摘要
基因诊断已经成为分子生物学和生物医学研究中的重要领域。人体、病毒和细菌核酸中特定碱基序列的检测在疾病诊断、食品污染、法医鉴定和环境监测等领域都将会发挥越来越重要的作用。大规模的基因检测要求建立更简便、迅速、廉价、微型化的分析装置。许多新的生物技术的开发,为发展高灵敏度、高特异性的基因分析检测方法注入了活力,其中利用DNA双链的碱基互补配对原则发展起来的各种DNA生物传感技术,受到生物分析工作者的高度重视。电化学DNA检测方法以其灵敏度高、轻巧便宜、携带方便、耗能少、能与现代微电子技术联用,易于实现微型化等优点,受到了研究者们的广泛关注,俨然成为当今生物学、医学领域的前沿性课题。
随着人类基因组计划(HGP)的顺利完成,生命科学从确定核酸(DNA、RNA)序列层次深入到功能基因组研究和蛋白质组学研究上来。如何构建高速度、高通量、集约化、有特异性的高灵敏蛋白质检测技术是目前蛋白质组学研究所面临的紧迫任务。传统蛋白质的检测主要利用抗体一抗原的特异相互作用。利用寡核苷酸间的严格的识别和亲和力而设计的人工合成寡核苷酸—适体(aptamer)的出现,使抗体抗原反应发生新的革命性变化,弥补了现有抗体的不足。也为传统免疫传感器发展开辟了一条新的道路。核酸适体对蛋白质的结合力和特异性可与抗原抗体间的作用力相媲美,且与抗体相比有许多优越性。因此利用核酸适体构建蛋白质的电化学检测方法已引起许多科学工作者的关注。
蛋白质和核酸是组成生命的主要生物大分子,核酸具有传递遗传信息等功能,而蛋白质则贯穿所有的生命活动过程。有关核酸与蛋白质之间的相互作用的信息的获得,将有助于对一些生命过程的的研究,进而掌握生命活动和信息传递规律并实施调节及调控。
纳米技术的出现为纳米材料在分析化学领域的发展和应用开辟了新的方向。纳米材料的优异性能例如比表面积大、反应活性高等为分析化学开辟了新的研究途径,纳米粒子的独特性能为生物检测奠定了基础,其和核酸适体的高度特异性,电化学方法的高灵敏性相结合,使得检测的灵敏度大大提高,使其应用范围更加广阔,将为生物分析化学开辟新的领域。
本论文的目的就是研究基于纳米颗粒的新型电化学检测方法。利用纳米金的优异的电化学性能和纳米磁性颗粒的顺磁性,设计了几种新型的电化学DNA和凝血酶蛋白质检测方法,拓宽了后基因时代的研究领域,论文主要内容如下:
第一章绪论
首先介绍了DNA生物传感器和核酸适体生物传感器的原理、应用及其研究进展,其中着重介绍了电化学的DNA生物传感器的原理和研究进展,重点介绍了核酸适体生物传感器的原理和应用,对今后的发展方向和趋势进行了展望。接着介绍了纳米材料在分析化学中的应用。最后阐述了本论文的目的和意义。
第二章基于核酸适体和纳米材料的凝血酶蛋白电化学特异性识别的研究
本文介绍了一种结合核酸适体技术和纳米技术,以凝血酶蛋白为研究对象的高效、高灵敏、特异性识别蛋白质的电化学方法。利用金纳米颗粒标记的核酸适体以及被固定在磁性纳米颗粒上的核酸适体与凝血酶蛋白同时结合形成磁性颗粒/凝血酶/纳米金胶的三明治结构,利用磁性分离,将金胶纳米颗粒特异性地吸着到电极表面,通过检测电极上金胶的电化学信号,实现对凝血酶靶蛋白的检测。该方法对凝血酶蛋白具有很高的特异性识别能力,其检测不受其他蛋白质如牛血清白蛋白等存在的干扰,可应用于实际血浆中凝血酶的检测。由于利用磁性纳米颗粒使得分离、富集和测定在同一个自制的电化学反应池中进行,其操作不仅简单,而且检测的灵敏度得到提高。该蛋白质检测的线性范围为5.6×10~(-12)mol/L~1.12×10~(-9)mol/L,检测限可以达到1.42×10~(-12)mol/L。该方法对凝血酶蛋白有很好的特异性,其他蛋白如溶菌酶和牛血清白蛋白的存在对于检测没有影响。
第三章利用互补核酸杂交富集金胶实现信号扩增的凝血酶蛋白质的特异性电化学检测研究
在本文中,介绍了一种利用互补核酸杂交富集金胶实现信号扩增的特异性蛋白检测研究。以凝血酶蛋白为研究对象,利用凝血酶蛋白相对应的二段核酸适体,适体Ⅰ固定在磁性颗粒上用于特异性地捕获蛋白,适体Ⅱ标记金胶作为检测信标。由凝血酶蛋白和相对应的二段核酸适体构建三明治结构的特异性识别凝血酶的蛋白检测方法。另外,再通过信标金胶上过剩的核酸适体链与另一段标记有金胶的互补核酸进一步杂交,获得金胶的选择性聚集,实现信号扩增。通过信号扩增,此传感器的灵敏度大大提高,对凝血酶蛋白的检测下限可以达到4.52×10~(-15)mol/L。平行测定浓度为7.47×10~(-14)mol/L的凝血酶八次,其RSD为3.0%。
第四章基于硫代三聚氢酸和金纳米颗粒形成网状结构实现信号扩增的超灵敏凝血酶蛋白质电化学检测的研究
在本文中,介绍了一种利用金纳米颗粒和硫代三聚氢酸来实现信号扩增的超灵敏度和高度特异性的蛋白质的电化学检测方法。以凝血酶蛋白为研究对象,由凝血酶蛋白和相对应的二段核酸适体构建三明治结构的凝血酶蛋白特异性检测方法的基础上,再通过硫代三聚氢酸和金纳米颗粒的自组装作用,形成金纳米颗粒和硫代三聚氢酸的网状结构,获得金纳米颗粒的选择性聚集,实现信号扩增。通过信号扩增,该方法的灵敏度大大提高,对凝血酶蛋白的检测下限可以达到7.82 amol/L。该电化学检测方法对凝血酶蛋白有很好的特异性,其他蛋白如溶菌酶和牛血清白蛋白对于检测没有影响。可望用于其他蛋白的检测,疾病的诊断和其他的生物研究领域。
第五章基于金纳米颗粒选择性聚集实现信号扩增特异性电化学检测p53基因的研究
在本文中,介绍了一种利用金纳米颗粒的聚集实现信号放大作用的超灵敏和高度特异性的电化学方法用于人类p53肿瘤抑制剂基因研究。在实验中,根据p53基因的序列设计并合成了能特异性检测p53肿瘤抑制剂基因的二段探针,在一段探针上固定磁性颗粒以捕获并富集目标DNA,在另一段探针上标记金纳米颗粒作为检测信标。另外,再通过硫代三聚氢酸和金纳米颗粒的自组装作用,形成金纳米颗粒和硫代三聚氢酸的网状结构,获得金纳米颗粒的选择性聚集,实现信号扩增。用此法检测得到的目标野生型DNA,最低检测限为2.24×10~(-17)mol/L。在同样浓度的条件下,单碱基错配的突变型序列和完全互补的野生型序列的区分度为57.1%。
第六章基于纳米金胶和磁性纳米颗粒和利用取代反应电化学特异性识别凝血酶蛋白质的研究
本文介绍了一种基于取代反应的特异性识别凝血酶的电化学方法。利用金胶标记的互补核酸和固定在磁性纳米颗粒上的核酸适体的杂交,形成双链的核酸适体/互补核酸,在凝血酶蛋白的存在下,核酸适体与凝血酶蛋白特异性的结合,形成G-四分体的结构,使得原来的双链解离,标记有金胶的互补核酸游离出来,通过检测游离的互补核酸上标记的金的电化学信号,实现对凝血酶蛋白的特异性检测,其最低检测限可达1.22×10~(-11)mol/L。该方法十分简单有效,无需对蛋白进行标识,为蛋白质的检测提供了一种新的思路。
第七章利用目标蛋白存在下的取代反应研究核酸适体-互补核酸和核酸适体-蛋白之间结合的热力学特性
本文介绍了一种基于目标蛋白的取代反应来研究核酸适体-互补核酸和核酸适体-目标蛋白之间的相互作用,以凝血酶蛋白为研究对象,比较了凝血酶蛋白的两段核酸适体,核酸适体Ⅰ(aptamerⅠ)和核酸适体Ⅱ(aptamerⅡ)与互补核酸和目标蛋白作用的情况,并确定了核酸适体-互补核酸的解离反应和在目标蛋白存在下取代反应的平衡常数、标准焓变和标准熵变等热力学参数,结果表明该取代反应是一个熵驱动的自发过程,熵驱动从双链的核酸转变为核酸适体-目标蛋白的复合物。核酸适体与目标蛋白的特异性结合是一个放热过程。该特异性结合的主要作用是核酸适体与凝血酶之间的高度的分子构型的匹配、氢键力和沟槽作用。该热力学研究会对核酸适体-互补核酸和核酸适体-目标蛋白之间的结合过程的机理有一个更深的理解,将有助于我们进一步揭示核酸与蛋白这两种生命中最关键物质之间的相互作用和关系,对于我们更好地理解基本的生物过程和预测设计适体生物检测方法,发展用于疾病诊断的方法有着重要的意义。
With the development of the Human Genome Project, Gene diagnosis has become an important area in molecular biology and biomedical research. The detection of specific DNA sequence in human body, viral and bacteria nucleic acid is playing a more and more important role in disease diagnose, food pollution, legal medical examination and environment detection. Wide-scale genetic testing requires the development of easy-to-use, fast, inexpensive, miniaturized devices. Many new biological technologies emerged and found their applications in this field. Among them, DNA detection technologies relying on the DNA base-pair recognition are rapidly developed and have received considerable attentions. Electrochemical DNA detection is a novel and developing technique that combining biochemical, electrochemical, medical and electronic techniques with the advantages of being simple, reliable, cheap, sensitive and selective for genetic detection, and has been a hot topic in the field of biochemistry and medicine.
With the accomplishment of Human Genome Project and the rapid progress of proteomic strategies, protein detection has been a topic of significant interest. Intense research activities are carried out worldwide to develop rapid, simple, specific and sensitive detection devices for protein in medical diagnostics and biomedical research application, while the detection methodologies for protein based on antibodies cannot meet the demand of the human proteome. Now aptamers have been emerging as a new protein recognition element in wide range of bioassays. Aptamers have attracted a considerable attention due to their ability to bind target protein with high affinity and specificity and have many advantages over antibodies, including simpler synthesis, easier storage, reproducibility, and wider applicability. These properties make aptamers ideal candidates as protein recognition elements in a wide range of bioassays and for the development of diseases diagnostics.
The emergence of nanotechnology is opening new horizons for the application of nanoparticles in analytical chemistry. Nanoparticles are of considerable interest owing to their unique physical and chemical properties, and offer excellent prospects for biosensor. The power and scope of such nanoparticles can be greatly enhanced by coupling them with the high specificity of the aptamer and the high sensitivity of the electrochemical recognitions.
Nucleic acid and protein are the most important biomolecules in life. Nucleic acid is in charge of transferring genetic information and coding other biomolecules, while protein has run through the whole life process. The investigation on the interaction of the nucleic acids and protein has been one of the most important areas. The information of the detailed and fundamental nature of the interaction obtained will help to understand the organization of biological systems and contribute to control and adjust the life process.
The goal of the present study is to design and optimize new electrochemical techniques with high sensitivity and selectivity. This paper combines the excellent characteristics of nanoparticles, base-complementary and the specific recognition of protein. The high sensitivity of electrochemical device coupled to their compatibility with modem micro-fabrication technologies, portability, low cost, minimal power requirements, and independence of sample turbidity, made them excellent candidates for DNA and protein detection and also it would broaden the research field of post-genetic time. The dissertation includes seven parts: Chapter One: introduction
In the beginning of this dissertation, the basic composition of a DNA biosensor and its classification were systematically reviewed, especially the design of a DNA electrochemical biosensor was introduced, together with the application and development of DNA biosensor. Secondly, we introduced the concept of the aptamer, emphatically reviewed the operational principle, classification, application and development trends of aptamer biosensor. Thirdly, nanoparticles were introduced, especially the application of gold nanoparticles and magnetic nanoparticles in the biosensor was presented. At last, the purpose and the significance of the dissertation were pointed out.
Chapter Two: Study on an electrochemical detection for thrombin based on aptamers and nanoparticles
A high specific, sensitive electrochemical recognition of thrombin based on aptamers and nanoparticles was presented. Two different aptamers were chosen to construct a sandwich manner for detecting thrombin. Aptamer I was immobilized on nano magnetic particle for capturing thrombin, and aptamer II labled with nano gold was used for detection. The electrical current generated from gold after the formation of the complex of magnetic particle, thrombin and nano gold, and then an electrochemical cell designed by ourselves was used for separating, gathering, and electrochemical detecting. Through magnetic separation, high specific and sensitive detection of the target protein, thrombin, was achieved. Linear response was observed over the range 5.6 × 10~(12) mol/L - 1.12 × 10~(-9) mol/L, with a detection limit of 1.42×10~(-12) mol/L. The presence of other protein as BSA did not affect the detection, which indicates that high selective recognition of thrombin can be achieved in complex biological samples such as human plasma.
Chapter Three: An Amplified electrochemical biosensor for thrombin based on the enrichment of gold nanoparticles through the hybridization with the complementary oligonucleotide
An amplified electrochemical biosensor for thrombin based on the enrichment of the gold nanoparticles through the hybridization with the complememtary oligonucleotide was presented. Through the specific recognition for thrombin, a sandwich format of magnetic nanoparticle-aptamerI / thrombin / gold nanoparticle-aptamerII was fabricated. And the signal amplification was further implemented by the enrichment of gold nanoparticles through the hybridization with the gold labeled complementary oligonucleotide, the resulting hybridization is capable of realizing more gold nanoparticle markers attaching for one target protein, thrombin, and hence offer a remarkable amplification of detecting thrombin. A significant sensitivity enhancement was obtained for detection of thrombin with the signal amplification. This new strategy allows the detection of the target protein down to the 4.52 ×10~(-15) mol//L. The relative standard deviation of eight replicate determinations of 7.47×10~(-14) mol/L thrombin was 3.0%.
Chapter Four: A new amplification strategy for ultrasensitive electrochemical detection with network-like thiocyanuric acid/gold nanoparticles
An ultrasensitive and highly specific electrochemical detection for thrombin based on gold nanoparticles and thiocyanuric acid is presented. For this proposed aptasensor, aptamer I was immobilized on the magnetic nanoparticles, aptamer II was labeled with gold nanoparticles. Through the specific recognition for thrombin, a sandwich format of magnetic nanoparticle /thrombin / gold nanoparticle was fabricated, and the signal amplification was further implemented by forming network-like thiocyanuric acid /gold nanoparticles. A significant sensitivity enhancement had been obtained, and the detection limit was down to 7.82 aM. The presence of other proteins such as BSA and lysozyme did not affect the detection of thrombin, which indicates a high specificity of thrombin detection could be achieved. This electrochemical detection is expected to have wide applications in protein monitoring and disease diagnosis.
Chapter Five: An ultrasensitive electrochemical approach for detection of p53 sequence based on the aggregation of gold nanoparticles
A new approach has been developed for the detection of human tumor suppression p53 gene. Two DNA sequences were designed, and the combined sequences are complementary to that of the target p53 sequence, one was immobilized on the magnetic nanoparticles for capturing the target p53 sequence, the other one is labeled with gold nanoparticles as probe, and the sandwich format was formed for detecting the p53 sequences. The ultrasensitive detection was achieved by the aggregation of gold nanoparticles with introducing thiocyanuric acid into the system, and the detection limit could go down to 2.24×10~(-17) mol/L. The mutant type p53 DNA sequence could be obviously distinguished from the wide type p53 DNA sequence. Chapter Six: A displacement assay for protein based on gold nanoparticels and magnetic nanoparticles
A nanoparticle based displacement protocol for detection of thrombin is described. The assay relies on hybridizing the magnetic nanoparticle immobilized aptamer with gold nanoparticles labeled complementary DNA, followed by an incubation assay with the target protein. In the presence of thrombin, aptamer prefers to form G-quarter structure with the target protein, resulting in the release of the gold nanoparticle labeled DNA. A high sensitive detection of thrombin was achieved by the excellent electrochemical signal of gold, and its detection limit could go down to 1.22×10~(-11) mol/L. The assay responds rapidly and specifically to the target protein and
requires no label of the protein and without other reagents.
Chapter Seven: A Thermodynamic investigation into the binding affinities
between aptamer-DNA and aptamer-protein based on the displacement reaction
A thermodynamic investigantion into the binding affinities between aptamer-DNA and aptamer-protein based on the displacement reaction was presented. Some thermodynamic parameters such as the equilibrium constant, enthalpy and entropy of the dissociation reaction and the displacement reaction were evaluated. The results showed that the change of entropy played an important role in the conversion of the duplex DNA into aptamer-protein binding. And it was also deduced that the binding reaction of aptamer toward thrombin is exothermic, its high affinity and specificity are generally achieved by a combination of complementary molecular shapes, hydrogen bonding, and stacking interactions. This has been the first attempt to compare the stability of the duplex DNA and the DNA-protein complex, and it will provide an insight into how the conformation changes. The obtained thermal parameter will direct us to find out the basic principle for designing the aptamer based biosensor and to deeply understand the thermal properties of the aptamer toward protein, and it will be great importance of developing new methods for disease diagnosis.
引文
1. Kelly M Millan, Susan R Mikkelsen. Anal. Chem., 1993, 65(17):2317-2323
2. Watson J D, Crick F H. Nature, 1953,171:137-138
3. Chen Zhangliang, Gu Hongya. Science, 1993, 262(5132):377-378
4. Hood L E, Hunkapiller M W, Smith L M. Genomics, 1987,1(3): 201-202
5. Duan Xinrui, Li Zhengping, He Fang, Wang Shu. J. Am. Chem. Soc, 2007, 129(14):4154-4155
6. Jeng E S, Moll A E, Roy A C, Gastala J B, Strano M S. Nano Lett., 2006, 6(3):371-375
7. Ray P C, Fortner A, Darbha GK.J. Phys. Chem. B., 2006,110(42):20745-20748
8. Najari A, Ho H A, Gravel J.-F., Nobert P, Boudreau D, Leclerc M. Anal. Chem., 2006, 78(22):7896-7899
9. Liu Shili, Li Chao, Cheng Jing, Zhou Yuxiang. Anal. Chem., 2006, 78(13):4722-4726
10. Denise Pollard-Knight, Christopher A Read, Malcolm J Downes, Lesley A Howard, Michael R Leadbetter, Sally A Pheby, Elizabeth McNaughton, Allan Syms and Michael AW Brady. Analytical Biochemistry, 1990,185(1):84-89
11. Polsky R, Gill R, Kaganovsky L, Willner I. Anal. Chem., 2006, 78(7):2268-2271
12. Li Xiaohong, Song Haifeng, Nakatani Kazuhiko, Kraatz Heinz-Bernhard. Anal. Chem., 2007, 79(6):252-2555
13. Lubin A A, Lai R Y, Baker B R, Heeger A J, Plaxco K W. Anal. Chem., 2006, 78(16):5671-5677
14. Arif J M, Dresler C, Clapper M L, Gairola C G, Srinivasan C, Lubet R A, Gupta R C. Chemical Research in Toxicology, 2006,19(2):295-299
15. Bell S E J, Sirimuthu N M S. J. Am. Chem. Soc., 2006,128(49):15580-1581
16.Yeung S S W, Lee T M H, Hsing I.-M. J. Am. Chem. Soc, 2006, 128(41): 13374-13375
17. M.E. Downs, S. Kobayashi, I. Karube. Anal. Lett., 1987, 20(12), 1897-1927
18. Evdokimov I, Skuridin S G, Salianov V I. Mol. Biol. (Mosk), 1989, 23(6), 1581-1588
19. Pfeiffer I, Hook F. Anal. Chem., 2006, 78(21):7493-7498
20. Su X, Lin C.-Y., O'Shea S J, Teh H F, Peh W Y X, Thomsen J S. Anal. Chem., 2006, 78(15)5552-5558
21. Hongbo Su, Krishna M. R. Kallury, Michael Thompson, Arthur Roach. Anal. Chem., 1994, 66(6):769-777
22. Hur Youngjune, Han Jinho, Seon Jooheon, Pak Yukeun Eugene, Roh Yongrae. Sensors and Actuators A: Physical, 2005,120(2):462-467
23. Graham C R, Leslie D, Squirrell D J. Biosensors and Bioelectronics, 1992, 7(7):449-520
24. Gotoh M, Hasegawa Y, Shinohara Y, Shimizu M, Tosu M. DNA Res, 1995, 2(6):285-293
25. Bianchi Nicoletta, Rutigliano Cristina, Tomassetti Marina, Feriotto Giordana, Zorzato Francesco, Gambari Roberto. Clinical and Diagnostic Virology, 1997, 8(3):199-208
26. Monaghan P B, McCarney K M, Ricketts A, Littleford R E, Docherty F, Smith W E, Graham D, Cooper J M. Anal. Chem., 2007, 79(7):2844-2849
27. Boozer C, Ladd J, Chen S, Jiang S. Anal. Chem., 2006, 78(5):1515-1519
28. Liu S, Li C, Cheng J, Zhou Y. Anal. Chem., 2006, 78(13):4722-4726
29. Yang C J, Martinez K, Lin H, Tan W. J. Am. Chem. Soc, 2006, 128(31):9986-9987
30. Kelly M Millan, Angela Saraullo, Susan R Mikkelsen. Anal. Chem., 1994, 66(18):2943-2948
31. Matsumoto Y, Terui N, Tanaka S. Environ. Sci. Technol., 2006, 40(13):4240-4244
32. Maria A A F de C T Carrondo, Miquel Coll, Joan Aymami, Andrew H J Wang, Gijs A Van der Marel, Jacques H. Van Boom, Alexander Rich. Biochemistry, 1989, 28(19):7849-7859
33. Xue D, Elliott C M, Gong P, Grainger D W, Bignozzi C A, Caramori S. J. Am. Chem. Soc, 2007,129(7): 1854-1855
34. Xu Chun, Cai Hong, Xu Qun, He Pingang, Fang Yuzhi. Fresenius' Journal of Analytical Chemistry, 2001, 369(5):428-432
35. Xu Chun, Cai Hong, He Pingang, Fang Yuzhi. Fresenius' Journal of Analytical Chemistry, 2000, 367(6):593-595
36. C.Xu, H.Cai, P.G.He,Y.Z.Fang. Analyst., 2001,126 (1): 62-65;
37.徐春,蔡宏,何品刚,方禹之.高等学校化学学报,2001,22(9):1492-1495
38. Zhu Ningning, Cai Hong, He Pingang, Fang Yuzhi. Analytica Chimica Acta, 2003, 481(2):181-189
39. N.N. Zhu, A.P.Zhang, P.G.He, Y.Z.Fang. Analyst., 2003,128(3),260-264.
40. H.Cai, Q.Wang, P.G.He, Y.Z.Fang. 《高等学校化学学报》, 2003, 24(8), 1390-1394
41. H.Cai, Y.Xu, N.N. Zhu, P.G.He, Y.Z.Fang. Analyst., 2002, 127(6), 803-808
42. Cai Hong, Wang Yanqing, He Pingang, Fang Yuzhi. Analytica Chimica Acta, 2002, 469(2):165-172
43. Cai Hong, Zhu Ningning, Jiang Ying, He Pingang, Fang Yuzhi. Biosensors and Bioelectronics, 2003, 18(11):1311-1319
44. Kavanagh P, Leech D. Anal. Chem., 2006, 78(8):2710-2716
45. Marquette C A, Lawrence M F, Blum L J. Anal. Chem., 2006, 78(3):959-964
46. Xu Ying, Jiang Ying, Cai Hong, He Pingang, Fang Yuzhi. Analytica Chimica Acta, 2004, 516(1-2): 19-27
47. Cai Hong, Xu Ying, He Pingang, Fang Yuzhi. Electroanalysis, 2003, 15 (23-24): 1864-1870
48. Brousseau L C. J. Am. Chem. Soc., 2006, 128(35):11346-11347
49. Tuerk C, Goldberg L. Science, 1990, 249(4968):505-510
50. Nguyen D H, DeFina S C, Fink W H, Dieckmann T. J. Am. Chem. Soc., 2002, 124(50): 15081-15084
51. Jenison Robert D, Gill Stanley C. Science, 263(5152): 1425-1429
52. Shultzaberger R, Schneider T. Nucleic Acids Res., 1999, 27(3):882-87
53. Lee M, Walt D R, Analytical Biochemistry, 2000, 282(1):142-146
54. McCauley Thomas G, Hamaguchi Nobuko, Stanton Martin. Analytical Biochemistry, 2003, 319(2):244-250
55. Davis J J, Tkac J, Laurenson S, Ferrigno P K. Anal. Chem., 2007, 79(3):1089-1096
56. Van Ryk D L, Venkatesan S. J. Biol. Chem., 1999, 274(25): 17452-17463
57. Kraus E, Janes W, and Barclay A N, The Journal of Immunology, 1998,160:5209-5212
58. Murphy M B, Fuller S T, Richardson P M, Doyle S A. Nucleic Acids Research, 2003, 31(18):e110
59. Li Y, Lee H J, Corn R M. Anal. Chem. 2007,79(3):1082-1088
60. Ho H.-A., Leclerc M. J. Am. Chem. Soc, 2004,126(5): 1384-1387
61. Kirby R, Cho E J, Gehrke B, Bayer T, Park Y S, Neikirk D P, McDevitt J T, Ellington AD. Anal. Chem., 2004, 76(14):4066-4075
62. Pavlov V, Xiao Y, Shlyahovsky B, Willner I. J. Am. Chem. Soc, 2004, 126(38):11768-11769
63. Heckel A, Mayer G J. Am. Chem. Soc, 2005,127(3):822-823
64. Gokulrangan G, Unruh J R, Holub D F, Ingram B, Johnson C K, Wilson G S. Anal. Chem., 2005, 77(7):1963-1970
65. Heyduk E, Heyduk T. Anal. Chem., 2005, 77(4):1147-1156
66. Liss M, Petersen B, Wolf H, Prohaska E. Anal. Chem., 2002, 74(17):448-4495
67. Hianik T, Ostatnav V, Zaijacovaz, Stoikova E, Evtuqvng, Bioorg Med Chem.Lett., 2005,15(2):291-295
68. Bini A, Minunni M, Tombelli S, Centi S, Mascini M. Anal. Chem., 2007, 79(7):3016-3019
69. Liss M, Petersen B, Wolf H, Prohaska E. Anal. Chem., 2002, 74(17):4488-4495
70. Minunni M, Tombelli S, Gullotto A, Luzi E, Mascini M. Biosensors and Bioelectronics, 2004, 20(6):1149-1156
71. Gronewold T M A, Glass S, Quandt E, Famulok M. 2005, 20(10):2044-2052
72. Xu D, Xu D, Yu X, Liu Z, He W, Ma Z. Anal. Chem., 2005, 77(16):5107-5113
73. Radi A.-E., Acero Sanchez J. L., Baldrich E, O'Sullivan C K. Anal. Chem. 2005,77(19):6320-6323
74.Ikebukuro K, Kiyohara C, Sode K. Biosensors and Bioelectronics, 20(10):2168-2172
75. Lai R Y, Plaxco K W, Heeger A J. Anal. Chem. 2007, 79(1):229-233
76. Le Floch F, Ho HA, Leclerc M. 2006, 78(13):4727-4731
77. Hansen J A, Wang J, Kawde A.-N., Xiang Y, Gothelf K V, Collins G J. Am. Chem. Soc, 2006,128(7):2228-2229
78. Zuo X, Song S, Zhang J, Pan D, Wang L, Fan C. J. Am. Chem. Soc, 2007, 129(5):1042-1043
79. Drolet D W, Moon - Mcdermott L , Romig T S. Nature Biotechnology, 196, 14:1021-1025
80. Jhaveri S D, Kirby R, Conrad R, Maglott E J, Bowser M, Kennedy R T, Glick G, Ellington AD. J. Am. Chem. Soc, 2000,122(11):2469-2473
81. Fang X H, Cao Z H, Beck T, Tan W. Anal. Chem., 2001,73(23):5752-5757
82. Yamamoto R., and Kumar P.K.R.. Genes to Cells, 2000,5(5):389-396
83. Su S, Nutiu R, Filipe C D M, Li Y, Pelton R. Langmuir, 2007, 23(3): 1300-1302
84. Stojanovic M N, Landry D W. J. Am. Chem. Soc, 2002,124(33):9678-9679
85. Baker B R, Lai R Y, Wood M S, Doctor E H, Heeger A J, Plaxco K W. J. Am. Chem. Soc, 2006,128(10):3138-3139
86. Uehara M, Barbara B, Dieny B, Stamp P C E. Physics Letters A, 1986, 114(1):23-26
87. Klabunde K J, Stark J, Koper O, Mohs C, Park D G, Decker S, Jiang Y, Lagadic I, Zhang D. J. Phys. Chem., 1996,100(30): 12142-12153
88. Jiang P, Liu Z F, Cai S M. Applied Physics Letters, 1999, 75(19):3023-3025
89. Golabi S M, Nozad A J. Journal of Electroanalytical Chemistry, 2002, 521(1-2):161-167
90. Choi J H, Chen K H, Strano M S. J. Am. Chem. Soc, 2006,128(49):15584-15585
91. Lin Zhangbi ,Su Xingguang ,Zhang Jiahua. Chinese Journal of Analytical Chemistry, 2002, 30(2):237-241.
92. Lin C, Liu Y, Yan H. Nano Lett., 2007, 7(2):507-512
93. Hansen J A, Mukhopadhyay R, Hansen J O, Gothelf K V. 2006, 128(12):3860-3861
94. Daniel M C, Astruc D. Chem. Rev., 2004, 104(1):293-346
95. Turkevitch J, Stevenson P C, Hlillier J. Discuss Faraday Soc, 1951, 11:55-75
96. Frens G. Nature Phys Sci, 1973, 241:20-22
97. Yonezawa T, Kunitake T. Colloids Surf.A: Physicochem. Eng. Asp., 1999, 149:193-199
98. Wang J, Li J H, Baca A J, Hu J, Zhou F, Yan W, Pang D.-W.. Anal. Chem., 2003, 75(15):3941-3945
99. Ray P C, Fortner A, Darbha G K. J. Phys. Chem. B. 2006, 110(42):20745-20748
100. Demers L M, Mirkin C A, Mucic R C, Reynolds R A, Letsinger R L, Elghanian R, Viswanadham G. Anal. Chem., 2000, 72(22):5535-5541
101. Chad A, Letsinger Robert L. Nature, 1996, 382(6592):607-609
102. Storhoff J J, Elghanian R, Mucic R C, Mirkin C A, Letsinger R L. J. Am. Chem. Soc., 1998, 120(9):1959-1964
103. Taton T A, Mucic R C, Mirkin C A, Letsinger R L. J. Am. Chem. Soc., 2000, 122(26):6305-6306
104. Rosi Nathaniel L, Giljohann David A, Thaxton C Shad, Lytton-Jean Abigail K R, Min Su Han, Mirkin Chad A. Science, 2006, 312(19):1027-1030
105. Authier L, Grossiord C, Brossier P, Limoges B. Anal. Chem. 2001. 76(18):4450-4456
106. Wang J, Polsky R, Xu D. Langrnuir, 2001, 17(19):5739-5741
107. Wang J, Xu Danke, Ronen Polsky. J. Am. Chem. Soc., 2002, 124(16):4208-4209
108. Taton T A, Mirkin C A, Letsinger R L. Science, 2000, 289(5485):1757-1760
109.冯琳,宋延林,万梅香,江雷.科学通报,2001,46(16):1321-1324
110.张立德,牟季美:《纳米材料和纳米结构》,北京,科学出版社,2001年
111.邱星屏.厦门大学学报:自然科学版,1999,38(5):711-715
112. Kato K, Radbruch A. Cytometry, 1993, 14(4):384-392
113. Papisov M I, Bogdanov A, Schaffer B, Nossiff N, Shen T, Weissleder R, Brady T J. Journal of Magnetism and Magnetic Materials, 1993, 122(1-3):383-386
114. Ruuge E K, Rusetski A N. Journal of Magnetism and Magnetic Materials, 1993, 122(1-3):335-339
115. Zhu X, Han K, Li G Anal. Chem., 2006, 78(7):2447-2449
116. Katz E, Weizmann Y, Willner I. J. Am. Chem. Soc, 2005,127(25):9191-9200
117. Bruce I J, Sen T. Langmuir, 2005, 21(15):7029-7035
118. Kinsella J M, Ivanisevic A. J. Am. Chem. Soc, 2005,127(10):3276-3277
119. Demirel D, Ozdural A R, Mutlu M. Journal of Food Engineering, 2004, 62(3):203-208
120. David V Morrissey, Massimo Lombardo, John K. Eldredge, Kevin R. Kearney, E. Patrick Groody and Mark L. Collins. Analytical Biochemistry, 1989, 181(2):345-349
121. Shepard AR, Rae J L. Nucleic Acids Research, 1997, 25(15):3183-3185
122. Xu C, Xu K, Gu H, Zhong X, Guo Z, Zheng R, Zhang X, Xu B. J. Am. Chem. Soc, 2004,126(11):3392-3393
123. Doyle P S, Bibette J, Bancaud A, Viovy Jean-Louis. Science, 2002, 295(5563):2237-2238
124. Nam J M, Thaxton C S, Mirkin C A. Science, 2003, 301(5641): 1884-1886
125. Nam J M, Stoeva S I, Mirkin C A. J. Am. Chem. Soc, 2004, 126(19):5932-5933
126. Herr J K, Smith J E, Medley C D, Shangguan D, Tan W. Anal. Chem., 2006, 78(9):2918-2924
127. Dubus S, Gravel J.-E, Le Drogoff B, Nobert P, Veres T, Boudreau D. Anal. Chem., 2006, 78(13):4457-4464
128. Spanova A, Rittich B, Horak Daniel, Lenfeld J, Prod(?)lalova J, Prod(?)lalova J, S trumcova J P S, Journal of Chromatography A, 2003,1009(2):215-221
129. Centi S, Tombelli S, Minunni M, Mascini M. Anal. Chem., 2007, 79(4): 1466-1473
130. Weizmann Y, Elnathan R, Lioubashevski O, Willner I. J. Am. Chem. Soc, 2005, 127(36):12666-12672
131. Josephson L, Perez J M, Weissleder R. Angewandte Chemie International Edition, 2001, 40(17):3204-3206
132. Wang J, Xu D, Polsky R. J. Am. Chem. Soc, 2002, 124(16):4208-4209
133. Bucak S, Jones D A, Laibinis P E, Hatton T A. Biotechnology Progress, 2003, 19(2):477-484
1 Fields S. Science, 2001, 291(5507): 1221-1224
2 Service R F. 2001, 294(5549): 2080-2082
3 Murphy M B, Fuller S T, Richardson P M, Doyle S A. Nucleic Acids Res.,2003, 31(18):1-8
4 Abbott A. Nature, 1999, 402(6763): 715-720
5 Blobel G, Wozniak R W. Nature, 2000, 403(6772): 835-836
6 Ellington A D, Szostak J W.Nature, 1990, 346(467): 818-822
7 Bullock T L, Sherlin L D, Perona J J. Nature structural biology, 2000, 7(6): 497-504
8 Herman T, Patel D J. Science, 2000, 287(5454): 820-825
9 Brody E N, Gold L. Rev. Mol. Biotechnol., 2000, 74(1): 5-13
10 Tombelli S, Minunni M, Mascini M. Biosensors and Bioelectronics, 2005, 20(12): 2424-2434
11 Stojanovic M N, Landry D W. J. Am. Chem. Soc, 2002,124(3): 9678-9679
12 Merino E J, Weeks K M. J. Am. Chem. Soc., 2003, 125(41): 12370-12371
13 Liss M, Petersen B, Wolf H, Prohaska E. Anal. Chem., 2002, 74(17): 4488-4495
14 Xu D, Xu D, Yu X, Liu Z, He W, Ma Z. Anal. Chem., 2005, 77 (16); 5107-5113
15 Kazunori I, Chiharu K, Koji S. Biosensors and Bioelectronics, 2005, 20 (10): 2168-2172
16 Radi A-E, Acero Sanchez J L, Baldrich E. O'Sullivan C K.Anal. Chem., 2005, 77(19): 6320-6323
17 Kawde A N, Rodriguez M C, Lee T M H, Wang J. Electrochem. Commun., 2005, 7(5): 537-540
18 Wang J, Kawde A-N. Magnetic-field stimulated DNA oxidation. Electrochem. Commun. 2002,4 (4):349-352
19 Wang J, Xu D, Polsky R. J.Am.Chem.Soc, 2002, 124(16): 4208-4209
20 Cheng G F, Zhao J, Tu Y H, He P G, Fang Y Z. Analytica Chimica Acta, 2005, 533(1):11-16
21 Maxwell D J, Taylor J R, Nie S. J. Am. Chem. Soc., 2002, 124(32): 9606-9612
22 Mirkin C A, Letsinger R L. Nature, 1996,382 (6592):607-609
23 Cai H, Xu Y, He P G,Fang Y Z. J. Electroanal. Chem., 2001,510(1):78-85
24 Pavlov V, Xiao Y, Shlyahovsky B, Willner I. J. Am. Chem. Soc., 2004, 126(38): 11768-11769
25 Wang X L, Li F, Su Y H, Sun X, Li X B, Schluesener H J, Tang F, Xu S Q. Anal. Chem, 2004, 76(19): 5605-5610
26 Pumera, M, Castaneda M T, Pividori M I, Eritja R, Merkoci A, Alegret S. Langmuir, 2005, 21(21):9625-9629
27 Doron A, Katz E, Willner I. Langmuir, 1995,11(4):1313-1317
28 Ho H-A, Leclerc M. J. Am. Chem. Soc., 2004, 126(5): 1384-1387
29 Heyduk E, Heyduk T. Anal. Chem., 2005, 77(4): 1147-1156
30 徒永华,程桂芳,林莉,郑静,何品刚,方禹之..高等学校化学学报,2006,27(12):2266-2270
[1] Fields S. Science[J], 2001, 291(5507): 1221—1223
[2] Ellington A. D., Szostak J. W. Nature[J], 1990, 346(6287): 818—822
[3] Bock L. C., Griffin L. C. Nature[J], 1992, 355(6360): 564—566
[4] Tombelli S., Minunni M., Mascini M.. Biosens. Bioelectron.[J], 2005, 20(15): 2424—2434
[5] German I., Buchanan D. D., KennedY R. T. Anal. Chem.[J], 1998, 70(21): 4540—4545
[6] Fang X., Cao Z., Beck T., et al.. Anal. Chem.[J], 2001, 73(23): 5752—5757
[7] Heyduk E., Heyduk T.. Anal. Chem.[J], 2005, 77(4): 1147—1156
[8] Wang J., Xu D., Kawade A.-N., et al.. Anal. Chem.[J], 2001, 73(22): 5576—5581
[9] Park S.-J., Talon T. A., Mirkin C. A.. Science[J], 2002, 295(5559):1503—1506
[10] Zheng Jing(郑静), Cheng Gui-Fang(程圭芳), Wang An-Bao(王安宝), et al.. Science in China serial B (中国科学B辑) [J], 2006, 36(6): 485—492
[11] Cai H., Xu Y., He P. G., et al.. J. Electroanal. Chem.[J], 2001, 510(1): 78—85
[12] Wang J., Kawade A. N.. Electrochemistry Communications[J], 2002, 4(4): 349—352
[13] Wang J., Xu D., Polsky R. J. Am. Chem. Soc.[J], 2002, 124(16): 4208—4209
[14] Cheng G. E, Zhao J., Tu Y. H., et al.. Analytica Chimica Acta[J], 2005, 533(1):11—16
[15] Richardson J., Hawkins P., Luxton R.. Biosensors and Bioelectronics[J], 2001, 16(11): 989—993
[16] Pavlov V., Xiao Y., Shlyahovsky B., et al.. J. Am. Chem. Soc.[J], 2004, 126(38): 11768—11769
[17] Bock L. C., Griffin L. C.. Nature[J], 1992, 355(6360): 564—566
[18] Tasset D. M., Kubik M. F., Steiner W.. Journal of Molecular Biology[J], 1997, 272(5): 688—698
[19] Han M., Lytton-Jean A. K. R., Oh B.-K., et.al. Angew. Chem.[J], 2006, 118(11): 1839—1842
[20] Jiang Y., Fang X., Bai C.. Anal. Chem.[J], 2004, 76(17): 5230—5235
[21] Gokulrangan G., Unrnh J. R., Holub D. F., et al.. Anal. Chem.[J], 2005, 77(7): 1963—1970.
[22] Amihood D., Eugeni K., Itamar W.. Langmuir[J], 1995, 11(4): 1313—1317
[23] Elghanian R., Sorhoff J.J. Science[J], 1997, 277(5329): 1078—1081
[24] Tu Yong-Hua(徒永华), Cheng Gui-Fang(程圭芳), Lin Li(林莉), et al.. Chem. J. Chinese Universities (高等学校化学学报) [J], 2006, 27(12): 2266—2270
[25] Mucic R. C., Storhoff J. J., Mirkin C. A., et al.. J. Am. Chem. Soc.[J], 1998, 120(48): 12674—12675
[26] Demers L. M., Mirkin C. A., Mucic R. C., et al.. Anal. Chem.[J], 2000, 72(22): 5535—5541
[27] Hamaguchin N., Ellington A., Stanton M.. Analytical Biochemistry[J], 2001, 294(2): 126—131
[28] Nutiu R., Li Y.. J. Am. Chem. Soc.[J], 2003, 125(16): 4771—4778
[29] Wendy U. D., Andreas R., Friedrich C. S.. Angewandte Chemie International Edition[J], 2004, 43(27): 3550—3553
1. Fields, S., 2001.Science 291,1221-1224.
2. Merkoci, A., Aldavert, M., Tarrason, G., Eritja, R., Alegret, S., 2005. Aanlytical Chemistry 77,6500-6503.
3. Ellington, A.D., Szostak, J.W., 1990. Nature 346, 818-822.
4. Tuerk, C., Goldberg, L., 1990. Science 249, 505-510.
5. Robertson, D. L., Joyce, G.F., 1990. Nature 344, 467-468.
6. Bock, L.C., Griffin, L.C., 1992. Nature 355, 564-567.
7. German, I., Buchanan, D.D., Kennedy, R.T., 1998. Anal. Chem. 70, 4540-4545.
8. Stojanovic, M.N., Landry, D.W., 2002. J. Am. Chem. Soc. 124, 9678-9679.
9. Merino, E.J., Weeks, K.M., 2003. J. Am. Chem. Soc. 125, 12370-12371.
10. Liss, M., Petersen, B., Wolf, H., Prohaska, E., 2002. Anal. Chem. 74, 4488-4495.
11. Kawde, A.N., Rodriguez, M.C., Lee, T.M.H., Wang, J., 2005, Electrochemistry Communications 7, 537-540.
12. Wang, J., Xu, D., Kawde, A.N., Polsky, R., 2001. Anal. Chem. 73, 5576-5581.
13. Park, S.J., Taton, T.A., Mirkin C.A., 2002. Science. 295,1503-1507
14. Wang, J., Kawde, A.-N., 2002. Electrochemistry Communications 4, 349-352.
15. Wang, J., Xu, D., Polsky, R., 2002. J. Am. Chem. Soc. 124, 4208-4209.
16. Cheng, G.F., Zhao, J., Tu, Y.H., He, P.G., Fang, Y.Z., 2005. Analytica Chimica Acta 533, 11-16.
17. Laios, E., Loannou, P.C., Christopoulos, T.K., 2001. Anal. Chem. 73, 689-692.
18. Zhang, Y., Kim, H.-H., Heller, A., 2003. Anal. Chem. 75, 3267-3269.
19. Yin, X.-B., Qi, B., Sun, X., Yang, X., Wang, E., 2005. Anal. Chem. 77, 3525-3530.
20. Soto, C.M., Blum, A.S., Vora, G.J., Lebedev, N., Meador, C.E., Won, A.P., Chatterji, A., Johnson, J. E., Ratna, B.R., 2006. J. Am. Chem. Soc. 128, 5184-5189.
21. Maxwell, D.J., Taylor, J.R., Nie, S., 2002. J. Am. Chem. Soc. 124, 9606-9612.
22. Mirkin, C. A., Letsinger, R. L.,1996 .Nature 382,607-609.
23. Taton, T.A., Mirkin, C.A., Letsinger, R.L., 2000. Science 289,1757-1760.
24. Pumera, M., Castaneda, M.T., Pividori, M.I., Eritja, R., Merkoci, A., Alegret, S., 2005. Langmuir 21,9625-9629.
25. Zheng, J., Lin, L., Cheng, G.F., Wang, A.B., Tan, X.L., He, P.G., Fang,Y.Z., 2006.Science in China, serial B 36, 485-492.
26. Pavlov, V., Xiao, Y., Shlyahovsky, B., Willner, I., 2004. Am.Chem.Soc. 126, 11768-11769.
27. Tan, Y.W., Li, Y.F., Zhu, D.F., 2002. Langmuir 18, 3392-3395.
28. Li, J.J., Fang, X.H., Jiang, Y., Fang, X., Bai, C, 2004. Anal. Chem. 76, 5230-5232.
29. Gokulrangan, G., Unruh, J.R., Holub, D.F., Ingram, B., Johnson, C.K., Wilson, G.S., 2005. Anal. Chem. 77, 1963-1970.
30. Amihood, D., Eugenii, K., Itamar, W., 1995. Langmuir 11,1313-1317.
31. Ho, H.-A., Leclerc, M., 2004. J. Am. Chem. Soc. 126,1384-1387.
32. Huang, C.-C, Huang, Y.-F., Cao, Z., Tan, W., Chang, H.-T., 2005. Anal. Chem. 77, 5735-5741.
33. Radi, A.-E., Acero Sanchez, J.L., Baldrich, E., O'Sullivan, C.K., 2006. J. Am. Chem. Soc. 128,117-124.
34. Polsky, R., Gill, R., Kaganovsky, L., Willner, I., 2006. Anal. Chem. 78, 2268-2271.
35. Wang, X.-L., Li, F., Su, Y.-H., Sun, X., Li, X.-B., Schluesener, H.J., Tang, F., Xu, S.-O., 2004. Anal. Chem. 76, 5605-5610.
36. Le Floch, F., Ho, H.A., Leclerc, M., 2006. Anal. Chem. 78, 4727-4731.
37. Heyduk, E., Heyduk, T., 2005. Anal. Chem. 77, 1147-1156.
38. Esmon, C.T., Jacksons, C.M., 1974. Biological Chemistry 249, 7791-7797.
39. Gu, H.Z., 1999. Journal of Mathematical Medicine 12, 285-286.
40. Ferguson M.D., J.H., Travis B.A., B.L., Gerheim M.S., E.B., 1948. Blood 3, 1130-1160.
1.罗春雄,毛有东,欧阳颀.生物物理学报,2005,21(2):151-156
2. Ryan, K. M.; Vousden, K. H. Nature 2002, 419(6909): 795-797.
3.张丽华,侯振江.临床肺科杂志,2006,11(1):59-60
4. Partridge M, Li S R, Pateromichelaki S, Francis R A, Philips E, Huang X.-H.; Tesfa-Selase F, Langdon J D. Clin.Cancer Res., 2000, 6(7): 2718-2725
5. Hollstein M, Sidranskv D, Vogelstein B. Science, 1991, 253 (5015):49-53
6. Tachibana, M.; Shinagawa, Y.; Kawamata, H.; Omotehara, F.; Horiuchi, H.; Ohkura, Y. Anticancer Res. 2003, 23(3),2891-2896
7. Miyajima K, Tamiya S, Oda Y, Adachi T, Konomoto T, Toyoshiba H, Masuda K, Tsuneyoshi M. Cancer Lett, 2002, 164(2):177-188
8.蔡文琴:《现代实用细胞与分子生物学实验技术》,北京,人民军医出版社, 2003年
9. Marquette C A, Lawrence M F, Blum L J. Anal. Chem, 2006, 78(3):959-964
10.Tan Y, Li Y, Zhu D. Langmuir, 2002,18(8):3392-3395
11.Taton T Andrew, Mirkin Chad A, Letsinger Robert L. Science, 2000, 289(5486):1757-1760
12.Duan X, Li Z, He F, Wang S. J. Am. Chem. Soc, 2007,129(14):4154-4155
1.黄松音,段朝晖,梁穆兴,林向华,范侠.血栓与止血学,2002,8(4):156-156
2. Herman R. P. ThrombHaemost [J],1979,41:544-547;
3.张莹,魏文宁.微循环学杂志,2005,15(2):70-72
4. Yan Hui-Min, Huang Ji-Qun, Liao Zhao-Quan. Academic Journal of Guangzhou Medical College(广州医学院学报), 1995, 23(5):33-37
5. Bianchini Michele, Radrizzani Martin, Brocardo M G, Reyes G B, Solveyra C G, Santa-Coloma TA. J. Immunol. Methods, 2001, 252(1):191-197
6. Murphy M B, Fuller S T, Richardson P M, Doyle S A. Nucleic Acids Research, 2003, 31(18):e110
7. Ellington A D, Szostak J W. Nature, 1990, 346:818-822
8. Ikebukuro Kazunori, Kiyohara Chiharu, Sode Koji. Biosensors and Bioelectronics, 2005, 20(10):2168-2172
9. Hesselberth J, Robertson M P, Jhaveri S, Ellington A D. Reviews in Molecular Biotechnology, 2000, 74(1):15-25
10. Kirkham P M, Neri D, Winter G. Journal of Molecular Biology, 1999, 285(3):909-915
11. Hamaguchi Nobuko, Ellington Andrew, Stanton Martin. Analytical Biochemistry, 2001, 294(2):126-131
12. Nutiu Razvan, Li Yingfu. J. Am. Chem. Soc, 2003, 125(16):4771-4778
13. Dittmer W U, Reuter A, Simmel F C. Angewandte Chemie International Edition, 2004, 43(27):3550-3553
14. Xiao Y, Piorek B D, Plaxco K W, Heeger A J. J. Am. Chem. Soc, 2005, 127(51):17990-17991
15. Rupcich N, Nutiu R, Li Y, Brennan J D. Anal. Chem., 2005, 77(14):4300-4307
16. Nutiu R, Li Y. J. Am. Chem. Soc., 2003, 125(16):4771-4778
17. Elghanian Robert, Storhoff James J. Science, 1997, 277(5329): 1078-1081
18. Taton TA, Mirkin C A, Letsinger R L. Science, 2000, 289(5485):1757-1760
19.许善峰,李芳,姜涌明,杨生妹.中国医学生物技术应用杂志,2004,3(3):18-23
20. Cera E Di, Dang Q D, Ayala Y M. Cellular and Molecular Life Sciences, 1997, 53(9):701-730
21. Ho H A, Leclerc M. J. Am. Chem. Soc, 2004,126(5): 1384-1387
22. Lee M, Walt D R, Anal. Biochem., 2000, 282(1):142-146
23. Jiang Y, Fang X, Bai C. 2004, 76(17):5230-5235
24. V. A. Spiridonova, E. V. Rog, T. N. Dugina, S. M. Strukova, A. M. Kopylov. Russian Journal of Bioorganic Chemistry, 2003, 29(5):450-453
25. Kneuer Carsten, Sameti Mohammad, Bakowsky Udo, Schiestel Thomas, Schirra Hermann, Schmidt Helmut, Lehr Claus-Michael. Bioconjugate Chem., 2000, 11(6):926-932
1. Ellington A D, Szostak J W. Nature, 1990, 346(6287):818-822
2. Bock L. C., Griffin L. C.. Nature[J], 1992, 355(6360): 564—566
3. German I., Buchanan D. D., Kennedy R. T.. Anal. Chem.[J], 1998, 70(21): 4540—4545
4. Bullock T L, Sherlin L D, Perona. Nature structural biology, 2000, 7(6):497-504
5. Hermann T, Patel D J. Science, 2000, 287(5454):820-825
6. Tucker C E, Chen L, Judkins M B, Farmer J A, Gill S C, Drolet D W. Journal of Chromatography B: Biomedical Sciences and Applications, 1999, 732(1):203-212
7. Drolet D W, Nelson J, Tucker C E, Zack P M, Nixon Kerry, Bolin Richard, Judkins M B, Farmer J A, Wolf J L, Gill S C, Bendele R A. Pharmaceutical Research, 2000, 17(12): 1503-1510
8. Blank M, Weinscbenk T, Prijemer M, Schluesener H. Journal of Biological Chemistry, 2001, 276(19):16464-16468
9. Ostendore Tammo, Kunter Uta, Grone Hermann Joseph, Bahlmann Ferdinand, Kawachi Hirroshi, Shimizu Fujio, Koch Karl Martin, Janjic Nebojsa, Floege Jurgen. Journal of the American Society of Nephrology, 2001, 12(5):909-918
10. Berezovski M, Krylov S N. Anal. Chem., 2005, 77(5):1526-1529
11. Olsen C M, Gmeiner W H, Marky L A. J. Phys. Chem. B., 2006, 110(3):6962-6969
12. Hamaguchi N, Ellington A D, Stanton M. 2001, 294(2):126-131
13. Nutiu R, Li Y. J. Am. Chem. Soc., 2003, 125(6):4771-4778
14. Dittmer W U, Reuter A, Simmel F C. Angewandte Chemie International Edition, 2004, 43(27):3550-3553
15. Xiao Y, Piorek B D, Plaxco K W, Heeger A J. J. Am. Chem. Soc., 2005, 127(51):17990-17991
16. Rupcich N, Nutiu R, Li Y, Brennan J D. Anal. Chem., 2005, 77(14):4300-4307
17. Mbebi Corinne, Rohn Troy, Doyennette Marie-Agnes, Chevessier Frederic, Jandrot-Perrus Martine, Hantai Daniel, Verdiere-Sahuque Martine. Experimental Cell Research, 2001, 263(1):77-87
18. Bock L C, Griffin L C, Latham J A, Vermass E H, Toole J J. Nature, 1992, 355(6360):564-566
19. Tasset D M, Kubik M F, Steiner W. Journal of Molecular Biology, 1997, 272(5):688-698
20. Wang X L, Li F, Su Y H, Sun X, Li X B, Schluesener H J, Tang F, Xu S Q. Anal. Chem., 2004, 76(19):5605-5610
21.周爱儒,生物化学,第六版,人民卫生出版社,2005,8
22.聂剑初,吴国利,张翼伸,杨绍钟,刘鸿铭.合编 生物化学简明教程,高等学校出版社,第三版,2005.11
23. Hermann Thomas, Patel Dinshaw J. Science, 2000, 287(5454):820-825
24. Spiridonova V A, Rog E V, Dugina T N, Strukova S M, Kopylov A M. Russian Journal of Bioorganic Chemistry, 2003, 29(5):450-453
25. Ho H A, Leclerc M. J. Am. Chem. Soc., 2004, 126(5):1384-1387