细胞周期蛋白-D1和Ki-67在喉癌组织中的表达及相关性研究
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摘要
喉癌是头颈部最常见的恶性肿瘤,在我国该病约占全身肿瘤的1%～5%,其病理类型以鳞状细胞癌为主,占全部喉癌的93%～99%。我国北方地区喉癌的发病率较高,发病年龄50～60岁为最多,男性的发病率高于女性。近年来,随着工业污染的加重,喉癌的发病率有明显的上升趋势。喉癌治疗涉及手术、放射治疗、抗肿瘤药物治疗和基因治疗等综合措施。随着诊断和治疗水平的不断进步,喉癌患者手术后喉功能保留率、生活质量及5年生存率均有了较大的提高,但是晚期喉癌患者的治疗效果仍不尽如人意,局部复发和转移仍为喉癌患者术后5年内死亡的主要原因。作为喉癌早期治疗的微创手术,也要求对喉癌早期诊断和治疗预后有正确的估计,以尽量减少创伤,保留患者的喉功能。因此,作为临床应用基础研究的目的之一,探讨与喉癌发生、发展的相关因素,以期寻找一个有效的检测指标以协助诊断、尽早的区分其病理类型,了解病变的范围以预测肿瘤的预后,有助于确定合理的治疗方案和治疗预期。
喉腔黏膜的上皮细胞发生癌变的具体机制不明,可能与吸烟、饮酒、环境污染、病毒感染等因素有关。随着对细胞调控认识的不断深入,研究发现：细胞周期的调控异常是癌症发病的一个重要环节,细胞周期蛋白(Cyclins)作为细胞周期的正向调控因素,控制细胞周期的进程,其表达水平具有细胞周期依赖性,是调节细胞活动的重要蛋白质。Cyclins的异常表达是导致细胞周期调控机制受到破坏的主要因素之一,与恶性肿瘤的发生密切相关。Cyclins在肿瘤的发生发展中所扮演的角色日益成为人们关注的焦点,认为：癌症等恶性肿瘤可能是一类细胞周期性疾病。
Ki-67是增殖性细胞核的标记物,代表细胞增殖水平,目前认为：Ki-67的功能与细胞有丝分裂有关。分子生物学研究表明：细胞过度分裂与增殖是肿瘤恶变的重要环节。Ki-67在G1后期出现,S、G2期升高,M期达峰值,只参与增生细胞核反应,于GO期消失,进入G1期时开始表达至有丝分裂后迅速减少,到GO期则不表达。许多研究表明：Ki-67蛋白表达的精确调控、分布,与很多恶性肿瘤的发展、转移、预后,均具相关性,是细胞增殖、完成细胞周期所不可或缺的,是检测肿瘤细胞增殖活性的重要指标,可作为肿瘤预后的经典标记物。Ki-67蛋白临床检测已被广泛开展,但是在喉肿瘤预后及治疗方面的研究应用在近几年才被逐渐报道。
本研究通过检测Cyclin-D1和Ki-67在喉鳞状细胞癌组织、癌旁组织、喉腔正常鳞状上皮组织中的表达情况,以期为喉癌的早期诊断和预后判断提供一个可靠的分子生物学指标。
目的
初步探讨细胞周期蛋白D1和细胞增殖抗原标记物Ki-67在喉鳞状细胞癌组织中的表达及其与临床、病理特征之间的关系,以及两者在喉鳞状细胞癌组织中表达的相关性,以期为喉癌的早期诊断和预后判断提供一个可靠的分子生物学指标。
方法
92例我院2000年1月至2005年8月期间收治的喉鳞状细胞癌患者行根治性手术后所留取的标本,其中男性78例,女性14例。年龄36～79岁,平均55.4岁。根据肿瘤原发部位分为：声门上型43例,声门型46例,声门下型3例；根据肿瘤分化程度：高分化(G1)25例；中分化(G2)19例；低分化(G3)48例；根据癌肿的生长范围和扩展的程度,按国际抗癌协会(UICC)2002年第6版公布的TNM分类分期方案如下：Ⅰ期6例；Ⅱ期15例；Ⅲ期46例；Ⅳ期25例。应用免疫组化S-P法检测Cyclin-D1和Ki-67的蛋白表达,以PBS代替一抗作为阴性对照,比较上述基因蛋白在正常喉部鳞状组织(对照1组)、喉鳞状细胞癌组织、喉癌旁组织(对照2组)中的表达情况,探讨Cyclin-D1和Ki-67在喉癌组织中的表达与患者临床分期、病理特征之间的关系以及两者之间的相关性。
采用SPSS12.0统计软件进行统计。喉鳞状细胞癌组织、喉腔正常鳞状上皮组织(对照1组)、肿瘤旁组织(对照2组)之间比较,计量资料以(x±s)表示,以one-way ANOVA方法进行总体比较,t检验做组间比较；Cyclin-D1和Ki-67在喉癌组织中的表达与患者临床病理特征之间的关系比较采用四格表资料的χ2检验,Cyclin-D1和Ki-67在喉癌组织中的表达的相关性比较采用Spearman相关分析,检验显著性水准取a=0.05,以P<0.05为差异有统计学意义。
结果
(1)在92个肿瘤样本中,有60例(65.2%)检测到有细胞周期蛋白D1的表达,而在对照1组和2组中,检出率分别为3%(3/100)和13.1%(12/92),三组对于细胞周期蛋白D1的检出率具有显著性差异(P<0.05),而且进一步分析发现：在不同分期的喉鳞状细胞癌组织中,Cyclin-D1的表达与临床分期之间有统计学相关性(P=0.029<0.05)。然而,Cyclin-D1的表达和其他变量(性别、年龄、癌症的阶段、组织分化、解剖学部位、吸烟史、饮酒史)之间没有统计学相关性。
(2)Ki-67免疫组化检测：Ki-67阳性信号主要定位于细胞质,为粗细不等的棕黄或棕褐色颗粒。正常喉部鳞状细胞上皮组织中未见表达,喉癌旁组织中有4例(4.34%)呈弱阳性表达。研究发现：Ki-67的过度表达与肿瘤患者的性别、年龄、解剖学部位、组织分化、吸烟史和饮酒史无统计学相关性。但Ki-67蛋白表达与患者颈部淋巴结转移状态有显著相关关系(P=0.025<0.05)。
(3)Ki-67蛋白的表达与患者的生存率无相关性,Ki-67蛋白的表达与生存时间无统计学相关关系(P=0.457)。而Cyclin-D1蛋白在喉鳞状细胞癌组织中的表达与患者的生存率有明显的相关关系(P=0.0004<0.05)。
(4)在Ki-67阳性高表达的56例喉癌组织中,有41例出现Cyclin D1阳性高表达；在Cyclin Dl阳性低度表达的51例患者中,有36例出现Ki-67阳性低度表达,经Spearman等级相关性检验表明：两者在喉鳞状细胞癌组织中的表达呈显著正相关关系(r=0.620,P<0.001)。
结论
(1)Cyclin D1在喉鳞状细胞癌组织中表达率高,与患者的临床分期及预后有显著的相关关系,Cyclin D1低表达者有较高的生存率,可作为喉鳞状细胞癌术后的监测指标,为喉鳞状细胞癌的治疗提供一个新的靶点。
(2)Ki-67在喉鳞状细胞癌组织中亦有表达,与患者颈部淋巴结转移状态有关,Ki-67高表达与淋巴结转移率高有显著的相关关系,可作为喉鳞状细胞癌患者淋巴结转移的监测指标。
(3)Cyclin D1和Ki-67在喉鳞状细胞癌组织中的表达具有显著正相关关系,两者联合检测对喉鳞状细胞癌患者术后辅助治疗及预后判断有一定的指导意义。
Laryngeal cancer is the most common malignant tumors of the head and neck, accounting for about1%to5%of the systemic tumors in our body, and the pathological type of squamous cell carcinoma, accounts for93%to99%of all laryngeal cancer. Higher incidence of laryngeal cancer is in northern China, and the age of onset is50to60years old and male with a high incidence than women. In recent years, with industrial pollution increased, the incidence of laryngeal cancer has a clear upward trend. Comprehensive treatment of laryngeal cancer include surgery, application of anticancer drug therapy, radiation therapy and gene therapy. With the continuous advancement of diagnosis and treatment,patients with laryngeal cancer after repair function, quality of life and5-year survival rates are greatly improved, but local recurrence and metastasis is still the main reason for laryngeal cancer patients died within5years and the treatment of patients with advanced laryngeal cancer is still not satisfactory. On the other hand, minimally invasive surgery also requires a correct estimate of laryngeal cancer early diagnosis and prognosis of laryngeal cancer, in order to minimize the trauma to retain the function of the patient's throat. Explore the relevant factors related to the incidence of laryngeal cancer, in order to find an effective indicator of the detection of early distinction between the pathological type, to understand the extent of the tumor staging, to determine a reasonable treatment options, prognosis, has become one of the key clinical research.
Laryngeal epithelial cells to become cancerous mechanism is unknown, may be related to smoking, alcohol consumption, environmental pollution, the virus infection and other factors. Deeper understanding of cellular regulation, abnormal regulation of cell cycle was found to be an important part of cancer incidence, and cell cycle proteins (cyclins) as a positive cell cycle regulatory factors that control cell cycle progression, the expression levels was cell cycle dependent, and it was an important protein for regulating cell activities. The abnormal expression of cyclins is one of the main factors leading to damage of cell cycle control mechanisms, is closely related to the occurrence of malignant tumors. That cyclins play a role in tumor development is increasingly becoming the focus of attention and the emergence of cancer may be a class of cells in view of cyclical disease.
Ki-67is a proliferating cell nuclear marker, representing the level of cell proliferation.The function of Ki-67is related to cell mitosis. Molecular biology studies have shown that cell over-division and proliferation is an important part of tumor malignant. The Ki-67appears in late G1, S-G2period is elevated,M phase reaches its peak, only with proliferating cell nuclear reaction, disappears in the GO phase. Many studies have shown that precise regulation of Ki-67protein expression, distribution are relevant to the development,metastasis and prognosis of many malignant tumors.It is indispensable to cell proliferation, completing the cell cycle.And it is the detection of tumor cell proliferative activity indicator.It can be used as a classic prognostic marker. Ki-67proteins are now widely on the application, but in cancer research, especially in the prognosis and treatment of laryngeal tumors of research in recent years gradually been reported.
In this study, expression of cyclin-D1and Ki-67in laryngeal squamous cell carcinoma and adjacent tissues, throat normal squamous epithelial tissue of laryngeal cancer are detected in order to provide a reliable molecular markers for early diagnosis and prognosis of laryngeal cancer.
Objective
To study the cell cycle protein D1and cell proliferation antigen markers Ki-67in laryngeal cancer tissue of the expression and the clinical, pathological, the relationship between the two groups as well as the correlation of expression.
Methods
92cases of laryngeal cancer patients after radical surgery sacrificing the specimens from January2000to August2005, including78cases of male,14cases of female. Aged36to79years old, average55.4years old. The tumor:43cases of supraglottic,46cases of glottic and subglottic, three cases. According to the1997International Anti-Cancer Association (UICC) TNM type and phased program, the degree of tumor differentiation:well differentiated (Gl),25cases; moderately differentiated (G2),19cases; poorly differentiated (G3),48cases; TNM clinical stage,6cases of stage Ⅰ;15cases Ⅱ;46cases Ⅲ; Ⅳ of25cases. The immunohistochemical SP staining method is used to detect the expression of cyclin D1and Ki-67protein, with PBS instead of primary antibody as a negative control, and compare the expression of these gene protein in the normal larynx squamous (control group1), laryngeal squamous cell carcinoma,laryngeal tissues (control group2).To study the relationship between the expression of cyclin D1and Ki-67and clinical pathological features and its correlation. The statistical software SPSS12.0statistics. Throat cancer organizations, tumor tissue adjacent group and control group, and between the measurement data to (s) says that on one-way ANOVA method in overall comparison, t test between groups do more, Spearman rank correlation analysis and tests significant level take α=0.05, P<0.05for differences with a statistical significance.
Results
(1) in92tumor samples60patients (65.2%) were observed the cell cycle protein Dl expression, and in the control group1and the group2, and the detection rate were3%(3/100) and13.1%(12/92), three groups for the cell cycle protein D1detection rate with significant difference (P<0.05), and further testing is found in different stages, the laryngeal cancer cells, the organization cell cycle protein D1expression and the clinical stages of the relevance of statistically significant (P=0.029). However, was found in cell cycle protein expression and other variables D1(gender, age, cancer stage, organization, anatomy, part differentiation smoking history, drinking histories) no statistical correlation.
(2) Ki-67immunohistochemical detection results:five element positive signal main role in cytoplasm, as of different thicknesses of palm or brown particles. Normal squamous organization has not seen the throat expression, laryngeal cancer adjacent tissues with a small amount of expression,4cases (4.34%) of the thyroid gland tumor is weak positive expression. And according to the results, Ki-67excessive expression and tumors of patients of gender, age, high differentiation, anatomy site, organization differentiation, smoking history and drinking history is no statistical correlation (see table2). Ki-67expression and lymph node state has a significantly related (P=0.025).
(3) Ki-67expression may and patient surial rates have relevance, Ki-67expression and survival time was statistically relevant (P=0.457). In addition, the cell cycle protein D1surial for patients with expression and the statistical correlation (P=0.0004),
(4) in the Cyclin D1positive oer-expressed in57throat breast cancer organization,41cases Ki-67positive expression. In Ki-67positive low-grade expression of51patients had35patients Cyclin Dl positive low-grade express, the spearman rank related inspection both in the throat papilloma group expression is a significant positive correlation (r=0.620, P<0.001).
Conclusions
(1) Cyclin D1expression in laryngeal squamous cell carcinoma is high.There is a significant relationship with clinical stage and prognosis of the patients. Those with low expression of Cyclin D1have a higher survival rate. Cyclin D1can be used as a laryngeal squamous cell carcinoma postoperative monitoring indicators, and provide a new target for the treatment of laryngeal squamous cell carcinoma.
(2) Ki-67is also expressed in laryngeal squamous cell carcinoma tissues. There is a significant relationship between the expression and neck lymph node metastasis of the patients.Ki-67expression can be used as a lymph node metastasis monitoring indicators of the patients with laryngeal squamous cell carcinoma.
(3) Cyclin D1and Ki-67expression in laryngeal squamous cell carcinoma has a significantly positive correlation. They can be used as a monitoring indicators of lymphnode metastasis and prognosis of patients with laryngeal squamous cell carcinoma after operation.
喉腔黏膜的上皮细胞发生癌变的具体机制不明,可能与吸烟、饮酒、环境污染、病毒感染等因素有关。随着对细胞调控认识的不断深入,研究发现：细胞周期的调控异常是癌症发病的一个重要环节,细胞周期蛋白(Cyclins)作为细胞周期的正向调控因素,控制细胞周期的进程,其表达水平具有细胞周期依赖性,是调节细胞活动的重要蛋白质。Cyclins的异常表达是导致细胞周期调控机制受到破坏的主要因素之一,与恶性肿瘤的发生密切相关。Cyclins在肿瘤的发生发展中所扮演的角色日益成为人们关注的焦点,认为：癌症等恶性肿瘤可能是一类细胞周期性疾病。
Ki-67是增殖性细胞核的标记物,代表细胞增殖水平,目前认为：Ki-67的功能与细胞有丝分裂有关。分子生物学研究表明：细胞过度分裂与增殖是肿瘤恶变的重要环节。Ki-67在G1后期出现,S、G2期升高,M期达峰值,只参与增生细胞核反应,于GO期消失,进入G1期时开始表达至有丝分裂后迅速减少,到GO期则不表达。许多研究表明：Ki-67蛋白表达的精确调控、分布,与很多恶性肿瘤的发展、转移、预后,均具相关性,是细胞增殖、完成细胞周期所不可或缺的,是检测肿瘤细胞增殖活性的重要指标,可作为肿瘤预后的经典标记物。Ki-67蛋白临床检测已被广泛开展,但是在喉肿瘤预后及治疗方面的研究应用在近几年才被逐渐报道。
本研究通过检测Cyclin-D1和Ki-67在喉鳞状细胞癌组织、癌旁组织、喉腔正常鳞状上皮组织中的表达情况,以期为喉癌的早期诊断和预后判断提供一个可靠的分子生物学指标。
目的
初步探讨细胞周期蛋白D1和细胞增殖抗原标记物Ki-67在喉鳞状细胞癌组织中的表达及其与临床、病理特征之间的关系,以及两者在喉鳞状细胞癌组织中表达的相关性,以期为喉癌的早期诊断和预后判断提供一个可靠的分子生物学指标。
方法
92例我院2000年1月至2005年8月期间收治的喉鳞状细胞癌患者行根治性手术后所留取的标本,其中男性78例,女性14例。年龄36～79岁,平均55.4岁。根据肿瘤原发部位分为：声门上型43例,声门型46例,声门下型3例；根据肿瘤分化程度：高分化(G1)25例；中分化(G2)19例；低分化(G3)48例；根据癌肿的生长范围和扩展的程度,按国际抗癌协会(UICC)2002年第6版公布的TNM分类分期方案如下：Ⅰ期6例；Ⅱ期15例；Ⅲ期46例；Ⅳ期25例。应用免疫组化S-P法检测Cyclin-D1和Ki-67的蛋白表达,以PBS代替一抗作为阴性对照,比较上述基因蛋白在正常喉部鳞状组织(对照1组)、喉鳞状细胞癌组织、喉癌旁组织(对照2组)中的表达情况,探讨Cyclin-D1和Ki-67在喉癌组织中的表达与患者临床分期、病理特征之间的关系以及两者之间的相关性。
采用SPSS12.0统计软件进行统计。喉鳞状细胞癌组织、喉腔正常鳞状上皮组织(对照1组)、肿瘤旁组织(对照2组)之间比较,计量资料以(x±s)表示,以one-way ANOVA方法进行总体比较,t检验做组间比较；Cyclin-D1和Ki-67在喉癌组织中的表达与患者临床病理特征之间的关系比较采用四格表资料的χ2检验,Cyclin-D1和Ki-67在喉癌组织中的表达的相关性比较采用Spearman相关分析,检验显著性水准取a=0.05,以P<0.05为差异有统计学意义。
结果
(1)在92个肿瘤样本中,有60例(65.2%)检测到有细胞周期蛋白D1的表达,而在对照1组和2组中,检出率分别为3%(3/100)和13.1%(12/92),三组对于细胞周期蛋白D1的检出率具有显著性差异(P<0.05),而且进一步分析发现：在不同分期的喉鳞状细胞癌组织中,Cyclin-D1的表达与临床分期之间有统计学相关性(P=0.029<0.05)。然而,Cyclin-D1的表达和其他变量(性别、年龄、癌症的阶段、组织分化、解剖学部位、吸烟史、饮酒史)之间没有统计学相关性。
(2)Ki-67免疫组化检测：Ki-67阳性信号主要定位于细胞质,为粗细不等的棕黄或棕褐色颗粒。正常喉部鳞状细胞上皮组织中未见表达,喉癌旁组织中有4例(4.34%)呈弱阳性表达。研究发现：Ki-67的过度表达与肿瘤患者的性别、年龄、解剖学部位、组织分化、吸烟史和饮酒史无统计学相关性。但Ki-67蛋白表达与患者颈部淋巴结转移状态有显著相关关系(P=0.025<0.05)。
(3)Ki-67蛋白的表达与患者的生存率无相关性,Ki-67蛋白的表达与生存时间无统计学相关关系(P=0.457)。而Cyclin-D1蛋白在喉鳞状细胞癌组织中的表达与患者的生存率有明显的相关关系(P=0.0004<0.05)。
(4)在Ki-67阳性高表达的56例喉癌组织中,有41例出现Cyclin D1阳性高表达；在Cyclin Dl阳性低度表达的51例患者中,有36例出现Ki-67阳性低度表达,经Spearman等级相关性检验表明：两者在喉鳞状细胞癌组织中的表达呈显著正相关关系(r=0.620,P<0.001)。
结论
(1)Cyclin D1在喉鳞状细胞癌组织中表达率高,与患者的临床分期及预后有显著的相关关系,Cyclin D1低表达者有较高的生存率,可作为喉鳞状细胞癌术后的监测指标,为喉鳞状细胞癌的治疗提供一个新的靶点。
(2)Ki-67在喉鳞状细胞癌组织中亦有表达,与患者颈部淋巴结转移状态有关,Ki-67高表达与淋巴结转移率高有显著的相关关系,可作为喉鳞状细胞癌患者淋巴结转移的监测指标。
(3)Cyclin D1和Ki-67在喉鳞状细胞癌组织中的表达具有显著正相关关系,两者联合检测对喉鳞状细胞癌患者术后辅助治疗及预后判断有一定的指导意义。
Laryngeal cancer is the most common malignant tumors of the head and neck, accounting for about1%to5%of the systemic tumors in our body, and the pathological type of squamous cell carcinoma, accounts for93%to99%of all laryngeal cancer. Higher incidence of laryngeal cancer is in northern China, and the age of onset is50to60years old and male with a high incidence than women. In recent years, with industrial pollution increased, the incidence of laryngeal cancer has a clear upward trend. Comprehensive treatment of laryngeal cancer include surgery, application of anticancer drug therapy, radiation therapy and gene therapy. With the continuous advancement of diagnosis and treatment,patients with laryngeal cancer after repair function, quality of life and5-year survival rates are greatly improved, but local recurrence and metastasis is still the main reason for laryngeal cancer patients died within5years and the treatment of patients with advanced laryngeal cancer is still not satisfactory. On the other hand, minimally invasive surgery also requires a correct estimate of laryngeal cancer early diagnosis and prognosis of laryngeal cancer, in order to minimize the trauma to retain the function of the patient's throat. Explore the relevant factors related to the incidence of laryngeal cancer, in order to find an effective indicator of the detection of early distinction between the pathological type, to understand the extent of the tumor staging, to determine a reasonable treatment options, prognosis, has become one of the key clinical research.
Laryngeal epithelial cells to become cancerous mechanism is unknown, may be related to smoking, alcohol consumption, environmental pollution, the virus infection and other factors. Deeper understanding of cellular regulation, abnormal regulation of cell cycle was found to be an important part of cancer incidence, and cell cycle proteins (cyclins) as a positive cell cycle regulatory factors that control cell cycle progression, the expression levels was cell cycle dependent, and it was an important protein for regulating cell activities. The abnormal expression of cyclins is one of the main factors leading to damage of cell cycle control mechanisms, is closely related to the occurrence of malignant tumors. That cyclins play a role in tumor development is increasingly becoming the focus of attention and the emergence of cancer may be a class of cells in view of cyclical disease.
Ki-67is a proliferating cell nuclear marker, representing the level of cell proliferation.The function of Ki-67is related to cell mitosis. Molecular biology studies have shown that cell over-division and proliferation is an important part of tumor malignant. The Ki-67appears in late G1, S-G2period is elevated,M phase reaches its peak, only with proliferating cell nuclear reaction, disappears in the GO phase. Many studies have shown that precise regulation of Ki-67protein expression, distribution are relevant to the development,metastasis and prognosis of many malignant tumors.It is indispensable to cell proliferation, completing the cell cycle.And it is the detection of tumor cell proliferative activity indicator.It can be used as a classic prognostic marker. Ki-67proteins are now widely on the application, but in cancer research, especially in the prognosis and treatment of laryngeal tumors of research in recent years gradually been reported.
In this study, expression of cyclin-D1and Ki-67in laryngeal squamous cell carcinoma and adjacent tissues, throat normal squamous epithelial tissue of laryngeal cancer are detected in order to provide a reliable molecular markers for early diagnosis and prognosis of laryngeal cancer.
Objective
To study the cell cycle protein D1and cell proliferation antigen markers Ki-67in laryngeal cancer tissue of the expression and the clinical, pathological, the relationship between the two groups as well as the correlation of expression.
Methods
92cases of laryngeal cancer patients after radical surgery sacrificing the specimens from January2000to August2005, including78cases of male,14cases of female. Aged36to79years old, average55.4years old. The tumor:43cases of supraglottic,46cases of glottic and subglottic, three cases. According to the1997International Anti-Cancer Association (UICC) TNM type and phased program, the degree of tumor differentiation:well differentiated (Gl),25cases; moderately differentiated (G2),19cases; poorly differentiated (G3),48cases; TNM clinical stage,6cases of stage Ⅰ;15cases Ⅱ;46cases Ⅲ; Ⅳ of25cases. The immunohistochemical SP staining method is used to detect the expression of cyclin D1and Ki-67protein, with PBS instead of primary antibody as a negative control, and compare the expression of these gene protein in the normal larynx squamous (control group1), laryngeal squamous cell carcinoma,laryngeal tissues (control group2).To study the relationship between the expression of cyclin D1and Ki-67and clinical pathological features and its correlation. The statistical software SPSS12.0statistics. Throat cancer organizations, tumor tissue adjacent group and control group, and between the measurement data to (s) says that on one-way ANOVA method in overall comparison, t test between groups do more, Spearman rank correlation analysis and tests significant level take α=0.05, P<0.05for differences with a statistical significance.
Results
(1) in92tumor samples60patients (65.2%) were observed the cell cycle protein Dl expression, and in the control group1and the group2, and the detection rate were3%(3/100) and13.1%(12/92), three groups for the cell cycle protein D1detection rate with significant difference (P<0.05), and further testing is found in different stages, the laryngeal cancer cells, the organization cell cycle protein D1expression and the clinical stages of the relevance of statistically significant (P=0.029). However, was found in cell cycle protein expression and other variables D1(gender, age, cancer stage, organization, anatomy, part differentiation smoking history, drinking histories) no statistical correlation.
(2) Ki-67immunohistochemical detection results:five element positive signal main role in cytoplasm, as of different thicknesses of palm or brown particles. Normal squamous organization has not seen the throat expression, laryngeal cancer adjacent tissues with a small amount of expression,4cases (4.34%) of the thyroid gland tumor is weak positive expression. And according to the results, Ki-67excessive expression and tumors of patients of gender, age, high differentiation, anatomy site, organization differentiation, smoking history and drinking history is no statistical correlation (see table2). Ki-67expression and lymph node state has a significantly related (P=0.025).
(3) Ki-67expression may and patient surial rates have relevance, Ki-67expression and survival time was statistically relevant (P=0.457). In addition, the cell cycle protein D1surial for patients with expression and the statistical correlation (P=0.0004),
(4) in the Cyclin D1positive oer-expressed in57throat breast cancer organization,41cases Ki-67positive expression. In Ki-67positive low-grade expression of51patients had35patients Cyclin Dl positive low-grade express, the spearman rank related inspection both in the throat papilloma group expression is a significant positive correlation (r=0.620, P<0.001).
Conclusions
(1) Cyclin D1expression in laryngeal squamous cell carcinoma is high.There is a significant relationship with clinical stage and prognosis of the patients. Those with low expression of Cyclin D1have a higher survival rate. Cyclin D1can be used as a laryngeal squamous cell carcinoma postoperative monitoring indicators, and provide a new target for the treatment of laryngeal squamous cell carcinoma.
(2) Ki-67is also expressed in laryngeal squamous cell carcinoma tissues. There is a significant relationship between the expression and neck lymph node metastasis of the patients.Ki-67expression can be used as a lymph node metastasis monitoring indicators of the patients with laryngeal squamous cell carcinoma.
(3) Cyclin D1and Ki-67expression in laryngeal squamous cell carcinoma has a significantly positive correlation. They can be used as a monitoring indicators of lymphnode metastasis and prognosis of patients with laryngeal squamous cell carcinoma after operation.
引文
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