大豆对食叶性害虫抗性的鉴定和抗虫相关基因的克隆及其CAPS标记
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摘要
大豆食叶性害虫是影响大豆产量和品质的因素之一。本文的研究目的之一是筛选鉴定大豆抗感虫材料。采用室内喂养斜纹夜蛾的方法，利用网室采集的58份大豆资源的叶片，室内喂养刚孵化的斜纹夜蛾幼虫。根据虫体的反应，以幼虫重和蛹重为指标，鉴定了大豆资源对斜纹夜蛾单一虫种的抗性表现。鉴定结果表明：两指标在各材料间差异极显著；且幼虫重和蛹重间相关显著。综合2001年和2002年的抗性鉴定结果根据标准品种分级法所划分的抗性等级筛选出4份表现高抗的材料：黄皮小青豆、日本、矮杆黄、P1227687；表现高感的材料3份：监利牛毛黄、沔阳白毛豆、大浦大粒黄。可作为抗性鉴定的标准材料，用于抗性分级；也可作为抗感亲本，用于大豆对斜纹夜蛾抗生性的遗传研究或育种应用；也可作为克隆抗虫基因和鉴定基因功能的材料。
本研究的另一个目的是克隆抗虫基因。根据大豆KUNITZ型胰蛋白酶抑制剂基因保守域设计特异性引物，用同源序列克隆法从大豆材料Lamar(抗)和89-29(感)中分离KUNITZ型胰蛋白酶抑制剂基因。序列分析表明，该基因与已报导的序列高度同源：其核苷酸序列及推导的编码氨基酸序列的同源率分别为99.3％和99.1％。对从抗感材料获得的蛋白酶抑制剂基因进行分析，可知抗感材料间存在着3个核苷酸的差异并引起2个氨基酸残基的变化。
根据大豆凝集素基因设计特异性引物，从大豆材料皖82-178(抗)和通山薄皮黄豆甲(感)中获得凝集素基因。序列分析表明，皖82-178中克隆到的基因片段大小为999bp，没有内含子，编码一条长为285个氨基酸、分子量约31KD的肽链。其中N-端31个氨基酸是信号肽。与已报道的序列高度同源：其核苷酸序列及推论的编码氨基酸序列的同源率分别为99.6％和99.3％。系统进化分析表明，大豆凝集素基因最先与亲缘关系较近的其他豆科植物先聚类，再和亲缘关系较远的禾本科、十字花科等植物聚类。说明了这些植物间的亲缘关系，也说明对基因表达产物功能的推测是可靠的。通山薄皮黄豆甲中获得的lec序列编码一条长为253个氨基酸的肽链。结构分析表明只含有Legume lectin beta domain，而皖82-178中得到的lec基因含有Legume lectin beta domain和Legume lectin alpha domain。这些变化对多肽的抑制活性可能会
有影响，但结果还有待于进一步验证。
根据大豆胰蛋白酶抑制剂基因设计特异性引物，从科丰l号(杭)、1 1 38一2(感)
组合的重组自交系的亲本中分别获得大豆蛋白酶抑制剂基因.经序列分析表明，克隆
到的基因片段大小为1 376bP，含835bp的内含子。经分析亲本间有酶切位点(B stBI)
的差异.从185个家系中随机筛选了104个家系进行以Ps分析.但根据所得的分子
数据和现有养虫的杭性数据进行分析，发现还不能将此C人PS标记与杭虫性结果相联
系，需要进一步研究.
Soybean leaf-feeding insects affect the yield and quality of soybean. One objective of this study was to screen resistant and susceptible soybean accessions. The 58 accessions were tested for the antibiosis to cotton worm (Prodenia litura Fabricius) under inoculation condition according to the 10 days larval and pupal weight. The result indicated that the larval weight and pupal weight were all significantly different among varieties. Low larval and pupal weight indicates the high level of antibiosis. Larval weight was positively related to pupal weight. According to the result of 2001 and 2002, four genotypes, N20922, N21565, N21249 and N21551 were identified to be highly resistant to cotton worm; Three genotypes, N3108, N4029-3 and N3155-1 were identified to be highly susceptible to cotton worm. These genotypes can be used in inheritance study, breeding program and functional study.
In this study, Soybean kunitz trypsin inhibitor was cloned through candidate gene homology-based method by specific primers designed on the basis of KTi3. DNA sequence analysis indicates that the cloned DNA fragment composed of 700bp has 99.3%homologies in nucleotide sequence and 99.1% in amino acid sequence respectively compared with previously published sequence. There are three bases differences, which lead to two amino acids changes between sequence of KTI from Larma and 89-29.
With the same method, the soybean lectin was also cloned. The sequence from Wan82-178 show that the cloned fragment is 999bp. Like most plant lectin genes, there is no intron in the amplified sequence. The open reading frame of the fragment codes a 31KD polypeptide, which contains 285 amino acid residues. There is a signal sequence with 31 amino acid residues at its NH2 terminus. The cloned DNA fragment has 99.6% and 99.3%homologies in nucleotide sequence and amino acid sequence compared with Led. Molecular evolution studies of Lec reveals that Lec clusteres with legumes first, then
clusteres with Gram ineae and mustard family. This shows the genetic relationship among plants and suggests that the inference is reliable about the function of the product of gene. The sequence from Tong Shan bo pi huang dou jia show that the cloned fragment is 1000bp. The open reading frame contains 253 amino acid residues. Structure analysis show that it only contains Legume lectin beta domain. And the sequence from Wan 82-17S contains Legume lectin beta domain and Legume lectin beta domain. These changes maybe affect the activity of polypeptide. But this requires further study to be carried out.
The soybean trypsin inhibitor was obtained from two parents of recombinant inbred population (RIL) from the cross of kefeng No 1 (resistance) and 1138-2(susceptible). DNA sequence analysis indicates that the cloned fragment is 1376bp with an 835bp intron. After the BstB I digestion, the digestion products showed polymorphism between the two parents. 104 lines were screened randomly from RIL to digest the PCR products with BstB I. The data analysis showed that the current CAPS marker data are not relevant to the resistance data. This requires further study to be carried out
本研究的另一个目的是克隆抗虫基因。根据大豆KUNITZ型胰蛋白酶抑制剂基因保守域设计特异性引物，用同源序列克隆法从大豆材料Lamar(抗)和89-29(感)中分离KUNITZ型胰蛋白酶抑制剂基因。序列分析表明，该基因与已报导的序列高度同源：其核苷酸序列及推导的编码氨基酸序列的同源率分别为99.3％和99.1％。对从抗感材料获得的蛋白酶抑制剂基因进行分析，可知抗感材料间存在着3个核苷酸的差异并引起2个氨基酸残基的变化。
根据大豆凝集素基因设计特异性引物，从大豆材料皖82-178(抗)和通山薄皮黄豆甲(感)中获得凝集素基因。序列分析表明，皖82-178中克隆到的基因片段大小为999bp，没有内含子，编码一条长为285个氨基酸、分子量约31KD的肽链。其中N-端31个氨基酸是信号肽。与已报道的序列高度同源：其核苷酸序列及推论的编码氨基酸序列的同源率分别为99.6％和99.3％。系统进化分析表明，大豆凝集素基因最先与亲缘关系较近的其他豆科植物先聚类，再和亲缘关系较远的禾本科、十字花科等植物聚类。说明了这些植物间的亲缘关系，也说明对基因表达产物功能的推测是可靠的。通山薄皮黄豆甲中获得的lec序列编码一条长为253个氨基酸的肽链。结构分析表明只含有Legume lectin beta domain，而皖82-178中得到的lec基因含有Legume lectin beta domain和Legume lectin alpha domain。这些变化对多肽的抑制活性可能会
有影响，但结果还有待于进一步验证。
根据大豆胰蛋白酶抑制剂基因设计特异性引物，从科丰l号(杭)、1 1 38一2(感)
组合的重组自交系的亲本中分别获得大豆蛋白酶抑制剂基因.经序列分析表明，克隆
到的基因片段大小为1 376bP，含835bp的内含子。经分析亲本间有酶切位点(B stBI)
的差异.从185个家系中随机筛选了104个家系进行以Ps分析.但根据所得的分子
数据和现有养虫的杭性数据进行分析，发现还不能将此C人PS标记与杭虫性结果相联
系，需要进一步研究.
Soybean leaf-feeding insects affect the yield and quality of soybean. One objective of this study was to screen resistant and susceptible soybean accessions. The 58 accessions were tested for the antibiosis to cotton worm (Prodenia litura Fabricius) under inoculation condition according to the 10 days larval and pupal weight. The result indicated that the larval weight and pupal weight were all significantly different among varieties. Low larval and pupal weight indicates the high level of antibiosis. Larval weight was positively related to pupal weight. According to the result of 2001 and 2002, four genotypes, N20922, N21565, N21249 and N21551 were identified to be highly resistant to cotton worm; Three genotypes, N3108, N4029-3 and N3155-1 were identified to be highly susceptible to cotton worm. These genotypes can be used in inheritance study, breeding program and functional study.
In this study, Soybean kunitz trypsin inhibitor was cloned through candidate gene homology-based method by specific primers designed on the basis of KTi3. DNA sequence analysis indicates that the cloned DNA fragment composed of 700bp has 99.3%homologies in nucleotide sequence and 99.1% in amino acid sequence respectively compared with previously published sequence. There are three bases differences, which lead to two amino acids changes between sequence of KTI from Larma and 89-29.
With the same method, the soybean lectin was also cloned. The sequence from Wan82-178 show that the cloned fragment is 999bp. Like most plant lectin genes, there is no intron in the amplified sequence. The open reading frame of the fragment codes a 31KD polypeptide, which contains 285 amino acid residues. There is a signal sequence with 31 amino acid residues at its NH2 terminus. The cloned DNA fragment has 99.6% and 99.3%homologies in nucleotide sequence and amino acid sequence compared with Led. Molecular evolution studies of Lec reveals that Lec clusteres with legumes first, then
clusteres with Gram ineae and mustard family. This shows the genetic relationship among plants and suggests that the inference is reliable about the function of the product of gene. The sequence from Tong Shan bo pi huang dou jia show that the cloned fragment is 1000bp. The open reading frame contains 253 amino acid residues. Structure analysis show that it only contains Legume lectin beta domain. And the sequence from Wan 82-17S contains Legume lectin beta domain and Legume lectin beta domain. These changes maybe affect the activity of polypeptide. But this requires further study to be carried out.
The soybean trypsin inhibitor was obtained from two parents of recombinant inbred population (RIL) from the cross of kefeng No 1 (resistance) and 1138-2(susceptible). DNA sequence analysis indicates that the cloned fragment is 1376bp with an 835bp intron. After the BstB I digestion, the digestion products showed polymorphism between the two parents. 104 lines were screened randomly from RIL to digest the PCR products with BstB I. The data analysis showed that the current CAPS marker data are not relevant to the resistance data. This requires further study to be carried out
引文
1．曹骥编．1984．作物抗虫原理及应用．北京：科学出版社
2．程林梅、孙毅、岳焕荣．2001．大豆生物工程研究进展．大豆科学20(1)：66-70
3．崔章林、盖钧镒．吉东风，任珍静，赵团结．1996．大豆种质资源对南京地区重要食叶性害虫抗性的鉴定．中国农业科学，29(4)：95-96
4．崔章林，盖钧镒，吉东风，任珍静．1997．大豆种质资源对食叶性害虫抗性的鉴定．大豆科学，16(2)：93-102
5．崔章林，盖钧镒，吉东风，任珍静．1997．南京地区大豆食叶性害虫种类调查与分析．大豆科学，16(1)：12-20
6．崔章林，盖钧镒．1996．大豆抗豆秆黑潜蝇研究进展．中国油料，18(3)：79-81
7．崔章林．盖钧镒．1996．大豆抗食叶性害虫研究进展．大豆科学，15(2)：149-158
8．崔章林，盖钧镒．1997．大豆种质资源对食叶性害虫抗性的鉴定．大豆科学，16(2)：93-102
9．崔章林．1995．南京地区大豆叶食性害虫重要种类分析与抗源鉴定．硕士学位论文，南京农业大学
10．邓九生，张大鹏，陈大明．2000．荔枝铜锌超氧物歧化酶基因的克隆与序列分析．广西农业生物科学．19(2)：73-76
11．董朝蓬，杜林方，段真2003．植物凝集素研究进展．天然产物研究与开发．15(1)：71-76
12．盖钧镒，崔章林．1997．大豆抗食叶性害虫育种鉴定方法与指标．作物学报，23(4)：400-407
13．盖钧镒，夏基康，崔章林，任珍静，浦奉华，吉东风．1989．我国南方大豆资源对豆秆黑潜蝇抗性的研究．大豆科学，8(2)：115-121
14．盖钧镒主编．2000．试验统计方法．北京：中国农业出版社
15．高文娜．2003．小麦抗锈病基冈分子标记．硕士学位论文．西农农业大学
16．高燕会，李润植，毛雪，李彩霞．2000．植物凝集素的防卫功能及其研究进展世界农业250(2)：33-35
17．高越峰，朱祯，肖桂芳，朱玉，吴茜，李向辉．1998．大豆Kunitz型胰蛋白酶抑制剂基因的分离及其在抗虫植物基因工程中的应用．植物学报40(5)：405-411
18．高越峰，朱祯，朱玉，肖桂芳，李向辉．1997．大豆Kunitz型胰蛋白酶抑制剂基因的克隆及其转基因烟草抗虫性初探．高技术通讯，(9)：5-9
19．郭凤英，沙广乐．1997．蛋白酶抑制剂研究进展．河南职技师院学报．25(4)：5-7
20．顾克余．1999．水稻汕优63及其亲本差异基因表达与克隆研究．博士学位论文，南京农业大学
21．郝峥嵘，刘庆法，季昀，叶鸣明，陈永青，沈大棱．1999．稻胚凝集素基因的克隆、序列分析及表达．西北植物学报．19(2)：183-189
22．J．萨姆布鲁克，D．W．拉塞尔．2002．分子克隆实验指南(第三版)．科学出版社
23．贾璇．2003．小麦抗叶锈基因Lr38的AFLP分子标记．硕士学位论文．河北农业大学
24．江树业，陈启锋，方宜钧，李维明，吴为人．2000．植物基因分离方法及其评述．福建农业大学学报．29(3)：261-268
25．李鸣．2003．AFLP技术在甘蔗品种鉴定和亲缘关系研究上的应用．硕士学位论文．广西大学
26．李玉英．2003．荞麦蛋白酶抑制剂基因的克隆及序列分析．硕士学位论文．山西大学
27．梁峰，常团结．2002．植物凝集素的研究进展．武汉大学学报．48(2)：232-238
28．柳武革，薛庆中．2000蛋白酶抑制剂及其在抗虫基因工程中的应用．生物技术通报．1：20-25
29．路子显，常团结．朱祯．2002．植物外源凝集素及其在植物基因工程中的应用．生物：工程进展．22(2)：3-9
30．南相玉，刘文萍，刘丽艳，吕晓波．何云霞，卫志明．1998．PEG介导BT基因转化大豆原生质体获转基因植株．大豆科学，17(4)：326-329
31．潘科，黄炳球，侯学文．2002．植物凝集素在病虫害防治中的研究进展．植物保护．28(4)：42-44
32．彭玉华．1996．大豆抗食叶性害虫研究概况．大豆通报，(1)：26
33．钱海丰，陈小荣，田振涛，薛庆中．2002蛋白酶抑制剂在抗虫基因工程中的应用．生命科学．14(5)：308-310
34．钦俊德著．1987．昆虫与植物的关系．北京：科学出版社
35．曲晓华，浦冠勤．2003．蛋白酶抑制剂的研究与应用．蚕桑茶叶通讯．1：19-22
36．苏军．蛋白酶抑制剂基因的杀虫机理及其应用．福建果树．100：19-21
37．孙祖东．盖钧镒．1999．大豆对食叶性害虫抗性的研究．中国农业科学，32(增刊)：81-88
38．孙祖东，盖钧镒．1999．大豆对食叶性害虫抗性机制的研究．中国油料作物学报，21(2)：60-64
39．孙祖东．盖钧镒．1999．大豆抗食叶性害虫遗传的初步研究．大豆科学．18(4)：300-304
40．孙祖东，盖钧镒．1999．大豆品种对斜纹夜蛾抗性机制初探．植物保护学报．26(4)：329-332
41．孙祖东，盖钧镒．2000．大豆抗斜纹夜蛾幼虫的遗传研究．作物学报，26(3)：341346
42．孙祖东，杨守臻，陈怀珠，韦德卫．2001．南宁大豆食叶性害虫调查．广西农业科学，(2)：104-106
43．孙祖东．1997．南京地区大豆对食叶性害虫抗性的鉴定、机制和遗传的研究．博士学位论文，南京农业大学
44．谈建中，楼程富，平野久．2000．大豆特殊种子蛋白豆类胰岛素基因的克隆与序列分析．浙江大学学报．26(1)107-110
45．王斌，翁曼丽．1996．AFLP的原理及其应用[J]．杂交水稻．(5)：27-30．
46．王春明，安井秀，古村醇，苏昌潮，翟虎渠，万建民．2003．水稻叶蝉抗性基因回交转育和CAPS标记辅助选择．中国农业科学．36(3)：237-241
47．王得元，李颖，黎振兴，黄爱兴．1998．DNA分子标记在作物杂种优势研究中的应用．西北农
业学报．7(3)：94-97
48．王慧．2003．大豆对食叶性害虫抗性的遗传及相关基因的SSR标记和定位的研究．硕士学位论文，南京农业大学
49．王玲．2002．辣椒遗传多样性的RAPD分析．硕士学位论文．福建农林大学
50．王伟，朱祯等．1999．双价抗虫基因陆地棉转化植株的获得．植物学报．41(4)：384-388
51．于新力，彭学贤．2001．香蕉果实成熟相关基因ACO1启动子区的克隆及其功能初探．生物工程学报．17(4)：428-431
52．王志斌，李学勇，郭三堆．1998．植物凝集素与抗虫基因工程．生物技术通报2：5-10
53．王白章．李杨瑞，张树珍等．2003．甘蔗ACC氧化酶基因片段的克隆与序列分析．遗传学报，30(1)：62-69
54．吴谡琦等．2001．分子标记技术的进展及其应用．高科技通讯，1：99-103．
55．吴业春．2003．大豆对食叶性害虫抗性鉴定及对斜纹夜蛾抗生性的遗传研究．硕士学位论文，南京农业大学
56．谢可方，董爱武，忻骅等．2002大豆KIJNIT7型胰蛋白酶抑制剂的稳定性及抗虫性研究．复旦学报．41(6)：631-634
57．徐春晓．2000．大豆对食叶性害虫抗性的植株反应和虫体反应及其在资源鉴定、遗传与选育中的应用．硕士学位论文，南京农业大学
58．徐香玲等．1997．Ti质粒介导的B．t．K—δ内毒素蛋白基因转化大豆的初步研究[J]．大豆科学，16(1)：9-11．
59．颜辉煌．1990．大豆对豆秆黑潜蝇抗选性、抗生性及耐虫性的一些研究．硕士学位论文，南京农业大学
60．杨辉霞，王芳，单雷，毕玉平．2003．花生球蛋白基因片段的克隆．花生学报．32(3)：15-18
61．杨素欣，冯献忠，刘平林，何玉科．2000．甘蓝中BcpLH同源基因的克隆及其序列分析．植物生理学报．26(4)363-368
62．伊佟明，黄敏仁．1997．AFLP分子标记及其在植物育种上的应用[J]．生物工程进展．17(1)：6-11．
63．余波澜，黄朝峰，周文娟，张文俊．2001．大麦1H特异性CAPs标记和ASA标记的创制．遗传学报28(6)：550-555
64．翟凤林等译(Maxwell F．G．等著)．1985．植物抗虫育种．北京：农业出版社
65．曾庆平，何振宇．1994．植物基因多态性的系统分类学研究(Ⅱ)凝集素基因的分子进化．长沙水电师院自然科学学报．9(3)：299-305
66．詹秋文，盖钧镒．1999．大豆资源对食叶性害虫抗性的差异反应及种质鉴定．安徽农业技术师范学院学报．13(4)：1-6
67．詹秋文．1999．大豆对食叶性害虫抗性植株反应的资源鉴定、遗传与选育．硕士学位论文，南京农业大学
68．詹秋文．2000．大豆种质资源对斜纹夜蛾(PRODENIA LITURA)抗性的鉴定．应用与环境生物学报．6(1)：18-23
69．张宝红．丰嵘编著．2000．棉花的抗虫性与抗虫棉．北京：中国农业科技出版社
70．张良佑，吴洪基等．1998．转基因水稻的抗虫性初探．华南农业大学学报．19(2)：4-7
71．张玉静．2000．分子遗传学．北京：科学出版社
72．张宗炳，曹骥主编．1990．害虫防治：策略与方法．北京：科学出版社
73．赵东，刘祖生．奚彪．2001．茶树多酚氧化酶基因的克隆及其序列比较．茶叶科学．21(2)：94-98
74．赵洪锟，李启云、王玉民，庄炳昌．2002．大豆Kunitz型胰蛋白酶抑制剂(SKTI)研究进展．大豆科学．21(3)：218-222
75．周明群编．1992．作物抗虫性原理及应用．北京：北京农业大学出版社
76．周国岭，杨光圣，傅延栋．2001．基因克隆技术．华中农业大学学报．20(6)：584-592
77．朱成松．顾和平，陈新．1998．大豆对食叶性害虫抗性的自然鉴定．江苏农业科学．2：33-35
78．朱成松．顾和平，陈新．2002．大豆抗虫基因工程研究进展．大豆通报．6：17-18
79．朱成松，张宝龙，王学军．1999．大豆对食叶性害虫的抗性与农艺性状的关系．中国油料作物学报，21(1)：59-62
80．祝水金，汪若海．1995．植物蛋白酶抑制因子及其在植物抗虫基因工程中的应用．生物技术．5(3)1-5
81．朱玉，吴茜，高越峰，徐鸿林，刘春明，周兆斓，朱祯，李向辉．1997．雪花莲外源凝集素基因的克隆、序列分析和植物表达载体的构建．农业生物技术学报。5(4)：331-338
82. Awadallah, W.H., M. F. Lufallah, E.M.A. El-Moneim, M.F. Ei-Metwally, M.Z. Hassan. 1990. Evaluation of some soybean for their resistance to the cotton leaf worm, Spodoptera littoralis boid. Agricultural Research Review, 68(1).
83. Beach, R.M., J.W. Todd, H.R. Boerma. 1988. Relative resistance of three soybean plant introductions to the soybean looper and velvebean caterpillar in Georgia. Journal of Entomological Science, 23(4):399-401.
84. Bostwick D E, Skaggs M I, Thompson G A. 1994.Organization and Characterization of Cururbita Phloem Lectin Genes. Plant Mol Boil. 26:887-897
85. Brier, H.B., D.J. Rogers. 1991. Leaf-feeding resistance to six Australian noctuids in soybean. Crop Protection, 10(4):320-324.
86. Burton, J.W., et al. 1986. Registration of insect resistant soybean germplasm lines N80-53201, N79-2282, N80-50232. Crop Science, 26(1)212.
87. Copper, R.L., et al. 1988. Registration of Mexican bean beetle resistant germplasm line HC83-1293. Crop Sci., 28:1037-1038.
88. Cooper R.L., R.B. Hammond. 1998. Registration of insect-resistant soybean germplasm lines HC95-24MB and HC95-15MB. Crop Sci. 39(2): 599
89. Prey K.J. 1981. Plant Breeding. Iowa State University Press
90. Gary, D. J., et al. 1985. Evaluation of soybean plant introductions for resistance to foliar feeding insects. Journal of the Mississippi Academy of Sciences, 30:67-82
91. Gai J.Y., J.K. Wang. 1998. Identification and estimation of a QTL model and its effects. Theor. Appl. Genet. 97:1162-1168
92. Gai J.Y., Z.D. Sun, Z.L. Cui. 1996. Studies on resistance mechanism of soybeans to leaf feeders. Soybean Genetics Newsletter. 1996, 25:55-56
93. Gary D.J., L. Lambert, J.D. Ouzts. 1985. Evaluation of soybean plant introductions for resistance to foliar feeding insects. Journal of the Mississippi Academy of Sciences. 30:67-82
94. Haq M., Karim ANMR, S. Alam. 1984. Preliminary study of varietal reaction of soybean cultivars to leaf defoliators. Tropical Grain Legume Bulletin. 29:35-37
95. Hartwig E.E., S.G. Turnipseed T.C. Kilen. 1984. Registration of soybean germplasm line D75-10169. Crop Sci. 24:214-215
96. Hartwig, E.E., and T.C.K. 1990. Registration of soybean cultivar Lamar. Crop Sci., 30(1):231.
97. Hilder V.A, Gatehouse A.M.R, Sheerman S.E. 1987. Nature. 330:160-165
98. Hilder V.A. Powell K.S. Gatehouse A.W.R. et al. 1995. Expression of Snowdrop Lectin in Transgenic Tobacco Plants Results in Added Protection Against Aphids. Transgenic Research. 4:18-25
99. Hopins J.D. et al. 1983. Distribution of bean pod mottle virus in Arkansas soybean as related to the bean leaf beetle, Cerotoma trifurcata, (Coleoptera: Chrysomelidae) Population. Environ. Entomol. 12(5): 1564-1567
100. Jivaid I., J.M. Joshi, R.B. Dadson, M. Nobaakht. 1991. Leaf feeding resistance in soybean breeding lines to corn earworm (Heliothis zes Boddie). Soybean Genetics Newsletter. 18:271-274
101. Jofuku K.D., RB. Goldberg. 1989. Kunitz trypsin inhibitor genes are differentially expressed during the soybean life cycle and in transformed tobacco plants. Plant Cell. 1(11): 1079-1093
102. Johnson R. et al. 1989. Proc natl Acad Sci USA. 86:9871
103. Joudrier P.E., D.E. Foard, L.A. Floener, B.A. Larkins. 1987. Isolationand sequence of cDNA encoding the soybean protease inhibitors PI Ⅵ and C-Ⅱ. Plant Mol. Biol. 10:35-42
104. Kilen T.C., J.H. Hatchett, E.E. Hartwig. 1977. Evaluation of early generation soybeans for resistance to soybean looper. Crop Sci. 17:397-398
105. Kilen T.C., L. Lambert. 1986. Evidence for different genes controlling insect resistance in three soybean genotypes. Crop Sci. 26(6): 869-871
106. Kilen T.C., L. Lambert. 1993. Registration of three glabrous and three pubescent soybean germplasm lines susceptible (D88-5320, D88-5295), moderately resistant (D88-5328, D88-5272) or resistant(D90-9216, D90-9220) to foliar-feeding insects. Crop Sci. 33(1): 215
107. Kilen T.C., L. Lambert. 1998. Genetic control of insect resistance in soybean germplasm PI 417061. Crop Sci. 38:652-654
108. Konami Y,Yamamoto K, Osawa T. 1991. The Primary Structures of two Types of the Ulex Europeus Seed Lectin. Jounal of Biochemistry. 109:650-658
109. Kraemer M.E., M. Rangappa, W. Gade, P.S. Benepal. 1987. Induction of tripsin inhibitors in soybean leaves by Mexican bean beetle (Coleoptera: Coccinellidae) defoliation. J. Econ. Entomol. 80(1): 237-241
110. K S Powell, A M R Gatehouse, et al. 1995. Antifeedant effects of plant lectins and enzyse on the adult stage of the rice brown plant hopper, Nilaparvata lugens. Entomologia Experimentalis et Applicatan. 75:51
111. Lambert L., L.G. Heatherly. 1991. Soil water potential effects on soybean looper feeding on soybean leaves. Crop Sci. 31(6): 1625-1628
112. Lambert L., R.M. Beach, T.C. Kilen. 1992. Soybean pubescence and it's influence on larval development and oviposition preference of Lepidopterous insects. Crop Sci. 32:463-466
113. Lambert L., T.C. Kilen. 1984. Influence of three soybean plant genotypes and their F, intercrosses on the development of five insect species. J. of Econ. Entomol. 77(3): 622-625
114. Lambert L., T.C. Kilen. 1984. Insect resistance factor in soybean PI's 229358 and 227687 demonstrated by drafting. Crop Sci. 24:163-165
115. Lambert L., T.C. Kilen. 1984. Multiple Insect resistance in several soybean genotypes.
Crop Sci. 24:887-890
116. Lin H, M. Kogan, D. Fischer. 1990. Induced resistance in soybean to the Mexican been beetle (Coleoptera: Coccinellidae): comparisons of inducing factors. Environmental Entomology. 19(6): 1852-1857
117. Luedders V.D., W.A. Dickerson. 1977. Resistance of selected genotypes and segregating populations to cabbage looper feeding. Crop Sci. 17:395-396
118. Mbrahtu T. 1986. Efficiency of different selection and screening methods to identify productive soybean breeding lines resistant to Mexican bean beetle. Dissertation Abstracts International. 47(5): 1800
119. Mrahtu T., W.J. Kenworthy, T.C. Elden. 1990. Genetic study of resistance to Mexican bean beetle in soybean lines. Journal of Genetics and Breeding. 44(1) :7-12
120. Michael M.K., K. Hinson, K.H. Quesenberry, D.S. Wofford. 1996. Inheritance of resistance to the soybean looper in soybean. Crop Sci. 36(6): 1532-1537
121. Painter R.H. 1951. Insect Resistance In Crop Plants. Macmillan Co., New York
122. Raina A, Datta A. 1992. Molecular Cloning of a Gene Encoding a Seed-specfic Protein with Nutritionally Balanced Amino Acid Composition from Amaranthus. Proc Natl Acad Sci USA. 89:11774-11778
123. Rangappa M, M.E. Kraemer, P.S. Benepal. 1987. Pests. Soybean Genetics Newsletter. 14:227-228
124. Rector B.G., J.N. All, W.A. Parrott, H.R. Boerma. 1999. Quantitative trait loci for antixenosis resistance to corn earworm in soybean. Crop Sci., 39:531-538
125. Rector B.G., J.N. All, W.A. Parrott, H.R. Boerma. 2000. Quantitative trait loci for antibiosis resistance to corn earworm in soybean. Crop Sci. 40:233-238
126. Rector, B.G., J. N. All, W. A. Parrott and H. R. Boerma. 1998. Identification of molecular markers linked to quantitative trait loci for soybean resistance to Corn Earworm. Theor Appl Genet. 96:786-790
127. Rynolds G.W., C.M. Smiths. 1985. Effect of leaf position, leaf wounding and plant age of two soybean genotypes on soybean looper (Lepidoptera: Noctuidae) growth. Environ. Entomol. 14(4): 475-478
128. Shrivastava K.K., B.K. Srivastava, J.Y. Deole. 1988. Studies on chemical control and varital resistance of soybean against leaf-miner, Stopteryx subsecivella Zeller. Indian Journal of Plant Protection. 16(2): 147-151
129. Sisson V. A., P.A. Miller, W.V. Campbell, J.W. Van Duyn. 1976. Evidence of
inheritance of resistance to the Maxican been beetle in soybeans. Crop Sci. 16: 835-837
130. Smith C.M. 1985. Expression, mechanisms and chemistry of resistance in soybean, Glycine max L. (Merr.) to the soybean looper, Pseudoplusia includens (Walker). Insect Science and its Application. 6(3): 243-248
131. Smith C.M., N.H. Fischer. 1983. Chemical factors of an insect resistant soybean genotype affecting growth and survival of the soybean looper. Entomologia Experimentalis et Applicata. 33(3) : 343-345
132. Song S. I., C.H. Kim, S.J. Baek, Y. D. Choi. 1993. Nucleotide sequences of cDNAs encoding the precursors for soybean (Glycine max) trypsin inhibitor (Kunitz type). Plant Physiol. 101:1401-1402
133. Thakur N.S.A. 1988. Field screening of soybean cultivars against leaf rollers, Nacoleia sp. in Khasi hills of Meghalaya. Legume Research. 11(1): 44-46
134. Terry L. I., K. Chase, T. Jarvik, J. Orf, L. Mansur, K.G. Lark. 2000. Soybean quantitative trait loci for resistance to insects. Crop Sci. 40:375-382
135. Van Damme E J M, Barre A, Mazard A M, et al. 1999. Characterization and Molecualr Cloning of the Lectin from Helianthus Tuberosus. Eur J biochem. 259:135-142
136. Van Duyn J.W., S.G. Turnipseed, J.D. Maxwell. 1972. Resistance in soybeans to the Mexican bean beetle. Ⅱ. Reactions of the beetle to resistant plants. Crop Sci. 12: 561-562
137. Van Duyn J. W., S.G. Turnipseed, J.D. Maxwell. 1971. Resistance in Soybeans to the Mexican Bean Beetle Ⅰ: Source of Resistance. Crop Sci. 11:572-573
138. Yong N M, Johnston R A Z, Watson D C. 1991. The Amimo Acid Sequences of Jacalin and the Maclurapomifera Agglutinin. FEBS Letter. 282: 382-384
2．程林梅、孙毅、岳焕荣．2001．大豆生物工程研究进展．大豆科学20(1)：66-70
3．崔章林、盖钧镒．吉东风，任珍静，赵团结．1996．大豆种质资源对南京地区重要食叶性害虫抗性的鉴定．中国农业科学，29(4)：95-96
4．崔章林，盖钧镒，吉东风，任珍静．1997．大豆种质资源对食叶性害虫抗性的鉴定．大豆科学，16(2)：93-102
5．崔章林，盖钧镒，吉东风，任珍静．1997．南京地区大豆食叶性害虫种类调查与分析．大豆科学，16(1)：12-20
6．崔章林，盖钧镒．1996．大豆抗豆秆黑潜蝇研究进展．中国油料，18(3)：79-81
7．崔章林．盖钧镒．1996．大豆抗食叶性害虫研究进展．大豆科学，15(2)：149-158
8．崔章林，盖钧镒．1997．大豆种质资源对食叶性害虫抗性的鉴定．大豆科学，16(2)：93-102
9．崔章林．1995．南京地区大豆叶食性害虫重要种类分析与抗源鉴定．硕士学位论文，南京农业大学
10．邓九生，张大鹏，陈大明．2000．荔枝铜锌超氧物歧化酶基因的克隆与序列分析．广西农业生物科学．19(2)：73-76
11．董朝蓬，杜林方，段真2003．植物凝集素研究进展．天然产物研究与开发．15(1)：71-76
12．盖钧镒，崔章林．1997．大豆抗食叶性害虫育种鉴定方法与指标．作物学报，23(4)：400-407
13．盖钧镒，夏基康，崔章林，任珍静，浦奉华，吉东风．1989．我国南方大豆资源对豆秆黑潜蝇抗性的研究．大豆科学，8(2)：115-121
14．盖钧镒主编．2000．试验统计方法．北京：中国农业出版社
15．高文娜．2003．小麦抗锈病基冈分子标记．硕士学位论文．西农农业大学
16．高燕会，李润植，毛雪，李彩霞．2000．植物凝集素的防卫功能及其研究进展世界农业250(2)：33-35
17．高越峰，朱祯，肖桂芳，朱玉，吴茜，李向辉．1998．大豆Kunitz型胰蛋白酶抑制剂基因的分离及其在抗虫植物基因工程中的应用．植物学报40(5)：405-411
18．高越峰，朱祯，朱玉，肖桂芳，李向辉．1997．大豆Kunitz型胰蛋白酶抑制剂基因的克隆及其转基因烟草抗虫性初探．高技术通讯，(9)：5-9
19．郭凤英，沙广乐．1997．蛋白酶抑制剂研究进展．河南职技师院学报．25(4)：5-7
20．顾克余．1999．水稻汕优63及其亲本差异基因表达与克隆研究．博士学位论文，南京农业大学
21．郝峥嵘，刘庆法，季昀，叶鸣明，陈永青，沈大棱．1999．稻胚凝集素基因的克隆、序列分析及表达．西北植物学报．19(2)：183-189
22．J．萨姆布鲁克，D．W．拉塞尔．2002．分子克隆实验指南(第三版)．科学出版社
23．贾璇．2003．小麦抗叶锈基因Lr38的AFLP分子标记．硕士学位论文．河北农业大学
24．江树业，陈启锋，方宜钧，李维明，吴为人．2000．植物基因分离方法及其评述．福建农业大学学报．29(3)：261-268
25．李鸣．2003．AFLP技术在甘蔗品种鉴定和亲缘关系研究上的应用．硕士学位论文．广西大学
26．李玉英．2003．荞麦蛋白酶抑制剂基因的克隆及序列分析．硕士学位论文．山西大学
27．梁峰，常团结．2002．植物凝集素的研究进展．武汉大学学报．48(2)：232-238
28．柳武革，薛庆中．2000蛋白酶抑制剂及其在抗虫基因工程中的应用．生物技术通报．1：20-25
29．路子显，常团结．朱祯．2002．植物外源凝集素及其在植物基因工程中的应用．生物：工程进展．22(2)：3-9
30．南相玉，刘文萍，刘丽艳，吕晓波．何云霞，卫志明．1998．PEG介导BT基因转化大豆原生质体获转基因植株．大豆科学，17(4)：326-329
31．潘科，黄炳球，侯学文．2002．植物凝集素在病虫害防治中的研究进展．植物保护．28(4)：42-44
32．彭玉华．1996．大豆抗食叶性害虫研究概况．大豆通报，(1)：26
33．钱海丰，陈小荣，田振涛，薛庆中．2002蛋白酶抑制剂在抗虫基因工程中的应用．生命科学．14(5)：308-310
34．钦俊德著．1987．昆虫与植物的关系．北京：科学出版社
35．曲晓华，浦冠勤．2003．蛋白酶抑制剂的研究与应用．蚕桑茶叶通讯．1：19-22
36．苏军．蛋白酶抑制剂基因的杀虫机理及其应用．福建果树．100：19-21
37．孙祖东．盖钧镒．1999．大豆对食叶性害虫抗性的研究．中国农业科学，32(增刊)：81-88
38．孙祖东，盖钧镒．1999．大豆对食叶性害虫抗性机制的研究．中国油料作物学报，21(2)：60-64
39．孙祖东．盖钧镒．1999．大豆抗食叶性害虫遗传的初步研究．大豆科学．18(4)：300-304
40．孙祖东，盖钧镒．1999．大豆品种对斜纹夜蛾抗性机制初探．植物保护学报．26(4)：329-332
41．孙祖东，盖钧镒．2000．大豆抗斜纹夜蛾幼虫的遗传研究．作物学报，26(3)：341346
42．孙祖东，杨守臻，陈怀珠，韦德卫．2001．南宁大豆食叶性害虫调查．广西农业科学，(2)：104-106
43．孙祖东．1997．南京地区大豆对食叶性害虫抗性的鉴定、机制和遗传的研究．博士学位论文，南京农业大学
44．谈建中，楼程富，平野久．2000．大豆特殊种子蛋白豆类胰岛素基因的克隆与序列分析．浙江大学学报．26(1)107-110
45．王斌，翁曼丽．1996．AFLP的原理及其应用[J]．杂交水稻．(5)：27-30．
46．王春明，安井秀，古村醇，苏昌潮，翟虎渠，万建民．2003．水稻叶蝉抗性基因回交转育和CAPS标记辅助选择．中国农业科学．36(3)：237-241
47．王得元，李颖，黎振兴，黄爱兴．1998．DNA分子标记在作物杂种优势研究中的应用．西北农
业学报．7(3)：94-97
48．王慧．2003．大豆对食叶性害虫抗性的遗传及相关基因的SSR标记和定位的研究．硕士学位论文，南京农业大学
49．王玲．2002．辣椒遗传多样性的RAPD分析．硕士学位论文．福建农林大学
50．王伟，朱祯等．1999．双价抗虫基因陆地棉转化植株的获得．植物学报．41(4)：384-388
51．于新力，彭学贤．2001．香蕉果实成熟相关基因ACO1启动子区的克隆及其功能初探．生物工程学报．17(4)：428-431
52．王志斌，李学勇，郭三堆．1998．植物凝集素与抗虫基因工程．生物技术通报2：5-10
53．王白章．李杨瑞，张树珍等．2003．甘蔗ACC氧化酶基因片段的克隆与序列分析．遗传学报，30(1)：62-69
54．吴谡琦等．2001．分子标记技术的进展及其应用．高科技通讯，1：99-103．
55．吴业春．2003．大豆对食叶性害虫抗性鉴定及对斜纹夜蛾抗生性的遗传研究．硕士学位论文，南京农业大学
56．谢可方，董爱武，忻骅等．2002大豆KIJNIT7型胰蛋白酶抑制剂的稳定性及抗虫性研究．复旦学报．41(6)：631-634
57．徐春晓．2000．大豆对食叶性害虫抗性的植株反应和虫体反应及其在资源鉴定、遗传与选育中的应用．硕士学位论文，南京农业大学
58．徐香玲等．1997．Ti质粒介导的B．t．K—δ内毒素蛋白基因转化大豆的初步研究[J]．大豆科学，16(1)：9-11．
59．颜辉煌．1990．大豆对豆秆黑潜蝇抗选性、抗生性及耐虫性的一些研究．硕士学位论文，南京农业大学
60．杨辉霞，王芳，单雷，毕玉平．2003．花生球蛋白基因片段的克隆．花生学报．32(3)：15-18
61．杨素欣，冯献忠，刘平林，何玉科．2000．甘蓝中BcpLH同源基因的克隆及其序列分析．植物生理学报．26(4)363-368
62．伊佟明，黄敏仁．1997．AFLP分子标记及其在植物育种上的应用[J]．生物工程进展．17(1)：6-11．
63．余波澜，黄朝峰，周文娟，张文俊．2001．大麦1H特异性CAPs标记和ASA标记的创制．遗传学报28(6)：550-555
64．翟凤林等译(Maxwell F．G．等著)．1985．植物抗虫育种．北京：农业出版社
65．曾庆平，何振宇．1994．植物基因多态性的系统分类学研究(Ⅱ)凝集素基因的分子进化．长沙水电师院自然科学学报．9(3)：299-305
66．詹秋文，盖钧镒．1999．大豆资源对食叶性害虫抗性的差异反应及种质鉴定．安徽农业技术师范学院学报．13(4)：1-6
67．詹秋文．1999．大豆对食叶性害虫抗性植株反应的资源鉴定、遗传与选育．硕士学位论文，南京农业大学
68．詹秋文．2000．大豆种质资源对斜纹夜蛾(PRODENIA LITURA)抗性的鉴定．应用与环境生物学报．6(1)：18-23
69．张宝红．丰嵘编著．2000．棉花的抗虫性与抗虫棉．北京：中国农业科技出版社
70．张良佑，吴洪基等．1998．转基因水稻的抗虫性初探．华南农业大学学报．19(2)：4-7
71．张玉静．2000．分子遗传学．北京：科学出版社
72．张宗炳，曹骥主编．1990．害虫防治：策略与方法．北京：科学出版社
73．赵东，刘祖生．奚彪．2001．茶树多酚氧化酶基因的克隆及其序列比较．茶叶科学．21(2)：94-98
74．赵洪锟，李启云、王玉民，庄炳昌．2002．大豆Kunitz型胰蛋白酶抑制剂(SKTI)研究进展．大豆科学．21(3)：218-222
75．周明群编．1992．作物抗虫性原理及应用．北京：北京农业大学出版社
76．周国岭，杨光圣，傅延栋．2001．基因克隆技术．华中农业大学学报．20(6)：584-592
77．朱成松．顾和平，陈新．1998．大豆对食叶性害虫抗性的自然鉴定．江苏农业科学．2：33-35
78．朱成松．顾和平，陈新．2002．大豆抗虫基因工程研究进展．大豆通报．6：17-18
79．朱成松，张宝龙，王学军．1999．大豆对食叶性害虫的抗性与农艺性状的关系．中国油料作物学报，21(1)：59-62
80．祝水金，汪若海．1995．植物蛋白酶抑制因子及其在植物抗虫基因工程中的应用．生物技术．5(3)1-5
81．朱玉，吴茜，高越峰，徐鸿林，刘春明，周兆斓，朱祯，李向辉．1997．雪花莲外源凝集素基因的克隆、序列分析和植物表达载体的构建．农业生物技术学报。5(4)：331-338
82. Awadallah, W.H., M. F. Lufallah, E.M.A. El-Moneim, M.F. Ei-Metwally, M.Z. Hassan. 1990. Evaluation of some soybean for their resistance to the cotton leaf worm, Spodoptera littoralis boid. Agricultural Research Review, 68(1).
83. Beach, R.M., J.W. Todd, H.R. Boerma. 1988. Relative resistance of three soybean plant introductions to the soybean looper and velvebean caterpillar in Georgia. Journal of Entomological Science, 23(4):399-401.
84. Bostwick D E, Skaggs M I, Thompson G A. 1994.Organization and Characterization of Cururbita Phloem Lectin Genes. Plant Mol Boil. 26:887-897
85. Brier, H.B., D.J. Rogers. 1991. Leaf-feeding resistance to six Australian noctuids in soybean. Crop Protection, 10(4):320-324.
86. Burton, J.W., et al. 1986. Registration of insect resistant soybean germplasm lines N80-53201, N79-2282, N80-50232. Crop Science, 26(1)212.
87. Copper, R.L., et al. 1988. Registration of Mexican bean beetle resistant germplasm line HC83-1293. Crop Sci., 28:1037-1038.
88. Cooper R.L., R.B. Hammond. 1998. Registration of insect-resistant soybean germplasm lines HC95-24MB and HC95-15MB. Crop Sci. 39(2): 599
89. Prey K.J. 1981. Plant Breeding. Iowa State University Press
90. Gary, D. J., et al. 1985. Evaluation of soybean plant introductions for resistance to foliar feeding insects. Journal of the Mississippi Academy of Sciences, 30:67-82
91. Gai J.Y., J.K. Wang. 1998. Identification and estimation of a QTL model and its effects. Theor. Appl. Genet. 97:1162-1168
92. Gai J.Y., Z.D. Sun, Z.L. Cui. 1996. Studies on resistance mechanism of soybeans to leaf feeders. Soybean Genetics Newsletter. 1996, 25:55-56
93. Gary D.J., L. Lambert, J.D. Ouzts. 1985. Evaluation of soybean plant introductions for resistance to foliar feeding insects. Journal of the Mississippi Academy of Sciences. 30:67-82
94. Haq M., Karim ANMR, S. Alam. 1984. Preliminary study of varietal reaction of soybean cultivars to leaf defoliators. Tropical Grain Legume Bulletin. 29:35-37
95. Hartwig E.E., S.G. Turnipseed T.C. Kilen. 1984. Registration of soybean germplasm line D75-10169. Crop Sci. 24:214-215
96. Hartwig, E.E., and T.C.K. 1990. Registration of soybean cultivar Lamar. Crop Sci., 30(1):231.
97. Hilder V.A, Gatehouse A.M.R, Sheerman S.E. 1987. Nature. 330:160-165
98. Hilder V.A. Powell K.S. Gatehouse A.W.R. et al. 1995. Expression of Snowdrop Lectin in Transgenic Tobacco Plants Results in Added Protection Against Aphids. Transgenic Research. 4:18-25
99. Hopins J.D. et al. 1983. Distribution of bean pod mottle virus in Arkansas soybean as related to the bean leaf beetle, Cerotoma trifurcata, (Coleoptera: Chrysomelidae) Population. Environ. Entomol. 12(5): 1564-1567
100. Jivaid I., J.M. Joshi, R.B. Dadson, M. Nobaakht. 1991. Leaf feeding resistance in soybean breeding lines to corn earworm (Heliothis zes Boddie). Soybean Genetics Newsletter. 18:271-274
101. Jofuku K.D., RB. Goldberg. 1989. Kunitz trypsin inhibitor genes are differentially expressed during the soybean life cycle and in transformed tobacco plants. Plant Cell. 1(11): 1079-1093
102. Johnson R. et al. 1989. Proc natl Acad Sci USA. 86:9871
103. Joudrier P.E., D.E. Foard, L.A. Floener, B.A. Larkins. 1987. Isolationand sequence of cDNA encoding the soybean protease inhibitors PI Ⅵ and C-Ⅱ. Plant Mol. Biol. 10:35-42
104. Kilen T.C., J.H. Hatchett, E.E. Hartwig. 1977. Evaluation of early generation soybeans for resistance to soybean looper. Crop Sci. 17:397-398
105. Kilen T.C., L. Lambert. 1986. Evidence for different genes controlling insect resistance in three soybean genotypes. Crop Sci. 26(6): 869-871
106. Kilen T.C., L. Lambert. 1993. Registration of three glabrous and three pubescent soybean germplasm lines susceptible (D88-5320, D88-5295), moderately resistant (D88-5328, D88-5272) or resistant(D90-9216, D90-9220) to foliar-feeding insects. Crop Sci. 33(1): 215
107. Kilen T.C., L. Lambert. 1998. Genetic control of insect resistance in soybean germplasm PI 417061. Crop Sci. 38:652-654
108. Konami Y,Yamamoto K, Osawa T. 1991. The Primary Structures of two Types of the Ulex Europeus Seed Lectin. Jounal of Biochemistry. 109:650-658
109. Kraemer M.E., M. Rangappa, W. Gade, P.S. Benepal. 1987. Induction of tripsin inhibitors in soybean leaves by Mexican bean beetle (Coleoptera: Coccinellidae) defoliation. J. Econ. Entomol. 80(1): 237-241
110. K S Powell, A M R Gatehouse, et al. 1995. Antifeedant effects of plant lectins and enzyse on the adult stage of the rice brown plant hopper, Nilaparvata lugens. Entomologia Experimentalis et Applicatan. 75:51
111. Lambert L., L.G. Heatherly. 1991. Soil water potential effects on soybean looper feeding on soybean leaves. Crop Sci. 31(6): 1625-1628
112. Lambert L., R.M. Beach, T.C. Kilen. 1992. Soybean pubescence and it's influence on larval development and oviposition preference of Lepidopterous insects. Crop Sci. 32:463-466
113. Lambert L., T.C. Kilen. 1984. Influence of three soybean plant genotypes and their F, intercrosses on the development of five insect species. J. of Econ. Entomol. 77(3): 622-625
114. Lambert L., T.C. Kilen. 1984. Insect resistance factor in soybean PI's 229358 and 227687 demonstrated by drafting. Crop Sci. 24:163-165
115. Lambert L., T.C. Kilen. 1984. Multiple Insect resistance in several soybean genotypes.
Crop Sci. 24:887-890
116. Lin H, M. Kogan, D. Fischer. 1990. Induced resistance in soybean to the Mexican been beetle (Coleoptera: Coccinellidae): comparisons of inducing factors. Environmental Entomology. 19(6): 1852-1857
117. Luedders V.D., W.A. Dickerson. 1977. Resistance of selected genotypes and segregating populations to cabbage looper feeding. Crop Sci. 17:395-396
118. Mbrahtu T. 1986. Efficiency of different selection and screening methods to identify productive soybean breeding lines resistant to Mexican bean beetle. Dissertation Abstracts International. 47(5): 1800
119. Mrahtu T., W.J. Kenworthy, T.C. Elden. 1990. Genetic study of resistance to Mexican bean beetle in soybean lines. Journal of Genetics and Breeding. 44(1) :7-12
120. Michael M.K., K. Hinson, K.H. Quesenberry, D.S. Wofford. 1996. Inheritance of resistance to the soybean looper in soybean. Crop Sci. 36(6): 1532-1537
121. Painter R.H. 1951. Insect Resistance In Crop Plants. Macmillan Co., New York
122. Raina A, Datta A. 1992. Molecular Cloning of a Gene Encoding a Seed-specfic Protein with Nutritionally Balanced Amino Acid Composition from Amaranthus. Proc Natl Acad Sci USA. 89:11774-11778
123. Rangappa M, M.E. Kraemer, P.S. Benepal. 1987. Pests. Soybean Genetics Newsletter. 14:227-228
124. Rector B.G., J.N. All, W.A. Parrott, H.R. Boerma. 1999. Quantitative trait loci for antixenosis resistance to corn earworm in soybean. Crop Sci., 39:531-538
125. Rector B.G., J.N. All, W.A. Parrott, H.R. Boerma. 2000. Quantitative trait loci for antibiosis resistance to corn earworm in soybean. Crop Sci. 40:233-238
126. Rector, B.G., J. N. All, W. A. Parrott and H. R. Boerma. 1998. Identification of molecular markers linked to quantitative trait loci for soybean resistance to Corn Earworm. Theor Appl Genet. 96:786-790
127. Rynolds G.W., C.M. Smiths. 1985. Effect of leaf position, leaf wounding and plant age of two soybean genotypes on soybean looper (Lepidoptera: Noctuidae) growth. Environ. Entomol. 14(4): 475-478
128. Shrivastava K.K., B.K. Srivastava, J.Y. Deole. 1988. Studies on chemical control and varital resistance of soybean against leaf-miner, Stopteryx subsecivella Zeller. Indian Journal of Plant Protection. 16(2): 147-151
129. Sisson V. A., P.A. Miller, W.V. Campbell, J.W. Van Duyn. 1976. Evidence of
inheritance of resistance to the Maxican been beetle in soybeans. Crop Sci. 16: 835-837
130. Smith C.M. 1985. Expression, mechanisms and chemistry of resistance in soybean, Glycine max L. (Merr.) to the soybean looper, Pseudoplusia includens (Walker). Insect Science and its Application. 6(3): 243-248
131. Smith C.M., N.H. Fischer. 1983. Chemical factors of an insect resistant soybean genotype affecting growth and survival of the soybean looper. Entomologia Experimentalis et Applicata. 33(3) : 343-345
132. Song S. I., C.H. Kim, S.J. Baek, Y. D. Choi. 1993. Nucleotide sequences of cDNAs encoding the precursors for soybean (Glycine max) trypsin inhibitor (Kunitz type). Plant Physiol. 101:1401-1402
133. Thakur N.S.A. 1988. Field screening of soybean cultivars against leaf rollers, Nacoleia sp. in Khasi hills of Meghalaya. Legume Research. 11(1): 44-46
134. Terry L. I., K. Chase, T. Jarvik, J. Orf, L. Mansur, K.G. Lark. 2000. Soybean quantitative trait loci for resistance to insects. Crop Sci. 40:375-382
135. Van Damme E J M, Barre A, Mazard A M, et al. 1999. Characterization and Molecualr Cloning of the Lectin from Helianthus Tuberosus. Eur J biochem. 259:135-142
136. Van Duyn J.W., S.G. Turnipseed, J.D. Maxwell. 1972. Resistance in soybeans to the Mexican bean beetle. Ⅱ. Reactions of the beetle to resistant plants. Crop Sci. 12: 561-562
137. Van Duyn J. W., S.G. Turnipseed, J.D. Maxwell. 1971. Resistance in Soybeans to the Mexican Bean Beetle Ⅰ: Source of Resistance. Crop Sci. 11:572-573
138. Yong N M, Johnston R A Z, Watson D C. 1991. The Amimo Acid Sequences of Jacalin and the Maclurapomifera Agglutinin. FEBS Letter. 282: 382-384