草坪草种子超干贮藏及PEG引发方法的研究
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摘要
本试验选用高羊茅(美洲虎 3 号(1)、美洲虎 3 号(2)、全体、爱瑞 3 号)、多年生黑麦草(爱神特)、草地早熟禾(公园)、普通狗牙根(脱壳和未脱壳两种)等 8种草坪草种子为材料,进行超干贮藏和种子引发研究,通过分析贮藏前后草坪草种子各种活力指标的变化,探讨不同类型及品种草坪草种子常温贮藏和低温贮藏适宜的低水分值或超低水分值;研究 PEG 引发对草坪草种子萌发及活力的影响,探讨草坪草种子 PEG 引发的适宜方法。
1.采用变色硅胶作干燥剂对草坪草种子进行吸湿干燥,获得 8 种草坪草种子不同档次的水分,每个水分档次取两份材料分别置于常温和低温(0~ -5℃)下密封贮藏 6 个月。
对贮藏前后的草坪草种子分别进行幼苗生长测定、加速老化试验、低温处理试验、电导率测定、模拟田间出苗试验,过氧化物酶、过氧化氢酶、超氧化物歧化酶、脱氢酶和酸性磷酸酶的活性测定以及丙二醛含量测定,比较贮藏前后种子各种活力指标的差异,结果表明,各种草坪草种子均可寻找出常温贮藏和低温贮藏的适宜低水分值或超低水分值,且品种间存在差异。
根据本试验结果,初步认为:美洲虎 3 号(1)常温贮藏适宜的低水分和超低水分分别为 8.86%和 4.66%,低温贮藏适宜的低水分和超低水分分别为 8.86%和 3.56%;美洲虎 3 号(2)常温和低温贮藏适宜的低水分和超低水分均相同,分别为 8.96%和4.94%;全体常温贮藏适宜的超低水分为 4.64%,低温贮藏适宜的低水分为 9.53%;爱瑞 3 号常温贮藏适宜的低水分为 8.38%,低温贮藏适宜的超低水分为 3.74%;爱神特常温贮藏适宜的低水分和超低水分分别为 8.51%和 4.98%,低温贮藏适宜的超低水分为 4.98%;公园常温和低温贮藏适宜的超低水分均为 3.38%;未脱壳狗牙根常温贮藏适宜的超低水分为 4.94%,低温贮藏适宜的低水分和超低水分分别为 7.87%和3.52%;脱壳狗牙根常温贮藏适宜的低水分和超低水分分别为 6.97%和 3.87%,低温贮藏适宜的超低水分为 3.87%。
2.相关分析表明,幼苗生长测定、加速老化试验、低温处理试验所测得的各项活力指标除个别指标与模拟田间出苗率相关不显著外,其他如发芽势、芽长、芽鲜重、
Eight Turfgrass Seeds were used as materials in the experiment, which were Festuca ovina (Jaguar 3 (1)、Jaguar 3 (2)、Total and Arid 3)、Lolium perenne (Accent)、Poa pratensis (Park)and Cynodon dactylon (Unhulled and Hulled).They were tested by ways of ultra-dry storage and seed priming. The changes of different vigor indexes of Turfgrass seeds were analyzed before and after storage, and Suitable ultra-low moisture value and low moisture value of different Turfgrass seeds were found. Through studying the effect of PEG priming on germination energy and vigor of Turfgrass seeds, suitable methods of PEG priming were found.
1.Turfgrass seeds were dried by discolored silica gel as drier, and different moisture grades of eight Turfgrass seeds were got. Two materials of each moisture grade were fetched, put, sealed and preserved for six months under normal temperature and low temperature (0~-5℃) separately.
Turfgrass seeds before and after storage were tested by ways of seedling growth test, accelerated ageing test, cold test, electrical conductivity test, simulated field test, activation test of POD, CAT,SOD,TTC and APA and MDA content test. Compared the difference of different vigor indexes before and after storage, the results showed that suitable low moisture value or ultra-low moisture value of eight Turfgrass seeds were found in normal temperature storage and low temperature storage, and there were differences among the varieties.
On the basis of the experiment,the preliminary results were as follows: Suitable low moisture and ultra-low moisture of Jaguar 3 (1) under normal temperature storage were separately 8.86% and 4.66%, and suitable low moisture and ultra-low moisture of low temperature storage were separately 8.86% and 3.56%; Suitable low moisture and ultra-low moisture of Jaguar 3 (2) under normal temperature storage and low temperature storage were the same, and they were 8.96% and 4.94% separately; Suitable ultra-low moisture of normal temperature storage of Total was 4.64%,and suitable low moisture of low temperature storage was 9.53%; Suitable low moisture of normal temperature storage of Arid 3 was 8.38%,and suitable ultra-low moisture of low temperature storage was 3.74%; Suitable low moisture and ultra-low moisture of Accent under normal temperature storage were separately 8.51% and 4.98%,
and suitable ultra-low moisture of low temperature storage was 4.98%; Suitable ultra-low moisture of Park under normal temperature storage and low temperature storage was 3.38%; Suitable ultra-low moisture of normal temperature storage of Unhulled was 4.94%, and suitable low moisture and ultra-low moisture of low temperature storage were separately 7.87% and 3.52%; Suitable low moisture and ultra-low moisture of Hulled under normal temperature storage were separately 6.97% and 3.87%, and suitable ultra-low moisture of low temperature storage was 3.87%.
2.The result of regression analysis showed that through seedling growth test, accelerated ageing test and cold test, some of vigor indexes were not significantly correlated with seedling rate of simulated field condition, and other were highly significantly correlated positively with seedling rate of simulated field condition such as germination energy, sprout length, sprout fresh weight, sprout dry weight and simple vigor index. These showed that seedling growth test, accelerated ageing test and cold test were all better test methods for vigor test of Turfgrass seeds.
Both electrical conductivity and absolute conductivity were highly significantly correlated positively with seedling rate of simulated field condition, and relative conductivity was significantly correlated positively with seedling rate of simulated field condition.
The activations of POD, CAT, TTC and APA were highly significantly correlated positively with seedling rate of simulated field condition, while SOD activation was not significantly correlated with seedling rate of simulated field condition, and MDA content was significantly correlated positively with seedling rate of simulated field
1.采用变色硅胶作干燥剂对草坪草种子进行吸湿干燥,获得 8 种草坪草种子不同档次的水分,每个水分档次取两份材料分别置于常温和低温(0~ -5℃)下密封贮藏 6 个月。
对贮藏前后的草坪草种子分别进行幼苗生长测定、加速老化试验、低温处理试验、电导率测定、模拟田间出苗试验,过氧化物酶、过氧化氢酶、超氧化物歧化酶、脱氢酶和酸性磷酸酶的活性测定以及丙二醛含量测定,比较贮藏前后种子各种活力指标的差异,结果表明,各种草坪草种子均可寻找出常温贮藏和低温贮藏的适宜低水分值或超低水分值,且品种间存在差异。
根据本试验结果,初步认为:美洲虎 3 号(1)常温贮藏适宜的低水分和超低水分分别为 8.86%和 4.66%,低温贮藏适宜的低水分和超低水分分别为 8.86%和 3.56%;美洲虎 3 号(2)常温和低温贮藏适宜的低水分和超低水分均相同,分别为 8.96%和4.94%;全体常温贮藏适宜的超低水分为 4.64%,低温贮藏适宜的低水分为 9.53%;爱瑞 3 号常温贮藏适宜的低水分为 8.38%,低温贮藏适宜的超低水分为 3.74%;爱神特常温贮藏适宜的低水分和超低水分分别为 8.51%和 4.98%,低温贮藏适宜的超低水分为 4.98%;公园常温和低温贮藏适宜的超低水分均为 3.38%;未脱壳狗牙根常温贮藏适宜的超低水分为 4.94%,低温贮藏适宜的低水分和超低水分分别为 7.87%和3.52%;脱壳狗牙根常温贮藏适宜的低水分和超低水分分别为 6.97%和 3.87%,低温贮藏适宜的超低水分为 3.87%。
2.相关分析表明,幼苗生长测定、加速老化试验、低温处理试验所测得的各项活力指标除个别指标与模拟田间出苗率相关不显著外,其他如发芽势、芽长、芽鲜重、
Eight Turfgrass Seeds were used as materials in the experiment, which were Festuca ovina (Jaguar 3 (1)、Jaguar 3 (2)、Total and Arid 3)、Lolium perenne (Accent)、Poa pratensis (Park)and Cynodon dactylon (Unhulled and Hulled).They were tested by ways of ultra-dry storage and seed priming. The changes of different vigor indexes of Turfgrass seeds were analyzed before and after storage, and Suitable ultra-low moisture value and low moisture value of different Turfgrass seeds were found. Through studying the effect of PEG priming on germination energy and vigor of Turfgrass seeds, suitable methods of PEG priming were found.
1.Turfgrass seeds were dried by discolored silica gel as drier, and different moisture grades of eight Turfgrass seeds were got. Two materials of each moisture grade were fetched, put, sealed and preserved for six months under normal temperature and low temperature (0~-5℃) separately.
Turfgrass seeds before and after storage were tested by ways of seedling growth test, accelerated ageing test, cold test, electrical conductivity test, simulated field test, activation test of POD, CAT,SOD,TTC and APA and MDA content test. Compared the difference of different vigor indexes before and after storage, the results showed that suitable low moisture value or ultra-low moisture value of eight Turfgrass seeds were found in normal temperature storage and low temperature storage, and there were differences among the varieties.
On the basis of the experiment,the preliminary results were as follows: Suitable low moisture and ultra-low moisture of Jaguar 3 (1) under normal temperature storage were separately 8.86% and 4.66%, and suitable low moisture and ultra-low moisture of low temperature storage were separately 8.86% and 3.56%; Suitable low moisture and ultra-low moisture of Jaguar 3 (2) under normal temperature storage and low temperature storage were the same, and they were 8.96% and 4.94% separately; Suitable ultra-low moisture of normal temperature storage of Total was 4.64%,and suitable low moisture of low temperature storage was 9.53%; Suitable low moisture of normal temperature storage of Arid 3 was 8.38%,and suitable ultra-low moisture of low temperature storage was 3.74%; Suitable low moisture and ultra-low moisture of Accent under normal temperature storage were separately 8.51% and 4.98%,
and suitable ultra-low moisture of low temperature storage was 4.98%; Suitable ultra-low moisture of Park under normal temperature storage and low temperature storage was 3.38%; Suitable ultra-low moisture of normal temperature storage of Unhulled was 4.94%, and suitable low moisture and ultra-low moisture of low temperature storage were separately 7.87% and 3.52%; Suitable low moisture and ultra-low moisture of Hulled under normal temperature storage were separately 6.97% and 3.87%, and suitable ultra-low moisture of low temperature storage was 3.87%.
2.The result of regression analysis showed that through seedling growth test, accelerated ageing test and cold test, some of vigor indexes were not significantly correlated with seedling rate of simulated field condition, and other were highly significantly correlated positively with seedling rate of simulated field condition such as germination energy, sprout length, sprout fresh weight, sprout dry weight and simple vigor index. These showed that seedling growth test, accelerated ageing test and cold test were all better test methods for vigor test of Turfgrass seeds.
Both electrical conductivity and absolute conductivity were highly significantly correlated positively with seedling rate of simulated field condition, and relative conductivity was significantly correlated positively with seedling rate of simulated field condition.
The activations of POD, CAT, TTC and APA were highly significantly correlated positively with seedling rate of simulated field condition, while SOD activation was not significantly correlated with seedling rate of simulated field condition, and MDA content was significantly correlated positively with seedling rate of simulated field
引文
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