蜜炼鲜人参补液化学成分及其生物活性研究
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摘要
蜜炼鲜人参补液是以鲜人参、大豆异黄酮、宁夏枸杞子、灵芝、鹿茸、林蛙油、广西蛤蚧以及鹿胎膏等动物植物提取物为主要原料,经长白山特产天然椴树蜜长时间蜜炼精制而成。蜜炼诸种名贵中药的宗旨是通过利用椴树蜜既溶解水溶性成分又能溶解脂溶性成分的特点,精炼每味药性和促进相互协同作用,将具有不同生物功能的各类成分熔融一起发挥功效,以调节机体免疫功能、激发机体的内在活力,最终达到增强中老年及亚健康人群机体免疫力、延缓衰老、提高生活质量的目的。
本文主要做了以下几方面工作:
1.综述了蜜炼鲜人参补液主要名贵药材的研究概况。
2.首次从吉林省靖宇县通宝中药材科技开发有限公司生产的蜜炼鲜人参补液产品中分离出8个化合物,通过理化性质和波谱解析,鉴定了它们的结构。
3.首次对蜜炼鲜人参补液的多糖成分进行研究,应用经典的水提醇沉法提取蜜炼鲜人参补液的活性多糖成分,经Sevage法与木瓜蛋白酶法相结合去除游离蛋白,H2O2氧化法去除色素,透析法去除小分子无机盐,葡聚糖凝胶色谱法初步分离纯化得到6个组成均一的多糖成分,并进行了单糖组成和分子量的测定。
4.首次对蜜炼鲜人参补液中提取的粗多糖成分进行了抗衰老活性研究。通过D-半乳糖法致衰老模型小鼠,同时给予蜜炼鲜人参补液粗多糖12.5,25,50mg·kg-1·d-1连续灌胃6周,观察衰老模型小鼠系统功能的影响,并对反映机体衰老的四种生化指标-超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)和一氧化氮合酶(NOS)的活力进行测定。实验结果表明,蜜炼鲜人参补液粗多糖能使D-半乳糖法致衰老模型小鼠血浆和肝组织中MDA明显下降,SOD活力明显上升,GSH-PX的活力明显上升。说明蜜炼鲜人参补液中提取的粗多糖有抗衰老作用,其机制可能与清除自由基有关。
In the thesis,the chemical constituents and bioactivities about the liquid supplements of fresh panax Ginseng refined with honey were studied for the first time. The liquid supplements contain eight valuable Chinese herbals,such as fresh Panax ginseng C.A.Meyer,Soybean isoflavone,Lycium barbarum L.,Ganoderma lucidum et al. There are many activive compounds,including steroid hormone,Saponins,alkaloids,flavonoids,polysaccharide,protein and inorganic elements,which have many hygienical functions.
The polysaccharide constituent of the liquid supplements of fresh panax Ginseng refined with honey was studied for the first time. Six kind of polysaccharides were separated and purified by the method of water extraction and alcohol deposit, then the protein was removed by the method of Sevage and papain hydrolysis. Last,the pigment was eliminated by H2O2 oxidation, micromolecule and mineral salt were taken off by gel chromatography. The six polysaccharides were named as GPS-I、GPS-II、GPS-III、GPS-IV、GPS-V、GPS-VI whose molecular weight is 56302,30372,10601,10364,9247,4667seperately.
On the other hand ,the other chemical constituents of the liquid supplements of fresh panax Ginseng refined with honey were investigated through the separation and purification by column chromatography of silica gel,eversed phase silica column, semi-preparative high performance liquid chromatography and recrystallization methods. 7 kinds of compounds were identified according to their physical-chemical properties and spectrum analysis including Sucrose(1),β-D-glucopyranosyl B glycoside(2), 4,2,4-trihydroxy chalcone(3), 7, 4-dihydroxy- flavanone-7-O-β-D-glucoside(4),4,2',4'-trihydroxy chalcone-4-O-β-glucopyranoside(5), pheny- lacetic acid(6), 20 (S) - ginsenoside Rh2(7), 20 (S) - ginsenoside Rh1(8).
The total polysaccharide separated from the liquid supplements of fresh panax ginseng refined with honey is studied for the first time on anti-aging the classical D-galactose-induced mice aging model experiment,the animals were administrated with the total polysaccharide for 6 weeks in high,moderate and low three doses respectively,and the four biochemical parameters of aging were measured.
The results showed that continuous subcutaneous injection of mice 6 weeks to D-galactose, measured in model group mice in the liver and plasma SOD decreased, MDA value increased; renal tissue GSH-PX dynamism and vitality of NOS have declined.
Compared with normal group, model group mice aging of plasma superoxide dismutase significantly reduced viability (P<0.05), MDA content increased significantly (P<0.01). Superoxide dismutase indirectly reduce the dynamic response of the body's ability to clear the drop in oxygen free radicals, and the MDA content increased, indicating that the body to deepen the level of lipid peroxidation, which combination of the two biochemical markers, reflecting the emergence of the body the signs of aging on this experiment with D-galactose-made model of successful aging.
Compared with model group, the polysaccharide of the liquid supplements of fresh panax ginseng L. refined with Honey (GSPS)25mg ? kg-1 ? d-1, 50mg ? kg-1 ? d-1 dose group significantly increased superoxide dismutase vitality (P <0.05) to enhance the body of oxygen free radicals on the inhibition rate was significantly reduced in liver tissue and plasma MDA levels (P<0.05). Lian fresh ginseng honey Polysaccharide rehydration 50mg ? kg-1 ? d-1 group significantly increased superoxide dismutase glutathione peroxidase vitality (P <0.05). Lian fresh ginseng and honey Polysaccharide rehydration high, medium and low dose group of the three NOS activity are not clear. Description of fresh ginseng honey Lian Polysaccharide rehydration has a good anti-aging effect, which may be related with the removal of free radicals. Pharmacological study shows that total polysaccharide can enhance and strengthen the body’s ability of anti-senescing.
本文主要做了以下几方面工作:
1.综述了蜜炼鲜人参补液主要名贵药材的研究概况。
2.首次从吉林省靖宇县通宝中药材科技开发有限公司生产的蜜炼鲜人参补液产品中分离出8个化合物,通过理化性质和波谱解析,鉴定了它们的结构。
3.首次对蜜炼鲜人参补液的多糖成分进行研究,应用经典的水提醇沉法提取蜜炼鲜人参补液的活性多糖成分,经Sevage法与木瓜蛋白酶法相结合去除游离蛋白,H2O2氧化法去除色素,透析法去除小分子无机盐,葡聚糖凝胶色谱法初步分离纯化得到6个组成均一的多糖成分,并进行了单糖组成和分子量的测定。
4.首次对蜜炼鲜人参补液中提取的粗多糖成分进行了抗衰老活性研究。通过D-半乳糖法致衰老模型小鼠,同时给予蜜炼鲜人参补液粗多糖12.5,25,50mg·kg-1·d-1连续灌胃6周,观察衰老模型小鼠系统功能的影响,并对反映机体衰老的四种生化指标-超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)和一氧化氮合酶(NOS)的活力进行测定。实验结果表明,蜜炼鲜人参补液粗多糖能使D-半乳糖法致衰老模型小鼠血浆和肝组织中MDA明显下降,SOD活力明显上升,GSH-PX的活力明显上升。说明蜜炼鲜人参补液中提取的粗多糖有抗衰老作用,其机制可能与清除自由基有关。
In the thesis,the chemical constituents and bioactivities about the liquid supplements of fresh panax Ginseng refined with honey were studied for the first time. The liquid supplements contain eight valuable Chinese herbals,such as fresh Panax ginseng C.A.Meyer,Soybean isoflavone,Lycium barbarum L.,Ganoderma lucidum et al. There are many activive compounds,including steroid hormone,Saponins,alkaloids,flavonoids,polysaccharide,protein and inorganic elements,which have many hygienical functions.
The polysaccharide constituent of the liquid supplements of fresh panax Ginseng refined with honey was studied for the first time. Six kind of polysaccharides were separated and purified by the method of water extraction and alcohol deposit, then the protein was removed by the method of Sevage and papain hydrolysis. Last,the pigment was eliminated by H2O2 oxidation, micromolecule and mineral salt were taken off by gel chromatography. The six polysaccharides were named as GPS-I、GPS-II、GPS-III、GPS-IV、GPS-V、GPS-VI whose molecular weight is 56302,30372,10601,10364,9247,4667seperately.
On the other hand ,the other chemical constituents of the liquid supplements of fresh panax Ginseng refined with honey were investigated through the separation and purification by column chromatography of silica gel,eversed phase silica column, semi-preparative high performance liquid chromatography and recrystallization methods. 7 kinds of compounds were identified according to their physical-chemical properties and spectrum analysis including Sucrose(1),β-D-glucopyranosyl B glycoside(2), 4,2,4-trihydroxy chalcone(3), 7, 4-dihydroxy- flavanone-7-O-β-D-glucoside(4),4,2',4'-trihydroxy chalcone-4-O-β-glucopyranoside(5), pheny- lacetic acid(6), 20 (S) - ginsenoside Rh2(7), 20 (S) - ginsenoside Rh1(8).
The total polysaccharide separated from the liquid supplements of fresh panax ginseng refined with honey is studied for the first time on anti-aging the classical D-galactose-induced mice aging model experiment,the animals were administrated with the total polysaccharide for 6 weeks in high,moderate and low three doses respectively,and the four biochemical parameters of aging were measured.
The results showed that continuous subcutaneous injection of mice 6 weeks to D-galactose, measured in model group mice in the liver and plasma SOD decreased, MDA value increased; renal tissue GSH-PX dynamism and vitality of NOS have declined.
Compared with normal group, model group mice aging of plasma superoxide dismutase significantly reduced viability (P<0.05), MDA content increased significantly (P<0.01). Superoxide dismutase indirectly reduce the dynamic response of the body's ability to clear the drop in oxygen free radicals, and the MDA content increased, indicating that the body to deepen the level of lipid peroxidation, which combination of the two biochemical markers, reflecting the emergence of the body the signs of aging on this experiment with D-galactose-made model of successful aging.
Compared with model group, the polysaccharide of the liquid supplements of fresh panax ginseng L. refined with Honey (GSPS)25mg ? kg-1 ? d-1, 50mg ? kg-1 ? d-1 dose group significantly increased superoxide dismutase vitality (P <0.05) to enhance the body of oxygen free radicals on the inhibition rate was significantly reduced in liver tissue and plasma MDA levels (P<0.05). Lian fresh ginseng honey Polysaccharide rehydration 50mg ? kg-1 ? d-1 group significantly increased superoxide dismutase glutathione peroxidase vitality (P <0.05). Lian fresh ginseng and honey Polysaccharide rehydration high, medium and low dose group of the three NOS activity are not clear. Description of fresh ginseng honey Lian Polysaccharide rehydration has a good anti-aging effect, which may be related with the removal of free radicals. Pharmacological study shows that total polysaccharide can enhance and strengthen the body’s ability of anti-senescing.
引文
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