针刺对糖尿病心肌病大鼠模型TNF-α调节作用的实验研究
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摘要
目的:通过针刺方法治疗实验性糖尿病心肌病大鼠,观察其一般状态、血糖、TNF-α的变化,观察心肌超微结构的改变,探讨TNF-α在糖尿病心肌病发生、发展中的作用,旨在进一步分析发病机理及针刺治疗的原理,为针灸治疗糖尿病心肌病提供现代科学依据。
方法:1.将符合要求的雄性Wistar大鼠58只,随机分为正常对照组8只,其余50只单次腹腔注射链脲佐菌素55mg/kg制造糖尿病心肌病大鼠模型,造模成功42只,随机分为模型组14只、针刺治疗组14只、依那普利治疗组14只。2.针刺治疗组选肺俞、脾俞、肾俞、内关、三阴交、胃脘下俞,每日1次针刺,每次15min,平补平泻,连续针刺12周;依那普利治疗组每日上午根据大鼠体重灌服依那普利1.5mg/kg;正常对照组、模型组每日上午灌服等体积饮用水。3.采用放射免疫分析法(RIA),测定TNF-α含量;应用常规生化指标检测血糖;透射电镜下观察心肌超微结构并拍照;测定大鼠14周末体重进行对比。
结果:1.针刺对糖尿病心肌病大鼠体重的影响:14周时各组与正常组比有极显著差异(P<0.01),针刺治疗组与模型组、依那普利治疗组比有显著差异(P<0.05)。
2.针刺对糖尿病心肌病大鼠血糖的影响:14周时与正常组比有显著差异(P<0.01),针刺治疗组与模型组相比有极显著差异(P<0.01)。
3.针刺对TNF-α的影响:模型组、针刺治疗组与正常组相比,均有显著性差异(P<0.01),针刺治疗组与模型组相比有显著差异(P<0.01)。
4.针刺对心肌超微结构的改变:与模型组比较,肌丝束排列尚整齐,线粒体纵行排列,明暗带清楚,少部分糖原聚集,肌丝束溶解程度较轻。
结论:1.选用单次腹腔注射STZ55mg/kg制造糖尿病心肌病大鼠模型方法可靠。2.糖尿病心肌病模型大鼠伴随着TNF-α、血糖水平的升高,心肌超微结构的改变。3.针刺治疗糖尿病心肌病,可通过下调TNF-α及血糖含量,改善心肌结构发挥治疗作用,改善机体机能。
Purpose:The treatment by acupuncture in experimental diabetic cardiomyopathy rats is to observe its general condition, blood glucose, TNF-αchanges, myocardial ultrastructure changes ,and explores the role of TNF-αin diabetic cardiomyopathy, and aims to in further analysis of pathogenesis and principles of acupuncture, providing modern scientific basis for acupuncture treatment of diabetic cardiomyopathy .
Methods: 1. To meet the requirements of male Wistar rats were 58, and were randomly divided into normal control group 8 and the remaining 50 single intraperitoneal injection of 55mg/kg streptozotocin diabetic cardiomyopathy rat model of manufacturing, the model was successful 42 and were randomly divided into model group 14, acupuncture group 14, western medicine group 14. 2. Acupuncture group selected Feishu, spleen, Shenshu, internal relations, Sanyinjiao, epigastric next Yu, acupuncture one time every other day, every 15min, continuous acupuncture for 12 weeks;group of western medicine daily morning under the weight of rats fed with enalapril 1.5mg/kg; normal control group, model group group fed with the same volume daily from drinking water. 3. By radioimmunoassay (RIA), determination of TNF-αcontent; using the conventional biochemical indicators of blood glucose; transmission electron microscope to observe myocardial ultrastructure and photographs; determination of rat body weight before and after comparison of 14 weeks.
Results: 1. Effects of acupuncture on body weight of diabetic rats: 14 weeks in each group compared with the control group were significant different(P <0.01), acupuncture group and model group were significantly different (P <0.05).
2. Effects of acupuncture on blood glucose of diabetic rats: 14 weeks, compared with the control group were significantly different (P <0.01), acupuncture group compared with the model group were significantly different (P <0.01).
3. Effects of acupuncture on TNF-αeffect: the model group, acupuncture group compared with the control group, there were significant differences (P <0.01). Acupuncture group compared with the model group were significantly different (P < 0.01).
4.Changes of acupuncture on myocardial ultrastructure: compared with model group, still neatly arranged tow muscle mitochondria vertical rows, with a clear light and shade, a small number of glycogen accumulation, muscle tow dissolved to a lesser extent.
Conclusion:1. Using a single intraperitoneal injection of 55mg/kg streptozotocin rat model of diabetic cardiomyopathy manufacturing method is reliable.
2. Diabetic rat model of myocardial disease is associated with TNF-α, increased blood sugar levels, myocardial ultrastructure.
3. Acupuncture treatment of diabetic cardiomyopathy, can be reduced TNF-αand blood sugar levels, improve the treatment of myocardial structure to play a role in improving body function.
方法:1.将符合要求的雄性Wistar大鼠58只,随机分为正常对照组8只,其余50只单次腹腔注射链脲佐菌素55mg/kg制造糖尿病心肌病大鼠模型,造模成功42只,随机分为模型组14只、针刺治疗组14只、依那普利治疗组14只。2.针刺治疗组选肺俞、脾俞、肾俞、内关、三阴交、胃脘下俞,每日1次针刺,每次15min,平补平泻,连续针刺12周;依那普利治疗组每日上午根据大鼠体重灌服依那普利1.5mg/kg;正常对照组、模型组每日上午灌服等体积饮用水。3.采用放射免疫分析法(RIA),测定TNF-α含量;应用常规生化指标检测血糖;透射电镜下观察心肌超微结构并拍照;测定大鼠14周末体重进行对比。
结果:1.针刺对糖尿病心肌病大鼠体重的影响:14周时各组与正常组比有极显著差异(P<0.01),针刺治疗组与模型组、依那普利治疗组比有显著差异(P<0.05)。
2.针刺对糖尿病心肌病大鼠血糖的影响:14周时与正常组比有显著差异(P<0.01),针刺治疗组与模型组相比有极显著差异(P<0.01)。
3.针刺对TNF-α的影响:模型组、针刺治疗组与正常组相比,均有显著性差异(P<0.01),针刺治疗组与模型组相比有显著差异(P<0.01)。
4.针刺对心肌超微结构的改变:与模型组比较,肌丝束排列尚整齐,线粒体纵行排列,明暗带清楚,少部分糖原聚集,肌丝束溶解程度较轻。
结论:1.选用单次腹腔注射STZ55mg/kg制造糖尿病心肌病大鼠模型方法可靠。2.糖尿病心肌病模型大鼠伴随着TNF-α、血糖水平的升高,心肌超微结构的改变。3.针刺治疗糖尿病心肌病,可通过下调TNF-α及血糖含量,改善心肌结构发挥治疗作用,改善机体机能。
Purpose:The treatment by acupuncture in experimental diabetic cardiomyopathy rats is to observe its general condition, blood glucose, TNF-αchanges, myocardial ultrastructure changes ,and explores the role of TNF-αin diabetic cardiomyopathy, and aims to in further analysis of pathogenesis and principles of acupuncture, providing modern scientific basis for acupuncture treatment of diabetic cardiomyopathy .
Methods: 1. To meet the requirements of male Wistar rats were 58, and were randomly divided into normal control group 8 and the remaining 50 single intraperitoneal injection of 55mg/kg streptozotocin diabetic cardiomyopathy rat model of manufacturing, the model was successful 42 and were randomly divided into model group 14, acupuncture group 14, western medicine group 14. 2. Acupuncture group selected Feishu, spleen, Shenshu, internal relations, Sanyinjiao, epigastric next Yu, acupuncture one time every other day, every 15min, continuous acupuncture for 12 weeks;group of western medicine daily morning under the weight of rats fed with enalapril 1.5mg/kg; normal control group, model group group fed with the same volume daily from drinking water. 3. By radioimmunoassay (RIA), determination of TNF-αcontent; using the conventional biochemical indicators of blood glucose; transmission electron microscope to observe myocardial ultrastructure and photographs; determination of rat body weight before and after comparison of 14 weeks.
Results: 1. Effects of acupuncture on body weight of diabetic rats: 14 weeks in each group compared with the control group were significant different(P <0.01), acupuncture group and model group were significantly different (P <0.05).
2. Effects of acupuncture on blood glucose of diabetic rats: 14 weeks, compared with the control group were significantly different (P <0.01), acupuncture group compared with the model group were significantly different (P <0.01).
3. Effects of acupuncture on TNF-αeffect: the model group, acupuncture group compared with the control group, there were significant differences (P <0.01). Acupuncture group compared with the model group were significantly different (P < 0.01).
4.Changes of acupuncture on myocardial ultrastructure: compared with model group, still neatly arranged tow muscle mitochondria vertical rows, with a clear light and shade, a small number of glycogen accumulation, muscle tow dissolved to a lesser extent.
Conclusion:1. Using a single intraperitoneal injection of 55mg/kg streptozotocin rat model of diabetic cardiomyopathy manufacturing method is reliable.
2. Diabetic rat model of myocardial disease is associated with TNF-α, increased blood sugar levels, myocardial ultrastructure.
3. Acupuncture treatment of diabetic cardiomyopathy, can be reduced TNF-αand blood sugar levels, improve the treatment of myocardial structure to play a role in improving body function.
引文
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[2] Barzilay JI,Abraham L,Heckbert SR,et al.The relation of markers of inflammation to the development of glucose disorders in the el-derly:the cardiovascular health study[J].Diabetes,2001,50(10):2384～2389
[3] Pradhan AD,Manson JE,Rifai N,et al.C reactive protein,interleu-kin 6,and risk of developing type 2 diabetes mellitus[J].JAMA,2001,286(18):2233
[4] Lyon CJ,Law RE,Hsueh WA.Minireview:adiposity,inflammation,and therogenesis.Endocrinology,2003,144(6):2195～2200
[5] Mishima Y,Kuyama A,Tada A,etal.Relationship between serum tumounecrosis factor-alpha and insulin resistance in obesemen with type 2 diabetesmellitus[J].Diabetes Res Clin Pract,2001,52(2):119～123
[6] Kubaszek A,Pihlajamaki J,Komarovski V,etal.Promoter polymorphisms of the TNF-alpha(G-308A) and IL-6(C-174G) genes predict the conversion from impaired glucosed tolerance to type 2 diabetes:the Finnish Diabetes Prevention Study[J].Diabetes,2003,52(7):1872～1876
[7] Qi C Pekala PH.Tumounecrosis factor-alpha-induced insulin res-istance in adipocytes [J]Proc Soc Exp Biol Med,2000,223(2):128~135
[8] Hundal RS,Petersen KF,Mayerson AB,etal.Mechanism by which high-dose aspirin improves glucose metabolism in type 2 diabetes [J].J Clin Invest,2002,109(10):1321~1326
[9]魏群利.针灸治疗非胰岛素依赖性糖尿病临床与实验研究,2001,南京中医药大学博士论文
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