外源ABA对干旱胁迫下甘蔗幼苗越冬期间生理生化的影响
摘要
为对重要的植物抗逆调节激素ABA在甘蔗抗旱化学调控中的应用提供理论根据,本试验以品种“粤糖86/368”3叶一心期幼苗为材料,20%PEG-6000创造干旱胁迫条件,7h后叶面喷施不同浓度ABA,然后定期对+1叶进行生理生化指标的测定分析,结果如下:
1、150mg·L~(-1)外源ABA使植株SOD活性比CK平均提高43.3%、POD活性平均提高34.6%。同时降低了自由水/束缚水比值,抑制了叶细胞MDA积累,增强了膜的稳定性,100mg·L~(-1)ABA使膜透性比CK平均下降24.5%。
2、150mg·L~(-1)外源ABA使植株叶绿素a平均提高28.6%、叶绿素b的含量平均提高67.8%和净光合速率平均提高33.1%。
3、150mg·L~(-1)外源ABA使植株可溶性蛋白质的含量平均提高了36.6%;100mg·L~(-1)浓度的ABA使硝酸还原酶的活性提高最显著,平均增强了48.6%。
4、150mg·L~(-1)外源ABA使植株的脯氨酸含量提高最显著,平均提高了21.2%;200mg·L~(-1)使植株的可溶性糖含量提高最显著,平均提高23.1%。
5、150mg·L~(-1)外源ABA使植株的内源ABA含量提高了31.2%,内源IAA含量提高38.5%,内源GA_3含量提高45.8%;100mg·L~(-1)外源ABA使内源ZR含量提高最显著,达47.3%。
6、株高和青叶率均比对照显著提高,且ABA浓度为150mg·L~(-1)时植株生长最好,株高和青叶率分别较对照平均提高了38.6%和33.4%。
结论:ABA有利于提高甘蔗抗旱性,有利于甘蔗幼苗在越冬期干旱胁迫下的生长。ABA浓度为150mg·L~(-1)左右时植株的抗旱性得到最大的提高。
以上实验结果经SPSS方差分析皆达显著水平。
"Guangdong sugar 86/368" was selected as the material,20%PEG-6000 was used to create the conditions of drought stress at the stage of three leaves, sprayed ABA with different concentrations after 7 hours,the first leaf was taken to analyze the Physiological and Biochemical Characters at different times in order to provide theory base for ABA application in sugarcane drought control
1 SOD activity increased 43.3%by 150mg·L~(-1)Exogenous ABA in sugarcane plant,and POD increased 34.6%.At the same time reduce t he ratio of free water/fetter water,inhibited the accumulation of MD A in cells and enhanced the stability of the embrane,the permeability of the membrane decreased 24.5%than CK by 100 mg·L~(-1)ABA.
2 The average concentration of chlorophyll-a increased 28.6%,chlo rophyll-b increased by 33.1%and photosynthetic rate increased by 43.8 %using 150mg·L~(-1)exogenous ABA.
3 The concentration of soluble protein in sugarcane plant increased by36.6%using150mg·L~(-1)of ABA;the activity of nitrate reductase ABA increased by 48.6%using 100 mg·L~(-1)
4 The concentration of proline increased by 21.2%using 150mg·L~(-1) of ABA,plant to the concentration of soluble sugar increased by23.1% using 200 mg·L~(-1)
5 The endogenesis hormone in sugarcane plant were increase greatl y by using 150mg.L-1 of ABA,the concentration of ABA increased 31 .2%,the concentration of IAA increased 38.5%in,the concentration of GA3 increased 45.8%;the content of ZR increased by 47.3%using 1 00 mg·L~(-1)ABA
6 Green leaf rate and plant height improved significantly then the control and the concentration of 150 mg.L~(-1)ABA,increased plant height 38.6%and green leaf rate 33.4%than the control.
Conclusion:ABA can improve the resistance to drought of sugarcane, ABA is conducive to sugarcane growth under drought stress in winter.,the drought-resistance can be increased greatly under the Concentration of ABA 150 mg·L~(-1).
1、150mg·L~(-1)外源ABA使植株SOD活性比CK平均提高43.3%、POD活性平均提高34.6%。同时降低了自由水/束缚水比值,抑制了叶细胞MDA积累,增强了膜的稳定性,100mg·L~(-1)ABA使膜透性比CK平均下降24.5%。
2、150mg·L~(-1)外源ABA使植株叶绿素a平均提高28.6%、叶绿素b的含量平均提高67.8%和净光合速率平均提高33.1%。
3、150mg·L~(-1)外源ABA使植株可溶性蛋白质的含量平均提高了36.6%;100mg·L~(-1)浓度的ABA使硝酸还原酶的活性提高最显著,平均增强了48.6%。
4、150mg·L~(-1)外源ABA使植株的脯氨酸含量提高最显著,平均提高了21.2%;200mg·L~(-1)使植株的可溶性糖含量提高最显著,平均提高23.1%。
5、150mg·L~(-1)外源ABA使植株的内源ABA含量提高了31.2%,内源IAA含量提高38.5%,内源GA_3含量提高45.8%;100mg·L~(-1)外源ABA使内源ZR含量提高最显著,达47.3%。
6、株高和青叶率均比对照显著提高,且ABA浓度为150mg·L~(-1)时植株生长最好,株高和青叶率分别较对照平均提高了38.6%和33.4%。
结论:ABA有利于提高甘蔗抗旱性,有利于甘蔗幼苗在越冬期干旱胁迫下的生长。ABA浓度为150mg·L~(-1)左右时植株的抗旱性得到最大的提高。
以上实验结果经SPSS方差分析皆达显著水平。
"Guangdong sugar 86/368" was selected as the material,20%PEG-6000 was used to create the conditions of drought stress at the stage of three leaves, sprayed ABA with different concentrations after 7 hours,the first leaf was taken to analyze the Physiological and Biochemical Characters at different times in order to provide theory base for ABA application in sugarcane drought control
1 SOD activity increased 43.3%by 150mg·L~(-1)Exogenous ABA in sugarcane plant,and POD increased 34.6%.At the same time reduce t he ratio of free water/fetter water,inhibited the accumulation of MD A in cells and enhanced the stability of the embrane,the permeability of the membrane decreased 24.5%than CK by 100 mg·L~(-1)ABA.
2 The average concentration of chlorophyll-a increased 28.6%,chlo rophyll-b increased by 33.1%and photosynthetic rate increased by 43.8 %using 150mg·L~(-1)exogenous ABA.
3 The concentration of soluble protein in sugarcane plant increased by36.6%using150mg·L~(-1)of ABA;the activity of nitrate reductase ABA increased by 48.6%using 100 mg·L~(-1)
4 The concentration of proline increased by 21.2%using 150mg·L~(-1) of ABA,plant to the concentration of soluble sugar increased by23.1% using 200 mg·L~(-1)
5 The endogenesis hormone in sugarcane plant were increase greatl y by using 150mg.L-1 of ABA,the concentration of ABA increased 31 .2%,the concentration of IAA increased 38.5%in,the concentration of GA3 increased 45.8%;the content of ZR increased by 47.3%using 1 00 mg·L~(-1)ABA
6 Green leaf rate and plant height improved significantly then the control and the concentration of 150 mg.L~(-1)ABA,increased plant height 38.6%and green leaf rate 33.4%than the control.
Conclusion:ABA can improve the resistance to drought of sugarcane, ABA is conducive to sugarcane growth under drought stress in winter.,the drought-resistance can be increased greatly under the Concentration of ABA 150 mg·L~(-1).
引文
[1]曹慧.水分胁迫下对苹果属植物叶片叶绿素降解的膜脂过氧化损伤作用.中国农业科学,2003.36:1191-1195
[2]陈立松,刘星辉.果树对水分胁迫的反应与适应性.干旱地区农业研究,1999,17(1):88-94
[3]陈玲,宋松泉,傅家瑞.番茄种子萌发的高温耐性诱导.种子,1998,5:10-12
[4]陈如凯,张木清,陆裔波.甘蔗品种的御早性差异研究.甘蔗,1996,3(1):1-4
[5]陈如剀,张木清,陆裔波等.干旱胁迫对甘蔗生理影响的研究.甘蔗,1995,2(1):25-28
[6]陈善福,舒庆尧.植物耐干旱胁迫的生物学机理及其基因工程研究进展.植物学通报,1999,16(5):555-560
[7]陈少裕,陈如凯,陈启锋,周可涌.自由基清除剂的保护作用与甘蔗的抗旱性.作物学报,1994,20(2):149-155
[8]陈少裕.膜脂过氧化与植物逆境胁迫.植物学通报,1989,6(4):211-217
[9]从日春,胡雅君,刘洪庆.几种攀援植物耐早性研究.内蒙古林学院学报(自然科学版),1996,3:33-39
[10]付芳靖,赵致,张卫星.水分胁迫下玉米抗旱性与光合生理指标研究.山地农业生物学报,2004,23(6):471-474
[11]高三基,罗俊,陈如凯,张木清,潘大仁.甘蔗品种抗旱性光合生理指标及其综合评价.作物学报,2002,28(1):94-98
[12]谷俊涛,屈平,刘桂茹,等.不同小麦品种抗旱机制与花期旗叶光合特性的关系.华北农学报,2002,17(1):1-5
[13]郭连旺,沈允钢.高等植物光合机构避免强光破坏的保护机构.植物生理学通讯,1996,32(1):1-8
[14]郭卫东,沈向,李嘉瑞等.植物抗旱分子机理.西北农业大学学报,1999,27(4):102-108
[15]胡荣海.农作物抗旱性鉴定方法和指标.作物品种资源,1986,4:36-38
[16]黄有总,徐建云,陈超君等.几个甘蔗新品种的抗旱性和抗寒性比较研究.广西农业生物科学,2002,2l(2):101-104
[17]霍润丰,徐建云,李端富.甘蔗栽培学.广西农学院,1991.10:21-24
[18]简令成.生物膜与植物抗寒害和抗寒性的关系.植物学通报,1983,1(1):17-23
[19]蒋明义.水分胁迫下植物体内HO~-的产生与细胞的氧化损伤.植物学报,1999,41:229-234
[20]金善宝.中国小麦学.北京:中国农业出版社,1996.23:754-758
[21]景蕊莲.作物抗旱研究的现状与思考.干旱地区农业研究,1999,17(2):79-85
[22]康国章,王正询,孙谷畴.几种外源物质提高植物抗冷力的生理机制.植物生理学通讯,2002,38(2):193-197
[23]康绍忠.新农业科技革命与21世纪我国节水农业的发展.干旱地区农业研究,1998,16(1):111-117
[24]李德全,郭清福,张以勤,等.冬小麦抗旱性生理特性的研究.作物学报,1993,11(2):125-131
[25]李德全.土壤水分胁迫下叶片的渗透调节与膨压维持.华北农学报,1991,6(4):100-105
[26]李晚忱,付风玲,袁佐清.苗期耐旱性鉴定方法研究.西南农业大学,2001,14(3):29-32
[27]李杏普.小麦遗传资源研究.北京:中国农业大学出版社,1997.4:45-63
[28]李杨瑞,杨丽涛,陆国盈,梁和.甘蔗品种台糖172的抗性研究综述.广西农学报,1993,(3):25-29
[29]李杨瑞,杨丽涛.论提高蔗糖生产效益的农业措施.甘蔗,1999,6(2):39-42
[30]李杨瑞,杨丽涛.台糖172的抗旱特性研究.广西甘蔗,1991,5(4):9-12
[31]李永健,杨丽涛,李杨瑞,叶燕萍.不同时期喷施乙烯利对甘蔗生长、主要农艺性状及抗旱性的影响.甘蔗,2002,9(1):12-18
[32]李原园,李英能,苏人琼.中国农业水危机及其对策.北京:中国国家科学技术委员会农村科技司,1997,1:52-54
[33]梁丽琼,谭裕模,曾洁清,甘海鹏,梁朝旭,庞天.甘蔗叶片生态特征及脯氨酸含量,膜透性与抗旱性研究.甘蔗,1995,2(4):14-19
[34]廖芬,林鉴钊,李杨瑞,杨丽涛,叶燕萍,谢华.乙烯利对甘蔗叶片结构的影响及其与抗旱性的关系.西南农业学报,2003,16(2):45-48
[35]刘桂茹,陈秀珍.水分胁迫下小麦叶片渗透调节能力与品种抗旱性的关系.河北农业大学学报,2002,4:25-29
[36]刘殊,廖镜思.水分胁迫对龙眼光合作用的影响.果树科学,1997,14(4):244-247
[37]刘子凡,梁计南,罗明珠,赵轩裕.土壤保水剂对秋植蔗形态生理效应的研究.热带农业科学,2004,24(2):18-22
[38]陆国盈,韩世健,裴铁雄等.不同时期施保水剂对甘蔗抗旱性和产量及品质的影响.广西蔗 糖,2005,5(4):3-7
[39]吕长平,石雪辉等.水分胁迫对草Ⅳ叶片SOD活性及MDA及VC含量影响.湖南农业大学学报,1996,22(5):452-455
[40]吕德彬,杨建平,李莲芝,等.水分胁迫下不同小麦品种抗性反应与产量表现的相关研究.河南农业大学学报,1994,28(3):230-235
[41]罗华建,刘星辉.水分胁迫对批把光合特性的影响.果树科学,1999,16(2):126-130
[42]罗俊,林彦铨,张木清,吕建林,陈如凯,洪伟雄.利用甘蔗叶绿体荧光参数及活性氧代谢等指标评价甘蔗品种的耐旱性.甘蔗,1999,6(3):14-22
[43]罗俊,张木清,林彦铨,张华,陈如凯.甘蔗苗期叶绿素荧光参数与抗旱性关系研究.中国农业科学,2004,37(11):1718-1721
[44]罗明珠,刘子凡,梁计南,魏延明.甘蔗抗旱性与叶片某些生理、生化性状的关系[J].亚热带农业研究,2005.3(1):14-16
[45]莫萍丽,林炎坤,叶燕萍,李杨瑞,施丁寿,杨丽涛.水杨酸和乙烯利对甘蔗抗旱性的影响,第三届广西青年学术年会论文集(自然科学篇).南宁:广西人民出版社,2004.3:492-493
[46]潘东明.水分胁迫与批把叶片SOD活性及脂质过氧化作用.福建农学院学报,1993,22(2):54-57
[47]潘世明.甘蔗抗旱性直接鉴定法的综合评价.福建甘蔗,1995,(1):25-28
[48]孙彩霞.水分胁迫对玉米叶片关键防御酶系活性及其同工酶的影响.玉米科学,2003,11(1):63-66
[49]孙广玉,邹琦,程炳嵩,等.大豆光合速率和气孔导度对水分胁迫的响应.植物学报,1991,33(1):43-49
[50]汤章城.植物对水分胁迫的反应和适应性.植物生理学通讯,1983.3:24-29
[51]王环,胡荣海,昌小平.小麦水分胁迫下小麦地上和地下部分的反应及其抗旱性研究.西北植物学报,1996,16(2):107-115
[52]王少先,彭克勤,萧浪涛等.逆境下ABA的积累及其触发机制.植物生理学通讯,2003,39(5):413-419
[53]王锁民编译.农作物抗旱性及耐热性的简易诊断法一根冠淀粉水解法.国外农学——麦类作物,1990,24(2):40-41
[54]王伟,张枫.ABA对渗透胁迫下玉米幼苗根系渗透调节的影响.作物学报,2002,28(1):121-126
[55]王霞,侯平,尹林克等.水分胁迫对柽柳植物可溶性物质的影响.干旱区研究,1999,16(2):6-11
[56]王晓琴,袁继超,柯永培,熊庆娥.水分胁迫对玉米幼苗氮素代谢的影响.农业大学学报,2004,22(1):23-25
[57]王月福,于振文,李尚霞,余松烈.氮素营养水平对冬小麦氮代谢关键酶活性变化和子粒蛋白质含量的影响.作物学报,2002,28(6):743-748
[58]王自章,张树珍,李杨瑞,林俊芳,罗敬萍,张治礼,陈如凯.干旱诱导型启动子Prd29A的克隆及其序列分析.热带生物学报,2000,21(增刊):178-182
[59]王自章,张树珍,李杨瑞.大肠杆菌海藻糖磷酸合酶基因的克隆.广西农业生物科,2002,(2):98-100
[60]王自章,张树珍,杨本鹏,李杨瑞.甘蔗根癌农杆菌介导转化海藻糖合酶基因获得抗渗透胁迫能力增强植株.中国农业科学,2003,36(2):140-146
[61]吴凯朝,叶燕萍,李杨瑞,李永健,杨丽涛.喷施乙烯利对甘蔗群体冠层结构及一些抗旱性生理指标的影响.西南农业学报,2004,17(6):724-729
[62]谢吉容,向邓云,梅虎等.南方红豆杉抗寒性的变化与内源激素的关系.西南师范大学学报(自然科学版),2002,27(2):231-234
[63]徐世健,安黎哲,冯虎元等.两种沙生植物抗旱生理指标的比较研究.西北植物学报,2000,20(2):224-228
[64]薛亮,聂新鹏,王晋臣.规模化发展——广西蔗糖业生产的必由之路.农业经济问题,2004,2(4):40-43
[65]杨丽涛,李杨瑞.干旱条件下后期灌溉对不同品种甘蔗产量、蔗糖分和某些生理生化特性的效应研究.作物学报,1995,21(1):76-82
[66]杨丽涛,李杨瑞.甘蔗两个抗性生理指标的初步研究.四川甘蔗,1990,(2):6-7
[67]叶燕萍,李杨瑞,李永健,杨丽涛.不同种苗处理方式甘蔗对干旱条件的反应.热带作物学报,2001,22(2):62-67
[68]叶燕萍,李杨瑞,黄诚梅,李永健,邢永秀.下种方式和水分胁迫对甘蔗叶片光合特性的影响.甘蔗,2003,10(3):1-4
[69]叶燕萍,李杨瑞,李永建,黄诚梅,唐军.反复干旱法在甘蔗抗旱性研究中的应用.中国糖料,2003,(3):12-16
[70]叶燕萍,李杨瑞,李永健,黄诚梅.甘蔗三种砍种方式的抗旱性比较研究.广西植物,2002,(2):181-184
[71]叶燕萍,李杨瑞,罗霆.乙烯利浸种对甘蔗抗旱性的影响.中国农学通报,2005,21(6):387-389
[72]叶燕萍,唐军,李杨瑞,李永建,杨丽涛.干旱和灌溉条件下两个甘蔗品种的几个抗旱生理指标研究.中国糖料,2003,(1):1-5
[73]叶燕萍,杨丽涛,李杨瑞.蔗地深耕深松对甘蔗吸收N、P、K及产量品质的影响.甘蔗,1995,2(1):50-51
[74]于同泉,秦岭等.水分胁迫对板栗幼苗抗氧化酶及丙二醛的影响.北京农学院学报,1996,11(1):48-52
[75]张爱军,商振清,董永华,等.6-BA和KT对干旱条件下小麦旗叶甘油醛-3-磷酸脱氢酶及光合作用的影响.河北农业大学学报,2000,23(2):37-41
[76]张林刚,邓西平.小麦抗旱性生理生化研究进展.干旱地区农业研究,2000,18(3):125-132
[77]赵春江,康书江,王纪华等.植物内源激素与不同基因型小麦抗寒性关系的研究.华北农学报,2000,15(3):51-54
[78]钟希琼,林丽超.甘蔗品种抗旱力与生理指标的关系.佛山科学技术学院学报(自然科学版),2002.20:43-45
[79]钟希琼,叶振邦.综合评价甘蔗的抗旱性及抗性生理指标.华南农业大学学报,1995,17(3):36-42
[80]周琼,李杨瑞,林鉴钊,叶燕萍,杨丽涛.喷施乙烯利对甘蔗生长前期甘蔗叶片维管束结构及硅镁锌含量的影响.中国农学通报,2005,21(2):147-149,174
[81]紫守玺.与小麦抗旱性相关的几个水分指标.甘肃农业科技,1990,(6):12-13
[82]邹琦,李德全主编.作物栽培生理研究.中国农业科技出版社,1998
[83]ALSCHER R QDONAHUE 7 L.CRAMER C L Reactive oxygen species and antioxidants:relationship singeren cells.Physiologia Plant,1997,100:224-233
[84]Blackman S A.Maturation proteins and sugars in desiccation tolerance of developing soybean seeds.Plant Physiology,1992,100:225-230
[85]Blum Aetal.Chemical desication of wheat plants as a simulator of postanthesis stree Ⅱ Rel ations to Drought stress.Field Crop Res.1983,6(2):149-155
[86]Bouslama Metal.Stress to learn ceinsoy bean.1.Evaluation of the Screening techniques for heat and drought to lerance.Crop Sci.1984,24:9 33-937
[87]Chen Y.Roles of carbohydrates in desiccation tolerance and membrane behavior in maturing maize seed. Crop Science, 1990,30:971-975
[88] Haley S D. et.Al. Early generation selection for chemical desiccation tolerance winter heat. C-orp Sci. 1993,33(6): 1217-1223
[89] Hwang S Y, Van Toai TT.Abscisic acid induces anaerobiotic tolerance in corn. Plant physiol. 1991,97: 593-597
[90] Koehler P H, Moore P H, Jones A. Response of drip-irrigated sugarcane to drought stress. Agonomy, 1982,74:906-911
[91] Li Y R, Liao W Z, Yang L T, L in Y K, Nong Y Y and Liu Y. Improving ripening and dr-ought resistancein sugarcane by application of ethephon.Li YR and Solomon S. (Editors in Chief). Sustainable sugarcane and sugar production technology. Beijing: China Agric-ulture Press, 2004. 374-377
[92] Li Y R, Solomon S. Ethephon: a versatile growth regulator for sugarcane industry. Sug-ar Tech, 2003, 5(4): 213-223
[93] Luo R H, L i Y R, L in Y K. Effects of foliar spray of ethephon at tillering stage on mesoph-yll cells and chloroplasts in sugarcane. Li YR and Solomon S. (Editors in Chief). Sustai-nable Sugarcane and Sugar Production Technology.Beijing: China Agriculture Press, 2004. 350-352
[94] Regan. K. L. et al. Evaluation of chemical desiccationa selection technique for drought resi-stance in dry land wheat breeding program. Aust, J. AgricRes, 1993,44: 1683-1691
[95] Ren HuiBo,FANYiJian, et al.,Roles of a sustained activation of NCED3 and the synergistic regul-ation of ABA biosynthesis and catabolism in ABA signal production in Arabidopsis
[96] Salin ML. To xic Oxygen Specis and Protective System of the Chloroplast. Physiol Plant, 1988, 25(3): 41-246
[97] Scinchezdiaz M F, Krammer P J. urgor diferences and water stress in maize and sorghum leaves during drought drecovery. ExP.Bot. 1984,88: 511-515
[98] Teulat B. et al. Relationship between relative water content and growth parameters under w ater s-tress in barley: a Q TL study. New Phytol. 1997,137: 99-107
[99] TeulatB. et al. Several Q T Ls involved in osmoitcad justment Trait variation in barley (Hordeum vulgare L). Theory Apple Genet. 1998,96: 688-698
[100] Winter S. R. Evalustion of screening technique for breeding drought-resistance winter wheat. Crop Sci. 1988,28(3): 526-531
[101]Yao J Y,Yang L T,Fang W K,Li Y R.St imulatory effects of ethephon on photo synthesis of sugarcane under water deficit conditions.In Li Y R and Solomon S.(Ed-itor s in Chief).Sustainable Sugarcane and Sugar Production Technology.Beijing:China Agricu lture Press,2004.384-387
[102]Yordanor I,Tsonev T,Goltsev V,et al Gas exchange and chlorophyⅡ fluorescence d -uring water and high temperature stresses and recovery.Photosynthetca,1997,33:423-431
[2]陈立松,刘星辉.果树对水分胁迫的反应与适应性.干旱地区农业研究,1999,17(1):88-94
[3]陈玲,宋松泉,傅家瑞.番茄种子萌发的高温耐性诱导.种子,1998,5:10-12
[4]陈如凯,张木清,陆裔波.甘蔗品种的御早性差异研究.甘蔗,1996,3(1):1-4
[5]陈如剀,张木清,陆裔波等.干旱胁迫对甘蔗生理影响的研究.甘蔗,1995,2(1):25-28
[6]陈善福,舒庆尧.植物耐干旱胁迫的生物学机理及其基因工程研究进展.植物学通报,1999,16(5):555-560
[7]陈少裕,陈如凯,陈启锋,周可涌.自由基清除剂的保护作用与甘蔗的抗旱性.作物学报,1994,20(2):149-155
[8]陈少裕.膜脂过氧化与植物逆境胁迫.植物学通报,1989,6(4):211-217
[9]从日春,胡雅君,刘洪庆.几种攀援植物耐早性研究.内蒙古林学院学报(自然科学版),1996,3:33-39
[10]付芳靖,赵致,张卫星.水分胁迫下玉米抗旱性与光合生理指标研究.山地农业生物学报,2004,23(6):471-474
[11]高三基,罗俊,陈如凯,张木清,潘大仁.甘蔗品种抗旱性光合生理指标及其综合评价.作物学报,2002,28(1):94-98
[12]谷俊涛,屈平,刘桂茹,等.不同小麦品种抗旱机制与花期旗叶光合特性的关系.华北农学报,2002,17(1):1-5
[13]郭连旺,沈允钢.高等植物光合机构避免强光破坏的保护机构.植物生理学通讯,1996,32(1):1-8
[14]郭卫东,沈向,李嘉瑞等.植物抗旱分子机理.西北农业大学学报,1999,27(4):102-108
[15]胡荣海.农作物抗旱性鉴定方法和指标.作物品种资源,1986,4:36-38
[16]黄有总,徐建云,陈超君等.几个甘蔗新品种的抗旱性和抗寒性比较研究.广西农业生物科学,2002,2l(2):101-104
[17]霍润丰,徐建云,李端富.甘蔗栽培学.广西农学院,1991.10:21-24
[18]简令成.生物膜与植物抗寒害和抗寒性的关系.植物学通报,1983,1(1):17-23
[19]蒋明义.水分胁迫下植物体内HO~-的产生与细胞的氧化损伤.植物学报,1999,41:229-234
[20]金善宝.中国小麦学.北京:中国农业出版社,1996.23:754-758
[21]景蕊莲.作物抗旱研究的现状与思考.干旱地区农业研究,1999,17(2):79-85
[22]康国章,王正询,孙谷畴.几种外源物质提高植物抗冷力的生理机制.植物生理学通讯,2002,38(2):193-197
[23]康绍忠.新农业科技革命与21世纪我国节水农业的发展.干旱地区农业研究,1998,16(1):111-117
[24]李德全,郭清福,张以勤,等.冬小麦抗旱性生理特性的研究.作物学报,1993,11(2):125-131
[25]李德全.土壤水分胁迫下叶片的渗透调节与膨压维持.华北农学报,1991,6(4):100-105
[26]李晚忱,付风玲,袁佐清.苗期耐旱性鉴定方法研究.西南农业大学,2001,14(3):29-32
[27]李杏普.小麦遗传资源研究.北京:中国农业大学出版社,1997.4:45-63
[28]李杨瑞,杨丽涛,陆国盈,梁和.甘蔗品种台糖172的抗性研究综述.广西农学报,1993,(3):25-29
[29]李杨瑞,杨丽涛.论提高蔗糖生产效益的农业措施.甘蔗,1999,6(2):39-42
[30]李杨瑞,杨丽涛.台糖172的抗旱特性研究.广西甘蔗,1991,5(4):9-12
[31]李永健,杨丽涛,李杨瑞,叶燕萍.不同时期喷施乙烯利对甘蔗生长、主要农艺性状及抗旱性的影响.甘蔗,2002,9(1):12-18
[32]李原园,李英能,苏人琼.中国农业水危机及其对策.北京:中国国家科学技术委员会农村科技司,1997,1:52-54
[33]梁丽琼,谭裕模,曾洁清,甘海鹏,梁朝旭,庞天.甘蔗叶片生态特征及脯氨酸含量,膜透性与抗旱性研究.甘蔗,1995,2(4):14-19
[34]廖芬,林鉴钊,李杨瑞,杨丽涛,叶燕萍,谢华.乙烯利对甘蔗叶片结构的影响及其与抗旱性的关系.西南农业学报,2003,16(2):45-48
[35]刘桂茹,陈秀珍.水分胁迫下小麦叶片渗透调节能力与品种抗旱性的关系.河北农业大学学报,2002,4:25-29
[36]刘殊,廖镜思.水分胁迫对龙眼光合作用的影响.果树科学,1997,14(4):244-247
[37]刘子凡,梁计南,罗明珠,赵轩裕.土壤保水剂对秋植蔗形态生理效应的研究.热带农业科学,2004,24(2):18-22
[38]陆国盈,韩世健,裴铁雄等.不同时期施保水剂对甘蔗抗旱性和产量及品质的影响.广西蔗 糖,2005,5(4):3-7
[39]吕长平,石雪辉等.水分胁迫对草Ⅳ叶片SOD活性及MDA及VC含量影响.湖南农业大学学报,1996,22(5):452-455
[40]吕德彬,杨建平,李莲芝,等.水分胁迫下不同小麦品种抗性反应与产量表现的相关研究.河南农业大学学报,1994,28(3):230-235
[41]罗华建,刘星辉.水分胁迫对批把光合特性的影响.果树科学,1999,16(2):126-130
[42]罗俊,林彦铨,张木清,吕建林,陈如凯,洪伟雄.利用甘蔗叶绿体荧光参数及活性氧代谢等指标评价甘蔗品种的耐旱性.甘蔗,1999,6(3):14-22
[43]罗俊,张木清,林彦铨,张华,陈如凯.甘蔗苗期叶绿素荧光参数与抗旱性关系研究.中国农业科学,2004,37(11):1718-1721
[44]罗明珠,刘子凡,梁计南,魏延明.甘蔗抗旱性与叶片某些生理、生化性状的关系[J].亚热带农业研究,2005.3(1):14-16
[45]莫萍丽,林炎坤,叶燕萍,李杨瑞,施丁寿,杨丽涛.水杨酸和乙烯利对甘蔗抗旱性的影响,第三届广西青年学术年会论文集(自然科学篇).南宁:广西人民出版社,2004.3:492-493
[46]潘东明.水分胁迫与批把叶片SOD活性及脂质过氧化作用.福建农学院学报,1993,22(2):54-57
[47]潘世明.甘蔗抗旱性直接鉴定法的综合评价.福建甘蔗,1995,(1):25-28
[48]孙彩霞.水分胁迫对玉米叶片关键防御酶系活性及其同工酶的影响.玉米科学,2003,11(1):63-66
[49]孙广玉,邹琦,程炳嵩,等.大豆光合速率和气孔导度对水分胁迫的响应.植物学报,1991,33(1):43-49
[50]汤章城.植物对水分胁迫的反应和适应性.植物生理学通讯,1983.3:24-29
[51]王环,胡荣海,昌小平.小麦水分胁迫下小麦地上和地下部分的反应及其抗旱性研究.西北植物学报,1996,16(2):107-115
[52]王少先,彭克勤,萧浪涛等.逆境下ABA的积累及其触发机制.植物生理学通讯,2003,39(5):413-419
[53]王锁民编译.农作物抗旱性及耐热性的简易诊断法一根冠淀粉水解法.国外农学——麦类作物,1990,24(2):40-41
[54]王伟,张枫.ABA对渗透胁迫下玉米幼苗根系渗透调节的影响.作物学报,2002,28(1):121-126
[55]王霞,侯平,尹林克等.水分胁迫对柽柳植物可溶性物质的影响.干旱区研究,1999,16(2):6-11
[56]王晓琴,袁继超,柯永培,熊庆娥.水分胁迫对玉米幼苗氮素代谢的影响.农业大学学报,2004,22(1):23-25
[57]王月福,于振文,李尚霞,余松烈.氮素营养水平对冬小麦氮代谢关键酶活性变化和子粒蛋白质含量的影响.作物学报,2002,28(6):743-748
[58]王自章,张树珍,李杨瑞,林俊芳,罗敬萍,张治礼,陈如凯.干旱诱导型启动子Prd29A的克隆及其序列分析.热带生物学报,2000,21(增刊):178-182
[59]王自章,张树珍,李杨瑞.大肠杆菌海藻糖磷酸合酶基因的克隆.广西农业生物科,2002,(2):98-100
[60]王自章,张树珍,杨本鹏,李杨瑞.甘蔗根癌农杆菌介导转化海藻糖合酶基因获得抗渗透胁迫能力增强植株.中国农业科学,2003,36(2):140-146
[61]吴凯朝,叶燕萍,李杨瑞,李永健,杨丽涛.喷施乙烯利对甘蔗群体冠层结构及一些抗旱性生理指标的影响.西南农业学报,2004,17(6):724-729
[62]谢吉容,向邓云,梅虎等.南方红豆杉抗寒性的变化与内源激素的关系.西南师范大学学报(自然科学版),2002,27(2):231-234
[63]徐世健,安黎哲,冯虎元等.两种沙生植物抗旱生理指标的比较研究.西北植物学报,2000,20(2):224-228
[64]薛亮,聂新鹏,王晋臣.规模化发展——广西蔗糖业生产的必由之路.农业经济问题,2004,2(4):40-43
[65]杨丽涛,李杨瑞.干旱条件下后期灌溉对不同品种甘蔗产量、蔗糖分和某些生理生化特性的效应研究.作物学报,1995,21(1):76-82
[66]杨丽涛,李杨瑞.甘蔗两个抗性生理指标的初步研究.四川甘蔗,1990,(2):6-7
[67]叶燕萍,李杨瑞,李永健,杨丽涛.不同种苗处理方式甘蔗对干旱条件的反应.热带作物学报,2001,22(2):62-67
[68]叶燕萍,李杨瑞,黄诚梅,李永健,邢永秀.下种方式和水分胁迫对甘蔗叶片光合特性的影响.甘蔗,2003,10(3):1-4
[69]叶燕萍,李杨瑞,李永建,黄诚梅,唐军.反复干旱法在甘蔗抗旱性研究中的应用.中国糖料,2003,(3):12-16
[70]叶燕萍,李杨瑞,李永健,黄诚梅.甘蔗三种砍种方式的抗旱性比较研究.广西植物,2002,(2):181-184
[71]叶燕萍,李杨瑞,罗霆.乙烯利浸种对甘蔗抗旱性的影响.中国农学通报,2005,21(6):387-389
[72]叶燕萍,唐军,李杨瑞,李永建,杨丽涛.干旱和灌溉条件下两个甘蔗品种的几个抗旱生理指标研究.中国糖料,2003,(1):1-5
[73]叶燕萍,杨丽涛,李杨瑞.蔗地深耕深松对甘蔗吸收N、P、K及产量品质的影响.甘蔗,1995,2(1):50-51
[74]于同泉,秦岭等.水分胁迫对板栗幼苗抗氧化酶及丙二醛的影响.北京农学院学报,1996,11(1):48-52
[75]张爱军,商振清,董永华,等.6-BA和KT对干旱条件下小麦旗叶甘油醛-3-磷酸脱氢酶及光合作用的影响.河北农业大学学报,2000,23(2):37-41
[76]张林刚,邓西平.小麦抗旱性生理生化研究进展.干旱地区农业研究,2000,18(3):125-132
[77]赵春江,康书江,王纪华等.植物内源激素与不同基因型小麦抗寒性关系的研究.华北农学报,2000,15(3):51-54
[78]钟希琼,林丽超.甘蔗品种抗旱力与生理指标的关系.佛山科学技术学院学报(自然科学版),2002.20:43-45
[79]钟希琼,叶振邦.综合评价甘蔗的抗旱性及抗性生理指标.华南农业大学学报,1995,17(3):36-42
[80]周琼,李杨瑞,林鉴钊,叶燕萍,杨丽涛.喷施乙烯利对甘蔗生长前期甘蔗叶片维管束结构及硅镁锌含量的影响.中国农学通报,2005,21(2):147-149,174
[81]紫守玺.与小麦抗旱性相关的几个水分指标.甘肃农业科技,1990,(6):12-13
[82]邹琦,李德全主编.作物栽培生理研究.中国农业科技出版社,1998
[83]ALSCHER R QDONAHUE 7 L.CRAMER C L Reactive oxygen species and antioxidants:relationship singeren cells.Physiologia Plant,1997,100:224-233
[84]Blackman S A.Maturation proteins and sugars in desiccation tolerance of developing soybean seeds.Plant Physiology,1992,100:225-230
[85]Blum Aetal.Chemical desication of wheat plants as a simulator of postanthesis stree Ⅱ Rel ations to Drought stress.Field Crop Res.1983,6(2):149-155
[86]Bouslama Metal.Stress to learn ceinsoy bean.1.Evaluation of the Screening techniques for heat and drought to lerance.Crop Sci.1984,24:9 33-937
[87]Chen Y.Roles of carbohydrates in desiccation tolerance and membrane behavior in maturing maize seed. Crop Science, 1990,30:971-975
[88] Haley S D. et.Al. Early generation selection for chemical desiccation tolerance winter heat. C-orp Sci. 1993,33(6): 1217-1223
[89] Hwang S Y, Van Toai TT.Abscisic acid induces anaerobiotic tolerance in corn. Plant physiol. 1991,97: 593-597
[90] Koehler P H, Moore P H, Jones A. Response of drip-irrigated sugarcane to drought stress. Agonomy, 1982,74:906-911
[91] Li Y R, Liao W Z, Yang L T, L in Y K, Nong Y Y and Liu Y. Improving ripening and dr-ought resistancein sugarcane by application of ethephon.Li YR and Solomon S. (Editors in Chief). Sustainable sugarcane and sugar production technology. Beijing: China Agric-ulture Press, 2004. 374-377
[92] Li Y R, Solomon S. Ethephon: a versatile growth regulator for sugarcane industry. Sug-ar Tech, 2003, 5(4): 213-223
[93] Luo R H, L i Y R, L in Y K. Effects of foliar spray of ethephon at tillering stage on mesoph-yll cells and chloroplasts in sugarcane. Li YR and Solomon S. (Editors in Chief). Sustai-nable Sugarcane and Sugar Production Technology.Beijing: China Agriculture Press, 2004. 350-352
[94] Regan. K. L. et al. Evaluation of chemical desiccationa selection technique for drought resi-stance in dry land wheat breeding program. Aust, J. AgricRes, 1993,44: 1683-1691
[95] Ren HuiBo,FANYiJian, et al.,Roles of a sustained activation of NCED3 and the synergistic regul-ation of ABA biosynthesis and catabolism in ABA signal production in Arabidopsis
[96] Salin ML. To xic Oxygen Specis and Protective System of the Chloroplast. Physiol Plant, 1988, 25(3): 41-246
[97] Scinchezdiaz M F, Krammer P J. urgor diferences and water stress in maize and sorghum leaves during drought drecovery. ExP.Bot. 1984,88: 511-515
[98] Teulat B. et al. Relationship between relative water content and growth parameters under w ater s-tress in barley: a Q TL study. New Phytol. 1997,137: 99-107
[99] TeulatB. et al. Several Q T Ls involved in osmoitcad justment Trait variation in barley (Hordeum vulgare L). Theory Apple Genet. 1998,96: 688-698
[100] Winter S. R. Evalustion of screening technique for breeding drought-resistance winter wheat. Crop Sci. 1988,28(3): 526-531
[101]Yao J Y,Yang L T,Fang W K,Li Y R.St imulatory effects of ethephon on photo synthesis of sugarcane under water deficit conditions.In Li Y R and Solomon S.(Ed-itor s in Chief).Sustainable Sugarcane and Sugar Production Technology.Beijing:China Agricu lture Press,2004.384-387
[102]Yordanor I,Tsonev T,Goltsev V,et al Gas exchange and chlorophyⅡ fluorescence d -uring water and high temperature stresses and recovery.Photosynthetca,1997,33:423-431