新疆红枣化学成分与抗氧化活性的研究
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摘要
目的：通过对新疆不同产地红枣化学成分及其抗氧化作用的研究,为红枣的质量控制提供更加稳定、可靠的方法,为红枣的临床用药、食品加工等提供一定的参考。方法：利用分光光度法分别测定红枣中总多酚、总黄酮的含量；采用高效液相色谱法(HPLC法)测定芦丁、齐墩果酸、熊果酸的含量,高分离度快速液相色谱法(RRLC法)测定环磷酸腺苷(cAMP)的含量；利用微波消解-火焰原子吸收法测定金属元素的含量；利用正交试验法对红枣总黄酮的提取工艺进行考察；研究大孔吸附树脂法分离纯化红枣总黄酮的工艺；应用3种抗氧化模型(清除DPPH自由基、羟基自由基以及超氧化物歧化酶(SOD)),对红枣水提取物进行抗氧化活性的测定。结果：6种红枣中均含有总多酚、总黄酮,且含量具有一定程度的差异；芦丁、齐墩果酸、熊果酸及环磷酸腺苷的含量均存在一定的差异；测得红枣中8种金属元素的含量；利用正交试验法优选红枣总黄酮的提取工艺为12倍量70%乙醇回流提取2次,每次1h；D101型树脂具有良好的静态和动态吸附性能。抗氧化实验证明红枣水提取物具有良好的抗氧化活性。结论：本课题对新疆6种不同品种红枣的总多酚、总黄酮、芦丁、齐墩果酸、熊果酸、环磷酸腺苷及金属元素的含量进行了测定,筛选优化红枣总黄酮的提取和分离纯化的工艺参数,并对红枣的水提物进行体外抗氧化实验,为红枣建立科学的质量标准和进一步研究提供理论依据。
Objective:To study chemical composition and antioxidant activity of six cultivars of jujubae fructus from different areas in Xinjiang province, provide a more stable and reliable method for quality control, and provide a reference for clinical medicine and food processing. Methods:Total flavonoids and Polyphenolic contents were measured using spectrophotometry; rutin, oleanolic acid, ursolic acid contents were measured using high performance liquid chromatography (HPLC method), cyclic adenosine monophosphate (cAMP) contents were measured using Rapid Resolution Liquid Chromatography (RRLC); to determine metal elements by Microwave Digestion-Flame Atomic Absorption Spectrometry(FAAS); to optimize the extract technology of total flavonoids from jujubae fructus; to study Separation and purification process of total flavonoids from jujubae fructus using macroporous adsorption resin; utilise three types of antioxidant activity models (clear DPPH free radical, hydroxide radical and SOD active effect) do antioxidant activity screening to water extracts of jujubae fructus. Results:There were differents levels of total flavonoids and polyphenolic contents in six cultivars of jujubae fructus; the contents of rutin, oleanolic acid, ursolic acid and cyclic adenosine monophosphate in six cultivars of jujubae fructus in XinJiang existed obviouse difference; determined metal elements contents in six cultivars of jujubae fructus; through 2 times of refluxing with 70% alcohol, of which the amount was 12 times of sample, and 1h for each refluxing, the best extraction efficiency was obtained. experimental results of the static and dynamic adsorption-desorption experiments show that D101 resin possesses higher absorption and desorption capacity. The results of the present work indicate that the water extracts from six cultivars of jujubae fructus had different levels of antioxidant activity. Conclusion: The study determined contents of total polyphenolic, total flavonoids, rutin, oleanolic acid, ursolic acid, cyclic adenosine monophosphate and metal elements in six cultivars of jujubae fructus, optimized the extract technology of total flavonoids and studied separation and purification process of total flavonoids from jujubae fructus using macroporous adsorption resin, to study antioxidative actions of water extracts from six cultivars of jujubae fructus in vitro, offered theory evidences for quality control and further study of jujubae fructus.
Objective:To study chemical composition and antioxidant activity of six cultivars of jujubae fructus from different areas in Xinjiang province, provide a more stable and reliable method for quality control, and provide a reference for clinical medicine and food processing. Methods:Total flavonoids and Polyphenolic contents were measured using spectrophotometry; rutin, oleanolic acid, ursolic acid contents were measured using high performance liquid chromatography (HPLC method), cyclic adenosine monophosphate (cAMP) contents were measured using Rapid Resolution Liquid Chromatography (RRLC); to determine metal elements by Microwave Digestion-Flame Atomic Absorption Spectrometry(FAAS); to optimize the extract technology of total flavonoids from jujubae fructus; to study Separation and purification process of total flavonoids from jujubae fructus using macroporous adsorption resin; utilise three types of antioxidant activity models (clear DPPH free radical, hydroxide radical and SOD active effect) do antioxidant activity screening to water extracts of jujubae fructus. Results:There were differents levels of total flavonoids and polyphenolic contents in six cultivars of jujubae fructus; the contents of rutin, oleanolic acid, ursolic acid and cyclic adenosine monophosphate in six cultivars of jujubae fructus in XinJiang existed obviouse difference; determined metal elements contents in six cultivars of jujubae fructus; through 2 times of refluxing with 70% alcohol, of which the amount was 12 times of sample, and 1h for each refluxing, the best extraction efficiency was obtained. experimental results of the static and dynamic adsorption-desorption experiments show that D101 resin possesses higher absorption and desorption capacity. The results of the present work indicate that the water extracts from six cultivars of jujubae fructus had different levels of antioxidant activity. Conclusion: The study determined contents of total polyphenolic, total flavonoids, rutin, oleanolic acid, ursolic acid, cyclic adenosine monophosphate and metal elements in six cultivars of jujubae fructus, optimized the extract technology of total flavonoids and studied separation and purification process of total flavonoids from jujubae fructus using macroporous adsorption resin, to study antioxidative actions of water extracts from six cultivars of jujubae fructus in vitro, offered theory evidences for quality control and further study of jujubae fructus.
引文
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