vitamin C could improve the productive performance of giant panda, comparing to the control group, TP、Ca~(2+)、K~+ increased significantly(P<0.01), Glu increased significantly difference(P<.01), fecal cortisol decreased significantly(P<0.05)
引文
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