摘要
从豆科甘草属植物刺果甘草(Glycyrrhiza pallidiflora Maxim)根
及根茎中分出17个化合物(Ⅰ~ⅩⅦ),根据理化性质和UV、IR、EIMS、
~1HNMR、~(13)CNMR及DEPT等波谱数据确定了其中13个化合物的结
构,分别为:后莫紫檀素(Homopterocarpin,Ⅲ)、5α-豆甾-3,6-二
酮(5.α-Stigmastane-3,6-dione,Ⅴ)、β-谷甾醇(β-Sitosterol,
Ⅵ)、4',7-二甲氧基异黄酮(4',7-Dimethoxyisoflavone,Ⅶ)、白
桦脂酸(Betulinic acid,Ⅷ)、美迪紫檀素(Medicarpin,Ⅸ)、异
光甘草酚(Isoglabrol,Ⅺ)、10-甲氧基美迪紫檀素
(10-Methoxy-medicarpin Ⅻ)、9-甲氧基香豆雌酚
(9-Methoxycoumestrol,ⅩⅢ),21α-hydroxy-3-oxo-oleana-11,13
(18)-diene-29-oic acid(ⅩⅣ),芒丙花素(Formononetin,ⅩⅤ)、
马其顿酸(macedonic acid ⅩⅥ)、胡萝卜甙(Daucosterol,ⅩⅦ)。
其中ⅩⅣ为一新化合物,Ⅴ和Ⅻ为甘草属中首次分得,Ⅷ,ⅩⅦ为首
次自该植物中分得。
采用小鼠体内接种实体HepA瘤株,观察了后莫紫檀素、美迪紫
檀素等7个单体对HepA实体瘤的抑制作用,结果发现,其中5个单
体有较强的活性(抑瘤>40%),对其深入评价工作正在开展。
Seventeen compounds were isolated from roots of Glycyrrhiza pallidiflora Maxim, a Chinese folk medicine .Among them, thirteen compounds' structures were established as (-) -6aR, 11 aR-dihydro-3,9-dimethoxy-6H-benzofuro [3,2c] [l]-benzopyran (homopterocarpin III) ,5.alpha.-stigmastane-3,6-dione (V),stigmast-5-en-3 P -ol O
-Si tosterol VI), 7-methoxy-3-(4methoxypheny1)-4H-benzopyran 4-one (4', 7-dimethoxyisoflavone W) , 3 ?-hydroxy-lup-20(29)-en-28-oic acid (betulinic acid VI) , (-)-6aR,llaR-dihydro-3-hydroxy-9-methoxy-6H-benzofuro[3,2c] [1] -benzopyran(medicarpin IX), (s) 2,3- dihydro-7- hydroxy-2-(4-hydroxyphenyl)-6, 8-bis-(3-methyl-2-butenyl)- 4H-benzopyran-4-one (isoglabrol XI) , (-)-6aR,naRKiihydro-3-hydroxy-9,l(klimethoxy-6H-tenzofuro[3,2c] [1]-benziopyran (10-methoxy-medicarpin XH) ,3-hydroxy-9-methoxy-6H-benzofuro[3,2c][l]benzopyran-6-one (9-methoxycoumestrol XIII), 21 a -hydroxy-3-oxo-oleana-ll, 13(18)-diene-29-oic acid (XIV), 7-hydroxy-3~ (4-methoxyphenyl)- 4H-benzopyran-4-one (formononetin XV), 3 ^ , 21 a-dihydroxy-oleana-11, 13(18)
-diene-29-oic acid (macedonic acid XVI) and stigmast-5-en-3 3
-D-glucopyranoside (daucosterol XVII) respectively,byspectroscopic methods. Of them, XIV was a new oleanane triterpenoid, V and Xfl were firstly reported in Glycyrrhiza genus, and other tow compounds , Vffl and XVfl were firstly founded in this plant.
The antiHepA tumour effects were observed in mice administrated seven compounds of above mentioned, five of them showed significant antiHepA activity . Further investigations are now in progress in our laboratory.
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