中药+贴敷治疗对兔腰椎间盘IL-1α、IL-1β活性影响的实验研究
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摘要
目的：观察活血祛痛汤及伤科黑药膏贴敷治疗对突出椎间盘组织中IL-1α、IL-1β含量的影响,探寻中医药抗炎止痛机理。方法：将90只新西兰大白兔随机分为5组,每组18只；A组：正常对照组,B组：模型对照组,C组：活血祛痛汤组,D组：伤科黑药膏贴敷组,E组：活血祛痛汤+伤科黑药膏贴敷组；除A组外,B、C、D、E组采用侧方腹膜外入路纤维环切开法手术造模,术后一周开始治疗,分别于治疗满2、4、6周后每组各处死6只,用酶联免疫吸附测定法(ELISA法)检测各组动物椎间盘组织中IL-1α、IL-1β的含量,并进行各组间比较。结果：与A组相比,B、C、D、E组椎间盘组织中IL-1 a、IL-1β含量在2、4、6周升高,有统计学差异(P<0.01),提示造模成功。与B组相比,C、D、E三组椎间盘组织中炎性因子含量在2、4、6周均有不同程度降低,尤其在4、6周降低更加明显、有统计学差异(P<0.05),治疗2周开始起效,4、6周效果最明显；不同时间比较,A、B、C、D四组差别均无统计学意义(P>0.05),提示两种治疗方法疗效无差别；E组炎性因子含量下降,且2周与6周比较有统计学差异(P<0.05),说明两种疗法联合治疗效果优于单一疗法。结论：活血祛痛汤及中药贴敷治疗均能降低突出椎间盘组织中IL-1α、IL-1β含量,抑制炎症和免疫反应,治疗4-6周效果最明显,两种治疗方法综合运用效果更佳。
Objective:To observe the influence of blood soup cured pain and traditional Chinese medicine cured application on pain of herniated disc tissue content of interleukin-1αinterleukin-1β,and the mechanism of anti-inflammatory pain medicine. Methods:90 New Zealand rabbits were randomly divided into 5 groups, each 18; By lateral extraperitoneal approach for modeling anular incision surgery, a week after starting treatment, A group:control group, B group:model group, C group:blood soup cured pain group, D group:Chinese patching group, E group:blood soup cured pain+ Chinese patching group, In the treatment of at least 2,4,6 weeks, each group were sacrificed 6,with the ELISA method to detect the content of interleukin-la-. interleukin-1βof animal disc tissue,and the groups were compared. Results:Compared with A, content of interleukin-1α、interleukin-1βof disc tissue in group B significantly increased in 2,4,6 weeks, a significant difference (P<0.01). Compared with B, content of interleukin-1α、interleukin-1βof C,D, E group were decreased within 2,4,6 weeks, especially in 4,6 weeks, were significantly different (P<0.05); Compared with C, D group, the content of interleukin-1α、interleukin-1βof E group decreased, the groups within 4,6 weeks were significantly different (P<0.05). Conclusion:The blood soup and traditional Chinese medicine cured pain sticking therapy could reduce the content of interleukin-1α、interleukin-1βof herniated disc tissue, inhibit inflammation and immune responses, the most obvious effect of the treatment for 4-6 weeks, the integrated use of better effect.
Objective:To observe the influence of blood soup cured pain and traditional Chinese medicine cured application on pain of herniated disc tissue content of interleukin-1αinterleukin-1β,and the mechanism of anti-inflammatory pain medicine. Methods:90 New Zealand rabbits were randomly divided into 5 groups, each 18; By lateral extraperitoneal approach for modeling anular incision surgery, a week after starting treatment, A group:control group, B group:model group, C group:blood soup cured pain group, D group:Chinese patching group, E group:blood soup cured pain+ Chinese patching group, In the treatment of at least 2,4,6 weeks, each group were sacrificed 6,with the ELISA method to detect the content of interleukin-la-. interleukin-1βof animal disc tissue,and the groups were compared. Results:Compared with A, content of interleukin-1α、interleukin-1βof disc tissue in group B significantly increased in 2,4,6 weeks, a significant difference (P<0.01). Compared with B, content of interleukin-1α、interleukin-1βof C,D, E group were decreased within 2,4,6 weeks, especially in 4,6 weeks, were significantly different (P<0.05); Compared with C, D group, the content of interleukin-1α、interleukin-1βof E group decreased, the groups within 4,6 weeks were significantly different (P<0.05). Conclusion:The blood soup and traditional Chinese medicine cured pain sticking therapy could reduce the content of interleukin-1α、interleukin-1βof herniated disc tissue, inhibit inflammation and immune responses, the most obvious effect of the treatment for 4-6 weeks, the integrated use of better effect.
引文
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