多孔型丝素蛋白/羟基磷灰石修复兔股骨包含性骨缺损
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摘要
目的:通过组织工程方法研究丝素蛋白/羟基磷灰石复合材料的细胞相容性及其修复包含性骨缺损的作用,探讨其临床应用的可能性。
方法:同种异体脂肪干细胞(Adipose-derived stromal cells,ADSCs)与丝素蛋白/羟基磷灰石共同培养构建组织工程骨。取新西兰大白兔36只,随机分为A、B、C 3组,每组12只,在兔股骨远端制造一4.5mm直径、10mm深度松质骨缺损。A组为实验组,植入复合ADSCs的丝素蛋白/羟基磷灰石;B组为实验对照组,单纯植入丝素蛋白/羟基磷灰石;C组为空白对照组,不作任何植入。
结果:ADSCs能在SF/HA材料上良好的黏附、增殖和生长。12周时X线、组织学检查显示A组完全修复了骨缺损区,B组部分修复了骨缺损区,A组的新生骨小梁多于B组(P <0.05)。C组未见骨修复。
结论:SF/HA具有良好的细胞相容性,并能成功修复兔股骨包含性骨缺损,有望成为骨组织工程理想的细胞外基质和骨移植替代材料。
Objective: To investigate the cellular compatibility of silk fibroin/hydroxyapatite (SF/HA) and its efficacy in repairing contained bone defects, and to explore the possibility of its clinical application.
Methods: SF/HA was co-cultured with Adipose-derived stromal cells(ADSCs)to repair bone defects.36 rabbits were randomly divided into 3 groups. Each group had 12 rabbits. The bone defects(4.5mm×4.5mm×10mm) in the condylus lateralis femoris of rabbits were implanted with ADSCs-SF/HA complex(group A),with simple SF/HA(group B) or left untreated(group C).
Result: Normal adherence, proliferation and growth of ADSCs on the SF/HA could be seen. The examination of X-ray and histology showed that the bone defects were repaired entirely in group A and partly in group B. The quantity of the newly formed bone in group A was more than that in group B (P <0.05). Repair showed no effect in group C.
Conclusion: SF/HA has good cellular compatibility, and can successfully repair contained bone defects of a rabbit femur. It can be used as the ideal alternative biomaterial for bone tissue engineering.
方法:同种异体脂肪干细胞(Adipose-derived stromal cells,ADSCs)与丝素蛋白/羟基磷灰石共同培养构建组织工程骨。取新西兰大白兔36只,随机分为A、B、C 3组,每组12只,在兔股骨远端制造一4.5mm直径、10mm深度松质骨缺损。A组为实验组,植入复合ADSCs的丝素蛋白/羟基磷灰石;B组为实验对照组,单纯植入丝素蛋白/羟基磷灰石;C组为空白对照组,不作任何植入。
结果:ADSCs能在SF/HA材料上良好的黏附、增殖和生长。12周时X线、组织学检查显示A组完全修复了骨缺损区,B组部分修复了骨缺损区,A组的新生骨小梁多于B组(P <0.05)。C组未见骨修复。
结论:SF/HA具有良好的细胞相容性,并能成功修复兔股骨包含性骨缺损,有望成为骨组织工程理想的细胞外基质和骨移植替代材料。
Objective: To investigate the cellular compatibility of silk fibroin/hydroxyapatite (SF/HA) and its efficacy in repairing contained bone defects, and to explore the possibility of its clinical application.
Methods: SF/HA was co-cultured with Adipose-derived stromal cells(ADSCs)to repair bone defects.36 rabbits were randomly divided into 3 groups. Each group had 12 rabbits. The bone defects(4.5mm×4.5mm×10mm) in the condylus lateralis femoris of rabbits were implanted with ADSCs-SF/HA complex(group A),with simple SF/HA(group B) or left untreated(group C).
Result: Normal adherence, proliferation and growth of ADSCs on the SF/HA could be seen. The examination of X-ray and histology showed that the bone defects were repaired entirely in group A and partly in group B. The quantity of the newly formed bone in group A was more than that in group B (P <0.05). Repair showed no effect in group C.
Conclusion: SF/HA has good cellular compatibility, and can successfully repair contained bone defects of a rabbit femur. It can be used as the ideal alternative biomaterial for bone tissue engineering.
引文
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