脱钙牙基质的物理表征及骨诱导活性研究
|
摘要
目的:
研究骨诱导材料脱钙牙基质的形貌、结构和骨诱导活性,为脱钙牙基质临床应用提供实验和理论依据。
方法:
1.以标准羟基磷灰石为对照,采用场发射扫描电镜,付立叶红外光谱仪,X一射线衍射光谱仪对脱钙牙基质的大小、形貌、组成及晶相结构进行观测和分析。
2.以标准羟基磷灰石粉末为对照组,将脱钙牙基质与MC-3T3成骨细胞在体外复合培养,采用MTT法和ALP检测法检测脱钙牙基质对成骨细胞的增殖及碱性磷酸酶活性的影响。
结果:
1.脱钙牙基质颗粒呈圆棒状,大小均匀,成分主要为羟基磷灰石,并含有CaPO_3(OH) 2H_2O和含水的Ca_3(PO_4)_2等磷酸盐成分。
2.脱钙牙基质能够促进成骨细胞的增殖及提高碱性磷酸酶的活性。
结论:
脱钙牙基质具有羟基磷灰石的主要物理特征,具有良好的生物相容性和骨诱导活性。
Objective:
Morphology,structure and bone induction activity of demineralized dental matrix were studied to provide experimental and theoretical base for the clinical application of the demineralized dentin matrix(DDM).
Methods:
1.With standard hydroxyapatite as control,the morphology, structure and bone induction activity of the demineralized dental matrix were characterized by scanning electron microscopy,Fourier transform infrared spectroscopy and X-ray grating spectrum.
2.MC-3T3 osteoblasts were cultured with demineralized dental matrix in vitro with standard hydroxyapatite as control.The influence of demineralized dental matrix on proliferation and alkaline phosphatase activity in osteoblasts were evaluated by MTT and ALP methods.
Results:
1.Demineralized dental matrix consists mainly of hydroxyapatite, and contains other phosphates such as CaPO_3(OH)·2H_2O and Ca_3(PO_4) _2·XH_2O.Its granules were rod-shape and same in size.
2.Demineralized dental matrix can promote proliferation and alkaline phosphatase activity of osteoblasts.
Conclusion:
Demineralized dental matrix has the chiefly characterization of hydroxyapatite,it is an ideal filling material with great osteoconduction and biocompatibility.
研究骨诱导材料脱钙牙基质的形貌、结构和骨诱导活性,为脱钙牙基质临床应用提供实验和理论依据。
方法:
1.以标准羟基磷灰石为对照,采用场发射扫描电镜,付立叶红外光谱仪,X一射线衍射光谱仪对脱钙牙基质的大小、形貌、组成及晶相结构进行观测和分析。
2.以标准羟基磷灰石粉末为对照组,将脱钙牙基质与MC-3T3成骨细胞在体外复合培养,采用MTT法和ALP检测法检测脱钙牙基质对成骨细胞的增殖及碱性磷酸酶活性的影响。
结果:
1.脱钙牙基质颗粒呈圆棒状,大小均匀,成分主要为羟基磷灰石,并含有CaPO_3(OH) 2H_2O和含水的Ca_3(PO_4)_2等磷酸盐成分。
2.脱钙牙基质能够促进成骨细胞的增殖及提高碱性磷酸酶的活性。
结论:
脱钙牙基质具有羟基磷灰石的主要物理特征,具有良好的生物相容性和骨诱导活性。
Objective:
Morphology,structure and bone induction activity of demineralized dental matrix were studied to provide experimental and theoretical base for the clinical application of the demineralized dentin matrix(DDM).
Methods:
1.With standard hydroxyapatite as control,the morphology, structure and bone induction activity of the demineralized dental matrix were characterized by scanning electron microscopy,Fourier transform infrared spectroscopy and X-ray grating spectrum.
2.MC-3T3 osteoblasts were cultured with demineralized dental matrix in vitro with standard hydroxyapatite as control.The influence of demineralized dental matrix on proliferation and alkaline phosphatase activity in osteoblasts were evaluated by MTT and ALP methods.
Results:
1.Demineralized dental matrix consists mainly of hydroxyapatite, and contains other phosphates such as CaPO_3(OH)·2H_2O and Ca_3(PO_4) _2·XH_2O.Its granules were rod-shape and same in size.
2.Demineralized dental matrix can promote proliferation and alkaline phosphatase activity of osteoblasts.
Conclusion:
Demineralized dental matrix has the chiefly characterization of hydroxyapatite,it is an ideal filling material with great osteoconduction and biocompatibility.
引文
[1]张晓,颜晓慧．骨组织工程细胞外基质材料[J]．现代康复,2001,5(6下)：18-19．
[2]Cochran DL, Schenk R, Buser D, et al. Corrosion behaviour of commercially pure titanium shot blasted with different materials and sizes of shot particles for dental implant applications[J]. Periodontol, 1999, 70: 139-50.
[3]Urist MR, Delange RJ, Finerman GAM. Bone cell differentiation and growth factors induced activity of chondroosteogenic DNA [J]. Science. 1983, 220(4598):680-6
[4]Urist MR, Selang RH. Methods of preparation and bioassay of bone morphogenetic protein and polypeptide fragments [M]. Barnes D, Sirbaska DA, eds. Methods in Enzymology. vol. 146. New York:Academy Press, 1987: 294.
[5]Bergsma JE, Rozema FR , Bos RR, et al. Biocompatibility study of as polymetized poly(L-lactide) in rats using a cage implant system[J]. Biomed Mater Res ,1995 ,29 (2) :173 - 9
[6]Wheater PH, Burkitt HG, Daniels VG. Functional histology[M]. London:Churchill livingstone, 1982: 128
[7] Urist MR. Experimental delivery systems for bone morphogenetic protein. In:Wise DL, eds. Hand book of Biomaterials and Applications, Section Orthopaedic Biomaterials Applications[J]. Boston , Mass : Marcel Dekker ,1995,3:1093-133.
[8]Urist MR, Lietze A, Mizutani H, et al. A bovine low molecular weight bone morphogenetic protein (BMP) fraction[J].Clin Orthop, 1982,162:219
[9]Kinoshita A, Oda S, Takahashi K, et al. Periodontal regeneration by application of recombinant human bone morphogenetic protein-2 to horizontal circumferential defects created by experimental periodontitis in beagle dogs[J]. Periodontol, 1997, 68(2):103-9
[10]Sigurdsson TJ, Lee M, Kukota R, et al. Periodontal repair in dogs: recombinant human bone morphogenetic protein significantly enhances periodontal regeneration[J].Periodontol,1995,66(2):131-8
[11]岑远坤,李文,廖运茂,等.脱钙牙本质基质及其复合物整复节段性骨缺损的组织学观察[J].华西口腔医学杂志,1998;16(3):263-265
[12]杨连甲,金岩,胡蕴玉主编.口腔和骨科的生物活性材料[M].西安:陕西科学技术出版社,1993:141-231.
[13]Merry J.C,Gibsm I.R,Best S.M,et al.Synthesis and characterization of carbonate hydroxyapatite[J].Mater Sci Mater Med,1998,12:779-83.
[14]Azic S,Popovic J K,Zec S,et al.Properties of hydroxyapatitellized from high temperature alkaline solutions[J].Cryst Growth.1996,165:124-8.
[15]Shirkhanzadeh M.Bioactive calcium phosphate coatingsprepared by electrodeposition[J].Mater Sci Lett,1991,10:1415-17.
[16]李世普.生物医用材料导论[M].武汉:武汉工业大学出版社.2000(8):88-123.
[17]Suchanek W,Y oshimura M.Processing and properties of hydroxyapatite 2based biomaterials for use as hard tissue replacement implants[J].Mater Res,1998,13:94-117.
[18]Kumar R,Prakash KH,Cheang P,et al.Temperature driven morphological changes of chemically precipitated hydroxyapatite nanoparticles[J].Langmuir.2004,20(13):5196-200.
[19]Liu DM,Yang Q,Troczynski T,et al.Structural evolution of sol-gel-derived hydroxyapatite[J].Biomaterials 2002,23(7):1679-87.
[20]Adkisson H.D,Strauss-Schoenberger J,Gillis M,Rapid quantitative bioassay of osteoinduction[J].Orthop Res.2000,18(3):503-11.
[21]Aaron RK,Ciombor DM.Acceleration of experimental endochondral ossification by biophysical stimulation of the progenitor cell pool[J].Orthop Res,1996,14(4):582.
[22]程玉华,赵卜军,刘海俊,等.胶原复合羟基磷灰石修复下颌骨缺损的组 织学观察[J].中国修复重建外科杂志,1998,12(2):74
[23]刘斌.种植体固定增高牙槽嵴的动物实验研究.[P].华西医科大学硕士学位论文,2000;
[24]Mosman T.Rapid colorimetric assay for cellular growth and survival:Application to proliferation and cytotoxicity assays[J].Immunol Methods,1983,65(1-2):55-63.
[25]Sjogren G,Sletten G,Dahl J.E,Cytotoxicity of dental alloys,metals,and ceramics assessed by Millipore filter,agar overlay,and MTT tests[J].Journal of Prosthetic Dentistry,2000,84(2):229-36.
[26]Morrison C,Macnair R,Macdonald C,et al.In Vitro Biocompatibility Testing of Polymers for Orthopedic Implants Using Cultured Fibroblasts and Osteoblasts[J].Biomaterials,1995,16(13):987-92.
[27]Timmer M.D,Shin H,Horch R.A,et al.In vitro cytotoxicity of injectable and biodegradable poly(propylene fumarate)-basednetworks:Unreacted macromers,cross-linked networks,and degradation products[J].Biomacromolecules,2003,4(4):1026-33.
[28]Thonemann B,Schmalz G,Hiller K.A,et al.Responses of L929 mouse fibroblasts,primary and immortalized bovine dental papilla-derived cell lines to dental resin components[J].Dental Materials,2002,18(4):318-23.
[29]Herbertson A,Aubin JE.Cell sorting entices osteogenic populations in rat bonemarrow stromal cell cultures[J].Bone,1997,21(6):491
[30]Aubin J.E,Advances in the osteoblast lineage[J].Biochem Cell Biol,1998,76(6):899-910.
[31]Takahashi Y,Yamamoto M,Tabata Y.Osteogenic differentiation of mesenchymal step cells in biodegradable sponges composed of gelatin and beta-tricalcium phosphate[J].Biomaterials,2005,26(17):3587-96.
[1]Urist MR,hietze A,Mizutani H,et al.A bovine low molecular weight bone morphogenetic protein(BMP) fraction[J].Clin Orthop.1982,162:219.
[2]Aaron RE,Ciombor DM.Acceleration of experimental endochondral ossi-Fication by biophysical stimulation of the progenitor cell pool[J].Orthop Res,1996,14(4):582.
[3]程玉华,赵卜军,刘海俊,等.胶原复合羟基磷灰石修复下颌骨缺损的组织学观察[J].中国修复重建外科杂志,1998,12(2):74
[4]李起鸿.我国修复长骨大段骨缺损的进展[J].中华骨科杂志,1997,17(1):13
[5]岑远坤,李文,廖运茂,等.脱钙牙本质基质及其复合物整复节段性骨缺损的组织学观察[J].华西口腔医学杂志,1998,16(3):263
[6]杨连甲,金岩,胡蕴玉主编.口腔和骨科的生物活性材料[M].西安:陕西科学技术出版社,1993:141-231.
[7]Zehnder M,Waltimo N,Sener B,et al.Dentin enhances the effectiveness of bioactive glass S53P4 against a strain of Enterococcus faecalis[J].Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics,2006,101(4):530-5.
[8]Zhang Y,Agee KNor J,et al.Effects of acid-etching on the tensile properties of demineralized dentin matrix[J].Dental Materials,1998,14(3):222-228.
[9]C.Begue-Kirn,P.H.Krebsbach,J.D.Bartlett,et al.Dentin sialoprotein,dentin phosphoprotein,enamelysin and ameloblastin:tooth-specific molecules that are distinctively expressed during murine dental dierentiation [J].Oral Sci.1998,106:963-70.
[10]M.E.Nimni,R.D.Harkness,Molecular structures and functions of collagens[M].Nimni,M.E.(Ed.),Collagen:vol.Ⅰ,Biochemistry.CRC Press,Boca Raton,FL,1988,pp.1-79.
[11]D.A.D.Parry,A.S.Craig,Collagen fibrils during development[M].In:Nimni,M.E.(Ed.),Collagen.CRC Press,Boca Raton,FL,1988,pp.1-20.
[12]B.Brodsky,S.Tanaka,E.F.Eikenberry,et al.X-ray diffraction as a tool for studying collagen structure[M]. In: Nimni, M. E. (Ed.), Collagen: vol. I, Biochemistry. CRC Press, Boca Raton, FL, p. 5v.
[13]R. Garberoglio, M. Brannstorm, Scanning electron microscopic investigation of human dentinal tubules[J]. Arch.Oral Biol. 1976,21:355-62.
[14]D. H. Pashley, Clinical correlations of dentin structure and function [J]. Prosthet. Dent. 1991, 66:777-81.
[15] J. Perdigao, P. Lambrechts, B. Van Meerbeek, et al. Morphological field emission - SEM study of the elect of six phosphoric acid etching agents on human dentin[J]. Am. J. Dent. Mater. 1996, 12:262-71.
[16] J. K. Avery, Oral Development and Histology B.C. Dekker Toronto[M], Philadelphia. 1988.
[17]C. P. Lin, W. H. Douglas, S. L. Erlandsen, Scanning electron microscopy of type I collagen at the dentin - enamel junction of human teeth[J]. Histochem Cytochem. 1993,41:381-88.
[18]A. L. Arsenault, A comparative electron microscopic study of apatite crystals in collagen fibrils of rat bone, dentin and calcified turkey leg tendons[J]. Bone Miner. 1989,6:165-77.
[19] J. Perdigao, J.Y.Thompson, M. Toledano, et al. Anultra-morphological characterization of collagen-depleted etched dentin [J]. Am J Dent, 1999. 12:250-55.
[20]K. D. Moses, W. T. Butler, C. Qin, Immunohistochemical study of small integrin-binding ligand, N-linked glycoproteins in reactionary dentin of rat molars at dierent ages[J]. Oral. Sci. 2006,114:216-22.
[21] LF. Massa, A. Ramachandran, A.George, et al. Developmental appearance of dentin matrix protein 1 during the early dentinogenesis in rat molars as identified by high-resolution immunocytochemistry, Histochem[J]. Cell Bio2005,124:197-205.
[22] R. N. D Souza, A. Cavender, G, Sunavala , et al. Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo[J]. Bone Miner Res. 1997,12:2040-49.
[23]N. Kamiya, M. Takagi, Differential, Expression of dentin matrix protein 1, type I collagen and osteocalcin genes in rat developing mandibular bone[J].Histochem 2001,33:545-52.
[24] J.Q.Feng, H.Huang, Y. Lu, et al. The Dentinmatrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development[J]. Dent Res. 2003, 82:776-80.
[25]P. H. Tartaix, M. Doulaverakis, A. George, et al. In vitro effects of dentinmatrix protein-1 on hydroxyapatite formation provide insights into in vivo functions[J].Biol Chem. 2004,179:18115-20.
[26]G. He, T. Dahl, A. Veis, et al. Dentin matrix protein 1 initiates hydroxyapatite formation in vitro [J]. Connect Tissue Res. 2003, 44:240-45.
[27] J. L. Pang, B. L. Wu, W. X. He, et al. Effect of antisense oligonucleotide against mouse dentine matrix protein 1 on mineralization ability and calcium ions metabolism in odontoblast-like cell line MDPC-23[J]. Endod. 2006,39:527-37.
[28]G. He, S. Gajjeraman, D. Schultz, et al. Spatially and temporally controlled biomineral-ization is facilitated by interaction between self-assembled dentin matrix protein 1 and calcium phosphate nuclei in solution[J]. Biochemistry , 2005, 44:16140 - 48.
[29]A. Almushayt, K. Narayanan, A. E. Zaki, et al. Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odonto-blasts, Gene[J]. Ther. 2006, 13:611-20.
[30]K. Narayanan, R. Srinivas, A. Ramachandran, et al. Differentiation of embryonic mesenchymal cells to odonto-blast-like cells by over-expression of dentin matrix protein 1, Proc. Natl[J]. Acad. Sci. USA 2001, 98:4516-21.
[31]Stefan Habelitz, Mehdi Balooch, Sally J. Marshall, et al. In situ atomic force microscopy of partially demineralized human dentin collagen fibrils[J]. Journal of Structural Biology, 2002,138:227-36.
[32] J. H. Kinney, D. L.Haupt,M. Balooch, et al. The threshold effects of Nd and Ho: YAG laser-induced surface modification on demineralization of dentin surfaces[J]. Dent Res, 1996, 75:1388-95.
[33]G. W.Marshall, S.J.Marshall, J. H. Kinney, et al. The dentin substrate: structure and properties related to bonding[J]. Dent. 1997, 25:441-58.
[34] F. El Feninat, T.H.Ellis, E. Sacher, et al. A tapping mode AFM study of collapse and denaturation in dentinal collagen[J]. Dent. Mater. 2001, 17:284-8.
[35]E. Baer, J. J. Cassidy, A. Hiltner, Hierachical structure of collagen and its relationship to the physical properties of tendon[M]. In: Nimni, M. E. (Ed.), Collagen: vol. II, Biochemistry and Biomechanics. CRC Press, Boca Raton, FL, p. 5v. 1988.
[36]W. J. Landis, J.Moradian-Oldak, S.Weiner, Topographic imaging of mine-Raland collagen in the calcifying turkey tendon[J]. Connect. Tissue Res, 1991,25:181-196.
[37]D. J. Prockop, A. Fertala, The collagen fibril: the almost crystalline structure[J]. Struct. Biol. 1998,122:111-18.
[38]D. R. Baselt, J. P. Revel, J. D. Baldeschwieler, Subfibrillar structure of type I collagen observed by atomicforce microscopy[J]. Biophys.1993, 65:2644-55.
[39]E. A. G.Chernoff, D. A. Chernoff, Atomic force microscope images of collagen fibers[J]. Vac. Sci. Technol. 1992, 10:596 - 99.
[40]T. J. Wess, J. P. Orgel, Changes in collagen structure: drying, dehy-drothermaltreatment and relation to long term deterioration[J]. Ther-mochim. Acta2000, 365: 119-28.
[41]J.Bella, B.Brodsky, Berman, Hydration structure of a collagen peptide[J]. Structure. 1995, 3:893 - 906.
[42]R. I. Price, S. Lees, D. A. Kirschner, X-ray diraction analysis of tendon collagen at ambient and cryogenic temperatures: role of hydration[J]. Biol. Macromol. 1997, 20:23 - 33.
[43]LarrabeeWF. A finite element model of skin deformation. I. Biomechanics of skin and soft tissue[J]. A review. Laryngoscope 1986,96:399-405.
[44] Pioletti DP, Rakotomanana LR. On the independence of time and strain effects on the stress relaxation of ligaments and tendons[J]. Biomech 2000,33:1729-32.
[45]Sasaki N, Shukunami N, Matsushima N, et al. Time-resolved X-ray diffraction from tendon collagen during creep using synchrotron radiation[J]. Biomech 1999,32:285 - 92.
[46]Craig R. G, Powers J.M. Restorative dental materials[M], 11th ed. St Louis: Mosby; 2002. pp. 94-7.
[47]Trengrove H. G, Carter G. M, Hood J. A. A, Stress relaxation properties of human dentin[J]. Dent Mater 1995,11:305-10.
[48]Balooch M, Wu-Magidi I-C, Balazs A, et al. Viscoelastic properties of demineralized dentin measured in water with atomic force microscope (AFM)-based indentation [J]. Biomed Mater Res ,1998,40:539-44.
[49]Nakabayashi N, Pashley DH. Hybridization of dental hard tissue[M]. Quintessence Publishing Co. 1998.
[50]Spencer P, Swafford JR. Unprotected protein at the dentin - adhesive interface[J]. Int J Quintessence 1999,30:501-7.
[51] Spencer P, Wang Y, Walker MP, et al. Interfacial chemistry of the dent in/adhesive bond [J]. Dent Res 2000,79:1458-63.
[52] David H. Pashley, Kelli A. Agee, John C. Wataha, et al. Viscoelastic properties of demineralized dentin matrix[J]. Dental Materials 2003, 19:700-706
[53]Duran R. L, Powers J. M, Craig R. G. Viscoelastic and dynamic properties of soft liners and tissue conditioners [J]. Dent Res. 1979,58:1801-7.
[54]Liu C.C,Wataha J.C,Craig R.G.The effect of repeated stretching on the force decay and compliance of vulcanized cis-polyisoprene orthodontic elastics[J].Dent Mater 1993,9:37-40.
[55]Ruyter IE,Oysaed H.Compressive creep in light cured resin based restorative materials[J].Acta Odont Scand 1982,40:319-24.
[56]Bowman SM,Gibson LJ,Hayes WC,et al.Results from demineralized bone creep tests suggest that collagen is responsible for the creep behavior of bone[J].Biomed Engng 1999,121:253-8.
[57]Marshall GW,Marshall SJ,Kinney JH,et al.The dentin substrate:structure and properties related to bonding[J].Dent 1997,25:441-58.
[58]Sasaki N,Nakayama Y,Yoshikawa M,et al.Stress-relaxation function in bone and bone collagen[J].J Biomech 1993,26:1369-76.
[59]费伟,李铮,胡静,等.贝复济(bFGF)促进拔牙创早期愈合的动物实验研究[J].中国口腔种植学杂志,2001,6(4):154-156.
[60]胡静,胡开进,费伟.bFGF对拔牙创内源性BMP含量及分布的影响[J].临床口腔医学杂志,1998,14(3):145-146.
[61]白志伟.羟基磷灰石预防复杂牙拔牙并发症的临床应用[J].中国航天工业医药,2001,3(5):54
[62]史剑杰,陈文雄.下颌阻生智齿拔牙创置入HAP对预防干槽症的临床观察[J].广州医学院学报,2000,28(2):68-70.
[63]Weiss P,Layrolle P,Clergeau LP,et al.The safety and efficacy of an injectable bone substitute in dental sockets demonstrated in a human clinical trial[J].Biomaterials,2007,28(22):3295-305.
[64]Kinoshita A,Oda S,Takahashi K,et al.Periodontal regeneration by application of recombinant human bone morphogenetic protein-2 to horizontal circumferential defects created by experimental periodontitis in beagle dogs[J].Periodontol,1997,68:103
[65]Sigurdsson TJ,Lee M,Kukota R,et al.Periodontal repair in dogs:recombinant human bone morphogenetic protein significantly enhances periodontal regeneration[J].Periodontol,1995,66(2):131-8
[66]Ferreira SD,Dernell WS,Powers BE,et al.Efficacy of gasplasma sterilization on the osteoinductive capacity of demineralized bone matrix[J].Clin Orthop,2001,38(8):233-9.
[67]胡晓波.骨诱导促进骨折愈合的实验研究[J]中华骨科杂志,1988,8(2):125-128.
[68]Arvalho VA,Tosello Dde O,Salgado MA,et al.Histomorphometric analysis of homogenous demineralized dentin matrix as osteopromotive material in rabbit mandibles[J].Oral Maxillofac Implants.2004,19(5):679-86.
[69]Gomes MF,dos Anjos MJS,Nogueira Tde O,et al.Autogenous demineralized dentin matrix for tissue engineering applications:radiographic and histomorphometric studies[J].Int J Oral Maxilofac Implants.2002,17(4):488-97.
[70]Goncalves EAL,Pavan AJ,Tavano O,et al.Atividade morfogenetica da matriz dentaria desmineralizada:estudoemcaes[J].Rev Fac Odontol Bauru.2002,10(1):51-6.
[71]Bessho K,Tagawa T,Murata M.Purification of rabbit bone morphogenetic protein derived from bone,dentin,and wound tissue after tooth extraction[J].J Oral Maxillofac Surg.1990,48(2):162-9.
[72]Carvalho VA,Tosello Dde O,Salgado MA,et al.Histomorphometric analysis of homogenous demineralized dentin matrix as osteopromotive material in rabbit mandibles[J].Int J Oral Maxillofac Implants.2004,19(5):679-86.
[73]Catanzaro-Guimaraes SA,Catanzaro-Guimaraes B,Garcia RB,et al.Osteogenic potential of autogenic demineralized dentin implanted in bony defects in dog[J].Int J Oral Maxillofac Surg.1986,15(2):160-9.
[74]Catanzaro-Guimaraes SA.Possibility to reinforce bone repair with decalcified dentin matrix[M].In:Gesellschaft f(u|¨)r orale Implantologie, editor. Jahrbuch fur oral Implantology. Berlin: Quintessenz. 1993. p.33-4.
[75]Gomes MF, Dos Anjos MJ, Nogueira TO, et al. Histologic evaluation of the osteoinductive property of autogenous demineralized dentin matrix on surgical bone defects in rabbit skulls using human amniotic membrane for guided bone regeneration[J]. Int J Oral Maxillofac Implants. 2001, 16 (4): 563-71.
[76]Gomes MF, Dos Anjos MJS, Nogueira Tde O, et al. Autogenous demineralized dentin matrix for tissue engineering applications: radiographic and histomorphometric studies[J]. Int J Oral Maxilofac Implants. 2002,17(4): 488-97.
[77]Goncalves EL, Catanzaro-Guimaraes SA. Proteinas morfogeneticas o sseas: terapeutica molecular no processo de reparo osseo[J]. Rev Odontol Univ Sao Paulo. 1998,12(3):299-304.
[78]Juodzbalys G, Raustia AM, Kubilius R. A 5-year follow-up study on one-stage implants inserted concomitantly with local ized alveolarridge augmentation[J].0ral Rehabil, 2007, 34(10):781-9.
[79]Silva FM, Cortez AL, Moreira RW, et al. Compl ications of intraoral donor site for bone grafting prior to implant placement[J]. Implant Dent, 2006, 15(4): 420-6.
[80]Powell CA, Mealey BL, Deas DE, et al. Post-surgical infections: prevalence associated with various periodontal surgical procedures [J]. Periodontol, 2005, 76(3):329-33.
[81]Mazzonetto R, Allais M, Maurette PE, et al. Vertical bone etrospective study of the potential complications during alveolar distraction osteogenesis in 55 patients[J]. Int J Oral Maxillofac Surg, 2007, 36(1): 6-10.
[82]Merli M, Migani M, Bernardelli F, et al. Vertical bone augmentation with dental implant placement: efficacy and complications associated with 2 different techniques,A retrospective cohort study[J].Int J Oral Maxillofac Implants,2006,21(4):600-6.
[83]Carinci F,Farina A,Zanetti U,et al.Alveolar ridge augmentation:a comparative longitudinal study between calvaria and iliac crest bone grafts[J].Oral Implantol,2005,31(1):39-45.
[84]谢迎,刘斌,岑远坤,李志革,董跟喜.种植体固定的钛网复合脱钙牙基质增高牙槽嵴的研究,中国修复重建外科杂志,2008,22(4):476.
[85]叶蜀新,谢小平,江伟,等.脱钙牙基质在骨修复中的应用[J].中国修复重建外科杂志,2002,16(5):348-350.
[86]刘斌,余占海,岑远坤,宋容脱钙牙基质改良三明治术增高牙槽嵴的研究[J],牙体牙髓牙周病学杂志 2004,14(10)563-566.
[87]朱政鸣,程凯敏,唐文渊,等.脱钙人牙基质诱导急性创伤性颅骨缺损一次性自体修复的实验及临床应用研究[J].重庆医学,2005,34(11):1623-1624.
[88]艾伟建,周磊.自体颏骨、Bio-Oss骨粉、胶原膜联合修复牙槽裂[J].广东牙病防治,2006,14(4):256-259.
[89]韩思源,王玉新,宋涛,等.自体髂软骨及松质骨移植在同期行鼻唇畸形整复术中的应用.中华整形外科杂志,2004,20(4):248-251.
[90]陆斌,王彦亮,孙沫逸,等.牙槽裂植骨术后的临床分级及其影响因素的探讨[J].临床口腔医学杂志,2004,20(8):492-494.
[91]王鑫,罗弈.牙槽嵴裂骨移植修复术后影响骨吸收因素的初步研究[J].中华口腔医学杂志,2005,40(5):373-375.
[92]张雷,孙志干,霍永力.自体骨移植在修复齿槽突裂中的应用研究.宁夏医学杂志[J],2001,23(6):339-340.
[93]万延俊,吴刚,张红闯.采用髂骨骨松质行齿槽嵴裂植骨46例临床分析[J].口腔医学,2004,24(6):357-358.
[94]华培生,李成林.脱钙人牙基质骨诱导的研究[J].中华实验外科杂志,1997,14(5):301.
[95]王鹏来,赵士芳,刘超,等.骨移植术修复单侧唇腭裂鼻翼基底塌陷畸形.现代口腔医学杂志,2003,17(1):87.
[96]王哲,王全平,李新奎,等.胸椎黄韧带骨化与腰椎黄韧带退变的病理及在本代谢元素的比较研究[J].中华骨科杂志,1998,18(11):656-658.
[97]Okada K,Oka S,Tohge K.Thoracic myelopathy caused by ossification of the ligamentum flavum[J].Spine,1991,16(3):278.
[98]王哲,王全平,李新奎,等.胸椎黄韧带骨化症的诊断与治疗[J].中国矫形外科杂志,1998,5(2):104-106.
[99]Maigne JY,Ayral X.Frequency and size of ossification in the caudal attachments of the ligamentum flavum of the thoracis spine[J].Surg Radio Anat,1992,14(2):119.
[100]袁志,马平,胡蕴玉,等.复合基因重组人骨形成蛋白—2异种骨的研制及骨活性研究[J].中国修复重建外科杂志,2002,16(2):79
[101]Urist MR,Strates BS.Bone morphogenetic protein[J].Dent Res.1971,50:1392-1406
[102]Kawai T,Urist MR.A bovine tooth derived bone morphogenetic protein[J].Dent Res,1989,68:1069-74
[103]K.Yagihashi,K.Miyazawa,K.Togari,et al.Demineralized Dentin Matrix Acts as a Scaffold for Repair of Articular Cartilage Defects[J].Int J Calcif Tissue,2009,84:210-20.
[104]Wakitani S,Imoto K,Kimura T,et al.Hepatocyte growth factor facilitates cartilage repair:full thickness articular cartilage defect studied in rabbit knees[J].Acta Orthop Scand.1997,68:474-80.
[105]Minns RJ,Muckle DS,Donkin JE.The repair of osteo chondral defects in osteoarthritic rabbit knees by the use of carbon fibre[J].Biomaterials 1982,3:81-86.
[106]Messner K,Gillquist J.Synthetic implants for the repair of osteochondral defects of the medial femoral condyle:a biomechanical and histological evaluation in the rabbit knee[J].Biomaterials 1993,14:513-21.
[107]VonSchroeder HP,Kwan M,Amiel D,et al.The use of polylactic acid matrix and periosteal grafts for the reconstruction of rabbit knee articular defects[J]. Biomed Mater Res 1991,25:329-39.
[108]Erkki S, Allan JA, Erik V, et al. Subchondral bone and cartilage repair with bioactive glasses, hydroxyapatite, and hydroxyapatite-glass composite[J]. Biomed Mater Res. 1996, 32:543-51.
[109]Stephen D, PhD Cook, Laura P, et al. Repair of articular cartilage defects with osteogenic protein - 1 (BMP-1) in dogs[J]. Bone Joint Surg Am. 2003, 85:116-23.
[110] Tamai N, Myoui A, Hirao M, et al.A new biotechnology for articular cartilage repair: subchondral implantation of a composite of interconnected porous hydroxyapatite, synthetic polymer (PLA-PEG), and bone morphogenetic protein-2(rhBMP-2)[J]. Osteoarth Cartilage. 2005,13: 405-17.
[2]Cochran DL, Schenk R, Buser D, et al. Corrosion behaviour of commercially pure titanium shot blasted with different materials and sizes of shot particles for dental implant applications[J]. Periodontol, 1999, 70: 139-50.
[3]Urist MR, Delange RJ, Finerman GAM. Bone cell differentiation and growth factors induced activity of chondroosteogenic DNA [J]. Science. 1983, 220(4598):680-6
[4]Urist MR, Selang RH. Methods of preparation and bioassay of bone morphogenetic protein and polypeptide fragments [M]. Barnes D, Sirbaska DA, eds. Methods in Enzymology. vol. 146. New York:Academy Press, 1987: 294.
[5]Bergsma JE, Rozema FR , Bos RR, et al. Biocompatibility study of as polymetized poly(L-lactide) in rats using a cage implant system[J]. Biomed Mater Res ,1995 ,29 (2) :173 - 9
[6]Wheater PH, Burkitt HG, Daniels VG. Functional histology[M]. London:Churchill livingstone, 1982: 128
[7] Urist MR. Experimental delivery systems for bone morphogenetic protein. In:Wise DL, eds. Hand book of Biomaterials and Applications, Section Orthopaedic Biomaterials Applications[J]. Boston , Mass : Marcel Dekker ,1995,3:1093-133.
[8]Urist MR, Lietze A, Mizutani H, et al. A bovine low molecular weight bone morphogenetic protein (BMP) fraction[J].Clin Orthop, 1982,162:219
[9]Kinoshita A, Oda S, Takahashi K, et al. Periodontal regeneration by application of recombinant human bone morphogenetic protein-2 to horizontal circumferential defects created by experimental periodontitis in beagle dogs[J]. Periodontol, 1997, 68(2):103-9
[10]Sigurdsson TJ, Lee M, Kukota R, et al. Periodontal repair in dogs: recombinant human bone morphogenetic protein significantly enhances periodontal regeneration[J].Periodontol,1995,66(2):131-8
[11]岑远坤,李文,廖运茂,等.脱钙牙本质基质及其复合物整复节段性骨缺损的组织学观察[J].华西口腔医学杂志,1998;16(3):263-265
[12]杨连甲,金岩,胡蕴玉主编.口腔和骨科的生物活性材料[M].西安:陕西科学技术出版社,1993:141-231.
[13]Merry J.C,Gibsm I.R,Best S.M,et al.Synthesis and characterization of carbonate hydroxyapatite[J].Mater Sci Mater Med,1998,12:779-83.
[14]Azic S,Popovic J K,Zec S,et al.Properties of hydroxyapatitellized from high temperature alkaline solutions[J].Cryst Growth.1996,165:124-8.
[15]Shirkhanzadeh M.Bioactive calcium phosphate coatingsprepared by electrodeposition[J].Mater Sci Lett,1991,10:1415-17.
[16]李世普.生物医用材料导论[M].武汉:武汉工业大学出版社.2000(8):88-123.
[17]Suchanek W,Y oshimura M.Processing and properties of hydroxyapatite 2based biomaterials for use as hard tissue replacement implants[J].Mater Res,1998,13:94-117.
[18]Kumar R,Prakash KH,Cheang P,et al.Temperature driven morphological changes of chemically precipitated hydroxyapatite nanoparticles[J].Langmuir.2004,20(13):5196-200.
[19]Liu DM,Yang Q,Troczynski T,et al.Structural evolution of sol-gel-derived hydroxyapatite[J].Biomaterials 2002,23(7):1679-87.
[20]Adkisson H.D,Strauss-Schoenberger J,Gillis M,Rapid quantitative bioassay of osteoinduction[J].Orthop Res.2000,18(3):503-11.
[21]Aaron RK,Ciombor DM.Acceleration of experimental endochondral ossification by biophysical stimulation of the progenitor cell pool[J].Orthop Res,1996,14(4):582.
[22]程玉华,赵卜军,刘海俊,等.胶原复合羟基磷灰石修复下颌骨缺损的组 织学观察[J].中国修复重建外科杂志,1998,12(2):74
[23]刘斌.种植体固定增高牙槽嵴的动物实验研究.[P].华西医科大学硕士学位论文,2000;
[24]Mosman T.Rapid colorimetric assay for cellular growth and survival:Application to proliferation and cytotoxicity assays[J].Immunol Methods,1983,65(1-2):55-63.
[25]Sjogren G,Sletten G,Dahl J.E,Cytotoxicity of dental alloys,metals,and ceramics assessed by Millipore filter,agar overlay,and MTT tests[J].Journal of Prosthetic Dentistry,2000,84(2):229-36.
[26]Morrison C,Macnair R,Macdonald C,et al.In Vitro Biocompatibility Testing of Polymers for Orthopedic Implants Using Cultured Fibroblasts and Osteoblasts[J].Biomaterials,1995,16(13):987-92.
[27]Timmer M.D,Shin H,Horch R.A,et al.In vitro cytotoxicity of injectable and biodegradable poly(propylene fumarate)-basednetworks:Unreacted macromers,cross-linked networks,and degradation products[J].Biomacromolecules,2003,4(4):1026-33.
[28]Thonemann B,Schmalz G,Hiller K.A,et al.Responses of L929 mouse fibroblasts,primary and immortalized bovine dental papilla-derived cell lines to dental resin components[J].Dental Materials,2002,18(4):318-23.
[29]Herbertson A,Aubin JE.Cell sorting entices osteogenic populations in rat bonemarrow stromal cell cultures[J].Bone,1997,21(6):491
[30]Aubin J.E,Advances in the osteoblast lineage[J].Biochem Cell Biol,1998,76(6):899-910.
[31]Takahashi Y,Yamamoto M,Tabata Y.Osteogenic differentiation of mesenchymal step cells in biodegradable sponges composed of gelatin and beta-tricalcium phosphate[J].Biomaterials,2005,26(17):3587-96.
[1]Urist MR,hietze A,Mizutani H,et al.A bovine low molecular weight bone morphogenetic protein(BMP) fraction[J].Clin Orthop.1982,162:219.
[2]Aaron RE,Ciombor DM.Acceleration of experimental endochondral ossi-Fication by biophysical stimulation of the progenitor cell pool[J].Orthop Res,1996,14(4):582.
[3]程玉华,赵卜军,刘海俊,等.胶原复合羟基磷灰石修复下颌骨缺损的组织学观察[J].中国修复重建外科杂志,1998,12(2):74
[4]李起鸿.我国修复长骨大段骨缺损的进展[J].中华骨科杂志,1997,17(1):13
[5]岑远坤,李文,廖运茂,等.脱钙牙本质基质及其复合物整复节段性骨缺损的组织学观察[J].华西口腔医学杂志,1998,16(3):263
[6]杨连甲,金岩,胡蕴玉主编.口腔和骨科的生物活性材料[M].西安:陕西科学技术出版社,1993:141-231.
[7]Zehnder M,Waltimo N,Sener B,et al.Dentin enhances the effectiveness of bioactive glass S53P4 against a strain of Enterococcus faecalis[J].Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics,2006,101(4):530-5.
[8]Zhang Y,Agee KNor J,et al.Effects of acid-etching on the tensile properties of demineralized dentin matrix[J].Dental Materials,1998,14(3):222-228.
[9]C.Begue-Kirn,P.H.Krebsbach,J.D.Bartlett,et al.Dentin sialoprotein,dentin phosphoprotein,enamelysin and ameloblastin:tooth-specific molecules that are distinctively expressed during murine dental dierentiation [J].Oral Sci.1998,106:963-70.
[10]M.E.Nimni,R.D.Harkness,Molecular structures and functions of collagens[M].Nimni,M.E.(Ed.),Collagen:vol.Ⅰ,Biochemistry.CRC Press,Boca Raton,FL,1988,pp.1-79.
[11]D.A.D.Parry,A.S.Craig,Collagen fibrils during development[M].In:Nimni,M.E.(Ed.),Collagen.CRC Press,Boca Raton,FL,1988,pp.1-20.
[12]B.Brodsky,S.Tanaka,E.F.Eikenberry,et al.X-ray diffraction as a tool for studying collagen structure[M]. In: Nimni, M. E. (Ed.), Collagen: vol. I, Biochemistry. CRC Press, Boca Raton, FL, p. 5v.
[13]R. Garberoglio, M. Brannstorm, Scanning electron microscopic investigation of human dentinal tubules[J]. Arch.Oral Biol. 1976,21:355-62.
[14]D. H. Pashley, Clinical correlations of dentin structure and function [J]. Prosthet. Dent. 1991, 66:777-81.
[15] J. Perdigao, P. Lambrechts, B. Van Meerbeek, et al. Morphological field emission - SEM study of the elect of six phosphoric acid etching agents on human dentin[J]. Am. J. Dent. Mater. 1996, 12:262-71.
[16] J. K. Avery, Oral Development and Histology B.C. Dekker Toronto[M], Philadelphia. 1988.
[17]C. P. Lin, W. H. Douglas, S. L. Erlandsen, Scanning electron microscopy of type I collagen at the dentin - enamel junction of human teeth[J]. Histochem Cytochem. 1993,41:381-88.
[18]A. L. Arsenault, A comparative electron microscopic study of apatite crystals in collagen fibrils of rat bone, dentin and calcified turkey leg tendons[J]. Bone Miner. 1989,6:165-77.
[19] J. Perdigao, J.Y.Thompson, M. Toledano, et al. Anultra-morphological characterization of collagen-depleted etched dentin [J]. Am J Dent, 1999. 12:250-55.
[20]K. D. Moses, W. T. Butler, C. Qin, Immunohistochemical study of small integrin-binding ligand, N-linked glycoproteins in reactionary dentin of rat molars at dierent ages[J]. Oral. Sci. 2006,114:216-22.
[21] LF. Massa, A. Ramachandran, A.George, et al. Developmental appearance of dentin matrix protein 1 during the early dentinogenesis in rat molars as identified by high-resolution immunocytochemistry, Histochem[J]. Cell Bio2005,124:197-205.
[22] R. N. D Souza, A. Cavender, G, Sunavala , et al. Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo[J]. Bone Miner Res. 1997,12:2040-49.
[23]N. Kamiya, M. Takagi, Differential, Expression of dentin matrix protein 1, type I collagen and osteocalcin genes in rat developing mandibular bone[J].Histochem 2001,33:545-52.
[24] J.Q.Feng, H.Huang, Y. Lu, et al. The Dentinmatrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development[J]. Dent Res. 2003, 82:776-80.
[25]P. H. Tartaix, M. Doulaverakis, A. George, et al. In vitro effects of dentinmatrix protein-1 on hydroxyapatite formation provide insights into in vivo functions[J].Biol Chem. 2004,179:18115-20.
[26]G. He, T. Dahl, A. Veis, et al. Dentin matrix protein 1 initiates hydroxyapatite formation in vitro [J]. Connect Tissue Res. 2003, 44:240-45.
[27] J. L. Pang, B. L. Wu, W. X. He, et al. Effect of antisense oligonucleotide against mouse dentine matrix protein 1 on mineralization ability and calcium ions metabolism in odontoblast-like cell line MDPC-23[J]. Endod. 2006,39:527-37.
[28]G. He, S. Gajjeraman, D. Schultz, et al. Spatially and temporally controlled biomineral-ization is facilitated by interaction between self-assembled dentin matrix protein 1 and calcium phosphate nuclei in solution[J]. Biochemistry , 2005, 44:16140 - 48.
[29]A. Almushayt, K. Narayanan, A. E. Zaki, et al. Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odonto-blasts, Gene[J]. Ther. 2006, 13:611-20.
[30]K. Narayanan, R. Srinivas, A. Ramachandran, et al. Differentiation of embryonic mesenchymal cells to odonto-blast-like cells by over-expression of dentin matrix protein 1, Proc. Natl[J]. Acad. Sci. USA 2001, 98:4516-21.
[31]Stefan Habelitz, Mehdi Balooch, Sally J. Marshall, et al. In situ atomic force microscopy of partially demineralized human dentin collagen fibrils[J]. Journal of Structural Biology, 2002,138:227-36.
[32] J. H. Kinney, D. L.Haupt,M. Balooch, et al. The threshold effects of Nd and Ho: YAG laser-induced surface modification on demineralization of dentin surfaces[J]. Dent Res, 1996, 75:1388-95.
[33]G. W.Marshall, S.J.Marshall, J. H. Kinney, et al. The dentin substrate: structure and properties related to bonding[J]. Dent. 1997, 25:441-58.
[34] F. El Feninat, T.H.Ellis, E. Sacher, et al. A tapping mode AFM study of collapse and denaturation in dentinal collagen[J]. Dent. Mater. 2001, 17:284-8.
[35]E. Baer, J. J. Cassidy, A. Hiltner, Hierachical structure of collagen and its relationship to the physical properties of tendon[M]. In: Nimni, M. E. (Ed.), Collagen: vol. II, Biochemistry and Biomechanics. CRC Press, Boca Raton, FL, p. 5v. 1988.
[36]W. J. Landis, J.Moradian-Oldak, S.Weiner, Topographic imaging of mine-Raland collagen in the calcifying turkey tendon[J]. Connect. Tissue Res, 1991,25:181-196.
[37]D. J. Prockop, A. Fertala, The collagen fibril: the almost crystalline structure[J]. Struct. Biol. 1998,122:111-18.
[38]D. R. Baselt, J. P. Revel, J. D. Baldeschwieler, Subfibrillar structure of type I collagen observed by atomicforce microscopy[J]. Biophys.1993, 65:2644-55.
[39]E. A. G.Chernoff, D. A. Chernoff, Atomic force microscope images of collagen fibers[J]. Vac. Sci. Technol. 1992, 10:596 - 99.
[40]T. J. Wess, J. P. Orgel, Changes in collagen structure: drying, dehy-drothermaltreatment and relation to long term deterioration[J]. Ther-mochim. Acta2000, 365: 119-28.
[41]J.Bella, B.Brodsky, Berman, Hydration structure of a collagen peptide[J]. Structure. 1995, 3:893 - 906.
[42]R. I. Price, S. Lees, D. A. Kirschner, X-ray diraction analysis of tendon collagen at ambient and cryogenic temperatures: role of hydration[J]. Biol. Macromol. 1997, 20:23 - 33.
[43]LarrabeeWF. A finite element model of skin deformation. I. Biomechanics of skin and soft tissue[J]. A review. Laryngoscope 1986,96:399-405.
[44] Pioletti DP, Rakotomanana LR. On the independence of time and strain effects on the stress relaxation of ligaments and tendons[J]. Biomech 2000,33:1729-32.
[45]Sasaki N, Shukunami N, Matsushima N, et al. Time-resolved X-ray diffraction from tendon collagen during creep using synchrotron radiation[J]. Biomech 1999,32:285 - 92.
[46]Craig R. G, Powers J.M. Restorative dental materials[M], 11th ed. St Louis: Mosby; 2002. pp. 94-7.
[47]Trengrove H. G, Carter G. M, Hood J. A. A, Stress relaxation properties of human dentin[J]. Dent Mater 1995,11:305-10.
[48]Balooch M, Wu-Magidi I-C, Balazs A, et al. Viscoelastic properties of demineralized dentin measured in water with atomic force microscope (AFM)-based indentation [J]. Biomed Mater Res ,1998,40:539-44.
[49]Nakabayashi N, Pashley DH. Hybridization of dental hard tissue[M]. Quintessence Publishing Co. 1998.
[50]Spencer P, Swafford JR. Unprotected protein at the dentin - adhesive interface[J]. Int J Quintessence 1999,30:501-7.
[51] Spencer P, Wang Y, Walker MP, et al. Interfacial chemistry of the dent in/adhesive bond [J]. Dent Res 2000,79:1458-63.
[52] David H. Pashley, Kelli A. Agee, John C. Wataha, et al. Viscoelastic properties of demineralized dentin matrix[J]. Dental Materials 2003, 19:700-706
[53]Duran R. L, Powers J. M, Craig R. G. Viscoelastic and dynamic properties of soft liners and tissue conditioners [J]. Dent Res. 1979,58:1801-7.
[54]Liu C.C,Wataha J.C,Craig R.G.The effect of repeated stretching on the force decay and compliance of vulcanized cis-polyisoprene orthodontic elastics[J].Dent Mater 1993,9:37-40.
[55]Ruyter IE,Oysaed H.Compressive creep in light cured resin based restorative materials[J].Acta Odont Scand 1982,40:319-24.
[56]Bowman SM,Gibson LJ,Hayes WC,et al.Results from demineralized bone creep tests suggest that collagen is responsible for the creep behavior of bone[J].Biomed Engng 1999,121:253-8.
[57]Marshall GW,Marshall SJ,Kinney JH,et al.The dentin substrate:structure and properties related to bonding[J].Dent 1997,25:441-58.
[58]Sasaki N,Nakayama Y,Yoshikawa M,et al.Stress-relaxation function in bone and bone collagen[J].J Biomech 1993,26:1369-76.
[59]费伟,李铮,胡静,等.贝复济(bFGF)促进拔牙创早期愈合的动物实验研究[J].中国口腔种植学杂志,2001,6(4):154-156.
[60]胡静,胡开进,费伟.bFGF对拔牙创内源性BMP含量及分布的影响[J].临床口腔医学杂志,1998,14(3):145-146.
[61]白志伟.羟基磷灰石预防复杂牙拔牙并发症的临床应用[J].中国航天工业医药,2001,3(5):54
[62]史剑杰,陈文雄.下颌阻生智齿拔牙创置入HAP对预防干槽症的临床观察[J].广州医学院学报,2000,28(2):68-70.
[63]Weiss P,Layrolle P,Clergeau LP,et al.The safety and efficacy of an injectable bone substitute in dental sockets demonstrated in a human clinical trial[J].Biomaterials,2007,28(22):3295-305.
[64]Kinoshita A,Oda S,Takahashi K,et al.Periodontal regeneration by application of recombinant human bone morphogenetic protein-2 to horizontal circumferential defects created by experimental periodontitis in beagle dogs[J].Periodontol,1997,68:103
[65]Sigurdsson TJ,Lee M,Kukota R,et al.Periodontal repair in dogs:recombinant human bone morphogenetic protein significantly enhances periodontal regeneration[J].Periodontol,1995,66(2):131-8
[66]Ferreira SD,Dernell WS,Powers BE,et al.Efficacy of gasplasma sterilization on the osteoinductive capacity of demineralized bone matrix[J].Clin Orthop,2001,38(8):233-9.
[67]胡晓波.骨诱导促进骨折愈合的实验研究[J]中华骨科杂志,1988,8(2):125-128.
[68]Arvalho VA,Tosello Dde O,Salgado MA,et al.Histomorphometric analysis of homogenous demineralized dentin matrix as osteopromotive material in rabbit mandibles[J].Oral Maxillofac Implants.2004,19(5):679-86.
[69]Gomes MF,dos Anjos MJS,Nogueira Tde O,et al.Autogenous demineralized dentin matrix for tissue engineering applications:radiographic and histomorphometric studies[J].Int J Oral Maxilofac Implants.2002,17(4):488-97.
[70]Goncalves EAL,Pavan AJ,Tavano O,et al.Atividade morfogenetica da matriz dentaria desmineralizada:estudoemcaes[J].Rev Fac Odontol Bauru.2002,10(1):51-6.
[71]Bessho K,Tagawa T,Murata M.Purification of rabbit bone morphogenetic protein derived from bone,dentin,and wound tissue after tooth extraction[J].J Oral Maxillofac Surg.1990,48(2):162-9.
[72]Carvalho VA,Tosello Dde O,Salgado MA,et al.Histomorphometric analysis of homogenous demineralized dentin matrix as osteopromotive material in rabbit mandibles[J].Int J Oral Maxillofac Implants.2004,19(5):679-86.
[73]Catanzaro-Guimaraes SA,Catanzaro-Guimaraes B,Garcia RB,et al.Osteogenic potential of autogenic demineralized dentin implanted in bony defects in dog[J].Int J Oral Maxillofac Surg.1986,15(2):160-9.
[74]Catanzaro-Guimaraes SA.Possibility to reinforce bone repair with decalcified dentin matrix[M].In:Gesellschaft f(u|¨)r orale Implantologie, editor. Jahrbuch fur oral Implantology. Berlin: Quintessenz. 1993. p.33-4.
[75]Gomes MF, Dos Anjos MJ, Nogueira TO, et al. Histologic evaluation of the osteoinductive property of autogenous demineralized dentin matrix on surgical bone defects in rabbit skulls using human amniotic membrane for guided bone regeneration[J]. Int J Oral Maxillofac Implants. 2001, 16 (4): 563-71.
[76]Gomes MF, Dos Anjos MJS, Nogueira Tde O, et al. Autogenous demineralized dentin matrix for tissue engineering applications: radiographic and histomorphometric studies[J]. Int J Oral Maxilofac Implants. 2002,17(4): 488-97.
[77]Goncalves EL, Catanzaro-Guimaraes SA. Proteinas morfogeneticas o sseas: terapeutica molecular no processo de reparo osseo[J]. Rev Odontol Univ Sao Paulo. 1998,12(3):299-304.
[78]Juodzbalys G, Raustia AM, Kubilius R. A 5-year follow-up study on one-stage implants inserted concomitantly with local ized alveolarridge augmentation[J].0ral Rehabil, 2007, 34(10):781-9.
[79]Silva FM, Cortez AL, Moreira RW, et al. Compl ications of intraoral donor site for bone grafting prior to implant placement[J]. Implant Dent, 2006, 15(4): 420-6.
[80]Powell CA, Mealey BL, Deas DE, et al. Post-surgical infections: prevalence associated with various periodontal surgical procedures [J]. Periodontol, 2005, 76(3):329-33.
[81]Mazzonetto R, Allais M, Maurette PE, et al. Vertical bone etrospective study of the potential complications during alveolar distraction osteogenesis in 55 patients[J]. Int J Oral Maxillofac Surg, 2007, 36(1): 6-10.
[82]Merli M, Migani M, Bernardelli F, et al. Vertical bone augmentation with dental implant placement: efficacy and complications associated with 2 different techniques,A retrospective cohort study[J].Int J Oral Maxillofac Implants,2006,21(4):600-6.
[83]Carinci F,Farina A,Zanetti U,et al.Alveolar ridge augmentation:a comparative longitudinal study between calvaria and iliac crest bone grafts[J].Oral Implantol,2005,31(1):39-45.
[84]谢迎,刘斌,岑远坤,李志革,董跟喜.种植体固定的钛网复合脱钙牙基质增高牙槽嵴的研究,中国修复重建外科杂志,2008,22(4):476.
[85]叶蜀新,谢小平,江伟,等.脱钙牙基质在骨修复中的应用[J].中国修复重建外科杂志,2002,16(5):348-350.
[86]刘斌,余占海,岑远坤,宋容脱钙牙基质改良三明治术增高牙槽嵴的研究[J],牙体牙髓牙周病学杂志 2004,14(10)563-566.
[87]朱政鸣,程凯敏,唐文渊,等.脱钙人牙基质诱导急性创伤性颅骨缺损一次性自体修复的实验及临床应用研究[J].重庆医学,2005,34(11):1623-1624.
[88]艾伟建,周磊.自体颏骨、Bio-Oss骨粉、胶原膜联合修复牙槽裂[J].广东牙病防治,2006,14(4):256-259.
[89]韩思源,王玉新,宋涛,等.自体髂软骨及松质骨移植在同期行鼻唇畸形整复术中的应用.中华整形外科杂志,2004,20(4):248-251.
[90]陆斌,王彦亮,孙沫逸,等.牙槽裂植骨术后的临床分级及其影响因素的探讨[J].临床口腔医学杂志,2004,20(8):492-494.
[91]王鑫,罗弈.牙槽嵴裂骨移植修复术后影响骨吸收因素的初步研究[J].中华口腔医学杂志,2005,40(5):373-375.
[92]张雷,孙志干,霍永力.自体骨移植在修复齿槽突裂中的应用研究.宁夏医学杂志[J],2001,23(6):339-340.
[93]万延俊,吴刚,张红闯.采用髂骨骨松质行齿槽嵴裂植骨46例临床分析[J].口腔医学,2004,24(6):357-358.
[94]华培生,李成林.脱钙人牙基质骨诱导的研究[J].中华实验外科杂志,1997,14(5):301.
[95]王鹏来,赵士芳,刘超,等.骨移植术修复单侧唇腭裂鼻翼基底塌陷畸形.现代口腔医学杂志,2003,17(1):87.
[96]王哲,王全平,李新奎,等.胸椎黄韧带骨化与腰椎黄韧带退变的病理及在本代谢元素的比较研究[J].中华骨科杂志,1998,18(11):656-658.
[97]Okada K,Oka S,Tohge K.Thoracic myelopathy caused by ossification of the ligamentum flavum[J].Spine,1991,16(3):278.
[98]王哲,王全平,李新奎,等.胸椎黄韧带骨化症的诊断与治疗[J].中国矫形外科杂志,1998,5(2):104-106.
[99]Maigne JY,Ayral X.Frequency and size of ossification in the caudal attachments of the ligamentum flavum of the thoracis spine[J].Surg Radio Anat,1992,14(2):119.
[100]袁志,马平,胡蕴玉,等.复合基因重组人骨形成蛋白—2异种骨的研制及骨活性研究[J].中国修复重建外科杂志,2002,16(2):79
[101]Urist MR,Strates BS.Bone morphogenetic protein[J].Dent Res.1971,50:1392-1406
[102]Kawai T,Urist MR.A bovine tooth derived bone morphogenetic protein[J].Dent Res,1989,68:1069-74
[103]K.Yagihashi,K.Miyazawa,K.Togari,et al.Demineralized Dentin Matrix Acts as a Scaffold for Repair of Articular Cartilage Defects[J].Int J Calcif Tissue,2009,84:210-20.
[104]Wakitani S,Imoto K,Kimura T,et al.Hepatocyte growth factor facilitates cartilage repair:full thickness articular cartilage defect studied in rabbit knees[J].Acta Orthop Scand.1997,68:474-80.
[105]Minns RJ,Muckle DS,Donkin JE.The repair of osteo chondral defects in osteoarthritic rabbit knees by the use of carbon fibre[J].Biomaterials 1982,3:81-86.
[106]Messner K,Gillquist J.Synthetic implants for the repair of osteochondral defects of the medial femoral condyle:a biomechanical and histological evaluation in the rabbit knee[J].Biomaterials 1993,14:513-21.
[107]VonSchroeder HP,Kwan M,Amiel D,et al.The use of polylactic acid matrix and periosteal grafts for the reconstruction of rabbit knee articular defects[J]. Biomed Mater Res 1991,25:329-39.
[108]Erkki S, Allan JA, Erik V, et al. Subchondral bone and cartilage repair with bioactive glasses, hydroxyapatite, and hydroxyapatite-glass composite[J]. Biomed Mater Res. 1996, 32:543-51.
[109]Stephen D, PhD Cook, Laura P, et al. Repair of articular cartilage defects with osteogenic protein - 1 (BMP-1) in dogs[J]. Bone Joint Surg Am. 2003, 85:116-23.
[110] Tamai N, Myoui A, Hirao M, et al.A new biotechnology for articular cartilage repair: subchondral implantation of a composite of interconnected porous hydroxyapatite, synthetic polymer (PLA-PEG), and bone morphogenetic protein-2(rhBMP-2)[J]. Osteoarth Cartilage. 2005,13: 405-17.