史氏鲟、西伯利亚鲟及其杂交种生化遗传研究
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摘要
鲟鱼是优质名贵鱼种,具有重要的科研经济价值。本研究通过同工酶分析方法系统研究史氏鲟、西伯利亚鲟、史氏鲟(♀)×西伯利亚鲟(♂)杂交种及史氏鲟(♂)×西伯利亚鲟(♀)杂交种4个鲟鱼群体的遗传多样性,为鲟鱼种质保护、资源开发利用及良种选育与推广提供科学依据。
史氏鲟、西伯利亚鲟、史氏鲟(♀)×西伯利亚鲟(♂)杂交种及史氏鲟(♂)×西伯利亚鲟(♀)杂交种的同工酶分析结果表明:
1.4个鲟鱼群体的心、肝、肌、眼、肾5种组织间的同工酶表达呈现明显的特异性,其中以EST、α-Amy、ADH、GDH、MDH较为显著。
2.通过4个群体5种组织9种同工酶比较分析发现,杂交种的酶带表达比亲本复杂,但多与母本酶带表达相近。
3.群体遗传多样性分析表明,4个鲟鱼群体的多态座位比例差异较大,平均值为30.00%,平均每个位点的有效等位基因数为1.3130,平均杂合度预期值为0.1637,各群体的Shannon多样性指数平均为0.2320。Hardy-Weinberg遗传平衡符合度检验表明4个鲟鱼群体遗传变异性比较高,处于杂合子过剩状态。
4.聚类分析表明,史氏鲟、史氏鲟(♀)×西伯利亚鲟(♂)杂交种聚为一个分支,西伯利亚鲟、史氏鲟(♂)×西伯利亚鲟(♀)杂交种聚为另一分支,杂交种的遗传信息表达多与母本接近。
Acipenser is a high quality valuable fish.Study on the genetic diversity of Acipenser schrenckii、Acipenser baeri、Acipenser schrenckii(♀)×Acipenser baeri(♂)、Acipenser schrenckii(♂)×Acipenser baeri(♀)using allozyme analyses,has important content,at the same time,protects fish genetic diversity and germ plasm resources,and promotes sustainable utility of bioresources.
Biochemical genetic differences of Acipenser schrenckii、Acipenser schrenckii(♀)×Acipenser baeri(♂)、Acipenser baeri、Acipenser schrenckii(♂)×Acipenser baeri(♀)were analyzed by polyacrylamide gel electrophoresis.The results indicated:
1.the expression of all the isozymes studied was highly tissue-specific.Especially among EST,α-Amy,ADH,GDH,MDH were more apparently.
2.Acipenser schrenckii、Acipenser schrenckii(♀)×Acipenser baeri(♂)、Acipenser schrenckii(♂)×Acipenser baeri(♀)、Acipenser baeriwere analyzed by polyacrylamide gel electrophoresis.The results indicated:Acipenser schrenckii(♀)×Acipenser baeri(♂)、Acipenser schrenckii(♂)×Acipenser baeri(♀)showed great difference between Acipenser schrenckii、Acipenser baeri.But close to female parents.
3.The percentage of polymorphic loci had small difference among populations,mean value was 30.00%,the average effective number of alleles was 1.3130,the Nei's expected heterozygosity was0.1637,and Shannon's Information index was 0.2320.Hardy-Weinberg heredity equilibrium proof-test indicated that Hemibarbus labeo resource quality is good.
4.Phylogenetic tree showed Acipenser schrenckii、Acipenser schrenckii(♀)×Acipenser baeri(♂)belong to the same clade,and Acipenser baeri、Acipenser schrenckii{♂)×Acipenser baeri(♀)belong to the same clade.Genetic diversity of their reciprocal close to female parents.
史氏鲟、西伯利亚鲟、史氏鲟(♀)×西伯利亚鲟(♂)杂交种及史氏鲟(♂)×西伯利亚鲟(♀)杂交种的同工酶分析结果表明:
1.4个鲟鱼群体的心、肝、肌、眼、肾5种组织间的同工酶表达呈现明显的特异性,其中以EST、α-Amy、ADH、GDH、MDH较为显著。
2.通过4个群体5种组织9种同工酶比较分析发现,杂交种的酶带表达比亲本复杂,但多与母本酶带表达相近。
3.群体遗传多样性分析表明,4个鲟鱼群体的多态座位比例差异较大,平均值为30.00%,平均每个位点的有效等位基因数为1.3130,平均杂合度预期值为0.1637,各群体的Shannon多样性指数平均为0.2320。Hardy-Weinberg遗传平衡符合度检验表明4个鲟鱼群体遗传变异性比较高,处于杂合子过剩状态。
4.聚类分析表明,史氏鲟、史氏鲟(♀)×西伯利亚鲟(♂)杂交种聚为一个分支,西伯利亚鲟、史氏鲟(♂)×西伯利亚鲟(♀)杂交种聚为另一分支,杂交种的遗传信息表达多与母本接近。
Acipenser is a high quality valuable fish.Study on the genetic diversity of Acipenser schrenckii、Acipenser baeri、Acipenser schrenckii(♀)×Acipenser baeri(♂)、Acipenser schrenckii(♂)×Acipenser baeri(♀)using allozyme analyses,has important content,at the same time,protects fish genetic diversity and germ plasm resources,and promotes sustainable utility of bioresources.
Biochemical genetic differences of Acipenser schrenckii、Acipenser schrenckii(♀)×Acipenser baeri(♂)、Acipenser baeri、Acipenser schrenckii(♂)×Acipenser baeri(♀)were analyzed by polyacrylamide gel electrophoresis.The results indicated:
1.the expression of all the isozymes studied was highly tissue-specific.Especially among EST,α-Amy,ADH,GDH,MDH were more apparently.
2.Acipenser schrenckii、Acipenser schrenckii(♀)×Acipenser baeri(♂)、Acipenser schrenckii(♂)×Acipenser baeri(♀)、Acipenser baeriwere analyzed by polyacrylamide gel electrophoresis.The results indicated:Acipenser schrenckii(♀)×Acipenser baeri(♂)、Acipenser schrenckii(♂)×Acipenser baeri(♀)showed great difference between Acipenser schrenckii、Acipenser baeri.But close to female parents.
3.The percentage of polymorphic loci had small difference among populations,mean value was 30.00%,the average effective number of alleles was 1.3130,the Nei's expected heterozygosity was0.1637,and Shannon's Information index was 0.2320.Hardy-Weinberg heredity equilibrium proof-test indicated that Hemibarbus labeo resource quality is good.
4.Phylogenetic tree showed Acipenser schrenckii、Acipenser schrenckii(♀)×Acipenser baeri(♂)belong to the same clade,and Acipenser baeri、Acipenser schrenckii{♂)×Acipenser baeri(♀)belong to the same clade.Genetic diversity of their reciprocal close to female parents.
引文
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[13]李国庆,伍育源,秦志峰等,鱼类遗传多样性研究.水产科学,2004,23(8):42-44
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[17]W.C.Jordan,T.F.Cross,W.W.Crozier.Allozyme variation in Atlantic salmon from the British Isles:associations with geography and the environment.Journal of Fish Biology,2005,67(Supplement A),146-168
[18]王成辉,李思发,赵金良.我国4种红鲤群体的生化遗传差异.上海水产大学学报,2004,13(1):1-4
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[21]曾伯平,陈昌福,纪国良.草鱼病理学研究.中山大学学报,1995,34(1):76-81
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