甜瓜枯萎病苗期接种鉴定体系建立及其应用
|
摘要
甜瓜枯萎病(Fusarium oxysporum f. sp. melonis)是危害甜瓜生产的重要土传病害,在全国各地均有发生。苗期进行枯萎病接种抗性鉴定可对甜瓜品种的抗病性进行快速鉴定,但目前国内尚未建立可靠的甜瓜苗期接种鉴定技术体系,本试验首先对甜瓜苗期枯萎病接种鉴定技术体系进行了研究,对不同甜瓜品种和砧木的抗病性进行鉴定评价,并研究了不同嫁接方法对甜瓜枯萎病的影响,旨在建立甜瓜苗期枯萎病接种鉴定技术体系,对甜瓜和砧木的抗枯萎病能力进行评价,提出适合甜瓜嫁接的方法。
采用蘸根法接种研究了甜瓜枯萎病菌不同孢子接种浓度(1×105、1×106、1×107个·mL-1)和不同接种苗龄(子叶期、2叶1心期、4叶1心期)对甜瓜枯萎病发生的影响。结果表明,采用1×106个·mL-1孢子液浓度,筛选压力适中,病情指数达到70左右,在甜瓜幼苗2叶1心期进行接种鉴定,抗感性差异表现显著,最佳的病害调查时间为接种后第10天,利于节省时间和人力。
在抗病材料筛选试验中,采用前述鉴定方法对18个生产上普遍栽培的甜瓜品种进行枯萎病鉴定,结果表明,新金雪莲、长香玉、玉姑、春辉、蜜绿5个品种高抗甜瓜枯萎病。对18份南瓜砧木进行苗期枯萎病接种鉴定,结果表明所有品种都高抗甜瓜枯萎病。
采用插接、断根嫁接和靠接3种嫁接方法,研究了不同嫁接方法对甜瓜枯萎病抗性的影响。结果表明,嫁接能够显著提高甜瓜的抗枯萎病能力,不同嫁接方法对枯萎病抗性无显著差异。对自根苗和嫁接苗接种枯萎病后的生理代谢进行了研究,结果表明,嫁接苗根系活力、叶绿素、MDA、相对电导率与对照无显著差异,嫁接苗根系中SOD、POD、PAL活性显著高于对照。
Fusarium Wilt(Fusarium oxysporum f. sp. Melonis) is an important soil-borne disease, has occurred throughout China. Inoculated at the seedling stage can be used as a method of quickly wilt resistance identification. At present, a reliable system of melon seedling inoculation technique has not been established. So this study first established and improved the identification system of Fusarium wilt of melon seedlings, and then identified the resistance evaluation and studied different grafting methods on Fusarium wilt of melon.
The disease resistance of melon to Fusarium wilt was identified with different concentrations of pathogen spores(1×105、1×106、1×107 spore·mL-1) at different seeding stages(cotyledon stage,2 true leaves stage and 4 true leaves stage) by root dipping inoculation. The results indicated that the best concentration for inoculation was 1×106 spore·mL-1 at two leaves stage. Because selection pressure was moderate, the disease index in the 70 or so, and the performance of disease susceptibility was significantly different. And the best time for resistance evaluation was 10 days after inoculation, which would help save time and manpower.
The sreening tests of 18 melon common cultivars in production showed that 'Xinjinxuelian','Changxiangyu','Yugu','Chunhui' and 'Milv' had high resistance to melon Fusarium wilt. And the sreening tests of 18 squash cultivars showed that all varieties were highly resistant to Fusarium wilt of melon.
Experiment with different grafting methods, the use of plug, root pruning and dock, showed that grafting significantly improved the ability of resistance to Fusarium wilt of melon. However, different grafting methods on resistance to Fusarium wilt were no significant difference. Through the physiological metabolism of self-rooted and grafted seedlings both inoculated with Fusarium wilt, root activity, chlorophyll, MDA and relative conductivity of grafted plants were no significant difference with the control. SOD, POD, PAL activity in the roots of grafted plants were significantly higher than the control.
采用蘸根法接种研究了甜瓜枯萎病菌不同孢子接种浓度(1×105、1×106、1×107个·mL-1)和不同接种苗龄(子叶期、2叶1心期、4叶1心期)对甜瓜枯萎病发生的影响。结果表明,采用1×106个·mL-1孢子液浓度,筛选压力适中,病情指数达到70左右,在甜瓜幼苗2叶1心期进行接种鉴定,抗感性差异表现显著,最佳的病害调查时间为接种后第10天,利于节省时间和人力。
在抗病材料筛选试验中,采用前述鉴定方法对18个生产上普遍栽培的甜瓜品种进行枯萎病鉴定,结果表明,新金雪莲、长香玉、玉姑、春辉、蜜绿5个品种高抗甜瓜枯萎病。对18份南瓜砧木进行苗期枯萎病接种鉴定,结果表明所有品种都高抗甜瓜枯萎病。
采用插接、断根嫁接和靠接3种嫁接方法,研究了不同嫁接方法对甜瓜枯萎病抗性的影响。结果表明,嫁接能够显著提高甜瓜的抗枯萎病能力,不同嫁接方法对枯萎病抗性无显著差异。对自根苗和嫁接苗接种枯萎病后的生理代谢进行了研究,结果表明,嫁接苗根系活力、叶绿素、MDA、相对电导率与对照无显著差异,嫁接苗根系中SOD、POD、PAL活性显著高于对照。
Fusarium Wilt(Fusarium oxysporum f. sp. Melonis) is an important soil-borne disease, has occurred throughout China. Inoculated at the seedling stage can be used as a method of quickly wilt resistance identification. At present, a reliable system of melon seedling inoculation technique has not been established. So this study first established and improved the identification system of Fusarium wilt of melon seedlings, and then identified the resistance evaluation and studied different grafting methods on Fusarium wilt of melon.
The disease resistance of melon to Fusarium wilt was identified with different concentrations of pathogen spores(1×105、1×106、1×107 spore·mL-1) at different seeding stages(cotyledon stage,2 true leaves stage and 4 true leaves stage) by root dipping inoculation. The results indicated that the best concentration for inoculation was 1×106 spore·mL-1 at two leaves stage. Because selection pressure was moderate, the disease index in the 70 or so, and the performance of disease susceptibility was significantly different. And the best time for resistance evaluation was 10 days after inoculation, which would help save time and manpower.
The sreening tests of 18 melon common cultivars in production showed that 'Xinjinxuelian','Changxiangyu','Yugu','Chunhui' and 'Milv' had high resistance to melon Fusarium wilt. And the sreening tests of 18 squash cultivars showed that all varieties were highly resistant to Fusarium wilt of melon.
Experiment with different grafting methods, the use of plug, root pruning and dock, showed that grafting significantly improved the ability of resistance to Fusarium wilt of melon. However, different grafting methods on resistance to Fusarium wilt were no significant difference. Through the physiological metabolism of self-rooted and grafted seedlings both inoculated with Fusarium wilt, root activity, chlorophyll, MDA and relative conductivity of grafted plants were no significant difference with the control. SOD, POD, PAL activity in the roots of grafted plants were significantly higher than the control.
引文
1 安学君,金伟兴,陆培生,蒋思恒,陈林松,赵海棠.大棚嫁接西瓜砧木新品种筛选试验初报.中国瓜菜,2005(4)：32-34
2 别之龙,朱进,黄远.黄瓜断根嫁接工厂化穴盘育苗技术.中国蔬菜,2006(8)：48-49
3 蔡竹固,陈瑞祥,童伯开.台湾甜瓜蔓割病菌之生理小种.植保会刊,1999,41：139-143
4 陈士海,季海军.嫁接黄瓜枯萎病的发生与防治.植物保护,2010(9),23
5 崔健,宋云云,刘素芹,张淑霞.厚皮甜瓜舌形靠接育苗技术.西北园艺,2008(7)：21-22
6 韩金星,洪日新,周林,何毅,黄金艳,覃斯华,李文信,樊学军,李天艳.西瓜、黄瓜、甜瓜等瓜类枯萎病研究进展.中国瓜菜,2009(2)：32-35
7 吉加兵.西瓜枯萎病苗期抗性鉴定方法的探讨.中国西瓜甜瓜,1992(1)：35-39
8 李金玉,颜雪,黄琼,赵惠新,李冠.甜瓜抗枯萎病基因同源序列克隆与序列分析.生物技术,2006,16(3)：3-9
9 高梅秀,李树和,刘玉芹等.不同砧木对茄子抗病性、生理活性及产量的影响.园艺学报,2001,28(5)：463-465
10顾卫红,宋荣浩.西瓜种质资源主要园艺学性状的评价.上海农学院学报,1996,14(4)：294-299
1 1顾卫红,王鸣.甜瓜种质资源对枯萎病抗性的苗期人工接种鉴定.中国西瓜甜瓜,1989(1)： 1-8
12顾卫红,王鸣.几种氧化酶同工酶与甜瓜抗枯萎病关系研究.北方园艺.1992(2)：7-9
13黄奔立,许云东,张顺琦,陈学好.根系分泌物影响黄瓜枯萎病抗性的机理研究.扬州大学学报,2007,28(3)：77-81
14黄志光,乐秋歆,吴建能.嫁接与非嫁接西瓜抗枯萎病田间试验简报.上海农业科技,2010(2)：91
15蒋有条.西瓜甜瓜的嫁接栽培(综述)中国西瓜甜瓜,1989(1)：27-31
16雷鸣.嫁接对西瓜枯萎病抗性的影响.安徽农业科学,2001,29(5)：655-656
17李光,李淑菊,王惠哲.兼抗黄瓜枯萎病、角斑病和黑星病育种材料苗期筛选.天津农业科学,2007,13(3)：23-25
18李合生.植物生理生化实验原理和技术.北京：高等教育出版社,2000,205-278
19李合生.现代植物生理学.北京：高等教育出版社,2002：505
20李振卓,祁佐宽,邓守哲.不同嫁接方法对薄皮甜瓜生长速度成活率及抗冷性作用研究.农业与技术,2009,29(4)：61-65
21李明远,李兴红,黄金宝,严红,张涛涛,周莹.瓜类枯萎病及其防治.中国蔬菜,2008(9)：57-60
22刘春艳,王万立,郝永娟,王勇.大棚甜瓜枯萎病的发生及综合防治.农业科技通,2010(1)：171-172
23刘文革.甜瓜(Cucumis melo)基因目录.中国西瓜甜瓜,2000(2)：37-40
24刘文革.甜瓜基因目录(2002).中国西瓜甜瓜,2004(4)：44-45
25刘国生.微生物学实验技术.北京：科学出版社,2007
26刘娜,周宝利,李轶修.茄子/番茄嫁接植株根系分泌物对茄子黄萎病菌的化感作用.园艺学报,2008(9)：1297-1304
27刘翔,李志文,刘莉,甜瓜枯萎病发病规律分析与防治措施.植物保护,2010(12)：30-31
28马国斌,林德佩,王叶筠.西瓜枯萎病菌镰刀菌酸对西瓜苗作用机制的初步探讨.植物病理学报,2000,30(4)：373-374
29莫贱友,郭堂勋,李华.广西瓜类作物主要病害及防治措施.长江蔬菜,2005(9)：32-33
30莫云彬,冯春梅,陈海平,董国.抗枯萎病西瓜嫁接砧木的筛选及应用.浙江农业科学,2005(3)：216-217
31戚佩坤.瓜类枯萎病菌专化型研究简介.华南农业大学学报,1995,16(4)：110-114
32苏胜举,于春雷,程洪森.西瓜不同嫁接方法对比试验.北方果树,2005,5(17)：18-19
33孙永.黄瓜嫁接后发生枯萎病的原因及预防措施.现代园艺,2010(7)：40-41
34田仁鹏,康俊根,耿丽华,颉建明,简元才,丁云花.甘蓝枯萎病抗性鉴定方法研究.中国农学通报2009,25(4)：39-42
35佟二健,符建平,隗公臣,王亚甡,杨玉娟.黄瓜嫁接砧木和嫁接方式初步筛选试验.蔬菜,2010,(4)：38-40
36翁祖信.黄瓜枯萎病抗病性鉴定方法研究-胚根接种法.中国蔬菜,1985(2)：30-34
37王登明,张学军,冯炯鑫,王叶筠,伊鸿平.甜瓜枯萎病接种方法比较试验初报.中国瓜菜,2009(5)：38-39
38王杰,张大伟,方琴.无子嫁接西瓜抗病毒病的生理生化机制研究.安徽农业大学学报,2002,29(4)：336-339
39王建明,张作刚,郭春绒,贺运春.枯萎病菌对西瓜不同抗感品种丙二醛含量及某些保护酶活性的影响.植物病理学报,2001,31(2)：152-156
40王茹华,周宝利,张启发.茄子/番茄嫁接抗病增产效果初报.中国蔬菜,2003(4)：10-11
41王素.菜豆枯萎病苗期接种方法和抗病性鉴定.中国蔬菜,1994(3)：13-15
42王守正.瓜类作物诱导抗病机制的研究.河南农业科学,2001,(3)：20-23
43王喜庆.嫁接甜瓜防病增产效果初步研究.中国西瓜甜瓜,2002(2)：22-23
44王艳飞,庞金安,马德华,李怀智.黄瓜嫁接栽培研究进展.北方园艺,2002,(1)：35-37
45魏岳荣,黄秉智,杨护,邱继水,许林兵,张明涛.香蕉感染枯萎病菌4号小种后的生理变化.园艺学进展,2008,211-215
46雷鸣.嫁接对西瓜枯萎病抗性的影响.安徽农业科学,2001,29(5)：65-656
47齐红岩,邱丽妍,滕录华,周春旭.中国农学通报,2010,26(2)：176-180
48吴波.不同砧木嫁接对甜瓜生理生化特性及枯萎病抗性影响的研究.沈阳：沈阳农业大学博士学位论文,2006
49吴凤芝,孟立君,文景芝.黄瓜根系分泌物对枯萎病病菌生长的影响.中国蔬菜,2002(5)：26-27
50吴学宏,郝京京,王红梅,高仁君.西瓜枯萎病菌几种接种方法比较试验.长江蔬菜,2003(3)：38-39
51吴宇芬,赵依杰.不同南瓜砧木对嫁接薄皮甜瓜生理的影响.中国农学通报,2007,23(11)：253-256
52谢大森,何晓明,何素娟.冬瓜、节瓜枯萎病菌人工接种技术研究.广西农业生物科学,2003,22(2)：92-95
53邢国民,亢秀平,姬青云,李丽君,祁素萍.茄果类蔬菜嫁接栽培研究进展.沈阳农业大学学报：2000,31(1)：144-146
54许勇.枯萎病菌诱导的结构性和相关酶活性的变化与西瓜枯萎病抗性的关系.果树科学,2000,17(2)：123-127
55徐丽明,篠原温,张铁中.针式嫁接法与靠接法的比较试验.中国蔬菜,2003(1)：38-39
56徐胜利,李新民,陈小青,段黄金.厚皮甜瓜嫁接育苗栽培的防病增产效果.中国蔬菜,2000(4)：16-18
57徐胜利,陈小青,陈青云.嫁接西瓜植株的生理特性及其抗枯萎病能力.中国农学通报,2004,20(2)：149-150
58徐胜利,陈小青,赵书珍,刘沙莎.哈密瓜嫁接栽培的防病增产效果研究.中 国农学通报,2004,20(1)：185-187
59薛应龙,欧阳光察,澳绍根.植物苯丙氨酸解氨酶的研究--水稻幼苗中PAL活性的动态变化.植物生理学报,1983,9(3)：301-305
60于贤昌,王立江.蔬菜嫁接的研究与应用.山东农业大学学报,1998,29(2)：249-256
61于贤昌,邢禹贤,马红,魏珉,李滨.低温胁迫下黄瓜嫁接苗和自根苗内源激素的变化.园艺学报,1999,26(6)：406-407
62云兴福,崔世茂,霍秀文.黄瓜组织中几种酶活性与其对霜霉病抗性的关系.华北农学报,1995,10(1)：92-98
63张继梅,葛志东.嫁接技术在薄皮甜瓜上的应用效果.中国西瓜甜瓜,2002(1)：26-27
64张淑梅,赵晓宇,张先成,孟利强,李晶,张云湖,王玉霞.3种瓜类枯萎病菌Fusarium oxysporum的分子检测.植物病理学报,2010,40(6)：636-641
65张玉艳,杨俊环.甜瓜枯萎病的发生与防治.吉林蔬菜,2007(6)：62
66赵淑梅,肖正璐,王海峰.大棚西瓜嫁接栽培试验研究初报.中国瓜菜,2009,(5)：40-42
67赵淑梅,肖正璐,沈彦刚.薄皮甜瓜大棚嫁接栽培试验.中国瓜菜,2010,23(4)：27-30
68周宝利,姚婷,张健,叶雪凌.苦参黄酮对黄瓜枯萎病及抗性生理的影响.华北农学报,2009,24(6)：78-82
69周红梅,毛爱军,张丽蓉,张峰,王永健,杨文才.黄瓜枯萎病接种方法及抗性遗传的研究.华北农学报,2010,25(4)：186-190
70周晓慧,Joseph N. Wolukau,李英,陈劲枫.甜瓜蔓枯病抗性与SOD、CAT和POD活性变化的关系.中国瓜菜2007(2)：4-6
71周婧,高增贵,何秀玲,庄敬华,张小飞,吴海云.甜瓜枯萎病拮抗内生细菌筛选研究.北方园艺,2008(2)：225-22
72周艳虹,喻景权,钱琼秋,黄黎锋.低温弱光对黄瓜幼苗生长及抗氧化酶活性的影响.应用生态学报,2003,14：921-924
73邹芳斌,司龙亭,李新,王莉莉.黄瓜枯萎病抗性与防御系统几种酶活性关系的研究.华北农学报.2008,23(3)：181-184
74邹庆道,张子君,吕书文,许文奎,李海涛.嫁接番茄栽培研究进展.辽宁农业科学,2002(6)：28-29
75庄炳昌,王玉民,谢雪菊,廖林,刘玉芝,徐豹.抗性不同大豆品种感染灰斑病后若干生化反应.作物学报,1993,19(6)：567-570
76郑阳霞,李能芳,钟宇.嫁接茄子苗期黄萎病抗性生理指标的研究.上海交通大学学报(农业科学版),2005,23(1)：89-91
77朱立新,李光晨.园艺通论.中国农业大学出版社,2009
78朱进,别之龙,韩晓燕,许传波,邹方益.厚皮甜瓜断根插接育苗技术.长江蔬菜,2006(8)：25-26
79朱进,别之龙,黄远,韩晓燕.不同嫁接方法对黄瓜嫁接工效和嫁接苗生长的影响.中国蔬菜,2006(9)：24-25
80朱进,别之龙,徐容,汤谧,裴芸.不同砧木嫁接对黄瓜生长、产量和品质的影响.华中农业大学学报.2006,25(6),668-671
81朱进,别之龙.嫁接对盐胁迫下黄瓜苗离子含量的影响.湖北农业科学,2010,49(7)：1642-1646
82 Abbasi A R, Hajirezaei M, Hofius D, Sonnewald U, Voll L M. Specific roles of α-and y-tocopherol in abiotic stress responses of transgenic tobacco. Plant Physiol, 2007,143:1720-1738
83 Ali M B, Eunjoo H, Yoeup P K. CO2-induced total phenolics in suspension cultures of Panax ginseng C A Mayer roots:role of antioxidants and enzymes. Plant Physiol, 2005,43:449-457
84 Alpaslan M and Gunes A. Interactive effects of boron and salinity stress on the growth, membrane permeability and mineral composition of tomato and cucumber plants. Plant Soil,2001,236,123-128
85 Bora T, Ozaktan H, Gore E, Aslan E. Biological Control of Fusarium oxysporum f. sp. melonis by Wettable Powder Formulations of the two Strains of Pseudomonas putida. J. Phytopathology,2004,152:471-475
86 Davis A R, Perkins-Veazie P, Sakata Y, Lopez-Galarza S, Maroto J V, Lee S G, Huh Y C, Sun Z Y, Miguel A, King S R, Cohen R, Lee J M. Cucurbit grafting. Crit Rev Plant Sci,2008,27(1):50-74
87 Daub M E. Peroxidation of tobacco membrane lipids by the photosensitizing toxin, Cercosporin. Plant Physiol,1982,69:1361-1364
88 Ficcadenti N, Sestili S, Annibali S, Campanelli G, Belisario A, Maccaroni M, Corazza L. Resistance to Fusarium oxysporum f. sp. melonis race 1,2 in muskmelon lines Nad-1 and Nad-2. Plant Dis,2002,86 (8):8972900
89 Joobeur T, King J J, Nolin S J. The Fusarium wilt resistance locus Form-2 of melon contains a single resistance gene with complex features. Plant J,2004,39 (3) 283-297
90 Lee J M. Cultivation of grafted vegetables. I. Current status, grafting methods, and benefits. Hortscience,1994,29(4):235-239
91 Luo M Z, Wang Y H, Frisch D, Joobeur T, Wing R A, Dean R A. Mel on bacterial artificial chromosome (BAC) library construction using improved methods and identification of clones linked to the locus conferring resistance to melon Fusarium wilt (Form-2). Genome,2001,44 (2):154-162
92 Matsuzoe N. Resistance of tomato grafted on solanum rootstock to bacterial wilt. Jpn, So Hort Sei,1991(2):176-17
93 Martyn R D, Gordon T R. Fusarium wilt of melon in compendium of cucurbit diseases. APS Press,1996,14-15
94 Meloni D A, Oliva M A, Martinez C A, Cambraia J. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot,2003,49:69-76
95 Michelet B, Boutry M. The plasma membrane H+-ATPase, a highly regulated enzyme with multiple physiological functions. Plant Physiol,1995(108):1-6
96 Ryssov-Nielson H. Measurement of the inhibition of respiration in activated sludge by a modified determination of the TTC-dehydrogenase activity. Water Res,1975, 9(12):1179-1185
97 Slinkard K, Singleton V L. Total phenol analyses:automation and comparison with manual methods. Am J Enol Viticult,1997,28:49-55
98 Sudhakar C, Lakshmi A, Giridarakumar S. Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus Alba L) under NaCl salinity. Plant Sci,2001,161:613-619
99 Trevors J T. Dehydrogenase activity in soil:A comparison between the INT and TTC assay. Soil Biol Biochem,1984,16(6):673-674
100 Wang Y H, Thomas C E, Dean R A. Genetic mapping of a Fusarium wilt resistance gene (Form-2) in melon (Cucumis melo L). Mol Breeding,2000,6:379-389
101 Wechter W P, Whitehead M P, Thomas C E. Identification of a randomly amplified polymorphic DNA marker linked to the Fom-2 Fusarium wilt resistance gene in muskmelon MR-1. Mol Plant Pathol,1995,85 (10):1245-1249
102 Wechter W P, Dean R A. Development of sequence specific primers that amplified a 115kb DNA marker for race 1 Fusarium wilt resistance in Cucumis melo L. Hort science,1998,33 (2):219-292
103 Vurro M, Ellis B E. Effect of fungal toxin on induction of phenylalanine ammonia-lyase in elicited cultures of hybrid poplar. Plant Sci,1997,126:29-38
104 Yamamoto Y, Kobayashi Y, Matumoto H. Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in Pea Roots. Plant Physiol,2001,125:199-208
2 别之龙,朱进,黄远.黄瓜断根嫁接工厂化穴盘育苗技术.中国蔬菜,2006(8)：48-49
3 蔡竹固,陈瑞祥,童伯开.台湾甜瓜蔓割病菌之生理小种.植保会刊,1999,41：139-143
4 陈士海,季海军.嫁接黄瓜枯萎病的发生与防治.植物保护,2010(9),23
5 崔健,宋云云,刘素芹,张淑霞.厚皮甜瓜舌形靠接育苗技术.西北园艺,2008(7)：21-22
6 韩金星,洪日新,周林,何毅,黄金艳,覃斯华,李文信,樊学军,李天艳.西瓜、黄瓜、甜瓜等瓜类枯萎病研究进展.中国瓜菜,2009(2)：32-35
7 吉加兵.西瓜枯萎病苗期抗性鉴定方法的探讨.中国西瓜甜瓜,1992(1)：35-39
8 李金玉,颜雪,黄琼,赵惠新,李冠.甜瓜抗枯萎病基因同源序列克隆与序列分析.生物技术,2006,16(3)：3-9
9 高梅秀,李树和,刘玉芹等.不同砧木对茄子抗病性、生理活性及产量的影响.园艺学报,2001,28(5)：463-465
10顾卫红,宋荣浩.西瓜种质资源主要园艺学性状的评价.上海农学院学报,1996,14(4)：294-299
1 1顾卫红,王鸣.甜瓜种质资源对枯萎病抗性的苗期人工接种鉴定.中国西瓜甜瓜,1989(1)： 1-8
12顾卫红,王鸣.几种氧化酶同工酶与甜瓜抗枯萎病关系研究.北方园艺.1992(2)：7-9
13黄奔立,许云东,张顺琦,陈学好.根系分泌物影响黄瓜枯萎病抗性的机理研究.扬州大学学报,2007,28(3)：77-81
14黄志光,乐秋歆,吴建能.嫁接与非嫁接西瓜抗枯萎病田间试验简报.上海农业科技,2010(2)：91
15蒋有条.西瓜甜瓜的嫁接栽培(综述)中国西瓜甜瓜,1989(1)：27-31
16雷鸣.嫁接对西瓜枯萎病抗性的影响.安徽农业科学,2001,29(5)：655-656
17李光,李淑菊,王惠哲.兼抗黄瓜枯萎病、角斑病和黑星病育种材料苗期筛选.天津农业科学,2007,13(3)：23-25
18李合生.植物生理生化实验原理和技术.北京：高等教育出版社,2000,205-278
19李合生.现代植物生理学.北京：高等教育出版社,2002：505
20李振卓,祁佐宽,邓守哲.不同嫁接方法对薄皮甜瓜生长速度成活率及抗冷性作用研究.农业与技术,2009,29(4)：61-65
21李明远,李兴红,黄金宝,严红,张涛涛,周莹.瓜类枯萎病及其防治.中国蔬菜,2008(9)：57-60
22刘春艳,王万立,郝永娟,王勇.大棚甜瓜枯萎病的发生及综合防治.农业科技通,2010(1)：171-172
23刘文革.甜瓜(Cucumis melo)基因目录.中国西瓜甜瓜,2000(2)：37-40
24刘文革.甜瓜基因目录(2002).中国西瓜甜瓜,2004(4)：44-45
25刘国生.微生物学实验技术.北京：科学出版社,2007
26刘娜,周宝利,李轶修.茄子/番茄嫁接植株根系分泌物对茄子黄萎病菌的化感作用.园艺学报,2008(9)：1297-1304
27刘翔,李志文,刘莉,甜瓜枯萎病发病规律分析与防治措施.植物保护,2010(12)：30-31
28马国斌,林德佩,王叶筠.西瓜枯萎病菌镰刀菌酸对西瓜苗作用机制的初步探讨.植物病理学报,2000,30(4)：373-374
29莫贱友,郭堂勋,李华.广西瓜类作物主要病害及防治措施.长江蔬菜,2005(9)：32-33
30莫云彬,冯春梅,陈海平,董国.抗枯萎病西瓜嫁接砧木的筛选及应用.浙江农业科学,2005(3)：216-217
31戚佩坤.瓜类枯萎病菌专化型研究简介.华南农业大学学报,1995,16(4)：110-114
32苏胜举,于春雷,程洪森.西瓜不同嫁接方法对比试验.北方果树,2005,5(17)：18-19
33孙永.黄瓜嫁接后发生枯萎病的原因及预防措施.现代园艺,2010(7)：40-41
34田仁鹏,康俊根,耿丽华,颉建明,简元才,丁云花.甘蓝枯萎病抗性鉴定方法研究.中国农学通报2009,25(4)：39-42
35佟二健,符建平,隗公臣,王亚甡,杨玉娟.黄瓜嫁接砧木和嫁接方式初步筛选试验.蔬菜,2010,(4)：38-40
36翁祖信.黄瓜枯萎病抗病性鉴定方法研究-胚根接种法.中国蔬菜,1985(2)：30-34
37王登明,张学军,冯炯鑫,王叶筠,伊鸿平.甜瓜枯萎病接种方法比较试验初报.中国瓜菜,2009(5)：38-39
38王杰,张大伟,方琴.无子嫁接西瓜抗病毒病的生理生化机制研究.安徽农业大学学报,2002,29(4)：336-339
39王建明,张作刚,郭春绒,贺运春.枯萎病菌对西瓜不同抗感品种丙二醛含量及某些保护酶活性的影响.植物病理学报,2001,31(2)：152-156
40王茹华,周宝利,张启发.茄子/番茄嫁接抗病增产效果初报.中国蔬菜,2003(4)：10-11
41王素.菜豆枯萎病苗期接种方法和抗病性鉴定.中国蔬菜,1994(3)：13-15
42王守正.瓜类作物诱导抗病机制的研究.河南农业科学,2001,(3)：20-23
43王喜庆.嫁接甜瓜防病增产效果初步研究.中国西瓜甜瓜,2002(2)：22-23
44王艳飞,庞金安,马德华,李怀智.黄瓜嫁接栽培研究进展.北方园艺,2002,(1)：35-37
45魏岳荣,黄秉智,杨护,邱继水,许林兵,张明涛.香蕉感染枯萎病菌4号小种后的生理变化.园艺学进展,2008,211-215
46雷鸣.嫁接对西瓜枯萎病抗性的影响.安徽农业科学,2001,29(5)：65-656
47齐红岩,邱丽妍,滕录华,周春旭.中国农学通报,2010,26(2)：176-180
48吴波.不同砧木嫁接对甜瓜生理生化特性及枯萎病抗性影响的研究.沈阳：沈阳农业大学博士学位论文,2006
49吴凤芝,孟立君,文景芝.黄瓜根系分泌物对枯萎病病菌生长的影响.中国蔬菜,2002(5)：26-27
50吴学宏,郝京京,王红梅,高仁君.西瓜枯萎病菌几种接种方法比较试验.长江蔬菜,2003(3)：38-39
51吴宇芬,赵依杰.不同南瓜砧木对嫁接薄皮甜瓜生理的影响.中国农学通报,2007,23(11)：253-256
52谢大森,何晓明,何素娟.冬瓜、节瓜枯萎病菌人工接种技术研究.广西农业生物科学,2003,22(2)：92-95
53邢国民,亢秀平,姬青云,李丽君,祁素萍.茄果类蔬菜嫁接栽培研究进展.沈阳农业大学学报：2000,31(1)：144-146
54许勇.枯萎病菌诱导的结构性和相关酶活性的变化与西瓜枯萎病抗性的关系.果树科学,2000,17(2)：123-127
55徐丽明,篠原温,张铁中.针式嫁接法与靠接法的比较试验.中国蔬菜,2003(1)：38-39
56徐胜利,李新民,陈小青,段黄金.厚皮甜瓜嫁接育苗栽培的防病增产效果.中国蔬菜,2000(4)：16-18
57徐胜利,陈小青,陈青云.嫁接西瓜植株的生理特性及其抗枯萎病能力.中国农学通报,2004,20(2)：149-150
58徐胜利,陈小青,赵书珍,刘沙莎.哈密瓜嫁接栽培的防病增产效果研究.中 国农学通报,2004,20(1)：185-187
59薛应龙,欧阳光察,澳绍根.植物苯丙氨酸解氨酶的研究--水稻幼苗中PAL活性的动态变化.植物生理学报,1983,9(3)：301-305
60于贤昌,王立江.蔬菜嫁接的研究与应用.山东农业大学学报,1998,29(2)：249-256
61于贤昌,邢禹贤,马红,魏珉,李滨.低温胁迫下黄瓜嫁接苗和自根苗内源激素的变化.园艺学报,1999,26(6)：406-407
62云兴福,崔世茂,霍秀文.黄瓜组织中几种酶活性与其对霜霉病抗性的关系.华北农学报,1995,10(1)：92-98
63张继梅,葛志东.嫁接技术在薄皮甜瓜上的应用效果.中国西瓜甜瓜,2002(1)：26-27
64张淑梅,赵晓宇,张先成,孟利强,李晶,张云湖,王玉霞.3种瓜类枯萎病菌Fusarium oxysporum的分子检测.植物病理学报,2010,40(6)：636-641
65张玉艳,杨俊环.甜瓜枯萎病的发生与防治.吉林蔬菜,2007(6)：62
66赵淑梅,肖正璐,王海峰.大棚西瓜嫁接栽培试验研究初报.中国瓜菜,2009,(5)：40-42
67赵淑梅,肖正璐,沈彦刚.薄皮甜瓜大棚嫁接栽培试验.中国瓜菜,2010,23(4)：27-30
68周宝利,姚婷,张健,叶雪凌.苦参黄酮对黄瓜枯萎病及抗性生理的影响.华北农学报,2009,24(6)：78-82
69周红梅,毛爱军,张丽蓉,张峰,王永健,杨文才.黄瓜枯萎病接种方法及抗性遗传的研究.华北农学报,2010,25(4)：186-190
70周晓慧,Joseph N. Wolukau,李英,陈劲枫.甜瓜蔓枯病抗性与SOD、CAT和POD活性变化的关系.中国瓜菜2007(2)：4-6
71周婧,高增贵,何秀玲,庄敬华,张小飞,吴海云.甜瓜枯萎病拮抗内生细菌筛选研究.北方园艺,2008(2)：225-22
72周艳虹,喻景权,钱琼秋,黄黎锋.低温弱光对黄瓜幼苗生长及抗氧化酶活性的影响.应用生态学报,2003,14：921-924
73邹芳斌,司龙亭,李新,王莉莉.黄瓜枯萎病抗性与防御系统几种酶活性关系的研究.华北农学报.2008,23(3)：181-184
74邹庆道,张子君,吕书文,许文奎,李海涛.嫁接番茄栽培研究进展.辽宁农业科学,2002(6)：28-29
75庄炳昌,王玉民,谢雪菊,廖林,刘玉芝,徐豹.抗性不同大豆品种感染灰斑病后若干生化反应.作物学报,1993,19(6)：567-570
76郑阳霞,李能芳,钟宇.嫁接茄子苗期黄萎病抗性生理指标的研究.上海交通大学学报(农业科学版),2005,23(1)：89-91
77朱立新,李光晨.园艺通论.中国农业大学出版社,2009
78朱进,别之龙,韩晓燕,许传波,邹方益.厚皮甜瓜断根插接育苗技术.长江蔬菜,2006(8)：25-26
79朱进,别之龙,黄远,韩晓燕.不同嫁接方法对黄瓜嫁接工效和嫁接苗生长的影响.中国蔬菜,2006(9)：24-25
80朱进,别之龙,徐容,汤谧,裴芸.不同砧木嫁接对黄瓜生长、产量和品质的影响.华中农业大学学报.2006,25(6),668-671
81朱进,别之龙.嫁接对盐胁迫下黄瓜苗离子含量的影响.湖北农业科学,2010,49(7)：1642-1646
82 Abbasi A R, Hajirezaei M, Hofius D, Sonnewald U, Voll L M. Specific roles of α-and y-tocopherol in abiotic stress responses of transgenic tobacco. Plant Physiol, 2007,143:1720-1738
83 Ali M B, Eunjoo H, Yoeup P K. CO2-induced total phenolics in suspension cultures of Panax ginseng C A Mayer roots:role of antioxidants and enzymes. Plant Physiol, 2005,43:449-457
84 Alpaslan M and Gunes A. Interactive effects of boron and salinity stress on the growth, membrane permeability and mineral composition of tomato and cucumber plants. Plant Soil,2001,236,123-128
85 Bora T, Ozaktan H, Gore E, Aslan E. Biological Control of Fusarium oxysporum f. sp. melonis by Wettable Powder Formulations of the two Strains of Pseudomonas putida. J. Phytopathology,2004,152:471-475
86 Davis A R, Perkins-Veazie P, Sakata Y, Lopez-Galarza S, Maroto J V, Lee S G, Huh Y C, Sun Z Y, Miguel A, King S R, Cohen R, Lee J M. Cucurbit grafting. Crit Rev Plant Sci,2008,27(1):50-74
87 Daub M E. Peroxidation of tobacco membrane lipids by the photosensitizing toxin, Cercosporin. Plant Physiol,1982,69:1361-1364
88 Ficcadenti N, Sestili S, Annibali S, Campanelli G, Belisario A, Maccaroni M, Corazza L. Resistance to Fusarium oxysporum f. sp. melonis race 1,2 in muskmelon lines Nad-1 and Nad-2. Plant Dis,2002,86 (8):8972900
89 Joobeur T, King J J, Nolin S J. The Fusarium wilt resistance locus Form-2 of melon contains a single resistance gene with complex features. Plant J,2004,39 (3) 283-297
90 Lee J M. Cultivation of grafted vegetables. I. Current status, grafting methods, and benefits. Hortscience,1994,29(4):235-239
91 Luo M Z, Wang Y H, Frisch D, Joobeur T, Wing R A, Dean R A. Mel on bacterial artificial chromosome (BAC) library construction using improved methods and identification of clones linked to the locus conferring resistance to melon Fusarium wilt (Form-2). Genome,2001,44 (2):154-162
92 Matsuzoe N. Resistance of tomato grafted on solanum rootstock to bacterial wilt. Jpn, So Hort Sei,1991(2):176-17
93 Martyn R D, Gordon T R. Fusarium wilt of melon in compendium of cucurbit diseases. APS Press,1996,14-15
94 Meloni D A, Oliva M A, Martinez C A, Cambraia J. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot,2003,49:69-76
95 Michelet B, Boutry M. The plasma membrane H+-ATPase, a highly regulated enzyme with multiple physiological functions. Plant Physiol,1995(108):1-6
96 Ryssov-Nielson H. Measurement of the inhibition of respiration in activated sludge by a modified determination of the TTC-dehydrogenase activity. Water Res,1975, 9(12):1179-1185
97 Slinkard K, Singleton V L. Total phenol analyses:automation and comparison with manual methods. Am J Enol Viticult,1997,28:49-55
98 Sudhakar C, Lakshmi A, Giridarakumar S. Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus Alba L) under NaCl salinity. Plant Sci,2001,161:613-619
99 Trevors J T. Dehydrogenase activity in soil:A comparison between the INT and TTC assay. Soil Biol Biochem,1984,16(6):673-674
100 Wang Y H, Thomas C E, Dean R A. Genetic mapping of a Fusarium wilt resistance gene (Form-2) in melon (Cucumis melo L). Mol Breeding,2000,6:379-389
101 Wechter W P, Whitehead M P, Thomas C E. Identification of a randomly amplified polymorphic DNA marker linked to the Fom-2 Fusarium wilt resistance gene in muskmelon MR-1. Mol Plant Pathol,1995,85 (10):1245-1249
102 Wechter W P, Dean R A. Development of sequence specific primers that amplified a 115kb DNA marker for race 1 Fusarium wilt resistance in Cucumis melo L. Hort science,1998,33 (2):219-292
103 Vurro M, Ellis B E. Effect of fungal toxin on induction of phenylalanine ammonia-lyase in elicited cultures of hybrid poplar. Plant Sci,1997,126:29-38
104 Yamamoto Y, Kobayashi Y, Matumoto H. Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in Pea Roots. Plant Physiol,2001,125:199-208