不同类型土壤纤毛虫分类学与分子系统发育研究
摘要
土壤纤毛虫是土壤生态系统中的重要组成部分,位于异养真核生物网的底层。对其多样性的研究是研究纤毛虫对环境变化指示作用的基础。本工作对东营、昌邑等地的不同类型盐碱土壤中的纤毛虫通过非淹没培养皿法、活体观察、蛋白银染色等方法进行的形态学及纤毛图式等进行了研究,以及对其中的一些种类进行了SSU rDNA测序及系统发育研究,共分离、鉴定了16种纤毛虫。
主要内容包括以下三个方面:
(1)描述了7新种:多变双列虫(Bistichella variabilis),三列沙特虫(Saudithrix tristichella),排伪瘦尾虫(Uroleptoides stichus),中华腹柱虫(Gastrostyla sinensis),四核腹柱虫(Gastrostyla quadrinucleata),尾毛戴维虫(Deviata caudicirrata),双核片尾虫(Urosoma binucleata)。并包括了其中4种的发生过程中的某些阶段。
(2)提取了以下8种土壤纤毛虫的DNA,并对它们的SSU rDNA进行了测序及系统发育分析:多变双列虫(Bistichella variabilis),三列沙特虫(Saudithrix tristichella ),颗粒半小双虫( Hemiamphisiella granulifera ),排伪瘦尾虫(Uroleptoides stichus),中华腹柱虫(Gastrostyla sinensis),四核腹柱虫(Gastrostyla quadrinucleata),尾毛斜裂虫(Deviata caudicirrata),敏捷拟片尾虫(Urosomoida agilis)。
(3)依据所采集到的样品,对9个已知的种类依据统计数据进行了补充描述并与相近种进行了对比:长颗粒尖毛虫(Oxytricha longigranulosa),变藓棘毛虫(Sterkiella histriomuscorum),敏捷拟片尾虫(Urosomoida agilis),颗粒半小双虫(Hemiamphisiella granulifera),近缘殖口虫(Gonostomum affine),拟殖口殖口虫(Gonostomum gonostomoidum),澳洲支柱虫(Cladotricha australis),陆生无柱虫(Eschaneustyla terricola),六列水滴伪康纤虫(Pseudocohnilembus persalinus hexakineta)。
Soil ciliates are at the base of the heterotrophic eukaryotic food web and an essential component in soil ecosystems. Studies of their diversity are the bases for assessing and monitoring environmental changes using ciliates. In this study ciliates from different saline soils from Dongying, Changyi, and some other place were studied morphologically using living observation and protargol impregnation. And SSU rDNA phylogenies of some of these species were studied. The results of identification, classification and phylogenies of these species are summarized as
follows:
(1) Seven new species: Bistichella variabilis, Saudithrix tristichella, Uroleptoides stichus, Gastrostyla sinensis, Gastrostyla quadrinucleata, Deviata caudicirrata, Urosoma binucleata.
(2) SSU rDNA sequences of 8 species were acquired and analyzed: Bistichella variabilis, Saudithrix tristichella, Hemiamphisiella granulifera, Uroleptoides stichus, Gastrostyla sinensis, Gastrostyla quadrinucleata, Deviata caudicirrata, Urosomoida agilis.
(3) 9 species were redescribed based on the new populations: Oxytricha longigranulosa, Sterkiella histriomuscorum, Urosomoida agilis, Hemiamphisiella granulifera, Gonostomum affine, Gonostomum gonostomoidum, Cladotricha australis, Eschaneustyla terricola, Pseudocohnilembus persalinus hexakineta.
主要内容包括以下三个方面:
(1)描述了7新种:多变双列虫(Bistichella variabilis),三列沙特虫(Saudithrix tristichella),排伪瘦尾虫(Uroleptoides stichus),中华腹柱虫(Gastrostyla sinensis),四核腹柱虫(Gastrostyla quadrinucleata),尾毛戴维虫(Deviata caudicirrata),双核片尾虫(Urosoma binucleata)。并包括了其中4种的发生过程中的某些阶段。
(2)提取了以下8种土壤纤毛虫的DNA,并对它们的SSU rDNA进行了测序及系统发育分析:多变双列虫(Bistichella variabilis),三列沙特虫(Saudithrix tristichella ),颗粒半小双虫( Hemiamphisiella granulifera ),排伪瘦尾虫(Uroleptoides stichus),中华腹柱虫(Gastrostyla sinensis),四核腹柱虫(Gastrostyla quadrinucleata),尾毛斜裂虫(Deviata caudicirrata),敏捷拟片尾虫(Urosomoida agilis)。
(3)依据所采集到的样品,对9个已知的种类依据统计数据进行了补充描述并与相近种进行了对比:长颗粒尖毛虫(Oxytricha longigranulosa),变藓棘毛虫(Sterkiella histriomuscorum),敏捷拟片尾虫(Urosomoida agilis),颗粒半小双虫(Hemiamphisiella granulifera),近缘殖口虫(Gonostomum affine),拟殖口殖口虫(Gonostomum gonostomoidum),澳洲支柱虫(Cladotricha australis),陆生无柱虫(Eschaneustyla terricola),六列水滴伪康纤虫(Pseudocohnilembus persalinus hexakineta)。
Soil ciliates are at the base of the heterotrophic eukaryotic food web and an essential component in soil ecosystems. Studies of their diversity are the bases for assessing and monitoring environmental changes using ciliates. In this study ciliates from different saline soils from Dongying, Changyi, and some other place were studied morphologically using living observation and protargol impregnation. And SSU rDNA phylogenies of some of these species were studied. The results of identification, classification and phylogenies of these species are summarized as
follows:
(1) Seven new species: Bistichella variabilis, Saudithrix tristichella, Uroleptoides stichus, Gastrostyla sinensis, Gastrostyla quadrinucleata, Deviata caudicirrata, Urosoma binucleata.
(2) SSU rDNA sequences of 8 species were acquired and analyzed: Bistichella variabilis, Saudithrix tristichella, Hemiamphisiella granulifera, Uroleptoides stichus, Gastrostyla sinensis, Gastrostyla quadrinucleata, Deviata caudicirrata, Urosomoida agilis.
(3) 9 species were redescribed based on the new populations: Oxytricha longigranulosa, Sterkiella histriomuscorum, Urosomoida agilis, Hemiamphisiella granulifera, Gonostomum affine, Gonostomum gonostomoidum, Cladotricha australis, Eschaneustyla terricola, Pseudocohnilembus persalinus hexakineta.
引文
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