极北鲵消化道组织学及其内分泌细胞的研究
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摘要
组织学观察结果显示，极北鲵消化道胚后发育具有一定的时空顺序：消化道各器官是按照胃、肠、食管的顺序逐渐分化并不断完善的，消化道壁各层是按照粘膜上皮、环肌层、纵肌层、外膜、固有层、粘膜下层和粘膜肌层的发育顺序依次出现的。而极北鲵成体消化道均由粘膜层、粘膜下层、肌层和外膜等4层结构组成。
Grimelius法显示，嗜银细胞最早出现在41期胃粘膜上皮之间；肠中嗜银细胞首先出现在42期；而食管中嗜银细胞出现最晚，在43期被检测到。而嗜银细胞在其成体消化道各部分均有分布，其中以直肠部分布密度为最高，胃幽门和回肠次之，胃体处最低。并且其形态是多种多样的：有圆形、椭圆形、锥体形和梭形等。根据嗜银细胞的形态，认为其具有内分泌、外分泌和旁分泌的功能。
免疫组织化学方法显示，5-HT细胞分布于消化道全长，分布密度以食管部为最高，胃幽门部次之，十二指肠为最低。SS细胞除了贲门、空肠和直肠以外，其余各部位均有分布，且SS细胞在胃幽门部分布密度为最高，回肠部最低。Gas细胞仅在十二指肠和空肠中检测到。Glu细胞仅在贲门和胃体中观察到。SP细胞只在胃体部检测到，而PP细胞未检测到。极北鲵消化道内分泌细胞细胞的形态是多种多样的：圆形、椭圆形、锥体形和梭形。总体看来，内分泌细胞的分布密度和形态与其消化道的生理功能是相适应的。
Histology observation slowed that the digestive tract during postembryonicdevelopment of Salamandrella keyserlingii had a spatial and temporal order: thedigestive tract organs was according to stomach, intestine and esophagus of developingsequence appear.The digestive tract of the wall was according to the mucous epithelium,inner muscle, external muscle, adventitia, lamina propria, submucosa and muscularismucosa of developing sequence appear. The digestive tract of the adult ofSalamandrella keyserlingii had the four layers: mueosa, submueosa, muscular layer andadventitia.
The silver nitrate method of Grimelius showed that argentaffine cells were firstappeared between mucous epithelial cells in the stomach of Salamandrella keyserlingiiat stage41. The argentaffine cells were first observed in the intestine at stage42. Theargentaffine cells were appeared in the esophagus most night at stage43. But, theargyrophil cells could be found throughout the digestive tract of the adult ofSalamandrella keyserlingii. The distribution density was the highest in rectum, followedby pylorica part of stomach and ileum and the lowest in stomach. And the shape of theargentaffine cells were mainly round, ellipse, cone-shape and shuttle-shaped. Accordingto the morphology of the argentaffine cells, we suggest that argentaffine cells in thedigestive tract of Salamandrella keyserlingii have endocrine, exocrine and paracrinefunctions.
Immunhistochemical observation showed that5-HT positive cells distributethroughout the digestive tract of Salamandrella keyserlingii. The distribution densitywas the highest in esophagus, followed by pylorica part of stomach and the lowest induodinum. SS cells were observed throughout the digestive tract except in cardiac partof stomach, jejunum and rectum. The distribution density of SS cells was the highest inpylorica part of stomach and the lowest in ileum. Gas cells distributed mainly induodenum and jejunum. Glu cells distributed mainly in cardiac part of stomach and thebody of stomach. SP cells were observed only in the body of stomach. But, PP cellswere not detected.The shape of the endocrine cells in the digestive tract of Salamandrel- la keyserlingii were mainly round, ellipse, cone-shape and shuttle-shaped.Between thedistributive density of five kinds of endocrine cells are adapt with physiologicalfunction of digestive tract.
Grimelius法显示，嗜银细胞最早出现在41期胃粘膜上皮之间；肠中嗜银细胞首先出现在42期；而食管中嗜银细胞出现最晚，在43期被检测到。而嗜银细胞在其成体消化道各部分均有分布，其中以直肠部分布密度为最高，胃幽门和回肠次之，胃体处最低。并且其形态是多种多样的：有圆形、椭圆形、锥体形和梭形等。根据嗜银细胞的形态，认为其具有内分泌、外分泌和旁分泌的功能。
免疫组织化学方法显示，5-HT细胞分布于消化道全长，分布密度以食管部为最高，胃幽门部次之，十二指肠为最低。SS细胞除了贲门、空肠和直肠以外，其余各部位均有分布，且SS细胞在胃幽门部分布密度为最高，回肠部最低。Gas细胞仅在十二指肠和空肠中检测到。Glu细胞仅在贲门和胃体中观察到。SP细胞只在胃体部检测到，而PP细胞未检测到。极北鲵消化道内分泌细胞细胞的形态是多种多样的：圆形、椭圆形、锥体形和梭形。总体看来，内分泌细胞的分布密度和形态与其消化道的生理功能是相适应的。
Histology observation slowed that the digestive tract during postembryonicdevelopment of Salamandrella keyserlingii had a spatial and temporal order: thedigestive tract organs was according to stomach, intestine and esophagus of developingsequence appear.The digestive tract of the wall was according to the mucous epithelium,inner muscle, external muscle, adventitia, lamina propria, submucosa and muscularismucosa of developing sequence appear. The digestive tract of the adult ofSalamandrella keyserlingii had the four layers: mueosa, submueosa, muscular layer andadventitia.
The silver nitrate method of Grimelius showed that argentaffine cells were firstappeared between mucous epithelial cells in the stomach of Salamandrella keyserlingiiat stage41. The argentaffine cells were first observed in the intestine at stage42. Theargentaffine cells were appeared in the esophagus most night at stage43. But, theargyrophil cells could be found throughout the digestive tract of the adult ofSalamandrella keyserlingii. The distribution density was the highest in rectum, followedby pylorica part of stomach and ileum and the lowest in stomach. And the shape of theargentaffine cells were mainly round, ellipse, cone-shape and shuttle-shaped. Accordingto the morphology of the argentaffine cells, we suggest that argentaffine cells in thedigestive tract of Salamandrella keyserlingii have endocrine, exocrine and paracrinefunctions.
Immunhistochemical observation showed that5-HT positive cells distributethroughout the digestive tract of Salamandrella keyserlingii. The distribution densitywas the highest in esophagus, followed by pylorica part of stomach and the lowest induodinum. SS cells were observed throughout the digestive tract except in cardiac partof stomach, jejunum and rectum. The distribution density of SS cells was the highest inpylorica part of stomach and the lowest in ileum. Gas cells distributed mainly induodenum and jejunum. Glu cells distributed mainly in cardiac part of stomach and thebody of stomach. SP cells were observed only in the body of stomach. But, PP cellswere not detected.The shape of the endocrine cells in the digestive tract of Salamandrel- la keyserlingii were mainly round, ellipse, cone-shape and shuttle-shaped.Between thedistributive density of five kinds of endocrine cells are adapt with physiologicalfunction of digestive tract.
引文
1EK Balon. Terminology of Intervals in Fish Development[J]. J Fish Res Board Can,1975,32(9):1663~1670.
2刘美,李凤超,穆淑梅,等.半滑舌鳎胚胎发育组织学观察[J].四川动物.2008,27(5):737~739.
3Z Z Wan, T X Gao, X M Zhang, et al. Histological Study on the Digestive SystemDevelopment of Takifugu rubripes Larvae and Juvenile[J]. Journal of Ocean Univ-ersity of China,2006,1(5):39~44.
4林强,吕军仪,张彬.大海马消化系统胚后发育的形态学及组织学研究[J].带海洋学报.2007,26(6):46~51.
5关海红,匡友谊,徐伟,等.哲罗鱼消化系统器官发生发育的组织学观察[J].动物学杂志.2007,42(2):116~123.
6初庆柱,张健东,陈刚,等.军曹鱼消化系统胚后发育的组织学研究[J].水生态学杂志.2010,3(4):86~91.
7Hachero-Cruzado, JB Ortiz-Delgado, B Borrega, et al. Larval Organogenesis ofFlatfish Brill Scophthalmus rhombus L: Histological and Histochemical Aspects[J].Aquaeulture.2009,286(1-2):138~149.
8M P Garcia Hernandez, M T Lozano, M T Elbal, et al. Development of the Digesti-ve Tract of Sea Bass(Dicentrarchus labrax L).Light and Electron MicroscopicStudies[J]. Anat Embryol,2001,204(1):39~57.
9MI Sanchez-Amaya, JB Ortiz-Delgado, A Garcia-Lopez, et al. Larval Ontogeny ofRedbanded Seabream Pagrus aurigaValenciennes,1843with Special Reference tothe Digestive System. A Histological and Histochemical Approach[J].Aquaculture,2007,263(1-4):259~279.
10方华华,王波.星斑川鲽仔稚鱼消化系统发育的组织学研究[J].中国农学通报.2011,27(14):50~54.
11蒲德永,王志坚,赵海鹏,等.亚东鲑消化系统的形态学和组织学观察[J].四川动物.2006,25(4):825~828.
12J Rovira, A C Villaro, M E Bodegas, et al. Structural Study of the Frog Rana temp-oraria Larval Stomach[J].Tissue and Cell.1993,25(5):695~707.
13Atsuko Ishizuya-Oka. Epithelial Connective Tissue Cross Tale is Essential forRegene-ration of Intestinal Epithelium[J].J Nippon Med Sch.,2005.72(l):13~18.
14A Mary, Bonneville. Fine Structural Changes in the Intestinal Epithelium of theBull-frog during Metamorphosis[J].J Cell Biol,1963,18(3):579~597.
15M A Bowers. Histogenesis and Histolysis of the Intestinal Epithelium of Bufo lenti-ginosus [J]. Am J Anat,1909,9(1):263~279.
16王昱.花背蟾蜍消化道胚后发育的组织学观察[J].东北农业大学学报.2011,42(9):88~91.
17李宁,梁刚,刘婷婷.中国大鲵胃肠道胚后发育的解剖学与组织学观察[J].动物学杂志.2011,46(3):117~122.
18李倩,张育辉.中国林蛙消化管胚后发育的组织学观察[J].四川动物.2008,27(3):417~420.
19唐晓玲,刘振湘.不同发育期的牛蛙蝌蚪消化道组织学观察[J].湖北农学院学报.2001,21(4):332~334.
20王健鑫,金宇斌,王日昕.南移养殖中国林蛙早期胚胎发育的组织学研究[J].浙江农业学报.2008,20(4):245~249.
21华田苗,陈壁辉,王朝林.扬子鳄胚胎胃的组织发生[J].解剖学报.1996,27(2):221~224.
22李堃.扬子鳄胚胎消化系统内分泌细胞发生的免疫组化研究[D].芜湖:安徽师范大学.2002.
23赵宏霞,黄斌,赵万鹏.巴西彩龟消化道组织学的初步观察[J].四川动物.2010,29(3):437~439.
24华田苗,刘再群,陈士超,等.乌梢蛇胃上皮的组织学研究[J].动物学杂志.2001,36(6):7~10.
25甘文强,张晓雁,乔健,等.绿海龟消化道组织学观察[J].中国兽医杂志.2011,47(11):23~25.
26傅丽容,洪美玲,史海涛,等.中华花龟消化系统的组织学初步研究[J].四川动物.2007,26(2):270~273.
27李仲杰,杨友金,李继承.赤链华游蛇消化道粘膜上皮结构的扫描电镜观察[J].浙江大学学报(理学版).2003,30(4):453~456.
28刘忠虎,白晓辉,康相涛,等.父母代固始鸡十二指肠的发育形态学观察[J].中国兽医学报.2008,28(10):1181~1184.
29王政富,王丙云,卢玉葵,等.马冈鹅肠胚后发育的组织学观察[J].中国家禽学报.2003,7(1):28~31.
30黎观红,瞿明仁,晏向华,等.泰和鸡早期(0~12周龄)内脏器官生长发育规律的研究[J].经济动物学报.2005,9(1):35~38.
31王水亭.雏鸡消化道解剖与组织学特点[J].云南畜牧兽医.1999,1:10.
32刘玉堂,田秀华,于学伟,等.大鸨消化系统组织学观察[J].动物学杂志.2002,37(5):37~41.
33王丽萍，刘玉堂，肖向红，等.环颈雉消化系统组织形态学观察[J].动物学杂志.1994,29(3):26~28.
34张子慧,肖方,袁伟静,等.一雄性丹顶鹤消化系统组织形态学观察[J].动物学杂志.1999,34(3):39~41.
35王家乡,彭克美,杜安娜,等.非洲雏鸵鸟消化管的组织学观察[J].动物学杂志.2007,42(3):131~135.
36李铭,柳劲松.4种雀形目鸟消化道形态特征[J].动物学杂志.2008,43(l):116~121.
37张登荣,王新俭,王世英,等.兔生后胃、肝发育的组织学研究[J].河南职技师院学报.1991,19(4):33~36.
38张登荣,王世英,杜恒忠,等.兔生后舌和食管发育的组织学研究[J].中国兽医学报.1994,14(3):280~283.
39李树明,张方武,彭然,等.25日龄内哺乳仔猪消化系统发育研究[J].四川畜牧兽医学院学报.2002,16(3):6~10.
40王芳,余燕,牛红星.马铁菊头蝠消化系统的组织学观察[J].四川动物.2010,29(1):109~110.
41江剑平,林雪莹.赤腹松鼠(Callosciurus erythralus)消化道的解剖学和组织学研究[J].福建师范大学学报:自然科学版.2007,23(6):105~108.
42王平,曹悼,陈茂生.大熊猫Ailuropoda melanoleuca消化道的组织学观察[J].动物学研究.1982,3:27~28.
43刘进辉,谭理琦,王水莲,等.东北虎胃与小肠组织形态结构的研究[J].经济动物学报.2004,8(l):22~25.
44王志均,梅愈华,朱文玉.胃肠激素[M].北京:科学出版社.1985.1~10.
45W M Bayliss, E H Starling. The Mechanism of Pancreatic Secretion[J].J Phisiol,1902,28(5):325~353.
46T Fujita, S Kobayashi. Structure and Function of Gut Endocrine Cells. Int Rev CytolSuppl.1977,6:187~233.
47施志仪,陈晓武,顾一峰.牙鲆消化道组织学观察及内分泌细胞分布[J].中国水产科学.2006,13(5):851~855.
48M P Garcia Hernandez, M T Lozano, B Agulleiro. Ontogeny of Some EndocrineCells of the Digestive Tract in Sea Bass (Dicentrarchus labrax): An Immunocytoch-emical Study[J].Cell&Tissue Research,1994,277(2):373~383.
49A Chiba. Ontogeny of Serotonin-Immunoreactive Cells in the Gut Epithelium of theCloudy Dogfish, Scyliorhinus torazame, with Reference to Coexistence of Serotoninand Neuropeptide Y[J].General and Comparative Endocrinology,1998,111(3):290~298.
50G Tagliafierro, G G Rossi, E Bonini, et al. Ontogeny and Differentiation of Regulat-ory Peptide-and Serotonin-immunoreactivity in the Gastrointestinal Tract of anElasmobranch[J]. J Exp Zool Suppl,1989,252(S2):165~174.
51阚延胜.罗非鱼消化道5-HT内分泌细胞的免疫组织化学研究[J].安徽农学通报.2007,13(17):27-28.
52韩师昭,叶海辉,黄辉洋,等.大弹涂鱼消化道内分泌细胞的免疫组织化学研究[J].厦门大学学报(自然科学版).2005,44(3):407~409.
53王琼,叶海辉,黄辉洋,等.褐篮子鱼消化道内分泌细胞的鉴别与定位[J].海洋科学.2007,31(12):13~15.
54潘黔生,方之平.几种激素在鳜鱼胃肠道内分泌中存在的免疫细胞化学证据[J].水生生物学报.2001,25(6):565~570.
55舒妙安,徐海圣,朱有法,等.黄鳝胃肠的生长抑素分泌细胞及超微结构[J].水产学报.2002,26(3):206~212.
56方静,何敏.重口裂腹鱼消化道内分泌细胞的免疫组织化学鉴别和定位[J].水生生物学报.2007,31(5):744~747.
57帅细连,洪一江,林刚,等.萍乡肉红鲫肠道三种内分泌细胞的免疫组化定位分析[J].江西科学.2008,26(5):693~696.
58杨青,叶海辉,黄辉洋,等.黄鳍鲷消化道内分泌细胞的免疫组化定位[J].海洋通报.2005,24(4):87~90.
59叶海辉,朱艾嘉,黄辉洋,等.中华乌塘鳢消化道内分泌细胞的鉴别与定位[J].厦门大学学报(自然科学版).2006,45(4):545~548.
60A C Villaro, J Rovira, M E Bodegas, et al. Immunocytochemical and Ultrastructur-al Characterization of Endocrine Cells in the Larval Stomach of the Frog Ranatemporaria Tadpoles:a Comparison with Adult Specimens [J].Tissue and Cell,2001,33(5):462～477.
61李倩.中国林蛙(Rana chensinensis)消化管的胚后发育及5-HT和GAS细胞的发生[D].陕西师范大学.2008.
62李宁.中国大鲵胃肠道的胚后发育及其5-HT细胞的发生[D].陕西师范大学.2011.
63Hyeung-Sik Lee, Sae-Kwang Ku, and Jae-Hyun Lee. Changes in the Serotonin-,Somatostatin-and Motilin-immunoreactive Cells in the Gastrointestinal Tract of theBullfrog, Rana catesbeiana, at Various Developmental Stages[J]. Korean J Biol Sei,1999,3(1):97～102.
64C Maake, W Kloas, M Szendefi, et al. Neurohormonal Peptides, Serotonin, andNitric Oxide Synthase in the Enteric Nervous System and Endocrine Cells of theGastrointes-tinal Tract of Neotenic and Thyroid Hormone-treated Axolotls (Ambys-toma mexicanum)[J].Cell Tissue Res,1999,297(1):91~101.
65刘新波,曹雷,李淑兰.东方蝾螈消化道5-羟色胺免疫活性细胞的分布与形态学观察[J].四川动物.2011,30(3):409~411.
66欧阳凤,张文学,陈晓虹.叶氏隆肛蛙和太行隆肛蛙消化道5-羟色胺内分泌细胞[J].解剖学杂志.2007,30(4):493~495.
67SK Ku, HS Lee, JH Lee. Immunohistochemistry of Endocrine Cells in the Alim-entary Tract of the Tree Frog, Harborea japonica[J].Korean Biol Sci,2000,4(1):95~100.
68张秋金,陈友铃,耿宝荣,等.黑眶蟾蜍和黑斑蛙消化道5-羟色胺免疫活性细胞的免疫组织化学[J].动物学杂志.2005,40(4):17~21.
69甄靓靓,邱枫,李淑兰,等.非洲爪蟾消化道的5-羟色胺免疫活性细胞[J].解剖学杂志.2007,30(3):279~281.
70张盛周,张帅,刘婷婷,等.中华蟾蜍消化道内分泌细胞的分布与形态[J].解剖学杂志.2008,31(4):519~521.
71张盛周,薛立辉,陶少武.牛蛙胃肠胰系统内分泌细胞的免疫组织化学鉴定与定位[J].激光生物学报.2008,17(5):689~694.
72黄徐根,吴海龙,吴孝兵.日本林蛙胃肠道内分泌细胞的免疫组织化学[J].动物学杂志.2004,39(2):19~25.
73PC Arena, KC Richardson, J Yamada. An Immunohistochemical Study of EndocrineCells of the Alimentary Tract of the King's skink (Egernia kingii)[J]. J Anat.1990,170:73~85.
74甘静,李小叶,王淼,等.虎斑颈槽蛇消化道5-羟色胺免疫活性内分泌细胞的分布与形态学观察[J].四川动物.2009,28(2):244~246
75余中宾,张晓盼,王智超,等.无蹼壁虎消化道5-羟色胺细胞的免疫组织化学定位[J].中国组织化学与细胞化学杂志.2007,16(2):200~204.
76房振华,张秋金,耿宝荣.原尾蜥虎消化道5-羟色胺免疫反应细胞的免疫组织化学[J].福建师范大学学报(自然科学版).2009,25(5):86~89.
77刘超,甄靓靓,李淑兰,等.中华花龟消化道5-羟色胺免疫活性内分泌细胞的分布与形态学观察[J].四川动物.2007,26(2):274~277.
78张志强,吴孝兵.王锦蛇消化道5-羟色胺细胞的免疫组织化学定位[J].生物学杂志.2008,25(4):23~25.
79陈小青.短尾蝮蛇消化道生长抑素细胞的免疫组织化学观察[J].浙江大学学报（农业与生命科学版）.2002,28(3):307~308.
80MA Masini. Immunohistochemical Localization of Gut Peptides in the SmallIntestinal of Snakes. Bas.Appl.Histochem.1986,30:317~324
81李淑兰,高欣,王淼,等.丽斑麻蜥消化道内分泌细胞的免疫组织化学研究[J].解剖学报.2008,39(5):751~755.
82李淑兰,于永忠,高欣,等.白条草蜥消化道内分泌细胞的免疫组织化学[J].动物学杂志.2008,43(2):43~50.
83黄徐根,吴孝兵.蓝尾石龙子消化道内分泌细胞的免疫组织化学研究[J].解剖学报.2006,37(5):557~562.
84张志强,吴孝兵.红耳龟消化道内分泌细胞的免疫组织化学研究[J].淡水渔业.2009,39(3):68~72.
85MJ Saffrey, JM Polak, G Burnstock. Distribution of Vasoactive Intestinal Polypepti-de-,Substance P-, Enkephalin-and Neurotensin-like Immunoreactive Nerves in theChicken Gut during Development [J].Neuroscience,1982,7(l):279~293.
86曹雷,李淑兰.家鸽消化道5-羟色胺细胞的形态与分布[J].中国农学通报.2009,25(21):69~72.
87崔淼,李淑兰.锡嘴雀消化道5-HT细胞的免疫组织化学研究[J].中国农学通报.2010,26(21):63~66.
88沈元新,林支付,朱有法.绍兴家鸭幽门区生长抑素细胞的免疫组织化学研究[J].浙江大学学报.1999,25(6):623~626.
89J.N Yamada, T Kitamura, Yamashita, et al. Immonohistochemical Studies on theEndocrine Cells in Gvian Gizzard[J].Biomed Res.1986,7:39~45.
90Lucianna Maruccio, Carla Lucini, Finizia Russo, et al.The Development of AvianEnteric Nervous System:Distribution of Artemin Irnmuoreactivity[J]. Acta Histoeh-emica,2008,110:163~171.
91邓泽沛,周占祥,山田纯三.北京鸭消化道内分泌细胞的免疫组织化学研究[J].动物学报.1994,40(4):383~389.
92李丽霞,安书成.红腹锦鸡胃肠道内分泌细胞的免疫组织化学定位[J].动物学杂志.2009,44(1):24~32.
93葛仕豪,黄磊.禽类消化道内分泌细胞的免疫组化研究现状[J].家畜生态学报.2010,31(6):97~99.
94刘斌,曾少举,林永达,等.白鹮胃肠道内分泌细胞的免疫组织化学定位[J].动动物学研究.2004,25(5):442~446.
95TA Branchek, DG Michael. Time Course of Expression of Neuropeptide Y, Calciteo-nin Gene-related Peptide, and NADPH Diaphorase Activity in Neurons of theDeveloping Murine Bowel and the Appearance of5-hydroxytryptamine in MucosalEnterochromaffin Cells[J].J Comp Neurol,1989,285(2):262~273.
96赖为民,彭西,汪开毓.十二指肠、回肠、盲肠嗜银细胞生后发育的组织学变化[J].解剖学杂志.2010,33(5):610~612.
97LI Larsson, DM Hougaard. Sensitive Detection of Rat Gastrin mRNA by in Situhybridization with Chemically Biotinylated Oligodeoxynucleotides Validation,Quanti-tation, and Double-staining Studies[J].J Histoch Cytoch,1993,41(2):157~163.
98WJ Krause, J Yamada, JH Cutts. Enteroendocrine Cells in the Developing OpossumSmall Intestine and Colon[J].J Anat,1989,162:83~96.
99H Ito, J Yamada, N Kitamura, et al. Histological and Immunohistochemical Studiesof the Segi’s cap, a Large Aggregation of Endocrine Cells on the Intestinal Villi ofPorcine Fetuses and Neonates[J].Arch Histol Jpn,1985,48(4):399~409.
100王琳,梁文妹.胎儿小肠生长抑素、胃泌素及血管活性肠肽免疫反应细胞的个体发生[J].解剖学杂志.2005,28(4):436~439.
101李淑兰,陈洪岩.家兔消化道5-HT免疫活性细胞的免疫组织化学研究[J].中国比较医学杂志.2003,13(6):346~349.
102李丽霞,胡佳.棕色田鼠胃肠道5-羟色胺细胞的免疫组织化学定位[J].四川动物.2008,27(4):639~641.
103张盛周,王洋,孙晟.中国穿山甲消化道5羟色胺免疫活性细胞的定位[J].中国组织化学与细胞化学杂志.2008,17(2):200~203.
104杨倩,夏东,赵茹茜,等.猪胃生长抑素阳性细胞和盐酸细胞发育的研究.畜牧兽医学报.2003,34(5):461~464.
105张大红,王琳,韩正祥.豚鼠、大鼠和小鼠胃窦黏膜中胃泌素免疫反应细胞的形态学观察[J].徐州医学院学报.2004,24(3):239~241.
106刘湘江,杨隽,张磊,等.蓝狐消化道内分泌细胞的免疫组化研究[J].兽类学报.2009,29(3):338~342.
107陈磊,张洪海,马建章.紫貂消化道内分泌细胞形态和分布特征[J].北京林业大学学报.2010,32(2):127~132.
108陈磊,张洪海.狼和家犬消化道内分泌细胞免疫组织化学研究[J].解剖学报.2008,39(3):413~419.
109N Stanley, Salthe, S John, et a1. Eggs and Development[M]. Biology of Amphibia-ns,1974:127~131.
110M El-Salhy, E Winder, M Lundqvist. Comparative Studies of Serotonin-likeImmunoreactive Cells in the Digestive Tract of Vertebrates[J].Biomed Res,1985,6:371~375.
111张磊,刘湘江,董巍,等.不同生长期籽鹅消化道5-羟色胺的表达[J].解剖学杂志.2009,32(5):626~628.
112E Solcia, G Rindi, R Buffa, et a1. Gastric Endocrine Cells: Types, Function andGrowth[J]. Regul Pept,2000,93(1-3):31~35.
113赵文艳,李淑兰.黑斑蛙胃肠道嗜银细胞的分布及形态学研究[J].中国比较医学杂志.2003,13(6):364~367.
114周长文,楚德昌,李春华.巴西彩龟与无蹼壁虎消化道银染观察[J].四川动物.2008,27(2):230~235.
115王昱,俞诗源,贾艳芳,等.金钱豹胃和小肠的组织学及嗜银细胞的分布[J].解剖学杂志.2010,33(4):459~463.
116李淑兰,赵文阁,冷超,等.中国林蛙消化道4种内分泌细胞的免疫组织化学定位[J].解剖学杂志.2006,29(3):296~298.
117訾芙玮,李淑兰.家鸽消化道生长抑素免疫活性内分泌细胞的研究[J].中国农学通报.2010,26(14):23~26.
118侯衍猛,汤连升,王树迎,等.莱芜黑山羊消化道内分泌细胞的免疫组织化学研究[J].解剖学报.2007,38(5):585~588.
119张盛周,黄徐根,吴孝兵。棘胸蛙消化道内分泌细胞的免疫组织化学定位[J]。动物学报.2003,49(6):858~864.
120M El-Salhy, L Grimelius, E Wilander, et al. Histological and ImmunohistochemicalStudies of the Endocrine Cells of the Gastrointestinal Mucosa of the Toad (Buforegularis). Histochemistry,1981,71:53~65.
2刘美,李凤超,穆淑梅,等.半滑舌鳎胚胎发育组织学观察[J].四川动物.2008,27(5):737~739.
3Z Z Wan, T X Gao, X M Zhang, et al. Histological Study on the Digestive SystemDevelopment of Takifugu rubripes Larvae and Juvenile[J]. Journal of Ocean Univ-ersity of China,2006,1(5):39~44.
4林强,吕军仪,张彬.大海马消化系统胚后发育的形态学及组织学研究[J].带海洋学报.2007,26(6):46~51.
5关海红,匡友谊,徐伟,等.哲罗鱼消化系统器官发生发育的组织学观察[J].动物学杂志.2007,42(2):116~123.
6初庆柱,张健东,陈刚,等.军曹鱼消化系统胚后发育的组织学研究[J].水生态学杂志.2010,3(4):86~91.
7Hachero-Cruzado, JB Ortiz-Delgado, B Borrega, et al. Larval Organogenesis ofFlatfish Brill Scophthalmus rhombus L: Histological and Histochemical Aspects[J].Aquaeulture.2009,286(1-2):138~149.
8M P Garcia Hernandez, M T Lozano, M T Elbal, et al. Development of the Digesti-ve Tract of Sea Bass(Dicentrarchus labrax L).Light and Electron MicroscopicStudies[J]. Anat Embryol,2001,204(1):39~57.
9MI Sanchez-Amaya, JB Ortiz-Delgado, A Garcia-Lopez, et al. Larval Ontogeny ofRedbanded Seabream Pagrus aurigaValenciennes,1843with Special Reference tothe Digestive System. A Histological and Histochemical Approach[J].Aquaculture,2007,263(1-4):259~279.
10方华华,王波.星斑川鲽仔稚鱼消化系统发育的组织学研究[J].中国农学通报.2011,27(14):50~54.
11蒲德永,王志坚,赵海鹏,等.亚东鲑消化系统的形态学和组织学观察[J].四川动物.2006,25(4):825~828.
12J Rovira, A C Villaro, M E Bodegas, et al. Structural Study of the Frog Rana temp-oraria Larval Stomach[J].Tissue and Cell.1993,25(5):695~707.
13Atsuko Ishizuya-Oka. Epithelial Connective Tissue Cross Tale is Essential forRegene-ration of Intestinal Epithelium[J].J Nippon Med Sch.,2005.72(l):13~18.
14A Mary, Bonneville. Fine Structural Changes in the Intestinal Epithelium of theBull-frog during Metamorphosis[J].J Cell Biol,1963,18(3):579~597.
15M A Bowers. Histogenesis and Histolysis of the Intestinal Epithelium of Bufo lenti-ginosus [J]. Am J Anat,1909,9(1):263~279.
16王昱.花背蟾蜍消化道胚后发育的组织学观察[J].东北农业大学学报.2011,42(9):88~91.
17李宁,梁刚,刘婷婷.中国大鲵胃肠道胚后发育的解剖学与组织学观察[J].动物学杂志.2011,46(3):117~122.
18李倩,张育辉.中国林蛙消化管胚后发育的组织学观察[J].四川动物.2008,27(3):417~420.
19唐晓玲,刘振湘.不同发育期的牛蛙蝌蚪消化道组织学观察[J].湖北农学院学报.2001,21(4):332~334.
20王健鑫,金宇斌,王日昕.南移养殖中国林蛙早期胚胎发育的组织学研究[J].浙江农业学报.2008,20(4):245~249.
21华田苗,陈壁辉,王朝林.扬子鳄胚胎胃的组织发生[J].解剖学报.1996,27(2):221~224.
22李堃.扬子鳄胚胎消化系统内分泌细胞发生的免疫组化研究[D].芜湖:安徽师范大学.2002.
23赵宏霞,黄斌,赵万鹏.巴西彩龟消化道组织学的初步观察[J].四川动物.2010,29(3):437~439.
24华田苗,刘再群,陈士超,等.乌梢蛇胃上皮的组织学研究[J].动物学杂志.2001,36(6):7~10.
25甘文强,张晓雁,乔健,等.绿海龟消化道组织学观察[J].中国兽医杂志.2011,47(11):23~25.
26傅丽容,洪美玲,史海涛,等.中华花龟消化系统的组织学初步研究[J].四川动物.2007,26(2):270~273.
27李仲杰,杨友金,李继承.赤链华游蛇消化道粘膜上皮结构的扫描电镜观察[J].浙江大学学报(理学版).2003,30(4):453~456.
28刘忠虎,白晓辉,康相涛,等.父母代固始鸡十二指肠的发育形态学观察[J].中国兽医学报.2008,28(10):1181~1184.
29王政富,王丙云,卢玉葵,等.马冈鹅肠胚后发育的组织学观察[J].中国家禽学报.2003,7(1):28~31.
30黎观红,瞿明仁,晏向华,等.泰和鸡早期(0~12周龄)内脏器官生长发育规律的研究[J].经济动物学报.2005,9(1):35~38.
31王水亭.雏鸡消化道解剖与组织学特点[J].云南畜牧兽医.1999,1:10.
32刘玉堂,田秀华,于学伟,等.大鸨消化系统组织学观察[J].动物学杂志.2002,37(5):37~41.
33王丽萍，刘玉堂，肖向红，等.环颈雉消化系统组织形态学观察[J].动物学杂志.1994,29(3):26~28.
34张子慧,肖方,袁伟静,等.一雄性丹顶鹤消化系统组织形态学观察[J].动物学杂志.1999,34(3):39~41.
35王家乡,彭克美,杜安娜,等.非洲雏鸵鸟消化管的组织学观察[J].动物学杂志.2007,42(3):131~135.
36李铭,柳劲松.4种雀形目鸟消化道形态特征[J].动物学杂志.2008,43(l):116~121.
37张登荣,王新俭,王世英,等.兔生后胃、肝发育的组织学研究[J].河南职技师院学报.1991,19(4):33~36.
38张登荣,王世英,杜恒忠,等.兔生后舌和食管发育的组织学研究[J].中国兽医学报.1994,14(3):280~283.
39李树明,张方武,彭然,等.25日龄内哺乳仔猪消化系统发育研究[J].四川畜牧兽医学院学报.2002,16(3):6~10.
40王芳,余燕,牛红星.马铁菊头蝠消化系统的组织学观察[J].四川动物.2010,29(1):109~110.
41江剑平,林雪莹.赤腹松鼠(Callosciurus erythralus)消化道的解剖学和组织学研究[J].福建师范大学学报:自然科学版.2007,23(6):105~108.
42王平,曹悼,陈茂生.大熊猫Ailuropoda melanoleuca消化道的组织学观察[J].动物学研究.1982,3:27~28.
43刘进辉,谭理琦,王水莲,等.东北虎胃与小肠组织形态结构的研究[J].经济动物学报.2004,8(l):22~25.
44王志均,梅愈华,朱文玉.胃肠激素[M].北京:科学出版社.1985.1~10.
45W M Bayliss, E H Starling. The Mechanism of Pancreatic Secretion[J].J Phisiol,1902,28(5):325~353.
46T Fujita, S Kobayashi. Structure and Function of Gut Endocrine Cells. Int Rev CytolSuppl.1977,6:187~233.
47施志仪,陈晓武,顾一峰.牙鲆消化道组织学观察及内分泌细胞分布[J].中国水产科学.2006,13(5):851~855.
48M P Garcia Hernandez, M T Lozano, B Agulleiro. Ontogeny of Some EndocrineCells of the Digestive Tract in Sea Bass (Dicentrarchus labrax): An Immunocytoch-emical Study[J].Cell&Tissue Research,1994,277(2):373~383.
49A Chiba. Ontogeny of Serotonin-Immunoreactive Cells in the Gut Epithelium of theCloudy Dogfish, Scyliorhinus torazame, with Reference to Coexistence of Serotoninand Neuropeptide Y[J].General and Comparative Endocrinology,1998,111(3):290~298.
50G Tagliafierro, G G Rossi, E Bonini, et al. Ontogeny and Differentiation of Regulat-ory Peptide-and Serotonin-immunoreactivity in the Gastrointestinal Tract of anElasmobranch[J]. J Exp Zool Suppl,1989,252(S2):165~174.
51阚延胜.罗非鱼消化道5-HT内分泌细胞的免疫组织化学研究[J].安徽农学通报.2007,13(17):27-28.
52韩师昭,叶海辉,黄辉洋,等.大弹涂鱼消化道内分泌细胞的免疫组织化学研究[J].厦门大学学报(自然科学版).2005,44(3):407~409.
53王琼,叶海辉,黄辉洋,等.褐篮子鱼消化道内分泌细胞的鉴别与定位[J].海洋科学.2007,31(12):13~15.
54潘黔生,方之平.几种激素在鳜鱼胃肠道内分泌中存在的免疫细胞化学证据[J].水生生物学报.2001,25(6):565~570.
55舒妙安,徐海圣,朱有法,等.黄鳝胃肠的生长抑素分泌细胞及超微结构[J].水产学报.2002,26(3):206~212.
56方静,何敏.重口裂腹鱼消化道内分泌细胞的免疫组织化学鉴别和定位[J].水生生物学报.2007,31(5):744~747.
57帅细连,洪一江,林刚,等.萍乡肉红鲫肠道三种内分泌细胞的免疫组化定位分析[J].江西科学.2008,26(5):693~696.
58杨青,叶海辉,黄辉洋,等.黄鳍鲷消化道内分泌细胞的免疫组化定位[J].海洋通报.2005,24(4):87~90.
59叶海辉,朱艾嘉,黄辉洋,等.中华乌塘鳢消化道内分泌细胞的鉴别与定位[J].厦门大学学报(自然科学版).2006,45(4):545~548.
60A C Villaro, J Rovira, M E Bodegas, et al. Immunocytochemical and Ultrastructur-al Characterization of Endocrine Cells in the Larval Stomach of the Frog Ranatemporaria Tadpoles:a Comparison with Adult Specimens [J].Tissue and Cell,2001,33(5):462～477.
61李倩.中国林蛙(Rana chensinensis)消化管的胚后发育及5-HT和GAS细胞的发生[D].陕西师范大学.2008.
62李宁.中国大鲵胃肠道的胚后发育及其5-HT细胞的发生[D].陕西师范大学.2011.
63Hyeung-Sik Lee, Sae-Kwang Ku, and Jae-Hyun Lee. Changes in the Serotonin-,Somatostatin-and Motilin-immunoreactive Cells in the Gastrointestinal Tract of theBullfrog, Rana catesbeiana, at Various Developmental Stages[J]. Korean J Biol Sei,1999,3(1):97～102.
64C Maake, W Kloas, M Szendefi, et al. Neurohormonal Peptides, Serotonin, andNitric Oxide Synthase in the Enteric Nervous System and Endocrine Cells of theGastrointes-tinal Tract of Neotenic and Thyroid Hormone-treated Axolotls (Ambys-toma mexicanum)[J].Cell Tissue Res,1999,297(1):91~101.
65刘新波,曹雷,李淑兰.东方蝾螈消化道5-羟色胺免疫活性细胞的分布与形态学观察[J].四川动物.2011,30(3):409~411.
66欧阳凤,张文学,陈晓虹.叶氏隆肛蛙和太行隆肛蛙消化道5-羟色胺内分泌细胞[J].解剖学杂志.2007,30(4):493~495.
67SK Ku, HS Lee, JH Lee. Immunohistochemistry of Endocrine Cells in the Alim-entary Tract of the Tree Frog, Harborea japonica[J].Korean Biol Sci,2000,4(1):95~100.
68张秋金,陈友铃,耿宝荣,等.黑眶蟾蜍和黑斑蛙消化道5-羟色胺免疫活性细胞的免疫组织化学[J].动物学杂志.2005,40(4):17~21.
69甄靓靓,邱枫,李淑兰,等.非洲爪蟾消化道的5-羟色胺免疫活性细胞[J].解剖学杂志.2007,30(3):279~281.
70张盛周,张帅,刘婷婷,等.中华蟾蜍消化道内分泌细胞的分布与形态[J].解剖学杂志.2008,31(4):519~521.
71张盛周,薛立辉,陶少武.牛蛙胃肠胰系统内分泌细胞的免疫组织化学鉴定与定位[J].激光生物学报.2008,17(5):689~694.
72黄徐根,吴海龙,吴孝兵.日本林蛙胃肠道内分泌细胞的免疫组织化学[J].动物学杂志.2004,39(2):19~25.
73PC Arena, KC Richardson, J Yamada. An Immunohistochemical Study of EndocrineCells of the Alimentary Tract of the King's skink (Egernia kingii)[J]. J Anat.1990,170:73~85.
74甘静,李小叶,王淼,等.虎斑颈槽蛇消化道5-羟色胺免疫活性内分泌细胞的分布与形态学观察[J].四川动物.2009,28(2):244~246
75余中宾,张晓盼,王智超,等.无蹼壁虎消化道5-羟色胺细胞的免疫组织化学定位[J].中国组织化学与细胞化学杂志.2007,16(2):200~204.
76房振华,张秋金,耿宝荣.原尾蜥虎消化道5-羟色胺免疫反应细胞的免疫组织化学[J].福建师范大学学报(自然科学版).2009,25(5):86~89.
77刘超,甄靓靓,李淑兰,等.中华花龟消化道5-羟色胺免疫活性内分泌细胞的分布与形态学观察[J].四川动物.2007,26(2):274~277.
78张志强,吴孝兵.王锦蛇消化道5-羟色胺细胞的免疫组织化学定位[J].生物学杂志.2008,25(4):23~25.
79陈小青.短尾蝮蛇消化道生长抑素细胞的免疫组织化学观察[J].浙江大学学报（农业与生命科学版）.2002,28(3):307~308.
80MA Masini. Immunohistochemical Localization of Gut Peptides in the SmallIntestinal of Snakes. Bas.Appl.Histochem.1986,30:317~324
81李淑兰,高欣,王淼,等.丽斑麻蜥消化道内分泌细胞的免疫组织化学研究[J].解剖学报.2008,39(5):751~755.
82李淑兰,于永忠,高欣,等.白条草蜥消化道内分泌细胞的免疫组织化学[J].动物学杂志.2008,43(2):43~50.
83黄徐根,吴孝兵.蓝尾石龙子消化道内分泌细胞的免疫组织化学研究[J].解剖学报.2006,37(5):557~562.
84张志强,吴孝兵.红耳龟消化道内分泌细胞的免疫组织化学研究[J].淡水渔业.2009,39(3):68~72.
85MJ Saffrey, JM Polak, G Burnstock. Distribution of Vasoactive Intestinal Polypepti-de-,Substance P-, Enkephalin-and Neurotensin-like Immunoreactive Nerves in theChicken Gut during Development [J].Neuroscience,1982,7(l):279~293.
86曹雷,李淑兰.家鸽消化道5-羟色胺细胞的形态与分布[J].中国农学通报.2009,25(21):69~72.
87崔淼,李淑兰.锡嘴雀消化道5-HT细胞的免疫组织化学研究[J].中国农学通报.2010,26(21):63~66.
88沈元新,林支付,朱有法.绍兴家鸭幽门区生长抑素细胞的免疫组织化学研究[J].浙江大学学报.1999,25(6):623~626.
89J.N Yamada, T Kitamura, Yamashita, et al. Immonohistochemical Studies on theEndocrine Cells in Gvian Gizzard[J].Biomed Res.1986,7:39~45.
90Lucianna Maruccio, Carla Lucini, Finizia Russo, et al.The Development of AvianEnteric Nervous System:Distribution of Artemin Irnmuoreactivity[J]. Acta Histoeh-emica,2008,110:163~171.
91邓泽沛,周占祥,山田纯三.北京鸭消化道内分泌细胞的免疫组织化学研究[J].动物学报.1994,40(4):383~389.
92李丽霞,安书成.红腹锦鸡胃肠道内分泌细胞的免疫组织化学定位[J].动物学杂志.2009,44(1):24~32.
93葛仕豪,黄磊.禽类消化道内分泌细胞的免疫组化研究现状[J].家畜生态学报.2010,31(6):97~99.
94刘斌,曾少举,林永达,等.白鹮胃肠道内分泌细胞的免疫组织化学定位[J].动动物学研究.2004,25(5):442~446.
95TA Branchek, DG Michael. Time Course of Expression of Neuropeptide Y, Calciteo-nin Gene-related Peptide, and NADPH Diaphorase Activity in Neurons of theDeveloping Murine Bowel and the Appearance of5-hydroxytryptamine in MucosalEnterochromaffin Cells[J].J Comp Neurol,1989,285(2):262~273.
96赖为民,彭西,汪开毓.十二指肠、回肠、盲肠嗜银细胞生后发育的组织学变化[J].解剖学杂志.2010,33(5):610~612.
97LI Larsson, DM Hougaard. Sensitive Detection of Rat Gastrin mRNA by in Situhybridization with Chemically Biotinylated Oligodeoxynucleotides Validation,Quanti-tation, and Double-staining Studies[J].J Histoch Cytoch,1993,41(2):157~163.
98WJ Krause, J Yamada, JH Cutts. Enteroendocrine Cells in the Developing OpossumSmall Intestine and Colon[J].J Anat,1989,162:83~96.
99H Ito, J Yamada, N Kitamura, et al. Histological and Immunohistochemical Studiesof the Segi’s cap, a Large Aggregation of Endocrine Cells on the Intestinal Villi ofPorcine Fetuses and Neonates[J].Arch Histol Jpn,1985,48(4):399~409.
100王琳,梁文妹.胎儿小肠生长抑素、胃泌素及血管活性肠肽免疫反应细胞的个体发生[J].解剖学杂志.2005,28(4):436~439.
101李淑兰,陈洪岩.家兔消化道5-HT免疫活性细胞的免疫组织化学研究[J].中国比较医学杂志.2003,13(6):346~349.
102李丽霞,胡佳.棕色田鼠胃肠道5-羟色胺细胞的免疫组织化学定位[J].四川动物.2008,27(4):639~641.
103张盛周,王洋,孙晟.中国穿山甲消化道5羟色胺免疫活性细胞的定位[J].中国组织化学与细胞化学杂志.2008,17(2):200~203.
104杨倩,夏东,赵茹茜,等.猪胃生长抑素阳性细胞和盐酸细胞发育的研究.畜牧兽医学报.2003,34(5):461~464.
105张大红,王琳,韩正祥.豚鼠、大鼠和小鼠胃窦黏膜中胃泌素免疫反应细胞的形态学观察[J].徐州医学院学报.2004,24(3):239~241.
106刘湘江,杨隽,张磊,等.蓝狐消化道内分泌细胞的免疫组化研究[J].兽类学报.2009,29(3):338~342.
107陈磊,张洪海,马建章.紫貂消化道内分泌细胞形态和分布特征[J].北京林业大学学报.2010,32(2):127~132.
108陈磊,张洪海.狼和家犬消化道内分泌细胞免疫组织化学研究[J].解剖学报.2008,39(3):413~419.
109N Stanley, Salthe, S John, et a1. Eggs and Development[M]. Biology of Amphibia-ns,1974:127~131.
110M El-Salhy, E Winder, M Lundqvist. Comparative Studies of Serotonin-likeImmunoreactive Cells in the Digestive Tract of Vertebrates[J].Biomed Res,1985,6:371~375.
111张磊,刘湘江,董巍,等.不同生长期籽鹅消化道5-羟色胺的表达[J].解剖学杂志.2009,32(5):626~628.
112E Solcia, G Rindi, R Buffa, et a1. Gastric Endocrine Cells: Types, Function andGrowth[J]. Regul Pept,2000,93(1-3):31~35.
113赵文艳,李淑兰.黑斑蛙胃肠道嗜银细胞的分布及形态学研究[J].中国比较医学杂志.2003,13(6):364~367.
114周长文,楚德昌,李春华.巴西彩龟与无蹼壁虎消化道银染观察[J].四川动物.2008,27(2):230~235.
115王昱,俞诗源,贾艳芳,等.金钱豹胃和小肠的组织学及嗜银细胞的分布[J].解剖学杂志.2010,33(4):459~463.
116李淑兰,赵文阁,冷超,等.中国林蛙消化道4种内分泌细胞的免疫组织化学定位[J].解剖学杂志.2006,29(3):296~298.
117訾芙玮,李淑兰.家鸽消化道生长抑素免疫活性内分泌细胞的研究[J].中国农学通报.2010,26(14):23~26.
118侯衍猛,汤连升,王树迎,等.莱芜黑山羊消化道内分泌细胞的免疫组织化学研究[J].解剖学报.2007,38(5):585~588.
119张盛周,黄徐根,吴孝兵。棘胸蛙消化道内分泌细胞的免疫组织化学定位[J]。动物学报.2003,49(6):858~864.
120M El-Salhy, L Grimelius, E Wilander, et al. Histological and ImmunohistochemicalStudies of the Endocrine Cells of the Gastrointestinal Mucosa of the Toad (Buforegularis). Histochemistry,1981,71:53~65.