摔跤、柔道运动员减重期身体机能及某些内分泌指标变化的研究
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摘要
观察自由式摔跤运动员(n=16,对照组=8,减体重组=8) 2周摄入低热饮食物训练减体重和优秀男子柔道运动员(n=13,对照组=7)6周的限制饮食减体重时一般机能指标的变化及机体处于能量负平衡状态下运动员食欲素A、瘦素和神经肽Y水平变化以及自由式摔跤运动员2周减体重期肘膝关节快慢速峰力矩及无氧功变化及与血生长抑素之间的相关性。
     1.食物称重法和24小时回顾法以及使用《运动员及大众膳食营养分析与管理系统》进行分析发现,自由式摔跤运动员2周食用低热饮食进行训练后体重、脂肪体重、体重指数下降;优秀男子柔道运动员6周的限制饮食进行训练减体重期间体重、体脂百分率、脂肪体重、瘦体重和体重指数均下降。运动员体重的下降与能量负平衡和训练中能量消耗有关;
     2.自由式摔跤运动员2周减体重实验后血清睾酮水平下降、皮质醇水平升高和睾酮/皮质醇比值下降,血清CK活性升高;优秀男子柔道运动员6周减体重期间一般机能指标未见明显变化。提示有计划的缓慢减体重对身体机能的影响不像快速减体重那样激烈;
     3.自由式摔跤运动员2周减体重实验后食欲素A水平显著升高、瘦素水平明显下降、优秀男子柔道运动员6周的限制饮食进行训练减体重期间上述神经肽变化与此相似,提示食欲素A和瘦素对限制饮食进行训练减体重作用较敏感,说明机体处于能量负平衡时血中这些物质有相应的改变;
     4.自由式摔跤减体重组运动员肘膝关节快慢速屈伸峰力矩实验后比实验前和对照组显著降低;而相对峰力矩有显著升高趋势;自由摔跤运动员实验前后减体重组与照组比较,平均功率、相对平均功率在实验后下降明显,同时实验后减体重组运动员疲劳指数升高与实验前和对照组有明显差异。提示快速减体重在一定程度上影响机体力量输出;
     5. Western blotting显示,自由式摔跤运动员2周减体重实验前后,减体重组和对照组肌肉生长抑素均未见显著差异;摔跤运动员血中肌肉生长抑素与肘关节快慢伸相对峰力矩间有显著相关和有变化趋势。
The purpose of this study was to investigate the changes of physiological determinants such as testosterone, cortisol and neuropeptides related to energy balance in period of 6 week weight loss trials of elite male Judoists (divided into weight loss group (n=7) and control group (n=6)) who gained rights of final competitions for the 10th national games. It was also arranged to assess changes of hormones, strength and power output possibly related to myostatin concentration of elite male free-style wrestlers (divided into weight loss group (n=8) and control group (n=8)) in 2 weeks lasting weight loss trials.
     1.The elite male Judoists’body weight, percent fat, fat mass, fat free mass and body mass index decreased in 6 week weight loss trials. There were similar reductions of body weight, fat mass and body mass index in elite free-style wrestlers in 2 week weight loss trials. It was concluded that the reduction of body weight related to negative energy balance in weight loss trials of Judoists and results showed that total energy, relative energy consumption significantly decreased. Judoists’relative protein, relative fat, relative carbohydrates, percent energy from protein and fat significantly decreased and percent energy from carbohydrates significantly increased.
     2.There were no alterations in physiological determinants of Judoists in 6 week weight loss trials, but elite free-style wrestlers’testosterone level decreased, cortisol level and creatine kinase activity increased in 2 week weight loss trials. The results suggested that phase of weight loss could have affect the alterations in physiological determinants such as T, C and CK in weight loss trials.
     3.This is the novel study that we found increased orexin A, decreased leptin and no alteration in neuropeptide Y in 6 week weight loss trials of Judoists, and results showed similar trends in 2 week weight loss trials of wrestlers. It suggested that alterations in orexin A and leptin are more sensitive to dietary restricted training protocols of athletes who need to weight reduction, further indication from this study that neuropeptides have their functional alterations in negative energy balance.
     4.In this study, Biodex system 3 was applied to determine the peak torque and relative peak torque of free-style wrestlers in weight loss trials.There were significant reductions of 7.5% and 3.0% in elbow extension peak torque at 60o/s and 180o/s of weight loss group compared with control group respectively, and significant elevations of 6.5% and 8.9% in elbow relative flexion peak torque at 60o/s and 180o/s of weight loss group compared with control group respectively; After weight loss trials, compared with control group, the knee extension peak torque of weight loss group reduced significantly at 60o/s for 14.5%, flexion peak torque reduced at 180o/s for 20.3%, relative extension peak torque at 180o/s for 3.7% and relative flexion peak torque at 180o/s for 15.3%, respectively. This investigation is novel because it is the first study of weight loss wrestlers to investigate the changes of plasma myostatin, western blotting revealed that after weight loss trials, there were no significant alterations in myostatin concentration, but it elevated 7.12%, and there were significant relations between myostatin concentration and 180o/s,60o/s elbow relative extension peak torques, and has a trend of relations between peak torques.
     5.There was no significant difference of the peak power and relative peak power in both groups after 30s Wingate test of Wrestlers, but after weight loss trials there are significant reductions in average power, relative average power and elevation in fatigue index in weight loss group. The results suggested that 2 week caloric restriction and training affect power output of Wrestlers.
引文
[1] Sansone RA, Sawyer R. Weight loss pressure on a 5 year old wrestler[J]. Br J Sports Med, 2005,39(1):e2.
    [2] Horswill CA, Park SH, Roemmich JN. Changes in the protein nutritional status of adolescent wrestlers[J]. Med Sci Sports Exerc, 1990,22(5):599-604.
    [3] Roemmich JN, Sinning WE. Weight loss and wrestling training: effects on nutrition, growth, maturation, body composition, and strength[J]. J Appl Physiol, 1997,82(6):1751-9.
    [4] Roemmich JN, Sinning WE. Weight loss and wrestling training: effects on growth-related hormones[J]. J Appl Physiol, 1997,82(6):1760-4.
    [5] 曲绵域,高云秋,浦钧宗,陈吉棣,田得祥. 实用运动医学[M]. 北京:北京科学技术出版社 p255-261.
    [6] 冯连世. 运动员减体重研究现状[J]. 体育科学, 2005,25(9): 59-68.
    [7] Ravussin E, Lillioja S, Knowler WC, et al. Reduced rate of energy expenditure as a risk factor for body-weight gain[J]. N Engl J Med, 1988,318(8):467-72.
    [8] Degoutte F, Jouanel P, Begue RJ, et al. Food restriction, performance, biochemical, psychological, and endocrine changes in judo athletes[J]. Int J Sports Med, 2006,27(1):9-18.
    [9] Fogelholm GM, Koskinen R, Laakso J, et al. Gradual and rapid weight loss: effects on nutrition and performance in male athletes[J]. Med Sci Sports Exerc, 1993,25(3):371-7.
    [10] Richard B. K, Anthony L, Jose A, Craig B, Conrad E, Mike G, Thomas I, Douglas S, Susan M. K, Brian L, Lonnie M. L, Ron M, Jeffrey R. S, Darryn S. W, Tim N. Z. ISSN exercise and sport nutrition review: research and recommendations[J]. Sports Nutrition Review Journal, 2004,1(1):1-44.
    [11] 王维群. 运动员体重控制时的营养. 见:王维群 主编 徐梅芳,周永平 副主编. 高等学校教材 营养学. 北京:高等教育出版社,2001. 166-169.
    [12] Keesey RE, Hirvonen MD. Body weight set-points: determination and adjustment[J]. J Nutr, 1997,127(9):1875S-1883S.
    [13] Dirks AJ, Leeuwenburgh C. Caloric restriction in humans: potential pitfalls and health concerns[J]. Mech Ageing Dev, 2006,127(1):1-7.
    [14] Pothos EN, Creese I, Hoebel BG. Restricted eating with weight loss selectively decreases extracellular dopamine in the nucleus accumbens and alters dopamine response to amphetamine, morphine, and food intake[J]. J Neurosci, 1995,15(10):6640-50.
    [15] Kyrkouli SE, Stanley BG, Hutchinson R, et al. Peptide-amine interactions in the hypothalamic paraventricular nucleus: analysis of galanin and neuropeptide Y in relation to feeding[J]. Brain Res, 1990,521(1-2):185-91.
    [16] 杨靖辉,李菊香,唐朝枢. 一种新的神经肽-Orexins[J]. 国外医学-生理、病理科学与临床分册, 2002,22(2): 537-539.
    [17] Erdem SR, Demirel HA, Broxson CS, et al. Effect of exercise on mRNA expression of select adrenal medullary catecholamine biosynthetic enzymes[J]. J Appl Physiol, 2002,93(2):463-8.
    [18] Sakurai T, Amemiya A, Ishii M, et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior[J]. Cell, 1998,92(4):573-85.
    [19]Sakurai T, Moriguchi T, Furuya K, et al. Structure and function of human prepro-orexin gene[J].J Biol Chem, 1999,274(25):17771-6.
    [20] Kirchgessner AL, Liu M. Orexin synthesis and response in the gut[J]. Neuron, 1999,24(4):941-51.
    [21]Matsumura T, Nakayama M, Satoh H, et al. Plasma orexin-A levels and body composition in COPD[J]. Chest, 2003,123(4):1060-5.
    [22] Haynes AC, Jackson B, Overend P, et al. Effects of single and chronic intracerebroventricular administration of the orexins on feeding in the rat[J]. Peptides, 1999,20(9):1099-105.
    [23] Ziolkowska A, Spinazzi R, Albertin G, et al. Orexins stimulate glucocorticoid secretion from cultured rat and human adrenocortical cells, exclusively acting via the OX1 receptor[J]. J Steroid Biochem Mol Biol, 2005,96(5):423-9.
    [24] Karteris E, Machado RJ, Chen J, et al. Food deprivation differentially modulates orexin receptor expression and signaling in rat hypothalamus and adrenal cortex[J]. Am J Physiol Endocrinol Metab, 2005,288(6):E1089-100.
    [25] Sakurai T, Amemiya A, Ishii M, et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior[J]. Cell, 1998,92(5):1 page following 696.
    [26] Wu MF, John J, Maidment N, et al. Hypocretin release in normal and narcoleptic dogs after food and sleep deprivation, eating, and movement[J]. Am J Physiol Regul Integr Comp Physiol, 2002,283(5):R1079-86.
    [27] Martins PJ, D'Almeida V, Pedrazzoli M, et al. Increased hypocretin-1 (orexin-a) levels in cerebrospinal fluid of rats after short-term forced activity[J]. Regul Pept, 2004,117(3):155-8.
    [28] Kastin AJ, Akerstrom V. Orexin A but not orexin B rapidly enters brain from blood by simple diffusion[J]. J Pharmacol Exp Ther, 1999,289(1):219-23.
    [29] Hulver MW, Houmard JA. Plasma leptin and exercise: recent findings[J]. Sports Med, 2003,33(7):473-82.
    [30] Jurimae J, Maestu J, Jurimae T. Leptin as a marker of training stress in highly trained male rowers?[J]. Eur J Appl Physiol, 2003,90(5-6):533-8.
    [31] Leal-Cerro A, Garcia-Luna PP, Astorga R, et al. Serum leptin levels in male marathon athletes before and after the marathon run[J]. J Clin Endocrinol Metab, 1998,83(7):2376-9.
    [32] Kohrt WM, Landt M, Birge SJ Jr. Serum leptin levels are reduced in response to exercise training, but not hormone replacement therapy, in older women[J]. J Clin Endocrinol Metab, 1996,81(11):3980-5.
    [33] Gutin B, Ramsey L, Barbeau P, et al. Plasma leptin concentrations in obese children: changes during 4-mo periods with and without physical training[J]. Am J Clin Nutr, 1999,69(3):388-94.
    [34] Barbeau P, Gutin B, Litaker MS, et al. Influence of physical training on plasma leptin in obese youths[J]. Can J Appl Physiol, 2003,28(3):382-96.
    [35] Minokoshi Y, Kim YB, Peroni OD, et al. Leptin stimulates fatty-acid oxidation by activating AMP-activated protein kinase[J]. Nature, 2002,415(6869):339-43.
    [36] Ruderman NB, Saha AK, Kraegen EW. Minireview: malonyl CoA, AMP-activated protein kinase, and adiposity[J]. Endocrinology, 2003,144(12):5166-71.
    [37] Hilton LK, Loucks AB. Low energy availability, not exercise stress, suppresses the diurnal rhythm of leptin in healthy young women[J]. Am J Physiol Endocrinol Metab, 2000,278(1):E43-9.
    [38] van Aggel-Leijssen DP, van Baak MA, Tenenbaum R, et al. Regulation of average 24h human plasmaleptin level; the influence of exercise and physiological changes in energy balance[J]. Int J Obes Relat Metab Disord, 1999,23(2):151-8.
    [39] Weltman A, Pritzlaff CJ, Wideman L, et al. Intensity of acute exercise does not affect serum leptin concentrations in young men[J]. Med Sci Sports Exerc, 2000,32(9):1556-61.
    [40] Kraemer RR, Johnson LG, Haltom R, et al. Serum leptin concentrations in response to acute exercise in postmenopausal women with and without hormone replacement therapy[J]. Proc Soc Exp Biol Med, 1999,221(3):171-7.
    [41] Fisher JS, Van Pelt RE, Zinder O, et al. Acute exercise effect on postabsorptive serum leptin[J]. J Appl Physiol, 2001,91(2):680-6.
    [42] Perusse L, Collier G, Gagnon J, et al. Acute and chronic effects of exercise on leptin levels in humans[J]. J Appl Physiol, 1997,83(1):5-10.
    [43] Elias AN, Pandian MR, Wang L, et al. Leptin and IGF-I levels in unconditioned male volunteers after short-term exercise[J]. Psychoneuroendocrinology, 2000,25(5):453-61.
    [44] Landt M, Lawson GM, Helgeson JM, et al. Prolonged exercise decreases serum leptin concentrations[J]. Metabolism, 1997,46(10):1109-12.
    [45] Koistinen HA, Tuominen JA, Ebeling P, et al. The effect of exercise on leptin concentration in healthy men and in type 1 diabetic patients[J]. Med Sci Sports Exerc, 1998,30(6):805-10.
    [46] Olive JL, Miller GD. Differential effects of maximal- and moderate-intensity runs on plasma leptin in healthy trained subjects[J]. Nutrition, 2001,17(5):365-9.
    [47] Essig DA, Alderson NL, Ferguson MA, et al. Delayed effects of exercise on the plasma leptin concentration[J]. Metabolism, 2000,49(3):395-9.
    [48] Jurimae J, Jurimae T. Plasma leptin responses to prolonged sculling in female rowers[J]. J Sports Med Phys Fitness, 2004,44(1):104-9.
    [49] Jarek Maestu. The perceived recovery-stress state and selected hormonal markers of training stress[D]. Jurgen Steinaker, Juri Allik (Ph.D); Tartu University; 2004.
    [50] Noland RC, Baker JT, Boudreau SR, et al. Effect of intense training on plasma leptin in male and female swimmers[J]. Med Sci Sports Exerc, 2001,33(2):227-31.
    [51] Campfield LA, Smith FJ, Guisez Y, et al. Recombinant mouse OB protein: evidence for a peripheral signal linking adiposity and central neural networks[J]. Science, 1995,269(5223):546-9.
    [52] Schwartz RS, Brunzell JD. Increase of adipose tissue lipoprotein lipase activity with weight loss[J]. J Clin Invest, 1981,67(5):1425-30.
    [53] Woods SC, Chavez M, Park CR, et al. The evaluation of insulin as a metabolic signal influencing behavior via the brain[J]. Neurosci Biobehav Rev, 1996,20(1):139-44.
    [54] Shisheva A, Shechter Y. A dynamic system for suppression and re-expression of insulin and pervanadate bioresponses in rat adipocytes. Treatment with okadaic acid and staurosporine[J]. Biochem Pharmacol, 1994,47(9):1537-44.
    [55] Brief DJ, Davis JD. Reduction of food intake and body weight by chronic intraventricular insulin infusion[J]. Brain Res Bull, 1984,12(5):571-5.
    [56] Pedersen BK, Febbraio M. Muscle-derived interleukin-6--a possible link between skeletal muscle, adipose tissue, liver, and brain[J]. Brain Behav Immun, 2005,19(5):371-6.
    [57] Blundell JE, Stubbs RJ, Hughes DA, et al. Cross talk between physical activity and appetite control: does physical activity stimulate appetite?[J]. Proc Nutr Soc, 2003,62(3):651-61.
    [58] Blundell JE, King NA. Overconsumption as a cause of weight gain: behavioural-physiologicalinteractions in the control of food intake (appetite)[J]. Ciba Found Symp, 1996,201:138-54; discussion 154-8, 188-93.
    [59] Pi-Sunyer FX. Exercise effects on calorie intake[J]. Ann N Y Acad Sci, 1987,499:94-103.
    [60] Melby CL, Schmidt WD, Corrigan D. Resting metabolic rate in weight-cycling collegiate wrestlers compared with physically active, noncycling control subjects[J]. Am J Clin Nutr, 1990,52(3):409-14.
    [61] Schmidt WD, Corrigan D, Melby CL. Two seasons of weight cycling does not lower resting metabolic rate in college wrestlers[J]. Med Sci Sports Exerc, 1993,25(5):613-9.
    [62] Bryner RW, Ullrich IH, Sauers J, et al. Effects of resistance vs. aerobic training combined with an 800 calorie liquid diet on lean body mass and resting metabolic rate[J]. J Am Coll Nutr, 1999,18(2):115-21.
    [63] Astrup A, Gotzsche PC, van de Werken K, et al. Meta-analysis of resting metabolic rate in formerly obese subjects[J]. Am J Clin Nutr, 1999,69(6):1117-22.
    [64] Gura T. Uncoupling proteins provide new clue to obesity's causes[J]. Science, 1998,280(5368):1369-70.
    [65] Friedl KE, Moore RJ, Hoyt RW, et al. Endocrine markers of semistarvation in healthy lean men in a multistressor environment[J]. J Appl Physiol, 2000,88(5):1820-30.
    [66] Eliakim A, Scheett TP, Newcomb R, et al. Fitness, training, and the growth hormone-->insulin-like growth factor I axis in prepubertal girls[J]. J Clin Endocrinol Metab, 2001,86(6):2797-802.
    [67] Strauss RH, Lanese RR, Malarkey WB. Weight loss in amateur wrestlers and its effect on serum testosterone levels[J]. JAMA, 1985,254(23):3337-8.
    [68] Booth A, Mazur AC, Dabbs JM Jr. Endogenous testosterone and competition: the effect of "fasting"[J]. Steroids, 1993,58(8):348-50.
    [69] Kraemer WJ, Fry AC, Rubin MR, et al. Physiological and performance responses to tournament wrestling[J]. Med Sci Sports Exerc, 2001,33(8):1367-78.
    [70] Zachwieja JJ, Ezell DM, Cline AD, et al. Short-term dietary energy restriction reduces lean body mass but not performance in physically active men and women[J]. Int J Sports Med, 2001,22(4):310-6.
    [71] Horswill CA, Hickner RC, Scott JR, et al. Weight loss, dietary carbohydrate modifications, and high intensity, physical performance[J]. Med Sci Sports Exerc, 1990,22(4):470-6.
    [72] Kraemer WJ, Volek JS, Clark KL, et al. Influence of exercise training on physiological and performance changes with weight loss in men[J]. Med Sci Sports Exerc, 1999,31(9):1320-9.
    [73] McPherron AC, Lawler AM, Lee SJ. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member[J]. Nature, 1997,387(6628):83-90.
    [74] Walker KS, Kambadur R, Sharma M, et al. Resistance training alters plasma myostatin but not IGF-1 in healthy men[J]. Med Sci Sports Exerc, 2004,36(5):787-93.
    [75] McPherron AC, Lee SJ. Double muscling in cattle due to mutations in the myostatin gene[J]. Proc Natl Acad Sci U S A, 1997,94(23):12457-61.
    [76] Grobet L, Martin LJ, Poncelet D, et al. A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle[J]. Nat Genet, 1997,17(1):71-4.
    [77] Bogdanovich S, Krag TO, Barton ER, et al. Functional improvement of dystrophic muscle by myostatin blockade[J]. Nature, 2002,420(6914):418-21.
    [78] Zimmers TA, Davies MV, Koniaris LG, et al. Induction of cachexia in mice by systemicallyadministered myostatin[J]. Science, 2002,296(5572):1486-8.
    [79] Gonzalez-Cadavid NF, Taylor WE, Yarasheski K, et al. Organization of the human myostatin gene and expression in healthy men and HIV-infected men with muscle wasting[J]. Proc Natl Acad Sci U S A, 1998,95(25):14938-43.
    [80] Huygens W, Thomis MA, Peeters MW, et al. Linkage of myostatin pathway genes with knee strength in humans[J]. Physiol Genomics, 2004,17(3):264-70.
    [81] Ramazanov Z, Jimenez del Rio M, Ziegenfuss T. Sulfated polysaccharides of brown seaweed Cystoseira canariensis bind to serum myostatin protein[J]. Acta Physiol Pharmacol Bulg, 2003,27(2-3):101-6.
    [82] 正态性检验和两样本方差齐性检验. 见:孙振球 主编 徐勇勇 副主编. 医学统计学(供研究生用). 北京:人民卫生出版社,2002. 43-47.
    [83] Roemmich JN, Sinning WE. Sport-seasonal changes in body composition, growth, power and strength of adolescent wrestlers[J]. Int J Sports Med, 1996,17(2):92-9.
    [84] Jequier E, Tappy L. Regulation of body weight in humans[J]. Physiol Rev, 1999,79(2):451-80.
    [85] Komaki G, Matsumoto Y, Nishikata H, et al. Orexin-A and leptin change inversely in fasting non-obese subjects[J]. Eur J Endocrinol, 2001,144(6):645-51.
    [86] Adam JA, Menheere PP, van Dielen FM, et al. Decreased plasma orexin-A levels in obese individuals[J]. Int J Obes Relat Metab Disord, 2002,26(2):274-6.
    [87] Lopez M, Seoane L, Garcia MC, et al. Leptin regulation of prepro-orexin and orexin receptor mRNA levels in the hypothalamus[J]. Biochem Biophys Res Commun, 2000,269(1):41-5.
    [88] BROZEK J, GRANDE F, ANDERSON JT, et al. DENSITOMETRIC ANALYSIS OF BODY COMPOSITION: REVISION OF SOME QUANTITATIVE ASSUMPTIONS[J]. Ann N Y Acad Sci, 1963,110:113-40.
    [89] 李菊花. 营养调查及其评价. 见:张爱珍. 医学营养学(第二版). 北京:人民卫生出版社,2004. 143-163.
    [90] McMurray RG, Proctor CR, Wilson WL. Effect of caloric deficit and dietary manipulation on aerobic and anaerobic exercise[J]. Int J Sports Med, 1991,12(2):167-72.
    [91] Hackney AC, Sinning WE, Bruot BC. Reproductive hormonal profiles of endurance-trained and untrained males[J]. Med Sci Sports Exerc, 1988,20(1):60-5.
    [92] 包呼格吉乐图, 刘霞, 曾凡星,等. 大运动量训练时自由式摔跤运动员应激激素变化的探讨[J]. 西安体育学院学报, 2005(01): 64-67.
    [93] 包·呼格吉乐图. 摔跤运动员大运动量训练阶段安静时血清激素水平变化及其相关分析[J]. 内蒙古师大学报(自然科学汉文版), 1999(01): 73-78.
    [94] Wabitsch M, Hauner H, Heinze E, et al. Body fat distribution and steroid hormone concentrations in obese adolescent girls before and after weight reduction[J]. J Clin Endocrinol Metab, 1995,80(12):3469-75.
    [95] 曾凡星. 间接判定运动员滥用人生长激素的指标的可行性研究[M]. 北京:北京体育大学出版社,2001. 72-73.
    [96] 曾凡星. 运动中生长激素和睾酮的研究进展. 见:杨锡让,傅浩坚. 运动生理学进展-质疑与思考. 北京:北京体育大学出版社,1999. 169-187.
    [97] Hakkinen K, Pakarinen A, Alen M, et al. Relationships between training volume, physical performance capacity, and serum hormone concentrations during prolonged training in elite weight lifters[J]. Int J Sports Med, 1987,8 Suppl 1:61-5.
    [98] Lac G, Berthon P. Changes in cortisol and testosterone levels and T/C ratio during an endurance competition and recovery[J]. J Sports Med Phys Fitness, 2000,40(2):139-44.
    [99] Urhausen A, Gabriel H, Kindermann W. Blood hormones as markers of training stress and overtraining[J]. Sports Med, 1995,20(4):251-76.
    [100] Doumas BT, Watson WA, Biggs HG. Albumin standards and the measurement of serum albumin with bromcresol green[J]. Clin Chim Acta, 1971,31(1):87-96.
    [101] Overall JE. How many repeated measurements are useful?[J]. J Clin Psychol, 1996,52(3):243-52.
    [102] Keselman HJ, Keselman JC. The analysis of repeated measures designs in medical research[J]. Stat Med, 1984,3(2):185-95.
    [103] 徐勇勇. 重复测量数据的方差分析. 见:孙振球 主编 徐勇勇 副主编. 医学统计学(供研究生用). 北京:人民卫生出版社,2002. 200-211.
    [104] 杨则宜, 焦颖. 运动员减、控体重的科学方法及减体重后比赛期的特殊营养补充[J]. 浙江体育科学, 2000(05): 35-37.
    [105] 归予恒, 杨则宜, 王爱兰. 优秀轻级别拳击运动员减控体重与营养补充方法探讨[J]. 中国运动医学杂志, 2004(04).
    [106] Roberts SB, Fuss P, Evans WJ, et al. Energy expenditure, aging and body composition[J]. J Nutr, 1993,123(2 Suppl):474-80.
    [107] Forbes GB, Drenick EJ. Loss of body nitrogen on fasting[J]. Am J Clin Nutr, 1979,32(8):1570-4.
    [108] Romijn JA, Coyle EF, Sidossis LS, et al. Substrate metabolism during different exercise intensities in endurance-trained women[J]. J Appl Physiol, 2000,88(5):1707-14.
    [109] Winder WW, Hardie DG. AMP-activated protein kinase, a metabolic master switch: possible roles in type 2 diabetes[J]. Am J Physiol, 1999,277(1 Pt 1):E1-10.
    [110] Seidell JC, Muller DC, Sorkin JD, et al. Fasting respiratory exchange ratio and resting metabolic rate as predictors of weight gain: the Baltimore Longitudinal Study on Aging[J]. Int J Obes Relat Metab Disord, 1992,16(9):667-74.
    [111] Smith NJ. Weight control in the athlete[J]. Clin Sports Med, 1984,3(3):693-704.
    [112] Housh TJ, Johnson GO, Housh DJ, et al. The effects of age and body weight on anthropometric estimations of minimal wrestling weight in high school wrestlers[J]. Res Q Exerc Sport, 1990,61(4):375-82.
    [113] Housh TJ, Johnson GO, Kenney KB, et al. Validity of anthropometric estimations of body composition in high school wrestlers[J]. Res Q Exerc Sport, 1989,60(3):239-45.
    [114] Housh TJ, Johnson GO, Thorland WG, et al. Validity and intertester error of anthropometric estimations of body density[J]. J Sports Med Phys Fitness, 1989,29(2):149-56.
    [115] Kelly JM, Gorney BA, Kalm KK. The effects of a collegiate wrestling season on body composition, cardiovascular fitness and muscular strength and endurance[J]. Med Sci Sports, 1978,10(2):119-24.
    [116] Heinonen MV, Purhonen AK, Miettinen P, et al. Apelin, orexin-A and leptin plasma levels in morbid obesity and effect of gastric banding[J]. Regul Pept, 2005,130(1-2):7-13.
    [117] Tomasik PJ, Spodaryk M, Sztefko K. Plasma concentrations of orexins in children[J]. Ann Nutr Metab, 2004,48(4):215-20.
    [118] Sartorio A, Agosti F, Resnik M, et al. Effects of a 3-week integrated body weight reduction program on leptin levels and body composition in severe obese subjects[J]. J Endocrinol Invest, 2003,26(3):250-6.
    [119] Zabrocka L, Raczynska S, Goyke E, et al. BMI is the main determinant of the circulatingleptin in women after vertical banded gastroplasty[J]. Obes Res, 2004,12(3):505-12.
    [120] Toornvliet AC, Pijl H, Frolich M, et al. Insulin and leptin concentrations in obese humans during long-term weight loss[J]. Neth J Med, 1997,51(3):96-102.
    [121] Smalley KJ, Knerr AN, Kendrick ZV, et al. Reassessment of body mass indices[J]. Am J Clin Nutr, 1990,52(3):405-8.
    [122] Steen SN, Brownell KD. Patterns of weight loss and regain in wrestlers: has the tradition changed?[J]. Med Sci Sports Exerc, 1990,22(6):762-8.
    [123] Ida T, Nakahara K, Murakami T, et al. Possible involvement of orexin in the stress reaction in rats[J]. Biochem Biophys Res Commun, 2000,270(1):318-23.
    [124] Kuru M, Ueta Y, Serino R, et al. Centrally administered orexin/hypocretin activates HPA axis in rats[J]. Neuroreport, 2000,11(9):1977-80.
    [125] Clement K, Vaisse C, Lahlou N, et al. A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction[J]. Nature, 1998,392(6674):398-401.
    [126] Boden G, Chen X, Mozzoli M, et al. Effect of fasting on serum leptin in normal human subjects[J]. J Clin Endocrinol Metab, 1996,81(9):3419-23.
    [127] Leibel RL, Rosenbaum M, Hirsch J. Changes in energy expenditure resulting from altered body weight[J]. N Engl J Med, 1995,332(10):621-8.
    [128] 杨锡让,傅浩坚主编. 运动生理学进展[M]. 北京:北京体育大学出版社,1999. 381-382.
    [129] Schwartz MW, Seeley RJ, Campfield LA, et al. Identification of targets of leptin action in rat hypothalamus[J]. J Clin Invest, 1996,98(5):1101-6.
    [130] Erickson JC, Clegg KE, Palmiter RD. Sensitivity to leptin and susceptibility to seizures of mice lacking neuropeptide Y[J]. Nature, 1996,381(6581):415-21.
    [131] Schwartz MW, Seeley RJ, Woods SC, et al. Leptin increases hypothalamic pro-opiomelanocortin mRNA expression in the rostral arcuate nucleus[J]. Diabetes, 1997,46(12):2119-23.
    [132] Kolaczynski JW, Considine RV, Ohannesian J, et al. Responses of leptin to short-term fasting and refeeding in humans: a link with ketogenesis but not ketones themselves[J]. Diabetes, 1996,45(11):1511-5.
    [133] Ahima RS, Prabakaran D, Mantzoros C, et al. Role of leptin in the neuroendocrine response to fasting[J]. Nature, 1996,382(6588):250-2.
    [134] Heiman ML, Ahima RS, Craft LS, et al. Leptin inhibition of the hypothalamic-pituitary-adrenal axis in response to stress[J]. Endocrinology, 1997,138(9):3859-63.
    [135] Bornstein SR, Uhlmann K, Haidan A, et al. Evidence for a novel peripheral action of leptin as a metabolic signal to the adrenal gland: leptin inhibits cortisol release directly[J]. Diabetes, 1997,46(7):1235-8.
    [136] Jarek Maestu. The perceived recovery-stress state and selected hormonal markers of training stress[J].
    [137] Kaye WH, Berrettini W, Gwirtsman H, et al. Altered cerebrospinal fluid neuropeptide Y and peptide YY immunoreactivity in anorexia and bulimia nervosa[J]. Arch Gen Psychiatry, 1990,47(6):548-56.
    [138] Gendall KA, Kaye WH, Altemus M, et al. Leptin, neuropeptide Y, and peptide YY in long-term recovered eating disorder patients[J]. Biol Psychiatry, 1999,46(2):292-9.
    [139] Broqua P, Wettstein JG, Rocher MN, et al. Behavioral effects of neuropeptide Y receptoragonists in the elevated plus-maze and fear-potentiated startle procedures[J]. Behav Pharmacol, 1995,6(3):215-222.
    [140] Morgan CA 3rd, Wang S, Southwick SM, et al. Plasma neuropeptide-Y concentrations in humans exposed to military survival training[J]. Biol Psychiatry, 2000,47(10):902-9.
    [141] Nam SY, Kratzsch J, Kim KW, et al. Cerebrospinal fluid and plasma concentrations of leptin, NPY, and alpha-MSH in obese women and their relationship to negative energy balance[J]. J Clin Endocrinol Metab, 2001,86(10):4849-53.
    [142] Bi S, Scott KA, Hyun J, et al. Running wheel activity prevents hyperphagia and obesity in Otsuka long-evans Tokushima Fatty rats: role of hypothalamic signaling[J]. Endocrinology, 2005,146(4):1676-85.
    [143] Lundberg JM, Martinsson A, Hemsen A, et al. Co-release of neuropeptide Y and catecholamines during physical exercise in man[J]. Biochem Biophys Res Commun, 1985,133(1):30-6.
    [144] Rasmusson AM, Hauger RL, Morgan CA, et al. Low baseline and yohimbine-stimulated plasma neuropeptide Y (NPY) levels in combat-related PTSD[J]. Biol Psychiatry, 2000,47(6):526-39.
    [145] Hanson ES, Levin N, Dallman MF. Elevated corticosterone is not required for the rapid induction of neuropeptide Y gene expression by an overnight fast[J]. Endocrinology, 1997,138(3):1041-7.
    [146] Guidi L, Tricerri A, Vangeli M, et al. Neuropeptide Y plasma levels and immunological changes during academic stress[J]. Neuropsychobiology, 1999,40(4):188-95.
    [147] Bernet F, Dedieu JF, Laborie C, et al. Circulating neuropeptide Y (NPY) and catecholamines in rat under resting and stress conditions. Arguments for extra-adrenal origin of NPY, adrenal and extra-adrenal sources of catecholamines[J]. Neurosci Lett, 1998,250(1):45-8.
    [148] Gajek J, Zysko D. [The influence of single moderate exercise on the sympathetic nervous system activity in patients with essential hypertension][J]. Pol Arch Med Wewn, 2002,108(6):1145-50.
    [149] Lewandowski J, Pruszczyk P, Elaffi M, et al. Blood pressure, plasma NPY and catecholamines during physical exercise in relation to menstrual cycle, ovariectomy, and estrogen replacement[J]. Regul Pept, 1998,75-76:239-45.
    [150] Levenson CW, Moore JB. Response of rat adrenal neuropeptide Y and tyrosine hydroxylase mRNA to acute stress is enhanced by long-term voluntary exercise[J]. Neurosci Lett, 1998,242(3):177-9.
    [151] Sindelar DK, Ste Marie L, Miura GI, et al. Neuropeptide Y is required for hyperphagic feeding in response to neuroglucopenia[J]. Endocrinology, 2004,145(7):3363-8.
    [152] Jensen EW, Espersen K, Kanstrup IL, et al. Exercise-induced changes in plasma catecholamines and neuropeptide Y: relation to age and sampling times[J]. J Appl Physiol, 1994,76(3):1269-73.
    [153] Song TM, Cipriano N. Effects of seasonal training on physical and physiological function on elite varsity wrestlers[J]. J Sports Med Phys Fitness, 1984,24(2):123-30.
    [154] Song TM. Effects of seasonal training on anthropometry, flexibility, strength, and cardiorespiratory function on junior female track and field athletes[J]. J Sports Med Phys Fitness, 1983,23(2):168-77.
    [155] [美]J.萨姆布鲁克,E.F.弗里奇,T.曼妮阿蒂斯. 分子克隆实验指南(第二版)[M]. 北京:科学出版社,1999. 888-898.
    [156] Hickner RC, Horswill CA, Welker JM, et al. Test development for the study of physical performance in wrestlers following weight loss[J]. Int J Sports Med, 1991,12(6):557-62.
    [157] Filaire E, Maso F, Degoutte F, et al. Food restriction, performance, psychological state and lipid values in judo athletes[J]. Int J Sports Med, 2001,22(6):454-9.
    [158] Maffulli N. Making weight: a case study of two elite wrestlers[J]. Br J Sports Med, 1992,26(2):107-10.
    [159] Fleming J, Sharman MJ, Avery NG, et al. Endurance capacity and high-intensity exercise performance responses to a high fat diet[J]. Int J Sport Nutr Exerc Metab, 2003,13(4):466-78.
    [160] Webster S, Rutt R, Weltman A. Physiological effects of a weight loss regimen practiced by college wrestlers[J]. Med Sci Sports Exerc, 1990,22(2):229-34.
    [161] Liu W, Thomas SG, Asa SL, et al. Myostatin is a skeletal muscle target of growth hormone anabolic action[J]. J Clin Endocrinol Metab, 2003,88(11):5490-6.
    [162] Schulte JN, Yarasheski KE. Effects of resistance training on the rate of muscle protein synthesis in frail elderly people[J]. Int J Sport Nutr Exerc Metab, 2001,11 Suppl:S111-8.
    [163] Zachwieja JJ, Smith SR, Sinha-Hikim I, et al. Plasma myostatin-immunoreactive protein is increased after prolonged bed rest with low-dose T3 administration[J]. J Gravit Physiol, 1999,6(2):11-5.
    [164] Yarasheski KE, Bhasin S, Sinha-Hikim I, et al. Serum myostatin-immunoreactive protein is increased in 60-92 year old women and men with muscle wasting[J]. J Nutr Health Aging, 2002,6(5):343-8.
    [165] Wehling M, Cai B, Tidball JG. Modulation of myostatin expression during modified muscle use[J]. FASEB J, 2000,14(1):103-10.
    [166] Carlson CJ, Booth FW, Gordon SE. Skeletal muscle myostatin mRNA expression is fiber-type specific and increases during hindlimb unloading[J]. Am J Physiol, 1999,277(2 Pt 2):R601-6.
    [1] Sansone RA, Sawyer R. Weight loss pressure on a 5 year old wrestler[J]. Br J Sports Med, 2005,39(1):e2.
    [2] Roemmich JN, Sinning WE. Sport-seasonal changes in body composition, growth, power and strength of adolescent wrestlers[J]. Int J Sports Med, 1996,17(2):92-9.
    [3] Roemmich JN, Sinning WE. Weight loss and wrestling training: effects on growth-related hormones[J]. J Appl Physiol, 1997,82(6):1760-4.
    [4] Roemmich JN, Sinning WE. Weight loss and wrestling training: effects on nutrition, growth, maturation, body composition, and strength[J]. J Appl Physiol, 1997,82(6):1751-9.
    [5] Jequier E, Tappy L. Regulation of body weight in humans[J]. Physiol Rev, 1999,79(2):451-80.
    [6] Strauss RH, Lanese RR, Malarkey WB. Weight loss in amateur wrestlers and its effect on serum testosterone levels[J]. JAMA, 1985,254(23):3337-8.
    [7] Komaki G, Matsumoto Y, Nishikata H, et al. Orexin-A and leptin change inversely in fasting non-obese subjects[J]. Eur J Endocrinol, 2001,144(6):645-51.
    [8] Adam JA, Menheere PP, van Dielen FM, et al. Decreased plasma orexin-A levels in obese individuals[J]. Int J Obes Relat Metab Disord, 2002,26(2):274-6.
    [9] Lopez M, Seoane L, Garcia MC, et al. Leptin regulation of prepro-orexin and orexin receptor mRNA levels in the hypothalamus[J]. Biochem Biophys Res Commun, 2000,269(1):41-5.
    [10] Kraemer WJ, Fry AC, Rubin MR, et al. Physiological and performance responses to tournament wrestling[J]. Med Sci Sports Exerc, 2001,33(8):1367-78.
    [11] Gonzalez-Cadavid NF, Taylor WE, Yarasheski K, et al. Organization of the human myostatin gene and expression in healthy men and HIV-infected men with muscle wasting[J]. Proc Natl Acad Sci U S A, 1998,95(25):14938-43.
    [12] Walker KS, Kambadur R, Sharma M, et al. Resistance training alters plasma myostatin but not IGF-1 in healthy men[J]. Med Sci Sports Exerc, 2004,36(5):787-93.
    [13] Huygens W, Thomis MA, Peeters MW, et al. Linkage of myostatin pathway genes with knee strength in humans[J]. Physiol Genomics, 2004,17(3):264-70.

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