益气生津法抗体力性疲劳作用机理研究
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摘要
气与津液的健运是机体运动能力的物质基础,气属阳,津液属阴,二者相辅相成,相互生化。气与津液的正常生成可以直接影响机体能否迅速修复运动性疲劳所造成的基质的生理化学状态改变,并补充活动所造成的基质消耗;而气的运行与津液的生成、输布和排泄又是基质疲劳代谢产物排除和恢复机体调节和协调机能的重要条件。
目的:研究益气生津法的理论依据及其立法方药,证实益气生津法抗疲劳作用的有效性,并对其作用机理进行深入的研究。
方法:实验一:Wistar大鼠用水和低、中、高三个不同剂量的益气生津方喂养八周,其中的运动疲劳模型组从第五周开始进行递增时间游泳训练4周,对正常和运动疲劳大鼠能量物质代谢指标、血液、免疫和内分泌系统功能的影响进行检测。实验二:Wistar大鼠用水和中、高两个不同剂量的益气生津方喂养4周,其中的运动疲劳模型组同时进行递增时间游泳训练4周,观察对大鼠力竭游泳时间的影响
结果:益气生津方可显著延长运动疲劳大鼠力竭游泳时间(p＜0.05),提高胰高血糖素水平(p＜0.01),降低皮质酮水平(p＜0.01),帮助保持血糖水平,降低血清游离脂肪酸水平(p＜0.05),降低血清D-3羟丁酸和尿素氮水平(p＜0.01),升高血红蛋白含量及红细胞总数和红细胞压积(p＜0.05),提高CD4、IgG、和IgM水平(p＜0.05)、NK细胞活性(p＜0.05),并在运动疲劳模型组显著降低IL-6应激性的升高(p＜0.05)。
结论:本研究证实益气生津法可以延长力竭游泳时间,是抗体力性疲劳的有效方法。其作用机理为:①提高胰高血糖素水平,帮助机体有效地利用糖和脂肪作为能量来源;②增加红细胞水半和血液携氧能力,增加肌肉供氧量;③促进代谢产物消除,延缓肌疲劳产生;④降低IL-6和皮质酮水平,减少机体对运动疲劳的应激反应及损伤;⑤改善运动疲劳机体的部分特异和非特异免疫功能,提高机体免疫力。
BACKGROUND:The health of Oi and fluid forms the material foundation for physical performance.Qi belongs to yang while fluid belongs to yin.They are an interdependent pair that produces each other and transforms into each other.Their normal production can directly impact the body's capability to quickly regain balance and replenish energy material lost during physical exertion.The movement of qi and the production,distribution and elimination of fluid are necessary for the removal of waste material which is also an important factor in fatigue recovering mechanism.PURPOSE:The purpose of study was to define the Chinese medicine theorilogical basis for using Enhancing Qi while Nourishing Fluid(EQNF) method against physical fatigue and the EQNF formula,and to study the effectiveness and mechanism of EQNF formula on physical fatigue prevention.METHODS: Wistar Rats was used in both studies.StudyⅠ:EQNF formula was given to both control group and fatigue animal model group at three doseage levels while water was given to both zero dose control group for 8 weeks.After 4 work of free living,fatigue anminal model group went through four weeks swim training regime.Indicators on energy material metabolism,and the changes in blood,immume,and endocrine system were measured.StudyⅡ:EQNF formula was given to both control group and fatigue animal model group at two doseage levels while water was given to both zero dose control group for 4 weeks. Fatigue anminal model group went through four weeks swim training regime.Swimming to exhaustion time was measured on fatigue animal model group at 2.5 weeks and 4 week and on control group at at 4 weeks.RESULTS:EQNF formula can prolong the swimming to exhaustion time(p＜0.05);increase serum glucagon(p＜0.01);reduce serum corticosterone(p＜0.01);help maintain blood glucose level;reduce serum free fatty acid(p＜0.05),D-3-hydroxybutyric acid and blood urea nitrogen(p＜0.01);increase red blood cell count and Haemoglobin level(p＜0.05);increase CD4,IgG and IgM level(p＜0.05);activates nature killer cells(p＜0.05);and lowers IL-6 elevation in low and high dose fatigue animal model group(p＜0.05).CONCLUSIONS:EQNF formula could improve rats' capability to endure physical stress through 1) increase glucagon secretion to maintain high blood sugar level and using more body fat as energy source during exercise;2) increase HGB and RBC level to improve oxygen supply to muscle tissue; 3)improve the elimination of waste material to delay the onset of muscle fatigue;4) slow down stress reaction caused by physical stress;5) increase certain specific and non-specific immune functions in fatigued animals.
目的:研究益气生津法的理论依据及其立法方药,证实益气生津法抗疲劳作用的有效性,并对其作用机理进行深入的研究。
方法:实验一:Wistar大鼠用水和低、中、高三个不同剂量的益气生津方喂养八周,其中的运动疲劳模型组从第五周开始进行递增时间游泳训练4周,对正常和运动疲劳大鼠能量物质代谢指标、血液、免疫和内分泌系统功能的影响进行检测。实验二:Wistar大鼠用水和中、高两个不同剂量的益气生津方喂养4周,其中的运动疲劳模型组同时进行递增时间游泳训练4周,观察对大鼠力竭游泳时间的影响
结果:益气生津方可显著延长运动疲劳大鼠力竭游泳时间(p＜0.05),提高胰高血糖素水平(p＜0.01),降低皮质酮水平(p＜0.01),帮助保持血糖水平,降低血清游离脂肪酸水平(p＜0.05),降低血清D-3羟丁酸和尿素氮水平(p＜0.01),升高血红蛋白含量及红细胞总数和红细胞压积(p＜0.05),提高CD4、IgG、和IgM水平(p＜0.05)、NK细胞活性(p＜0.05),并在运动疲劳模型组显著降低IL-6应激性的升高(p＜0.05)。
结论:本研究证实益气生津法可以延长力竭游泳时间,是抗体力性疲劳的有效方法。其作用机理为:①提高胰高血糖素水平,帮助机体有效地利用糖和脂肪作为能量来源;②增加红细胞水半和血液携氧能力,增加肌肉供氧量;③促进代谢产物消除,延缓肌疲劳产生;④降低IL-6和皮质酮水平,减少机体对运动疲劳的应激反应及损伤;⑤改善运动疲劳机体的部分特异和非特异免疫功能,提高机体免疫力。
BACKGROUND:The health of Oi and fluid forms the material foundation for physical performance.Qi belongs to yang while fluid belongs to yin.They are an interdependent pair that produces each other and transforms into each other.Their normal production can directly impact the body's capability to quickly regain balance and replenish energy material lost during physical exertion.The movement of qi and the production,distribution and elimination of fluid are necessary for the removal of waste material which is also an important factor in fatigue recovering mechanism.PURPOSE:The purpose of study was to define the Chinese medicine theorilogical basis for using Enhancing Qi while Nourishing Fluid(EQNF) method against physical fatigue and the EQNF formula,and to study the effectiveness and mechanism of EQNF formula on physical fatigue prevention.METHODS: Wistar Rats was used in both studies.StudyⅠ:EQNF formula was given to both control group and fatigue animal model group at three doseage levels while water was given to both zero dose control group for 8 weeks.After 4 work of free living,fatigue anminal model group went through four weeks swim training regime.Indicators on energy material metabolism,and the changes in blood,immume,and endocrine system were measured.StudyⅡ:EQNF formula was given to both control group and fatigue animal model group at two doseage levels while water was given to both zero dose control group for 4 weeks. Fatigue anminal model group went through four weeks swim training regime.Swimming to exhaustion time was measured on fatigue animal model group at 2.5 weeks and 4 week and on control group at at 4 weeks.RESULTS:EQNF formula can prolong the swimming to exhaustion time(p＜0.05);increase serum glucagon(p＜0.01);reduce serum corticosterone(p＜0.01);help maintain blood glucose level;reduce serum free fatty acid(p＜0.05),D-3-hydroxybutyric acid and blood urea nitrogen(p＜0.01);increase red blood cell count and Haemoglobin level(p＜0.05);increase CD4,IgG and IgM level(p＜0.05);activates nature killer cells(p＜0.05);and lowers IL-6 elevation in low and high dose fatigue animal model group(p＜0.05).CONCLUSIONS:EQNF formula could improve rats' capability to endure physical stress through 1) increase glucagon secretion to maintain high blood sugar level and using more body fat as energy source during exercise;2) increase HGB and RBC level to improve oxygen supply to muscle tissue; 3)improve the elimination of waste material to delay the onset of muscle fatigue;4) slow down stress reaction caused by physical stress;5) increase certain specific and non-specific immune functions in fatigued animals.
引文
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