逆针灸关元穴对去卵巢大鼠HPO轴的影响
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摘要
目的：
逆针灸是指疾病发生前或机体无病时,预先用针灸的方法激发经络之气,扶助正气,提高机体抵御疾病的能力,从而预防疾病的发生、减轻疾病的损害,达到保健延年的目的,是一种注重机体自身潜能的激发与利用的方法。逆针灸对下丘脑-垂体-卵巢组成的生殖功能调节轴(HPO)出现的功能失调有较好的预防作用。
围绝经期综合征是女性卵巢功能逐渐衰退直至完全消失的过程,由于这一过程中卵巢功能退行性的改变,卵巢停止排卵,雌激素缺乏,出现内分泌、代谢、神经、心理等各个系统失衡而引起的一组症候群。临床表现为月经的改变、潮热、汗出、手足心热、倦怠、易怒、头痛头晕、耳鸣、心悸、健忘、失眠等。西医通常应用雌激素替代疗法,但会引发各种副作用。于是,寻求有效并且安全的疗法逐渐成为研究热点。针灸治疗围绝经期综合征有确切的疗效,没有副作用,受到各研究人员的关注。但是临床及实验均以治疗为主,较少有报道预防为主的研究,且选穴缺少依据和客观标准
本实验通过观察逆针灸关元穴对随后去卵巢大鼠的影响,以探讨逆针灸调节HPO轴的具体机制。
方法：
实验动物分为空白组,假手术组,去卵巢组,逆针去卵巢组,逆灸去卵巢组。用酶联免疫法测大鼠的下丘脑、垂体、子宫及血清中的GnRH、FSH、LH E2、ER的水平变化,进一步探讨逆针灸防治围绝经期综合征过程中逆针和逆灸调节HPO轴的具体机制,来阐释针灸预防治疗围绝经期女性内分泌失调的作用机理,为临床治疗围绝经期综合征选穴提供实验依据。
结果：
1与正常组比较,模型组大鼠的组织和血清中E2、ER和GnRH水平显著降低(p<0.01), FSH和LH的水平显著升高(p<0.01)。
2与模型组比较,逆针和逆灸组的组织和血清中的E2、ER和GnRH水平均有不同程度地升高(p<0.05); FSH和LH水平有不同程度地降低(p<0.05)。
3逆针组和逆灸组比较,组间无显著差异(p>0.05)；但对血清FSH的调节,逆针组和逆灸组组间有显著差异,逆针效果优于逆灸(p<0.01)。
结论：
1逆针和逆灸可有效调节去卵巢大鼠低下的雌激素水平,并抑制增高的促性腺激素水平,能良性地调节去卵巢大鼠HPO轴的紊乱。
2逆针和逆灸关元穴对HPO轴的作用强度基本相同,没有显著差异。但是血清中FSH方面,逆针的效果优于逆灸。
3围绝经期综合征的产生和发展与下丘脑-垂体-卵巢轴密切相关,涉及到肝、脾、肾多个脏腑。因此从本实验的研究结果证实,治疗女性的围绝经期综合征应从多经脉和脏腑考虑,重视先天和后天的共同调理,并注重交会穴的使用。
Objective:
'Preventive acupuncture and moxibustion'mean to stimulate some points of the body by acupuncture or moxibustion in advance, which can improve the capability of defending diseases and supplementing'Zheng Qi'. And make the body have the function of preventing and alleviating the injury by the subsequent disease.'Preventive acupuncture and moxibustion'have better preventive function to the disorder of HPO axis.
Peri-menopausal syndrome (PMS) is a series of syndromes, which is caused by the ovarian failure and disorder of the endocrine system, external metabolizing system, nervous system and psychic system of women before and after menopause. Clinical manifestations are menstrual disorder, hectic fever with sweating, burning sensation of hands and feet, fatigue, testiness, headache, vertigo, sonitus, cardiopalmus, forgetfulness, insomnia, and so on. To western medicine, the application of hormone replacement therapy is the first choice for PMS, however, it has all kinds of side effects. So, to find a safe and effective treatment increasingly become the research highlight. As an effective therapy for PMS without any side effect, Acupuncture attracts much attention. But there is less report about how to prevent PMS. And the application of acupoints is in shortage of evidence and objective criteria.
By observing the regulatory effects of the preventive acupuncture and moxibustion at Guanyuan(RN4) points in ovariectomized rats. The research purpose of this study was to discuss the mechanism of'preventive acupuncture and moxibustion'on HPO axis.
Methods:
Experimental feminine SD rats were divided into5groups, including the normal group, fake operation group, ovariectomized rats group, preventive acupuncture group and preventive moxibustion group. ELISA was used to determine the levels of E2> ER、GnRH、FSH and LH in the serum and tissues including hypophysis、hypothalamus and uterus. The purpose of this study was to investigate the specificity of acupoints, estimate the regulatory effects of 'preventive acupuncture and moxibustion'on HPO axis, and also provide research evidences for acupoints selection and application in clinical practice.
Results:
1In comparison with the normal group, E2、ER and GnRH level in the tissue and serum of the model group was significantly lower(p<0.01), the levels of FSH and LH increased obviously(p<0.01).
2Compared with the model group, E2、ER and GnRH level of the'preventive acupuncture'group and'preventive moxibustion'group increased in different degree (p<0.05); FSH and LH level reduced in different degree (p<0.05)
3There is no obvious difference between the'preventive acupuncture'and 'preventive moxibustion'groups (p>0.05), except FSH of the blood,'preventive acupuncture'group is better than 'preventive moxibustion'group (p<0.01).
Conclusions:
1The'preventive acupuncture'and'preventive moxibustion'could modulate the lower estrogen level, and restrain elevated gonadotropin levels in ovariectomized rats, and has benign modulation to disordered HPO axis.
2The ability of the'preventive acupuncture'and'preventive moxibustion' are almost the same, except FSH of the blood,'preventive acupuncture'group is better than'preventive moxibustion'group.
3The origination and development of PMS is closely involved with the HPO axis, and relates to multiple organs, such as the liver, spleen and kidney. The results of this study suggest that meridians, multiple organs, and both innate and acquired functions should be considered in clinical acupoints election. And we should put the emphasis on the crossing acupoints for prevention and treatment of PMS.
逆针灸是指疾病发生前或机体无病时,预先用针灸的方法激发经络之气,扶助正气,提高机体抵御疾病的能力,从而预防疾病的发生、减轻疾病的损害,达到保健延年的目的,是一种注重机体自身潜能的激发与利用的方法。逆针灸对下丘脑-垂体-卵巢组成的生殖功能调节轴(HPO)出现的功能失调有较好的预防作用。
围绝经期综合征是女性卵巢功能逐渐衰退直至完全消失的过程,由于这一过程中卵巢功能退行性的改变,卵巢停止排卵,雌激素缺乏,出现内分泌、代谢、神经、心理等各个系统失衡而引起的一组症候群。临床表现为月经的改变、潮热、汗出、手足心热、倦怠、易怒、头痛头晕、耳鸣、心悸、健忘、失眠等。西医通常应用雌激素替代疗法,但会引发各种副作用。于是,寻求有效并且安全的疗法逐渐成为研究热点。针灸治疗围绝经期综合征有确切的疗效,没有副作用,受到各研究人员的关注。但是临床及实验均以治疗为主,较少有报道预防为主的研究,且选穴缺少依据和客观标准
本实验通过观察逆针灸关元穴对随后去卵巢大鼠的影响,以探讨逆针灸调节HPO轴的具体机制。
方法：
实验动物分为空白组,假手术组,去卵巢组,逆针去卵巢组,逆灸去卵巢组。用酶联免疫法测大鼠的下丘脑、垂体、子宫及血清中的GnRH、FSH、LH E2、ER的水平变化,进一步探讨逆针灸防治围绝经期综合征过程中逆针和逆灸调节HPO轴的具体机制,来阐释针灸预防治疗围绝经期女性内分泌失调的作用机理,为临床治疗围绝经期综合征选穴提供实验依据。
结果：
1与正常组比较,模型组大鼠的组织和血清中E2、ER和GnRH水平显著降低(p<0.01), FSH和LH的水平显著升高(p<0.01)。
2与模型组比较,逆针和逆灸组的组织和血清中的E2、ER和GnRH水平均有不同程度地升高(p<0.05); FSH和LH水平有不同程度地降低(p<0.05)。
3逆针组和逆灸组比较,组间无显著差异(p>0.05)；但对血清FSH的调节,逆针组和逆灸组组间有显著差异,逆针效果优于逆灸(p<0.01)。
结论：
1逆针和逆灸可有效调节去卵巢大鼠低下的雌激素水平,并抑制增高的促性腺激素水平,能良性地调节去卵巢大鼠HPO轴的紊乱。
2逆针和逆灸关元穴对HPO轴的作用强度基本相同,没有显著差异。但是血清中FSH方面,逆针的效果优于逆灸。
3围绝经期综合征的产生和发展与下丘脑-垂体-卵巢轴密切相关,涉及到肝、脾、肾多个脏腑。因此从本实验的研究结果证实,治疗女性的围绝经期综合征应从多经脉和脏腑考虑,重视先天和后天的共同调理,并注重交会穴的使用。
Objective:
'Preventive acupuncture and moxibustion'mean to stimulate some points of the body by acupuncture or moxibustion in advance, which can improve the capability of defending diseases and supplementing'Zheng Qi'. And make the body have the function of preventing and alleviating the injury by the subsequent disease.'Preventive acupuncture and moxibustion'have better preventive function to the disorder of HPO axis.
Peri-menopausal syndrome (PMS) is a series of syndromes, which is caused by the ovarian failure and disorder of the endocrine system, external metabolizing system, nervous system and psychic system of women before and after menopause. Clinical manifestations are menstrual disorder, hectic fever with sweating, burning sensation of hands and feet, fatigue, testiness, headache, vertigo, sonitus, cardiopalmus, forgetfulness, insomnia, and so on. To western medicine, the application of hormone replacement therapy is the first choice for PMS, however, it has all kinds of side effects. So, to find a safe and effective treatment increasingly become the research highlight. As an effective therapy for PMS without any side effect, Acupuncture attracts much attention. But there is less report about how to prevent PMS. And the application of acupoints is in shortage of evidence and objective criteria.
By observing the regulatory effects of the preventive acupuncture and moxibustion at Guanyuan(RN4) points in ovariectomized rats. The research purpose of this study was to discuss the mechanism of'preventive acupuncture and moxibustion'on HPO axis.
Methods:
Experimental feminine SD rats were divided into5groups, including the normal group, fake operation group, ovariectomized rats group, preventive acupuncture group and preventive moxibustion group. ELISA was used to determine the levels of E2> ER、GnRH、FSH and LH in the serum and tissues including hypophysis、hypothalamus and uterus. The purpose of this study was to investigate the specificity of acupoints, estimate the regulatory effects of 'preventive acupuncture and moxibustion'on HPO axis, and also provide research evidences for acupoints selection and application in clinical practice.
Results:
1In comparison with the normal group, E2、ER and GnRH level in the tissue and serum of the model group was significantly lower(p<0.01), the levels of FSH and LH increased obviously(p<0.01).
2Compared with the model group, E2、ER and GnRH level of the'preventive acupuncture'group and'preventive moxibustion'group increased in different degree (p<0.05); FSH and LH level reduced in different degree (p<0.05)
3There is no obvious difference between the'preventive acupuncture'and 'preventive moxibustion'groups (p>0.05), except FSH of the blood,'preventive acupuncture'group is better than 'preventive moxibustion'group (p<0.01).
Conclusions:
1The'preventive acupuncture'and'preventive moxibustion'could modulate the lower estrogen level, and restrain elevated gonadotropin levels in ovariectomized rats, and has benign modulation to disordered HPO axis.
2The ability of the'preventive acupuncture'and'preventive moxibustion' are almost the same, except FSH of the blood,'preventive acupuncture'group is better than'preventive moxibustion'group.
3The origination and development of PMS is closely involved with the HPO axis, and relates to multiple organs, such as the liver, spleen and kidney. The results of this study suggest that meridians, multiple organs, and both innate and acquired functions should be considered in clinical acupoints election. And we should put the emphasis on the crossing acupoints for prevention and treatment of PMS.
引文
[1]刘惠喜.妇产科学[M].北京医科大学出版社,2001,238-240.
[2]Lindh-Astrand L,Brynhildsen J, Hoffmann M, et al. Attitudes towards the menopause and hormone therapy over the turn of the century[J]. Maturitas,2007 56 (1):12220
[3]刘宏洋,孙远征.针灸治疗围绝经期综合征的概况[J].针灸临床杂志,2008,24(1)：50-51.
[4]乐杰主编.妇产科学(第六版)[M].人民卫生出版社,2004,5：349-350
[5]周云晓.性激素对女性膀胱出口梗阻影响的研究[D].浙江大学博士学位 论文2007
[6]郭伟男,孙立敏.围绝经期和绝经后子宫内膜息肉局部雌孕激素受体的表达及意义[J].河北医药,2011,33(21)：3218-3220
[7]鲁云钢,等.围绝经期功能失调性子宫出血的治疗[J].中外医疗2009,6：23
[8]吕润虎,等.女性更年期综合征的中西医认识[J].现代中西医结合杂志,2003,12(13)：1443
[9]FREEDMAN RR, NORTON D, WOODWARD S, e t a.1 Core body temperature and circadian rhythm o f hot flashes in menopausal women[J]. J Clin Endocrinol Metab,1995,80(8):2354-2358.
[10]GRIFFIN JD, KAPLEML, CHOW AR, etal Celluar mechanisms for neuronal thermo sensitivity in the rat hypothalamus[J]. J Physiol,1996,492:231-242
[11]FREEDMAN RR, KRELL W. Reduced thermo regulatory null zone in postmenopausal women with hot flushes [J].Am J Obstet Gynecol,1999,181(1): 66-70.
[12]王滨,刘宏艳,等.更年乐对围绝经期综合征网络机制影响的实验研究[J].江苏中医药,2003,23(10)：57
[13]张雅萍,等.坤宁安丸对更年期综合征患者生殖内分泌免疫功能的影响[J].中医药信息,2001,18(3)：52-54.
[14]桂勇,龚逞英,等.雌-孕激素与降脂药治疗围绝经期妇女高胆固醇血症疗效对比[J].实用临床医学,2010,11(2)：77-78
[15]郇玉红,孟江涛.血管回声跟踪技术评价围绝经期妇女颈总动脉弹性[J]新乡医学院学报,2008,25(4)：381-383
[16]杨建华.绝经后骨质疏松症及防治[J].中外医疗,2010,2：190
[17]郭俊仙.女性围绝经期血清雌激素与前臂骨密度随年龄变化的分析[J].中国妇幼保健,2011,26(17)：2633-2634
[18]张人尹.围绝经期妇女的肥胖证型分析[D].广州中医药大学硕士学位论文,2009
[19]Wing R R, Matthews K A, Kuller L H, et al. Weight gain at the time of menopause. Arch Med,1991,151:97-102.
[20]Laurey R, Simkin S, Rena R, et al. Weight gain during menopause. Postgraduate Medicine,2000,108(3)47-55
[21]于学文,高成阁,金辉.雌激素的抗抑郁作用[J].中华妇产科杂志,2003,38(2)：1132114.
[22]于学文,高成阁,等.雌激素对妇女围绝经与绝经期抑郁障碍的影响[J].中国妇幼健康研究,2007,18(5)：371-373
[23]高晓玲,王哲蔚,华嘉增,等.更年期妇女抑郁症状的发生情况及其影响因素的研究[J].中国妇幼保健杂志,1998,13(5)：294-296.
[24]王莉,王雅楠.更年期妇女心理健康状况及其相关因素[J].中国老年学杂志,2011,5(31)：1635-1636
[25]胡佳佳,周伯荣,等.女性围绝经期抑郁症与雌激素受体β基因多态性的相关性研究[J].安徽医科大学学报,2011,46(6)：562-565
[26]胡曼娜.围绝经期抑郁症与雌激素水平的相关性研究[D].新疆医科大学硕士学位论文,2008
[27]王洪图.皇帝内经素问白话解[M].人民卫生出版社,2010,4-5
[28]曾倩.杨家林生殖轴理论思想析义[J].辽宁中医杂志,2007,34(11)：15161517
[29]施茵,冯慧钧,刘慧荣.多囊卵巢综合征的发病机制及针灸治疗述评[J]针灸临床杂志,2008,24(7)：55-58
[30]李灿东,高碧珍,黄熙理等.不孕症肝郁病理与性腺轴功能变化的相关性研究[J].上海中医药大学学报,2005,19(3)：36-38
[31]胡凤媛.肾虚与卵巢早衰机理及补肾添精辨治述要[J].国医论坛,2007,22(4)：11-12
[32]肖炜.中药对去势大鼠神经内分泌免疫网络的影响[J].浙江中医药大学学报,2008,32(4)：443.
[33]张奇芳,姜恩魁,齐艳红.侧脑室注射芹菜素对动情期雌性大鼠生殖轴影响的研究[J].锦州医学院学报,2006,27(1)：41-43
[34]Danuta Wrona et al. Neural-immune interaction.Journal of Neuroimmunology 172(2006)38-58
[35]武骏,徐立,马红.宁心红杞胶囊对雌性老龄大鼠垂体-卵巢轴功能的影响[J].南京中医药大学学报,2007,23(1)：33-35
[36]Aimaretti G, Ambrosio MR, Di Somma C, et al. Traumati brain injury and subarachnoid haemorrhage are conditions a high risk for hypopituitarism: screening study at 3 months after the brain injury. Clinical Endocrinology,2004, 61 (3):320-326.
[37]BondanelliM, De Marinis L, Ambrosio MR, et al. Occurrence of pituitary dysfunction following traumatic brain injury.J Neurotrauma,2004,21 (6): 685-696
[38]张朝晖.电针对下丘脑-垂体-性腺轴的调节[J].国外医学计划生育／生殖健康分册,2007,26(1)：14-17
[39]韩冰,夏天,高慧等.冲任学说治疗卵巢功能失调性疾病[J].天津中医药大学学报.2008,27(3)：168-170
[40]宫静.电针不同穴位对去卵巢大鼠HPO轴影响的实验研究[D].北京中医药大学硕士学位论文.2010
[41]赵向东,史雪茹,施洪等.134例更年期妇女血清促卵泡激素、促黄体激素、雌二醇变化的相关性研究[J].实用诊断与治疗杂志,2004,18(2)：87-89
[42]周金山,晋志高,陶之理.关元一级感觉神经元在脊神经节的节段分布[J].上海针灸杂志,2001,20(3)：40-41.
[43]周金山,晋志高,陶之理.三阴交穴及子宫一级感觉神经元在脊神经节的节段分布(经穴相关的机理研究)[J].美国综合医学杂志,2000,2(6)：47.
[44]颜培宇.不同穴位针刺对免疫抑制大鼠神经肽相关基因表达及免疫调节作用的分子机制研究[D].黑龙江中医药大学博士学位论文,2010
[45]邹福清,赵海燕等.艾灸对急性髓系白血病化学治疗毒副反应的影响[J].河北中医,2011,33(8)：1201-1202
[46]江红柯.艾灸对运动训练大鼠糖原、血乳酸及运动能力的影响[J].河南大学学报(医学版),2011,30(4)：287-289
[47]恩和图娅.艾灸关元对化疗期生命体征影响的临床及实验研究[D].广州中医药大学博士学位论文,2010.
[48]王晓源,周忠光,郭迎喜.针刺“足三里”、“关元”穴对致衰小鼠睾丸组织抗氧化影响的实验研究[J].中医药信息,2010,27(2)：76-77
[49]河南焦作市第二人民医院针灸理疗科.针刺加TDP治疗慢性盆腔炎疗效观察[J].中国杜区医师·医学专业半月刊,2009,212(11)
[50]袁云霞.针刺治疗功能性痛经68例[J].上海针灸,2011,30(10)：698
[51]吴中朝,王玲玲.艾灸对老年人血浆睾酮、雌二醇的影响[J].中国针灸,1996,16(8)：27-28
[52]李晓泓,宋晓琳,张露芬,等.逆灸关元穴对自然更年期大鼠子宫HSP70,HSP70mRNA, SOD, NOS的影响[J].中华中医药杂志,2010,25(1)：46-49
[53]江瑜.艾灸关元、神阙穴对中期妊娠引产的疗效观察[J].中国中医基础医学杂志,2011,17(9)：1012-1013
[54]刘承,张海燕.艾灸治疗寒湿凝滞型原发性痛经疗效观察[J].世界针灸杂志,2011,21(3)：1-4
[55]张晓明,陈邦国.电针关元穴对脊髓不完全损伤大鼠尿动力学的影响[J]. 湖北中医学院学报,2009,11(3)：9-11
[56]高寅秋.电针促进腰麻后膀胱功能恢复疗效观察[D].中国中医科学院临床医学专业硕士学位论文,2011
[57]王丽芬,冯卫星.针刺配合葱白盐炒热敷治疗脑血管病后尿储留30例[J].2007,28(10)：1385
[58]尚艳杰,崔晓梅.关元、中极穴治疗慢性非细菌性前列腺炎的临床观察[J].黑龙江中医药,2008,2：39-40
[59]秦东霞.针刺治疗小儿神经性尿频42例[J].长春中医药大学学报2010,26(3)：411-412
[60]于竹力.针刺足运感区结合温针关元、中极治疗小儿遗尿[J].针灸临床杂志,2011,27(10)：16-17
[61]李改凤.针刺配合足底按摩治疗女性尿失禁40例[J].陕西中医,2007,28(3)：329
[62]郑燕生,赵铭峰,王伟.薄氏腹针对粘连性肠梗阻患者的止痛效果观察[C].针灸治疗痛证国际学术研讨会论文汇编2009,104-107
[63]邢金云.腹针防治含顺铂方案化疗所致恶心、呕吐的临床研究[D].北京中医药大学中医学专业硕士学位论文2011
[64]于磊.艾灸治疗慢性结肠炎20例[C].中国针灸协会针推结合专业委员会成立大会暨针灸教育与腧穴应用学术研讨会论文汇编,2010：341
[65]蔡焦生,杨树明,张爱萍.浅析艾灸关元穴治疗恶性腹水的机制[J].中国医药指南,2008,24(6)：314-315
[66]朱微微.针灸治疗厄逆50例分析[J].安徽卫生职业技术学院学报,2007,6(6)：33
[67]唐泗明,黄安清.艾灸关元穴治疗直肠脱垂57例分析[J].山西中医,2009,25(1)：25
[68]周金黄等.衰老、抗衰老、老年医学[M].北京：中国科学技术出版社,1993：54,160.
[69]伦新,荣莉.足三里、关元隔姜灸对老年人SOD,T细胞亚群的影响[J].新中医,2000,(4)：24.
[70]刘斌,王业秋等.针灸“足三里”及“关元”对大鼠皮肤光老化的防护作用[J].针灸临床杂志,2010,26(8)：56-57
[71]刘莹莹,等.艾灸对老年患者抗衰老的临床研究[J].河北医学,2009,15(4)：431-433
[72]马力佳,等.针灸对老年患者P16基因定量表达的影响[D].南京中医药 大学硕士学位论文,2010
[73]陈仲新.针刺配合饮食控制治疗单纯性肥胖疗效观察[J].中国针灸,2008,28(12)：888-890
[74]张梅.穴位埋线治疗更年期妇女肥胖的临床研究及其对血清生殖激素的影响[D].广州中医药大学学位论文,2008
[75]何立,等.针刺对单纯性肥胖症体重指数及腰臀围比的影响[J].中国针灸,2008,28(2)：95-97
[76]赵霞.针刺配合耳穴贴压治疗单纯性肥胖症的临床观察[D].黑龙江中医药大学硕士学位论文.2007
[77]贾燕亲,马文珠,赵静洁,李京真,陈彦安.艾灸减肥机制初探[C].2011中国针灸学会年会论文集,2011：729-733
[78]葛建军,王少锦,等.不同频率电针刺激对实验性肥胖大鼠减肥效应的影响[J].中国针灸,2007,27(8)：598-600
[79]黄文婷等.腹针对脑缺血再灌注大鼠运动功能和Nogo-A的影响[D].广州中医药大学硕士学位论文2011
[80]黄嫚,朱雄.腹针为主治疗肌张力低下型脑瘫验案二则[C].2011中国针灸协会年会论文集,2011
[81]孙景波,黄文婷.腹针引气归元法治疗中风病机制研究的思路与方法[C].
2011中国针灸协会年会论文集,2011：181-184
[82]陈真悟.腹针与体针治疗中风后抑郁症的疗效对比研究[D].广州中医药大学硕士学位论文2010
[83]陈晓军,方针.针刺“不寐四穴”为主治疗不寐21例[J].中国针灸,2009,29(4)：278
[84]汤耿民.腹部针刺法治疗慢性风湿性关节炎36例临床报告[J]世界中医骨科杂志,2010,11(1)：13-18
[85]张苗苗,孟凡迅等.大鼠任脉、督脉不同腧穴的针刺痛域相关性比较[J]四川中医,2007,25(6)：14-15
[1]王洪图主编《皇帝内经·素问·刺疟篇第三十六》[M]人民卫生出版社2006：248
[2]张慧.逆针对自然更年期大鼠生殖内分泌及免疫功能影响的实验研究[D]. 北京中医药大学硕士研究生学位论文,2005
[3]陈昌钧.兽医激光手术前麻醉[J].四川畜牧兽医,1980,2
[4]郑玲.“逆针灸”对更年期大鼠热休克蛋白及应激相关机制影响的研究[D].北京中医药大学硕士研究生学位论文,2007
[5]李晓泓,宋晓琳,徐莉莉,等.不同介入时机针刺三阴交穴对痛经大鼠下丘脑、垂体β-EP含量和HSP70表达的影响研究[J].中华中医药杂志,2010,25(9)：1456-1458
[6]刘丹,韩威,张迪.不同时间针刺对局灶性脑缺血再灌注大鼠BCL-2 Bax蛋白表达的影响[J].中华中医药学刊,2007,25(3)：622-623
[7]康世荚,熊星火,等.不同时间针刺对正常大鼠糖耐量的影响[J].上海针灸杂志,1996,15(4)：33-34
[8]叶飞,董军立,等.假性球麻痹后不同时间针刺风池穴的疗效比较[J].中国康复,2008,23(2)：88-89
[9]Selye H. Stress in health and disease. Boston:Butterworth,1976.18-45
[10]Laue L.etal.In:Chrousos GP, Lynn D&Gold PW. Eds. Mechanisms of Physical and Emotional Stress. New York and London:Pleuum press. 1988,225:235
[11]M izush ima Y, et al. Am J Physiol Regual In teger Comp Physiology,2000, 278(2):352-359.
[12]郭一闽.手术和术后镇痛对细胞因子的影响[J].临床医学,2008.28(4)：104-107
[13]何云云,何昆仑,刘春蕾.内质网应激与心血管疾病研究概述[J].生理科学进展,2011.42(6)：419-422
[14]B·M·舒贝克(苏)运动员身体负荷的免疫学研究[J].武汉体院译报,1987,4
[15]王茂叶.递增负荷运动对大鼠下丘脑一垂体一肾上腺皮质轴的影响研究[D].山东师范大学博士学位论文
[16]Laybutt DR, Preston AM, Akerfeldt MC, etal. Endoplasmic reticulum stress contritutes to beta cell apoptosis in type2 diabetes [J]. Diabetologia,2007, 50(4):752-763.
[17]Liu J, Jin X, Yu CH, etal. Endoplasmic reticulum stress involved in the course of lipogenesis in fatty acids-induced hepatic steatosis [J]. Gastroenterol Hepatol, 2010,25(3):613-618
[18]Fonseca SG, Burcin M, Gromada J, etal. Endoplasmic reticulum stress in beta cells and development of diabetes [J].Curr Opin Pharmacol,2009,9(6):763-770.
[19]Tabas I. The role of endoplasmic reticulum stress in the progression of atherosclerosis[J]. Circ Res,2010,107(7):839-850.
[20]劳宁.电针灸诱导脑缺血耐受作用的研究[D].第四军医大学学位论文2003
[21]周华,孙国杰等.针灸预处理对阿尔茨海默病大鼠学习记忆行为的影响[J].湖北中医药大学学报2011.13(1)：3-5
[22]Birnbaum Y, Hale SL Kloner RA. Ischemic preconditioning at a distance: reduction of myocardial infarctsize by partial reduction of blood suppy combined with rapid stimulation of the gastrocnemius muscle in the rabbit. Circulation 1997;96(5):1641-6
[23]李晓泓.针灸“治未病”与“针灸良性预应激假说”[J].北京中医药大学学报,2003,26(3)：82-85
[24]翟景慧,李晓泓,张露芬,等.“逆针”与“逆灸”对去卵巢大鼠血脂水平的影响[J].针刺研究,2005,30(2)：80-82
[25]张露芬,李晓泓,张慧,等.逆针关元穴对自然围绝经期大鼠血脂的调节作用[J].中国临床康复,2005,9(23)：132-133
[26]解秸萍,李晓泓,张露芬,等.“逆灸”调节大鼠围绝经期血脂、体重的效应规律[J].北京中医药大学学报,2006,29(1)：67-70
[27]李晓泓,解秸萍,张露芬,等.逆灸关元穴对自然围绝经期大鼠下丘脑雌激素受体α和促肾上腺激素释放激素及血浆促肾上腺激素的调节[J].中国临床康复,2006,10(7)：118-120
[28]段淑香,李亚东,李健男,等.针刺对围绝经期雌性大鼠血清雌二醇、睾酮、促性腺激素水平的影响[J].针灸临床杂志,2006,22(11)：44-45
[29]胡玲,汪惠丽,高忻洙,等.电针“关元”与“三阴交”对围绝经期模型大鼠性激素及下丘脑β-EP调整作用的比较[J].中国针灸,2004,24(9)：649-651
[30]张皓.针刺干预后围绝经期综合征患者临床症状及β一内啡肽的改变[J].国临床康复,2006,10(31)：1-3
[31]何玉伟,李晓泓.逆针灸对去卵巢大鼠生殖内分泌影响的研究[J].中华中医药学刊,2008,26(9)：1918-1920
[32]郑玲.“逆针灸”对更年期大鼠热休克蛋白及应激相关机制影响的研究[D].北京中医药大学硕士研究生学位论文
[33]李晓泓,郑玲.“逆针灸”干预对去卵巢大鼠下丘脑不同核团ER-α和ER-a mRNA表达水平的影响[J].中国针灸,2008,28(12)：918-921
[34]李晓泓,郑玲.逆针灸对去卵巢大鼠子宫保护效应的观察[J].针刺研究,2008,33(4)：229-234
[35]莫捷,李晓泓.逆针灸关元穴对12月龄自然更年期大鼠体质量增长率、下丘脑和卵巢瘦素含量的影响[J].天津中医药,2010,27(5)：383-385
[36]李晓泓,莫捷.“逆灸”关元穴对更年期大鼠体重增长率血脂和腹部脂肪胰岛素受体表达的影响[C].中国针灸学会临床分会第十七届全国针灸临床学术研讨会论文集,2009
[37]曹文忠,张莉,宋书邦,等.针灸预防中风后遗症复发的临床研究[J].中国针灸,2003,23(6)：317-320
[38]高俊虹,付卫星,晋志高,等.β-肾上腺素受体后信号转导通路介导电针预治疗抗缺血性心律失常的作用[J].中国针灸,2006,26(6)：431-435
[39]田代实,邓医字,王光海,等.针刺预处理对大鼠局灶性脑缺血的抗细胞凋亡作用[J].第四军医大学学报,2004,25(1)：27
[40]李晓泓,张露芬,郭顺根,等.逆灸对随后佐剂性关节炎大鼠早期及继发期炎性细胞因子和局部足肿胀的影响[J].中国临床康复,2005,9(15)：23-125
[41]杨运宽,刁灿阳,胡幼平,等.针灸防治肝癌概况[J].中国针灸,2002,22(9)：638-641
[42]徐正海.逆灸预防绝经后骨质疏松症的临床观察[J].中国中医药信息杂志,2001,8(1):79
[43]秦明,黄裕新,王景杰,等.预防性针刺足三里穴对冷应激大鼠胃粘膜损伤的影响[J].西南国防医药,2005,15(2)：33-135
[44]齐素云,王亚莉.电针预防广泛子宫切除术后尿潴留[J].河北医学,2005,11(4)：379-380
[45]施琴,针刺足三里、内关穴预防化疗胃肠道反应疗效观察[J].中医药临床杂志,2004,16(5)481
[46]程金莲,王麟鹏.“王氏夹脊穴”逆针灸治疗对脑卒中偏瘫痉挛状态117例随机对照观察[J].北京中医,2007.26(8)：467-469
[47]王樟连,陈利芳.逆针灸对缺血性卒中一级预防的价值探微[J].中华中医药学刊,2009.27(9)：1834-1836
[48]王少松.逆针灸针刺“王氏夹脊穴”防治中风后肌张力增高的临床研究[D].北京中医药大学硕士学位论文,2007
[49]高俊虹,晋志高.电针预处理改善心肌缺血性损伤的β1—肾上腺素能受体信号转导机制[D].中国中医科学院2003级博士研究生学位论文,2003
[50]黄秀容,袁青.逆针灸预防运动员关节损伤应用研究[D].广州中医药大学 硕士学位论文,2010
[51]李虹霖,孙远征.头穴透刺对运动性疲劳大鼠部分生化指标及氨基酸的影响[J].上海针灸杂志,2009.28(12)：738-740
[52]崔丽,孙国杰,周华,杜艳君.针灸预刺激对阿尔茨海默病模型大鼠学习记忆能力及海马区SOD和NOS的影响[J].湖北中医学院学报,2009.11(3)：6-8
[53]豁银成.针灸预刺激对AD大鼠神经元凋亡相关蛋Bc1-2,NF-KB的影响[D].湖北中医药大学硕士学位论文,2010
[54]莫捷,李晓泓等.逆针灸关元穴对12月龄自然更年期大鼠体质量增长率、下丘脑和卵巢瘦素含量的影响[J].天津中医药,2010,27(5)：383-385
[1]Vander EM, Horvath TL, Wiegant VM, et al. Evidence for a direct neuronal pathway from the suprachiasmatic nucleus to the gonadotropin-releasing hormone system:combined tracing and light and electron microscopic immunocytochemical studies. J Comp Neurol,1997; 384(8):569
[2]Mallick HN, Kumar VM. Gating of the dorsal penile nerve inputs by norepinephrine at the medial preoptic area in rats. Indian J Physiol Pharmacol, 2005;49(3):325
[3]SilerK, Theresa M, Grayson M,et al. GnRH and its anologues bind the human placental receptor[J]. Journalof the Society for Gynecologic Investigation,2001, 8(4):233-238.
[4]Wang L, Xie L P, HuangW Q,et al. Presence of gonadotropin releasing hormone (GnRH) and its mRNA in rat pancreas[J]. Molecular and Cellular Endocrinology,2001,172(1-2):185-191
[5]Huang W Q, Yao B, Sun L,et al. Immunohistochemical and in situ hybridization studies of GnRH and its receptor [J]. Life Science,2001,68(15):1727-1734.
[6]Chen H F, Jeung E B, Stephenson M,etal. Human peripheral blood mononuclear cells express gonadotropin releasing hormone (GnRH), GnRH receptor, and interleukin-2 receptor gamma-chain messenger ribonucleic acids thatare regulated by GnRH in vitro[J]. Journal of Clinical Endocrinology and Metabolism,1999,84:743-750
[7]StanislausD, Pinter JH, JanovickJA, et al. Mechanisms mediating multiple physiological responses to gonadotropin-releasing hormone[J].Molecular and Cellular Endocronology,1998,114:1-10
[8]张萍.促性腺激素释放激素系统与妇科肿瘤的研究进展[J].上海第二医科大学学报,2005,125(5)：535-536
[9]Smith MJ, Jennes L. Neural signals that regulate GnRH neurones directly during the oestrous cycle. J Reproduction,2001; 122(1):1
[10]Irahara M, Tamura T, Matuzaki T, et al. Orexin suppresses the pulsatile secretion of luteinizing hormone via beta endorphin. J Biochem Biophys Res, 2001;281(1):232
[11]Vincent P. Glial-neuronal-endothelial interactions are involved in the control of GnRH secrection [J]. JNeuroendocrin,2002,14
[12]金晓航,黄威权.促性腺激素释放激素免疫调节作用的研究进展[J].生理科学进展,2000,31(2)：169-172
[13]徐苓.老年妇女内分泌变化[J].中国实用妇科与产科杂志,2002,18(12)：721
[14]葛秦生,孙亦彬,刘士廉,等.临床生殖内分泌学[M].北京：科学技术文献出版社,2001,186-192,386-401
[15]何淑莹,申玉华,尤昭玲.多囊卵巢综合症LH及FSH的研究现状[J].中医药导报,2005,11(8)：82-84
[16]刘春杰,纪凤兰,董立珉等.二仙汤对更年期肾阳虚大鼠的影响[J].长春中医学院学报,2006,22(1)：61-62
[17]王洪珍,陈霁,李彩红.绝经后妇女血脂代谢的变化及激素替代治疗的影响[J].江苏大学学报(医学版),2006,16(2)：151-153
[18]Bellipanni G, Bianchi P, Pierpaoli Wet al. Effects of melatonin in perimenopausal and menopausal women:a randomized and placebo controlled study. Exp Gerontol,2001,36(2):297
[19]肖炜,邓虹珠,马云.益妇宁软胶囊治疗更年期综合征的实验研究[J].中国中药杂志,2003,28(3)：253-257
[20]Garrido-Gracia JC, Bellido C, Aguilar R, et al. Protein kinase C cross-talk with gonadotrope progesterone receptor is involved in GnRH-induced LH secretion. J Physiol Biochem,2006,62(1):35-42
[21]Stearns V, Hayes D F. Approach to menopausal symptoms in women with breast cancer [J]. Curr Treat Options Oncol 2002,3 (2):179
[22]Yun-Jun Ruan, Jian Qiu, Sai-Zhu Wu, et al. Effects of estrogen and its receptor on the production of nitric oxide in vascular endothelial cells, Chinese Journal of Clinical Rehabilitation,2004,8(6):1150-1151
[23]Shaw L, Taggart MJ, Austin C. Mechanisms of 17 beta-oestradiol induced vasodilatation in isolated pressurized rat small arteries[J].Cr J Pharmacol,2000, 129:555-565
[24]Razandi M, PedramA, Levin ER. Estrogen signalsto the preservation of endothelial cell form and function [J]. J Biol Chem,2000,275:38540-38546
[25]Sudoh N, Toba K, Akishita M, et al. Estrogen prevent soxidative stress induced endothelial cell apoptosis in rats[J]. Circulation,2001,103:724-729
[26]Stein DG. Brain damage, sex hormones and recovery:a new role for progesterone and estrogen[J]. Trends Neurosc 2001,7:386
[27]Burger HG, Dudley E, Mamers P, eta.l Early follicular phase serum FSH as a function of age:the role of inhibin B, inhibin A and estradiol.Climacteric,2000; 3(1):17
[28]Vaninetti S, Baccarelli A, Romoli R, et al. Effect of aging on serum gonadotropin levels in healthy subjects and patients with nonfunctioning pituitary adenomas. Europ J Endocr,i 2000; 142:144 [29]Wang Q, Santizo R, Baughman VL, et a.l Estrogen provides neuro protection in transient forebrain ischemia through perfusion-independent mechanisms in rats [J]. Stroke,1999,30(3):630
[30]李露斯,王兴华,范文辉.雌二醇对慢性脑缺血去卵巢大鼠脑病理改变的影响[J].第三军医大学学报,2002,24(12)：1391-1393
[31]汪艳丽,季其仁,肖玲玲等.围绝经期、绝经后妇女性激素水平的变化及其与糖、脂代谢的关系[J].安徽医学,2005,26(1)：27-28
[32]PaulS. Cooke, Afia. Role of Estrogens in Adipocyte Development and Function[J]. Exp Biol Med,2004,229:1127-1135.
[33]Wagner JD, Thomas MJ, Williams JK, et al. Insulin sensitivity and cardiovascular risk factors in ovariectomized monkeys with estradiol alone or combined with nomegestrol acetate. J Clin Endocrinol Metab,1998,83(3):896.
[34]王方杰,王吉听.胰岛素抵抗与糖尿病、肥胖、冠心病、脂代谢异常的相关性[J].实用心脑肺血管病杂志,2005,13(1)：53-55
[35]Sribastava M, Deal C·Osteoporosis in elderly:prevention and treatment[J].Clin Geriatr Med 2002,18(3):529-555
[36]Li NH,Ou PZ,ZhuHM, et al·Comparasion study on risk factors of osteopenia in the middle-aged and elderly men women population[J]·Zhongguo Linchuang Kangfu(Chin J Clin Rehabil)2002,6(3):382-383·
[37]苏汝堃,刘兴漠,邓颖辉.骨质疏松患者472例X线表现及骨密度分析[J].中国临床康复,2004,8(2)：4355
[38]Vogelvang TE, vander Mooren MJ, Mijatovic V. Hormone replacement therapy, selective estrogen receptor modulators, and tissue-specific compounds: cardiovascular effects and clinical implications. Treat Endocrinol,2004,3:105-115
[39]amada M, Irahara M,Maegawa M,et al.Transient increase in thelevels of T-helper 1 cytokines in postmenopausal women and the effects of hormone replacement.Therapy Gynecol and Obstet Invest,2001,52:82
[40]Kanda N,Tamaki K.Estrogen enhances immunoglobulin production by human PBM CS.J Allergy Clin Immunol,1999,103:282
[41]Raikkonen K, Mattbews KA, Kuller LH. The relationship between psychological risk attributes and the metabolic syndrome in healthy women: antecedent or consequence? Metabolism,2002,51(12):1573-1577
[42]Moe KE. Reproductive hormones, aging and sleep. Semin Reprod Endocrinol, 1999,17 (4):339
[43]NetzerN C, Eliasson A H, StrohlK P.Women with sleep apnea have lower levels of sex hormones·Sleep Breath,2003,7 (1):25
[44]PoloK P, Erkkola R, Irjala K et al.Effect of short-term transdermal estrogen replacement therapy on sleep:a randomized, double-blind crossover trial in postmenopausal women. Fertil Steri,1 1999,71 (5):873
[45]Crawford SL, Johannes CB, Stellato RK. Assessment of digit preference in self-reported year at menopause:chioce of an appropriate reference distribution. Am J Epidemiol 2002,156(7):676-683
[46]吴小立,张晋碚,温盛霖,等.围绝经期情绪障碍与雌二醇相关性的探讨[J].中国神经精神疾病杂志,2005,31(6)：455-457
[47]Drapier-Faure E, Azoulay C, Abramovici Y. Acceptability of continuous combined versus cyclical HRT:a French multi-centric randomized clinical study [J]. Gynecol Obstet Fertil,2005,33(7-8):498-504
[48]冷雯,陈沿东,陈岚华.雌孕激素阻断替代治疗围绝经期综合征研究初探[J].江苏医药,2004,30(5)：390
[49]朱建华,郭开华,徐杰.染料木素对去卵巢大鼠学习记忆能力的作用[J].中 国临床康复,2004,8(4)：650-651
[50]鲁兴梅.对雌激素来源和受体及其作用的再认识[J].卫生职业教育,2004,22：152-154
[51]UZAKI S, FUKAYA T, SUZUKI T, et al. Oestrogen receptor alpha and beta mRNA expression in human endometrium throughout the menstrual cycle[J]. Mol Hum an Repord,1999,5 (6):559-564.
[52]LAU KM,LASP INA M,LONG J, et al. Expression of Est2 rogen receptor (ER) 2alpha and ER2beta in nomal and malignant prostatic epithelial cell regulation by methylation and involvement in growth regulation [J]. Cancer Res,2000,60 (12):3175-3182
[53]王红,张元珍,刘文惠.雌激素替代治疗对去势大鼠阴道组织雌激素受体表达的影响[J].医药导报,2008,27(11)：1304-1306
[54]张吉强,蔡文琴.脑内雌激素受体的研究进展[J].局解手术学杂志,2009,18(1).
[55]Simoncini T, Mannella p, Fornari L, et al. Genomic and nongenomic effects of estrogens on endothelial cells[J]. Steroids,2004,69(8-9):537-542
[56]张吉强,蔡文琴.雌激素受体研究进展[J].生理科学进展,2001,32(1)68-70.
[57]Nagata H, Nozaki M, Nakano H. Short-term combinational therapy of low-dose estrogen with selective serotonin re-uptake inhibitor(fluvoxamine) for oophorectomized women with hot flashes and depressive tendencies [J]. J Obstet Gynaecol Res,2005,31(2)
[58]李红,任林,李灿东,林雪娟.围绝经期综合征肝气郁结证患者雌激素受体β基因多态性分析[J].中华中医药杂志,2010,25(6)：830-832
[59]胡佳佳,周伯荣,秦媛怡,张慧敏。女性围绝经期抑郁症与雌激素受体β基因多态性的相关性研究[J].安徽医科大学学报,2011,46(6)：562-565
[60]Miehael R. Soules, Sherry Sherman, Estella P ott, et al. Stages of Reproduetive Aging OrkshoP(STRAW)[J].M men,5Health&Gender-based Medicine. 2001;10(9):843-849.
[61]Janssenl, Heymsfield SB, Allison DB, et al. Body mass index and waist circumference independently contribute to the prediction of non abdominal, abdominal subcutaneous, and visceral fat [J].Am J Clin Nutr,2002,75(4):683
[62]Henry G. Burger, EmmaC. Dudley, JiShengCui, et al. A prospective longitudinal study of serum testosterone, dehydroe Piandrosterone sulfate, and sex hormone binding globulin levels through the menopause transition[J].Clin Endocrinol Metab2000,85:2832-2838
[63]Takahiro mizutani,yoshinobu nishikawa,hiroshi adachi,et al.Identification of estrogen receptor in human adipose tissue and adipocytes.J Chin Endocrionl Metab,1991,78:950-954.
[64]Wade GN, Gray JM.Cytoplasmic 17-β[3H]estradiol binding in rat adipose tissues.Endocrinology,1978,103:1695-1701.
[65]Gray JM, Wade GN.Cytoplasmic progestin binding in rat adipose tissues.Endocrinology,1979,104:1377-1382.
[66]SB. Pedesen, PS. Hansen, S Lund, et al. Identification of estrogen receptors and estrogen recepter mRNA in human adipose tissue. Eur J Clin Invest,1996, 26:262-269.
[67]赵文燕,李英敏.脂肪细胞雌激素受体的测定及表达水平的研究[J].河北医药,2004,26(9)：716-717
[68]Homma H, Kurachi H, Nishio Y, et al. Estrogen sup2 p resses t ranscrip tion of lip op rotein lip ase gene [J]. J Bi2 ological Chemist ry,2000,275:11404-11411.
[69]Heine PA, Taylor JA, Lwamot o GA, et al. Increased adipose tissue in male and female estrogen receptor2aknockout mice [J]. Proc Natl Acad Sci USA,2000, 97 12729-12734.
[70]赵文燕,董耀,仇树林.脂肪细胞雌激素受体的表达及其与人体脂肪分布的相关性[J].中国美容医学,2005,14(5)：534-536
[71]朱晓晖,王军,印彤.雌激素干预影响腹腔脂肪积聚的实验研究[J].中国临床药理学与治疗学,2003,8(6)：669-672
[72]Edwin LB, John AG. Disorders of lipid metabolism[A]. In:Robert HW, ed. Textbook of Endocrinology[M]. Philadelphia:PA. W.B.Saunders,1992:1367
[73]Mattiasson I, Rendell M, Tomquist C, et al. Effects of estro-gen replacement therapy on abdominal fat compartments as related to glucose and lipid metabolism in early postmenopausal women[J]. HormMetab Res,2002;34:583-8
[74]Perrone G, LiuY, Capri O, et al. Evaluation of the body composition and fat distribution in long-term users of hormone replacement therapy. Gynecol Obstet Invest.1999,48(1):52-55.
[75]李琦.绝经后早期女性体脂分布的特点及其与雄激素水平和骨密度的相关 性[D].复旦大学硕士学位论文,2010
[2]Lindh-Astrand L,Brynhildsen J, Hoffmann M, et al. Attitudes towards the menopause and hormone therapy over the turn of the century[J]. Maturitas,2007 56 (1):12220
[3]刘宏洋,孙远征.针灸治疗围绝经期综合征的概况[J].针灸临床杂志,2008,24(1)：50-51.
[4]乐杰主编.妇产科学(第六版)[M].人民卫生出版社,2004,5：349-350
[5]周云晓.性激素对女性膀胱出口梗阻影响的研究[D].浙江大学博士学位 论文2007
[6]郭伟男,孙立敏.围绝经期和绝经后子宫内膜息肉局部雌孕激素受体的表达及意义[J].河北医药,2011,33(21)：3218-3220
[7]鲁云钢,等.围绝经期功能失调性子宫出血的治疗[J].中外医疗2009,6：23
[8]吕润虎,等.女性更年期综合征的中西医认识[J].现代中西医结合杂志,2003,12(13)：1443
[9]FREEDMAN RR, NORTON D, WOODWARD S, e t a.1 Core body temperature and circadian rhythm o f hot flashes in menopausal women[J]. J Clin Endocrinol Metab,1995,80(8):2354-2358.
[10]GRIFFIN JD, KAPLEML, CHOW AR, etal Celluar mechanisms for neuronal thermo sensitivity in the rat hypothalamus[J]. J Physiol,1996,492:231-242
[11]FREEDMAN RR, KRELL W. Reduced thermo regulatory null zone in postmenopausal women with hot flushes [J].Am J Obstet Gynecol,1999,181(1): 66-70.
[12]王滨,刘宏艳,等.更年乐对围绝经期综合征网络机制影响的实验研究[J].江苏中医药,2003,23(10)：57
[13]张雅萍,等.坤宁安丸对更年期综合征患者生殖内分泌免疫功能的影响[J].中医药信息,2001,18(3)：52-54.
[14]桂勇,龚逞英,等.雌-孕激素与降脂药治疗围绝经期妇女高胆固醇血症疗效对比[J].实用临床医学,2010,11(2)：77-78
[15]郇玉红,孟江涛.血管回声跟踪技术评价围绝经期妇女颈总动脉弹性[J]新乡医学院学报,2008,25(4)：381-383
[16]杨建华.绝经后骨质疏松症及防治[J].中外医疗,2010,2：190
[17]郭俊仙.女性围绝经期血清雌激素与前臂骨密度随年龄变化的分析[J].中国妇幼保健,2011,26(17)：2633-2634
[18]张人尹.围绝经期妇女的肥胖证型分析[D].广州中医药大学硕士学位论文,2009
[19]Wing R R, Matthews K A, Kuller L H, et al. Weight gain at the time of menopause. Arch Med,1991,151:97-102.
[20]Laurey R, Simkin S, Rena R, et al. Weight gain during menopause. Postgraduate Medicine,2000,108(3)47-55
[21]于学文,高成阁,金辉.雌激素的抗抑郁作用[J].中华妇产科杂志,2003,38(2)：1132114.
[22]于学文,高成阁,等.雌激素对妇女围绝经与绝经期抑郁障碍的影响[J].中国妇幼健康研究,2007,18(5)：371-373
[23]高晓玲,王哲蔚,华嘉增,等.更年期妇女抑郁症状的发生情况及其影响因素的研究[J].中国妇幼保健杂志,1998,13(5)：294-296.
[24]王莉,王雅楠.更年期妇女心理健康状况及其相关因素[J].中国老年学杂志,2011,5(31)：1635-1636
[25]胡佳佳,周伯荣,等.女性围绝经期抑郁症与雌激素受体β基因多态性的相关性研究[J].安徽医科大学学报,2011,46(6)：562-565
[26]胡曼娜.围绝经期抑郁症与雌激素水平的相关性研究[D].新疆医科大学硕士学位论文,2008
[27]王洪图.皇帝内经素问白话解[M].人民卫生出版社,2010,4-5
[28]曾倩.杨家林生殖轴理论思想析义[J].辽宁中医杂志,2007,34(11)：15161517
[29]施茵,冯慧钧,刘慧荣.多囊卵巢综合征的发病机制及针灸治疗述评[J]针灸临床杂志,2008,24(7)：55-58
[30]李灿东,高碧珍,黄熙理等.不孕症肝郁病理与性腺轴功能变化的相关性研究[J].上海中医药大学学报,2005,19(3)：36-38
[31]胡凤媛.肾虚与卵巢早衰机理及补肾添精辨治述要[J].国医论坛,2007,22(4)：11-12
[32]肖炜.中药对去势大鼠神经内分泌免疫网络的影响[J].浙江中医药大学学报,2008,32(4)：443.
[33]张奇芳,姜恩魁,齐艳红.侧脑室注射芹菜素对动情期雌性大鼠生殖轴影响的研究[J].锦州医学院学报,2006,27(1)：41-43
[34]Danuta Wrona et al. Neural-immune interaction.Journal of Neuroimmunology 172(2006)38-58
[35]武骏,徐立,马红.宁心红杞胶囊对雌性老龄大鼠垂体-卵巢轴功能的影响[J].南京中医药大学学报,2007,23(1)：33-35
[36]Aimaretti G, Ambrosio MR, Di Somma C, et al. Traumati brain injury and subarachnoid haemorrhage are conditions a high risk for hypopituitarism: screening study at 3 months after the brain injury. Clinical Endocrinology,2004, 61 (3):320-326.
[37]BondanelliM, De Marinis L, Ambrosio MR, et al. Occurrence of pituitary dysfunction following traumatic brain injury.J Neurotrauma,2004,21 (6): 685-696
[38]张朝晖.电针对下丘脑-垂体-性腺轴的调节[J].国外医学计划生育／生殖健康分册,2007,26(1)：14-17
[39]韩冰,夏天,高慧等.冲任学说治疗卵巢功能失调性疾病[J].天津中医药大学学报.2008,27(3)：168-170
[40]宫静.电针不同穴位对去卵巢大鼠HPO轴影响的实验研究[D].北京中医药大学硕士学位论文.2010
[41]赵向东,史雪茹,施洪等.134例更年期妇女血清促卵泡激素、促黄体激素、雌二醇变化的相关性研究[J].实用诊断与治疗杂志,2004,18(2)：87-89
[42]周金山,晋志高,陶之理.关元一级感觉神经元在脊神经节的节段分布[J].上海针灸杂志,2001,20(3)：40-41.
[43]周金山,晋志高,陶之理.三阴交穴及子宫一级感觉神经元在脊神经节的节段分布(经穴相关的机理研究)[J].美国综合医学杂志,2000,2(6)：47.
[44]颜培宇.不同穴位针刺对免疫抑制大鼠神经肽相关基因表达及免疫调节作用的分子机制研究[D].黑龙江中医药大学博士学位论文,2010
[45]邹福清,赵海燕等.艾灸对急性髓系白血病化学治疗毒副反应的影响[J].河北中医,2011,33(8)：1201-1202
[46]江红柯.艾灸对运动训练大鼠糖原、血乳酸及运动能力的影响[J].河南大学学报(医学版),2011,30(4)：287-289
[47]恩和图娅.艾灸关元对化疗期生命体征影响的临床及实验研究[D].广州中医药大学博士学位论文,2010.
[48]王晓源,周忠光,郭迎喜.针刺“足三里”、“关元”穴对致衰小鼠睾丸组织抗氧化影响的实验研究[J].中医药信息,2010,27(2)：76-77
[49]河南焦作市第二人民医院针灸理疗科.针刺加TDP治疗慢性盆腔炎疗效观察[J].中国杜区医师·医学专业半月刊,2009,212(11)
[50]袁云霞.针刺治疗功能性痛经68例[J].上海针灸,2011,30(10)：698
[51]吴中朝,王玲玲.艾灸对老年人血浆睾酮、雌二醇的影响[J].中国针灸,1996,16(8)：27-28
[52]李晓泓,宋晓琳,张露芬,等.逆灸关元穴对自然更年期大鼠子宫HSP70,HSP70mRNA, SOD, NOS的影响[J].中华中医药杂志,2010,25(1)：46-49
[53]江瑜.艾灸关元、神阙穴对中期妊娠引产的疗效观察[J].中国中医基础医学杂志,2011,17(9)：1012-1013
[54]刘承,张海燕.艾灸治疗寒湿凝滞型原发性痛经疗效观察[J].世界针灸杂志,2011,21(3)：1-4
[55]张晓明,陈邦国.电针关元穴对脊髓不完全损伤大鼠尿动力学的影响[J]. 湖北中医学院学报,2009,11(3)：9-11
[56]高寅秋.电针促进腰麻后膀胱功能恢复疗效观察[D].中国中医科学院临床医学专业硕士学位论文,2011
[57]王丽芬,冯卫星.针刺配合葱白盐炒热敷治疗脑血管病后尿储留30例[J].2007,28(10)：1385
[58]尚艳杰,崔晓梅.关元、中极穴治疗慢性非细菌性前列腺炎的临床观察[J].黑龙江中医药,2008,2：39-40
[59]秦东霞.针刺治疗小儿神经性尿频42例[J].长春中医药大学学报2010,26(3)：411-412
[60]于竹力.针刺足运感区结合温针关元、中极治疗小儿遗尿[J].针灸临床杂志,2011,27(10)：16-17
[61]李改凤.针刺配合足底按摩治疗女性尿失禁40例[J].陕西中医,2007,28(3)：329
[62]郑燕生,赵铭峰,王伟.薄氏腹针对粘连性肠梗阻患者的止痛效果观察[C].针灸治疗痛证国际学术研讨会论文汇编2009,104-107
[63]邢金云.腹针防治含顺铂方案化疗所致恶心、呕吐的临床研究[D].北京中医药大学中医学专业硕士学位论文2011
[64]于磊.艾灸治疗慢性结肠炎20例[C].中国针灸协会针推结合专业委员会成立大会暨针灸教育与腧穴应用学术研讨会论文汇编,2010：341
[65]蔡焦生,杨树明,张爱萍.浅析艾灸关元穴治疗恶性腹水的机制[J].中国医药指南,2008,24(6)：314-315
[66]朱微微.针灸治疗厄逆50例分析[J].安徽卫生职业技术学院学报,2007,6(6)：33
[67]唐泗明,黄安清.艾灸关元穴治疗直肠脱垂57例分析[J].山西中医,2009,25(1)：25
[68]周金黄等.衰老、抗衰老、老年医学[M].北京：中国科学技术出版社,1993：54,160.
[69]伦新,荣莉.足三里、关元隔姜灸对老年人SOD,T细胞亚群的影响[J].新中医,2000,(4)：24.
[70]刘斌,王业秋等.针灸“足三里”及“关元”对大鼠皮肤光老化的防护作用[J].针灸临床杂志,2010,26(8)：56-57
[71]刘莹莹,等.艾灸对老年患者抗衰老的临床研究[J].河北医学,2009,15(4)：431-433
[72]马力佳,等.针灸对老年患者P16基因定量表达的影响[D].南京中医药 大学硕士学位论文,2010
[73]陈仲新.针刺配合饮食控制治疗单纯性肥胖疗效观察[J].中国针灸,2008,28(12)：888-890
[74]张梅.穴位埋线治疗更年期妇女肥胖的临床研究及其对血清生殖激素的影响[D].广州中医药大学学位论文,2008
[75]何立,等.针刺对单纯性肥胖症体重指数及腰臀围比的影响[J].中国针灸,2008,28(2)：95-97
[76]赵霞.针刺配合耳穴贴压治疗单纯性肥胖症的临床观察[D].黑龙江中医药大学硕士学位论文.2007
[77]贾燕亲,马文珠,赵静洁,李京真,陈彦安.艾灸减肥机制初探[C].2011中国针灸学会年会论文集,2011：729-733
[78]葛建军,王少锦,等.不同频率电针刺激对实验性肥胖大鼠减肥效应的影响[J].中国针灸,2007,27(8)：598-600
[79]黄文婷等.腹针对脑缺血再灌注大鼠运动功能和Nogo-A的影响[D].广州中医药大学硕士学位论文2011
[80]黄嫚,朱雄.腹针为主治疗肌张力低下型脑瘫验案二则[C].2011中国针灸协会年会论文集,2011
[81]孙景波,黄文婷.腹针引气归元法治疗中风病机制研究的思路与方法[C].
2011中国针灸协会年会论文集,2011：181-184
[82]陈真悟.腹针与体针治疗中风后抑郁症的疗效对比研究[D].广州中医药大学硕士学位论文2010
[83]陈晓军,方针.针刺“不寐四穴”为主治疗不寐21例[J].中国针灸,2009,29(4)：278
[84]汤耿民.腹部针刺法治疗慢性风湿性关节炎36例临床报告[J]世界中医骨科杂志,2010,11(1)：13-18
[85]张苗苗,孟凡迅等.大鼠任脉、督脉不同腧穴的针刺痛域相关性比较[J]四川中医,2007,25(6)：14-15
[1]王洪图主编《皇帝内经·素问·刺疟篇第三十六》[M]人民卫生出版社2006：248
[2]张慧.逆针对自然更年期大鼠生殖内分泌及免疫功能影响的实验研究[D]. 北京中医药大学硕士研究生学位论文,2005
[3]陈昌钧.兽医激光手术前麻醉[J].四川畜牧兽医,1980,2
[4]郑玲.“逆针灸”对更年期大鼠热休克蛋白及应激相关机制影响的研究[D].北京中医药大学硕士研究生学位论文,2007
[5]李晓泓,宋晓琳,徐莉莉,等.不同介入时机针刺三阴交穴对痛经大鼠下丘脑、垂体β-EP含量和HSP70表达的影响研究[J].中华中医药杂志,2010,25(9)：1456-1458
[6]刘丹,韩威,张迪.不同时间针刺对局灶性脑缺血再灌注大鼠BCL-2 Bax蛋白表达的影响[J].中华中医药学刊,2007,25(3)：622-623
[7]康世荚,熊星火,等.不同时间针刺对正常大鼠糖耐量的影响[J].上海针灸杂志,1996,15(4)：33-34
[8]叶飞,董军立,等.假性球麻痹后不同时间针刺风池穴的疗效比较[J].中国康复,2008,23(2)：88-89
[9]Selye H. Stress in health and disease. Boston:Butterworth,1976.18-45
[10]Laue L.etal.In:Chrousos GP, Lynn D&Gold PW. Eds. Mechanisms of Physical and Emotional Stress. New York and London:Pleuum press. 1988,225:235
[11]M izush ima Y, et al. Am J Physiol Regual In teger Comp Physiology,2000, 278(2):352-359.
[12]郭一闽.手术和术后镇痛对细胞因子的影响[J].临床医学,2008.28(4)：104-107
[13]何云云,何昆仑,刘春蕾.内质网应激与心血管疾病研究概述[J].生理科学进展,2011.42(6)：419-422
[14]B·M·舒贝克(苏)运动员身体负荷的免疫学研究[J].武汉体院译报,1987,4
[15]王茂叶.递增负荷运动对大鼠下丘脑一垂体一肾上腺皮质轴的影响研究[D].山东师范大学博士学位论文
[16]Laybutt DR, Preston AM, Akerfeldt MC, etal. Endoplasmic reticulum stress contritutes to beta cell apoptosis in type2 diabetes [J]. Diabetologia,2007, 50(4):752-763.
[17]Liu J, Jin X, Yu CH, etal. Endoplasmic reticulum stress involved in the course of lipogenesis in fatty acids-induced hepatic steatosis [J]. Gastroenterol Hepatol, 2010,25(3):613-618
[18]Fonseca SG, Burcin M, Gromada J, etal. Endoplasmic reticulum stress in beta cells and development of diabetes [J].Curr Opin Pharmacol,2009,9(6):763-770.
[19]Tabas I. The role of endoplasmic reticulum stress in the progression of atherosclerosis[J]. Circ Res,2010,107(7):839-850.
[20]劳宁.电针灸诱导脑缺血耐受作用的研究[D].第四军医大学学位论文2003
[21]周华,孙国杰等.针灸预处理对阿尔茨海默病大鼠学习记忆行为的影响[J].湖北中医药大学学报2011.13(1)：3-5
[22]Birnbaum Y, Hale SL Kloner RA. Ischemic preconditioning at a distance: reduction of myocardial infarctsize by partial reduction of blood suppy combined with rapid stimulation of the gastrocnemius muscle in the rabbit. Circulation 1997;96(5):1641-6
[23]李晓泓.针灸“治未病”与“针灸良性预应激假说”[J].北京中医药大学学报,2003,26(3)：82-85
[24]翟景慧,李晓泓,张露芬,等.“逆针”与“逆灸”对去卵巢大鼠血脂水平的影响[J].针刺研究,2005,30(2)：80-82
[25]张露芬,李晓泓,张慧,等.逆针关元穴对自然围绝经期大鼠血脂的调节作用[J].中国临床康复,2005,9(23)：132-133
[26]解秸萍,李晓泓,张露芬,等.“逆灸”调节大鼠围绝经期血脂、体重的效应规律[J].北京中医药大学学报,2006,29(1)：67-70
[27]李晓泓,解秸萍,张露芬,等.逆灸关元穴对自然围绝经期大鼠下丘脑雌激素受体α和促肾上腺激素释放激素及血浆促肾上腺激素的调节[J].中国临床康复,2006,10(7)：118-120
[28]段淑香,李亚东,李健男,等.针刺对围绝经期雌性大鼠血清雌二醇、睾酮、促性腺激素水平的影响[J].针灸临床杂志,2006,22(11)：44-45
[29]胡玲,汪惠丽,高忻洙,等.电针“关元”与“三阴交”对围绝经期模型大鼠性激素及下丘脑β-EP调整作用的比较[J].中国针灸,2004,24(9)：649-651
[30]张皓.针刺干预后围绝经期综合征患者临床症状及β一内啡肽的改变[J].国临床康复,2006,10(31)：1-3
[31]何玉伟,李晓泓.逆针灸对去卵巢大鼠生殖内分泌影响的研究[J].中华中医药学刊,2008,26(9)：1918-1920
[32]郑玲.“逆针灸”对更年期大鼠热休克蛋白及应激相关机制影响的研究[D].北京中医药大学硕士研究生学位论文
[33]李晓泓,郑玲.“逆针灸”干预对去卵巢大鼠下丘脑不同核团ER-α和ER-a mRNA表达水平的影响[J].中国针灸,2008,28(12)：918-921
[34]李晓泓,郑玲.逆针灸对去卵巢大鼠子宫保护效应的观察[J].针刺研究,2008,33(4)：229-234
[35]莫捷,李晓泓.逆针灸关元穴对12月龄自然更年期大鼠体质量增长率、下丘脑和卵巢瘦素含量的影响[J].天津中医药,2010,27(5)：383-385
[36]李晓泓,莫捷.“逆灸”关元穴对更年期大鼠体重增长率血脂和腹部脂肪胰岛素受体表达的影响[C].中国针灸学会临床分会第十七届全国针灸临床学术研讨会论文集,2009
[37]曹文忠,张莉,宋书邦,等.针灸预防中风后遗症复发的临床研究[J].中国针灸,2003,23(6)：317-320
[38]高俊虹,付卫星,晋志高,等.β-肾上腺素受体后信号转导通路介导电针预治疗抗缺血性心律失常的作用[J].中国针灸,2006,26(6)：431-435
[39]田代实,邓医字,王光海,等.针刺预处理对大鼠局灶性脑缺血的抗细胞凋亡作用[J].第四军医大学学报,2004,25(1)：27
[40]李晓泓,张露芬,郭顺根,等.逆灸对随后佐剂性关节炎大鼠早期及继发期炎性细胞因子和局部足肿胀的影响[J].中国临床康复,2005,9(15)：23-125
[41]杨运宽,刁灿阳,胡幼平,等.针灸防治肝癌概况[J].中国针灸,2002,22(9)：638-641
[42]徐正海.逆灸预防绝经后骨质疏松症的临床观察[J].中国中医药信息杂志,2001,8(1):79
[43]秦明,黄裕新,王景杰,等.预防性针刺足三里穴对冷应激大鼠胃粘膜损伤的影响[J].西南国防医药,2005,15(2)：33-135
[44]齐素云,王亚莉.电针预防广泛子宫切除术后尿潴留[J].河北医学,2005,11(4)：379-380
[45]施琴,针刺足三里、内关穴预防化疗胃肠道反应疗效观察[J].中医药临床杂志,2004,16(5)481
[46]程金莲,王麟鹏.“王氏夹脊穴”逆针灸治疗对脑卒中偏瘫痉挛状态117例随机对照观察[J].北京中医,2007.26(8)：467-469
[47]王樟连,陈利芳.逆针灸对缺血性卒中一级预防的价值探微[J].中华中医药学刊,2009.27(9)：1834-1836
[48]王少松.逆针灸针刺“王氏夹脊穴”防治中风后肌张力增高的临床研究[D].北京中医药大学硕士学位论文,2007
[49]高俊虹,晋志高.电针预处理改善心肌缺血性损伤的β1—肾上腺素能受体信号转导机制[D].中国中医科学院2003级博士研究生学位论文,2003
[50]黄秀容,袁青.逆针灸预防运动员关节损伤应用研究[D].广州中医药大学 硕士学位论文,2010
[51]李虹霖,孙远征.头穴透刺对运动性疲劳大鼠部分生化指标及氨基酸的影响[J].上海针灸杂志,2009.28(12)：738-740
[52]崔丽,孙国杰,周华,杜艳君.针灸预刺激对阿尔茨海默病模型大鼠学习记忆能力及海马区SOD和NOS的影响[J].湖北中医学院学报,2009.11(3)：6-8
[53]豁银成.针灸预刺激对AD大鼠神经元凋亡相关蛋Bc1-2,NF-KB的影响[D].湖北中医药大学硕士学位论文,2010
[54]莫捷,李晓泓等.逆针灸关元穴对12月龄自然更年期大鼠体质量增长率、下丘脑和卵巢瘦素含量的影响[J].天津中医药,2010,27(5)：383-385
[1]Vander EM, Horvath TL, Wiegant VM, et al. Evidence for a direct neuronal pathway from the suprachiasmatic nucleus to the gonadotropin-releasing hormone system:combined tracing and light and electron microscopic immunocytochemical studies. J Comp Neurol,1997; 384(8):569
[2]Mallick HN, Kumar VM. Gating of the dorsal penile nerve inputs by norepinephrine at the medial preoptic area in rats. Indian J Physiol Pharmacol, 2005;49(3):325
[3]SilerK, Theresa M, Grayson M,et al. GnRH and its anologues bind the human placental receptor[J]. Journalof the Society for Gynecologic Investigation,2001, 8(4):233-238.
[4]Wang L, Xie L P, HuangW Q,et al. Presence of gonadotropin releasing hormone (GnRH) and its mRNA in rat pancreas[J]. Molecular and Cellular Endocrinology,2001,172(1-2):185-191
[5]Huang W Q, Yao B, Sun L,et al. Immunohistochemical and in situ hybridization studies of GnRH and its receptor [J]. Life Science,2001,68(15):1727-1734.
[6]Chen H F, Jeung E B, Stephenson M,etal. Human peripheral blood mononuclear cells express gonadotropin releasing hormone (GnRH), GnRH receptor, and interleukin-2 receptor gamma-chain messenger ribonucleic acids thatare regulated by GnRH in vitro[J]. Journal of Clinical Endocrinology and Metabolism,1999,84:743-750
[7]StanislausD, Pinter JH, JanovickJA, et al. Mechanisms mediating multiple physiological responses to gonadotropin-releasing hormone[J].Molecular and Cellular Endocronology,1998,114:1-10
[8]张萍.促性腺激素释放激素系统与妇科肿瘤的研究进展[J].上海第二医科大学学报,2005,125(5)：535-536
[9]Smith MJ, Jennes L. Neural signals that regulate GnRH neurones directly during the oestrous cycle. J Reproduction,2001; 122(1):1
[10]Irahara M, Tamura T, Matuzaki T, et al. Orexin suppresses the pulsatile secretion of luteinizing hormone via beta endorphin. J Biochem Biophys Res, 2001;281(1):232
[11]Vincent P. Glial-neuronal-endothelial interactions are involved in the control of GnRH secrection [J]. JNeuroendocrin,2002,14
[12]金晓航,黄威权.促性腺激素释放激素免疫调节作用的研究进展[J].生理科学进展,2000,31(2)：169-172
[13]徐苓.老年妇女内分泌变化[J].中国实用妇科与产科杂志,2002,18(12)：721
[14]葛秦生,孙亦彬,刘士廉,等.临床生殖内分泌学[M].北京：科学技术文献出版社,2001,186-192,386-401
[15]何淑莹,申玉华,尤昭玲.多囊卵巢综合症LH及FSH的研究现状[J].中医药导报,2005,11(8)：82-84
[16]刘春杰,纪凤兰,董立珉等.二仙汤对更年期肾阳虚大鼠的影响[J].长春中医学院学报,2006,22(1)：61-62
[17]王洪珍,陈霁,李彩红.绝经后妇女血脂代谢的变化及激素替代治疗的影响[J].江苏大学学报(医学版),2006,16(2)：151-153
[18]Bellipanni G, Bianchi P, Pierpaoli Wet al. Effects of melatonin in perimenopausal and menopausal women:a randomized and placebo controlled study. Exp Gerontol,2001,36(2):297
[19]肖炜,邓虹珠,马云.益妇宁软胶囊治疗更年期综合征的实验研究[J].中国中药杂志,2003,28(3)：253-257
[20]Garrido-Gracia JC, Bellido C, Aguilar R, et al. Protein kinase C cross-talk with gonadotrope progesterone receptor is involved in GnRH-induced LH secretion. J Physiol Biochem,2006,62(1):35-42
[21]Stearns V, Hayes D F. Approach to menopausal symptoms in women with breast cancer [J]. Curr Treat Options Oncol 2002,3 (2):179
[22]Yun-Jun Ruan, Jian Qiu, Sai-Zhu Wu, et al. Effects of estrogen and its receptor on the production of nitric oxide in vascular endothelial cells, Chinese Journal of Clinical Rehabilitation,2004,8(6):1150-1151
[23]Shaw L, Taggart MJ, Austin C. Mechanisms of 17 beta-oestradiol induced vasodilatation in isolated pressurized rat small arteries[J].Cr J Pharmacol,2000, 129:555-565
[24]Razandi M, PedramA, Levin ER. Estrogen signalsto the preservation of endothelial cell form and function [J]. J Biol Chem,2000,275:38540-38546
[25]Sudoh N, Toba K, Akishita M, et al. Estrogen prevent soxidative stress induced endothelial cell apoptosis in rats[J]. Circulation,2001,103:724-729
[26]Stein DG. Brain damage, sex hormones and recovery:a new role for progesterone and estrogen[J]. Trends Neurosc 2001,7:386
[27]Burger HG, Dudley E, Mamers P, eta.l Early follicular phase serum FSH as a function of age:the role of inhibin B, inhibin A and estradiol.Climacteric,2000; 3(1):17
[28]Vaninetti S, Baccarelli A, Romoli R, et al. Effect of aging on serum gonadotropin levels in healthy subjects and patients with nonfunctioning pituitary adenomas. Europ J Endocr,i 2000; 142:144 [29]Wang Q, Santizo R, Baughman VL, et a.l Estrogen provides neuro protection in transient forebrain ischemia through perfusion-independent mechanisms in rats [J]. Stroke,1999,30(3):630
[30]李露斯,王兴华,范文辉.雌二醇对慢性脑缺血去卵巢大鼠脑病理改变的影响[J].第三军医大学学报,2002,24(12)：1391-1393
[31]汪艳丽,季其仁,肖玲玲等.围绝经期、绝经后妇女性激素水平的变化及其与糖、脂代谢的关系[J].安徽医学,2005,26(1)：27-28
[32]PaulS. Cooke, Afia. Role of Estrogens in Adipocyte Development and Function[J]. Exp Biol Med,2004,229:1127-1135.
[33]Wagner JD, Thomas MJ, Williams JK, et al. Insulin sensitivity and cardiovascular risk factors in ovariectomized monkeys with estradiol alone or combined with nomegestrol acetate. J Clin Endocrinol Metab,1998,83(3):896.
[34]王方杰,王吉听.胰岛素抵抗与糖尿病、肥胖、冠心病、脂代谢异常的相关性[J].实用心脑肺血管病杂志,2005,13(1)：53-55
[35]Sribastava M, Deal C·Osteoporosis in elderly:prevention and treatment[J].Clin Geriatr Med 2002,18(3):529-555
[36]Li NH,Ou PZ,ZhuHM, et al·Comparasion study on risk factors of osteopenia in the middle-aged and elderly men women population[J]·Zhongguo Linchuang Kangfu(Chin J Clin Rehabil)2002,6(3):382-383·
[37]苏汝堃,刘兴漠,邓颖辉.骨质疏松患者472例X线表现及骨密度分析[J].中国临床康复,2004,8(2)：4355
[38]Vogelvang TE, vander Mooren MJ, Mijatovic V. Hormone replacement therapy, selective estrogen receptor modulators, and tissue-specific compounds: cardiovascular effects and clinical implications. Treat Endocrinol,2004,3:105-115
[39]amada M, Irahara M,Maegawa M,et al.Transient increase in thelevels of T-helper 1 cytokines in postmenopausal women and the effects of hormone replacement.Therapy Gynecol and Obstet Invest,2001,52:82
[40]Kanda N,Tamaki K.Estrogen enhances immunoglobulin production by human PBM CS.J Allergy Clin Immunol,1999,103:282
[41]Raikkonen K, Mattbews KA, Kuller LH. The relationship between psychological risk attributes and the metabolic syndrome in healthy women: antecedent or consequence? Metabolism,2002,51(12):1573-1577
[42]Moe KE. Reproductive hormones, aging and sleep. Semin Reprod Endocrinol, 1999,17 (4):339
[43]NetzerN C, Eliasson A H, StrohlK P.Women with sleep apnea have lower levels of sex hormones·Sleep Breath,2003,7 (1):25
[44]PoloK P, Erkkola R, Irjala K et al.Effect of short-term transdermal estrogen replacement therapy on sleep:a randomized, double-blind crossover trial in postmenopausal women. Fertil Steri,1 1999,71 (5):873
[45]Crawford SL, Johannes CB, Stellato RK. Assessment of digit preference in self-reported year at menopause:chioce of an appropriate reference distribution. Am J Epidemiol 2002,156(7):676-683
[46]吴小立,张晋碚,温盛霖,等.围绝经期情绪障碍与雌二醇相关性的探讨[J].中国神经精神疾病杂志,2005,31(6)：455-457
[47]Drapier-Faure E, Azoulay C, Abramovici Y. Acceptability of continuous combined versus cyclical HRT:a French multi-centric randomized clinical study [J]. Gynecol Obstet Fertil,2005,33(7-8):498-504
[48]冷雯,陈沿东,陈岚华.雌孕激素阻断替代治疗围绝经期综合征研究初探[J].江苏医药,2004,30(5)：390
[49]朱建华,郭开华,徐杰.染料木素对去卵巢大鼠学习记忆能力的作用[J].中 国临床康复,2004,8(4)：650-651
[50]鲁兴梅.对雌激素来源和受体及其作用的再认识[J].卫生职业教育,2004,22：152-154
[51]UZAKI S, FUKAYA T, SUZUKI T, et al. Oestrogen receptor alpha and beta mRNA expression in human endometrium throughout the menstrual cycle[J]. Mol Hum an Repord,1999,5 (6):559-564.
[52]LAU KM,LASP INA M,LONG J, et al. Expression of Est2 rogen receptor (ER) 2alpha and ER2beta in nomal and malignant prostatic epithelial cell regulation by methylation and involvement in growth regulation [J]. Cancer Res,2000,60 (12):3175-3182
[53]王红,张元珍,刘文惠.雌激素替代治疗对去势大鼠阴道组织雌激素受体表达的影响[J].医药导报,2008,27(11)：1304-1306
[54]张吉强,蔡文琴.脑内雌激素受体的研究进展[J].局解手术学杂志,2009,18(1).
[55]Simoncini T, Mannella p, Fornari L, et al. Genomic and nongenomic effects of estrogens on endothelial cells[J]. Steroids,2004,69(8-9):537-542
[56]张吉强,蔡文琴.雌激素受体研究进展[J].生理科学进展,2001,32(1)68-70.
[57]Nagata H, Nozaki M, Nakano H. Short-term combinational therapy of low-dose estrogen with selective serotonin re-uptake inhibitor(fluvoxamine) for oophorectomized women with hot flashes and depressive tendencies [J]. J Obstet Gynaecol Res,2005,31(2)
[58]李红,任林,李灿东,林雪娟.围绝经期综合征肝气郁结证患者雌激素受体β基因多态性分析[J].中华中医药杂志,2010,25(6)：830-832
[59]胡佳佳,周伯荣,秦媛怡,张慧敏。女性围绝经期抑郁症与雌激素受体β基因多态性的相关性研究[J].安徽医科大学学报,2011,46(6)：562-565
[60]Miehael R. Soules, Sherry Sherman, Estella P ott, et al. Stages of Reproduetive Aging OrkshoP(STRAW)[J].M men,5Health&Gender-based Medicine. 2001;10(9):843-849.
[61]Janssenl, Heymsfield SB, Allison DB, et al. Body mass index and waist circumference independently contribute to the prediction of non abdominal, abdominal subcutaneous, and visceral fat [J].Am J Clin Nutr,2002,75(4):683
[62]Henry G. Burger, EmmaC. Dudley, JiShengCui, et al. A prospective longitudinal study of serum testosterone, dehydroe Piandrosterone sulfate, and sex hormone binding globulin levels through the menopause transition[J].Clin Endocrinol Metab2000,85:2832-2838
[63]Takahiro mizutani,yoshinobu nishikawa,hiroshi adachi,et al.Identification of estrogen receptor in human adipose tissue and adipocytes.J Chin Endocrionl Metab,1991,78:950-954.
[64]Wade GN, Gray JM.Cytoplasmic 17-β[3H]estradiol binding in rat adipose tissues.Endocrinology,1978,103:1695-1701.
[65]Gray JM, Wade GN.Cytoplasmic progestin binding in rat adipose tissues.Endocrinology,1979,104:1377-1382.
[66]SB. Pedesen, PS. Hansen, S Lund, et al. Identification of estrogen receptors and estrogen recepter mRNA in human adipose tissue. Eur J Clin Invest,1996, 26:262-269.
[67]赵文燕,李英敏.脂肪细胞雌激素受体的测定及表达水平的研究[J].河北医药,2004,26(9)：716-717
[68]Homma H, Kurachi H, Nishio Y, et al. Estrogen sup2 p resses t ranscrip tion of lip op rotein lip ase gene [J]. J Bi2 ological Chemist ry,2000,275:11404-11411.
[69]Heine PA, Taylor JA, Lwamot o GA, et al. Increased adipose tissue in male and female estrogen receptor2aknockout mice [J]. Proc Natl Acad Sci USA,2000, 97 12729-12734.
[70]赵文燕,董耀,仇树林.脂肪细胞雌激素受体的表达及其与人体脂肪分布的相关性[J].中国美容医学,2005,14(5)：534-536
[71]朱晓晖,王军,印彤.雌激素干预影响腹腔脂肪积聚的实验研究[J].中国临床药理学与治疗学,2003,8(6)：669-672
[72]Edwin LB, John AG. Disorders of lipid metabolism[A]. In:Robert HW, ed. Textbook of Endocrinology[M]. Philadelphia:PA. W.B.Saunders,1992:1367
[73]Mattiasson I, Rendell M, Tomquist C, et al. Effects of estro-gen replacement therapy on abdominal fat compartments as related to glucose and lipid metabolism in early postmenopausal women[J]. HormMetab Res,2002;34:583-8
[74]Perrone G, LiuY, Capri O, et al. Evaluation of the body composition and fat distribution in long-term users of hormone replacement therapy. Gynecol Obstet Invest.1999,48(1):52-55.
[75]李琦.绝经后早期女性体脂分布的特点及其与雄激素水平和骨密度的相关 性[D].复旦大学硕士学位论文,2010