大蒜油对亚慢性铅中毒家兔排铅作用的研究
摘要
本文选用家兔20只，雌雄对半，随机分为5组，每组4只。Ⅰ组为阴性对照组，Ⅱ组为阳性对照组，Ⅲ、Ⅳ、Ⅴ组为喂饲大蒜油组。阳性对照组和喂饲大蒜油组以1.57mg/kg的剂量隔日腹注Pb(Ac)，溶液20天，同时喂饲大蒜油组每天分别喂饲70mg/kg，140mg/kg，420mg/kg的大蒜油，连续观察40天。从实验开始每5天收集各组家兔的尿、粪，实验结束后用原子吸收法测定微波消解处理过的尿、粪、脑、肝、肾、骨和血液中的铅及锌、铁、铜含量。阳性对照组的尿、粪及各组织器官中铅含量和血铅浓度明显高于阴性对照组。喂饲大蒜油各组的尿、粪排铅量明显高于阳性对照组，粪排铅量远大于尿排部铅量，且排铅量随大蒜油剂量的增大而增大；这三组的血铅浓度则在正常水平附近甚至更低，其各组织器官中的铅含量明显低于阳性对照组，而且各组织器官中的铅含量随大蒜油剂量的增大而减少。阳性对照组的尿锌、铁、铜含量明显高于阴性对照组和喂饲大蒜油各组，而喂饲大蒜油组的尿中微量元素锌、铁、铜含量与阴性对照组无显著性差异，且随大蒜油剂量的增大而减少。与阴性对照组相比，染毒使阳性对照组的血锌、铁浓度降低，血铜浓度升高；喂饲大蒜油的Ⅳ-Ⅴ组血锌、铁、铜浓度则与阴性对照组无显著性差异，血中锌、铁浓度明显高于阳性对照组，血铜浓度比阳性对照组低。阳性对照组器官中的锌、铁、铜含量明显低于阴性对照组；除肝铁外，喂饲大蒜油组的脑、肝、肾器官中锌、铁、铜含量均高于阴性对照组，其含量随大蒜油剂量的增大而增大。Ⅱ-Ⅴ组
四川大学顷十学位论文
——
各组织器官中的锌、铁、铜含量与对应各组的器官铅含量呈负相关：血中锌、
铁浓度与血铅浓度呈负相关，血铜浓度与血铅浓度呈正相关。本实验首次表明
大蒜油对草食类家兔有降低其体内铅蓄积量，促进铅排泄的驱铅解毒作用，特
别是对脑组织的保护作用最大而且在大蒜油解铅毒的同时没有传统驱铅剂那种
络合副作用，不会造成微量元素的损失。同时发现微量元素铜、锌、铁与铅之
间存在桔抗作用，为研究大蒜及其产品驱铅解毒的作用机理提供了有用信息和
新的动物模型。
20 rabbits were divided into I - V five groups (4 rabbits per group) randomly, the I group was fed normally as negative control group; the II group as positive group. The II-V four groups were injected lead acetate solution into their abdomen at a dosage of 1.57mg/kg every 48 hours for 20 days. Simultaneously, except the II group, the IIL iV N V three groups were fed every day with garlic oil at a dosage of 70mg/kg, 140mg/kg and 420mg/kg respectively, continuously observing for 40 days. Urine and feces of each group rabbits were collected ever}' 4 days. The content or concentration lead. Zinc, Ferrum and Copper in urine, feces, organs, tissue and blood of five groups rabbits was determined by AAS after the 40 days. The result showed that the lead content in urine, feces. brain, liver, kidney, bone and lead concentration in blood of the II group rabbits were significantly higher than that of the I group; the lead content in mine and feces of the III- Y groups was significantly higher than that of the II group, lead content in feces was higher than that in urine, and the more dosage of garlic oil . the more lead excretion of III-V groups: lead content in organs and tissue of the III- V group rabbits was significantly lower than that of the II group; lead concentration in blood of the III- V groups retained the level of the I group, and
3
the more dosage of garlic oil , the lower that of III-V groups. The content of Zinc, Ferrum and Copper in urine of the II group was significantly higher than that of the I and III- V group rabbits; the content of Zinc, Ferrum and Copper in urine of the III- V groups rabbits had no significant difference compared with that of the I group, and the more dosage garlic oil, the lower that of III-V groups. Compared with the I group, the concentration of Zinc, Ferrum in blood of the II group rabbits failed, and the Copper concentration in blood of the II group raised. The concentration of Zinc, Ferrum, Copper in blood of the IV-V groups had no significant difference compared with that of the I group; the concentration of Zinc and Ferrum in blood of the III-V groups was higher than that of the II group, the concentration of Copper in blood was lower than that of the II group. The content of Zinc, Ferrum, Copper in organs of the II group was significantly lower than that of the I group; except the content of Ferrum in liver ,the content of Zinc, Ferrum, Copper in organs of the III- V groups was higher than that of the I group, and the more dosage garlic oil, the more that of III-V groups. The Zinc, Ferrum and Copper contents in organs and Zinc, Ferrum concentration in blood of II - V groups was negatively related with lead contents or concentration of the corresponding groups, and Copper concentration in blood of II - V groups was positively related with lead concentration in blood of the corresponding groups. The experiment indicated firstly that the garlic oil could advance the excretion of the lead and reduce the lead accumulation level in tissue and organs of rabbits, especially protecting brain from poisoning. Garlic oil had no significant side effect compared with the traditional antidote of lead poisoning, and not lack of trace elements as Zinc, Ferrum and Copper, etc. Simultaneously, it was found that the content of Zinc, Ferrum and copper had oppositional effect on lead content. The study gave some valuable information for study of active substances and mechanism of detoxification on lead poisoning of garlic or its products and new animal model.
四川大学顷十学位论文
——
各组织器官中的锌、铁、铜含量与对应各组的器官铅含量呈负相关：血中锌、
铁浓度与血铅浓度呈负相关，血铜浓度与血铅浓度呈正相关。本实验首次表明
大蒜油对草食类家兔有降低其体内铅蓄积量，促进铅排泄的驱铅解毒作用，特
别是对脑组织的保护作用最大而且在大蒜油解铅毒的同时没有传统驱铅剂那种
络合副作用，不会造成微量元素的损失。同时发现微量元素铜、锌、铁与铅之
间存在桔抗作用，为研究大蒜及其产品驱铅解毒的作用机理提供了有用信息和
新的动物模型。
20 rabbits were divided into I - V five groups (4 rabbits per group) randomly, the I group was fed normally as negative control group; the II group as positive group. The II-V four groups were injected lead acetate solution into their abdomen at a dosage of 1.57mg/kg every 48 hours for 20 days. Simultaneously, except the II group, the IIL iV N V three groups were fed every day with garlic oil at a dosage of 70mg/kg, 140mg/kg and 420mg/kg respectively, continuously observing for 40 days. Urine and feces of each group rabbits were collected ever}' 4 days. The content or concentration lead. Zinc, Ferrum and Copper in urine, feces, organs, tissue and blood of five groups rabbits was determined by AAS after the 40 days. The result showed that the lead content in urine, feces. brain, liver, kidney, bone and lead concentration in blood of the II group rabbits were significantly higher than that of the I group; the lead content in mine and feces of the III- Y groups was significantly higher than that of the II group, lead content in feces was higher than that in urine, and the more dosage of garlic oil . the more lead excretion of III-V groups: lead content in organs and tissue of the III- V group rabbits was significantly lower than that of the II group; lead concentration in blood of the III- V groups retained the level of the I group, and
3
the more dosage of garlic oil , the lower that of III-V groups. The content of Zinc, Ferrum and Copper in urine of the II group was significantly higher than that of the I and III- V group rabbits; the content of Zinc, Ferrum and Copper in urine of the III- V groups rabbits had no significant difference compared with that of the I group, and the more dosage garlic oil, the lower that of III-V groups. Compared with the I group, the concentration of Zinc, Ferrum in blood of the II group rabbits failed, and the Copper concentration in blood of the II group raised. The concentration of Zinc, Ferrum, Copper in blood of the IV-V groups had no significant difference compared with that of the I group; the concentration of Zinc and Ferrum in blood of the III-V groups was higher than that of the II group, the concentration of Copper in blood was lower than that of the II group. The content of Zinc, Ferrum, Copper in organs of the II group was significantly lower than that of the I group; except the content of Ferrum in liver ,the content of Zinc, Ferrum, Copper in organs of the III- V groups was higher than that of the I group, and the more dosage garlic oil, the more that of III-V groups. The Zinc, Ferrum and Copper contents in organs and Zinc, Ferrum concentration in blood of II - V groups was negatively related with lead contents or concentration of the corresponding groups, and Copper concentration in blood of II - V groups was positively related with lead concentration in blood of the corresponding groups. The experiment indicated firstly that the garlic oil could advance the excretion of the lead and reduce the lead accumulation level in tissue and organs of rabbits, especially protecting brain from poisoning. Garlic oil had no significant side effect compared with the traditional antidote of lead poisoning, and not lack of trace elements as Zinc, Ferrum and Copper, etc. Simultaneously, it was found that the content of Zinc, Ferrum and copper had oppositional effect on lead content. The study gave some valuable information for study of active substances and mechanism of detoxification on lead poisoning of garlic or its products and new animal model.
引文
1．廖百基．环境中微量重金属元素的污染危害与迁移转化[M]．北京科学出版社．1989．69-97
2．熊海金等．国外防治儿童铅中毒的主要经验[M]．1999，16(6)：75-377
3. Chronopoulos,J.,et al,Variation in plant and soil lead and cadmium content in urban parks in Athens, Greece. Sci. Total Environ. 1997.196(1):91-98
4．陆龙根．影响儿童血铅水平的因素探讨．世界元素医学．2000，7(4)：19-21
5．郑星全等．北京市学龄儿童血铅水平及其与成人血铅水平的关系[J]．卫生研究，1993,22(增刊)：29-33
6. Goyer RA. Results of lead research: Prenatal exposure and neurological consequences. Environ Health Perspect, 1996,104(10): 1050-1054
7．李宏，符绍莲．铅的脑发育毒性机理，国外医学卫生分册。1997．24(3)：133-135
8. Klein M,et al. Lead poisoning in pregnancy. Biochem Pharmacol, 1991,41(4):479-484
9．薛红．锌、铁、铜、铅和小儿智力行为，广东微量元素科学．2000．7(6)：8-10
10. Lucchi L, Memo M. Chronic Lead Treatment Induce In Rat A Specific And Differential Effect On Dopamine Receptors In Different Brain Areas. Brain Research. 1981,213:397
11．许绍芬等，神经生物学[M]．上海医科大学出版社，1990．115-116
12．蔡宏道．环境污染与卫生监测．[M]．北京：人民卫生出版社．1979．55-62
13. Lamola, A.A.,et al, Zinc Protoporphyrin in the Erythrocytes of Patients with lead Intoxication and Iron Deficiency Anemia. Science. 1974,186:936
14．朱莲珍 主译校．人和动物的微量元素营养(Trace Elements In Human And Animal Nutrition)青岛出版社第一版．1994，8．630
15. Gould C.E.,et al, Trace Elements In human Biology And A Preliminary On A Possible Relationship To Multiple Sclerosis. Science Of The Total Environment. 1980,15(3):261
16．王粉荣，孟如意等，铅中毒与消化系统的变化及临床分析．世界元素医学．2000，7(1)：33
17．徐成伟，孙绍秋．铅中毒肾性病国内研究进展．见秦俊法等．铅污染的危害与防治研究．香港新闻出版社．1997，第一版．17
18．四种金属化合物对小鼠生殖细胞DNA损伤的研究．卫生毒理学杂志．2000，14(4)：227-228
19．纪云晶．实用毒理学手册．[M]．中国环境科学出版社．1991．389-407
20．王夔．生命科学中的微量元素．[M]．北京：中国计量出版社．1991
21．窄尾益英等．微量元素与疾病．周性海等译．北京：人民军医出版社．1987
22. Sugawara. E et al., Lipid peroxidation and concentration of gentathione in erythsoeytes from worker exposed to lead.[J].Br J Ind. Med, 1991,48(4):239
23．郭维新等．高铅负荷致氧化损伤及其保护作用观察．[J]．中国公共卫生学报．1994，13(6)：352-354
24．秦俊法．微量元素与骨质疏松．广东微量元素科学．1998，5(8)：5-14
25．陈钟英，刘无培等．临床药物手册．上海科技出版社．第三版．1995．621-631
26. Catilena LR and Klaassen CD. The effects of chelating agents on the excretion of endogenous metals. Toxicology and Pharmacology. 1982,63:344
27. Tandon SK, Jain VK. Effect of metal chelators on excretion and tissue level of essential trace elements. Environmental Research. 1984,35:237
28. Tandon SK, Flora SJS and Singh S. Chelation in metal intoxication XIV: comparative effects of thiol and amino chelators on lead poisoned rats with normal or damaged kidneys. Toxicol Appl Pharmcol.1985,79:204
29. Doolen P.D., et al., An evaluation of the nephrotoxicity of EDTA and DTPA in the rat.[J]. Toxicol.Appl.Pharm., 1967,10(3):481-500
30．孙世荃等，重金属促排螯合剂对肾脏毒性作用的实验研究．[J]．中华病理学杂志．1987，16(3)：202-205
31．梁冰，李晓兵，张伟等．铅的污染危害及天然产物防治铅中毒．四川环境．2000，19(2)：17-21
32．陈椿寿．重要的无机化学反应．第二版．上海：上海科技出版社．1982．2-3
33．蒋云生．碘化钾治疗铅性肾病的临床及实验研究．湖南医科大学学报．1994，4．99-101
34. Ghazaly KS. Influences of thiamin on lead intoxicatice lead deposition in tissues and lead hematological response of tiapia. Zillii. Comp. Biochem. Physiol, 1991, 100(5):417-421
35. Mait SK. L Swarup D, Chandra SV. Therapeutic potential of thiaminhy drochlorede chronic lead intoxication in goate. Res. Vet. Sci.,1990.48(3):377-388
36．段茂德，赵天保等．硫胺素驱铅30例疗效观察．中华劳动卫生职业病杂志．1998，4(5)：290
37．吴怡，杨华平．金属硫蛋白对大鼠体内铅促排作用的研究．环境与健康杂志．1998，15(6)：251-255
38．许长安等．肠吸附剂治疗铅中毒．[J]．铁道劳动安全卫生与环保．1994，21(4)：309
39．赵英环，刘建红．中药驱铅例临床疗效观察．职业医学．1992，19(1)：21
40．白雪涛，张洪桥等．珍珠提取物对铅暴露低龄大鼠及胎鼠组织铅负荷的影响中国公共卫生．2000，16(7)：603-604
41．魏肖莹．葵花盘低酯果胶驱铅临床研究．见：秦俊法等主编．铅污染的危与防治研究．香港新闻出版社，1997．第一版．
42．黄雪梅，张宏伟等．参芪散Ⅱ号治疗儿童铅吸收的临床与实验研究，中医杂志．1997，38(9)：551-552
43．王世俊．金属中毒．第二版．北京：人民卫生出版社．1980．
44．洛阳市职业病防治研究所等．中南区职业中毒防治经验交流会资料选编．[R]．1975．38
45．尹华，包其富等．大蒜驱铅的实验研究．河南中医药学刊，1999．14(4)：22-23
46．陈代雄、姜东、张永华等．刺梨多糖对动物免疫功能的影响．遵义医学院学报 1991，14(1)：1
47．石玉成、梁光义等．刺梨主要成份的淋巴细胞增殖作用．贵阳中医学院学报 1991，(2)：57
48．郭镇楚．甲壳素研究进展．日用化学工业．[J]．1997，2．29-32
49．张幼辰．膳食因素的排铅作用．见秦俊法等．铅污染的危害与防治研究．香港新闻出版社．1997．第一版．390
50．谭茵，王瑞淑．魔芋精粉对五种二价金属离子的体外结合研究．现代预防医学．1995，22(4)：195
51．张立实，王瑞敏．魔芋精分对大鼠消化道吸收和慢性铅中毒的影响．现代预防医学．1998，25(1)：79-81
52．李艳华等．大蒜对甲基汞中毒小鼠体内巯基含量的影响．[J]．环境与健康杂志．1993，10(1)：29
53.Lee Young O.K., et al., A study on the effect of garlic, D-penicil lamine and N-acetyl-DL-penicillamine against cadmium poisoning in rat[J].Koryo-Taehokkyo Uikwa. Taehok, Nonmunjip, 1986,23(3):43-53
54.Kim Byung Doo, et al., A study on the effect of garlic, 2,3-dimercaptosuccinic acid and N acetyl-DL-penicillamine on excretion of cadmium in rat[J]. Koryo-Taehokkyo Uikwa, Taehok, Nommunjip, 1987, 24(1):223-226
55.Song Tae Bok, et al., A study on the effect of garlic and 2,3-dimercaptosuccinic acid on the cadmium poisoning of pregnant rat[J]. Koryo-Taehokkyo Uikwa. Taehok.Nonmunjip. 1987,24(1):237-248
56.Cha Chul Whan, A study on the effect of garlic on heavy metal poisoning of rat[J]. J.Ko rean Med. Sci., 1987,2(4):213-224
57.Sheo Hwa-Jung, et al.,Effect of garlic juice on toxicity of lead in rat compared with N-a cetylpenicillamine action[J]. Han' guk Yongyang Siklyong Hakhoechi. 1996,25(1):27-33
58．尹华．大蒜排铅的机理研究．浙江中医学院学报．1999．23(1)：61
59．魏泓．医学实验动物学．四川科学技术出版社．1998，3．263
60. Cynthia P.Bosnak and Karen W. Barnes. A streamlined flame atomic absorption method for animal feed analysis. Atomic Spectroscopy. 1998,19(2):40-42
61. Ching-Jyi Hong. Shinne-Ren Lin, Determination of urinary trace elements (As.Hg,Zn,Pb.S e) in patients with black foot disease.Talanta. 1997,145:75-83.
62. Gwen M.Schelkoph and David B.Milne et al;Wet microwavedigestion of diet and fecal sa mples for inductively coupled plasma analysis. Anal. Chem. 1988,60:2060-2062
63．史菊妹，邹孝芳．石墨炉原子吸收光谱法分析全血中铅．理化检验(化学分册)．1986,22(4)：246-247
64．王簃兰主编．劳动卫生学．第三版．人民卫生出版社．1992．40
65．张印德，杨树媛等主编．铅、苯、汞中毒的防治．天津科学出版社．1985，9．第一版．
66．张德荣译 金属毒理学手册 成都：四川科学技术出版社，1985．339-345
67．李寿祺，王国钦等主编．卫生毒理学基本原理和方法．四川科学技术出版社．1981．23．
68. Goyer R A, Lead toxicity: Current concerns.Environ Health Perspectives, 1993,100:177-187
69. Pounds J G, Long G J, Rosen J F. Cellular and molecular toxicity of lead in bone. Envir on Health Perspectives, 1991,91:17-32
70．Goyer R A著，崔彦红 节译．见：秦俊法等．铅污染的毒害与防治研究．香港新闻出版社．1997．第一版．39．
71. Tandon S R et al. Influence of methionine and zinc supplementation during chelation of lead in rats. J Trace Elem. Electrolytes Health Dis, 1994,8:75
72. Satija N K et al. Preventive action of zinc against lead toxicity. Indian J Physiol Pharma col. 1995,39:377
73．沈晓明，郭迪等．铁剂和维生素C对铅所致幼鼠学习能力损害的预防性保护作用的实 验研究．见：秦俊法等主编．铅污染的危害及防治研究．香港新闻出版社．1997．
74．白雪涛，刘建荣等．钙、锌、铁、硒及B族维生素联合应用对大鼠小肠铅吸收的影响．广东微量元素科学．1998，5(4)：62-63
75．程怀民，张宏永等．长期低浓度铅暴露对人体微量元素的影响．广东微量元素科学．2000，7(1)：30-32
76．李凭建，朱士雅，Goyer，Robert A．实验性铅染毒对大鼠体内锌、钙的影响．广东微量元素科学．1998，5(4)：34-37
77．谢南英，谢琢玺等．铅在人体的允许量及其与锌的相关性．广东微量元素科学．1998，5(4)：64-65
78．汪青红，李云珍等．接铅人群去血铅、锌、铜及有关因素的调查分析．见：秦俊法等主编．铅污染的危害与防治研究．香港新闻出版社，1997．329-330
79．李国华，郭维新等．校园大气铅污染对学生血铅、铜、锌和SOD的影响．见：秦俊法等主编．铅污染的危害与防治研究．香港新闻出版社，1997．331-333．
2．熊海金等．国外防治儿童铅中毒的主要经验[M]．1999，16(6)：75-377
3. Chronopoulos,J.,et al,Variation in plant and soil lead and cadmium content in urban parks in Athens, Greece. Sci. Total Environ. 1997.196(1):91-98
4．陆龙根．影响儿童血铅水平的因素探讨．世界元素医学．2000，7(4)：19-21
5．郑星全等．北京市学龄儿童血铅水平及其与成人血铅水平的关系[J]．卫生研究，1993,22(增刊)：29-33
6. Goyer RA. Results of lead research: Prenatal exposure and neurological consequences. Environ Health Perspect, 1996,104(10): 1050-1054
7．李宏，符绍莲．铅的脑发育毒性机理，国外医学卫生分册。1997．24(3)：133-135
8. Klein M,et al. Lead poisoning in pregnancy. Biochem Pharmacol, 1991,41(4):479-484
9．薛红．锌、铁、铜、铅和小儿智力行为，广东微量元素科学．2000．7(6)：8-10
10. Lucchi L, Memo M. Chronic Lead Treatment Induce In Rat A Specific And Differential Effect On Dopamine Receptors In Different Brain Areas. Brain Research. 1981,213:397
11．许绍芬等，神经生物学[M]．上海医科大学出版社，1990．115-116
12．蔡宏道．环境污染与卫生监测．[M]．北京：人民卫生出版社．1979．55-62
13. Lamola, A.A.,et al, Zinc Protoporphyrin in the Erythrocytes of Patients with lead Intoxication and Iron Deficiency Anemia. Science. 1974,186:936
14．朱莲珍 主译校．人和动物的微量元素营养(Trace Elements In Human And Animal Nutrition)青岛出版社第一版．1994，8．630
15. Gould C.E.,et al, Trace Elements In human Biology And A Preliminary On A Possible Relationship To Multiple Sclerosis. Science Of The Total Environment. 1980,15(3):261
16．王粉荣，孟如意等，铅中毒与消化系统的变化及临床分析．世界元素医学．2000，7(1)：33
17．徐成伟，孙绍秋．铅中毒肾性病国内研究进展．见秦俊法等．铅污染的危害与防治研究．香港新闻出版社．1997，第一版．17
18．四种金属化合物对小鼠生殖细胞DNA损伤的研究．卫生毒理学杂志．2000，14(4)：227-228
19．纪云晶．实用毒理学手册．[M]．中国环境科学出版社．1991．389-407
20．王夔．生命科学中的微量元素．[M]．北京：中国计量出版社．1991
21．窄尾益英等．微量元素与疾病．周性海等译．北京：人民军医出版社．1987
22. Sugawara. E et al., Lipid peroxidation and concentration of gentathione in erythsoeytes from worker exposed to lead.[J].Br J Ind. Med, 1991,48(4):239
23．郭维新等．高铅负荷致氧化损伤及其保护作用观察．[J]．中国公共卫生学报．1994，13(6)：352-354
24．秦俊法．微量元素与骨质疏松．广东微量元素科学．1998，5(8)：5-14
25．陈钟英，刘无培等．临床药物手册．上海科技出版社．第三版．1995．621-631
26. Catilena LR and Klaassen CD. The effects of chelating agents on the excretion of endogenous metals. Toxicology and Pharmacology. 1982,63:344
27. Tandon SK, Jain VK. Effect of metal chelators on excretion and tissue level of essential trace elements. Environmental Research. 1984,35:237
28. Tandon SK, Flora SJS and Singh S. Chelation in metal intoxication XIV: comparative effects of thiol and amino chelators on lead poisoned rats with normal or damaged kidneys. Toxicol Appl Pharmcol.1985,79:204
29. Doolen P.D., et al., An evaluation of the nephrotoxicity of EDTA and DTPA in the rat.[J]. Toxicol.Appl.Pharm., 1967,10(3):481-500
30．孙世荃等，重金属促排螯合剂对肾脏毒性作用的实验研究．[J]．中华病理学杂志．1987，16(3)：202-205
31．梁冰，李晓兵，张伟等．铅的污染危害及天然产物防治铅中毒．四川环境．2000，19(2)：17-21
32．陈椿寿．重要的无机化学反应．第二版．上海：上海科技出版社．1982．2-3
33．蒋云生．碘化钾治疗铅性肾病的临床及实验研究．湖南医科大学学报．1994，4．99-101
34. Ghazaly KS. Influences of thiamin on lead intoxicatice lead deposition in tissues and lead hematological response of tiapia. Zillii. Comp. Biochem. Physiol, 1991, 100(5):417-421
35. Mait SK. L Swarup D, Chandra SV. Therapeutic potential of thiaminhy drochlorede chronic lead intoxication in goate. Res. Vet. Sci.,1990.48(3):377-388
36．段茂德，赵天保等．硫胺素驱铅30例疗效观察．中华劳动卫生职业病杂志．1998，4(5)：290
37．吴怡，杨华平．金属硫蛋白对大鼠体内铅促排作用的研究．环境与健康杂志．1998，15(6)：251-255
38．许长安等．肠吸附剂治疗铅中毒．[J]．铁道劳动安全卫生与环保．1994，21(4)：309
39．赵英环，刘建红．中药驱铅例临床疗效观察．职业医学．1992，19(1)：21
40．白雪涛，张洪桥等．珍珠提取物对铅暴露低龄大鼠及胎鼠组织铅负荷的影响中国公共卫生．2000，16(7)：603-604
41．魏肖莹．葵花盘低酯果胶驱铅临床研究．见：秦俊法等主编．铅污染的危与防治研究．香港新闻出版社，1997．第一版．
42．黄雪梅，张宏伟等．参芪散Ⅱ号治疗儿童铅吸收的临床与实验研究，中医杂志．1997，38(9)：551-552
43．王世俊．金属中毒．第二版．北京：人民卫生出版社．1980．
44．洛阳市职业病防治研究所等．中南区职业中毒防治经验交流会资料选编．[R]．1975．38
45．尹华，包其富等．大蒜驱铅的实验研究．河南中医药学刊，1999．14(4)：22-23
46．陈代雄、姜东、张永华等．刺梨多糖对动物免疫功能的影响．遵义医学院学报 1991，14(1)：1
47．石玉成、梁光义等．刺梨主要成份的淋巴细胞增殖作用．贵阳中医学院学报 1991，(2)：57
48．郭镇楚．甲壳素研究进展．日用化学工业．[J]．1997，2．29-32
49．张幼辰．膳食因素的排铅作用．见秦俊法等．铅污染的危害与防治研究．香港新闻出版社．1997．第一版．390
50．谭茵，王瑞淑．魔芋精粉对五种二价金属离子的体外结合研究．现代预防医学．1995，22(4)：195
51．张立实，王瑞敏．魔芋精分对大鼠消化道吸收和慢性铅中毒的影响．现代预防医学．1998，25(1)：79-81
52．李艳华等．大蒜对甲基汞中毒小鼠体内巯基含量的影响．[J]．环境与健康杂志．1993，10(1)：29
53.Lee Young O.K., et al., A study on the effect of garlic, D-penicil lamine and N-acetyl-DL-penicillamine against cadmium poisoning in rat[J].Koryo-Taehokkyo Uikwa. Taehok, Nonmunjip, 1986,23(3):43-53
54.Kim Byung Doo, et al., A study on the effect of garlic, 2,3-dimercaptosuccinic acid and N acetyl-DL-penicillamine on excretion of cadmium in rat[J]. Koryo-Taehokkyo Uikwa, Taehok, Nommunjip, 1987, 24(1):223-226
55.Song Tae Bok, et al., A study on the effect of garlic and 2,3-dimercaptosuccinic acid on the cadmium poisoning of pregnant rat[J]. Koryo-Taehokkyo Uikwa. Taehok.Nonmunjip. 1987,24(1):237-248
56.Cha Chul Whan, A study on the effect of garlic on heavy metal poisoning of rat[J]. J.Ko rean Med. Sci., 1987,2(4):213-224
57.Sheo Hwa-Jung, et al.,Effect of garlic juice on toxicity of lead in rat compared with N-a cetylpenicillamine action[J]. Han' guk Yongyang Siklyong Hakhoechi. 1996,25(1):27-33
58．尹华．大蒜排铅的机理研究．浙江中医学院学报．1999．23(1)：61
59．魏泓．医学实验动物学．四川科学技术出版社．1998，3．263
60. Cynthia P.Bosnak and Karen W. Barnes. A streamlined flame atomic absorption method for animal feed analysis. Atomic Spectroscopy. 1998,19(2):40-42
61. Ching-Jyi Hong. Shinne-Ren Lin, Determination of urinary trace elements (As.Hg,Zn,Pb.S e) in patients with black foot disease.Talanta. 1997,145:75-83.
62. Gwen M.Schelkoph and David B.Milne et al;Wet microwavedigestion of diet and fecal sa mples for inductively coupled plasma analysis. Anal. Chem. 1988,60:2060-2062
63．史菊妹，邹孝芳．石墨炉原子吸收光谱法分析全血中铅．理化检验(化学分册)．1986,22(4)：246-247
64．王簃兰主编．劳动卫生学．第三版．人民卫生出版社．1992．40
65．张印德，杨树媛等主编．铅、苯、汞中毒的防治．天津科学出版社．1985，9．第一版．
66．张德荣译 金属毒理学手册 成都：四川科学技术出版社，1985．339-345
67．李寿祺，王国钦等主编．卫生毒理学基本原理和方法．四川科学技术出版社．1981．23．
68. Goyer R A, Lead toxicity: Current concerns.Environ Health Perspectives, 1993,100:177-187
69. Pounds J G, Long G J, Rosen J F. Cellular and molecular toxicity of lead in bone. Envir on Health Perspectives, 1991,91:17-32
70．Goyer R A著，崔彦红 节译．见：秦俊法等．铅污染的毒害与防治研究．香港新闻出版社．1997．第一版．39．
71. Tandon S R et al. Influence of methionine and zinc supplementation during chelation of lead in rats. J Trace Elem. Electrolytes Health Dis, 1994,8:75
72. Satija N K et al. Preventive action of zinc against lead toxicity. Indian J Physiol Pharma col. 1995,39:377
73．沈晓明，郭迪等．铁剂和维生素C对铅所致幼鼠学习能力损害的预防性保护作用的实 验研究．见：秦俊法等主编．铅污染的危害及防治研究．香港新闻出版社．1997．
74．白雪涛，刘建荣等．钙、锌、铁、硒及B族维生素联合应用对大鼠小肠铅吸收的影响．广东微量元素科学．1998，5(4)：62-63
75．程怀民，张宏永等．长期低浓度铅暴露对人体微量元素的影响．广东微量元素科学．2000，7(1)：30-32
76．李凭建，朱士雅，Goyer，Robert A．实验性铅染毒对大鼠体内锌、钙的影响．广东微量元素科学．1998，5(4)：34-37
77．谢南英，谢琢玺等．铅在人体的允许量及其与锌的相关性．广东微量元素科学．1998，5(4)：64-65
78．汪青红，李云珍等．接铅人群去血铅、锌、铜及有关因素的调查分析．见：秦俊法等主编．铅污染的危害与防治研究．香港新闻出版社，1997．329-330
79．李国华，郭维新等．校园大气铅污染对学生血铅、铜、锌和SOD的影响．见：秦俊法等主编．铅污染的危害与防治研究．香港新闻出版社，1997．331-333．