武都县吉石坝养鸡场主要病毒性疾病免疫监测
|
摘要
采用血凝抑制试验(haemagglutination inhibition,HI)和琼脂凝胶免疫扩散试验(agar gel immunodiffusion,AGID)对武都县吉石坝适度规模养鸡场鸡群分别进行了鸡新城疫(Newcastle disease,ND)和传染性法氏囊病(infectious bursal disease,IBD)母源抗体水平的测定,结果显示,ND母源抗体的血凝抑制滴度为4.4,IBD抗体阳性率为55%。以此为基础,参照相关的免疫程序,初步制定了该场的免疫计划。利用HI对ND疫苗的免疫效果进行了抗体水平监测和分析。监测鸡群24、39、59、75、119和135日龄的ND血凝抑制抗体滴度分别为5.20、4.80、5.50、6.25、7.35和8.40。利用AGID对IBD、马立克氏病(Marek's disease,MD)和传染性支气管炎(infectious bronchitis,IB)等三种病毒性传染病疫苗的免疫效果进行了抗体水平监测和分析。监测结果表明:监测鸡群14、19、24、29、34、39、44和49日龄IBD抗体阳性率分别是30%、20%、40%、50%、40%、60%、70%和90%:24、29、34、39、44、59和135日龄MD抗体阳性率分别是0%、10%、40%、40%、30%、0%和0%;24、34和59日龄IB抗体阳性率分别是20%、30%、50%。利用AGID测定了禽流感(avian influenza,AI)和鸡传染性喉气管炎(infectious laryngotracheitis,ILT)血清抗体水平。监测结果显示:3月龄和4月龄鸡群AI抗体阳性率分别是10%和20%;24、49和135日龄ILD抗体阳性率分别是0%、0%和0%。依据试验结果,调整并提出了适合农村适度规模养鸡场的免疫程序。
The maternal antibodies of Newcastle disease(ND) and infectious bursal disease(IBD) were detected respectively by means of haemagglutination Inhibition(HI) and agar gel immunodiffusion(AGID) in the moderate-scaled fowl-run in Jishiba, Wudu county. The results showed that the HI titer on maternal antibody of Newcastle disease virus was 4.4 and the positive rate on antibody of infectious bursal disease virus was 55%. Referring to relative immunization schedule, a primary immunization-plan was established. Using HI, The immune effect of Newcastle Disease vaccines was detected and analyzed. The HI titers on antibodies of ND vaccines when the chickens grew up at 34, 39, 59, 75, 119 and 135 day-old were 5.20,4.80, 5.50, 6.25, 7.35 and 8.40 respectively. AGID was applied to detect and analyze the immune effect of IBD vaccine, MD vaccine and IB vaccine. The antibody positive rates of IBD when the chickens grew up at 14, 19, 24, 29, 34, 39, 44and 49 day-old were 30%, 20%, 40%, 50%, 40%, 60%, 70% and 90% respectively. The antibody positive rates of MD vaccine when the chickens grew up at 24, 29, 34, 39, 44, 59 and 135 day-old were 0%, 10%, 40%, 40%, 30%, 0% and 0% respectively. The antibody positive rates of IB vaccine when the chickens grew up at 24, 34 and 59 day-old were 20%、30% and 50% respectively. AGID was applied to detect the serum antibody level of AI and ILT. The results indicated the antibody positive rates of AI when the chickens grew up at 3 and 4 month-old were 10% and 20% respectively. According to the experimental results, an immunization schedule suitable for the moderate-scaled fowl-run was proposed.
The maternal antibodies of Newcastle disease(ND) and infectious bursal disease(IBD) were detected respectively by means of haemagglutination Inhibition(HI) and agar gel immunodiffusion(AGID) in the moderate-scaled fowl-run in Jishiba, Wudu county. The results showed that the HI titer on maternal antibody of Newcastle disease virus was 4.4 and the positive rate on antibody of infectious bursal disease virus was 55%. Referring to relative immunization schedule, a primary immunization-plan was established. Using HI, The immune effect of Newcastle Disease vaccines was detected and analyzed. The HI titers on antibodies of ND vaccines when the chickens grew up at 34, 39, 59, 75, 119 and 135 day-old were 5.20,4.80, 5.50, 6.25, 7.35 and 8.40 respectively. AGID was applied to detect and analyze the immune effect of IBD vaccine, MD vaccine and IB vaccine. The antibody positive rates of IBD when the chickens grew up at 14, 19, 24, 29, 34, 39, 44and 49 day-old were 30%, 20%, 40%, 50%, 40%, 60%, 70% and 90% respectively. The antibody positive rates of MD vaccine when the chickens grew up at 24, 29, 34, 39, 44, 59 and 135 day-old were 0%, 10%, 40%, 40%, 30%, 0% and 0% respectively. The antibody positive rates of IB vaccine when the chickens grew up at 24, 34 and 59 day-old were 20%、30% and 50% respectively. AGID was applied to detect the serum antibody level of AI and ILT. The results indicated the antibody positive rates of AI when the chickens grew up at 3 and 4 month-old were 10% and 20% respectively. According to the experimental results, an immunization schedule suitable for the moderate-scaled fowl-run was proposed.
引文
[1]周蛟.目前影响中国养鸡业的主要疫病及免疫预防[J].中国家禽,2001,23(11):8.
[2]张学东,杨宜林,等.对我国动物传染病研究的思考[J].中国家禽,2001,23(20):5.
[3]张秀美.家禽疫病诊断与防治技术研究新进展[J].山东家禽,2002增刊 13-16.
[4]傅先强.对当前鸡病防治中若干问题的认识和思考[J].中国家禽,2001,23(24).
[5]刘丽艳.鸡多病因呼吸道病的特点及综合防制[J].中国家禽,2002,24(6)
[6]周应志.甘肃省鸡病现状及防制对策(省兽医总站周应志提供).
[7]连灿,高明远,史永年,等.甘肃省动物疫病诊断试验方法汇编[M].甘肃省动物防疫总站汇编2001年12月:3-86.
[8]甘肃省规模化养鸡场主要疫病防制预案(省动物防疫总站提供).
[9]陈峰,吴孔兴,陈瑞爱,等.不同类型ND疫苗免疫对产蛋鸡ND抗体水平及生产性能的影响[J].中国兽医科技,2003,33(3):6567
[10].杨钧伟,潘茹芳.大于鸡场新城疫免疫程序的确定.中国家禽,2001,23(3):25.
[11]叶岐山,崔力兵,叶晴,等.禽病免疫原理与疫苗应用[M].安徽科学技术出版社,1999:61-64.
[12]沈志强,连京华.21世纪中国禽病控制与食品安全策略(上)[J].山东家禽,2002,6:
[13]李连任,王桂起.当前影响我国养鸡业的主要疫病及免疫预防中若干问题的认识和思考[J].山东家禽,2002,12:21.
[14]徐步,当前禽病发生与流行的特点及其防制对策,中国禽业导刊,1999,16(1):13-165.
[15]杜元钊.禽流感的认识及诊断上的误区[J].易邦生物,2001,4:4.
[16]甘孟候.提高我国禽病防治水平之我见[C].中国畜牧兽医学会家禽传染病学分会第五届全国会员代表大会暨第九次学术研讨会论文集,2001年:20.
[17]刘亚刚,刘世贵,刘晓钢,等.动物病毒性传染病的基本特征[J].四川畜牧兽医,2002,7(29):29.
[18]甘孟侯,我国集约化养禽业疾病的发生和防制对策,中国禽业导刊,1999,16(4):4-5
[19]蔡宝祥,我国禽病防制和研究的发展历程,禽业科技,1996,12(5):3-6
[20]甘孟候,家禽细菌性疫病的发生与防治对策,首届海峡两岸禽病防治研讨会论文专辑,1995,6-15
[21]甘孟侯,刘爵,家禽病毒性疫病的发生与防制对策,第二届海峡两岸禽病防治研讨会论文集,1997,8-15
[2]张学东,杨宜林,等.对我国动物传染病研究的思考[J].中国家禽,2001,23(20):5.
[3]张秀美.家禽疫病诊断与防治技术研究新进展[J].山东家禽,2002增刊 13-16.
[4]傅先强.对当前鸡病防治中若干问题的认识和思考[J].中国家禽,2001,23(24).
[5]刘丽艳.鸡多病因呼吸道病的特点及综合防制[J].中国家禽,2002,24(6)
[6]周应志.甘肃省鸡病现状及防制对策(省兽医总站周应志提供).
[7]连灿,高明远,史永年,等.甘肃省动物疫病诊断试验方法汇编[M].甘肃省动物防疫总站汇编2001年12月:3-86.
[8]甘肃省规模化养鸡场主要疫病防制预案(省动物防疫总站提供).
[9]陈峰,吴孔兴,陈瑞爱,等.不同类型ND疫苗免疫对产蛋鸡ND抗体水平及生产性能的影响[J].中国兽医科技,2003,33(3):6567
[10].杨钧伟,潘茹芳.大于鸡场新城疫免疫程序的确定.中国家禽,2001,23(3):25.
[11]叶岐山,崔力兵,叶晴,等.禽病免疫原理与疫苗应用[M].安徽科学技术出版社,1999:61-64.
[12]沈志强,连京华.21世纪中国禽病控制与食品安全策略(上)[J].山东家禽,2002,6:
[13]李连任,王桂起.当前影响我国养鸡业的主要疫病及免疫预防中若干问题的认识和思考[J].山东家禽,2002,12:21.
[14]徐步,当前禽病发生与流行的特点及其防制对策,中国禽业导刊,1999,16(1):13-165.
[15]杜元钊.禽流感的认识及诊断上的误区[J].易邦生物,2001,4:4.
[16]甘孟候.提高我国禽病防治水平之我见[C].中国畜牧兽医学会家禽传染病学分会第五届全国会员代表大会暨第九次学术研讨会论文集,2001年:20.
[17]刘亚刚,刘世贵,刘晓钢,等.动物病毒性传染病的基本特征[J].四川畜牧兽医,2002,7(29):29.
[18]甘孟侯,我国集约化养禽业疾病的发生和防制对策,中国禽业导刊,1999,16(4):4-5
[19]蔡宝祥,我国禽病防制和研究的发展历程,禽业科技,1996,12(5):3-6
[20]甘孟候,家禽细菌性疫病的发生与防治对策,首届海峡两岸禽病防治研讨会论文专辑,1995,6-15
[21]甘孟侯,刘爵,家禽病毒性疫病的发生与防制对策,第二届海峡两岸禽病防治研讨会论文集,1997,8-15