摘要
随着石油工业的迅速发展,土壤石油污染日益严重,但相应的治理技术相对落后。当前,石油污染土壤生物修复技术研究成为保障石油污染土地生态恢复重建的关键。为了给石油污染土壤生物修复提供技术支持和理论依据,课题针对石油污染土壤的现状以及石油污染土壤生物修复存在的主要问题,在污染生态学基础理论的指导下,以中原油田石油污染土壤和土著生物为研究对象开展石油污染土壤生物修复研究工作。
通过人工配制石油污染土壤盆栽试验研究石油污染土壤对植物种子发芽率、株高和鲜重的影响,筛选具有生物修复研究潜力的植物。以原油为唯一的碳源和营养从中原油田石油污染土壤中筛选石油降解微生物。通过人工石油污染土壤盆钵修复盆栽试验研究验证微生物、植物及植物——微生物组合的石油降解效果,并研究环境因素(土壤含水量、土壤含盐量、秸杆添加量等)对石油污染土壤生物修复的影响。利用中原油田石油污染土壤,通过原位修复盆钵模拟试验,考察狗牙根加微生物组合、棉花加微生物组合、狗牙根与棉花加微生物组合对环境的适应性及降解效果。研究取得的主要结果如下:
(1)向日葵、棉花、高丹草、狗牙根在0—45g/kg石油污染水平范围内发芽率、株高及鲜重受影响较小,能生长在石油污染的土壤上,并且它们对土壤中石油降解也具有一定的效果。其中狗牙根,生存能力强,是试验中的最具有修复土壤石油污染研究潜力的植物。
(2)从中原油田采油区石油污染土壤中获得的微生物对土壤石油污染具有显著修复效果的微生物,添加微生物120天后,石油去除率可达41.6%;自然条件下向日葵、狗牙根、棉花、高丹草均能与土著微生物共生进行土壤石油污染生物修复。其中,经过120天后,棉花与微生物群联合修复可以使石油降解率达到85.67%,超过它们单独修复的去除率之和,在四种组合中对污染土壤中石油的降解效果最好。
(3)环境因素对石油污染土壤生物修复有着重要影响。一般来说,随着土壤含盐量的增加,土壤石油污染生物降解速率减慢:有利于生物修复土壤中石油污染物的土壤相对湿度为60%—80%;土壤中秸杆添加量为20g/kg时生物对土壤中石油污染物的生物修复效果较好。同时,土壤良好的通气条件、将有利于生物活性的保持,加速石油的降解。
(4)在中原油田石油污染土壤原位生物修复模拟试验中,在120天的修复时间内,狗牙根与棉花加菌组合的石油降解率达到56.38%,是狗牙根加微生物组合、棉花加微生物组合组合、狗牙根与棉花加微生物组合中效果最好的一组。
With rapid development of petroleum industry, soil contaminated condition become more and moer serious.But the purification technology and bioremediation process can not match the environmental requirement. Currently,research on Bioremediation of petroleum contaminated soil become a key factor to ensure the rehabilitation of petroleum contaminated soil . In order to provide both theoretic parameters and practical techniques for phytoremediation of petroleum contaminated soil, According to the actual state of petroleum contaminated soil and main problems in bioremediation of petroleum contaminated soil, under guidance of the pollution ecology theory,Taking petroleum contaminated soil of Zhongyuan Oil Field、native plants and indigenous microorganisms as the research object, bioremediation of petroleum contaminated soil was was studied.
Studying the effect of artificial oil-contaminated soil on the germination and growth of plant seedling by the pot-planting experiment screen plants with experimental potential to phytoremediation.Taking petroleum as the unique Source of carbon and nutrition, the microorganisms which degradated the petroleum were screened from the petroleum contaminated soil which was collected from Zhongyuan oil field .petroleum and uncontamined Fluvo-aquic soil around Zhongyuan Oil Field are mixed together in different ratio to test oil degrading effects of microorganisms, plants and plants combined with microorganisms and study effects of environmental factors (total salt content, water content, straw etc.)on the bioremediation of petroleum contaminated soil by pot experiments.the in-situ remediation pot simulation experiment of petroleum contaminated soil which collected from Zhongyuan oil field was conducted to investigate environmental adaptability and oil degrading effects of microorganisms combined with cotton and Bermuda grass, microorganisms combined with cotton , microorganisms combined with Bermuda grass.The main results are as follows:
(1) The sprouting ratio, height and fresh weight of sunflower, cotton, Bermuda grass, sorghum sudanense have no significantly change with the concentrations of soil oil from 0 to 45 g/kg. They could grow on oil contaminated soil, and They can help to rehabilitate the polluted soil. Bermuda grass can be fit to grow on the crude oil-contaminated soils by itself, it is a plant with a biggest experimental potential to phytoremediation oil-contaminated in this experiment. (2) The micro-organism which can obviousiy rehabilitate the petroleum-polluted soil exist in oil contaminated soil of Oil Extraction Area of Zhongyuan Oil Field. They was added to the soil, 120 days later, 67.0% pollution was removed.The plants , such as sunflower, cotton, Bermuda grass, sorghum sudanense can naturally grow with native micro-organism to rehabilitate soil.The cotton combined with microorganisms got the best effect in four groups and the degradation rate of petroleum was 85.67% after 120d. more than the sum of their individual ability. (3) Environmental factors have important influence on Bioremediation of Petroleum Polluted Soil.Commonly,with the addition of salt in the soil, removed speed of petroleum slow down. The favorable soil moisture to Bioremediation of petroleum contaminated soil was 65 to 75 percent of field capacity.The optimum straw content in the soil was 20g/kg for the Bioremediation of petroleum contaminated soil. And meanwhile it is ecessary to keep soil under proper condition, suchas good ventilation. All these were helpful to accelerate the decomposition rate of pollutant. (4) In the simulation test of Bioremediation of Petroleum Polluted Soil in the Oil Extraction Area of Zhongyuan Oil Field, The microorganisms combined with cotton and Bermuda grass got the best effect in all the treatments and the degradation rate of petroleum was 56.38% after 120d.
引文
[1]张兴儒,张士权.油气田开发建设与环境影响[M].北京石油工业出版社,1998:1-190.
[2]何良菊,魏德洲,张维庆.土壤微生物处理石油污染的研究[J].环境科学进展,1999,7(3):110-115.
[3]陆秀君,郭书海,孙清,等.石油污染土壤的修复技术研究现状及展望[J].沈阳农业大学学报,2003,34(1):63-67.
[4]蔺听,李培军,台培东等.石油污染土壤植物一微生物修复研究进展[J].生态学杂,2006,25(1):93-100.
[5]丁克强,孙铁珩,李培军.石油污染土壤的生物修复技术[J].生态学杂志,2000,19(2):50-55.
[6]陈鹤建.原油在土壤中的渗透及降解规律[J].油气田环境保护,2000,10(4):14-15.
[7]吕志萍,程龙飞.石油污染土壤中石油含量对玉米的影响[J].油气田环境保护,2001,11(1):36-37.
[8]孙铁珩,周启星,李培军.污染生态学[M].北京:科学出版社,2001:309-368.
[9]卢丽丽,石辉.植物修复石油污染土壤的研究进展[J].化工环保,2007,27(3):245-249.
[10]周启星,宋玉芳,等.污染土壤修复原理与方法[M].北京:科学出版社,2004:22-42.
[11]Carraway,J.W.and Doyle,J.R.Innovative remedial action at awood-treeting superfund site[J].Tappi Journnal,1991,174(1):113-118.
[12]孙清,陆秀君,梁成华.土壤的石油污染研究进展[J].沈阳农业大学学报,2002,33(5):390-393.
[13]魏树和,周启星,Pavel V.Koval,等.有机污染环境植物修复技术[J].生态学杂志,2006,25(6):716-721.
[14]陈晓东,常文越,邵春岩.土壤污染生物修复技术研究进展[J].环境保护科学,2001,27(107):21-25.
[15]王一华,傅荣恕.中国生物修复的应用及进展[J].山东师范大学学报(自然科学版),2003,18(2):79-83.
[16]马文漪,杨柳燕.环境生物工程[M].南京:南京大学出版社,1998.pp250
[17]唐世荣,黄昌勇,朱祖祥.利用植物修复污染土壤研究进展[J].环境科学进展,1996,4(6):10-16.
[18]易筱筠,党志,石林.有机污染物污染土壤的植物修复.农业环境保护[J].2002,2l(5):477-479.
[19]A lkorta I,Garbisu C.Phytoremediation of organic contam inants in soils[J].Bioresour Technol,2001,79(3):273-276
[20]方晰,田大伦,康文星.湘潭锰矿矿渣废弃地植被修复盆栽试验[J].中南林业科技大学学报(自然科学版),2007,27(1):14-19.
[21]Li X,Feng Y,Sawatsky N.Importance of soil water relations in assessing the endpoint of bioremediated soils:Ⅰ.Plant growth[J].Plant and Soil,1997,192:219-226.
[22]张松林,董庆士,周喜滨,等.人为石油污染土壤紫花苜蓿田间修复试验[J].兰州大学学报(自然科学版),2008,44(1):47-50.
[23]李方敏,姚金龙,王琼山.修复石油污染土壤的植物筛选[J].中国农学通报,2006,22(9):429-431.
[24]欧阳威,刘红,于勇勇等.高羊茅对微生物强化修复石油污染土壤影响的研究[J].环境污染治理技术与设备,2006,7(1):94-97.
[25]ANDREOTTI G,PLATA N,PORTA A,et al.Phytoremediation of hydrocarbons-polluted agricultural soils[J].Phytoremediation,Wetlands,and Sediments,2001,6(5):41-51.
[26]KUCEROVA P,WIESCHE C I N,WOLTERr M,et al.The ability of different plant species to remove polycyclic aromatic hydrocarbons and polychlorinated biphenyls from incubation media[J].Biotechnology Letters,2001,23(16):1355-1359.
[27]SPRIGGS T,BANKS M K,SCHWAB P.Phytoremediation of polycyclic aromatic hydrocarbons in manufactured gas plant-impacted soil[J].Journal of Environmental Quality,2005,34:1755-1762.
[28]程国玲,李培军,王风友,等.多环芳烃污染土壤的植物与微生物修复研究进展[J].环境污染治理技术与设备,2003,4(6):30-36.
[29]PARRISH Z D,BANKS M K,SCHWAB A P.Effect of root death and decay on dissipation of polycyclic aromatic hydrocarbons in the rhizosphere of yellow sweet clover and tall rescue[J].Journal of Environmental quality,2005,34:207-216.
[30]WILTSE C C,ROONEY W L,CHEN Z,et al.Greenhouse evaluation of agronomic and crude oil-phytoremediation potential among alfalfa genotypes[J].Journal of Environmental Quality,1998,27:169-173.
[31]Roy S,Labelle S,Mehta P,et al.Phytoremediation of heavy metal and PAH-contam inated brownfield sites[J].Plant Soil,2005,272(1-2):277-290.
[32]丁克强,骆永明.苜蓿修复重金属Cu和有机物苯并[a]芘复合污染土壤的研究[J].农业环境科学学报,2005,24(4):766-770.
[33]CAMPBELL S,PAQUIN D,AWAYA J D,et al.Remediation of benzo[a]pyrene and chrysene-contaminated soil with industrial hemp(Cannabis sativa)[J].International Journal of Phytoremediation,2002,4(2):157-168.
[34]PAQUIN D,OGOSHI R,CAMPBELL S,et al.Bench-scale phyoremediation of polycyclic aromatic hydrocarbon-contaminated marine sediment with tropical plants[J].International Journal of Phytoremediation,2002,4(4):297-313.
[35]WIDDOWSON M A,SHEARER S,ANDERSEN R G,et al.Remediation of polycyclic aromatic hydrocarbon compounds in grounderwater using poplar trees[J].Environmental Science & Technology,2005,39:1598-1605.
[36]XU S Y,CHEN Y X,WUW X,et al.Enhanced dissipation of phenenthrene and pyrene in spiked soils by combined plants cultivation[J].Science of the Total Environment,2006,363:206-215.
[37]桑伟莲,孔繁翔.植物修复研究进展[J].环境科学进展,1999,7(3):40-44.
[38]LISTE H H,ALEXANDER M.Rapid screening of plants promoting phenanthrene degradation [J].Journal of Environmental Quality,1999,28:1376-1377.
[39]Wild E,Dent J,Thomas G O,et al.Direct observation of organic contaminant uptake,storage,and metabolism within plant roots[J].Environmental Science & Technology,2005,39:3695-3702.
[40]GAO Y Z,ZHU L Z.Plant uptake,accumulation and translocation of phenanthrene and pyrene in soils[J].Chemosphere,2004,55:1169-1178.
[41]FISMES J,PERRIN-GANIER C,EMPEREUR-BISSONNET P,et al.Soil-to-root transfer and translocation of polycyclic aromatic hydrocarbons by vegetable growth on industrial contaminated soils[J].Journal of Environmental Quality,2002,31:1649-1656.
[42]WILD S R,BERROW M L,MCGRATH S P,et al.Polynuclear aromatic hydrocarbons in crops from long term sewage amended field experiments[J].Environmental Pollution.1992,76:23-31.
[43]BANKS M K,LEE E,SCHWAB A P.Evaluation of dissipation mechanisms for benzo[a]pyrene in the rhizosphere of tall fescue[J].Journal of Environmental Quality,1999,28:294-298.
[44]REILLEY K A,BANKS M K,SCHWAB A P.Dissipation of polycyclic aromatic hydrocarbons in the rhizosphere[J].Journal of Environmental Quality,1996,25:212-219.
[45]凌婉婷,朱利中,高彦征等.植物根对土壤中多环芳烃的吸收及预测[J].生态学报,2005,25(9):2320-2325.
[46]焦杏春,陈素华,沈伟然等.水稻根系对多环芳烃的吸着与吸收[J].环境科学,2006,27(4):760-764.
[47]郜红建,蒋新,常江等.根分泌物在污染土壤生物修复中的作用[J].生态学杂志.2004,23(4):135-139.
[48]APRILL W,SIMS R C.Evaluation of the use of prairie grasses for stimulating polycyclic aromatic hydrocarbon treatment in soil[J].Chemosphere,1990,20:253-265.
[49]LISTE H H,FELGENTREU D.Crop growth,culturable bacteria,and degradation of petrolhydrocarbons(PHCs) in a long-term contaminated field soil[J].Applied Soil Ecology,2006,31:43-52.
[50]KRUTZ L J,BEYROUTY C A,GENTRY T J,et al.Selective enrichment of a pyrene degrader population and enhanced pyrene degradation in Bermuda grass rhizosphere[J].Biology and Fertility of Soils,2005,41(5):359-364.
[51]LING W T,GAO Y Z.Promoted dissipation of phenanthrene and pyrene in soils by amaranth (Amaranthus tricolor L.)[J].Environmental Geology,2004,46(5):553-560.
[52]MAILA M P,RANDIMA P,CLOETE T E.Multispecies and monoculture rhizoremediation of polycyclic aromatic hydrocarbons(PAHs) from the soil[J].International Journal of Phytoremediation,2005,7(2):87-98.
[53]HUANG X D,EI-ALAWI Y,PENROSE D M,et al.Responses of three grass species to creosote during phytoremediation[M].Environmental Pollution,2004,453-463.
[54]Kechavarzi C,Pettersson K,Leeds-Harrison P,et al.Root establishment of perennial ryegrass(L.perenne) indiesel contaminated subsurface soil layers[J].Environ Pollut,2007,145(1):68-74.
[55]CORGIE S C,JONER E J,LEYVAL C.Rhizospheric degradation of phenanthrene is a function of proximity to roots[J].Plant and Soil,2003,257(1):143-150..
[56]Merkl N,Schultze-kraft R,Infante C.Phytorem ediation in the tropics-influence of heavy crude oil on rootmorphological characteristics of graminoids[J].Environ.Pollut.,2005,138(1):86-91.
[57]LISTE H H,FELGENTREU D.Crop growth,culturable bacteria,and degradation of petrolhydrocarbons(PHCs) in a long-term contaminated field soil[J].Applied Soil Ecology,2006,31(1-2):43-52.
[58]陈嫣,李广贺,张旭等.石油污染土壤植物根际微生态环境与降解效应[J].清华大学学报(自然科学版),2005,45(6):784-787.
[59]Kirk J L,Klironomos J N,Lee H,e t a 1.The effects of perennialryegrass and alfalfa on microbial abundance and diversity in petroleum contaminated soil[J]..Environ Pollut,2005,133(3):455-465.
[60]SICILIANO S D,GERMIDA J J,SIClLIANO S D.Biology analysis and fatty acid methyl ester profiles indicate that pseudomonad inoculants that promote phytoremediation alter the root-associated microbial community of Bromus biebersteinii[J].Soil Biology & Biochemistry,1998,30:1717-1723.
[61]丁克强,骆永明,刘世亮,等.黑麦草对菲污染土壤修复的初步研究[J].土壤,2002,34(4):233-236.
[62]丁克强,骆永明,刘世亮,等.黑麦草对土壤中苯并[a]芘动态变化的影响[J].土壤学报,2004,41(3):348-353.
[63]LIU S L,LUO Y M,CAO Z H,et al.Degradation of benzo[a]pyrene in soil with arbuscular mycorrhizal alfalfa[J].Environmental Geochemistry and Health,2004,26(2):285-293.
[64]MIYA R K,FIRESTONE m K.Enhanced phenanthrene biodegradation in soils by slender oat root exudates and root debris[J].Journal of Environmental Quality,2001,30:1911-1918.
[65]YOSHITOMI K J,SHANNJ R.Corn(Zea maysL.) root exudates and their impact on ~(14)C-pyrene mineralization[J].Soil Biology & Biochemistry,2001,33:1769-1776.
[66]RENTZ J A,ALVAREZ P J J,SCHNOOR J L.Benzo[a]pyrene co-metabolism in the presence of plant root extracts and exudates:implications for phytoremediation[J].Environmental Pollution,2005,136:477-484.
[67]林道辉,朱立中,高彦征.土壤有机污染植物修复的机理与影响因素[J].应用生态学报,2003,14(10):1799-1803.
[68]陈静,王学军,胡俊栋.腐殖酸对土壤持留多环芳烃的影响[J].农业环境科学学报,2005,24(5):849-853.
[69]WEISSENFELS W D,KLEWER H J,LANGHOFF J.Adsorption of polycyclic aromatic hydrocarbons(PAHs) by soil particles:influence on biodegradability and biotoxicity[J].Applied Biochemistry and Microbiology,1992,36:689-696.
[70]Xu J G,Johnson R L.Nitrogen dynamics in soils with different hydro-carbon contents planted to barley and field pea[J].Canadian Journal of Soil Science,1997,77:453-458.
[71]Lin Qianxin,Mendelssohn I A.The combined effects of phytoremediation and biostim ulation in enhancing habitat restoration and oil degradation of petroleum contaminated we tlands[J].Ecol Engineering,1998,10(3):263-274.
[72]宋玉芳,许华夏,任丽萍.两种植物条件下土壤中矿物油和多环芳烃(多环芳烃)的生物修复研究[J].应用生态学报,2001,12(1):108-112.
[73]Del' Arco J P,Franca F P.Influence of oil contamination levels on hydrocarbon biodegradation in sandy sedment[J].Environ Pollut,2001,112(3):515-519.
[74]Trindade P V O,Sobral L G,R izzo A C L,et al.Bioremediation of a weathered and a recently oil-contaminated soils from B razil:a comparison study[J].Chemosphere,2005,58(4):515-522.
[75]平立凤,骆永明.有机质对多环芳烃环境行为影响的研究进展[J].土壤,2005,37(4):362-369.
[76]LEYVAL C,BINET P.Effect of polyaromatic hydrocarbons in soil on arbuscular mycorrhizal plants[J].Journal of Environmental Quality,1998,27:402-407.
[77]JOHNSON D L,MAGUIRE K L,ANDERSON D R,et al.Enhanced dissipation of chrysene in planted soil:the impact of a rhizobial inoculum[J].Soil Biology & Biochemistry,2004,36(1):33-38.
[78]TORSVIK V,GOKSOYR J,DAAE F L.High diversity in DNA of soil bacteria[J].Appl.Environ. Microbiol.,1990,56:782-787.
[79]吴方正.土壤的多环芳烃污染及其生物治理技术进展[J].土壤学进展,1995(1):32-44.
[80]Jonathan D.Van Hamme,Ajay Singh,Owen P.Ward.Recent Advances in Petroleum Microbiology[J].Microbiology and Molecular Biology Reviews.2003,67(4):503-549
[81]刘晓春,阎光绪,郭绍辉等.微生物降解土壤中石油污染物的研究进展[J].污染防治技术,2007,20(6):51-54,97.
[82]孙东平,胡凌燕,周伶俐,等.高效石油降解微生物堆制法处理油污土壤[J].环境科学与技术,2007,30(6):86-89.
[83]KAPLEY A,PUROHIT H J,CHHATRE S,et al.Osmotolerance and hydrocarbon degradation by a genetically engineered microbial consortium[J].Bioresource Technology,1999,67:241-245.
[84]Ghazoli F M,et al.Biodegradation of hydrocarbon in soil by microbial consortium[J].International Biodeterioration&Biodegradation,2004,54:61-67.
[85]Chhatre S,et al.Bacterial consortia for crude oil spill remediation[J].Wat Sci.Tech.1996,34(lO):187-193.
[86]张蔚文.混合菌培养在生物降解中的意义[J].环境科学与技术,1993,16-20.
[87]Matsson M.Carboxin resistance in Paracoccus denitrificans conferred by amutation in the membrane-anchor domain of succinate:quinone reductase.Archives of Microbiology [J].1998,170(1):37-47.
[88]Rahman K S M,et al.Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients[J].Bioresource Technology,2003,90:159-168.
[89]Zhao Y W,Wang S M,Zhang J F.Microbial treatment of groundwater contaminated by petroleuman application study[J].J.Appl.Ecol.1998,9(2):209-212
[90]RADWANS,SORKHOHN,EI-NEMR I.Oil biodegradiation around roots[J].Nature(London),1995,376:302.
[91]张金丽,郑天凌.多环芳烃污染环境的控制与生物修复研究进展[J].福建环境,2002,19(2):26-29.
[92]蔡燕飞,廖宗文.土壤微生物生态学研究方法进展[J].土壤与环境,2002,11(2):167-171
[93]李宝明.石油污染土壤微生物修复的研究[D].北京:中国农业科学院.
[94]GRISHCHENKOV V G,TOWNSEND R T,MCDONALD T J,et al.Degradation of petroleum hydrocarbons by facultative anaerobic bacteria under aerobic and anaerobic conditions[J].Process Biochemistry,2000,35(9):889-896.
[95]丁克强,骆永明.生物修复石油污染土壤[J].土壤,2001,(4):179-184,196.
[96]张锡辉.高等环境化学与微生物学原理及应用[M],北京:化学工业出版社,2001.
[97]Chang HL,hlvarez-cohen L.Model for the cometabolic biogradation of chlorinated organic[J].Enviro.Sci.Technol.,1995,29:2356-2367.
[98]HUANG X D,EI-ALAWI Y,PENROSE D M,et al.A multi-process phytoremediation system for removal of polycyclic aromatic hydrocarbons from contaminated soils[J].Environmental Pollution,2004,130(3):465-476.
[99]MIYA R K,FIRESTONE M K.Phenanthrene-degrader community dynamics in rhizosphere soil from a common annual grass[J].Journal of Environmental Quality,2000,29:584-592.
[100]RUGH C L,SUSILAWATI E,KRAVCHENKO A N,et al.Biodegrader metabolic expansion during polyaromatic hydrocarbons rhizoremediation[J].Zeitschrift Fur Naturforschung C-A Journal of Biosciences,2005,60:331-339.
[101]MURATOVA A,HUBNER T,TISCHER S,et al.Plant-rhizosphere-microflora association during phytoremediation of PAH-contaminated soil[J].International Journal of Phytoremediation,2003,5(2):137-151.
[102]JONER E J,JONANSEN A,LOIBNER A P,et al.Rhizosphere effects on microbial community structure and dissipation and toxicity of polycyclic aromatic hydrocarbons(多环芳烃) in spiked soil[J].Environmental Science & Technology,2001,35(13):2773-2777.
[103]JOHNSON D L,ANDERSON D R,MCGRATH S P.Soil microbial response during the phytoremediation of a PAH contaminated soil[J].Soil Biology & Biochemistry,2005,37:2334-2336.
[104]金文标,宋莉辉,董晓利等.油污土壤微生物治理的影响因素[J].环境保护,1998(10):27-28.
[105]苏荣国,牟伯中,王修林等.微生物对石油烃的降解机理及影响因素[J].化工环保,2001,2l(4):205-208.
[106]袁红莉.降解石油微生物菌种的筛选及降解特性[J].中国环境科学,2003,23(2).157-161
[107]张春桂,张海荣,马学军,等.石油污染土壤长料堆式异地生物修复技术研究[J].应用生态学报,2001,12(2):279-282.
[108]周启星.复合污染生态毒理效应的定量关系分析[J].中国科学C辑.2003,33(6):566-573.
[109]Li G,Huang W,Lerner D N,et al.Enirchment of degrading micorbes and bioremediation of petrochemical contaminants in polluted soil.Water Research,2000,34(15):3845-3853.
[110]DIRK B,JOACHIM R,KARL S.Bioremediation of Diesel Fuel Contaminated Soil in a Rotating Bioreactor Part Ⅰ:Influence of Oxygen Saturation[J].Chem.Eng.Technol.,1998,21(2):168-172.
[111]Carriere P.P.E.,Mesania F.A.Enhanced biodegradation of creosote-contaminated soil[J].Waste Management.1995,15(8):579-583.
[112]金文标,宋莉晖,董晓利.盐度对油污土壤生物治理的影响[J].钻采工艺,1998,2l(4):71-72.
[113]RITTER W F.SCARBOROUGH R W.A review of bioremediation of contaminated soils and ground water[J].Environ.Sci.Health A,30(2):333-357.
[114]PELLETIERA E,DELILLE D,DELILLE B.Crude oil bioiemediation in sub-Antarctic intertidal sediments:chemistry and toxicity of oiled residues[J].Marine Environmental Research,2004(57):311-327.
[115]Hamoda M F,Al-Atlar I M S.Effect of high sodium chloride concentration on activated sludge treatment[J].Water Sci Tech,1995(27):7-8.
[116]陈玉成.污染环境生物修复工程[M].北京化学工业出版社,2003:1-34.
[117]李兴伟.石油类有机污染物在土壤中迁移数值模拟[D].大庆:大庆石油学院,2005.
[118]Muratova A Y,Turkovskaya O V,H(u|¨)bner T,et al.Studies of the efficacy of alfalfa and reed in the phytore-mediation of hydrocarbon-polluted soil[J].Appl.Biochem.Microbiol.,2003,39(6):599-605.
[119]Merkl N,Schultze-kraft R,Infante C.Assessment of tropical grasses and legumes for phytoremediation of petroleumcontam in ated soils[J].Water,Air,and Soil Pollution,2005,165(1):195-209.
[120]Li X,Feng Y,Sawatsky N.Importance of soil water relations in assessing the endpoint of bioremediated soils:Ⅰ.Plant growth[J].Plant and Soil,1997,192:219-226.
[121]张松林,赵首彩.紫花苜蓿在紫油污染土壤中的萌发状况[J].生态环境,2004,13(4):569-571.
[122]李春荣,王文科,曹玉清.石油污染土壤对黄豆生长的影响[J].安全与环境学报,2007,7(4):28-30.
[123]Ogboghodo I A,Iruaga E K,Osemwota I O,et al.An assessment of the effects of crude oil pollution on soil properties,germination and growth of maize(Zea mays) using two crude types-forcados light and escravos light[J].Environmental Monitoring and Assessment,2004,96:143-152.
[124]孙铁珩,宋玉芳.土壤污染的生态毒理诊断[J].环境科学学报,2002,22(6):689-695.
[125]宋玉芳,许华夏等.土壤重金属对白菜种子发芽与根伸长抑制的生态毒性效应[J].环境科学,2002,23(1):103-107.
[126]陆秀君.植物法生物修复石油污染土壤研究[D].沈阳:沈阳农业大学,2003.
[127]卢丽丽.石油污染土壤的植物修复研究[D].西安:西安建筑科技大学,2008.
[128]明道绪.生物统计附试验设计[M].中国农业出版社,2002:75-206.
[129]谢重阁.环境中石油污染物的分析技术[M].北京:中国环境科学出版社,1987,82-83.
[130]Wei S-H,Zhou Q-X,Zhang K-Set al.Roles of rhizosphere in remediation of contaminated soils and its mechanisms[J].Chinese Journal of Applied Ecology,2003,14(1):143-147.
[131]李云辉,文荣联,莫测辉.土壤有机污染的植物修复研究进展[J].广东农业科学,2007,(12)96-98.
[132]范淑秀,李培军,何娜等.多环芳烃污染土壤的植物修复研究进展[J].农业环境科学学报2007,26(6):2007-2013.
[133]刘晓冰,邢宝山,周克琴,等.污染土壤植物修复技术及其机理研究[J].中国生态农业学报[J],2005.13(1):134-138.
[134]Gunther T,Dornberger U,Fritsche W.Effects of ryegrass on biodegradation ofhydrocarbons in soil[M].Chemosphere,1996,33(2):203-215.
[135]JUAN C C,MARTA C,ANGELICA M A.Biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and pure cultures of imperfect and lignolitic fungi[J].Environmental Pollution,1996,94:355-362.
[136]刘鹏,李大平,王晓梅,等.石油污染土壤的生物修复技术研究[J].化工环保,2006,26(2):91-94.
[137]张海荣,李培军,孙铁珩,等.四种石油污染土壤生物修复技术研究[J].农业环境保护,2001,20(2):78-80.
[138]何翊,吴海,魏薇.石油污染土壤菌剂修复技术研究[J]土壤,2005,37(3):338-340.
[139]丁明宇,黄健,李永祺.海洋微生物降解石油的研究[J].环境科学学报,2001,21(1):84-88.
[140]刘国良,苏幼明,顾书敏,等.石油污染土壤生物修复研究新进展[J].化学与生物工程,2008,25(8):84-88.
[141]周群英,高廷耀.环境工程微生物学[M].北京:高等教育出版社,115-146.
[142]李文利,王忠彦,胡永松.土壤和地下水石油污染的生物治理[J].重庆环境科学,1999,21(4):35-37.
[143]武秀琦.西北黄土地区石油污染土壤生物修复影响因素研究[D].西安:西安建筑科技大学,2007.
[144]GEERDLNK M J.Model for microbial degradation of nonpolar organic contaminants in a soil slurry reactor[J].Environ Sci technol,1996,30:779-786.
[145]王红旗,陈延君,孙宁宁.土壤石油污染物微生物降解机理与修复技术研究[J].地学前缘,2006,13(1):134-139.
[146]蔺昕.石油烃污染土壤植物-微生物联合修复及机理研究[D].沈阳市:中国科学院沈阳生态 研究所,2005.
[147]Macek T,MackovaM,Kas J.Exploitation of plants for the removal of organics in environmental remediation[M].Biotechnology Advances,2000,18:23-34.
[148]章家恩.生态学常用实验研究方法与技术[M].北京:化学工业出版社,2007:61-62.
[149]李云雁,胡传荣.试验设计与数据处理[M].北京:化学工业出版社,2005.83-92.
[150]田胜元,肖日嵘编著.试验设计与数据处理[M].北京:建筑工业出版社,1993.11.
[151]袁志发,周静芋主编,试验设计与分析[M].北京:高等教育出版社,2000.
[152]韩慧龙,陈镇,杨健民,等.真菌-细菌协同修复石油污染土壤的场地试验[J].环境科学,2008,29(2):454-461.
[153]张新中,章玉平.植物生理学[M].北京:化学工业出版社,2007:227-229.
[154]乔玉辉,李花粉,马祥爱.污染生态学[M].北京化学工业出版社,2008:72-78,170-175.
[155]侯彦林,王曙光,郭伟.尿素施肥量对土壤微生物和酶活性的影响[J].土壤通报,2004,35(3):303-306.
[156]张丽荣,沈瑞清,康萍芝,等.尿素施用量对不同土壤中微生物的影响[J].宁夏农林科技,2004,(6):31-32.
[157]李晓磊,李井会,宋述.秸秆有机肥改善设施黄瓜连作土壤微生物区系[J].长春大学学报,2006,16(6):119-122.
[158]张信娣,曹慧,徐冬青,等.光合细菌和有机肥对土壤主要微生物类群和土壤酶活性的影响[J].土壤,2008,40(3):443-447.
[159]ANDERSON T A,GUTHRIE E A,WALTON B T.Bioremediation in the rhizosphere[J].Environmental Science & Technology,1993,27(13):2630-2636.
[160]严小龙,廖红,年海.根际生物学原理与应用[M].北京科学出版社,2007:115-157.
[161]周东美,郝秀珍,薛艳,等.污染土壤的修复技术研究进展[J].生态环境,2004,13(2):234-242.
[162]陆秀君.植物法生物修复石油污染土壤研究[D].沈阳市:沈阳农业大学,2003.