电针不同部位穴位减肥效应及机制差异的实验研究
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摘要
研究目的：
观察电针不同穴位：后三里、曲池、天枢、耳穴(“胃”耳轮脚消失处和“饥点”耳屏外侧面中点)治疗食源性肥胖的疗效、对下丘脑pAMPK α、GK、Glut-3表达,胰腺中pAMPKα、GK表达及血清中内脂素蛋白含量表达的影响,探讨不同穴位减肥的效应差异及中枢机制,为临床治疗食源性肥胖的选穴提供参考依据。
研究方法：
通过高脂饮食制作食源性肥胖大鼠模型,造模成功后,实验动物分为正常组、肥胖模型组、电针治疗组(三里组、曲池组、天枢组、耳穴组)。每日治疗一次、称量体重,6天休息1日,共5周。治疗结束后,运用免疫组化观察各组下丘脑及胰腺中的pAMPK α、GK、Glut-3的表达情况,运用酶联免疫法测定血清内脂素蛋白的含量。
实验结果：
治疗结束后,电针治疗组体重均明显减少(p=0.012),说明电针后三里、曲池、天枢、耳穴均能有效降低体重和脂肪含量,曲池、天枢穴减肥效应最为明显(p<0.01),曲池穴减脂效应较其它三组更为突出(p=0.001)；食源性肥胖大鼠下丘脑Glut-3表达下调,电针治疗各组大鼠下丘脑Glut-3表达较模型组升高；食源性肥胖大鼠下丘脑及胰腺中GK表达下调,电针治疗组下丘脑及胰腺中GK表达较模型组升高。造模及电针治疗对大鼠下丘脑中pAMPKα的表达均无影响,但食源性肥胖大鼠胰腺中的pAMPK α表达下调,电针后三里和曲池穴可使胰腺中pAMPK α表达上调,天枢及耳穴对pAMPK α的影响则无统计学意义；在对血清中内脂素的调节上,只有后三里一穴能使上调的蛋白含量降低(P<0.01),而其它三组穴位无此作用。
结论：
在电针治疗食源性肥胖病上,穴位具有特异性,不同的穴位在对下丘脑及外周肥胖因子的调节上表现出了不同的作用,据以上实验结果可推论：曲池与天枢穴具有减重与减脂双重功效,足三里作为针灸中的保健穴在减肥上效应并非很突出,耳穴则更侧重于减脂,能有效的抑制旺盛的食欲,但在改善机体能量代谢上作用较小,这可能也是临床上多采用耳穴与体针相结合的方式治疗食源性肥胖的原因。
Objective:
To observe the effects of electro-acupuncture (EA) at ST36,LI11,ST25and auricular acupoint(stomach point in ending of helix crus,Jidian in midpoint of tragus lateral surface) treating diet-induced obesity (DIO) rats, and influence on expression of phosphoric-AMP-activated-protein-Kinase-a(pAMPKa), Glucokinase(GK),Glut-3in hypothalamus and pancreas and expression of serum visfatin protein,to explore the effect characteristics and the possible central mechanisms of acupuncture at different acupoints in treating simple obesity,providing evidence for clinical acupoint selection in treating DIO.
Method:
To establish DIO rat model by feeding with high fat diet. Rats were randomly divided into3groups:(1) normal control group,(2) DIO model group (3)EA group(subdivided into ST36,LI11,ST25and auricular acupoint group).Treatments and body mass weighing were administered everyday,resting one day per week,with5-weeks treatment course.After treatments, expression of pAMPKa,GK and Glut-3in hypothalamus and pancreas were mersured by immunohistochemistry method, serum visfatin protein level was determined by ELISA method.
Result:
After treatment, rats body weight in EA group reduced significantly(p=0.012),which indicated that EA at ST36,LI11,ST25and auricular acupoint can all reduce body weight and fat content,with LI11and ST25most effective(p<0.01) and LI11better than the other three groups in reducing fat content.In DIO model group,expression of Glut-3in hypothalamus decreased,and it increased after EA treatment in each group. In DIO model group,expression of GK in hypothalamus and pancreas decreased,it also increased after EA treatment in each group. There was no difference in expression of pAMPKa in hypothalamus both in model group and EA group.However, expression of pAMPKa in pancreas of DIO rats reduced,and it both increased after EA treatment at ST36and LI11,while no statistic significance in EA at ST25and auricular acupoint.In the aspect of serum visfatin,EA at ST36can only decrease the serum visfatin protein(P<0.01),and this effect didn't present in the other three acupoints groups.
Conclusion:
There was specificity in acupoint of EA treating DIO.The regulation of hypothalamus and pancreas obesity factors in different acupoints show different effects.According to experimental. results above,EA at LI11and ST25can both reduce body weight and fat content,and as an health-care acupoint,ST36may not presente an outstanding effect in treating obesity,while auricular acupoint was better at reducing fat content and inhibiting vigorous appetide,yet limited in improving energy metabolism condition,which accounts for the reason why auricular acupoints combined with body acupoints was usually applied to treat DIO in clinic.
观察电针不同穴位：后三里、曲池、天枢、耳穴(“胃”耳轮脚消失处和“饥点”耳屏外侧面中点)治疗食源性肥胖的疗效、对下丘脑pAMPK α、GK、Glut-3表达,胰腺中pAMPKα、GK表达及血清中内脂素蛋白含量表达的影响,探讨不同穴位减肥的效应差异及中枢机制,为临床治疗食源性肥胖的选穴提供参考依据。
研究方法：
通过高脂饮食制作食源性肥胖大鼠模型,造模成功后,实验动物分为正常组、肥胖模型组、电针治疗组(三里组、曲池组、天枢组、耳穴组)。每日治疗一次、称量体重,6天休息1日,共5周。治疗结束后,运用免疫组化观察各组下丘脑及胰腺中的pAMPK α、GK、Glut-3的表达情况,运用酶联免疫法测定血清内脂素蛋白的含量。
实验结果：
治疗结束后,电针治疗组体重均明显减少(p=0.012),说明电针后三里、曲池、天枢、耳穴均能有效降低体重和脂肪含量,曲池、天枢穴减肥效应最为明显(p<0.01),曲池穴减脂效应较其它三组更为突出(p=0.001)；食源性肥胖大鼠下丘脑Glut-3表达下调,电针治疗各组大鼠下丘脑Glut-3表达较模型组升高；食源性肥胖大鼠下丘脑及胰腺中GK表达下调,电针治疗组下丘脑及胰腺中GK表达较模型组升高。造模及电针治疗对大鼠下丘脑中pAMPKα的表达均无影响,但食源性肥胖大鼠胰腺中的pAMPK α表达下调,电针后三里和曲池穴可使胰腺中pAMPK α表达上调,天枢及耳穴对pAMPK α的影响则无统计学意义；在对血清中内脂素的调节上,只有后三里一穴能使上调的蛋白含量降低(P<0.01),而其它三组穴位无此作用。
结论：
在电针治疗食源性肥胖病上,穴位具有特异性,不同的穴位在对下丘脑及外周肥胖因子的调节上表现出了不同的作用,据以上实验结果可推论：曲池与天枢穴具有减重与减脂双重功效,足三里作为针灸中的保健穴在减肥上效应并非很突出,耳穴则更侧重于减脂,能有效的抑制旺盛的食欲,但在改善机体能量代谢上作用较小,这可能也是临床上多采用耳穴与体针相结合的方式治疗食源性肥胖的原因。
Objective:
To observe the effects of electro-acupuncture (EA) at ST36,LI11,ST25and auricular acupoint(stomach point in ending of helix crus,Jidian in midpoint of tragus lateral surface) treating diet-induced obesity (DIO) rats, and influence on expression of phosphoric-AMP-activated-protein-Kinase-a(pAMPKa), Glucokinase(GK),Glut-3in hypothalamus and pancreas and expression of serum visfatin protein,to explore the effect characteristics and the possible central mechanisms of acupuncture at different acupoints in treating simple obesity,providing evidence for clinical acupoint selection in treating DIO.
Method:
To establish DIO rat model by feeding with high fat diet. Rats were randomly divided into3groups:(1) normal control group,(2) DIO model group (3)EA group(subdivided into ST36,LI11,ST25and auricular acupoint group).Treatments and body mass weighing were administered everyday,resting one day per week,with5-weeks treatment course.After treatments, expression of pAMPKa,GK and Glut-3in hypothalamus and pancreas were mersured by immunohistochemistry method, serum visfatin protein level was determined by ELISA method.
Result:
After treatment, rats body weight in EA group reduced significantly(p=0.012),which indicated that EA at ST36,LI11,ST25and auricular acupoint can all reduce body weight and fat content,with LI11and ST25most effective(p<0.01) and LI11better than the other three groups in reducing fat content.In DIO model group,expression of Glut-3in hypothalamus decreased,and it increased after EA treatment in each group. In DIO model group,expression of GK in hypothalamus and pancreas decreased,it also increased after EA treatment in each group. There was no difference in expression of pAMPKa in hypothalamus both in model group and EA group.However, expression of pAMPKa in pancreas of DIO rats reduced,and it both increased after EA treatment at ST36and LI11,while no statistic significance in EA at ST25and auricular acupoint.In the aspect of serum visfatin,EA at ST36can only decrease the serum visfatin protein(P<0.01),and this effect didn't present in the other three acupoints groups.
Conclusion:
There was specificity in acupoint of EA treating DIO.The regulation of hypothalamus and pancreas obesity factors in different acupoints show different effects.According to experimental. results above,EA at LI11and ST25can both reduce body weight and fat content,and as an health-care acupoint,ST36may not presente an outstanding effect in treating obesity,while auricular acupoint was better at reducing fat content and inhibiting vigorous appetide,yet limited in improving energy metabolism condition,which accounts for the reason why auricular acupoints combined with body acupoints was usually applied to treat DIO in clinic.
引文
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