中晚熟抗黑腐病青花菜新品种种子生产与高产栽培技术研究
|
摘要
通过自交不亲和系的运用和回交转育的方法得到综合性状优良,且高抗黑腐病的的杂交组合J9806B58-4xB68。该品种已经于2004年通过上海市品种审定委员会审定,审定名为申绿二号。该品种中晚熟,主花球类型,长江中下游地区秋季定植后100d左右达到采收标准,花球高圆形,平均单球重450-500g。花蕾细密,花枝短,小花球排列紧凑,深绿色。球茎充实,不中空。尤其是遇到零下5度低温时,不出现紫球现象。抗黑腐病。该品种与目前生产上主栽的进口品种圣绿相比较,始收期早3-5天,终收期大体相当;而且植株长势强盛,平均单球重494g,比圣绿高4.6%。产量平均为910kg/667m2,比圣绿高8.7%;商品率81.3%以上,与圣绿大体相当。2002-2006年期间,在浙江、上海等地建立示范基地5个,示范面积200余亩,在上海金山建立出口示范基地1000余亩,累计推广3万余亩,实现产值4500万元以上,新增产值500万元以上。
在申绿二号青花菜品种杂交种子生产中,为了实现父母本花期相遇,母本播期为8月27日到9月1日,父本播期延迟25-30天。父母本种植比例为1:1有利于实现种子高产和增加种子的千粒重。申绿二号青花菜新品种在上海地区秋季栽培适宜播种期为7月31日到8月中旬,适期内早播有利于提高产量与品质。栽培密度以2200株/667m2为最佳密度。该品种比较耐瘠薄,忌过量施肥,比常规减少施肥用量20%对(时)不影响单球重和产量以及出口商品率。应用穴盘基质苗栽培,可提早采收4天,产量和品质都优于土壤苗。
通过研究和总结,建立了四套相关的技术规范。包括亲本繁殖技术规范;杂交种子生产的技术规程;工厂化育苗技术规范以及高产高效栽培技术规范。为该品种的推广应用奠定了扎实的基础。
中晚熟抗黑腐病青花菜新品种申绿二号的选育,大大提高了青花菜种子的自给率和商品率,增强了我国青花菜产品在国际市场的竞争力,对于推动上海种源农业发展,提高企业自主创新能力具有多方面重要意义。
The Black-rot resistant hybrid J9806B58-4xB68 with fine integrated traits was produced by the utilization of self-cross sterile lines and back-cross. The hybrid has been identified by Shanghai Identification Committee for New Varieties of Crops, and was given a identified name“Shenglv Erhao”. The hybrid is middle-late matured and its growing period varies from 120 days to 130 days in downstream of Yangtze River area. The top head is its main part of the yield, and its shape looks like high round. The average weightiness per head is 450-500g.The bud is very small ,.uniform and arranged tightly. The flowering branch is short. It’s steak is enrich without empty heart. The hybrid is strong resistant to black-rot disease. Compared with the main variety "Shenglv" ,imported from Japan, Shenglv Erhao grows stronger, matures three-days earlier, and the end harvest time is almost the same . The average weightiness per head of Shenglv Erhao is 494g, that is 4.6% heavier than Shenglv. It’s average yield gets 9101 kg/667m2, that is 8.7% higher than Shenglv. It’s commercial rate gets 81.3%, that is the same as Shenglv. Five demonstrating fields of 15hm2 was established in Zhejiang, Shanghai and other districts. 66hm22 demonstrating land for exporting was established in Jinshan district, Shanghai. 2000hm2 has been popularized to use the hybrid Shenglv Erhao in broccoli cultivation within 2002-2006. The production value gets 45 million Yuan and increased production value gets 5 million Yuan. When the seeds of hybrid of Shenglv Erhao is to be produced, in order to guarantee flowering time of the male parents and female parents to meet, the suitable sowing time of female parent is from 27,Aug to 1,Sep. Sowing time of male parents is recommended delay for 25 days to one month. The optimum ratio of male and female parent should be 1:1. When Shenglv Erhao is utilized to broccoli production, The best sowing date is suggested from 31,July to 20,August. Within this period, earlier sowed, more yield. The hybrid is tolerant to thin field, and Sensitive to over fertilizer. When fertilizer is reduced by 20%, the average weightiness per head and yield per area will not reduce. The optimum density should be 2200/667m2 When tray seedlings were applied, the yield and the quality were better than that seedlings grew from soil were used, and the harvest time is 4 days earlier.
Four technology protocol has been established through researching and summarizing. That includes parents propagation, the production of the seeds of the hybrid, the production of the tray seedlings and the cultivation with high yield and profits. All these have laid solid foundation to extension and utilization for hybrid Shenglv Erhao. The breeding of hybrid Shenglv Erhao with black rot resistant and for exported cultivation, will not also increase self-supply rate and commercial rate significantly, but also increase competitive capacity of the products of broccoli in China. It will play a very important role in promoting the development of the seed agriculture in Shanghai, and in improving self-innovation capacity in agricultural enterprise.
在申绿二号青花菜品种杂交种子生产中,为了实现父母本花期相遇,母本播期为8月27日到9月1日,父本播期延迟25-30天。父母本种植比例为1:1有利于实现种子高产和增加种子的千粒重。申绿二号青花菜新品种在上海地区秋季栽培适宜播种期为7月31日到8月中旬,适期内早播有利于提高产量与品质。栽培密度以2200株/667m2为最佳密度。该品种比较耐瘠薄,忌过量施肥,比常规减少施肥用量20%对(时)不影响单球重和产量以及出口商品率。应用穴盘基质苗栽培,可提早采收4天,产量和品质都优于土壤苗。
通过研究和总结,建立了四套相关的技术规范。包括亲本繁殖技术规范;杂交种子生产的技术规程;工厂化育苗技术规范以及高产高效栽培技术规范。为该品种的推广应用奠定了扎实的基础。
中晚熟抗黑腐病青花菜新品种申绿二号的选育,大大提高了青花菜种子的自给率和商品率,增强了我国青花菜产品在国际市场的竞争力,对于推动上海种源农业发展,提高企业自主创新能力具有多方面重要意义。
The Black-rot resistant hybrid J9806B58-4xB68 with fine integrated traits was produced by the utilization of self-cross sterile lines and back-cross. The hybrid has been identified by Shanghai Identification Committee for New Varieties of Crops, and was given a identified name“Shenglv Erhao”. The hybrid is middle-late matured and its growing period varies from 120 days to 130 days in downstream of Yangtze River area. The top head is its main part of the yield, and its shape looks like high round. The average weightiness per head is 450-500g.The bud is very small ,.uniform and arranged tightly. The flowering branch is short. It’s steak is enrich without empty heart. The hybrid is strong resistant to black-rot disease. Compared with the main variety "Shenglv" ,imported from Japan, Shenglv Erhao grows stronger, matures three-days earlier, and the end harvest time is almost the same . The average weightiness per head of Shenglv Erhao is 494g, that is 4.6% heavier than Shenglv. It’s average yield gets 9101 kg/667m2, that is 8.7% higher than Shenglv. It’s commercial rate gets 81.3%, that is the same as Shenglv. Five demonstrating fields of 15hm2 was established in Zhejiang, Shanghai and other districts. 66hm22 demonstrating land for exporting was established in Jinshan district, Shanghai. 2000hm2 has been popularized to use the hybrid Shenglv Erhao in broccoli cultivation within 2002-2006. The production value gets 45 million Yuan and increased production value gets 5 million Yuan. When the seeds of hybrid of Shenglv Erhao is to be produced, in order to guarantee flowering time of the male parents and female parents to meet, the suitable sowing time of female parent is from 27,Aug to 1,Sep. Sowing time of male parents is recommended delay for 25 days to one month. The optimum ratio of male and female parent should be 1:1. When Shenglv Erhao is utilized to broccoli production, The best sowing date is suggested from 31,July to 20,August. Within this period, earlier sowed, more yield. The hybrid is tolerant to thin field, and Sensitive to over fertilizer. When fertilizer is reduced by 20%, the average weightiness per head and yield per area will not reduce. The optimum density should be 2200/667m2 When tray seedlings were applied, the yield and the quality were better than that seedlings grew from soil were used, and the harvest time is 4 days earlier.
Four technology protocol has been established through researching and summarizing. That includes parents propagation, the production of the seeds of the hybrid, the production of the tray seedlings and the cultivation with high yield and profits. All these have laid solid foundation to extension and utilization for hybrid Shenglv Erhao. The breeding of hybrid Shenglv Erhao with black rot resistant and for exported cultivation, will not also increase self-supply rate and commercial rate significantly, but also increase competitive capacity of the products of broccoli in China. It will play a very important role in promoting the development of the seed agriculture in Shanghai, and in improving self-innovation capacity in agricultural enterprise.
引文
[1] 孙民,西宁地区南运蔬菜-西兰花优质丰产栽培技术,北方园艺,2006,6.
[2] 曹东贺,圆葱与西兰花复种技术,新农业,2006,11.
[3] 牟水元,温室西兰花秋冬栽培技术,农技服务,2006,10.
[4] 李建良, 王慧芬,西兰花主要病虫害及其无公害防治技术,北京农业,2006,9.
[5] 姚学竹,景泰县地膜西兰花无公害栽培技术,甘肃农业科技,2006,9.
[6] 王国升,黄曼华,夏秋反季节西兰花优质高产无公害栽培技术,耕作与栽培,2006,4.
[7] 周玉玲,大棚春青花菜-夏甘蓝-秋番茄一年三种三收模式,西北园艺,2006,5.
[8] 刘威,张云川,不同漂烫温度对西兰花品质指标的影响,农产品加工学刊,2007,1.
[9] 赵志国,支荣英,青花菜无公害栽培技术,西北园艺,蔬菜,2007,1.
[10] 柴守霞,“防癌新秀”西兰花,家庭护士,2007,1.
[11] 陈秋松,西兰花新品--福至二号,农家之友,2006,11.
[12] 谢以泽, 黄新灿,甘蓝·西兰花主要病虫害无害化防治技术的大田控制效果,安徽农业科学, 2006,21.
[13] 高旭春,邹桂花,青花菜四季高产栽培技术,当代蔬菜-2006年8期
[14] 刘振铃,夏庆伟,西兰花一代杂交制种产量低的原因与科学的采种方法,上海蔬菜,2006,3.
[15] 许广祥,马东西兰花生理病害防控措施,新农业,2006,4.
[16] 陈海平,董荷玲,台州市西兰花标准化栽培技术,长江蔬菜,2006,5.
[17] 尹德兴,西兰花栽培技术,农村实用技术与信息,2006,4.
[18] 张瑞明,青花菜主要病害发生与防治措施,上海蔬菜,2006,2.
[19] 赵前程,我国青花菜品种选育及其生产应用,当代蔬菜,2006,1.
[20] 李贵荣,翟玉兰,西兰花的栽培与保鲜技术,甘肃农业,2006,2.
[21] 高雁军,青花菜早春大棚有机栽培技术,现代农业,2006,3.
[22] 马兆义,金振兴,出口西兰花栽培技术,新农业,2006,2.
[23] 李光普, 胡云峰,微加工西兰花流通保鲜技术研究,中国农学通报,2005.
[24] 时厥祥, 陈涛,寒地西兰花栽培技术,中国农业综合开发,2005,12.
[25] 代聪和,李玉华,西兰花一代杂交制种产量低的原因与对策,蔬菜,2005,10.
[26] 叶利勇,朱剑桥,不同氮硼比对高山西兰花裂茎病的影响,浙江农业科学,2005,5.
[27] 周洪光,朱剑桥,高山夏秋季西兰花不同品种比较试验简报,温州农业科技,2005,3.
[28] 陈萍, 冯华卫,绿菜花杂交制种技术, 长江蔬菜,2005,10.
[29] 朱剑桥,叶利勇,春西兰花新品种——博爱一号品种特性及栽培技术简介,浙江亚热带作物通讯,2005,1.
[30] 王超,庞向举鄂西北延秋西兰花栽培技术,当代蔬菜,2005,8.
[31] 朱剑桥,周洪光,施硼对高山西兰花裂茎病的影响,温州农业科技,2005,2.
[32] 李丽红,毛进祥,夏秋季高山西兰花栽培试验,丽水农业科技,2005,2.
[33] 闫文海,孙发吉,凉州区西兰花栽培技术,甘肃农业科技,2005,5.
[34] 孔令娟,张志连,西兰花秋播试验初报,长江蔬菜-2005,5.
[35] 崔香仙,西兰花主要病害及其综合防治,特种经济动植物,2005,3.
[36] 梁炳坤,冬种西兰花田间管理关键技术,广东农村实用技术2005,2
[37] 金伟兴,陈林松,浙东沿海日本青花菜无公害高产优质栽培技术,蔬菜2005,3.
[38] 王国良,李国雷,西兰花花梗褐心病研究,植物保护2005,1.
[39] 陈海平,冯春梅,西兰花球茎褐变危害及病原鉴定初报,浙江农业科学2005,1.
[40] 刘飞,陈春秋,春西兰花穴盘规模化栽培技术,北方园艺2005,1.
[41] 万银红,赵明宽,利用高海拨夏季冷凉气候资源发展蔬菜生产——遵义县松林镇越夏西兰花种植实验报告,遵义科技,2004,4.
[42] 王崇明,马红娟,冬西兰花有机栽培技,上海蔬菜2007,1.
[43] 苏恩平,陈红辉,盛明峰,沈金芳,薛万新,青花菜新品种申绿二号的选育与栽培技术要点,上海蔬菜 2006,3
[44] 吴厚桂,青花菜新品种----马拉松,长江蔬菜 2007,5
[45] 陈澍棠,上海青花菜引种品种演变与发展趋势,上海农业科技, 1993,3
[46] 方智远,孙培田,刘玉梅等,青花菜自交不亲和系选育初报,中国蔬菜, 1987,1
[47] 陈澍棠,朱根娣1青花菜自交不亲和系S基因型分析及三交种配制初报[J ] ,上海农业学报, 1994,4
[48] 方智远,刘玉梅,孙培田等,青花菜杂种优势利用研究初报,中国蔬菜, 1990,6
[49] Dickon M H, G1117A , G11102A and G1106A cytosterile broccoli inbreds,Hort Science , 1975 , 10
[50] Yallow S A , Burnett L A , Wildeman R P ,etal. The transfer of'Polima'cytoplasmic male sterility from oilseed rape (Basic napus) to broccoli (B.oleracea) by protoplast fusion ,Plant cell Reports , 1990 , 9,4
[51] 任成伟,曹寿椿,萝卜细胞质芸薹属作物雄性不育材料的研究概况,中国蔬菜, 1992,2
[52] Liu Y M , Wang X W , Fang Z Y ,etal. A male sterile line with recessive gene in broccoli (Basic L. varitalicaPlanch) and its utilization . Cruciferae Newsletter , 1997 , 19
[53] 林碧英,魏文麟,青花菜细胞质雄性不育系的选育,福建农科院学报, 1997 , 11,1
[54] 朱玉英,姚文岳,张素琴等,Ogura细胞质甘蓝雄性不育系选育及其利用,上海农业学报, 1998 , 14,2
[55] Jourdan P S,Atrazine2resistant cauliflower obtained by somatic hybridization between Basic tolerance and ATR2B. napus .Theor Appl Genet , 1989(78)
[56] 魏宝琴, 魏毓棠,Ogura不育源结球白菜雄性不育系的选育,中国蔬菜, 1995(5)
[57] 黄帮全,李薇,居超民等,Ogura雄性不育细胞质导入紫菜薹及杂种优势利用初报,种子, 1999,3,57.
[58] 方智远, 孙培田, 刘玉梅,甘蓝胞质雄性不育系的选育简报,中国蔬菜, 1984,4
[59] 黄科等, CYP86MF反义基因转化获得青花菜雄性不育植株,中国农业科学,2005,1.
[60] 沈革志, 王新其, 朱玉英等,TA29-barnase 基因转化甘蓝产生雄性不育植株,植物生理学报, 2001, 27(1)
[61] Shen G Z, Wang X Q, Zhu Y Y, Yang H J, Male sterile transgenic cabbage plants with TA29-barnase Gene. Acta Phytophysiologica Sinica, 2001, 27(1) (in Chinese)
[62] 方智远, 孙培田, 刘玉梅, 杨丽梅, 侯安福. 青花菜杂种优势利用研究初报.,中国蔬菜, 1990 ,6 2-5.
[63] Fang Z Y , Sun P T, Liu Y M, Yang L M, Hou A F, Research of heterosis on Brassica oleracea. Chinese Vegetable,1990(6)(in Chinese)
[64] 李 贤, 姚泉洪, 庄 静, 陈海英, 范惠琴, 彭日荷, 熊爱生. ACC解氨酶基因转入青花菜的研究. 上海农业学报, 2001, 17,3
[65] Li X, Yao Q H, Zhuang J, Chen H Y, Fan H Q, Peng R H, Xiong A S. Research of ACC deaminase gene transferred into broccoli. Acta Agriculturae Shanghai, 2001,17(3)(in Chinese).
[66] Ye W Z, Cao J S,Xiang X, Zeng G W. Molecular cloning and characterization of the genic male sterility related gene CYP86MF in Chinese cabbage(Brassica campestris L. ssp. chinensis Makino var. communis Tsen et Lee). Journal of Horticultural Science and Biotechnology, 2003, 78(3)
[67] Li S X, Chen Y Y. The vascular tissue and the tracheary element in the calllus on hypocotyl of cabbage in vitro. Acta Botanica Boreali-Occidentalia Sinica. 1994,14(1)(in Chinese)
[68] Li S X Chen Y Y. Histological studies on the morphogenesis of the hypocotyl of cabbage in tissue cultures. Acta Botanica Boreali-Occidentalia Sinica, 1993,13(4).(in Chinese).
[69] 程振东, 卫志明, 许智宏, 根癌农杆菌对甘蓝型油菜的转化及转基因植的再生, 植物学报, 1994, 36(9)
[70] Cheng Z D, Wei Z M, Xu Z H. Transformation of Brassica napus using Agrobacterirm tumefaciens and regeneration of transgenic plants. Acta Botanica Sinica, 1994,.36(9)(in Chinese).
[71] 曹家树,叶纨芝,张 明等,白菜雄性不育两用系花蕾的mRNA差别显示及其cDNA差异片段分析,浙江大学学报(农业与生命科学版),2001,27(6)
[72] Cao J S, Ye W Z, Zhang M, Zeng G W. Differential display of flower bud mRNA of genic male sterility(GMS) AB line in Chinese cabbage-pak-choi and analysis of differential cDNA fragments. Journal of Zhejiang University(Agricultural & Life Science), 2001, 27(6)(in Chinese).
[73] Chakrabarty R, Viswakarma N, Bhar S R, Kirti P B. Agrobacterium-mediated transformation of cauliflower: optimization of protocol and development of Bt-transgenic cauliflower. Journal of Biosciences., 2002, 27(5)
[74] 曹家树,曹寿椿,易清明, 白菜及其相邻类群基因组DNA和RAPD分析, 园艺学报,1995,22,
[75] Zhang F C, Kang S Z, Li Z J. Effect of nitrogen forms on nitrate accumulation and nutrient absoption in cabbage. Acta Horticulturae Sinica, 2003,30(1)(in Chinese).
[76] Gehrig H H, Winter K, Cushman J, Borland A, Taybi T. An improved RNA isolation method for succulent plant species rich in polyphenols and polysaccharides. Plant Molecular Biology Reporter, 2000, 18
[77] Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, 1989
[78] 秦耀国,雷建军,曹必好,青花菜遗传育种与生物技术应JTI研究进展,北方园艺2004,2
[79] Thomas C E,Jourdain E L.EvMuaion of broccoli and cauliflower germplasm for resistance to race 2 of Peronospora parasifica.Hort Science,1990,11
[80] DicksonM H,PetzoldtR,Plant ageandisolatesouse afect expression of downy mildew resistance in broccoli,Hort Science, 1993,7
[81] CanadayCH,WyattJ E,Mu~ns JA.Resistancein broccolitobac.terial soft rot caused by Pseudomonas maminalis and fluorescent Pseu.domonas species.Plant Disease.1991,7
[82] 刘玉梅,孙培田,方智远等, 青花菜抗源材料的筛选和利用,中国蔬菜,1996,6
[83] 严继勇,青花菜主要性状在自交后代中的遗传稳定性,中国蔬菜,1999,6
[84] Baggett J R,Kean D,Kasimor K.Inheritance of internode length and its relation to head exsertion and head size in broocoli.Jounal of the American Society for Horticultural Science.1995, 2:292～296.
[85] Khattra A S,Gurmail Singh,ThakurJ C.eta1.Genotypic and pheno.typic correlations and path analysis studies in sprouting broccoli (Brassica oleracea vat.italica L).Journal of Research,Puniiab Agricultural University.2001
[86] Pritam—Kalia,Shakuntla,Kalia P.Genetic variability for horticu1.tural characters in green sprouting broccoli.Indian Journal of Horticu1ture.2002,1
[87] Jensen B D,Vaerbak S,Munk L.Characterization and inheritance of partial resistance to downy mildew,Peronospora pa rasifica,in breeding material of broccoli,Brassica oleracea convar.botrytis var.italica .Plant Breeding.1999.6
[88] Wang M,Farnham M W.Thomas C E.Inheritance of true leaf stage downymildew resistancein broccoli.J.Am.Soc.Hortic.Sci.2001,6
[89] 严继勇,李式军,徐鹤林.青花菜抗冻性的遗传分析.南京农业大学学报,1995.18(1).
[90] 李长缨,简元才,杜广岑等,青花菜耐贮性鉴定方法和标准,华北农学报,1999,(14),4.
[91] 方智远,青花菜杂种优势利用研究初报 ,中国蔬菜,1990 ,06
[92] 张克平,青花菜一代杂种绿宝的选育,中国蔬菜,1997 ,02 ,36
[93] 林碧英,青花菜细胞质雄性不育系的选育 ,福建省农科院学报,1997,(12),01
[94] 侯喜林,十字花科作物几种主要细胞质雄性不育系及其利用 , 长江蔬菜,1999 ,(5),6
[95] 张克平, 林荔仙,青花菜雄性不育系选育研究初报,亚热带植物科学,2002,3
[96] 赵志国,支荣英,青花菜无公害栽培技术,西北园艺,2007 ,1
[97] 汪恩国 , 陈克松 , 李达林等,青花菜黑腐病空间分布格局测定及其应用,中国植保导刊 ,2004,2
[98] WANG Chao , ZHANG Gui-ling , WU Shi-chang,Identification of Resistance to Blackrot in Broccoli,东北农业大学学报(英文版),2002,2
[2] 曹东贺,圆葱与西兰花复种技术,新农业,2006,11.
[3] 牟水元,温室西兰花秋冬栽培技术,农技服务,2006,10.
[4] 李建良, 王慧芬,西兰花主要病虫害及其无公害防治技术,北京农业,2006,9.
[5] 姚学竹,景泰县地膜西兰花无公害栽培技术,甘肃农业科技,2006,9.
[6] 王国升,黄曼华,夏秋反季节西兰花优质高产无公害栽培技术,耕作与栽培,2006,4.
[7] 周玉玲,大棚春青花菜-夏甘蓝-秋番茄一年三种三收模式,西北园艺,2006,5.
[8] 刘威,张云川,不同漂烫温度对西兰花品质指标的影响,农产品加工学刊,2007,1.
[9] 赵志国,支荣英,青花菜无公害栽培技术,西北园艺,蔬菜,2007,1.
[10] 柴守霞,“防癌新秀”西兰花,家庭护士,2007,1.
[11] 陈秋松,西兰花新品--福至二号,农家之友,2006,11.
[12] 谢以泽, 黄新灿,甘蓝·西兰花主要病虫害无害化防治技术的大田控制效果,安徽农业科学, 2006,21.
[13] 高旭春,邹桂花,青花菜四季高产栽培技术,当代蔬菜-2006年8期
[14] 刘振铃,夏庆伟,西兰花一代杂交制种产量低的原因与科学的采种方法,上海蔬菜,2006,3.
[15] 许广祥,马东西兰花生理病害防控措施,新农业,2006,4.
[16] 陈海平,董荷玲,台州市西兰花标准化栽培技术,长江蔬菜,2006,5.
[17] 尹德兴,西兰花栽培技术,农村实用技术与信息,2006,4.
[18] 张瑞明,青花菜主要病害发生与防治措施,上海蔬菜,2006,2.
[19] 赵前程,我国青花菜品种选育及其生产应用,当代蔬菜,2006,1.
[20] 李贵荣,翟玉兰,西兰花的栽培与保鲜技术,甘肃农业,2006,2.
[21] 高雁军,青花菜早春大棚有机栽培技术,现代农业,2006,3.
[22] 马兆义,金振兴,出口西兰花栽培技术,新农业,2006,2.
[23] 李光普, 胡云峰,微加工西兰花流通保鲜技术研究,中国农学通报,2005.
[24] 时厥祥, 陈涛,寒地西兰花栽培技术,中国农业综合开发,2005,12.
[25] 代聪和,李玉华,西兰花一代杂交制种产量低的原因与对策,蔬菜,2005,10.
[26] 叶利勇,朱剑桥,不同氮硼比对高山西兰花裂茎病的影响,浙江农业科学,2005,5.
[27] 周洪光,朱剑桥,高山夏秋季西兰花不同品种比较试验简报,温州农业科技,2005,3.
[28] 陈萍, 冯华卫,绿菜花杂交制种技术, 长江蔬菜,2005,10.
[29] 朱剑桥,叶利勇,春西兰花新品种——博爱一号品种特性及栽培技术简介,浙江亚热带作物通讯,2005,1.
[30] 王超,庞向举鄂西北延秋西兰花栽培技术,当代蔬菜,2005,8.
[31] 朱剑桥,周洪光,施硼对高山西兰花裂茎病的影响,温州农业科技,2005,2.
[32] 李丽红,毛进祥,夏秋季高山西兰花栽培试验,丽水农业科技,2005,2.
[33] 闫文海,孙发吉,凉州区西兰花栽培技术,甘肃农业科技,2005,5.
[34] 孔令娟,张志连,西兰花秋播试验初报,长江蔬菜-2005,5.
[35] 崔香仙,西兰花主要病害及其综合防治,特种经济动植物,2005,3.
[36] 梁炳坤,冬种西兰花田间管理关键技术,广东农村实用技术2005,2
[37] 金伟兴,陈林松,浙东沿海日本青花菜无公害高产优质栽培技术,蔬菜2005,3.
[38] 王国良,李国雷,西兰花花梗褐心病研究,植物保护2005,1.
[39] 陈海平,冯春梅,西兰花球茎褐变危害及病原鉴定初报,浙江农业科学2005,1.
[40] 刘飞,陈春秋,春西兰花穴盘规模化栽培技术,北方园艺2005,1.
[41] 万银红,赵明宽,利用高海拨夏季冷凉气候资源发展蔬菜生产——遵义县松林镇越夏西兰花种植实验报告,遵义科技,2004,4.
[42] 王崇明,马红娟,冬西兰花有机栽培技,上海蔬菜2007,1.
[43] 苏恩平,陈红辉,盛明峰,沈金芳,薛万新,青花菜新品种申绿二号的选育与栽培技术要点,上海蔬菜 2006,3
[44] 吴厚桂,青花菜新品种----马拉松,长江蔬菜 2007,5
[45] 陈澍棠,上海青花菜引种品种演变与发展趋势,上海农业科技, 1993,3
[46] 方智远,孙培田,刘玉梅等,青花菜自交不亲和系选育初报,中国蔬菜, 1987,1
[47] 陈澍棠,朱根娣1青花菜自交不亲和系S基因型分析及三交种配制初报[J ] ,上海农业学报, 1994,4
[48] 方智远,刘玉梅,孙培田等,青花菜杂种优势利用研究初报,中国蔬菜, 1990,6
[49] Dickon M H, G1117A , G11102A and G1106A cytosterile broccoli inbreds,Hort Science , 1975 , 10
[50] Yallow S A , Burnett L A , Wildeman R P ,etal. The transfer of'Polima'cytoplasmic male sterility from oilseed rape (Basic napus) to broccoli (B.oleracea) by protoplast fusion ,Plant cell Reports , 1990 , 9,4
[51] 任成伟,曹寿椿,萝卜细胞质芸薹属作物雄性不育材料的研究概况,中国蔬菜, 1992,2
[52] Liu Y M , Wang X W , Fang Z Y ,etal. A male sterile line with recessive gene in broccoli (Basic L. varitalicaPlanch) and its utilization . Cruciferae Newsletter , 1997 , 19
[53] 林碧英,魏文麟,青花菜细胞质雄性不育系的选育,福建农科院学报, 1997 , 11,1
[54] 朱玉英,姚文岳,张素琴等,Ogura细胞质甘蓝雄性不育系选育及其利用,上海农业学报, 1998 , 14,2
[55] Jourdan P S,Atrazine2resistant cauliflower obtained by somatic hybridization between Basic tolerance and ATR2B. napus .Theor Appl Genet , 1989(78)
[56] 魏宝琴, 魏毓棠,Ogura不育源结球白菜雄性不育系的选育,中国蔬菜, 1995(5)
[57] 黄帮全,李薇,居超民等,Ogura雄性不育细胞质导入紫菜薹及杂种优势利用初报,种子, 1999,3,57.
[58] 方智远, 孙培田, 刘玉梅,甘蓝胞质雄性不育系的选育简报,中国蔬菜, 1984,4
[59] 黄科等, CYP86MF反义基因转化获得青花菜雄性不育植株,中国农业科学,2005,1.
[60] 沈革志, 王新其, 朱玉英等,TA29-barnase 基因转化甘蓝产生雄性不育植株,植物生理学报, 2001, 27(1)
[61] Shen G Z, Wang X Q, Zhu Y Y, Yang H J, Male sterile transgenic cabbage plants with TA29-barnase Gene. Acta Phytophysiologica Sinica, 2001, 27(1) (in Chinese)
[62] 方智远, 孙培田, 刘玉梅, 杨丽梅, 侯安福. 青花菜杂种优势利用研究初报.,中国蔬菜, 1990 ,6 2-5.
[63] Fang Z Y , Sun P T, Liu Y M, Yang L M, Hou A F, Research of heterosis on Brassica oleracea. Chinese Vegetable,1990(6)(in Chinese)
[64] 李 贤, 姚泉洪, 庄 静, 陈海英, 范惠琴, 彭日荷, 熊爱生. ACC解氨酶基因转入青花菜的研究. 上海农业学报, 2001, 17,3
[65] Li X, Yao Q H, Zhuang J, Chen H Y, Fan H Q, Peng R H, Xiong A S. Research of ACC deaminase gene transferred into broccoli. Acta Agriculturae Shanghai, 2001,17(3)(in Chinese).
[66] Ye W Z, Cao J S,Xiang X, Zeng G W. Molecular cloning and characterization of the genic male sterility related gene CYP86MF in Chinese cabbage(Brassica campestris L. ssp. chinensis Makino var. communis Tsen et Lee). Journal of Horticultural Science and Biotechnology, 2003, 78(3)
[67] Li S X, Chen Y Y. The vascular tissue and the tracheary element in the calllus on hypocotyl of cabbage in vitro. Acta Botanica Boreali-Occidentalia Sinica. 1994,14(1)(in Chinese)
[68] Li S X Chen Y Y. Histological studies on the morphogenesis of the hypocotyl of cabbage in tissue cultures. Acta Botanica Boreali-Occidentalia Sinica, 1993,13(4).(in Chinese).
[69] 程振东, 卫志明, 许智宏, 根癌农杆菌对甘蓝型油菜的转化及转基因植的再生, 植物学报, 1994, 36(9)
[70] Cheng Z D, Wei Z M, Xu Z H. Transformation of Brassica napus using Agrobacterirm tumefaciens and regeneration of transgenic plants. Acta Botanica Sinica, 1994,.36(9)(in Chinese).
[71] 曹家树,叶纨芝,张 明等,白菜雄性不育两用系花蕾的mRNA差别显示及其cDNA差异片段分析,浙江大学学报(农业与生命科学版),2001,27(6)
[72] Cao J S, Ye W Z, Zhang M, Zeng G W. Differential display of flower bud mRNA of genic male sterility(GMS) AB line in Chinese cabbage-pak-choi and analysis of differential cDNA fragments. Journal of Zhejiang University(Agricultural & Life Science), 2001, 27(6)(in Chinese).
[73] Chakrabarty R, Viswakarma N, Bhar S R, Kirti P B. Agrobacterium-mediated transformation of cauliflower: optimization of protocol and development of Bt-transgenic cauliflower. Journal of Biosciences., 2002, 27(5)
[74] 曹家树,曹寿椿,易清明, 白菜及其相邻类群基因组DNA和RAPD分析, 园艺学报,1995,22,
[75] Zhang F C, Kang S Z, Li Z J. Effect of nitrogen forms on nitrate accumulation and nutrient absoption in cabbage. Acta Horticulturae Sinica, 2003,30(1)(in Chinese).
[76] Gehrig H H, Winter K, Cushman J, Borland A, Taybi T. An improved RNA isolation method for succulent plant species rich in polyphenols and polysaccharides. Plant Molecular Biology Reporter, 2000, 18
[77] Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, 1989
[78] 秦耀国,雷建军,曹必好,青花菜遗传育种与生物技术应JTI研究进展,北方园艺2004,2
[79] Thomas C E,Jourdain E L.EvMuaion of broccoli and cauliflower germplasm for resistance to race 2 of Peronospora parasifica.Hort Science,1990,11
[80] DicksonM H,PetzoldtR,Plant ageandisolatesouse afect expression of downy mildew resistance in broccoli,Hort Science, 1993,7
[81] CanadayCH,WyattJ E,Mu~ns JA.Resistancein broccolitobac.terial soft rot caused by Pseudomonas maminalis and fluorescent Pseu.domonas species.Plant Disease.1991,7
[82] 刘玉梅,孙培田,方智远等, 青花菜抗源材料的筛选和利用,中国蔬菜,1996,6
[83] 严继勇,青花菜主要性状在自交后代中的遗传稳定性,中国蔬菜,1999,6
[84] Baggett J R,Kean D,Kasimor K.Inheritance of internode length and its relation to head exsertion and head size in broocoli.Jounal of the American Society for Horticultural Science.1995, 2:292～296.
[85] Khattra A S,Gurmail Singh,ThakurJ C.eta1.Genotypic and pheno.typic correlations and path analysis studies in sprouting broccoli (Brassica oleracea vat.italica L).Journal of Research,Puniiab Agricultural University.2001
[86] Pritam—Kalia,Shakuntla,Kalia P.Genetic variability for horticu1.tural characters in green sprouting broccoli.Indian Journal of Horticu1ture.2002,1
[87] Jensen B D,Vaerbak S,Munk L.Characterization and inheritance of partial resistance to downy mildew,Peronospora pa rasifica,in breeding material of broccoli,Brassica oleracea convar.botrytis var.italica .Plant Breeding.1999.6
[88] Wang M,Farnham M W.Thomas C E.Inheritance of true leaf stage downymildew resistancein broccoli.J.Am.Soc.Hortic.Sci.2001,6
[89] 严继勇,李式军,徐鹤林.青花菜抗冻性的遗传分析.南京农业大学学报,1995.18(1).
[90] 李长缨,简元才,杜广岑等,青花菜耐贮性鉴定方法和标准,华北农学报,1999,(14),4.
[91] 方智远,青花菜杂种优势利用研究初报 ,中国蔬菜,1990 ,06
[92] 张克平,青花菜一代杂种绿宝的选育,中国蔬菜,1997 ,02 ,36
[93] 林碧英,青花菜细胞质雄性不育系的选育 ,福建省农科院学报,1997,(12),01
[94] 侯喜林,十字花科作物几种主要细胞质雄性不育系及其利用 , 长江蔬菜,1999 ,(5),6
[95] 张克平, 林荔仙,青花菜雄性不育系选育研究初报,亚热带植物科学,2002,3
[96] 赵志国,支荣英,青花菜无公害栽培技术,西北园艺,2007 ,1
[97] 汪恩国 , 陈克松 , 李达林等,青花菜黑腐病空间分布格局测定及其应用,中国植保导刊 ,2004,2
[98] WANG Chao , ZHANG Gui-ling , WU Shi-chang,Identification of Resistance to Blackrot in Broccoli,东北农业大学学报(英文版),2002,2