黄芩苷复合MTA用于犬实验性牙髓炎盖髓后TNF-α表达的研究
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摘要
目的
本研究通过脂多糖(lipopolysaccharide, LPS)诱导法建立犬实验性牙髓炎模型,然后分别采用黄芩苷海藻酸钠凝胶复合矿物三氧化聚合体(mineral trioxide aggregate, MTA)、Dycal和MTA行直接盖髓术,于不同时间点进行免疫组化检测TNF-α表达,以评估动物模型盖髓治疗效果,研究结果将为黄芩苷海藻酸钠凝胶复合MTA临床治疗牙髓炎提供实验依据和理论基础。
方法
实验选用2只健康beagle犬(年龄为12～14个月),SPF级,随机分为7天组、30天组,每组1只。每只beagle犬用LPS诱导法制备牙髓炎动物模型,然后采用黄芩苷海藻酸钠凝胶复合MTA进行盖髓治疗,并设Dycal、MTA盖髓组作为对照。术后7天、30天经颈动脉灌注固定处死动物,常规制作石蜡切片,进行免疫组化检测盖髓前及盖髓后各组牙髓组织中肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)表达的变化。
结果
1盖髓后7天,黄芩苷海藻酸钠凝胶复合MTA组TNF-α阳性细胞率低于牙髓炎、Dycal组和MTA组(P<0.05),高于正常牙髓(P<0.01)；MTA组TNF-α阳性细胞率低于牙髓炎(P<0.05)；Dycal组TNF-α阳性细胞率明显高于正常牙髓(P<0.01)。
2盖髓后30天,黄芩苷海藻酸钠凝胶复合MTA组TNF-α阳性细胞率明显低于牙髓炎、Dycal组和MTA组(P<0.05)；MTA组TNF-α阳性细胞率高于正常牙髓(P<0.01),低于牙髓炎、Dycal组(P<0.05)；Dycal组TNF-α阳性细胞率高于正常牙髓(P<0.01),低于牙髓炎(P<0.01)。
结论
1 LPS法诱导犬实验性局限性牙髓炎简便可行,并能很好控制牙髓炎症程度。
2黄芩苷海藻酸钠凝胶复合MTA用于牙髓炎直接盖髓术,与氢氧化钙(Dycal)、MTA相比,TNF-a阳性细胞率减少,结果提示黄芩苷复合MTA可作为治疗牙髓炎的方法之一。
Objective
The purpose of this study was to establish an experimental model of beagle dogs pulpitis induced by lipopolysaccharide (LPS).The exposed pulps were capped with three different agents: baicalin sodium alginate gel combined with mineral trioxide aggregate (MTA), Dycal,MTA.The therapeutic effects of direct capping were assessed through immunohistochemistry at different time point. The result of this study will provide experimental evidences and theoretical basises for using baicalin sodium alginate gel combined with MTA to cure pulpitis.
Methods
Two healthy, SPF level beagle dogs(age:12～14 months) were randomly divided into two groups,which are 7 days group and 30 days group.Each beagle dog was established an experimental model of pulpitis induced by LPS. A therapy of pulp capping will be processed with baicalin sodium alginate gel combined with MTA. Dycal,MTA were used as pulp capping agents in control groups.The dogs were sacrificed at 7th day and 30th day after arteria carotis perfusion fixation. Taking out the experimental teeth to make the paraffin and thinest slices. Immunohistochemical stains were performed to identify the change of tumor necrosis factor-a(TNF-a)expression before and after pulp capping.
Results
1 At 7th day after pulp capping, the rate of TNF-a positive cells in baicalin sodium alginate gel combined with MTA group was lower than those in pulpitis, Dycal group and MTA group(P<0.05),higher than those in normal pulp(P<0.01).The rate of TNF-a positive cells in MTA group was lower than those in pulpitis (P<0.05).The rate of TNF-a positive cells in Dycal group was obviously higher than those in normal pulp(P<0.01).
2 At 30th day after pulp capping, the rate of TNF-a positive cells in baicalin sodium alginate gel combined with MTA group was lower than those in pulpitis, Dycal group and MTA group(P<0.05). The rate of TNF-a positive cells in MTA group was higher than those in normal pulp (P＜0.01), lower than those in pulpitis, Dycal group (P<0.05).The rate of TNF-a positive cells in Dycal group was higher than those in normal pulp (P<0.01), lower than those in pulpitis (P<0.01).
Conclusions
1 The method to establish an limited experimental model of beagle dog pulpitis induced by LPS was feasible.
2 It had better effects using baicalin sodium alginate gel combined with MTA as pulp capping agents in pulptis than Dycal or MTA, with having advantage of reducing the expression of TNF-a.
本研究通过脂多糖(lipopolysaccharide, LPS)诱导法建立犬实验性牙髓炎模型,然后分别采用黄芩苷海藻酸钠凝胶复合矿物三氧化聚合体(mineral trioxide aggregate, MTA)、Dycal和MTA行直接盖髓术,于不同时间点进行免疫组化检测TNF-α表达,以评估动物模型盖髓治疗效果,研究结果将为黄芩苷海藻酸钠凝胶复合MTA临床治疗牙髓炎提供实验依据和理论基础。
方法
实验选用2只健康beagle犬(年龄为12～14个月),SPF级,随机分为7天组、30天组,每组1只。每只beagle犬用LPS诱导法制备牙髓炎动物模型,然后采用黄芩苷海藻酸钠凝胶复合MTA进行盖髓治疗,并设Dycal、MTA盖髓组作为对照。术后7天、30天经颈动脉灌注固定处死动物,常规制作石蜡切片,进行免疫组化检测盖髓前及盖髓后各组牙髓组织中肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)表达的变化。
结果
1盖髓后7天,黄芩苷海藻酸钠凝胶复合MTA组TNF-α阳性细胞率低于牙髓炎、Dycal组和MTA组(P<0.05),高于正常牙髓(P<0.01)；MTA组TNF-α阳性细胞率低于牙髓炎(P<0.05)；Dycal组TNF-α阳性细胞率明显高于正常牙髓(P<0.01)。
2盖髓后30天,黄芩苷海藻酸钠凝胶复合MTA组TNF-α阳性细胞率明显低于牙髓炎、Dycal组和MTA组(P<0.05)；MTA组TNF-α阳性细胞率高于正常牙髓(P<0.01),低于牙髓炎、Dycal组(P<0.05)；Dycal组TNF-α阳性细胞率高于正常牙髓(P<0.01),低于牙髓炎(P<0.01)。
结论
1 LPS法诱导犬实验性局限性牙髓炎简便可行,并能很好控制牙髓炎症程度。
2黄芩苷海藻酸钠凝胶复合MTA用于牙髓炎直接盖髓术,与氢氧化钙(Dycal)、MTA相比,TNF-a阳性细胞率减少,结果提示黄芩苷复合MTA可作为治疗牙髓炎的方法之一。
Objective
The purpose of this study was to establish an experimental model of beagle dogs pulpitis induced by lipopolysaccharide (LPS).The exposed pulps were capped with three different agents: baicalin sodium alginate gel combined with mineral trioxide aggregate (MTA), Dycal,MTA.The therapeutic effects of direct capping were assessed through immunohistochemistry at different time point. The result of this study will provide experimental evidences and theoretical basises for using baicalin sodium alginate gel combined with MTA to cure pulpitis.
Methods
Two healthy, SPF level beagle dogs(age:12～14 months) were randomly divided into two groups,which are 7 days group and 30 days group.Each beagle dog was established an experimental model of pulpitis induced by LPS. A therapy of pulp capping will be processed with baicalin sodium alginate gel combined with MTA. Dycal,MTA were used as pulp capping agents in control groups.The dogs were sacrificed at 7th day and 30th day after arteria carotis perfusion fixation. Taking out the experimental teeth to make the paraffin and thinest slices. Immunohistochemical stains were performed to identify the change of tumor necrosis factor-a(TNF-a)expression before and after pulp capping.
Results
1 At 7th day after pulp capping, the rate of TNF-a positive cells in baicalin sodium alginate gel combined with MTA group was lower than those in pulpitis, Dycal group and MTA group(P<0.05),higher than those in normal pulp(P<0.01).The rate of TNF-a positive cells in MTA group was lower than those in pulpitis (P<0.05).The rate of TNF-a positive cells in Dycal group was obviously higher than those in normal pulp(P<0.01).
2 At 30th day after pulp capping, the rate of TNF-a positive cells in baicalin sodium alginate gel combined with MTA group was lower than those in pulpitis, Dycal group and MTA group(P<0.05). The rate of TNF-a positive cells in MTA group was higher than those in normal pulp (P＜0.01), lower than those in pulpitis, Dycal group (P<0.05).The rate of TNF-a positive cells in Dycal group was higher than those in normal pulp (P<0.01), lower than those in pulpitis (P<0.01).
Conclusions
1 The method to establish an limited experimental model of beagle dog pulpitis induced by LPS was feasible.
2 It had better effects using baicalin sodium alginate gel combined with MTA as pulp capping agents in pulptis than Dycal or MTA, with having advantage of reducing the expression of TNF-a.
引文
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