固本止咳平喘颗粒治疗哮喘的抗炎、免疫实验研究
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摘要
目的:固本止咳平喘颗粒是我师在多年临床基础上优化组合而制成的治疗哮喘的有效方剂,固本止咳平喘颗粒主治支气管哮喘(虚哮)之咳嗽,喘促,胸闷,喉鸣,气短,乏力,出汗,在临床应用中疗效突出。本课题以中医理论为指导,通过固本止咳平喘颗粒的抗炎实验和免疫实验,评价和探索固本止咳平喘颗粒治疗支气管哮喘的作用机制,为临床用药和开发新药提供依据。
方法:将4个实验所需的小鼠及大鼠按照实验要求均分成五组,即空白对照组,固本止咳平喘颗粒低、中、高剂量组,桂龙咳喘宁胶囊组。分别观察固本止咳平喘颗粒对急性炎症及慢性炎症的作用;以及从体液免疫和细胞免疫两方面观察固本止咳平喘颗粒的免疫调节作用。
结果:固本止咳平喘颗粒可抑制二甲苯所致的小鼠耳廓急性炎症肿胀;抑制大鼠琼脂肉芽慢性肿胀;增加小鼠血清溶血素抗体生成量;减弱2,4-二硝基氯苯所致小鼠迟发超敏反应。明显对抗急性炎症和慢性炎症,调节体液免疫和细胞免疫。
结论:研究结果表明,固本止咳平喘颗粒具有明显的抗炎和免疫调节作用。为该制剂临床用药和进一步开发研制新药提供了一定的药理学依据。
Purpose:In fact I cough heavily on the particles are based on optimum combination for clinical treatment of asthma made effective prescriptions, cough and foundation of bronchial asthma (virtual particles indications of cough, asthma wheezing) promoting, bosom frowsty, larynx, shortness of breath, fatigue, sweating, curative effect in clinical applications. This topic in TCM theory as the guide, cough and foundation through the anti-inflammatory experiments and particle immune experiment, evaluation and explore consoliated cost cough heavily in treating bronchial asthma with particle mechanism for clinical use and development, and provide basis for new drugs.
Material and method:First, four experiments of mice and rats required in accordance with the requirements of experiments are divided into six groups, in fact, control and cough particle high, medium and low dose group GuiLong, cough and asthma ning capsule group and dexamethasone chips group. In fact cough heavily were observed for acute inflammation and the effect of chronic inflammation, And humoral immune cells from the two aspects of observation and immune to cough heavily particles in fact immunosuppression.
Results:
In fact cough heavily particles may inhibit xylene-induced ear acute inflammatory swelling, Restrain rats AGAR granulation swollen chronic, Increases in serum hemolysin antibody cargoes, Weakened 2,4-diflooronitrohenzene nitro chlorobenzene mice late-onset hypersensitiity reactions. Obviously against acute inflammation and chronic inflammation, humoral immune cells and immune suppression.
Conclusion:
The research results show that the foundation cough heavily particle has obvious anti-inflammation and immune suppression effect of treatment of asthma, and has good effect. For the preparation of clinical medicine and further development provides a new pharmacology.
方法:将4个实验所需的小鼠及大鼠按照实验要求均分成五组,即空白对照组,固本止咳平喘颗粒低、中、高剂量组,桂龙咳喘宁胶囊组。分别观察固本止咳平喘颗粒对急性炎症及慢性炎症的作用;以及从体液免疫和细胞免疫两方面观察固本止咳平喘颗粒的免疫调节作用。
结果:固本止咳平喘颗粒可抑制二甲苯所致的小鼠耳廓急性炎症肿胀;抑制大鼠琼脂肉芽慢性肿胀;增加小鼠血清溶血素抗体生成量;减弱2,4-二硝基氯苯所致小鼠迟发超敏反应。明显对抗急性炎症和慢性炎症,调节体液免疫和细胞免疫。
结论:研究结果表明,固本止咳平喘颗粒具有明显的抗炎和免疫调节作用。为该制剂临床用药和进一步开发研制新药提供了一定的药理学依据。
Purpose:In fact I cough heavily on the particles are based on optimum combination for clinical treatment of asthma made effective prescriptions, cough and foundation of bronchial asthma (virtual particles indications of cough, asthma wheezing) promoting, bosom frowsty, larynx, shortness of breath, fatigue, sweating, curative effect in clinical applications. This topic in TCM theory as the guide, cough and foundation through the anti-inflammatory experiments and particle immune experiment, evaluation and explore consoliated cost cough heavily in treating bronchial asthma with particle mechanism for clinical use and development, and provide basis for new drugs.
Material and method:First, four experiments of mice and rats required in accordance with the requirements of experiments are divided into six groups, in fact, control and cough particle high, medium and low dose group GuiLong, cough and asthma ning capsule group and dexamethasone chips group. In fact cough heavily were observed for acute inflammation and the effect of chronic inflammation, And humoral immune cells from the two aspects of observation and immune to cough heavily particles in fact immunosuppression.
Results:
In fact cough heavily particles may inhibit xylene-induced ear acute inflammatory swelling, Restrain rats AGAR granulation swollen chronic, Increases in serum hemolysin antibody cargoes, Weakened 2,4-diflooronitrohenzene nitro chlorobenzene mice late-onset hypersensitiity reactions. Obviously against acute inflammation and chronic inflammation, humoral immune cells and immune suppression.
Conclusion:
The research results show that the foundation cough heavily particle has obvious anti-inflammation and immune suppression effect of treatment of asthma, and has good effect. For the preparation of clinical medicine and further development provides a new pharmacology.
引文
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[10]杨琛.中西医结合治疗支气管哮喘68例.河南中医.2009, 29(5): 491-491.
[11]李玉兰.补肾固本汤对小儿哮喘缓解期干预的临床观察.中国中医急症.2005,14(7):639-640.
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[13]朱慧华,陈燕萍,虞坚尔等.辛苍汤对小儿支气管哮喘缓解期慢性气道炎症的治疗作用.中西医结合学报.2005,1(3):23-27.
[14]金戈.平喘合剂治疗小儿支气管哮喘等咳喘症35例.江苏中医学院学报.2001, 18(1): 23-25.
[15]杨穗红.黎氏哮喘方治疗小儿支气管哮喘60例疗效观察.新中医.2005,3(37):30-31.
[16]季风刚.乌梅丸汤剂治疗激素依赖型哮喘26例.实用中医内科杂志.2001, 15(1): 11.
[17]高修安,朱锦善.海龙蠲哮方合咳喘平涌泉穴位贴敷治疗小儿支气管哮喘发作期临床观察.中国中西医结合杂志.2005,8(25):738-739.
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[20]刘立席,谢安树.自拟久咳方治疗小儿咳嗽变异型哮喘38例.四川中医.2009, 27(8): 104-105.
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[22]周志荣.复方太子参止咳益气散治疗支气管哮喘2 180例疗效观察.中国民族医药杂志.2005(5):12.
[23]文灿新.肃肺汤治疗小儿哮喘74例.中国中医药信息杂志.2004,11(12):1 096.
[24]刘克丽.定哮饮治疗儿童哮喘的临床研究.湖南中医学院学报.2006,26(4):40-41.
[25]杜新玲.中西医结合治疗45例支气管哮喘的临床观察.中国实用医药.2009, 4(8): 169-170.
[26]杜旭隆,杨玉虎,席武.中西医结合治疗小儿咳喘哮50例.四川中医.2009, 27(7): 91-92.
[27]李凤娥,朱海军,魁发瑞.中西医结合治疗小儿支气管哮喘46例.西北民族大学学报.2008, 29(72):77-78.
[28]李淑英.中西医结合治疗小儿支气管哮喘46例疗效观察.西北民族大学学报.2009, 13:741-741.
[29]米志宽,李锦春,符国儒.中西医结合治疗小儿支气管哮喘60例.陕西中医.2009,30(3):290-291.
[30]张光强.中西医结合治疗支气管哮喘急性发作30例.中国民间疗法.2009,17(6):37-37.
[31]侯爱凤,于豪.中西医结合治疗小儿支气管哮喘.中国民间疗法.2009,17(1):44-44.
[32]蔡茜虹,郭雪霞,朱敏.中西医结合治疗64例支气管哮喘.广东医学.2008,1(1):145-146.
[33]谢洪波,安美华,刘建秋.中西医结合治疗支气管哮喘46例.吉林中医药.2006,26(1):41.
[34]赵杰,金沈蓉,王琰.中西医结合治疗支气管哮喘36例临床观察.四川中医.2007,25(5):39.
[35]毕美芬.药物穴位敷贴治疗小儿支气管哮喘160例.实用中西医结合临床.2005, 5(5): 54-55.
[36]刘静仪,林如平.冬病夏治法治疗支气管哮喘76例疗效观察.成都医药.2004, 30(5): 462-462.
[37]齐玲,胥丹桂.三伏天中药穴位敷贴治疗缓解期哮喘患儿疗效观察及护理.护理学杂志.2009,24(11):40-41.
[38]陈颂贤.穴位贴药治疗小儿支气管哮喘38例.Joumal of Extemal Therapy of TCM. 2009.18(3):36-36.
[39]王晓贤.针灸孔最穴治疗支气管哮喘.中国针灸.2009, 29(4): 326-326.
[40]王正礼,朱月伟.针刺后擦背合贴敷治疗支气管哮喘130例.浙江中医杂志.2009,44(4) :288.
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