中草药复方制剂对公猪精液品质的影响研究
|
摘要
猪人工授精技术近年来推广迅速,已经成为科学养猪、实现荞猪生产现代化的重要手段,是培育种猪和生产商品猪的最简单有效的方法。公猪的精液品质是影响人工授精技术推广最重要的一个因素,研究如何提高公猪精液品质尤其是中草药对精液品质影响的研究日趋热门。
研究目的：
1.对溆浦县生猪产业和猪人工授精的发展形成初步的认识,为进一步推广人工授精技术提供基础。
2.结合溆浦县养猪业和人工授精的状况,研究中草药复方制剂对公猪精液品质的影响。
研究内容和方法：
1.通过普遍调查、典型调查、个案调查、抽样调查以及文献调查、问卷调查、访问调查、观察调查等方法调查溆浦县养猪业状况和猪人工授精的发展推广现状,提出相应的对策和措施,为溆浦县生猪产业化发展提供科学依据。
2.中草药复方制剂对公猪精液品质的影响：
选取条件相近的健康大约克种猪27头,分为对照组,低剂量组,高剂量组,每组7个重复。对照组：采用基础日粮；低剂量试验组：采用基础日粮+2%中草药复方添加剂；高剂量试验组：采用基础日粮+3%中草药复方添加剂,试验期为2个月,每组隔4天采精1次,并测定采精持续时间、采精量、精子密度、精子活力、精子畸形率等指标,每隔10天对各组试验猪耳缘静脉采血,测定血清中FSH、LH和T的含量。
研究结果：
1.溆浦县养猪业和人工授精技术的发展势头良好,极具发展潜力。但在生猪行业基础薄弱、产业化程度不高、结构不合理,在人工授精技术推广方面存在技术人员水平不高、发展资金不足、硬件设施落后等问题。
2.中草药复方制剂对公猪精液品质影响试验的结果表明,低剂量组的采精量、采精持续时间、精子密度,血清中FSH、LH、T含量与对照组相比,均有显著的提高(p<0.05)；与对照组相比高剂量组的采精持续时间、精子活力和血液中LH、T显著提高(p<0.05),采精量和血液中FSH含量有极显著差异(p<0.01),而低剂量组和高剂量组的精子畸形率与对照组相比都有所降低。
结论：
1.溆浦县目前的养猪产业发展势头良好,人工授精技术推广正在迅速展开,针对实践过程中发现的一些问题,采用新的政策来推动其发展。在进一步解决目前所面临的问题后,其发展前景将更加广阔。
2.中草药复方制剂能够显著提高公猪精液采集持续时间、精液量、精子密度、精子活力,降低精子畸形率,增加血清中FSH、LH、T的含量,其中以添加3%中草药复方制剂试验组改善和提高精液品质效果明显,为以后的推广应用奠定了基础。
Pig artificial insemination technology rapidly develop and quickly spreads throughout in recent years, it has become scientific pig production and realize the important means of modernization of the swine, which the most simple and effective breeding methods of training and production commercial audubon. The boar's sperm quality is the most important influence artificial insemination of a factor. In recent years the Chinese herbal medicine research will become increasingly popular. So there are two aspects of this experiment to study Chinese herbal medicine to the influence of the boar semen quality.
Research objectives
1.To pig industry and the development of artificial insemination pigs have a preliminary understanding in Xupu town, and the next step of the artificial insemination provide basis.
2.Combined with both the pig industry and the status of the artificial insemination in Xupu town, which to study Chinese herbal compound preparations for the influence of the boar semen quality.
Research content and methods
1. Surveying pig breeding condition in Xupu town, and the development of artificial insemination promotion present situation, so bring forward relevant counter measures and suggestion, which continue to promote the development of the whole Xupu pig breeding.
2.Chinese herbal medicine compound preparations is the influence of the boar semen quality. Select the health of similar condition about Large White 27 heads, which divided into the control group, the low dose group, the high dose group, and seven repeat of each group. The control group, basal diet; the low dose group,basal diet+2% Chinese herbal medicine compound additives; the high dose group, basal diet+3% Chinese herbal medicine compound additives. There is trial period for two months, and after four days of every group is collected semen, then the determination of length of semen-collection time, fresh semen volume, sperm density, sperm activity, abnormal sperm rate and so on. Every 10 days, veterinarians pick discolored ears marginal vein blood of each experiment group and measure the content of FSH, LH and T in the semen.
Research results
1.The rapid development of both the pig industry and artificial insemination in Xupu town, which has tremendous development potential. Although there are some problems to be solved, the development prospects will be very good.
2.The experiment results show that compared with control group, the length of semen-collection time, fresh semen volume, sperm density, and the content of FSH,LH and T in the serum of the low dose group are improved significantly (P<0.05).The length of semen-collection time, sperm activity and the content of LH and T in the serum of the high dose group has significant difference compared with the control group(P<0.05), fresh semen volume and the content of FSH in the serum of the high dose group has very significant difference compared with the control group(P<0.01). And abnormal sperm rate of low dose group and high dose group compared with the control group are reduced.
Conclusion
1.Combined with the current hog industry and artificial insemination situation in Xupu town, and according to some problems, which put forward the constructive opinions and by using the new policies to promote its development.
2.Chinese herbal medicine compound preparations can improve boar sperm collection duration, fresh semen volume, sperm density and sperm activity, reduce abnormal sperm rate and increase the content of FSH, LH and T in the sperm. So it can improve and enhance the quality of semen, in the same time lay the foundation of popularization and application.
研究目的：
1.对溆浦县生猪产业和猪人工授精的发展形成初步的认识,为进一步推广人工授精技术提供基础。
2.结合溆浦县养猪业和人工授精的状况,研究中草药复方制剂对公猪精液品质的影响。
研究内容和方法：
1.通过普遍调查、典型调查、个案调查、抽样调查以及文献调查、问卷调查、访问调查、观察调查等方法调查溆浦县养猪业状况和猪人工授精的发展推广现状,提出相应的对策和措施,为溆浦县生猪产业化发展提供科学依据。
2.中草药复方制剂对公猪精液品质的影响：
选取条件相近的健康大约克种猪27头,分为对照组,低剂量组,高剂量组,每组7个重复。对照组：采用基础日粮；低剂量试验组：采用基础日粮+2%中草药复方添加剂；高剂量试验组：采用基础日粮+3%中草药复方添加剂,试验期为2个月,每组隔4天采精1次,并测定采精持续时间、采精量、精子密度、精子活力、精子畸形率等指标,每隔10天对各组试验猪耳缘静脉采血,测定血清中FSH、LH和T的含量。
研究结果：
1.溆浦县养猪业和人工授精技术的发展势头良好,极具发展潜力。但在生猪行业基础薄弱、产业化程度不高、结构不合理,在人工授精技术推广方面存在技术人员水平不高、发展资金不足、硬件设施落后等问题。
2.中草药复方制剂对公猪精液品质影响试验的结果表明,低剂量组的采精量、采精持续时间、精子密度,血清中FSH、LH、T含量与对照组相比,均有显著的提高(p<0.05)；与对照组相比高剂量组的采精持续时间、精子活力和血液中LH、T显著提高(p<0.05),采精量和血液中FSH含量有极显著差异(p<0.01),而低剂量组和高剂量组的精子畸形率与对照组相比都有所降低。
结论：
1.溆浦县目前的养猪产业发展势头良好,人工授精技术推广正在迅速展开,针对实践过程中发现的一些问题,采用新的政策来推动其发展。在进一步解决目前所面临的问题后,其发展前景将更加广阔。
2.中草药复方制剂能够显著提高公猪精液采集持续时间、精液量、精子密度、精子活力,降低精子畸形率,增加血清中FSH、LH、T的含量,其中以添加3%中草药复方制剂试验组改善和提高精液品质效果明显,为以后的推广应用奠定了基础。
Pig artificial insemination technology rapidly develop and quickly spreads throughout in recent years, it has become scientific pig production and realize the important means of modernization of the swine, which the most simple and effective breeding methods of training and production commercial audubon. The boar's sperm quality is the most important influence artificial insemination of a factor. In recent years the Chinese herbal medicine research will become increasingly popular. So there are two aspects of this experiment to study Chinese herbal medicine to the influence of the boar semen quality.
Research objectives
1.To pig industry and the development of artificial insemination pigs have a preliminary understanding in Xupu town, and the next step of the artificial insemination provide basis.
2.Combined with both the pig industry and the status of the artificial insemination in Xupu town, which to study Chinese herbal compound preparations for the influence of the boar semen quality.
Research content and methods
1. Surveying pig breeding condition in Xupu town, and the development of artificial insemination promotion present situation, so bring forward relevant counter measures and suggestion, which continue to promote the development of the whole Xupu pig breeding.
2.Chinese herbal medicine compound preparations is the influence of the boar semen quality. Select the health of similar condition about Large White 27 heads, which divided into the control group, the low dose group, the high dose group, and seven repeat of each group. The control group, basal diet; the low dose group,basal diet+2% Chinese herbal medicine compound additives; the high dose group, basal diet+3% Chinese herbal medicine compound additives. There is trial period for two months, and after four days of every group is collected semen, then the determination of length of semen-collection time, fresh semen volume, sperm density, sperm activity, abnormal sperm rate and so on. Every 10 days, veterinarians pick discolored ears marginal vein blood of each experiment group and measure the content of FSH, LH and T in the semen.
Research results
1.The rapid development of both the pig industry and artificial insemination in Xupu town, which has tremendous development potential. Although there are some problems to be solved, the development prospects will be very good.
2.The experiment results show that compared with control group, the length of semen-collection time, fresh semen volume, sperm density, and the content of FSH,LH and T in the serum of the low dose group are improved significantly (P<0.05).The length of semen-collection time, sperm activity and the content of LH and T in the serum of the high dose group has significant difference compared with the control group(P<0.05), fresh semen volume and the content of FSH in the serum of the high dose group has very significant difference compared with the control group(P<0.01). And abnormal sperm rate of low dose group and high dose group compared with the control group are reduced.
Conclusion
1.Combined with the current hog industry and artificial insemination situation in Xupu town, and according to some problems, which put forward the constructive opinions and by using the new policies to promote its development.
2.Chinese herbal medicine compound preparations can improve boar sperm collection duration, fresh semen volume, sperm density and sperm activity, reduce abnormal sperm rate and increase the content of FSH, LH and T in the sperm. So it can improve and enhance the quality of semen, in the same time lay the foundation of popularization and application.
引文
[1]张守全.猪人工授精[J].养猪,2006,6：9-11.
[2]刘国世,王剑,薛振华,等.国外猪人工授精技术研究进展[J].猪业科学,2007,7：16-19.
[3]郑不留.中国猪品种志[M].上海科学技术出版社,1986：45-66.
[4]韩陆奇.猪[M].北京：中国商业出版社,1982：103-112.
[5]凌泽继,伏彭辉,张家庆,等.中国猪人工授精的研究及应用状况[J].养猪,2008,6：9-11.
[6]张晶.上世纪50年代广西猪人工授精技术的研究与应用[J].2007,5：23-25.
[7]穆建平,朱林跟.浅谈人工授精技术[J].中国畜禽种业,2011,3：75-76.
[8]王俊勤.国家生猪人工授精项目现状与发展[J].“格拉姆杯“提高母猪年生产力及第四届全国猪人工授精关键技术眼讨论会.
[9]彭永涛.生猪人工授精技术应用的利弊及发展趋势[J].2009,(8)：85.
[10]安宁.猪人工授精技术推广思考[J].陕西农业科学,2011,4：121-125.
[11]陈志明,钟辉.浅谈猪人工授精技术的推广[J].动物饲养,2007,3：16.
[12]金从福,朱保国,郭仁龙,等.猪人工授精的发展与优点[J].畜禽业,2010,259(11)：55.
[13]李信.浅谈猪的人工授精技术[J].畜禽养殖,2011,3：44-45.
[14]胡疆峤.影响人工授精的因素与对策[J].家畜养殖,2011,1：16-17.
[15]张美荣,寸彦铭,张政椿,等.提高猪人工授精受胎率的技术措施[J].养殖与饲料,2011,3：13-14.
[16]呼合武.影响人工授精效果的几个因素[J].河南畜牧兽医,2005,8(26)：13-14.
[17]李香洲,王铁柱,荣柏庆,等.影响猪人工授精效果的主要原因[J].黑龙江动物繁殖,2010,18(1)：32-34.
[18]付云超,薛琳琳.影响猪人工授精效果的原因分析[J].黑龙江畜牧兽医职业学院学报,2010,9(12)：74-79.
[19]冯晓毅,王淑霞.母猪人工授精受胎率低得原因[J].黑龙江动物繁殖,18(1)：32-34.
[20]吴正杰,江美良.生猪人工授精的常见问题及解决方案[J].生产管理,2010,4：37-40.
[21]潘先汉.种公猪的饲养管理与合理使用[J].贵州畜牧兽医,2001,25(6)：34.
[22]易宏辉,雷勇,陈志军,等.影响公猪精液品质的主要因素概述[J].湖南畜牧兽医,2011,162(2)：1-4.
[23]周春宝,汪劲能,陶勇,等.不同季节对杜洛克公猪精液品质的影响[J].黑龙江动物繁殖,2006,14(4)：5-6.
[24]蒋如明,饶秋亮,黎姓毅.在中国南方条件下在不同月份中瘦肉型公猪精液品质的变化[J]. 第十二届全国动物反之学术讨论会论文集.
[25]方艳玲,胡仲达.高温对猪生产性能的影响[J].安徽农学通报,2009,9(15)：194-195.
[26]邵良平,黄葳,薛永钦.高温季节公猪舍内温度对公猪精液品质的影响[J].福建省第三届猪病学术研讨会论文集.
[27]胡金平,曾乐平,李菊峰,等.气温对种猪繁殖性能的影响[J].家畜生态学报,2008,29(3)：33-36.
[28]陈赞谋,黄冰.气候因子对母猪繁殖性能的影响[J].家畜生态,2002,5：9.
[29]田萍,李衡良,姚武群.湿帘降温公猪舍环境状况的测定和评价[J].2002,(4)：38.
[30]廖文良.热应激对猪只的危害及防治措施[J].兽医临床,2010,(5)：102.
[31]崔久慧,王凤丽,曲永生.如何减轻高温对公猪的不良影响[J].畜牧兽医科技信息,2006,10：61.
[32]王吴剡.种公猪夏季高温饲养管理对策[J].畜牧兽医科技信息,2010,7：89.
[33]黄健,杨飞云,刘作华,等.能量和蛋白质对公猪精液品质和血液指标的影响[J].饲料工业,2008,29(19)：14-17.
[34]]王金全.日粮能量蛋白水平对种公猪繁殖性能的影吲[J].养猪,2000,(4)：12-15.
[35]杨飞云,游小燕,刘作华,等.不同蛋白质水平下中公猪精液品质与血液圣湖指标的相关性研究[J].营养饲料,2008,44(11)：18-20.
[36]沈培庆.日粮中添加赖氨酸对提高公猪精液品质的效果[J].畜牧与兽医,1997,6：257-258.
[37]李雪梅,曾饶琼,易宗荣,等.维生素A饲喂量对种公猪精液品质的影响[J].安徽农业科学,2008,36(33)：14540-14544.
[38]李有超.维生素D在动物生产中的研究与应用[J].畜禽业,2006(8)：20-23.
[39]李德发.维生素D对断奶仔猪生产性能和免疫机能的影响[J].中国农业大学学报,2001(5)：87-94.
[40]梁明振,卢克焕,李琼华.饲粮维生素E水平对公猪繁殖及精浆营养生化参数的影响[J].养猪,2003,7-9.
[41]干怀禹.日粮维生素E水平对种公猪精液品质的影响[J].营养与饲料,2010,2：50-53.
[42]何若刚,杨金宝,张文明,等.酵母锌对种公猪繁殖及精清激素参数的影响[J].畜牧与兽医,2008,40(5)：50-52.
[43]Uu C H,etal. Preliminary studies on influence of selenium to the developments of fenital organs and spermatogenesis of infancy boars[J]. Acta Vet Zootech Sin,1982(13):73-77.
[44]苏惠龙,李儒曙,贺湘仁,等.富硒益生菌对种公猪精液品质的影响[J].广东农业科 学,2009,7：156-158.
[45]龙翔.复方中苹药添加荆对哺乳母猪泌乳力的影响2002,(6)：45.
[46]张莉,刘作华,宋代军.热应激对中公猪精液品质和血液指标的影响[J].繁殖技术,2008,233(9)：18-20.
[47]杨金宝,唐湘方,何若刚.影响种公猪繁殖性能的营养因素[J].营养与饲料,2008,6：62-63.
[48]陈寒青,金祉宁.中草药饲料添加剂研究进展[J].饲料工业,2002,23(10)：18.23.
[49]李文彬,李维洁,杨程,等.生长肥育猪中草药饲料添加剂[J].中国畜牧杂志,2001,37(6)：42-43.
[50]于明,程波,王景春,等.中草药复方制剂对断奶仔猪生长性能和肠道菌群的影响[J].养猪,2008,6：14-15.
[51]贾宝生,王振,贾永强,等.中草药及其制剂对猪、鸡、羊疾病的免疫调节作用[J].郑州牧业工程高等专科学校校报,2010,30(30)：7-8.
[52]金岭梅,顾惠明,江立方,等.抗热应激中草药饲料添加剂对商品猪生产性能的影响[J].中国饲料,1998(5)：13-14.
[53]汀义金,高仲平,王娜,等.中草药饲料添加剂的研究概况[J].畜牧与饲料科学,2010,31(5)：73-75.
[54]夏新山,袁书林,杨元青,等.中草药添加剂及不同饲粮类型对生长育肥猪血液生化指标的影响[J].动物科学与动物医学,2003,12(20)：38-39.
[55]马广君,张磊,顾文松,等.中药制剂对保育猪生产性能的影响[J].饲料工业,2009,30(18)：29-31.
[2]刘国世,王剑,薛振华,等.国外猪人工授精技术研究进展[J].猪业科学,2007,7：16-19.
[3]郑不留.中国猪品种志[M].上海科学技术出版社,1986：45-66.
[4]韩陆奇.猪[M].北京：中国商业出版社,1982：103-112.
[5]凌泽继,伏彭辉,张家庆,等.中国猪人工授精的研究及应用状况[J].养猪,2008,6：9-11.
[6]张晶.上世纪50年代广西猪人工授精技术的研究与应用[J].2007,5：23-25.
[7]穆建平,朱林跟.浅谈人工授精技术[J].中国畜禽种业,2011,3：75-76.
[8]王俊勤.国家生猪人工授精项目现状与发展[J].“格拉姆杯“提高母猪年生产力及第四届全国猪人工授精关键技术眼讨论会.
[9]彭永涛.生猪人工授精技术应用的利弊及发展趋势[J].2009,(8)：85.
[10]安宁.猪人工授精技术推广思考[J].陕西农业科学,2011,4：121-125.
[11]陈志明,钟辉.浅谈猪人工授精技术的推广[J].动物饲养,2007,3：16.
[12]金从福,朱保国,郭仁龙,等.猪人工授精的发展与优点[J].畜禽业,2010,259(11)：55.
[13]李信.浅谈猪的人工授精技术[J].畜禽养殖,2011,3：44-45.
[14]胡疆峤.影响人工授精的因素与对策[J].家畜养殖,2011,1：16-17.
[15]张美荣,寸彦铭,张政椿,等.提高猪人工授精受胎率的技术措施[J].养殖与饲料,2011,3：13-14.
[16]呼合武.影响人工授精效果的几个因素[J].河南畜牧兽医,2005,8(26)：13-14.
[17]李香洲,王铁柱,荣柏庆,等.影响猪人工授精效果的主要原因[J].黑龙江动物繁殖,2010,18(1)：32-34.
[18]付云超,薛琳琳.影响猪人工授精效果的原因分析[J].黑龙江畜牧兽医职业学院学报,2010,9(12)：74-79.
[19]冯晓毅,王淑霞.母猪人工授精受胎率低得原因[J].黑龙江动物繁殖,18(1)：32-34.
[20]吴正杰,江美良.生猪人工授精的常见问题及解决方案[J].生产管理,2010,4：37-40.
[21]潘先汉.种公猪的饲养管理与合理使用[J].贵州畜牧兽医,2001,25(6)：34.
[22]易宏辉,雷勇,陈志军,等.影响公猪精液品质的主要因素概述[J].湖南畜牧兽医,2011,162(2)：1-4.
[23]周春宝,汪劲能,陶勇,等.不同季节对杜洛克公猪精液品质的影响[J].黑龙江动物繁殖,2006,14(4)：5-6.
[24]蒋如明,饶秋亮,黎姓毅.在中国南方条件下在不同月份中瘦肉型公猪精液品质的变化[J]. 第十二届全国动物反之学术讨论会论文集.
[25]方艳玲,胡仲达.高温对猪生产性能的影响[J].安徽农学通报,2009,9(15)：194-195.
[26]邵良平,黄葳,薛永钦.高温季节公猪舍内温度对公猪精液品质的影响[J].福建省第三届猪病学术研讨会论文集.
[27]胡金平,曾乐平,李菊峰,等.气温对种猪繁殖性能的影响[J].家畜生态学报,2008,29(3)：33-36.
[28]陈赞谋,黄冰.气候因子对母猪繁殖性能的影响[J].家畜生态,2002,5：9.
[29]田萍,李衡良,姚武群.湿帘降温公猪舍环境状况的测定和评价[J].2002,(4)：38.
[30]廖文良.热应激对猪只的危害及防治措施[J].兽医临床,2010,(5)：102.
[31]崔久慧,王凤丽,曲永生.如何减轻高温对公猪的不良影响[J].畜牧兽医科技信息,2006,10：61.
[32]王吴剡.种公猪夏季高温饲养管理对策[J].畜牧兽医科技信息,2010,7：89.
[33]黄健,杨飞云,刘作华,等.能量和蛋白质对公猪精液品质和血液指标的影响[J].饲料工业,2008,29(19)：14-17.
[34]]王金全.日粮能量蛋白水平对种公猪繁殖性能的影吲[J].养猪,2000,(4)：12-15.
[35]杨飞云,游小燕,刘作华,等.不同蛋白质水平下中公猪精液品质与血液圣湖指标的相关性研究[J].营养饲料,2008,44(11)：18-20.
[36]沈培庆.日粮中添加赖氨酸对提高公猪精液品质的效果[J].畜牧与兽医,1997,6：257-258.
[37]李雪梅,曾饶琼,易宗荣,等.维生素A饲喂量对种公猪精液品质的影响[J].安徽农业科学,2008,36(33)：14540-14544.
[38]李有超.维生素D在动物生产中的研究与应用[J].畜禽业,2006(8)：20-23.
[39]李德发.维生素D对断奶仔猪生产性能和免疫机能的影响[J].中国农业大学学报,2001(5)：87-94.
[40]梁明振,卢克焕,李琼华.饲粮维生素E水平对公猪繁殖及精浆营养生化参数的影响[J].养猪,2003,7-9.
[41]干怀禹.日粮维生素E水平对种公猪精液品质的影响[J].营养与饲料,2010,2：50-53.
[42]何若刚,杨金宝,张文明,等.酵母锌对种公猪繁殖及精清激素参数的影响[J].畜牧与兽医,2008,40(5)：50-52.
[43]Uu C H,etal. Preliminary studies on influence of selenium to the developments of fenital organs and spermatogenesis of infancy boars[J]. Acta Vet Zootech Sin,1982(13):73-77.
[44]苏惠龙,李儒曙,贺湘仁,等.富硒益生菌对种公猪精液品质的影响[J].广东农业科 学,2009,7：156-158.
[45]龙翔.复方中苹药添加荆对哺乳母猪泌乳力的影响2002,(6)：45.
[46]张莉,刘作华,宋代军.热应激对中公猪精液品质和血液指标的影响[J].繁殖技术,2008,233(9)：18-20.
[47]杨金宝,唐湘方,何若刚.影响种公猪繁殖性能的营养因素[J].营养与饲料,2008,6：62-63.
[48]陈寒青,金祉宁.中草药饲料添加剂研究进展[J].饲料工业,2002,23(10)：18.23.
[49]李文彬,李维洁,杨程,等.生长肥育猪中草药饲料添加剂[J].中国畜牧杂志,2001,37(6)：42-43.
[50]于明,程波,王景春,等.中草药复方制剂对断奶仔猪生长性能和肠道菌群的影响[J].养猪,2008,6：14-15.
[51]贾宝生,王振,贾永强,等.中草药及其制剂对猪、鸡、羊疾病的免疫调节作用[J].郑州牧业工程高等专科学校校报,2010,30(30)：7-8.
[52]金岭梅,顾惠明,江立方,等.抗热应激中草药饲料添加剂对商品猪生产性能的影响[J].中国饲料,1998(5)：13-14.
[53]汀义金,高仲平,王娜,等.中草药饲料添加剂的研究概况[J].畜牧与饲料科学,2010,31(5)：73-75.
[54]夏新山,袁书林,杨元青,等.中草药添加剂及不同饲粮类型对生长育肥猪血液生化指标的影响[J].动物科学与动物医学,2003,12(20)：38-39.
[55]马广君,张磊,顾文松,等.中药制剂对保育猪生产性能的影响[J].饲料工业,2009,30(18)：29-31.