不同年份蕲艾的理化效应差异研究
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摘要
艾草的应用在我国有着悠久的历史。中医认为艾叶苦燥辛散,能理气血、温经脉、逐寒湿、止冷痛,艾叶预防瘟疫已有几千年的历史,中草药可以就地取材,且现代医学的药理研究表明艾叶是一种广谱抗菌抗病毒的药物,它对多种病毒和细菌都有抑制和杀伤作用,对呼吸系统疾病有一定的防治作用。
灸法或称灸疗,是祖国医学中的重要组成部分,是我国古代劳动人民长期与疾病作斗争的一大经验总结,是一种防治疾病、强身健体的医疗方法。灸疗使用艾绒或其他药物放置体表的腧穴或疼痛处烧灼、温熨,借灸火的温和热力及药物作用,通过经络的传导,以温通经脉、调和气血、协调阴阳、扶正祛邪,达到治疗疾病、防病保健、养生美容之功效。灸法很早就被人们所重视,由于其安全性高、无毒副作用、养生保健,因此流传很广。现代医疗方法应用的推广是以疗效作为前提的,而疗效又是通过药性及作用量来获得的。本文从灸材入手,通过实验手段对艾叶药理特性进行分析,以期为艾灸的机制研究和提高艾灸疗法的有效性提供数据支持。
通过对不同年份蕲艾绒挥发油的成分进行测定,来检测艾灸疗法对于小鼠痛阈及疲劳恢复的影响,并观察温和灸下的人体体表温度变化,从温度角度探讨不同灸材影响灸效的意义。
实验一用气相色谱-质谱法研究不同年份艾绒(1年和5年)挥发油含量,比较不同年份艾绒挥发油的组分；
实验二将实验小鼠随机分为6组：空白对照组、1年艾灸组、5年艾组、挥发油A组(1年艾组)、挥发油B组(5年艾组)、阳性组,以观察不同处理方法对小鼠热板镇痛实验痛阈值的影响；
实验三将实验小鼠随机分为5组：空白对照组、1年艾灸组、5年艾组、挥发油A组(1年艾组)、挥发油B组(5年艾组),以观察不同处理方法对小鼠游泳实验中游泳时间的影响；
临床研究方法：按照随机分组的方法将90例受试者分成3组：1年艾组30例、3年艾组30例、5年艾组30例。分别测量3种不同年份(1年、3年、5年)温和灸施灸右侧肺俞穴位的体表温度,对施灸前、施灸过程中(2分钟、4分钟、6分钟、8分钟、10分钟)穴表面温度变化进行比较,并调查了解受试者在不同温和灸过程中的舒适感及接受度。
不同年份艾绒的挥发油化学成份分析：1年蕲艾和5年蕲艾的挥发油组分分析比较：1年艾中发现52种化学成分,5年艾中发现50种化学成分；1年艾中挥发油含量较5年艾多。
小鼠镇痛实验分析：干预前各组热痛阂值比较,差异无统计学意义(p>0.05)干预后,组间方差分析比较,差异有统计学意义(p<0.05),进行两两比较,与空白组比较,西药组、艾灸组以及挥发油组,差异有统计学意义(p<0.05)；与西药组比较,艾灸组以及挥发油组,差异有统计学意义(p<0.05)；与艾灸A组比较,艾灸B组及挥发油组,差异有统计学意义(p<0.05)；艾灸B组、挥发油A、B组间比较,差异无统计学意义(p＞0.05)。
小鼠抗疲劳试验分析：干预前各组小鼠力竭时间值比较,差异无统计学意义(p>0.05)干预后,组间方差分析比较,差异有统计学意义(p＜0.05),进行两两比较,与空白组比较,艾灸组及挥发油组,差异有统计学意义(p＜0.05)；艾灸A、B组比较,差异有统计学意义(p＜0.05)；挥发油A、B组比较,差异无统计学意义(p＞0.05)；艾灸A组与挥发油A组比较,差异有统计学意义(p＜0.05)；艾灸B组与挥发油B组比较,差异有统计学意义(p＜0.05)。
临床研究：三组受试者在年龄、性别构成方面比较均无显著性差异(p>0.05),提示三组的一般情况具有可比性。施灸前,各组体表温度无显著性差异(p>0.05)。与1年艾相比,3年艾除在施灸4分钟、8分钟有显著性差异(p<0.05),其他时间点无显著性差异(p>0.05)；与1年艾相比,5年艾在各个时间点都有显著性差异(p<0.05)。与3年艾相比,5年艾在各个时间点均有显著性差异(p<0.05)。以上三种艾条组内任两个时间点的比较均有显著性差异(p<0.05)。舒适度方面,三组间舒适度比较有显著性差异(p<0.05),1年艾组与3年艾组组间舒适度比较无显著性差异(p>0.05),1年艾组与5年艾组组间舒适度比较有显著性差异(p<0.05),3年艾组与5年艾组组间舒适度比较有显著性差异(p<0.05)。接受度方面,1年艾与3年艾相比无显著性差异(p>0.05),1年艾与5年艾相比有显著性差异(p<0.05),3年艾与5年艾相比有显著性差异(p<0.05)。
1.不同年份艾绒的挥发油化学成份分析：
结果显示,不同年份蕲艾绒挥发油组分基本相同,但其含量有较差别：年份越久、艾绒比例越高,易挥发成分的相对含量越少,难挥发成分含量越多。表明蕲艾叶及艾绒挥发油成分基本是安全的。陈年高比例艾绒挥发油以难挥发成分为主,如刺柏脑、石竹素、石竹烯等,这些难挥发物质可能就是艾灸时的有效成分。结果表明两种样品组分基本一致,但挥发油含量不同,5年比1年含量少；且挥发油成分中的难挥发性物质含量相对较多。
2.小鼠镇痛实验分析：
艾灸组与挥发油组均能显著增加实验小鼠的痛阈值,不同年份艾条灸疗,产生的镇痛作用有差异,5年艾优于1年艾；不同年份艾绒挥发油通过呼吸道产生的镇痛作用差异不明显；同一年份艾,艾条艾灸与艾绒挥发油的镇痛作用比较,1年艾挥发油优于艾灸,5年艾灸组与5年艾挥发油组的镇痛效果差异不明显。
3.小鼠抗疲劳试验分析：
艾灸组与挥发油组均能显著增加小鼠游泳力竭时间。不同年份艾条灸疗,产生的抗疲劳作用有差异,5年艾优于1年艾；不同年份艾绒挥发油通过呼吸道产生的抗疲劳作用差异不明显；同一年份艾,艾条艾灸与艾绒挥发油呼吸道的抗疲劳作用有差异,艾灸疗法优于艾绒挥发油。
4.临床实验部分：1年艾燃烧速度要快于3年艾和5年艾。穴位局部皮肤温升速度以1年艾最快,3年艾次之,5年艾最慢。5年艾火力柔和,渗透力强,患者舒适度和接受度都明显高于1年艾组和3年艾组。提示5年艾更容易被受试者接受可能因其艾绒含量较高,挥发油含量较少,所以火力较柔和,温度上升较缓慢,而温度缓慢上升则避免了因温度快速上升而造成的对皮肤的刺激,所以患者舒适度评分较高；当然也有可能跟艾叶里的难挥发性物质有关。
The application of Asiatic wormwood has a centuries-old history in China. Chinese medicine thinks that Asiatic wormwood can recuperate QI and blood, warm meridian and channel, drive out cold and dampness, and stop crymodynia. Asiatic wormwood has a long history in preventing pestilence. The pharmacology research of Modern medicine indicates that Asiatic wormwood is a broad spectrum antibiosis medicine. Asiatic wormwood can restrain various virus and germ, and can prevent and cure some respiratory system diseases.
The moxibustion or moxa treatment is an important part of national medicine. It is a rich experience of ancient Chinese Laboring people drawing from long period's battles against the disease. And it is a kind of treatment, which can prevent and cure disease and make people stronger and healthier. The moxibustion burns the acupoint or the pain-point through moxa or other medicines to warm meridian and channel, recuperate QI and blood, Coordinate yin and yang, and strengthen body resistance and eliminating evil. The moxibustion has the function of curing diseases, preventing diseases, keeping in good health and improving looks. People pay attention to the moxibustion for a long time.Because of it's high safety and functions of keeping in good health and improving looks, the moxibustion is spreaded widely. Now the curative effect is a prerequisite to the application and dissemination of treatment. The effect appears through natural capacity of medicine and proper amount of application.This subject starting from the moxibustion material through experiment method to analyze moxa pharmacological characteristics. By this way, we want to provide some data support to the mechanism of moxibustion study and improve the effectiveness of moxibustion therapy.
Objective:
Through the Artemisia Argyi of different years from Qichun volatile constituents, to detect the determination of moxibustion therapy pain threshold and fatigue in mice. And observe the influence of the human body surface temperature under mild moxibustion change from different perspectives, and explore different moxibustion material effect significance from the aspect of temperature.
Method:
Experiment 1:through the research different years of moxa wool (1-year and 5-year)volatile oil content by gas chromatography-mass spectrometry, comparing different years of moxa wool components.
Experiment 2:Laboratory mice divided into six groups as follows:control group, 1-year of Ai group, Naphtha group A,5-year of Ai group, Naphtha group B, positive group, and observe different hot plate processing methods of analgesic experiments in mice pain threshold value influence.
Experiment 3:Laboratory mice divided into five groups as follows:control group, 1-year of Ai, Naphtha group A, 5-year of Ai, Naphtha group B, and observe influence from different processing methods of mice swimming in the experiment.
Clinical Research:The 90 volunteers randomly devided into three groups as follow:one-year 30 cases of Ai, three-year 30 cases of Ai, five-year 30 cases of Ai.Acupoint surface temperatures of three different years of warming moxibustion (1-year,3-year,5-year) were measured in 90 volunteers, and then a comparison of surface temperature changes in right acupoint(Feishu) was made among pre-moxibustion, moxibustion-processing(2、4、6、8、10min). The sense of comfort and the acceptance of the moxibustion were surveyed after the experiment.
Results:
Different years of moxa wool the chemical composition of essential oil analysis:The volatile oil composition analysis comparison were identified by gas chromatography-mass spectrometry (GC-MS):1-year of Ai found 52 species chemical composition, 5-year of Ai found 50 kinds of chemical composition; 1-year of Ai elliott volatile oil content is in more than 5-year of Ai.
The mice of analgesia experiments analysis:Comparing with all groups before treatment, there was no significant difference(p>0.05). Comparing with the control group after treatment, positive group, moxibustion group and naphtha group all have significant differences (P<0.05); Comparing with the positive group, moxibustion group and naphtha group have significant differences (P<0.05); Comparing with the moxibustion group A, moxibustion group B and naphtha group have significant differences (P<0.05);naphtha group A compared naphtha group B with no significant difference (p>0.05); moxibustion group B, naphtha group A and naphtha group B all have no significant difference (p>0.05).
The mice of resist to fatigue test analysis:Comparing with all groups before treatment, there was no significant difference(p>0.05). Comparing with the control group after treatment, naphtha group and moxibustion group all have significant differences (p<0.05); moxibustion group A compared with moxibustion group B have significant differences (p<0.05); naphtha group A compared naphtha group B have no significant difference (p>0.05); moxibustion group A compared with naphtha group A have significant difference (p<0.05);moxibustion group B compared with naphtha group B have significant difference (p<0.05).
Comprehensive clinical observation:three groups of participants in age and sex showed no significant difference(p>0.05), suggesting that the general situation of the three groups were comparable. There was no significant difference among the three groups before moxibustion (p>0.05).There was significant difference between 1-year of Ai and 3-year of Ai at the point of 4 and 8(p<0.05).There was significant difference between 1-year of Ai and 5-year of Ai at any point (p<0.05). The difference in temperature between 3-year of Ai and 5-year of Ai was significant(p<0.05).The differences in comfort standards among the three groups were significant(p<0.05). There was no significant difference in comfort standards between 1-year of Ai and 3-year of Ai while there was significant difference between 1-year of Ai and 5-year of Ai. Compared with 3-year Ai,5-year Ai had significant difference (p<0.05). There was no significant difference in acceptance between 1-year of Ai and 3-year of Ai(p>0.05). There was significant difference between 1-year of Ai and 5-year of Ai(p<0.05). Compared with 3-year of Ai,5-year of Ai had significant difference.
Conclusion:
1.Analysis about essential oil of Different years moxa:the chemical composition was similar between 1-year of Ai and 5-year of Ai, but the contents were different。The flammable fraction in 1-year of Ai was more than that in 5-year of Ai. The involatile fractions were more in 5-year of Ai, and these fractions may be the valid element.
2. The mice of analgesia experiments analysis:Moxibustion group and control group were significantly higher volatile caused glacial acetic acid in mice pain threshold, but compared with the positive group,the effect was a bit poor; moxibustion group B was better than moxibustion group A to ascend the pain threshold of mice, volatile oils group B was better than volatile group A in ascending the pain threshold of mice. It suggested that the contents of involatile fractions may be the cause of ascending the pain threshold of mice.
3. The mice of resist to fatigue test analysis:Comparing with the control group, naphtha group and moxibustion group all had significant differences, and suggested naphtha and moxibustion could make tired mice get recovery rapidly; moxibustion group B compared with moxibustion group A had significant difference (p<0.05). It can be concluded that 5-year of Ai had fewer flammable fractions than 1-year of Ai, so the penetration 5-year of Ai was better than 1-year of Ai.
4. Clinical experiment:The burning speed of 1-year of Ai was the fastest of the three groups.1-year of Ai can raised the temperature of the point fastest. The comfort standards and acceptance standards of 5-year of Ai were higher than 1-year of Ai, and higher than 3-year of Ai, too. The volatile oil of 5-year of Ai was scarce, and the moxa was rich, so the temperature of 5-year of Ai raised gently, and the score of comfort standards was the highest; and maybe the involatile fractions resulted in the highest comfort standards of the subjucts.
灸法或称灸疗,是祖国医学中的重要组成部分,是我国古代劳动人民长期与疾病作斗争的一大经验总结,是一种防治疾病、强身健体的医疗方法。灸疗使用艾绒或其他药物放置体表的腧穴或疼痛处烧灼、温熨,借灸火的温和热力及药物作用,通过经络的传导,以温通经脉、调和气血、协调阴阳、扶正祛邪,达到治疗疾病、防病保健、养生美容之功效。灸法很早就被人们所重视,由于其安全性高、无毒副作用、养生保健,因此流传很广。现代医疗方法应用的推广是以疗效作为前提的,而疗效又是通过药性及作用量来获得的。本文从灸材入手,通过实验手段对艾叶药理特性进行分析,以期为艾灸的机制研究和提高艾灸疗法的有效性提供数据支持。
通过对不同年份蕲艾绒挥发油的成分进行测定,来检测艾灸疗法对于小鼠痛阈及疲劳恢复的影响,并观察温和灸下的人体体表温度变化,从温度角度探讨不同灸材影响灸效的意义。
实验一用气相色谱-质谱法研究不同年份艾绒(1年和5年)挥发油含量,比较不同年份艾绒挥发油的组分；
实验二将实验小鼠随机分为6组：空白对照组、1年艾灸组、5年艾组、挥发油A组(1年艾组)、挥发油B组(5年艾组)、阳性组,以观察不同处理方法对小鼠热板镇痛实验痛阈值的影响；
实验三将实验小鼠随机分为5组：空白对照组、1年艾灸组、5年艾组、挥发油A组(1年艾组)、挥发油B组(5年艾组),以观察不同处理方法对小鼠游泳实验中游泳时间的影响；
临床研究方法：按照随机分组的方法将90例受试者分成3组：1年艾组30例、3年艾组30例、5年艾组30例。分别测量3种不同年份(1年、3年、5年)温和灸施灸右侧肺俞穴位的体表温度,对施灸前、施灸过程中(2分钟、4分钟、6分钟、8分钟、10分钟)穴表面温度变化进行比较,并调查了解受试者在不同温和灸过程中的舒适感及接受度。
不同年份艾绒的挥发油化学成份分析：1年蕲艾和5年蕲艾的挥发油组分分析比较：1年艾中发现52种化学成分,5年艾中发现50种化学成分；1年艾中挥发油含量较5年艾多。
小鼠镇痛实验分析：干预前各组热痛阂值比较,差异无统计学意义(p>0.05)干预后,组间方差分析比较,差异有统计学意义(p<0.05),进行两两比较,与空白组比较,西药组、艾灸组以及挥发油组,差异有统计学意义(p<0.05)；与西药组比较,艾灸组以及挥发油组,差异有统计学意义(p<0.05)；与艾灸A组比较,艾灸B组及挥发油组,差异有统计学意义(p<0.05)；艾灸B组、挥发油A、B组间比较,差异无统计学意义(p＞0.05)。
小鼠抗疲劳试验分析：干预前各组小鼠力竭时间值比较,差异无统计学意义(p>0.05)干预后,组间方差分析比较,差异有统计学意义(p＜0.05),进行两两比较,与空白组比较,艾灸组及挥发油组,差异有统计学意义(p＜0.05)；艾灸A、B组比较,差异有统计学意义(p＜0.05)；挥发油A、B组比较,差异无统计学意义(p＞0.05)；艾灸A组与挥发油A组比较,差异有统计学意义(p＜0.05)；艾灸B组与挥发油B组比较,差异有统计学意义(p＜0.05)。
临床研究：三组受试者在年龄、性别构成方面比较均无显著性差异(p>0.05),提示三组的一般情况具有可比性。施灸前,各组体表温度无显著性差异(p>0.05)。与1年艾相比,3年艾除在施灸4分钟、8分钟有显著性差异(p<0.05),其他时间点无显著性差异(p>0.05)；与1年艾相比,5年艾在各个时间点都有显著性差异(p<0.05)。与3年艾相比,5年艾在各个时间点均有显著性差异(p<0.05)。以上三种艾条组内任两个时间点的比较均有显著性差异(p<0.05)。舒适度方面,三组间舒适度比较有显著性差异(p<0.05),1年艾组与3年艾组组间舒适度比较无显著性差异(p>0.05),1年艾组与5年艾组组间舒适度比较有显著性差异(p<0.05),3年艾组与5年艾组组间舒适度比较有显著性差异(p<0.05)。接受度方面,1年艾与3年艾相比无显著性差异(p>0.05),1年艾与5年艾相比有显著性差异(p<0.05),3年艾与5年艾相比有显著性差异(p<0.05)。
1.不同年份艾绒的挥发油化学成份分析：
结果显示,不同年份蕲艾绒挥发油组分基本相同,但其含量有较差别：年份越久、艾绒比例越高,易挥发成分的相对含量越少,难挥发成分含量越多。表明蕲艾叶及艾绒挥发油成分基本是安全的。陈年高比例艾绒挥发油以难挥发成分为主,如刺柏脑、石竹素、石竹烯等,这些难挥发物质可能就是艾灸时的有效成分。结果表明两种样品组分基本一致,但挥发油含量不同,5年比1年含量少；且挥发油成分中的难挥发性物质含量相对较多。
2.小鼠镇痛实验分析：
艾灸组与挥发油组均能显著增加实验小鼠的痛阈值,不同年份艾条灸疗,产生的镇痛作用有差异,5年艾优于1年艾；不同年份艾绒挥发油通过呼吸道产生的镇痛作用差异不明显；同一年份艾,艾条艾灸与艾绒挥发油的镇痛作用比较,1年艾挥发油优于艾灸,5年艾灸组与5年艾挥发油组的镇痛效果差异不明显。
3.小鼠抗疲劳试验分析：
艾灸组与挥发油组均能显著增加小鼠游泳力竭时间。不同年份艾条灸疗,产生的抗疲劳作用有差异,5年艾优于1年艾；不同年份艾绒挥发油通过呼吸道产生的抗疲劳作用差异不明显；同一年份艾,艾条艾灸与艾绒挥发油呼吸道的抗疲劳作用有差异,艾灸疗法优于艾绒挥发油。
4.临床实验部分：1年艾燃烧速度要快于3年艾和5年艾。穴位局部皮肤温升速度以1年艾最快,3年艾次之,5年艾最慢。5年艾火力柔和,渗透力强,患者舒适度和接受度都明显高于1年艾组和3年艾组。提示5年艾更容易被受试者接受可能因其艾绒含量较高,挥发油含量较少,所以火力较柔和,温度上升较缓慢,而温度缓慢上升则避免了因温度快速上升而造成的对皮肤的刺激,所以患者舒适度评分较高；当然也有可能跟艾叶里的难挥发性物质有关。
The application of Asiatic wormwood has a centuries-old history in China. Chinese medicine thinks that Asiatic wormwood can recuperate QI and blood, warm meridian and channel, drive out cold and dampness, and stop crymodynia. Asiatic wormwood has a long history in preventing pestilence. The pharmacology research of Modern medicine indicates that Asiatic wormwood is a broad spectrum antibiosis medicine. Asiatic wormwood can restrain various virus and germ, and can prevent and cure some respiratory system diseases.
The moxibustion or moxa treatment is an important part of national medicine. It is a rich experience of ancient Chinese Laboring people drawing from long period's battles against the disease. And it is a kind of treatment, which can prevent and cure disease and make people stronger and healthier. The moxibustion burns the acupoint or the pain-point through moxa or other medicines to warm meridian and channel, recuperate QI and blood, Coordinate yin and yang, and strengthen body resistance and eliminating evil. The moxibustion has the function of curing diseases, preventing diseases, keeping in good health and improving looks. People pay attention to the moxibustion for a long time.Because of it's high safety and functions of keeping in good health and improving looks, the moxibustion is spreaded widely. Now the curative effect is a prerequisite to the application and dissemination of treatment. The effect appears through natural capacity of medicine and proper amount of application.This subject starting from the moxibustion material through experiment method to analyze moxa pharmacological characteristics. By this way, we want to provide some data support to the mechanism of moxibustion study and improve the effectiveness of moxibustion therapy.
Objective:
Through the Artemisia Argyi of different years from Qichun volatile constituents, to detect the determination of moxibustion therapy pain threshold and fatigue in mice. And observe the influence of the human body surface temperature under mild moxibustion change from different perspectives, and explore different moxibustion material effect significance from the aspect of temperature.
Method:
Experiment 1:through the research different years of moxa wool (1-year and 5-year)volatile oil content by gas chromatography-mass spectrometry, comparing different years of moxa wool components.
Experiment 2:Laboratory mice divided into six groups as follows:control group, 1-year of Ai group, Naphtha group A,5-year of Ai group, Naphtha group B, positive group, and observe different hot plate processing methods of analgesic experiments in mice pain threshold value influence.
Experiment 3:Laboratory mice divided into five groups as follows:control group, 1-year of Ai, Naphtha group A, 5-year of Ai, Naphtha group B, and observe influence from different processing methods of mice swimming in the experiment.
Clinical Research:The 90 volunteers randomly devided into three groups as follow:one-year 30 cases of Ai, three-year 30 cases of Ai, five-year 30 cases of Ai.Acupoint surface temperatures of three different years of warming moxibustion (1-year,3-year,5-year) were measured in 90 volunteers, and then a comparison of surface temperature changes in right acupoint(Feishu) was made among pre-moxibustion, moxibustion-processing(2、4、6、8、10min). The sense of comfort and the acceptance of the moxibustion were surveyed after the experiment.
Results:
Different years of moxa wool the chemical composition of essential oil analysis:The volatile oil composition analysis comparison were identified by gas chromatography-mass spectrometry (GC-MS):1-year of Ai found 52 species chemical composition, 5-year of Ai found 50 kinds of chemical composition; 1-year of Ai elliott volatile oil content is in more than 5-year of Ai.
The mice of analgesia experiments analysis:Comparing with all groups before treatment, there was no significant difference(p>0.05). Comparing with the control group after treatment, positive group, moxibustion group and naphtha group all have significant differences (P<0.05); Comparing with the positive group, moxibustion group and naphtha group have significant differences (P<0.05); Comparing with the moxibustion group A, moxibustion group B and naphtha group have significant differences (P<0.05);naphtha group A compared naphtha group B with no significant difference (p>0.05); moxibustion group B, naphtha group A and naphtha group B all have no significant difference (p>0.05).
The mice of resist to fatigue test analysis:Comparing with all groups before treatment, there was no significant difference(p>0.05). Comparing with the control group after treatment, naphtha group and moxibustion group all have significant differences (p<0.05); moxibustion group A compared with moxibustion group B have significant differences (p<0.05); naphtha group A compared naphtha group B have no significant difference (p>0.05); moxibustion group A compared with naphtha group A have significant difference (p<0.05);moxibustion group B compared with naphtha group B have significant difference (p<0.05).
Comprehensive clinical observation:three groups of participants in age and sex showed no significant difference(p>0.05), suggesting that the general situation of the three groups were comparable. There was no significant difference among the three groups before moxibustion (p>0.05).There was significant difference between 1-year of Ai and 3-year of Ai at the point of 4 and 8(p<0.05).There was significant difference between 1-year of Ai and 5-year of Ai at any point (p<0.05). The difference in temperature between 3-year of Ai and 5-year of Ai was significant(p<0.05).The differences in comfort standards among the three groups were significant(p<0.05). There was no significant difference in comfort standards between 1-year of Ai and 3-year of Ai while there was significant difference between 1-year of Ai and 5-year of Ai. Compared with 3-year Ai,5-year Ai had significant difference (p<0.05). There was no significant difference in acceptance between 1-year of Ai and 3-year of Ai(p>0.05). There was significant difference between 1-year of Ai and 5-year of Ai(p<0.05). Compared with 3-year of Ai,5-year of Ai had significant difference.
Conclusion:
1.Analysis about essential oil of Different years moxa:the chemical composition was similar between 1-year of Ai and 5-year of Ai, but the contents were different。The flammable fraction in 1-year of Ai was more than that in 5-year of Ai. The involatile fractions were more in 5-year of Ai, and these fractions may be the valid element.
2. The mice of analgesia experiments analysis:Moxibustion group and control group were significantly higher volatile caused glacial acetic acid in mice pain threshold, but compared with the positive group,the effect was a bit poor; moxibustion group B was better than moxibustion group A to ascend the pain threshold of mice, volatile oils group B was better than volatile group A in ascending the pain threshold of mice. It suggested that the contents of involatile fractions may be the cause of ascending the pain threshold of mice.
3. The mice of resist to fatigue test analysis:Comparing with the control group, naphtha group and moxibustion group all had significant differences, and suggested naphtha and moxibustion could make tired mice get recovery rapidly; moxibustion group B compared with moxibustion group A had significant difference (p<0.05). It can be concluded that 5-year of Ai had fewer flammable fractions than 1-year of Ai, so the penetration 5-year of Ai was better than 1-year of Ai.
4. Clinical experiment:The burning speed of 1-year of Ai was the fastest of the three groups.1-year of Ai can raised the temperature of the point fastest. The comfort standards and acceptance standards of 5-year of Ai were higher than 1-year of Ai, and higher than 3-year of Ai, too. The volatile oil of 5-year of Ai was scarce, and the moxa was rich, so the temperature of 5-year of Ai raised gently, and the score of comfort standards was the highest; and maybe the involatile fractions resulted in the highest comfort standards of the subjucts.
引文
[1]陆寿康,杨华元,徐恒泽,等.刺法灸法学[J].北京：中国中医药出版社,2003,87-92.
[2]大西基代.艾燃烧生成物的自由基清除作用研究[J].国外医学*中医中药分册,1992,14(3)：60.
[3]江苏新医学院.中药大辞典(上册).上海：上海人民出版社,1997：359.
[4]邹菁,刘安昌,张平.活性离子水提取艾叶挥发油的研究[J].武汉化工学院学报,2006,28(4)：27-30.
[5]黄秋风译.艾的有机成分.国外医学·中医中药分册,1989;11(5)：47.
[6]王永林,刘拉平.艾叶挥发性成分固相微萃取GC-MS分析[J].西北药学杂志,2009,24,(5)：354-357.
[7]吉双,张予川,刁云鹏等.艾叶的化学成分[J].沈阳药科大学学报,2009,26(8)：617-619.
[8]刘屏荣译.关于隔物灸的研究.国外医学*中医中药分册,1989,11(5)：47.
[9]徐新建,宋海,韩玉琦,等.艾叶挥发油化学成分的气相色谱—质谱联用分析[J].时珍国医国药,2007,18(11)：2657-2658.
[10]王全杰,高龙.胶东沿海地区艾蒿精油成分的研究[J].烟台大学学报,2010,23(2)：105-110.
[11]姜平川,李嘉,梁家昌.广西产艾叶挥发油成分GC2MS研究[J].中国实验方剂学杂志,2009,15(12)：25-27.
[12]孟慧,许勇.沪产艾蒿鲜叶挥发油成分的GC2MS分析[J].药学实践杂志,2009,27(5)：362-364.
[13]兰美兵,余永莉,李啸红.贵州产艾叶挥发油的化学成分分析[J].药物分析杂志,2009,29(8)：1305-1308.
[14]朱江.日本产艾蒿与中国产艾蒿的异同[J].国外医学中医中药分册,1998,20(4)：24-25.
[15]何正有,张艳红,魏冬,等.湖北产鲜艾与陈艾挥发油的化学成分[J].中成药,2009,31(7)：1079-1082.
[16]靳然,于密密,赵百孝,等.不同年份蕲艾叶及不同比例艾绒化学成分研究[J].中国针灸,2010,30(5)：389-392.
[17]刘枫林,袁慧,许三荣,等.艾灸烟熏作用的抑菌效应[J].云南中医杂志,2003,4(5)：29-31.
[18]梅全喜,徐景远.艾烟的化学成分及药理作用研究进展[J].时珍国医国药.2003,14(8)：30-32.
[19]吕荧,叶春枚,高健芳,等.熏灸治疗外科感染性疾病575例[J].安徽中医学院学报,2003,7(4)：36-37.
[20]张文惠,汪国华,张嘉嘉.含雄黄药艾条烟气中砷及焦油含量的测定[J].中药材,1998,21(1)：23-25.
[21]Xiao-Hong Li, Cui Han, Lu-Fen Zhang, et al. Effect of moxibustion at point Dazhui on expression of brain-derived neurotrophic factor in chronic stress rats[J]. Chinese Journal of Clinical Rehabilitation,2004,8(1):194-196.
[22]吴巧凤,尹海燕,曾芳,等.艾灸补髓促进老年学习记减退大鼠海马神经发生[J].中国老年学杂志,2008,28(11)：2081-2083.
[23]李晓泓,韩毳,张露芳,等.艾灸大椎穴对慢性应激大鼠神经营养因子的影响[J].中医药学报,2002,30(6)：51-52.
[24]唐照亮,宋小鸽,陈全珠,等.艾灸抗炎免疫作用及对神经递质影响的实验研究[J].中国中医基础医学杂志,2000,6(9)：53-55.
[25]许建敏.胆硷能神经系统在艾灸调节小鼠免疫功能中的作用[J].针刺研究,1997,22(3)：166-167.
[26]杨志新,张晓峰,赵英侠,等.艾灸增强小鼠细胞免疫功能抗肿瘤作用的研究[J].中医药学刊,2002,20(1)：94-95.
[27]成拯,姜翼,陈凯.放化疗法配合艾灸神阙穴治疗晚期鼻咽癌42例近期疗效观察[J].新中医,2005,37(4)：58-59.
[28]唐照亮,宋小鸽,夏晓红,等.“热证可灸与贵灸”机制研究[J].安徽中医学院学报,2008,27(1)：29-32.
[29]邓柏颖,杨力强,罗敏然.化脓灸提高“体虚易感”患者免疫力的临床研究[J].四川中医,2005,23(1)：86-87.
[30]李晓泓,解秸萍,张露芬,等.预先艾灸对随后不同阶段更年期大鼠促性激素释放激素、卵泡刺激素和黄体生成素的干预作用[J],中国临床康复,2006,10(31)：99-101.
[31]白如鑫,李沛.温和灸对中老年部分雄激素缺乏综合征大鼠模型影响的实验研究[J].针刺研究,2007,32(4)：229-233,236.
[32]刘旭光,黄迪君,郝亮,等.艾灸对类风湿性关节炎大鼠下丘脑-垂体-肾上腺皮质轴的影响[J].中国中医基础医学杂志,2003,9(2)：137-139.
[33]李红,朱梅菊,高顺生.艾灸足三里穴对运动小鼠内分泌功能的影响[J].体育学刊,2003,10(6)：59-60.
[34]张周良,李斌,刘树林,等.艾灸对血液流变性影响的研究[J].中国血液流变学杂志,2004,14(4)：554-555.
[35]冯起国,裴景春,马铁明,等.艾灸内关对家兔动脉粥样硬化作用的研究[J].辽宁 中医杂志,2002,29(1)：48-49.
[36]吴中朝,王玲玲,刘跃光,等.艾灸对老年人血液载脂蛋白研究[J].中国针灸,1998,5：261-262.
[37]李强强.神阙穴治疗腹泻的体会[J].中国社区医师(综合版),2004,6(2)：461.
[38]易受乡,彭艳,常小荣,等.艾灸预处理对应激性溃疡大鼠胃黏膜增殖修复的影响[J].世界中西医结合杂志,2007,2(1)：21-24.
[39]杨丹红,江庆淇,许文波.“神阙”穴隔药灸对荷瘤化疗大鼠胃粘膜保护作用的研究[J].中国针灸,1999(8)：483-485.
[40]熊静宇,肖国强,卢艳梅.艾灸预处理对大鼠离心运动后骨骼肌组织保护作用的研究[J].山东体育学院学报,2010,26(10)：56-61.
[41]冯起国,肖凯,林立全等.艾灸对脾虚大鼠骨骼肌细胞线粒体酶活性的影响[J].辽宁中医杂志2000,27(7)：315-316.
[42]吴明学.艾灸治疗功能性遗尿症30例临床观察[J].针灸临床杂志,2004,20(11)：38.
[43]杨运宽,荣海波,陈洪沛等.艾灸对糖尿病性勃起功能障碍大鼠阴茎勃起功能及NO-cGMP通路的影响[J].中国针灸,2007,27(5)：353-356.
[44]周楣声.《灸绳》上册[M].青岛：青岛出版社,2006,66.
[45]邵永红.艾条灸治压疮94例及护理.针灸临床杂志,1999,15(5)：38.
[46]西谷郁子.全日本针灸学会杂志,1984,34(1)：66.
[47]施茵,刘慧荣,李双,等.艾灸治疗溃疡性结肠炎的临床研[J].中国现代临床医学,2005,4(7)：43-44.
[48]易受乡,林亚平,吴芳,等.神阙穴隔药饼灸、单纯灸及灯光灸对胃黏膜损伤保护作用的对比研究[J].中国中医药信息杂志,2005,12(10)：30-32.
[49]吴焕淦等.灸法的继承与创新[J],上海针灸杂志2007,26(12)：39-41.
[50]林文注,王佩.实验针灸学[M].上海：上海科学技术出版社,1994,67.
[51]陈云飞,杨文佳,马晓芃,等.不同时段艾灸血清中内生性蛋白组分双向电泳的实验研究[J].上海中医药大学学报,2007,21(2)：57-60.
[52]顾一煌,金宏柱,吴云川,等.不同艾灸量对运动后血清肌酸激酶影响的实验研究[J].南京中医药大学学报,2006,22(6)：373-375.
[53]顾一煌,金宏柱,吴云川,等.不同的艾灸量对运动后血乳酸影响的观察[J].辽宁中医杂志,2007,34(11)：1639-1640.
[54]尚秀葵.长时间温和灸治疗胃肠病临证举要.上海针灸杂志,2004,23(3)：22-23.
[55]桂金水.不同灸法对人体免疫功能的影响[J].上海针灸杂志,1983,(4)：21.
[56]楼百层.灸治肺结核病的临床研究[J].上海中医药杂志1964,(12)：27.
[57]赵天平,周爽,秦秀娣.不同灸法对溃疡性结肠炎大鼠结肠黏膜黏蛋白调节作用 的研究[J].中华中医药学刊,2010,28(8)：1590-1594.
[58]中国药典[S].一部.2000：67-68.
[59]周峰,秦路平,连桂芳,等.艾叶的化学成分、生物活性和植物资源[J].药学实践杂志,2000,2：96.
[60]朱亮锋,陆碧瑶,罗友娇.艾蒿和蕲艾精油化学成分的研究[J].云南植物研究,1985,7(4)：443.
[61]顾静文,刘立鼎,陈京达,等.艾蒿和野艾蒿精油的化学成分[J].江西科学,1998,16(4)：273.
[62]潘炯光,徐植灵,古力,等.艾叶挥发油的化学研究[J].中国中药杂志,1992,17(12)：741.
[63]刘国声.艾叶挥发油成分的研究[J].中草药,1990,21(9)：8.
[64]Seo J.艾蒿中黄酮的抗肿瘤活性[J].国外医学·中医中药分册,2005,27(1)：49.
[65]Tan Ten-xiang, Jia Zhong-jian. Eudesmanolides and other constituents from Artemisia argyi[J]. Plant Medica,1992,58(4):370.
[66]叶春枚,呈荧,高建芳.艾熏治愈54例手指骨髓炎临床观察与实验研究[J].上海针灸,1988(2)：7.
[67]林启寿.中草药成分化学[M].北京：科学技术出版社,1977：450-600.
[68]防治慢性气管炎艾叶油研究协作组.艾叶油及其有效成分的药理研究[J].医药业,1977,(11)：5.
[69]阴健、郭立弓.中药现代研究与临床应用[M].北京：学苑出版社,1993：319.
[70]文福姬,俞庆善,阉民燮.艾叶精油化学成分研究[J].香料香精化妆品,2007(3)：21-23.
[71]吴怀恩,李耀华,韦志英,等.广西产五月艾、细叶艾与艾叶挥发油的比较研究[J].药物研究,2008,5(35)：23-26.
[72]骆和生主编中药方剂的药理与临床研究进展[J].广州：华南理工大学出版社,1991：197.
[73]黄学红,谢元德,朱婉萍,等.艾叶油治疗慢性支气管炎的实验研究[J].浙江中医杂志,2006,41(12)：734-735.
[74]张燕.北艾蒿挥发油成分研究[J].广西植物,2006,26(1)：110-112.
[75]刘雪梅.浅谈艾灸抗癌[J].赤峰学院学报,2010,26(10)：54-55.
[76]张袁森,张琳,倪娜,等.艾叶的体外凝血作用实验研究[J].天津中医药,2010,27(2)：156-157.
[77]詹忠根.野艾蒿黄酮对活性氧自由基的清除作用及其脂肪酸成分的GC-MS分析[J].食品科技,2008,33(11)：177-179.
[78]张喜云,孟庆安.零陵香等药材挥发油含量的测定[J].武警医学院学报,1999,8(4)：236-237.
[79]江丹,易筠,杨梅,等.不同品种艾叶挥发油的化学成分分析[J].中国医药生物技术,2009,4(5)：339-344.
[80]何正有,张艳红,魏冬,等.三种不同提取方法制备的艾叶挥发油化学成分分析[J].中国医药生物技术,2008,3(4)：284-288.
[81]将涵,侯安继,项志学,等.蕲艾挥发油的抗炎、抗过敏和镇痛作用[J].医学新知杂志,2005,15(2)：36-39.
[82]刘乡.电针镇痛穴位特异性和广泛性的研究[J].针刺研究,1997,22(12)：66-70.
[83]杨锡让.实用运动生理学[M].北京体育大学出版社,2003,8(2)：402-403.
[84]田野.运动生理学高级教程[M].高等教育出版社,2003,8：462-464.
[85]Banister, E. W. Exereise induce hyper ammone mia[J]. Int. J. Sport sMed, 1990, (11):5129.
[86]Davis, J. M., etal. Possible mechanism so feen tralnervous system fatigue during exercise[J]. Med. Sei. SPortsExer,1997,29(1):45.
[87]郑陆,沈东颖,高丽,等.运动与氨代谢关系的研究进展[J].山东体育学院学报,2004,20(1)：413.
[88]将涵,侯安继,项志学,等.蕲艾挥发油的抗疲劳作用研究[J].武汉大学学报,2005,26(3)：373-375.
[89]程珂,魏建子,沈雪勇.不同材质针灸针温针灸的温度特性研究[J].中华中医药学刊.2011,(04)：703-706.
[90]洪宗国.艾与蕲艾[J].中国医药学报,2002,17：222-225.
[91]胡世林.中国道地药材论丛(M).北京：北京中医古籍出版社,1997,23,105.
[92]梅全喜.不同产地艾叶燃烧放热量的比较[J],中药材,17(9)：46-47.
[93]洪宗国.艾与蕲艾的生药学研究与开发[J].中医药学刊,2003,21(8)：1356-1357.
[94]梅全喜,董普仁,王剑,等.不同产地艾叶中挥发油和微量元素含量的比较[J],中国中药杂志,1991,16(12)：718.
[95]蒋国庆.浅谈温和灸的灸量[J].上海针灸,2005,24(11)：34.
[96]陈日新,康明非.腧穴热敏化艾灸新疗法,北京：人民卫生出版社,2006,1.
[97]丁光宏,沈雪勇,褚君浩,等.中医灸与人体穴位红外辐射光谱特性研究.中国生物医学工程学报,2002：21(4)：356-360.
[98]洪文学,蔡建红,景军,等.传统中医艾灸疗法的热辐射光谱特性的研究.生物医学工程研究,2003：22(4)：27-30.
[99]王士超,王耀帅,王玲玲,等.温和灸不同灸量调节血脂的效应比较[J].辽宁中医药大学学报,2011,13(1)：33-34.
[100]张京英,刘农虞,杨兆民,等.灸法、灸量对家兔全血组胺含量的影响及其意义[J].中国针灸,1994,(6)：27-29.
[101]林静,王耀帅,王士超,等.不同灸量温和灸对高脂血症大鼠穴位局部温度影响的实验观察[J].中华中医药学刊,2011,29(2)：257-259.
[102]邢海辉,王玲玲,张建斌,等.温和灸不同时间参数对高胆固醇血症患者疗效的影响[J].中国针灸,2010,30(11)：937-940.
[2]大西基代.艾燃烧生成物的自由基清除作用研究[J].国外医学*中医中药分册,1992,14(3)：60.
[3]江苏新医学院.中药大辞典(上册).上海：上海人民出版社,1997：359.
[4]邹菁,刘安昌,张平.活性离子水提取艾叶挥发油的研究[J].武汉化工学院学报,2006,28(4)：27-30.
[5]黄秋风译.艾的有机成分.国外医学·中医中药分册,1989;11(5)：47.
[6]王永林,刘拉平.艾叶挥发性成分固相微萃取GC-MS分析[J].西北药学杂志,2009,24,(5)：354-357.
[7]吉双,张予川,刁云鹏等.艾叶的化学成分[J].沈阳药科大学学报,2009,26(8)：617-619.
[8]刘屏荣译.关于隔物灸的研究.国外医学*中医中药分册,1989,11(5)：47.
[9]徐新建,宋海,韩玉琦,等.艾叶挥发油化学成分的气相色谱—质谱联用分析[J].时珍国医国药,2007,18(11)：2657-2658.
[10]王全杰,高龙.胶东沿海地区艾蒿精油成分的研究[J].烟台大学学报,2010,23(2)：105-110.
[11]姜平川,李嘉,梁家昌.广西产艾叶挥发油成分GC2MS研究[J].中国实验方剂学杂志,2009,15(12)：25-27.
[12]孟慧,许勇.沪产艾蒿鲜叶挥发油成分的GC2MS分析[J].药学实践杂志,2009,27(5)：362-364.
[13]兰美兵,余永莉,李啸红.贵州产艾叶挥发油的化学成分分析[J].药物分析杂志,2009,29(8)：1305-1308.
[14]朱江.日本产艾蒿与中国产艾蒿的异同[J].国外医学中医中药分册,1998,20(4)：24-25.
[15]何正有,张艳红,魏冬,等.湖北产鲜艾与陈艾挥发油的化学成分[J].中成药,2009,31(7)：1079-1082.
[16]靳然,于密密,赵百孝,等.不同年份蕲艾叶及不同比例艾绒化学成分研究[J].中国针灸,2010,30(5)：389-392.
[17]刘枫林,袁慧,许三荣,等.艾灸烟熏作用的抑菌效应[J].云南中医杂志,2003,4(5)：29-31.
[18]梅全喜,徐景远.艾烟的化学成分及药理作用研究进展[J].时珍国医国药.2003,14(8)：30-32.
[19]吕荧,叶春枚,高健芳,等.熏灸治疗外科感染性疾病575例[J].安徽中医学院学报,2003,7(4)：36-37.
[20]张文惠,汪国华,张嘉嘉.含雄黄药艾条烟气中砷及焦油含量的测定[J].中药材,1998,21(1)：23-25.
[21]Xiao-Hong Li, Cui Han, Lu-Fen Zhang, et al. Effect of moxibustion at point Dazhui on expression of brain-derived neurotrophic factor in chronic stress rats[J]. Chinese Journal of Clinical Rehabilitation,2004,8(1):194-196.
[22]吴巧凤,尹海燕,曾芳,等.艾灸补髓促进老年学习记减退大鼠海马神经发生[J].中国老年学杂志,2008,28(11)：2081-2083.
[23]李晓泓,韩毳,张露芳,等.艾灸大椎穴对慢性应激大鼠神经营养因子的影响[J].中医药学报,2002,30(6)：51-52.
[24]唐照亮,宋小鸽,陈全珠,等.艾灸抗炎免疫作用及对神经递质影响的实验研究[J].中国中医基础医学杂志,2000,6(9)：53-55.
[25]许建敏.胆硷能神经系统在艾灸调节小鼠免疫功能中的作用[J].针刺研究,1997,22(3)：166-167.
[26]杨志新,张晓峰,赵英侠,等.艾灸增强小鼠细胞免疫功能抗肿瘤作用的研究[J].中医药学刊,2002,20(1)：94-95.
[27]成拯,姜翼,陈凯.放化疗法配合艾灸神阙穴治疗晚期鼻咽癌42例近期疗效观察[J].新中医,2005,37(4)：58-59.
[28]唐照亮,宋小鸽,夏晓红,等.“热证可灸与贵灸”机制研究[J].安徽中医学院学报,2008,27(1)：29-32.
[29]邓柏颖,杨力强,罗敏然.化脓灸提高“体虚易感”患者免疫力的临床研究[J].四川中医,2005,23(1)：86-87.
[30]李晓泓,解秸萍,张露芬,等.预先艾灸对随后不同阶段更年期大鼠促性激素释放激素、卵泡刺激素和黄体生成素的干预作用[J],中国临床康复,2006,10(31)：99-101.
[31]白如鑫,李沛.温和灸对中老年部分雄激素缺乏综合征大鼠模型影响的实验研究[J].针刺研究,2007,32(4)：229-233,236.
[32]刘旭光,黄迪君,郝亮,等.艾灸对类风湿性关节炎大鼠下丘脑-垂体-肾上腺皮质轴的影响[J].中国中医基础医学杂志,2003,9(2)：137-139.
[33]李红,朱梅菊,高顺生.艾灸足三里穴对运动小鼠内分泌功能的影响[J].体育学刊,2003,10(6)：59-60.
[34]张周良,李斌,刘树林,等.艾灸对血液流变性影响的研究[J].中国血液流变学杂志,2004,14(4)：554-555.
[35]冯起国,裴景春,马铁明,等.艾灸内关对家兔动脉粥样硬化作用的研究[J].辽宁 中医杂志,2002,29(1)：48-49.
[36]吴中朝,王玲玲,刘跃光,等.艾灸对老年人血液载脂蛋白研究[J].中国针灸,1998,5：261-262.
[37]李强强.神阙穴治疗腹泻的体会[J].中国社区医师(综合版),2004,6(2)：461.
[38]易受乡,彭艳,常小荣,等.艾灸预处理对应激性溃疡大鼠胃黏膜增殖修复的影响[J].世界中西医结合杂志,2007,2(1)：21-24.
[39]杨丹红,江庆淇,许文波.“神阙”穴隔药灸对荷瘤化疗大鼠胃粘膜保护作用的研究[J].中国针灸,1999(8)：483-485.
[40]熊静宇,肖国强,卢艳梅.艾灸预处理对大鼠离心运动后骨骼肌组织保护作用的研究[J].山东体育学院学报,2010,26(10)：56-61.
[41]冯起国,肖凯,林立全等.艾灸对脾虚大鼠骨骼肌细胞线粒体酶活性的影响[J].辽宁中医杂志2000,27(7)：315-316.
[42]吴明学.艾灸治疗功能性遗尿症30例临床观察[J].针灸临床杂志,2004,20(11)：38.
[43]杨运宽,荣海波,陈洪沛等.艾灸对糖尿病性勃起功能障碍大鼠阴茎勃起功能及NO-cGMP通路的影响[J].中国针灸,2007,27(5)：353-356.
[44]周楣声.《灸绳》上册[M].青岛：青岛出版社,2006,66.
[45]邵永红.艾条灸治压疮94例及护理.针灸临床杂志,1999,15(5)：38.
[46]西谷郁子.全日本针灸学会杂志,1984,34(1)：66.
[47]施茵,刘慧荣,李双,等.艾灸治疗溃疡性结肠炎的临床研[J].中国现代临床医学,2005,4(7)：43-44.
[48]易受乡,林亚平,吴芳,等.神阙穴隔药饼灸、单纯灸及灯光灸对胃黏膜损伤保护作用的对比研究[J].中国中医药信息杂志,2005,12(10)：30-32.
[49]吴焕淦等.灸法的继承与创新[J],上海针灸杂志2007,26(12)：39-41.
[50]林文注,王佩.实验针灸学[M].上海：上海科学技术出版社,1994,67.
[51]陈云飞,杨文佳,马晓芃,等.不同时段艾灸血清中内生性蛋白组分双向电泳的实验研究[J].上海中医药大学学报,2007,21(2)：57-60.
[52]顾一煌,金宏柱,吴云川,等.不同艾灸量对运动后血清肌酸激酶影响的实验研究[J].南京中医药大学学报,2006,22(6)：373-375.
[53]顾一煌,金宏柱,吴云川,等.不同的艾灸量对运动后血乳酸影响的观察[J].辽宁中医杂志,2007,34(11)：1639-1640.
[54]尚秀葵.长时间温和灸治疗胃肠病临证举要.上海针灸杂志,2004,23(3)：22-23.
[55]桂金水.不同灸法对人体免疫功能的影响[J].上海针灸杂志,1983,(4)：21.
[56]楼百层.灸治肺结核病的临床研究[J].上海中医药杂志1964,(12)：27.
[57]赵天平,周爽,秦秀娣.不同灸法对溃疡性结肠炎大鼠结肠黏膜黏蛋白调节作用 的研究[J].中华中医药学刊,2010,28(8)：1590-1594.
[58]中国药典[S].一部.2000：67-68.
[59]周峰,秦路平,连桂芳,等.艾叶的化学成分、生物活性和植物资源[J].药学实践杂志,2000,2：96.
[60]朱亮锋,陆碧瑶,罗友娇.艾蒿和蕲艾精油化学成分的研究[J].云南植物研究,1985,7(4)：443.
[61]顾静文,刘立鼎,陈京达,等.艾蒿和野艾蒿精油的化学成分[J].江西科学,1998,16(4)：273.
[62]潘炯光,徐植灵,古力,等.艾叶挥发油的化学研究[J].中国中药杂志,1992,17(12)：741.
[63]刘国声.艾叶挥发油成分的研究[J].中草药,1990,21(9)：8.
[64]Seo J.艾蒿中黄酮的抗肿瘤活性[J].国外医学·中医中药分册,2005,27(1)：49.
[65]Tan Ten-xiang, Jia Zhong-jian. Eudesmanolides and other constituents from Artemisia argyi[J]. Plant Medica,1992,58(4):370.
[66]叶春枚,呈荧,高建芳.艾熏治愈54例手指骨髓炎临床观察与实验研究[J].上海针灸,1988(2)：7.
[67]林启寿.中草药成分化学[M].北京：科学技术出版社,1977：450-600.
[68]防治慢性气管炎艾叶油研究协作组.艾叶油及其有效成分的药理研究[J].医药业,1977,(11)：5.
[69]阴健、郭立弓.中药现代研究与临床应用[M].北京：学苑出版社,1993：319.
[70]文福姬,俞庆善,阉民燮.艾叶精油化学成分研究[J].香料香精化妆品,2007(3)：21-23.
[71]吴怀恩,李耀华,韦志英,等.广西产五月艾、细叶艾与艾叶挥发油的比较研究[J].药物研究,2008,5(35)：23-26.
[72]骆和生主编中药方剂的药理与临床研究进展[J].广州：华南理工大学出版社,1991：197.
[73]黄学红,谢元德,朱婉萍,等.艾叶油治疗慢性支气管炎的实验研究[J].浙江中医杂志,2006,41(12)：734-735.
[74]张燕.北艾蒿挥发油成分研究[J].广西植物,2006,26(1)：110-112.
[75]刘雪梅.浅谈艾灸抗癌[J].赤峰学院学报,2010,26(10)：54-55.
[76]张袁森,张琳,倪娜,等.艾叶的体外凝血作用实验研究[J].天津中医药,2010,27(2)：156-157.
[77]詹忠根.野艾蒿黄酮对活性氧自由基的清除作用及其脂肪酸成分的GC-MS分析[J].食品科技,2008,33(11)：177-179.
[78]张喜云,孟庆安.零陵香等药材挥发油含量的测定[J].武警医学院学报,1999,8(4)：236-237.
[79]江丹,易筠,杨梅,等.不同品种艾叶挥发油的化学成分分析[J].中国医药生物技术,2009,4(5)：339-344.
[80]何正有,张艳红,魏冬,等.三种不同提取方法制备的艾叶挥发油化学成分分析[J].中国医药生物技术,2008,3(4)：284-288.
[81]将涵,侯安继,项志学,等.蕲艾挥发油的抗炎、抗过敏和镇痛作用[J].医学新知杂志,2005,15(2)：36-39.
[82]刘乡.电针镇痛穴位特异性和广泛性的研究[J].针刺研究,1997,22(12)：66-70.
[83]杨锡让.实用运动生理学[M].北京体育大学出版社,2003,8(2)：402-403.
[84]田野.运动生理学高级教程[M].高等教育出版社,2003,8：462-464.
[85]Banister, E. W. Exereise induce hyper ammone mia[J]. Int. J. Sport sMed, 1990, (11):5129.
[86]Davis, J. M., etal. Possible mechanism so feen tralnervous system fatigue during exercise[J]. Med. Sei. SPortsExer,1997,29(1):45.
[87]郑陆,沈东颖,高丽,等.运动与氨代谢关系的研究进展[J].山东体育学院学报,2004,20(1)：413.
[88]将涵,侯安继,项志学,等.蕲艾挥发油的抗疲劳作用研究[J].武汉大学学报,2005,26(3)：373-375.
[89]程珂,魏建子,沈雪勇.不同材质针灸针温针灸的温度特性研究[J].中华中医药学刊.2011,(04)：703-706.
[90]洪宗国.艾与蕲艾[J].中国医药学报,2002,17：222-225.
[91]胡世林.中国道地药材论丛(M).北京：北京中医古籍出版社,1997,23,105.
[92]梅全喜.不同产地艾叶燃烧放热量的比较[J],中药材,17(9)：46-47.
[93]洪宗国.艾与蕲艾的生药学研究与开发[J].中医药学刊,2003,21(8)：1356-1357.
[94]梅全喜,董普仁,王剑,等.不同产地艾叶中挥发油和微量元素含量的比较[J],中国中药杂志,1991,16(12)：718.
[95]蒋国庆.浅谈温和灸的灸量[J].上海针灸,2005,24(11)：34.
[96]陈日新,康明非.腧穴热敏化艾灸新疗法,北京：人民卫生出版社,2006,1.
[97]丁光宏,沈雪勇,褚君浩,等.中医灸与人体穴位红外辐射光谱特性研究.中国生物医学工程学报,2002：21(4)：356-360.
[98]洪文学,蔡建红,景军,等.传统中医艾灸疗法的热辐射光谱特性的研究.生物医学工程研究,2003：22(4)：27-30.
[99]王士超,王耀帅,王玲玲,等.温和灸不同灸量调节血脂的效应比较[J].辽宁中医药大学学报,2011,13(1)：33-34.
[100]张京英,刘农虞,杨兆民,等.灸法、灸量对家兔全血组胺含量的影响及其意义[J].中国针灸,1994,(6)：27-29.
[101]林静,王耀帅,王士超,等.不同灸量温和灸对高脂血症大鼠穴位局部温度影响的实验观察[J].中华中医药学刊,2011,29(2)：257-259.
[102]邢海辉,王玲玲,张建斌,等.温和灸不同时间参数对高胆固醇血症患者疗效的影响[J].中国针灸,2010,30(11)：937-940.