可重复使用的骨生长定量检测盒的研制
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摘要
目的：设计并制作可重复使用的骨生长定量检测盒(bone harvest chamber,BHC),验证其是否可置入贵州小型猪骨骼中并在同一动物体内重复使用。方法：(1)根据贵州小型猪肱骨和股骨的解剖资料设计并制作BHC。(2)贵州小型猪两只,将已填装好成骨材料的BHC分别植入其肱骨和股骨干骺端。术后两周行影像学检查,术后4周更换新填装好成骨材料的生长盒。结果：1、BHC尺寸大小合适2、BHC可置入小型猪体内,固定牢固,并可在同一动物体内重复使用。结论：BHC可置入小型猪肱骨和股骨干骺端,可装入不同的成骨材料,组织相容性好,并可重复使用,操作简单易行。
Objective:(1)We create the bone harvest chamber(BHC), then verify the possibility that if it can be imbedded into the bones of the Guizhou minipig and used repeatedly in the same minipig. Methods:(1) We designed and made the BHC according to the anatomical data of the humerus and femur of GuiZhou minipigs. (2)Two Guizhou minipig were used. The BHC which were loaded the bone graft materials were imbedded into humeral and femoral metaphysis of the Guizhou minipigs. Checked them by imaging detection at postoperative 2 weeks,and harvested chambers at postoperative 4 weeks while imbeded new chambers. Results:1、The dimension of the BHC is suitable.2、BHC can be imbeded into the bones of the Guizhou minipig securely and used repeatedly in the same minipig. Conclusion: BHC can be loaded kinds of bone graft materials and can be imbeded into the bones of the Guizhou minipig. The histocompatibility of the BHC is very well, which can be used repeatedly, and the operation is simple.
Objective:(1)We create the bone harvest chamber(BHC), then verify the possibility that if it can be imbedded into the bones of the Guizhou minipig and used repeatedly in the same minipig. Methods:(1) We designed and made the BHC according to the anatomical data of the humerus and femur of GuiZhou minipigs. (2)Two Guizhou minipig were used. The BHC which were loaded the bone graft materials were imbedded into humeral and femoral metaphysis of the Guizhou minipigs. Checked them by imaging detection at postoperative 2 weeks,and harvested chambers at postoperative 4 weeks while imbeded new chambers. Results:1、The dimension of the BHC is suitable.2、BHC can be imbeded into the bones of the Guizhou minipig securely and used repeatedly in the same minipig. Conclusion: BHC can be loaded kinds of bone graft materials and can be imbeded into the bones of the Guizhou minipig. The histocompatibility of the BHC is very well, which can be used repeatedly, and the operation is simple.
引文
1. Charlotte Jeppsson, Mathias Bostrom, Per Aspenberg.Intraosseous BMP implants in rabbits inhibitory effect on bone formation.Acta Orthop Scand[J]1999;70(1):77-83
2. Charlotte Jeppsson, Jorgen Astrand, Magnus Tagil,et al. A combination of bisphosphonate and BMP additives in impacted bone allografts. Acta Orthop Scand[J]2003;74(4):483-489
3. Hanspeter Frei, John O'Connell, Bassam A. Masri,et al. Biological and mechanical changes of the bone graft-cement interface after impaction allografting. Journal of Orthopaedic Research[J] 2005;23:1271-1279
4. Robert L. Vonau, DVM, Mathias P. G. Bostrom,et al. Combination of Growth Factors Inhibits Bone Ingrowth in the Bone Harvest Chamber. Clinical Orthopaedics and Related Research[J]2001;386:243-251
5. Duyck J, Slaets E, Sasaguri K,et al. Effect of intermittent loading and surface roughness on peri-implant bone formation in a bone chamber model. Journal of Clinical Peridontology[J]2007;34:998-1006
6. Magnus Tagil, Per Aspenberg. Fibrous tissue armoring increases the mechanical strength of an impacted bone graft. Acta Orthop Scand[J]2001; 72 (1):78-82
7. J. S. Wangl, J. Diaz2, A. Sabokbar,et al. In vitro and in vivo biological responses to a novel radiopacifying agent for bone cement. Journal of The Royal Society Interface[J]2005;2:71-78
8. Michael C. D. Trindade,l Yong Song, Per Aspenberg,et al. Proinflammatory Mediator Release in Response to Particle Challenge:Studies Using the Bone Harvest Chamber. J Biomed Mater Res (Appl Biomater) 1999;48:434-439
9. Nancy M.P. Lamerigts, MD, PhD,et al. Role of Growth Factors in the Incorporation of Unloaded Bone Allografts in the Goat. Clinical Orthopaedics and Related Research[J]1999;368:260-270
10. Ralf Skripitzl, Troels T Andreassen,Per Aspenberg. Strong effect of PTH (1-34) on regenerating bone. Acta Orthop Scand[J] 2000; 71 (6):619-624
11. Nancy Lamerigts, Per Aspenberg,Pieter Buma,et al. The Repeated Sampling Bone Chamber:A New Permanent Titanium Implant to Study Bone Grafts in the Goat.Laboratory Animal Science[J]1997;47(4):401-406
12. Joke Duyck, Michel De Cooman, Robert Puers,et al. A repeated sampling bone chamber methodology for the evaluation of tissue differentiation and bone adaptation around titanium implants under controlled mechanical conditions,. Journal of Biomechanics[J]2004;37:1819-1822
13. Katleen Vandamme,Ignace Naert,Liesbet Geris.et al. Histodynamics of bone tissue formation around immediately loaded cylindrical implants in the rabbit. Clin. Oral Impl. Res.[J] 2007;18:471-480
1.PietrzakWS,WoodellMJ,McDonald N. Assay of bonemorphogenetic protein22,24, and 27 in human demineralized bone matrix[J]. Cran Surg,2006,17(1):84-90.
2.王玉学,李郑民,毕郑刚.建立兔桡骨缺损动物模型的实验研究[J].中国地方病防治杂志,2008,23(1)：60-61
3. Kasten P,Luginbuhl R, van GriensvenM, et al. Comparison of human bone marrow stromal cells seeded on calcium2deficient hydroxyapa2 tite, β 2tricalcium phosphate and demineralized bone matrix[J]. Bio2 materials,2003,24 (15):2593-2603.
4.李建军,王文军,韩冬,等.转染骨形态发生蛋白-2基因的人骨髓间质干细胞复合异种骨支架异位成骨的效果[J].中华创杂志,2004,20(6)：347-350
5. Uwe Klammert,Joachim Nickel,Kristian Wurzler,et al. Biological activity of a genetically modified BMP-2 variant with inhibitory activity[J].Head & Face Medicine,2009,5(6):234-239
6.李雪盛,孙建军.脱细胞松质骨复合骨形态发生蛋白2异位成骨的实验研究[J],生物医学工程与临床,2009,13(4)：275-278
7.强辉,张坤正,张明宇,等.B一磷酸三钙复合成骨细胞的异位成骨[J].中国组织工程研究与临床康复,2009,13(12)：2300-2304
8.陈顺广,郑启新,郭晓东,等.TGF—β1基因转染骨髓基质干细胞促进BMP-2活性多肽大鼠体内异位成骨[J].华中科技大学学报(医学版),2009,38(1)：44-48
9.黄颖利,程维平,马东洋.基于脂肪干细胞和藻酸钙凝胶构建可注射性骨的实验研究[J].中国美容医学,2009,18(7)：967-969
10.林崇韬,李红艳,闫嘉晴.复合膜异位诱导成骨研究[J].华西口腔医学杂志,2009,27(4)：357-360
11.田晓滨,孙立,杨述华,等.基因活化纳米骨浆异位诱导成骨能力的实验观察[J].中华骨科杂志,2007,27(8)：604-608
12.张森林,毛天球.应用富血小板血浆和骨髓基质细胞构建细胞-膜片及其异位成 骨能力的实验研究[J].临床口腔医杂志,2009,25(3)：147-150
13. Johnson EE, UristMR, Schmalzried TP, et al.Autogeneic cancellous bone grafts in extensive segmental ulnar defects in dogs 1 Effects of xe-nogeneic bovine bone morphogenetic protein without and with interpo-sition of soft tissues and interruption of blood supply[J]. Clin Orthop Relat Res,1989,243:254-265.
14. Cowan CM, Aghaloo T, Chou YF, et al. MicroCT evaluation of Three-Dimensional mineralization in response to BMP-2 doses in vitro and in critical sized rat calvarial defects [J]. Tissue Eng,2007,13 (3):501-512.
15.荣小芳,吴琳,杨晓东,等.兔下颌骨临界骨缺损人工材料植入实验动物模型的建立[J].中国医科大学学报,2008,37(1)：62-37
16.秦辉,许建中,王序全,等.组织工程用大段负重骨缺损动物模型的建立[J].中国临床康复,2004,8(20)：3974-3975
17.谢建雅,周磊,郭毅,利用兔胫骨干骺端骨缺损模型对骨代用品研究的实验方法探讨[J].临床口腔医学杂志,2009,25(6)：329-331
18.张元平,崔继秀,裴国献,等.兔股骨缺损模型的建立[J].中国临床康复,2006,10(5)：87-89
19.田晓滨,孙立,杨述华,等.骨形态发生蛋白-2基因活化纳米骨浆修复兔桡骨缺损的实验研究[J].中华创伤骨科杂志,2007,9(12)：1165-1169
20. Magnus Tagil,Per Aspenberg. Fibrous tissue armoring increases the mechanical strength of an impacted bone graft[J]. Acta Orthop Scand 2001; 72 (1):78-82
21. Nancy Lamberigts,Per Aspenberg,Pieter Buma et al.The repeated sampling bone chamber:a new permanent titanium implant to study bone grafts in the goat[J].Laboratory Animal Science,1997,47(4):401-406
22. Joke Duyck, Michel De Cooman, Robert Puers,et al. A repeated sampling bone chamber methodology for the evaluation of tissue differentiation and bone adaptation around titanium implants under controlled mechanical conditions[J].2004(37):1819-1822
23. Hanspeter Frei, John O'Connell, Bassam A. Masri,et al. Biological and mechanical changes of the bone graft-cement interface after impaction allografting[J]. Journal of Orthopaedic Research,2005(23):1271-1279
24. Charlotte Jeppsson, Jorgen Astrand, Magnus Tagil,et al. A combination of bisphosphonate and BMP additives in impacted bone allografts[J]. Acta Orthop Scand 2003; 74 (4):483-489
25. J. S. Wang, J. Diaz, A. Sabokbar,et al. In vitro and in vivo biological responses to a novel radiopacifying agent for bone cement[J].Journal of The Royal Society Interface,2005(2):71-78
2. Charlotte Jeppsson, Jorgen Astrand, Magnus Tagil,et al. A combination of bisphosphonate and BMP additives in impacted bone allografts. Acta Orthop Scand[J]2003;74(4):483-489
3. Hanspeter Frei, John O'Connell, Bassam A. Masri,et al. Biological and mechanical changes of the bone graft-cement interface after impaction allografting. Journal of Orthopaedic Research[J] 2005;23:1271-1279
4. Robert L. Vonau, DVM, Mathias P. G. Bostrom,et al. Combination of Growth Factors Inhibits Bone Ingrowth in the Bone Harvest Chamber. Clinical Orthopaedics and Related Research[J]2001;386:243-251
5. Duyck J, Slaets E, Sasaguri K,et al. Effect of intermittent loading and surface roughness on peri-implant bone formation in a bone chamber model. Journal of Clinical Peridontology[J]2007;34:998-1006
6. Magnus Tagil, Per Aspenberg. Fibrous tissue armoring increases the mechanical strength of an impacted bone graft. Acta Orthop Scand[J]2001; 72 (1):78-82
7. J. S. Wangl, J. Diaz2, A. Sabokbar,et al. In vitro and in vivo biological responses to a novel radiopacifying agent for bone cement. Journal of The Royal Society Interface[J]2005;2:71-78
8. Michael C. D. Trindade,l Yong Song, Per Aspenberg,et al. Proinflammatory Mediator Release in Response to Particle Challenge:Studies Using the Bone Harvest Chamber. J Biomed Mater Res (Appl Biomater) 1999;48:434-439
9. Nancy M.P. Lamerigts, MD, PhD,et al. Role of Growth Factors in the Incorporation of Unloaded Bone Allografts in the Goat. Clinical Orthopaedics and Related Research[J]1999;368:260-270
10. Ralf Skripitzl, Troels T Andreassen,Per Aspenberg. Strong effect of PTH (1-34) on regenerating bone. Acta Orthop Scand[J] 2000; 71 (6):619-624
11. Nancy Lamerigts, Per Aspenberg,Pieter Buma,et al. The Repeated Sampling Bone Chamber:A New Permanent Titanium Implant to Study Bone Grafts in the Goat.Laboratory Animal Science[J]1997;47(4):401-406
12. Joke Duyck, Michel De Cooman, Robert Puers,et al. A repeated sampling bone chamber methodology for the evaluation of tissue differentiation and bone adaptation around titanium implants under controlled mechanical conditions,. Journal of Biomechanics[J]2004;37:1819-1822
13. Katleen Vandamme,Ignace Naert,Liesbet Geris.et al. Histodynamics of bone tissue formation around immediately loaded cylindrical implants in the rabbit. Clin. Oral Impl. Res.[J] 2007;18:471-480
1.PietrzakWS,WoodellMJ,McDonald N. Assay of bonemorphogenetic protein22,24, and 27 in human demineralized bone matrix[J]. Cran Surg,2006,17(1):84-90.
2.王玉学,李郑民,毕郑刚.建立兔桡骨缺损动物模型的实验研究[J].中国地方病防治杂志,2008,23(1)：60-61
3. Kasten P,Luginbuhl R, van GriensvenM, et al. Comparison of human bone marrow stromal cells seeded on calcium2deficient hydroxyapa2 tite, β 2tricalcium phosphate and demineralized bone matrix[J]. Bio2 materials,2003,24 (15):2593-2603.
4.李建军,王文军,韩冬,等.转染骨形态发生蛋白-2基因的人骨髓间质干细胞复合异种骨支架异位成骨的效果[J].中华创杂志,2004,20(6)：347-350
5. Uwe Klammert,Joachim Nickel,Kristian Wurzler,et al. Biological activity of a genetically modified BMP-2 variant with inhibitory activity[J].Head & Face Medicine,2009,5(6):234-239
6.李雪盛,孙建军.脱细胞松质骨复合骨形态发生蛋白2异位成骨的实验研究[J],生物医学工程与临床,2009,13(4)：275-278
7.强辉,张坤正,张明宇,等.B一磷酸三钙复合成骨细胞的异位成骨[J].中国组织工程研究与临床康复,2009,13(12)：2300-2304
8.陈顺广,郑启新,郭晓东,等.TGF—β1基因转染骨髓基质干细胞促进BMP-2活性多肽大鼠体内异位成骨[J].华中科技大学学报(医学版),2009,38(1)：44-48
9.黄颖利,程维平,马东洋.基于脂肪干细胞和藻酸钙凝胶构建可注射性骨的实验研究[J].中国美容医学,2009,18(7)：967-969
10.林崇韬,李红艳,闫嘉晴.复合膜异位诱导成骨研究[J].华西口腔医学杂志,2009,27(4)：357-360
11.田晓滨,孙立,杨述华,等.基因活化纳米骨浆异位诱导成骨能力的实验观察[J].中华骨科杂志,2007,27(8)：604-608
12.张森林,毛天球.应用富血小板血浆和骨髓基质细胞构建细胞-膜片及其异位成 骨能力的实验研究[J].临床口腔医杂志,2009,25(3)：147-150
13. Johnson EE, UristMR, Schmalzried TP, et al.Autogeneic cancellous bone grafts in extensive segmental ulnar defects in dogs 1 Effects of xe-nogeneic bovine bone morphogenetic protein without and with interpo-sition of soft tissues and interruption of blood supply[J]. Clin Orthop Relat Res,1989,243:254-265.
14. Cowan CM, Aghaloo T, Chou YF, et al. MicroCT evaluation of Three-Dimensional mineralization in response to BMP-2 doses in vitro and in critical sized rat calvarial defects [J]. Tissue Eng,2007,13 (3):501-512.
15.荣小芳,吴琳,杨晓东,等.兔下颌骨临界骨缺损人工材料植入实验动物模型的建立[J].中国医科大学学报,2008,37(1)：62-37
16.秦辉,许建中,王序全,等.组织工程用大段负重骨缺损动物模型的建立[J].中国临床康复,2004,8(20)：3974-3975
17.谢建雅,周磊,郭毅,利用兔胫骨干骺端骨缺损模型对骨代用品研究的实验方法探讨[J].临床口腔医学杂志,2009,25(6)：329-331
18.张元平,崔继秀,裴国献,等.兔股骨缺损模型的建立[J].中国临床康复,2006,10(5)：87-89
19.田晓滨,孙立,杨述华,等.骨形态发生蛋白-2基因活化纳米骨浆修复兔桡骨缺损的实验研究[J].中华创伤骨科杂志,2007,9(12)：1165-1169
20. Magnus Tagil,Per Aspenberg. Fibrous tissue armoring increases the mechanical strength of an impacted bone graft[J]. Acta Orthop Scand 2001; 72 (1):78-82
21. Nancy Lamberigts,Per Aspenberg,Pieter Buma et al.The repeated sampling bone chamber:a new permanent titanium implant to study bone grafts in the goat[J].Laboratory Animal Science,1997,47(4):401-406
22. Joke Duyck, Michel De Cooman, Robert Puers,et al. A repeated sampling bone chamber methodology for the evaluation of tissue differentiation and bone adaptation around titanium implants under controlled mechanical conditions[J].2004(37):1819-1822
23. Hanspeter Frei, John O'Connell, Bassam A. Masri,et al. Biological and mechanical changes of the bone graft-cement interface after impaction allografting[J]. Journal of Orthopaedic Research,2005(23):1271-1279
24. Charlotte Jeppsson, Jorgen Astrand, Magnus Tagil,et al. A combination of bisphosphonate and BMP additives in impacted bone allografts[J]. Acta Orthop Scand 2003; 74 (4):483-489
25. J. S. Wang, J. Diaz, A. Sabokbar,et al. In vitro and in vivo biological responses to a novel radiopacifying agent for bone cement[J].Journal of The Royal Society Interface,2005(2):71-78