引进肉用种羊和蒙古羊种间抗病性的比较研究——不同品种羊甲状腺激素、硒、谷胱甘肽过氧化物酶关系的研究
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摘要
本研究采用放射免疫法、可见光分光光度法、荧光分光光度法和流式细胞术对多赛特肉羊、德克赛尔肉羊和蒙古羊三个品种，每个品种分羔羊、育成羊、成年羊三组，每组各30只，共270只羊的血清、被毛以及抗凝血样品进行了促甲状腺激素、甲状腺激素、微量元素硒、谷胱甘肽过氧化物酶和外周血T淋巴细胞（CD2）和单核巨噬细胞（CD14）的测定。
结果显示，蒙古羊血清促甲状腺激素和甲状腺激素显著高于德克赛尔肉羊和多赛特肉羊（P＜0.05）；蒙古羊血清和被毛Se含量显著高于德克赛尔肉羊和多赛特肉羊（P＜0.05）；蒙古羊血清GSH-Px活性显著高于德克赛尔肉羊和多赛特肉羊（P＜0.05）；蒙古羊外周血T淋巴细胞（CD2）、单核巨噬细胞（CD14）显著高于德克赛尔肉羊和多赛特肉羊（P＜0.05）。同时，发现机体血清Se水平与甲状腺激素水平呈正相关。
本实验研究证明了在同等饲养管理条件下，蒙古羊被毛Se含量、血清Se含量以及甲状腺激素、促甲状腺激素、谷胱甘肽过氧化物酶和外周血T淋巴细胞（CD2）、单核巨噬细胞（CD14）都显著高于德克赛尔肉羊和多赛特肉羊，而这些指标的差异可能就是德克赛尔肉羊和多赛特肉羊抗病性能低于蒙古羊的原因之一。
Three breeds of sheep, Poll Dorset, Texel Mutton Sheep and Mongolian Sheep were used in this study. Each kind of sheep was divided into three groups that is lamb group, young group and adult group, 30 sheep per group. Thyroid hormone, Thyroid-Stimulating hormone, Selenium, Glutathione peroxidase and peripheral blood T lymphocyte (CD2) and Mononuclear Macrophage (CD14) were determined by using Radioimmunoassay (RIA), Visible Spectrophotofluorometry,Fluorospectrophotomery and Flow Cytometer Technique respectively from the serum samples, wool samples and anticoagulate samples.
The results showed that the levels of Thyroid hormone and Thyroid-Stimulating hormone in serum samples of Mongolian Sheep were significantly higher than those of Poll Dorset and Texel Mutton Sheep（P＜0.05）. The levels of selenium in serum and wool samples of Mongolian Sheep were significantly higher than those of Poll Dorset and Texel Mutton Sheep（P＜0.05）. The activity of Glutathione peroxidase (GSH-Px) in serum samples of Mongolian Sheep was significantly higher than those of Poll Dorset and Texel Mutton Sheep（P＜0.05）.The levels of peripheral blood T lymphocytes(CD2) and Mononuclear Macrophage (CD14) in blood samples of Mongolian Sheep were significantly higher than those of Poll Dorset and Texel Mutton Sheep（P＜0.05）. Additionally, there existed a positive correlation between serum selenium level and thyroid hormone level.
The experiment proved that all the levels of selenium in wool and serum,Thyroid hormone, Thyroid-stimulating hormone, GSH-Px in serum and peripheral blood T lymphocyte (CD2) and Mononuclear Macrophage ( CD14) of Mongolian Sheep were significantly higher than those of Poll Dorset and Texel Mutton Sheep（P＜0.05）With the same conditions of breeding and management ,the differences of these parameters were maybe one of the reasons that the disease resisitivity of Poll Dorset, Texel Mutton Sheep are lower than that of Mongolian Sheep.
结果显示，蒙古羊血清促甲状腺激素和甲状腺激素显著高于德克赛尔肉羊和多赛特肉羊（P＜0.05）；蒙古羊血清和被毛Se含量显著高于德克赛尔肉羊和多赛特肉羊（P＜0.05）；蒙古羊血清GSH-Px活性显著高于德克赛尔肉羊和多赛特肉羊（P＜0.05）；蒙古羊外周血T淋巴细胞（CD2）、单核巨噬细胞（CD14）显著高于德克赛尔肉羊和多赛特肉羊（P＜0.05）。同时，发现机体血清Se水平与甲状腺激素水平呈正相关。
本实验研究证明了在同等饲养管理条件下，蒙古羊被毛Se含量、血清Se含量以及甲状腺激素、促甲状腺激素、谷胱甘肽过氧化物酶和外周血T淋巴细胞（CD2）、单核巨噬细胞（CD14）都显著高于德克赛尔肉羊和多赛特肉羊，而这些指标的差异可能就是德克赛尔肉羊和多赛特肉羊抗病性能低于蒙古羊的原因之一。
Three breeds of sheep, Poll Dorset, Texel Mutton Sheep and Mongolian Sheep were used in this study. Each kind of sheep was divided into three groups that is lamb group, young group and adult group, 30 sheep per group. Thyroid hormone, Thyroid-Stimulating hormone, Selenium, Glutathione peroxidase and peripheral blood T lymphocyte (CD2) and Mononuclear Macrophage (CD14) were determined by using Radioimmunoassay (RIA), Visible Spectrophotofluorometry,Fluorospectrophotomery and Flow Cytometer Technique respectively from the serum samples, wool samples and anticoagulate samples.
The results showed that the levels of Thyroid hormone and Thyroid-Stimulating hormone in serum samples of Mongolian Sheep were significantly higher than those of Poll Dorset and Texel Mutton Sheep（P＜0.05）. The levels of selenium in serum and wool samples of Mongolian Sheep were significantly higher than those of Poll Dorset and Texel Mutton Sheep（P＜0.05）. The activity of Glutathione peroxidase (GSH-Px) in serum samples of Mongolian Sheep was significantly higher than those of Poll Dorset and Texel Mutton Sheep（P＜0.05）.The levels of peripheral blood T lymphocytes(CD2) and Mononuclear Macrophage (CD14) in blood samples of Mongolian Sheep were significantly higher than those of Poll Dorset and Texel Mutton Sheep（P＜0.05）. Additionally, there existed a positive correlation between serum selenium level and thyroid hormone level.
The experiment proved that all the levels of selenium in wool and serum,Thyroid hormone, Thyroid-stimulating hormone, GSH-Px in serum and peripheral blood T lymphocyte (CD2) and Mononuclear Macrophage ( CD14) of Mongolian Sheep were significantly higher than those of Poll Dorset and Texel Mutton Sheep（P＜0.05）With the same conditions of breeding and management ,the differences of these parameters were maybe one of the reasons that the disease resisitivity of Poll Dorset, Texel Mutton Sheep are lower than that of Mongolian Sheep.
引文
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