鹿花盘中药协同食用菌发酵物抗疲劳作用研究
|
摘要
据世界卫生组织的一项调查表明：全球约75%的人处于亚健康状态,而疲劳正是众多亚健康状态中最常见的一种表现形式。当机体长时间处于疲劳状态而得不到及时缓解,就会出现一系列生理生化变化,使机体的各种机能下降,严重会伤及脏腑。中医药以其安全有效,毒副作用小等特性在缓解疲劳的发生或及时的清除疲劳感方面发挥重要作用,基于此,本论文进行如下研究：
1.分别将鹿花盘制备成鹿花盘胶和鹿花盘霜,提取水溶性蛋白,并对该蛋白进行分离纯化,测得蛋白分子量主要为14.9KD,27.6KD,60KD。根据2005版《中药药典》中规定的用药剂量给药,并按卫生部的《保健食品功能学评价程序与检验方法》对鹿花盘的三种不同加工产物进行抗疲劳作用的功能学评价。结果表明：鹿花盘胶和鹿花盘霜组显著(P<0.05)延长了小鼠游泳时间,鹿花盘蛋白组极显著(P<0.01)地延长游泳时间；三组均能显著(P<0.05)降低血尿素氮含量并极显著(P<0.01)增加肝糖原含量；鹿花盘霜和鹿花盘蛋白组能显著(P<0.05)增强乳酸脱氢酶活力,另外鹿花盘胶和鹿花盘蛋白对血乳酸含量有显著(P<0.05)影响。
2.以小鼠负重游泳时间为评价指标,分别比较益气健脾、疏肝理气、温阳补肾、活血四类植物中药对小鼠耐力的影响。结果表明：益气健脾、疏肝理气、活血及温阳补肾类植物中药均能提高小鼠的耐力。
3.液体培养五种食用菌,选择胞内多糖含量高时收集菌丝体,通过小鼠耐力试验发现五种食用菌菌体均能提高小鼠的耐力。
4.筛选复方正交试验,以游泳耐力为评价指标进行初筛,筛选出的复方进行抗疲劳理化指标测定,同时对其部分免疫指标和抗氧化指标进行测定。最佳复方具有显著抗疲劳作用的同时,对免疫指数,吞噬细胞的吞噬率有一定影响,同时能提高体内超氧化物歧化酶(SOD)含量,降低运动后小鼠体内丙二醛(MDA)的含量。
5.在冰淇淋基本配方中加入抗疲劳浓缩液,制备具有抗疲劳功能的保健型冰淇淋,通过感官评价结果表明,加入抗疲劳浓缩液后,冰淇淋口感更清新爽口。
The World Health Organization (WHO) survey has shown that about 75% of people in the world are in the sub-health state, while fatigue is the most common form of sub-health state. When the body is exposed to fatigue and lacking of mitigation timely, there will be a series of physiological and biochemical changes that decrease the body's functions and hurt organs seriously. Chinese traditional medicine, characterized by safety, efficacy and less side effects, plays an important role in alleviating and removaing fatigue. Therefore, we performed experiments in this study as follows:
1. Sika antler dish was prepared into glue and frost. In a separate experiment group, the water-soluble proteins of the Sika antler dish were extracted, followed with isolation and purification of proteins. Measurements showed that the molecular weight were 14.9kDa,27.6kDa and 60kDa, respectively. The anti-fatigue function of Sika antler dish products derived from three processing methods was assessed according to Functional Assessment Procedure And Test Methods For Health Food (China's Ministry of Health) with the dosage instructed by the Traditional Chinese Medicine Pharmacopoeia (China 2005 Edition). The results showed that Antlers dish glue group, Antlers dish frost group and Antlers dish protein group reduced the content of blood urea nitrogen and increased the content of glycogen significantly; both protein and frost groups apparently enhanced the activity of lactate dehydrogenase.
2. According to the index of the loaded-swimming time of mice. We test out the effect of the endurance of the mouse in the group with comparing the four types of plants, tonifying kidney, strengthening spleen, nourishing liver and promoting blood circulation traditional Chinese medicine. The results show that:The Chinese medicine of strengthening spleen, nourishing liver Chinese medicine, promoting blood circulation traditional Chinese medicine and tonifying kidney Chinese medicine all can obvious effect on its endurance.
3. By nurturing liquid fermentation five types of fungi, we collect mycelia when they have the highest content of the polysaccharide in cellulars. By the endurance experiment of the mouse, we found that these five types of edible fungi all can improve the endurance of mice.
4. Preliminary screening was performed by the evaluation indexes of the swimming endurance of mouse. Anti-fatigue physiochemical index, some immune indexes and oxidation indexes for the best compound prescription was determined. The results showed that the best compound prescription can elevate the hepatic glycogen, reduce blood urea nitrogen, improve the activity of lactic dehydrogenase and influence the lactic acid content; can also increase the immune index of the immune organs, increase the devouring rate of the phagocyte, improve the SOD content in human's body and lower the content of malonaldehyde (MDA) in mice after exercise.
5. Anti-fatigue concentrating liquid was added to the basic formula of ice cream for making the special anti-fatigue ice cream. The sensory evaluation showed that the flavor of ice cream containing the anti-fatigue concentrated liquid taste better than basic ice cream.
1.分别将鹿花盘制备成鹿花盘胶和鹿花盘霜,提取水溶性蛋白,并对该蛋白进行分离纯化,测得蛋白分子量主要为14.9KD,27.6KD,60KD。根据2005版《中药药典》中规定的用药剂量给药,并按卫生部的《保健食品功能学评价程序与检验方法》对鹿花盘的三种不同加工产物进行抗疲劳作用的功能学评价。结果表明：鹿花盘胶和鹿花盘霜组显著(P<0.05)延长了小鼠游泳时间,鹿花盘蛋白组极显著(P<0.01)地延长游泳时间；三组均能显著(P<0.05)降低血尿素氮含量并极显著(P<0.01)增加肝糖原含量；鹿花盘霜和鹿花盘蛋白组能显著(P<0.05)增强乳酸脱氢酶活力,另外鹿花盘胶和鹿花盘蛋白对血乳酸含量有显著(P<0.05)影响。
2.以小鼠负重游泳时间为评价指标,分别比较益气健脾、疏肝理气、温阳补肾、活血四类植物中药对小鼠耐力的影响。结果表明：益气健脾、疏肝理气、活血及温阳补肾类植物中药均能提高小鼠的耐力。
3.液体培养五种食用菌,选择胞内多糖含量高时收集菌丝体,通过小鼠耐力试验发现五种食用菌菌体均能提高小鼠的耐力。
4.筛选复方正交试验,以游泳耐力为评价指标进行初筛,筛选出的复方进行抗疲劳理化指标测定,同时对其部分免疫指标和抗氧化指标进行测定。最佳复方具有显著抗疲劳作用的同时,对免疫指数,吞噬细胞的吞噬率有一定影响,同时能提高体内超氧化物歧化酶(SOD)含量,降低运动后小鼠体内丙二醛(MDA)的含量。
5.在冰淇淋基本配方中加入抗疲劳浓缩液,制备具有抗疲劳功能的保健型冰淇淋,通过感官评价结果表明,加入抗疲劳浓缩液后,冰淇淋口感更清新爽口。
The World Health Organization (WHO) survey has shown that about 75% of people in the world are in the sub-health state, while fatigue is the most common form of sub-health state. When the body is exposed to fatigue and lacking of mitigation timely, there will be a series of physiological and biochemical changes that decrease the body's functions and hurt organs seriously. Chinese traditional medicine, characterized by safety, efficacy and less side effects, plays an important role in alleviating and removaing fatigue. Therefore, we performed experiments in this study as follows:
1. Sika antler dish was prepared into glue and frost. In a separate experiment group, the water-soluble proteins of the Sika antler dish were extracted, followed with isolation and purification of proteins. Measurements showed that the molecular weight were 14.9kDa,27.6kDa and 60kDa, respectively. The anti-fatigue function of Sika antler dish products derived from three processing methods was assessed according to Functional Assessment Procedure And Test Methods For Health Food (China's Ministry of Health) with the dosage instructed by the Traditional Chinese Medicine Pharmacopoeia (China 2005 Edition). The results showed that Antlers dish glue group, Antlers dish frost group and Antlers dish protein group reduced the content of blood urea nitrogen and increased the content of glycogen significantly; both protein and frost groups apparently enhanced the activity of lactate dehydrogenase.
2. According to the index of the loaded-swimming time of mice. We test out the effect of the endurance of the mouse in the group with comparing the four types of plants, tonifying kidney, strengthening spleen, nourishing liver and promoting blood circulation traditional Chinese medicine. The results show that:The Chinese medicine of strengthening spleen, nourishing liver Chinese medicine, promoting blood circulation traditional Chinese medicine and tonifying kidney Chinese medicine all can obvious effect on its endurance.
3. By nurturing liquid fermentation five types of fungi, we collect mycelia when they have the highest content of the polysaccharide in cellulars. By the endurance experiment of the mouse, we found that these five types of edible fungi all can improve the endurance of mice.
4. Preliminary screening was performed by the evaluation indexes of the swimming endurance of mouse. Anti-fatigue physiochemical index, some immune indexes and oxidation indexes for the best compound prescription was determined. The results showed that the best compound prescription can elevate the hepatic glycogen, reduce blood urea nitrogen, improve the activity of lactic dehydrogenase and influence the lactic acid content; can also increase the immune index of the immune organs, increase the devouring rate of the phagocyte, improve the SOD content in human's body and lower the content of malonaldehyde (MDA) in mice after exercise.
5. Anti-fatigue concentrating liquid was added to the basic formula of ice cream for making the special anti-fatigue ice cream. The sensory evaluation showed that the flavor of ice cream containing the anti-fatigue concentrated liquid taste better than basic ice cream.
引文
[1]李八方.功能食品与保健食品[M].青岛：青岛海洋大学出版社,1997.
[2]魏源.运动性疲劳研究进展的综述[J].韩山师范学院学报,2000,(02)：83-87.
[3]Toshihiko K. Brain mechanisms of poly I:C induced fatigue[M] Watanabe Y, Evengard B, Natels on B H, e t al. Fatigue science for human health. Hicom, Japan:S pringer,2008:219-230.
[4]梁俊荣.昆虫、菌藻抗疲劳功能性食品添加剂研究[D].中国人民解放军军需大学,2003,6.
[5]冯炜权.运动疲劳及过度训练的生化诊断[J].北京体育大学学报,2000,23(4)：286-288.
[6]Urke LM.J.A. Hanley. Carbohydrate and exercise [J].Current Opinion in Clinical Nutrition and Metablic Care.1999,2:515-520.
[7]Burke, L. M. Nutrition for post-exercise recovery[J]. Austrilian Journal of Science and Medicine in Sport,1997,(29):3-10.
[8]陈巍.中药复方制剂抗疲劳功效评价及其机理研究[D].中国人民解放军军需大学,2003.
[9]张濒,高顺生.运动性疲劳的研究进展[J].北京体育师范学院学报,2000,12(1)：72-76.
[10]唐量,熊正英.芦荟抗疲劳作用的实验[J].体育学刊,2003,10(2)50-52.
[11]李涛.略谈运动性疲劳的成因[J].安徽体育科技,2000(4)：100-102.
[12]Thompson LV Fitts RH Muscle in the frog semitendinosus:role of intracellular PH, Am J Physiol,1992(262):1507-1509.
[13]Roussean E,PH modulate conducting and gating behavior of single cacium Release channels, Pfluegers Arch,1990,415:645-647.
[14]Edman KA. Changes in force and intracellular acidification in frog muscle fibers, JPhysiolLond1990,424:133-149.
[15]Holloszy JO Biochemical adaptions in muscle, J BiolChem,1967,242:2278.
[16]田振军,石磊.过度运动对大鼠血清CK,LDH,SOD,SDH活性及UMb含量影响的研究[J].中国运动医学杂志,2000,19(1)：49-50.
[17]吕莹.牛英鹏运动性疲劳的机制.河南大学学报(自然科学版),2000,30(3)：61-64.
[18]候香玉.运动对自由基代谢的影响[J].体育科学,1991(4)：19-20.
[19]Davies JJA.Free radicals and tissue damage produced by exercise[J]. ApplPhysiol,1982(107):1198-1205.
[20]Brady PS. Selenium,Vita min E and the response to swimming stress in rat[J], Nutr,1997,109:1103-1109.
[21]李峰杨维益,梁嵘等.从中医学看肝脏调节应激反应的作用[J].北京中医药大学学,1998,21(1)：20-22.
[22]王振玉.鹿花盘注射液研究[J].生化药物杂志,1987,(2)：10-11.
[23]张宝香,赵延平.鹿角盘的化学成分与开发利用[J].特种经济动植物,2005,(18)：7.
[24]江苏新医学院.中药大辞典[M].上海：上海科学技术出版社,1986：2230-2231.
[25]李泽鸿,武丽敏,姚玉霞等.梅花鹿鹿茸不同产品中氨基酸含量的比较[J].氨基酸和生物资源,2007,29(3)：16.
[26]苏凤艳,李慧萍,王艳梅等.鹿花盘蛋白质的提取与生物活性测定[J].动物科学与动物医学,2001,18(2)：18-20.
[27]王志兵,邱芳萍,李志民等.鹿角盘活性成分对小鼠吞噬功能及对大鼠乳腺增生激素水平的影响[J].食品科学,2007,31(11)：225-226.
[28]王斌,张声华,李晓莉等.人参总皂甙的耐缺氧效应机理研究[J].食品科学,2002,23(8)：270-272.
[29]VOCES J, ALVAREZ A I,VILAL,et al. Effects of administration of the standardized panax ginseng extract G115 on hepatic antioxidant function after exhaustive exercise [J].Comp Biochem Physiol C Pharmacol Toxicol Endocrinol,1999,123(2):175-184.
[30]Nishibes. Phenolic compounds from stembark of Acanthopanax semicosus and their pharmacologtal effect inchwnic suirmmng stesseel rats[J]. Chem Pharm Bull,1990,38(6):1763.
[31]王庭欣,赵文,刘峥颢.西洋参片对小鼠抗疲劳作用的实验研究[J].食品科学,2005,26(9)：474-475.
[32]罗琼,阎俊,张声华.枸杞多糖对小鼠抗疲劳作用的影响[J].湖北医科大学学报,1999,20(4)：265-269.
[33]李国莉.枸杞多糖对小鼠耐力及体内抗氧化酶活性的影响[J].辽宁中医杂志,2002,29(10)：634.
[34]陈亚东,田长有.高山红景天对小鼠耐缺氧、抗疲劳及耐低温作用的影响[J].中国中医药科技,2002,9(3)：158.
[35]尹旭辉,杨成君,姜在福等.红景天对寒冷适应过程中正常人体免疫功能的调节作用[J].解放军预防医学杂志,1996,14(6)：405.
[36]龚梦娟,刘新民,王立为.肉苁蓉对阳虚小鼠抗疲劳作用的研究[J].中医药导报,2007,13(11)：8-10.
[37]杨毅,贾力莉,武玉鹏等.人工冬虫夏草抗疲劳、降血脂作用实验研究[J].陕西中医,2008,24(10)：46-48.
[38]梁俊荣,张西臣,柳增善等.几种食药用真菌的抗疲劳作用研究[J].中国农业通报,2004,20(1)：135-137.
[39]梅学仁等.灵芝对血管系统药理作用的研究[J].中草药,1982,13(7).
[40]王满福,胡淑萍,李健康等.灵芝液对运动员抗疲劳效果的实验观察[J].中国运动医学杂志,1999,18(1)：78-79.
[41]杨娟,吴谋成,张声华等.香菇蛋白多糖抗疲劳作用研究[J].营养学报,2001,23(4)：350-353.
[42]涂宏海,王彩云,谢玲等.三康胶囊抗疲劳作用及其机理的实验研究[J].解放军药学学报,2006,26(3)：174-176.
[43]王琳,王天芳,张翠珍等.消疲怡神口服液抗应激的实验研究—对强制冷水游泳大鼠行为及神经内分泌的影响[J].北京中医药大学学报,1999,22(3)：22-24.
[44]来英,严卫星,楼密密.保健食品抗疲劳作用试验方法研究[J].中国食品卫生1997,9(4)：1-6,24.
[45]郑建仙.功能性食品(第三卷).北京：中国轻工业出版社,1999
[46]何来英,严卫星,楼密密等.保健食品抗疲劳作用试验方法研究.中国食品卫生杂志,1997,9(4)：1荀,24
[47]毛跟年,许牡丹主编.功能食品生理特性与检测技术[M].北京：化学土业出版社,2005：3-9,351-383,665-666.
[48]邱芳萍,马波,王志兵等.鹿花盘蛋白的分离纯化与活性研究[J].长春工业大学学报,2007,28(3)：144-147.
[49]柴晓杰,崔喜艳.生物化学实验技术[M].吉林：吉林大学出版社,2003,8：59-60.
[50]LaemmLi U K.Nature,1970(227):680.
[51]国家药典委员会.中华人民共和国药典(2005版,一部)[M].北京：化学工业出版社,2005：225-226.
[52]刘景圣,孟先军主编.功能性食品[M].北京：中国农业出版社,2005：252-253.
[53]Naka tani A Han DH, Hansen PA, etal.Effect endurance exercise training on muscle glyocogen super compensation in rats.J A p pl:Physiol,1999,82 (2):711.
[54]万劲.中医补肾药、补脾药对运动员机能状态及运动能力影响的综合观察[J].中国运动医学杂志1994,13(4)：202-206.
[55]潘珊珊,张炎,陆爱云.中医药抗运动性疲劳的研究综述[J].上海体育学院学报,1999,23(2)：52-55.
[56]马莉.红景天苷抗疲劳作用及其机制的实验研究[D].第二军事医学,2006,7.
[57]钱风雷.补肾中药对大鼠运动性低血睾酮的调整作用[J].中国运动医学杂志,1998(4)：230.
[58]傅晓雪.黄芪的香菇发酵及发酵液免疫效果的研究[D].江南大学,2009,5.
[59]张雪香,张桂英,王宝贵.西洋参人参皂甙抗疲劳作用的实验研究[J].中国公共卫生学报,1998,17(4)：238-239
[60]黄增艳,范书铎,库页红景天对鼠抗疲劳作用机理的实验研究[J].中国医科大学学报,1998,27(2)：123-124.
[61]刘声远.高山红景天根茎中超氧化歧化酶活性测定及其种类鉴[J].1993,24(3)：125.
[62]李翼,柴剑波,赵伟国.西洋参抗疲劳作用及对迟发型超敏感反应单核吞噬细胞功能影响的实验研究[J].中华中医药学报,2007,25(10)：2002-2003.
[63]李基洪,孙昌波,彭培勇.冰淇淋生产工艺与配方[M].北京：中国轻工业出版社,2001：356-365.
[2]魏源.运动性疲劳研究进展的综述[J].韩山师范学院学报,2000,(02)：83-87.
[3]Toshihiko K. Brain mechanisms of poly I:C induced fatigue[M] Watanabe Y, Evengard B, Natels on B H, e t al. Fatigue science for human health. Hicom, Japan:S pringer,2008:219-230.
[4]梁俊荣.昆虫、菌藻抗疲劳功能性食品添加剂研究[D].中国人民解放军军需大学,2003,6.
[5]冯炜权.运动疲劳及过度训练的生化诊断[J].北京体育大学学报,2000,23(4)：286-288.
[6]Urke LM.J.A. Hanley. Carbohydrate and exercise [J].Current Opinion in Clinical Nutrition and Metablic Care.1999,2:515-520.
[7]Burke, L. M. Nutrition for post-exercise recovery[J]. Austrilian Journal of Science and Medicine in Sport,1997,(29):3-10.
[8]陈巍.中药复方制剂抗疲劳功效评价及其机理研究[D].中国人民解放军军需大学,2003.
[9]张濒,高顺生.运动性疲劳的研究进展[J].北京体育师范学院学报,2000,12(1)：72-76.
[10]唐量,熊正英.芦荟抗疲劳作用的实验[J].体育学刊,2003,10(2)50-52.
[11]李涛.略谈运动性疲劳的成因[J].安徽体育科技,2000(4)：100-102.
[12]Thompson LV Fitts RH Muscle in the frog semitendinosus:role of intracellular PH, Am J Physiol,1992(262):1507-1509.
[13]Roussean E,PH modulate conducting and gating behavior of single cacium Release channels, Pfluegers Arch,1990,415:645-647.
[14]Edman KA. Changes in force and intracellular acidification in frog muscle fibers, JPhysiolLond1990,424:133-149.
[15]Holloszy JO Biochemical adaptions in muscle, J BiolChem,1967,242:2278.
[16]田振军,石磊.过度运动对大鼠血清CK,LDH,SOD,SDH活性及UMb含量影响的研究[J].中国运动医学杂志,2000,19(1)：49-50.
[17]吕莹.牛英鹏运动性疲劳的机制.河南大学学报(自然科学版),2000,30(3)：61-64.
[18]候香玉.运动对自由基代谢的影响[J].体育科学,1991(4)：19-20.
[19]Davies JJA.Free radicals and tissue damage produced by exercise[J]. ApplPhysiol,1982(107):1198-1205.
[20]Brady PS. Selenium,Vita min E and the response to swimming stress in rat[J], Nutr,1997,109:1103-1109.
[21]李峰杨维益,梁嵘等.从中医学看肝脏调节应激反应的作用[J].北京中医药大学学,1998,21(1)：20-22.
[22]王振玉.鹿花盘注射液研究[J].生化药物杂志,1987,(2)：10-11.
[23]张宝香,赵延平.鹿角盘的化学成分与开发利用[J].特种经济动植物,2005,(18)：7.
[24]江苏新医学院.中药大辞典[M].上海：上海科学技术出版社,1986：2230-2231.
[25]李泽鸿,武丽敏,姚玉霞等.梅花鹿鹿茸不同产品中氨基酸含量的比较[J].氨基酸和生物资源,2007,29(3)：16.
[26]苏凤艳,李慧萍,王艳梅等.鹿花盘蛋白质的提取与生物活性测定[J].动物科学与动物医学,2001,18(2)：18-20.
[27]王志兵,邱芳萍,李志民等.鹿角盘活性成分对小鼠吞噬功能及对大鼠乳腺增生激素水平的影响[J].食品科学,2007,31(11)：225-226.
[28]王斌,张声华,李晓莉等.人参总皂甙的耐缺氧效应机理研究[J].食品科学,2002,23(8)：270-272.
[29]VOCES J, ALVAREZ A I,VILAL,et al. Effects of administration of the standardized panax ginseng extract G115 on hepatic antioxidant function after exhaustive exercise [J].Comp Biochem Physiol C Pharmacol Toxicol Endocrinol,1999,123(2):175-184.
[30]Nishibes. Phenolic compounds from stembark of Acanthopanax semicosus and their pharmacologtal effect inchwnic suirmmng stesseel rats[J]. Chem Pharm Bull,1990,38(6):1763.
[31]王庭欣,赵文,刘峥颢.西洋参片对小鼠抗疲劳作用的实验研究[J].食品科学,2005,26(9)：474-475.
[32]罗琼,阎俊,张声华.枸杞多糖对小鼠抗疲劳作用的影响[J].湖北医科大学学报,1999,20(4)：265-269.
[33]李国莉.枸杞多糖对小鼠耐力及体内抗氧化酶活性的影响[J].辽宁中医杂志,2002,29(10)：634.
[34]陈亚东,田长有.高山红景天对小鼠耐缺氧、抗疲劳及耐低温作用的影响[J].中国中医药科技,2002,9(3)：158.
[35]尹旭辉,杨成君,姜在福等.红景天对寒冷适应过程中正常人体免疫功能的调节作用[J].解放军预防医学杂志,1996,14(6)：405.
[36]龚梦娟,刘新民,王立为.肉苁蓉对阳虚小鼠抗疲劳作用的研究[J].中医药导报,2007,13(11)：8-10.
[37]杨毅,贾力莉,武玉鹏等.人工冬虫夏草抗疲劳、降血脂作用实验研究[J].陕西中医,2008,24(10)：46-48.
[38]梁俊荣,张西臣,柳增善等.几种食药用真菌的抗疲劳作用研究[J].中国农业通报,2004,20(1)：135-137.
[39]梅学仁等.灵芝对血管系统药理作用的研究[J].中草药,1982,13(7).
[40]王满福,胡淑萍,李健康等.灵芝液对运动员抗疲劳效果的实验观察[J].中国运动医学杂志,1999,18(1)：78-79.
[41]杨娟,吴谋成,张声华等.香菇蛋白多糖抗疲劳作用研究[J].营养学报,2001,23(4)：350-353.
[42]涂宏海,王彩云,谢玲等.三康胶囊抗疲劳作用及其机理的实验研究[J].解放军药学学报,2006,26(3)：174-176.
[43]王琳,王天芳,张翠珍等.消疲怡神口服液抗应激的实验研究—对强制冷水游泳大鼠行为及神经内分泌的影响[J].北京中医药大学学报,1999,22(3)：22-24.
[44]来英,严卫星,楼密密.保健食品抗疲劳作用试验方法研究[J].中国食品卫生1997,9(4)：1-6,24.
[45]郑建仙.功能性食品(第三卷).北京：中国轻工业出版社,1999
[46]何来英,严卫星,楼密密等.保健食品抗疲劳作用试验方法研究.中国食品卫生杂志,1997,9(4)：1荀,24
[47]毛跟年,许牡丹主编.功能食品生理特性与检测技术[M].北京：化学土业出版社,2005：3-9,351-383,665-666.
[48]邱芳萍,马波,王志兵等.鹿花盘蛋白的分离纯化与活性研究[J].长春工业大学学报,2007,28(3)：144-147.
[49]柴晓杰,崔喜艳.生物化学实验技术[M].吉林：吉林大学出版社,2003,8：59-60.
[50]LaemmLi U K.Nature,1970(227):680.
[51]国家药典委员会.中华人民共和国药典(2005版,一部)[M].北京：化学工业出版社,2005：225-226.
[52]刘景圣,孟先军主编.功能性食品[M].北京：中国农业出版社,2005：252-253.
[53]Naka tani A Han DH, Hansen PA, etal.Effect endurance exercise training on muscle glyocogen super compensation in rats.J A p pl:Physiol,1999,82 (2):711.
[54]万劲.中医补肾药、补脾药对运动员机能状态及运动能力影响的综合观察[J].中国运动医学杂志1994,13(4)：202-206.
[55]潘珊珊,张炎,陆爱云.中医药抗运动性疲劳的研究综述[J].上海体育学院学报,1999,23(2)：52-55.
[56]马莉.红景天苷抗疲劳作用及其机制的实验研究[D].第二军事医学,2006,7.
[57]钱风雷.补肾中药对大鼠运动性低血睾酮的调整作用[J].中国运动医学杂志,1998(4)：230.
[58]傅晓雪.黄芪的香菇发酵及发酵液免疫效果的研究[D].江南大学,2009,5.
[59]张雪香,张桂英,王宝贵.西洋参人参皂甙抗疲劳作用的实验研究[J].中国公共卫生学报,1998,17(4)：238-239
[60]黄增艳,范书铎,库页红景天对鼠抗疲劳作用机理的实验研究[J].中国医科大学学报,1998,27(2)：123-124.
[61]刘声远.高山红景天根茎中超氧化歧化酶活性测定及其种类鉴[J].1993,24(3)：125.
[62]李翼,柴剑波,赵伟国.西洋参抗疲劳作用及对迟发型超敏感反应单核吞噬细胞功能影响的实验研究[J].中华中医药学报,2007,25(10)：2002-2003.
[63]李基洪,孙昌波,彭培勇.冰淇淋生产工艺与配方[M].北京：中国轻工业出版社,2001：356-365.