摘要
本文主要研究了豌豆皮水溶性膳食纤维(PSDF)的制备、性质和应用。首先采用蛋白酶和高温淀粉酶去除豌豆皮中附着的蛋白质和淀粉得到豌豆皮不溶性膳食纤维(PIDF)。通过筛选得到豌豆皮蛋白质的最佳水解酶是碱性蛋白酶,再由正交试验分析得到碱性蛋白酶最佳的酶解条件:碱性蛋白酶添加量为600U/g豌豆皮、反应时间100min、料液比为1:15,最终PIDF的残余量为2.20%。PIDF的红外光谱有典型的糖类吸收峰;电镜显示,PIDF呈片状结构。
然后采用纤维素酶、木聚糖酶水解PIDF得到豌豆皮水溶性膳食纤维(PSDF),通过响应面设计实验过程优化水解PIDF工艺,利用Design-Expert软件对数据进行分析得到:纤维素酶的最佳酶解条件:纤维素酶添加量为118U/gPIDF,反应时间为4.98h,pH值为4.59,纤维素酶水解得到的豌豆皮水溶性膳食纤维(PSDF1)的得率为36.28% ;木聚糖酶最佳酶解条件:木聚糖酶添加量为119.5U/gPIDF,反应时间为2.31h,pH值为5.10,木聚糖酶水解得到的豌豆皮膳食纤维(PSDF2)的得率平均为48.57%。对所得到的PSDF1和PSDF2进行进一步研究发现,两者表面都具有多孔结构,但是PSDF2的孔状结构更加致密均匀,通过红外光谱得知两者都具有糖类的特征吸收峰。研究比较了PIDF、PSDF1、PSDF2三种膳食纤维的体外抗氧化能力和吸附能力。在抗氧化能力方面,PSDF的抗氧化能力也明显高于PIDF,而且PSDF2清除DPPH?、?OH自由基的能力高于PSDF1,但是PSDF1的还原能力、螯合铁离子能力高于PSDF2;PSDF对脂肪、胆酸钠、胆固醇的吸附能力明显高于PIDF,而PSDF1对脂肪和胆固醇的吸附能力高于PSDF2的吸附能力。并且通过小鼠实验证实PSDF1和PSDF2具有减缓糖尿病小鼠体重下降的作用,降低糖尿病小鼠的血糖。
最后初步探讨了PSDF的应用,将PSDF1、PSDF2应用在米乳饮料中,试验表明,PSDF1在添加量为6%时感官评价得分最高,PSDF2的添加量为4%时感官评价得分最高,并且在最佳配方的基础上对饮料进行保藏试验,经检验无质量问题。
The method of preparation, the property and the application of PSDF( pea-hulls soluble dietary fiber) were mainly discussed in this thesis, PIDF(pea-hulls insoluble dietary fiber) was obtained with the Protease and Amylase to remove the protein and starch attached in the pea-hulls. Alkaline protease was chosen as the optimum hydrolase of pea-hulls’protein, through the orthogonal experiment, the optimum hydrolysis conditions were determined as: the addition of alkaline protease was 600U/g pea-hulls, reaction time was 100min, solid/solvent was 1:15. And finally the residual protein was 2.20%.A typical carbohydrate absorption was detected in IR(Infrared Radiation)of PIDF, a sheet structure of PIDF was revealed by SEM(scanning electron microscopy).
Then, PSDF was made with xylanase and cellulase, the hydrolysis conditions were optimized with the method of Central composite design, through statistical data analysis of Design-Expert experiment, the optimum hydrolysis conditions for cellulase were: the addition of cellulase was 118U/g PSDF, reaction time was 4.98h, pH value was 4.59, And the average rate of obtained protein hydrolyzed with cellulose (PSDF1)was 36.28%, the optimum hydrolysis conditions for xylanase were: the addition of xylanase was 119.5U/g PSDF, reaction time was 2.31h, pH value was 5.10, And the average rate of obtained protein hydrolyzed with xylanase (PSDF2)was 48.57%. After the further research on PSDF1 and PSDF2, the porous structure were found on both their surface and the pores of PSDF2 were more dense than PSDF1. Then the featured carbohydrate absorptions were obtained in both IR spectrum, and the in-vitro antioxidant capacity and adsorption capacities of three dietary fibers(PIDF、PSDF1、PSDF2) were compared, the results showed: PSDF2 had a higher capacity in DPPH?、?OH radicals’scavenging, but was much inferior in reducing and chelating with iron ion capacities, the adsorption of PSDF to fat , cholate and cholesterol were stronger than PIDF and the adsorption of PSDF1 to fat , cholesterol were stronger than PSDF2. PSDF1 and PSDF2 has been confirmed have the function to low weight loss and reduce blood sugar of diabetes mice through the mice experimental.
Finally, PSDF1 and PSDF2 were applied to rice-milk drink production which was a preliminary exploration in PSDF’s application, the results indicated that it scored highest when the addition of PSDF1, PSDF2 were 6% and 4% respectively, in addition, the drink had passed the storage test and verification on the basis of the optimum formula and tested error free.
引文
1.郑建仙.功能性膳食纤维[M].北京:化工工业出版社,2005,1-4
2.郑建仙,耿立萍,高孔荣.利用车蔗渣制备高活性膳食纤维添加剂的研究[J].食品与发酵工业,2006(3):58-61
3.魏红,徐宏伟,肿红舰,等.膳食纤维的应用及检测方法[J].海峡预防医学,2004,10(2).25-27.
4.BONN. Report of the 26th Session of the CODEX Committee on nutrition and foods for special dietary use.[R/OL]
5.迟玉杰,张瑛卓,闫明武.影响膳食纤维生理功能的内外因素[J].中国乳业工业,2000(2):26-28
6.罗绯.豆皮水溶性膳食纤维提取、生理功能及物化性质的研究[D]:[硕士学位论文]湖南:湖南农业大学,2008:1-2
7.Mark L Dreher.Handbook of Dietary Fiber[M] Evansville, Indiana: Mead Johnson Nutritionals/Bristol-Myers Squibb Company:2001:1-4
8.Hadden Grahan. Extraction of soluble dietary fiber[J]. Agric food chemistry.2006(36):497
9.Marlett J A,McBurney M I,Slavin J L.Position of the American Dietetic Association:Health Implications of Dietary Fiber.Journal of the American Dietetic Association,2002, 102(7):993-1000
10.Galisteo M, Duarteand J, Zarzuelo A. Effects of dietary fibers on disturbances clustered in the metabolic syndrome. Doi:10.1016/j.jnutbio.2007.02.009
11.Kainuma S, Nakamura N,Yamamoto M.Method of preparing water-soluble dietary fiber. US Patent,5622738.1997-04-22
12.Peters U,Sinha R,Chatterjee N,et al.Dietary fiber and colorectal adenoma in a colorectal cancer early detection programme.The Lancet,2003,361(9368):1491~1495
13.Wlliams C L.Dietary fiber in childhood.The Journal of Pediatrics,2006,149(5):S121~S130
14.李慧娜.莲藕渣中膳食纤维的制备及其功能活性研究[D]:[硕士学位论文]湖北:华中农业大学,2009:6-7
15.Galisteo M, Duarteand J, Zarzuelo A. Effects of dietary fibers on disturbances clustered in the metabolic syndrome. Doi:10.1016/j.jnutbio.2007.02.009
16.林德荣.可溶性膳食纤维提取、理化性质及其生理功能的研究[D] :[硕士学位论文]江西:南昌大学,2008:8-10
17.Cum mings J H,Edmond L M,Magee E A.Dietary carbohydrates and health:Do we still need the fiber concept?Clinical Nutrition Supplements,2004,1(2):5-17
18.Giacco R,Clemente G,Riccardi,G.Dietary fiber in treatment of diabetes: myth or reality .Digestive and Liver Disease,2002,34(2):S140-S144
19.Kalkwarf H, Bell R C,Khoury J C,et al.Dietary Fiber Intakes and Insulin Requirements Pregnant Women with Type 1 Diabetes.Journal of the American Dietetic Association,2001, 101(3):305-310
20.Liu S,Buring J E,Sesso H D,et al.A prospective study of dietary fiber intake and risk of cardiovasular disease among women.Journal of the American College of Cardiology,2002, 39(1):49-56
21.Li J,Takashi Kaneko,Li Q,et al. Long-term effects of high dietary fiber intake on glucose tolerance and lipid metabolism in GK rats :comparison among barley,rice,and cornstarch. Metabolism,2003,52(9) :1206-1210
22.Anwarul Haque M,Shams-Ud-Din M.Haque A. The effect of aqueous extracted wheat bran on baking quality of biscuit. International Journal of Food Science&Technology,2002, 37(4):453-462
23.陈霞,杨香久.豆渣的综合开发利用[J].食品研究与开发,2006(9):43-44
24.沙金华.豌豆分离蛋白的制备、性质及应用研究[D]:[硕士学位论文]江苏:江南大学,2009:3-7
25.李铁军,印遇龙,黄瑞林,等.日粮纤维对猪回肠微生物氮及微生物氨基酸流量的影响[J].畜牧兽医学报,2003,34(3):239-244
26.刘云.用本地资源豌豆皮、麦秸栽培平菇技术[J].生物学通报,2009,44(3):55-56
27.刘伟伟,尹芳,张无敌.豌豆壳沼气发酵潜力的研究[J].云南师范大学学报,2005,25(6):28-29
28.B.Dave Oomah, Francois Caspar, Linda J.Malcolmson et al. Phenolics and antioxidant activity of lentil and pea hulls. Food Research International[J].2011,1(44):436-441
29.C.M.G.C Renard, R.M Weightman, J.F Thibault. The xylose-rich pectins from pea hulls. International Journal of Biological Macromolecules [J].1997,1-2(21):155-162
30.R.M.Weightman, C.M.G.C. Renard, J.-F.Thibautl.Structure and properties of the polysaccharides from pea hulls. Part 1: Chemical extraction and fractionation of the polysaccharides.Carbohydrate Polymers[J],1994,2(24):139-148
31.J.M.Fang, J.M.Lawther,etc. Fractionation and characterization of polysaccharides from abaca fiber.Carbohydrate Polymer.1998.315-31
32.Kainuma S,Nakamura N,Yamamoto M.Method of preparing water-soluble dietary fiber. US Patent,5622738.1997-04-22
33.姜竹茂,陈新美,廖静.从豆渣种制备可溶性膳食纤维的研究[J].中国粮油学报,2001,16(3):52-55
34.SHIBA K,YAMADA H,HARA H et al.Preparation and Effect of cell-wall hydrolysate from wheat bran on mixing properties of dough.Cereal chemistry,1994,71(3):279-282
35.孙云霞.豆渣中水溶性膳食纤维提取方法的研究[J].食品研究与开发,2003,24(3):34-35
36.黄晓青,翟伟箐,王燕等.采用酶法从豆渣种制备2种膳食纤维.2004,30(1):25-28
37.张洪,米乳饮料的研制[J].食品工业,2000(5):13-15
38.Mitchell Cheryl R, Mitchell Pat R.et al. Nutritional rice milk production [patent] US4744992,1988
39.Nam Sung-Hee,Seo Jang-Ho et al. Rice based beverage product and process for making the same [patent] US6265001,2001
40.熊素敏,左秀凤,朱永义.稻壳中纤维素、半纤维素和木质素的测定[J].粮食与饲料工业,2005,8:40-41
41.黄海,杨瑞金,王璋.低聚木糖的脱色工艺[J].无锡轻工大学学报,2002,21(2):125-129
42.余斌.小麦B淀粉糖制备技术的研究及其麦芽糊精在蛋糕中的应用[D]:[硕士学位论文]江苏:江南大学,2008:20-22
43.陶颜娟.小麦麸皮膳食纤维的改性及应用研究[D]:[硕士学位论文]江苏:江南大学,2008:12-14
44.Shimada K,Fijikawa K,Yahara K,et al.Antioxidative properties of xanthan on the antioxidation of soybean oil in cyclodextrin. Journal of Agriculture and Food Chemistry,1992, 40:945-948
45.Marklund S,Marklund.Involvement of the superoxide anion radical in the antoxidation of pyrogallol and a convenient assay for superoxide dismutase. Europe Journal of Biochemistry, 1974,47: 469-474
46.Oyaizu M.Studies on products of browning reaction:antioxidative activities of products of browning reaction prepared from glucosa mine.Japanese Journal of Nutrition,1986,44: 307-315
47.Carter P.Spectrophotometric deter mination of serum iron at the submicrogram level with a new reagent (ferozine).Analytical Biochemistry,1971,40:450-458
48.Sangnark A,Noomhorm A.Effect of particle sizes on functional properties of dietary fiber prepared from sugarcane bagasse[J].Food Chemistry,2003(80):221-229
49.胡国华,黄绍华.米糠膳食纤维对胆酸钠吸附作用的研究[J].中国食品添加剂,2001(2):10-12
50.欧仕益,郑妍,刘子力,等.酵解和酶解麦麸吸附脂肪和胆固醇的研究[J].食品科技,2005(1):91-93
51.林德荣.可溶性膳食纤维提取、理化性质及其生理功能的研究[D]:[硕士学位论文]江西:南昌大学,2008:62-64
52.KakoM,Minural T,Nishyama Y,etal.Hypoglycemic effect of the rhizome sof polygala senega in normal and Diabetic mice and its main component.Planta Med,1996,62(5):440-443
53. Kabe A,Miura T.Hypoglycemic activity of polygon ati-Rhizome in normal and diabetic mice.Biolp harm Bull,1993,16(11):1118-1120
54.侯会池,黎介寿.膳食纤维的生理功能及临床作用[J].食用临床医学杂志,2004,8(5):12-15
55.徐淑云,卞如镰,陈修.药理实验方法学[M]第三版,北京:人民卫生出版社,2002,1269-1280
56.涂清荣.米乳饮料的制备及其稳定性的研究[D]:[硕士学位论文]江苏:江南大学,2005:30-38
57.徐广超.豆渣可溶性膳食纤维的制备及功能性的研究[D]:[硕士学位论文]江苏:江南大学,2005:15-16
58.肖琼.超声波辅助植物纤维原料酶水解的研究[D]:[硕士学位论文]江苏:南京林业大学,2006:38-39
59. A talla R H,A garwal U P.Science,1985,227:636-638
60.张惟杰.糖复合物生化技术研究.杭州:浙江大学出版社,1999
61.Nandini C,Salimath P V.Structural features of arabinoxylans from sorghum having good roti-making quality.Food Chemistry,2001.,74:417-422
62.Dobhl M P,Hasan A M,Sharma M C,et al.Ferulic acid ester from Plumeria bicolor. Food Chemistry,1999,51:319-321
63.朱美静.猴头菌多糖的提取及理化性质的研究[D]:[硕士学位论文]江苏:江南大学,2006:39-44
64.梁建忠,顾苒,等.大豆水溶性多糖的黏度性质及其应用研究[J].华北农学报,2007,22(z2):143-145
65.陈宗其.胶体化学[M].北京:高等教育出版社,1985
66.华聘聘,刘忠萍.可溶性大豆膳食纤维的单糖组分分析及物性测定[J].中国油脂,2004(29):23-27
67.Kehrer J P.The Haber-Weiss reaction and mechanisms of toxicity. Toxicology, 2000,149:43-50
68.Pulido R,Bravo L,Saura-Calixto F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay.Journal of Agricultural and Food Chemistry, 2000, 48(8):33396-3402
69.荣建华,李小定.大豆多肽抗氧化研究效果[J].食品科学,2002,23(11):118-120
70.饶家麟,柯文庆.鲣鱼蒸煮液蛋白质水解物之抗氧化活性[J].台湾农业化学与食品科学,2001,39(5):363-369
71.Decker E A, Welch B. Role of ferritin as a lipid oxidation catalyst in muscle foo[J].Journal of Agricultural and food Chemistry,1990,30(3):674-677
72.Kehrer J P.The Haber-Weiss reaction and mechanisms of toxicity.Toxicology, 2000,149: 43-50
73.田向阳,李玉清,韩梅,等.北京市小学生与肥胖相关知识、态度、行为的现况调查[J].中国健康教育,2004,20(5):393-395
74.袁尔东,郑建仙.多功能大豆纤维降脂作用及其机理探讨[J].粮食与饲料工业,2000.(7):46-47
75.张钟,王德生.玉米膳食纤维对实验性高脂血症小鼠降脂作用的影响[J].中国粮油学报,2005,20(5):120-123
76.赵海雯.癞葡萄皂甙的分离纯化及降血糖功效研究[D]:[硕士学位论文]江苏:江南大学,2005:66-67
77.许曼音.糖尿病学[M].上海科学技术出版社,2003:35-28