利用杆状病毒表达感染性猪繁殖与呼吸综合征病毒的研究
|
摘要
猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)是猪繁殖与呼吸综合征病原,可引致怀孕母猪繁殖障碍和仔猪呼吸道疾病。该病毒流行广,对养猪业的影响大。目前,我国主要通过疫苗接种预防该病。但该疫苗生产的基质细胞MA104和Marc-145细胞为国外专利垄断。为了免受外国专利限制,开辟了一种新的PRRSV疫苗的呈递途径,本文利用杆状病毒,作了如下研究:
1 PRRSV重组杆状病毒的构建
利用bac-to-bac系统,将PRRSV APRRS全长cDNA重组入杆状病毒中,构建了重组杆状病毒AcAPRRS。AcAPRRS在sf9细胞上连续传5代,通过对嵌合处序列分析,证实AcAPRRS具有较好的稳定性。比较了AcAPRRS与空载体杆状病毒AcLacZ在sf9细胞上的生长曲线,结果显示AcAPRRS具有与AcLacZ相近的生长动力特性。以Northern blot和间接免疫荧光检测了PRRSV基因在sf9细胞中的转录和表达,结果显示,AcAPRRS感染sf9细胞后,有低水平的PRRSV亚基因组转录和N蛋白的表达。上述结果表明,本文获得了稳定的PRRSV重组杆状病毒AcAPRRS。
2重组杆状AcAPRRS产生子代PRRSV的特性研究
以AcAPRRS接种Marc-145细胞,以间接免疫荧光,可检测到PRRSV N蛋白的表达。在Marc-145上清及传代上清中,均可检测到PRRSV序列。分析了子代病毒vASM在Marc-145上一代和二代的5’和3’末端序列,结果显示,vASM具有与分离株相同的基因组末端序列。比较了vASM与亲本病毒APRRS的生长特性,结果显示,vASM与APRRS相同的空斑形态,vASM的生长曲线也与APRRS的相近。上述结果表明,杆状病毒能不影响呈递产生的子代病毒的生物学特性,是理想的外源病毒基因组序列呈递载体。
3重组杆状病毒AcAPRRS转导PRRSV非敏感细胞系的研究
以AcAPRRS接种BHK-21细胞和vero细胞,间接免疫荧光显示,BHK-21细胞和vero细胞中部分存在PRRSV N蛋白的表达。以BHK-21细胞和vero细胞上清接种Marc-145细胞,2d后即出现CPE。以RT-PCR检测了BHK-21细胞和vero细胞上清及传代Marc-145细胞的上清,均存在PRRSV序列。测定了AcAPRRS接种后,BHK-21细胞和vero细胞上产生PRRSV的滴度曲线,BHK-21细胞和vero细胞产生的PRRSV的滴度最高可达104.5 TCID50/ml。上述结果表明,杆状病毒可向细胞呈递其非敏感的病毒基因组序列并产生病毒,为开拓PRRSV疫苗新的呈递途径打下良好基础。
Porcine reproductive and respiratory syndrome virus (PRRSV) is causative agent of porcine reproductive and respiratory syndrome, which is characterized by the abortion and infertility in infected sows, and the increased mortality of piglets from respiratory disease. PRRS has been epidemic in the global swine population, and then resulted in enormously economical cost for the swine industry. Today, PRRS is prevented by innoculated with the vaccine against PRRSV in the mainland China. However, the cells used to product the vaccine were limited by foreign patent. To be free from the patent, a new vaccine against PRRSV should be developed. Here, the baculovirus was used to develop a new vaccine.
1. Construction of the recombinant baculovirus with PRRSV
A recombinant baculovirus, AcAPRRS, was constructed by the genomic cDNA of PRRSV APRRS strain and bac-to-bac system. After 5 times passage of AcAPRRS on sf9 cells, the chimeric sequence in AcAPRRS between baculovirus genome and PRRSV cDNA was amplified, and then analysed. It was confirmed that AcAPRRS was of good stability. The growth curve resulted from AcAPRRS and AcLacZ, the vector baculovirus as controlled virus, on sf9 cells showed that AcAPRRS was of the similar dynamic of growth as AcLacZ. The transcription and expression of PRRSV genome on sf9 cell was detected by Northern blot and indirect immune fluroscence assay. There was low level of transcripted subgenome and N protein in sf9 innoculated with AcAPRRS. These results showed that a stable recombinant baculovirus was obtained.
2. Characterization of PRRSV derived from AcAPRRS
Marc-145 cells were innoculated with AcAPRRS. The PRRSV N protein was expressed in the cells detected by IFA. Three days post innoculation, Marc-145 cells had obviously cytopathic effect. The PRRSV RNA was detected in the supernatant and the second passage supernatant of Marc-145 cells. The end sequences of the offspring virus, vASM , derived from AcAPRRS were analysed by 5’- Race and 3’-Race. vASM was of the same end sequences as the isolates of PRRSV typeⅡ. The plaque morphology and growth dynamic of vASM were almostly indistinguishable from those of APRRS in Marc-145 cells. These results showed that baculovirus could have no effect on the characterization of the offspring virus derived from itself and be ideal vector for delivery of heterologous gene.
3. Study of transduction of AcAPRRS into cells nonsusceptive to PRRSV
BHK-21 cells and vero cells were innoculated with AcAPRRS. The PRRS N protein was expressed in some cells detected by IFA. Marc-145 cells innoculated with the supernatant of BHK-21 or vero could have cytopathic effect post two days. The PRRSV RNA was detected in the supernatant of BHK-21 cell, vero cells and Marc-145 cells. The growth curves of PRRSV producted on BHK-21 cells and vero cells were determinded. The highest titres of PRRSV was up to 104.5 TCID50/ml. These results showed that a virus would be generated in its nonsusceptive cells into which viral genome were deliveried by recombinant baculovirus. Thus, a new pathway was opened up to develop vaccine against PRRSV.
1 PRRSV重组杆状病毒的构建
利用bac-to-bac系统,将PRRSV APRRS全长cDNA重组入杆状病毒中,构建了重组杆状病毒AcAPRRS。AcAPRRS在sf9细胞上连续传5代,通过对嵌合处序列分析,证实AcAPRRS具有较好的稳定性。比较了AcAPRRS与空载体杆状病毒AcLacZ在sf9细胞上的生长曲线,结果显示AcAPRRS具有与AcLacZ相近的生长动力特性。以Northern blot和间接免疫荧光检测了PRRSV基因在sf9细胞中的转录和表达,结果显示,AcAPRRS感染sf9细胞后,有低水平的PRRSV亚基因组转录和N蛋白的表达。上述结果表明,本文获得了稳定的PRRSV重组杆状病毒AcAPRRS。
2重组杆状AcAPRRS产生子代PRRSV的特性研究
以AcAPRRS接种Marc-145细胞,以间接免疫荧光,可检测到PRRSV N蛋白的表达。在Marc-145上清及传代上清中,均可检测到PRRSV序列。分析了子代病毒vASM在Marc-145上一代和二代的5’和3’末端序列,结果显示,vASM具有与分离株相同的基因组末端序列。比较了vASM与亲本病毒APRRS的生长特性,结果显示,vASM与APRRS相同的空斑形态,vASM的生长曲线也与APRRS的相近。上述结果表明,杆状病毒能不影响呈递产生的子代病毒的生物学特性,是理想的外源病毒基因组序列呈递载体。
3重组杆状病毒AcAPRRS转导PRRSV非敏感细胞系的研究
以AcAPRRS接种BHK-21细胞和vero细胞,间接免疫荧光显示,BHK-21细胞和vero细胞中部分存在PRRSV N蛋白的表达。以BHK-21细胞和vero细胞上清接种Marc-145细胞,2d后即出现CPE。以RT-PCR检测了BHK-21细胞和vero细胞上清及传代Marc-145细胞的上清,均存在PRRSV序列。测定了AcAPRRS接种后,BHK-21细胞和vero细胞上产生PRRSV的滴度曲线,BHK-21细胞和vero细胞产生的PRRSV的滴度最高可达104.5 TCID50/ml。上述结果表明,杆状病毒可向细胞呈递其非敏感的病毒基因组序列并产生病毒,为开拓PRRSV疫苗新的呈递途径打下良好基础。
Porcine reproductive and respiratory syndrome virus (PRRSV) is causative agent of porcine reproductive and respiratory syndrome, which is characterized by the abortion and infertility in infected sows, and the increased mortality of piglets from respiratory disease. PRRS has been epidemic in the global swine population, and then resulted in enormously economical cost for the swine industry. Today, PRRS is prevented by innoculated with the vaccine against PRRSV in the mainland China. However, the cells used to product the vaccine were limited by foreign patent. To be free from the patent, a new vaccine against PRRSV should be developed. Here, the baculovirus was used to develop a new vaccine.
1. Construction of the recombinant baculovirus with PRRSV
A recombinant baculovirus, AcAPRRS, was constructed by the genomic cDNA of PRRSV APRRS strain and bac-to-bac system. After 5 times passage of AcAPRRS on sf9 cells, the chimeric sequence in AcAPRRS between baculovirus genome and PRRSV cDNA was amplified, and then analysed. It was confirmed that AcAPRRS was of good stability. The growth curve resulted from AcAPRRS and AcLacZ, the vector baculovirus as controlled virus, on sf9 cells showed that AcAPRRS was of the similar dynamic of growth as AcLacZ. The transcription and expression of PRRSV genome on sf9 cell was detected by Northern blot and indirect immune fluroscence assay. There was low level of transcripted subgenome and N protein in sf9 innoculated with AcAPRRS. These results showed that a stable recombinant baculovirus was obtained.
2. Characterization of PRRSV derived from AcAPRRS
Marc-145 cells were innoculated with AcAPRRS. The PRRSV N protein was expressed in the cells detected by IFA. Three days post innoculation, Marc-145 cells had obviously cytopathic effect. The PRRSV RNA was detected in the supernatant and the second passage supernatant of Marc-145 cells. The end sequences of the offspring virus, vASM , derived from AcAPRRS were analysed by 5’- Race and 3’-Race. vASM was of the same end sequences as the isolates of PRRSV typeⅡ. The plaque morphology and growth dynamic of vASM were almostly indistinguishable from those of APRRS in Marc-145 cells. These results showed that baculovirus could have no effect on the characterization of the offspring virus derived from itself and be ideal vector for delivery of heterologous gene.
3. Study of transduction of AcAPRRS into cells nonsusceptive to PRRSV
BHK-21 cells and vero cells were innoculated with AcAPRRS. The PRRS N protein was expressed in some cells detected by IFA. Marc-145 cells innoculated with the supernatant of BHK-21 or vero could have cytopathic effect post two days. The PRRSV RNA was detected in the supernatant of BHK-21 cell, vero cells and Marc-145 cells. The growth curves of PRRSV producted on BHK-21 cells and vero cells were determinded. The highest titres of PRRSV was up to 104.5 TCID50/ml. These results showed that a virus would be generated in its nonsusceptive cells into which viral genome were deliveried by recombinant baculovirus. Thus, a new pathway was opened up to develop vaccine against PRRSV.
引文
1.王永志,曹三杰,文心田.猪繁殖与呼吸综合征病毒的分子生物学研究进展.动物医学进展, 2005, 26(4): 50-53
2.史喜菊,韩春来,夏春,汪明.猪繁殖与呼吸综合征病毒基因组遗传变异分析.动物医学进展,2003, 24(3): 58—60 [211
3.刘正山,张成,卢锡林。杆状病毒转导不同哺乳动物骨髓来源间充质干细胞。生理学报,2008, 60(3): 431-436
4.孙修炼,胡志红,彭辉银.杆状病毒生态学研究进展.中国病毒学, 2006, 21(2): 200-206.
5.江云波,肖少波,方六荣等.共表达猪繁殖与呼吸综合征病毒修饰型GP5和M蛋白增强DNA疫苗免疫应答的研究.病毒学报,2007, 23(2): 130-136
6.希尼尼根,程安春,汪铭书,等.猪繁殖与呼吸综合征的研究进展.养猪, 2004 , (4) :36238.
7.张建武,庄金山,袁世山.中国部分地区“猪高热综合征”的病原学调查及高致病性猪繁殖与呼吸综合征病毒的分子流行病学研究.中国农业科学, 2008, 41(6):1822-1831
8.李兴玉.猪生殖呼吸综合症病毒分子生物学研究进展四川畜牧兽医2003,30(154):45-47
9.李真光,武华.猪繁殖与呼吸综合征病毒基因组遗传变异研究进展.动物医学进展,2009, 30:101-105
10.李祥健,张建武,吕健,余丹丹,姚火春,袁世山.猪繁殖与呼吸综合征病毒嵌合感染性克隆的构建和应用.生物工程学报, 2008, 24(9):1573-1581
11.杨金雨,仇华吉,童光志.猪繁殖与呼吸综合征疫苗研究进展.动物医学进展. 2005, 26(5): 28-31
12.陈小芳,陈元仲,邹起练等。纤维连接蛋白细胞结合区功能多肽在杆状病毒表达系统中的表达与纯化。生物技术通讯,2008,19(4): 515-517
13.郑其升,李鹏,毕志香等. PRRSV NJ-a株ORF5基因A表位的修饰与糖基化位点的突变对其DNA疫苗免疫效力的影响.生物工程学报, 2007, 23(1):33-39
14.信爱国,李华春,廖德芳等。亚洲1型口蹄疫病毒衣壳蛋白前体P1—2A基因和蛋白酶3C基因在昆虫细胞中的共表达。中国人兽共患病学报,2008,24(6):500-504
15.赵昆,刘思国,王春来等。牛抗菌肽Bac7-Bac5-βdefense串联基因在昆虫杆状病毒系统中的表达及其产物的活性分析。农业生物技术学报,2008,16(3):380-384
16.殷震,刘景华.动物病毒学[M].北京,科学出版社,1997,613-627
17.郭保清,陈章水,刘文兴等.从疑似PRRS流产胎儿中分离PRRSV的研究[J].中国畜禽传染病, 1996, 2:1-5.
18.崔保安等.猪繁殖与呼吸综合症病毒分子生物学研究进展[J].动物医学进展,2004,25(1):22-24
19. Ahrens CH, Carlson C, Rohrmann GF. Identification, sequence, and transcriptional analysis of lef-3, a gene essential for Orgyia pseudotsugata baculovirus DNA replication. ogy. 1995, 210(2):372-82.
20. Albina E, Madec F, Cariolet R, Torrison JL. Immune response and persistence of the porcinereproductive and respiratory syndrome virus in infected pigs and farm units. Vet Rec, 1994, 134:567–73.
21. Albina E. Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview. Vet Microbiol. 1997, 55:309-316.
22. Allende R, Laegreid W, Kutish G, Galeota J, Wills RW, Osorio FA. Porcine reproductive and respiratory syndrome virus: description of persistence in individual pigs upon experimental infection. J Virol, 2003, 72:10834–7.
23. Arends HM, Jehle JA. Homologous recombination between the inverted terminal repeats of defective transposon TCp3.2 causes an inversion in the genome of Cydia pomonella granulovirus. J Gen Virol. 2002, 83(Pt 7):1573-8
24. Batista L, Dee SA, Rossow KD, Deen J, Pijoan C. Assessing the duration of persistence and shedding of porcine reproductive and respiratory disease syndrome virus in a large population of breeding-age gilts. Can J Vet Res, 2002, 66:196–200.
25. Benfield DA, Christopher-Hennings J, Nelson EA, Rowland RRR, Nelson JK. Persistent fetal infection of porcine repro-ductive and respiratory syndrome virus infection. In: Proceed-ings of the 28th Ann Meet Am Assoc Swine Prac, 1997. 455–8.
26. Benfield DA, Nelson E, Collins JE, et al. Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J. Vet. Diagn. Invest. 1992, 4: 127-133.
27. Bierk MD, Dee SA, Rossow KD, Otake S, Collins JE, Molitor TW. Transmission of PRRS virus frompersistently infected sows to contact controls. Can J Vet Res 2001, 65: 261–6.
28. Bilello, J.P. et al. Transient disruption of intracellular junctions enables baculovirus entry into nondividing hepatocytes. J. Virol. 2001, 75: 9857–9871
29. Blissard GW, Rohrmann GF. Baculovirus diversity and molecular biology. Annu Rev Entomol. 1990, 35: 127-55.
30. Boublik Y., Di Bonito, P. and Jones, I. M.‘Eukaryotic virus display: Engineering the major surface glycoprotein of the Autographa californica nuclear polyhedrosis virus (AcNPV) for the presentation of foreign proteins on the virus surface’, Biotechnology, 1995, 13: 1079–1084.
31. Boyce FM, Bucher NLR. Baculovirus-mediated gene transfer into mammalian cells. Proc Natl Acad Sci USA 1996, 93: 2348–2352.
32. Broer R, Heldens JG, van Strien EA. Specificity of multiple homologous genomic regions in Spodoptera exigua nucleopolyhedrovirus DNA replication. J Gen Virol. 1998, 79 ( Pt 6): 1563-72
33. Carbonell LF, Klowden MJ, Miller LK. Baculovirus-mediated expression of bacterial genes in dipteran and mammalain cells. J Virol 1985, 56: 153–60.
34. Cary LC, Goebel M, Corsaro BG, Wang HG, Rosen E, Fraser MJ. Transposon mutagenesis of baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses. Virology. 1989, 172(1): 156-69.
35. Cavanagh D. Nidovirales: a new order comprising Coronaviridae and Arteriviridae . Arch Virol, 1997, 142(2): 629–33.
36. Cha HJ, Pham MQ, Rao G, Bentley WE. Expression of green fluorescent protein in insect larvae and its application for heterologous protein production. Biotechnol Bioeng 1997; 56: 239–247.
37. Chapple, S. D. and Jones, I. M.‘Non-polar distribution of green ?uorescent protein on the surface of Autographa californica nucleopolyhedrovirus using a heterologous membrane anchor’, J. Biotechnol., 2002, 95: 269–275.
38. Cheng T, Xu CY, Wang YB, Chen M, Wu T, Zhang J, et al. A rapid and efficient method to express target genes in mammalian cells by baculovirus. World J Gastroenterol 2004, 10: 1612–18.
39. Chris J N, Michael P M, Dkay S F. Porcine reproductive and respiratory syndrome virus Comparison: Divergent evolution on two continents [J]. J Virol, 1999,73(1): 270–280.
40. Christian Oker-Blom, Kari J. Airenne and Reingard Grabherr. Baculovirus display strategies: Emerging tools for eukaryotic libraries and gene delivery. BRIEFINGS IN FUNCTIONAL GENOMICS AND PROTEOMICS. 2003, 2: 244–253.
41. Clay WC, Condreay JP, Moore LB, Weaver SL, Watson MA, Kost TA, et al. Recombinant baculoviruses used to study estrogen receptor function in human osteosarcoma cells. Assay Drug Dev Technol 2003, 1: 801–10.
42. Collins, J. E., Benfield, D. A., Christianson, W. T., Harris, L., Hennings, J. C., Shaw, D. P., Goyal, S. M., McCullough, S., Morrison, R. B., Joo, H. S., Gorcyca, D. E., and Chladek, D. W. Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J. Vet. Diagn. Invest. 1992, 4: 117-126.
43. Condreay JP, Witherspoon SM, Clay WC, Kost TA. Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc Natl Acad Sci USA 1999, 96: 127–32.
44. Conner M, Zarley C, Hu B, Parsons S, Drabinski D, Greiner S, et al. Virus-like particles as a rotavirus subunit vaccine. J Infect Dis, 1996, 174: 88–92.
45. Conzelmann K K, Visser N, van Woesel P, et al. Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group [J]. Virology, 1993, 193(1): 329–339.
46. Cristina Fipaldini, Barbara Bellei, and Nicola La Monica. Expression of Hepatitis C Virus cDNA in Human Hepatoma Cell Line Mediated by a Hybrid Baculovirus±HCV Vector. Virology, 1999, 255: 302-311.
47. Crouch EA, Passarelli AL. Genetic requirements for homologous recombination in Autographa californica nucleopolyhedrovirus. J Virol. 2002, 76(18): 9323-34
48. Dea S, Gagnon C A, Mardassi H et al. Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus: comparison of the North American and European isolates[J]. Arch. Virol, 2000, 145: 659–688.
49. Delaney, W. E., & Isom, H. C. Hepatitis B virus replication in human HepG2 cells mediated byhepatitis B virus recombinant baculovirus. Hepatology , 1998, 28: 1134–1146
50. Delputte, P. L., Nauwynck, H. J. Porcine Arterivirus Infection of Alveolar Macrophages Is Mediated by Sialic Acid on the Virus. J. Virol. 2004, 78: 8094-8101
51. Ding-Gang Yang, Yao-Chi Chung, Yiu-Kay Lai, et al. Avian Infuenza Virus Hemagglutinin Display on Baculovirus Envelope: Cytoplasmic Domain Affects Virus Properties and Vaccine Potential. Molecular Therapy, 2007, 15: 5989–5996
52. Duffy SP, Young AM, Morin B, et al. Sequence analysis and organization of the Neodiprion abietis nucleopolyhedrovirus genome. J Virol. 2006, 80(14): 6952-63
53. Duisit G, Saleun S, Douthe S, Barsoum J, Chadeuf G, Moullier P. Baculovirus vector requires electrostatic interactions includingheparan sulfate for efficient gene transfer in mammalian cells. J Gene Med 1999, 1: 93–102.
54. Elmar A Joura, Sepp Leodolter, Mauricio Hernandez-Avila, et al. E?cacy of a quadrivalent prophylactic human papillomavirus (types 6, 11, 16, and 18) L1 virus-like-particle vaccine against high-grade vulval and vaginal lesions: a combined analysis of three randomised clinical trials. Lancet, 2007; 369: 1693–702
55. Eric JS, Janneke JMM. The molecular biology of arteriviruses [J]. J Gen Virol, 1998, 79: 961-9791.
56. Facciabene A., Aurisicchio L. and La Monica N. Baculovirus vectors elicit antigen-specific immune responses in mice. J. Virol., 2004, 78: 8663-8672
57. Fauguet CM., Mayo MA., Maniloff J., et al. Virus Taxonomy: the Eighth Report of the International Committee on Taxonomy of Viruses. 2005, New Youk
58. Fipaldini C, Bellei B, La Monica N. Expression of hepatitis C virus cDNA in human hepatoma cell line mediated by a hybrid baculovirus-HCV vector. Virology, 1999; 255: 302–11.
59. Fraser MJ, Ciszczon T, Elick T, et al. Precise excision of TTAA-specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera. Insect Mol Biol. 1996, 5(2): 141-51.
60. Free In PMC, Virological and immunological responses to Porcine reproductive and respiratory syndrome virus In a large population of gilts. Can J Vet Res.,2004, 68(4): 267-73.
61. Friesen PD, Nissen MS. Gene organization and transcription of TED, a lepidopteran retrotransposon integrated within the baculovirus genome. Mol Cell Biol. 1990, 10(6): 3067-77
62. Friesen PD, Rice WC, Miller DW, Miller LK. Bidirectional transcription from a solo long terminal repeat of the retrotransposon TED: symmetrical RNA start sites. Mol Cell Biol. 1986, 6(5): 1599-607
63. Gerhardt E, Bruss V. Phenotypic mixing of rodent but not avian hepadnavirus surface proteins into human hepatitis B virus particles. J Virol 1995; 69: 1201–8.
64. Gronowski, A.M. et al. Baculovirus stimulates antiviral effects in mammalian cells. J. Virol. 1999, 73: 9944–9951
65. Guarino LA, Gonzalez MA, Summers MD. Complete Sequence and Enhancer Function of the Homologous DNA Regions of Autographa californica Nuclear Polyhedrosis Virus. J Virol. 1986,60(1): 224-229.
66. Guarino LA, Jin. J and Dong W. Guanylyltransferase activity of the LEF-4 subunit of baculovirus RNA polymerase. J Virol. 1998, 72(12): 10003-10.
67. HAIYING YU, OLAF ISKEN, CLAUS W. GRASSMANN, et al. A Stem-Loop Motif Formed by the Immediate 59 Terminus of the Bovine Viral Diarrhea Virus Genome Modulates Translation as well as Replication of the Viral RNA. J. VIROL., 2000, 74(13): 5825–5835
68. Hajek KL, Friesen PD. Proteolytic processing and assembly of gag and gag-pol proteins of TED, a baculovirus-associated retrotransposon of the gypsy family. J Virol. 1998 ,72(11): 8718-24
69. Hajós JP, Pijnenburg J, Usmany M, et al. High frequency recombination between homologous baculoviruses in cell culture. Arch Virol. 2000, 145(1): 159-64.
70. Harrison RL, Bonning BC. Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses. J Gen Virol. 2003, 84(Pt 7): 1827-42
71. Hefferon KL, Oomens AG, Monsma SA, Finnerty CM, Blissard GW. Host cell receptor binding by baculovirus GP64 and kinetics of virion entry. Virology. 1999, 258(2): 455-68
72. Herniou EA, Luque T, Chen X, Vlak JM, et al. Use of whole genome sequence data to infer baculovirus phylogeny. J Virol., 2001, 75(17):8117-26
73. Herniou EA, Olszewski JA, O'Reilly DR, Cory JS. Ancient coevolution of baculoviruses and their insect hosts. J virol., 2004, 78: 3244-3251.
74. Hideki Tani, Chang Kwang Limn,Chan Choo Yap, et al. In Vitro and In Vivo Gene Delivery by Recombinant Baculoviruses. J Virol. 2003, 77(18): 9799–9808
75. Ho Y, Lin PH, Liu CYY, Lee SP, Chao YC. Assembly of human severe acute respiratory syndrome coronavirus-like particles. Biochem Biophys Res Commun, 2004, 318: 833–8.
76. Ho Y, Lo HR, Lee TC, Wu CP, Chao YC. Enhancement of correct protein folding in vivo by a non-lytic baculovirus. Biochem J. 2004, 382: 695–702.
77. Ho YC, Chen HC, Wang KC, Hu YC. Highly efficient baculovirus-mediated gene transfer into rat chondrocytes. Biotechnol Bioeng, 2004, 88: 643–51.
78. Hofmann C, Sandig V, Jennings G, Rudolph M, Schlag P, Strauss M. Efficient gene-transfer into human hepatocytes by baculovirus vectors. Proc Natl Acad Sci USA, 1995, 92: 10099–103.
79. Hollister JR, Shaper JH, Jarvis DL. Stable expression of mammalian beta 1,4-galactosyltransferase extends the N-glycosylation pathway in insect cells. Glycobiology 1998, 8: 473–80.
80. Horter DC, Pogranichiny RM, Chang CC, Evans RB, Yoon KJ, Zimmerman JJ. Characterization of the carrier state in porcine reproductive and respiratory syndrome virus infection. Vet Microbiol 2002, 86: 213–28.
81. HU Yu-chen. Baculovirus as a highly efficient expression vector in insect and mammalian cells. Acta Pharmacologica Sinica, 2005, 26 (4): 405–416
82. Hua-Ji Qiu, Zhi-Jun Tian, Guang-Zhi Tong, et al. Protective immunity induced by a recombinant pseudorabies virus expressing the GP5 of porcine reproductive and respiratory syndrome virus in piglets. Veterinary Immunology and Immunopathology, 2005, 106: 309–319
83. Jarvis DL, Aumiller JJ, Hollister JR, Kawar ZS, Pilon J, Vadaie N. Analysis and manipulation of the protein N-glycosylation pathway in the baculovirus-insect cell system. Glycobiology, 2004, 14: 1059–60.
84. Jarvis DL, Fleming J, Kovacs GR, Summers MD, Guarino LA. Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells. Biotechnology 1990, 8: 950–55.
85. Jarvis DL. Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production. Virology, 2003, 310: 1–7.
86. Jehle JA, Blissard GW, Bonning BC, et al. On the classification and nomenclature of baculoviruses: a proposal for revision. Arch Virol. 2006, 151(7): 1257-66.
87. Jehle JA, Fritsch E, Huber J. Intra-specific and inter-specific recombination of tortricid-specific granuloviruses during co-infection in insect larvae. Arch Virol. 2003, 148(7): 1317-33.
88. Jehle JA. The expansion of a hypervariable, non-hr ori-like region in the genome of Cryptophlebia leucotreta granulovirus provides in vivo evidence for the utilization of baculovirus non-hr oris during replication. J Gen Virol. 2002, 83(Pt 8): 2025-34.
89. Jehle JA. The mosaic structure of the polyhedrin gene of the Autographa californica nucleopolyhedrovirus (AcMNPV). Virus Genes, 2004, 29(1): 5-8
90. Jenkinson S, McCoy DC, Kerner SA, Ferris RG, Lawrence WK, Clay WC, et al. Development of a novel high-throughput surrogate assay to measure HIV envelope/CCR5/CD4-mediated viral/cell fusion using BacMam baculovirus technology. J Biomol Screen, 2003, 8: 463–70.
91. Jenny G. Cho, Scott A. Dee. Porcine reproductive and respiratory syndrome virus. Theriogenology, 2006, 66: 655–662
92. John J. Treanor; Gilbert M. Schiff; Frederick G. Hayden; et al. Safety and Immunogenicity of a Baculovirus-Expressed Hemagglutinin Influenza Vaccine: A Randomized Controlled Trial. JAMA, 2007, 297(14): 1577-1582
93. Joo H S ,Park B K,Dee S A ,et al . Indirect fluorescent IgM antibody response ofbigs infected with porcine reproductive and respiratory syndrome virus . Vet Microbiol, 1997, 55: 303-307.
94. Kamita SG, Maeda S, Hammock BD. High-frequency homologous recombination between baculoviruses involves DNA replication. J Virol. 2003, 77(24): 13053-61
95. Kazuhiro Okano, Adam L. Vanarsdall, Victor S. Mikhailov, et al. Conserved molecular systems of the Baculoviridae. Virology, 2006, 344: 77– 87
96. Keffaber KK. Reproductive failure of unknown etiology. Am. Assoc. Swine Prac. Newsl, 1989,1: 1-9.
97. Kitts PA. and Possee RD. A method for producing recombinant baculovirus expression vectors at high frequency. Biotechniques, 1993, 14: 810-817.
98. Kitts PA, Ayres MD and Possee RD. Linearization of baculovirus DNA enhangces the recovery of recombinant virus expression vectors. Nucleic Acids Res., 1990, 18: 5667-5672.
99. Kost TA, Condreay JP. Recombinant baculoviruses as mammalian cell gene delivery vectors.Trends Biotechnol, 2002, 20: 173–80.
100. Kristensen CS, Botner A eatl. Experimental airborne transmission of PRRS virus[J]. Vet Microbiol. 2004, 99(3-4): 197-202
101. Lange M, Wang H, Zhihong H, et al. Toward a molecular indentification and classification system of lepidopteran-specific baculovirus. Virology, 2004, 325: 36-47.
102. Laprise M-H, Grondin F, Dubois CM. Enhanced TGFβ1 maturation in high five cells coinfected with recombinant baculovirus encoding the convertase furin/pace: improved technology for the production of recombinant proproteins in insect cells. Biotechnol Bioeng, 1998, 58: 85–91.
103. Lee C, Yoo D. The small envelope protein of porcine reproductive and respiratory syndrome virus possesses ion channel protein-like properties [J]. Virology, 2006, 355(1): 30–43.
104. Lee C,Rogan D,Erickson L,Zhang J ,Yoo D. Chatacterization of the porcine reproductive and respiratory syndrome virus glycoprotein5(GP5) in stably expressing cells. [J] Virus Res, 2004, 104(1): 33-8
105. Lee H, Krell PJ. Reiterated DNA fragments in defective genomes of Autographa californica nuclear polyhedrosis virus are competent for AcMNPV-dependent DNA replication. Virology, 1994, 202(1): 418-29
106. Lee HY, Krell PJ. Generation and analysis of defective genomes of Autographa californica nuclear polyhedrosis virus. J Virol. 1992, 66(7): 4339-47
107. Lemke C D ,Haynes J S ,Spaete R ,et al. Lymphoid hyperplasia resulting in immune dysregulation is caused by Porcine reproductive and respiratory syndrome virus infection in neonatal pigs . J Immunol, 2004, 172: 1916-1925.
108. Lihoradova OA, Bachurina EY, Azimova SS. Biosynthesis of the recombinant middle surface antigen of the human hepatitis B virus in silkworm larvae. Mol Biol, 2004, 38: 603–7.
109. Lo HR, Chou CC, Wu TY, Yuen JP, Chao YC. Novel baculovirus DNA elements strongly stimulate activities of exogenous and endogenous promoters. J Biol Chem 2002, 277: 5256–64.
110. Lopez OJ., Osorio FA. Role of neutralizing antibodies in PRRSV protective immunity. Veterinary Immunology and Immunopathology, 2004, 102: 155–163
111. Lu A, Miller LK. The roles of eighteen baculovirus late expression factor genes in transcription and DNA replication. J Virol. 1995, 69(2): 975-82.
112. Lucifora J, Durantel D, Belloni L, et al. Initiation of hepatitis B virus genome replication and production of infectious virus following delivery in HepG2 cells by novel recombinant baculovirus vector. J. Gen. Virol. 2008, 89: 1819–1828
113. Luckow VA, Lee SC, Barry GF, et al. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in E. coli. J. Virol., 1993, 67: 4566-4579.
114. Maeda S, Kamita SG, Kondo A. Host range expansion of Autographa californica nuclear polyhedrosis virus (NPV) following recombination of a 0.6-kilobase-pair DNA fragment originating from Bombyx mori NPV. J Virol. 1993, 67(10): 6234-8
115. Marcelo de Lima, Asit K. Pattnaik, Eduardo F. Flores, et al. Serologic marker candidates identified among B-cell linear epitopes of Nsp2 and structural proteins of a North American strain of porcine reproductive and respiratory syndrome virus. Virology, 2006, 353: 410–421
116. Marcelo de Lima, Byungjoon Kwon, Israrul H. Ansari, et al. Development of a porcine reproductive and respiratory syndrome virus differentiable (DIVA) strain through deletion of specific immunodominant epitopes. Vaccine, 2008, 26: 3594–3600
117. McDougal VV, Guarino LA. DNA and ATP binding activities of the baculovirus DNA helicase P143. J Virol. 2001, 75(15): 7206-9.
118. Mengeling, WL., Lager, KM., Vorwald, AC. Clinical consequences of exposing pregnant gilts to strains of porcine reproductive and respiratory syndrome (PRRS) virus isolated from field cases of“atypical”PRRS. Am. J. Vet. Res. 1998, 59: 1540-1544.
119. Merrihew RV, Clay WC, Condreay JP, Witherspoon SM, Dallas WS, Kost TA. Chromosomal integration of transduced recombinant baculovirus DNA in mammalian cells. J Virol, 2001, 75: 903–9.
120. Meulenberg JJ, Hulst MM, de Meijer EJ, et al. Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS) is related to LDV and EAV [J]. Virology, 1993, 192(1): 62–72.
121. MEULENBERG JJM., JNA BOS-DE RUIJTER, R. VAN DE GRAAF, et al. Infectious Transcripts from Cloned Genome-Length cDNA of Porcine Reproductive and Respiratory Syndrome Virus. J. VIROL., 1998, 72(1): 380–387
122. Mikhailov VS. Helix-destabilizing properties of the baculovirus single-stranded DNA-binding protein (LEF-3). Virology. 2000, 270(1): 180-9
123. Monique M. van Oers and Just M. Vlak. Baculovirus Genomics. Current Drug Targets, 2007, 8: 1051-1068
124. Mortola E, Roy P. Efficient assembly and release of SARS coronavirus-like particles by a heterologous expression system. FEBS Lett, 2004, 576: 174–8.
125. Mottershead DG., Alfthan K, Ojala K. et al.‘Baculoviral display of functional scFv and synthetic IgG-binding domains’, Biochem. Biophys. Res. Commun., 2000, 275: 84–90.
126. Murtaugh MP, Elam MR, Kakach LT. Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus [J]. Arch Biol, 1995, 140: 1451–60.
127. Narinder Pal, Sandhya Boyapalle, Randy Beckett, et al. A Baculovirus-Expressed Dicistrovirus That Is Infectious to Aphids. J VIROL, 2007, 81(17): 9339–9345
128. Narinder Pal, Sandhya Boyapalle, Randy Beckett, et al. A Baculovirus-Expressed Dicistrovirus That Is Infectious to Aphids. J. VIROL. 2007, 81(17): 9339–9345
129. Nielsen J, Botner A, Bille Hansen V, et al. Experimental inoculation of late term pregnant sows with a field isolate of porcine reproductive and respiratory syndrome vaccine-derived virus . Vet Microbiol, 2002, 84: 1213.
130. Ohkawa T, Rowe AR and Volkman LE.‘Identification of six Autographa californica multicapsidnucleopolyhedrovirus early genes that mediate nuclear localization of G-actin’, J. Virol., 2002, 76: 2281–12289.
131. Ojala K, Mottershead DG., Suokko A. and Oker-Blom C.‘Specific binding of baculoviruses displaying gp64 fusion proteins to mammalian cells’. Biochem. Biophys. Res. Commun., 2001, 284: 777–784.
132. Okano K, Vanarsdall AL, Mikhailov VS, et al. Conserved molecular systems of the Baculoviridae[J]. Virology, 2006, 344: 77-87.
133. Olson VA, Wetter JA, Friesen PD. The highly conserved basic domain I of baculovirus IE1 is required for hr enhancer DNA binding and hr-dependent transactivation. J Virol. 2003, 77(10): 5668-77.
134. Olszewski J, Miller LK. A role for baculovirus GP41 in budded virus production. Virology. 1997, 233(2): 292-301
135. Oomens AG, Monsma SA, Blissard GW. The baculovirus GP64 envelope fusion protein: synthesis, oligomerization, and processing. Virology. 1995, 209(2): 592-603.
136. Oomens AG. and Blissard GW.‘Requirement for gp64 to drive efficient budding of Autographa californica multicapsid nucleopolyhedrovirus’, Virology, 1999, 254: 297–314.
137. Oppenheimer DI, Volkman LE. Evidence for rolling circle replication of Autographa californica M nucleopolyhedrovirus genomic DNA. Arch Virol. 1997, 142(10): 2107-13.
138. Osorio FA, Zuckermann F, Wills R, Meier W, Christian S, Galeota J, Doster A. PRRSV: comparison of commercial vaccines in their ability to induce protection against current PRRSV strains of high virulence. Allen D. Leman Swine Conference. 1998, 25: 176-182
139. Otake S, Dee SA, Rossow KD, Deen J, Joo HS, Molitor TW, et al. Transmission of porcine reproductive and respiratory syndrome virus by fomites (boots and coveralls). Swine Health Prod ,2002, 10: 59–65.
140. Palhan VB, Sumathy S, Gopinathan KP. Baculovirus mediated high-level expression of luciferase in silkworm cells and larvae. Biotechniques, 1995, 19: 97–104.
141.Patterson RM, Selkirk JK, Merrick BA. Baculovirus and insect cell gene expression-review of baculovirus biotechnology. Environ Health Perspect, 1995, 103: 756–9.
142. Pearson MN, Rohrmann GF. Lymantria dispar nuclear polyhedrosis virus homologous regions: characterization of their ability to function as replication origins. J Virol. 1995, 69(1): 213-21
143. Pearson MN, Russell RL, Rohrmann GF, Beaudreau GS. p39, a major baculovirus structural protein: immunocytochemical characterization and genetic location. Virology. 1988, 167(2): 407-13
144. Pijlman GP, Dortmans JC, Vermeesch AM, et al. Pivotal role of the non-hr origin of DNA replication in the genesis of defective interfering baculoviruses. J Virol. 2002, 76(11): 5605-11.
145. Pijlman GP, van den Born E, Martens DE. Autographa californica baculoviruses with large genomic deletions are rapidly generated in infected insect cells. Virology. 2001, 283(1): 132-8.
146. Pijlman GP, van Schijndel JE, and Vlak JM. Spontaneous excision of BAC vector sequences from bacmid-derived baculovirus expressio vectors upon passage in insect cells. J. Gen. Virol., 2003, 84:2669-2678.
147. Pirzadeh B, Dea S .Immune response in pigs vaccinated with plasmid DNA encoding ORF5 of porcine reproductive and respiratory syndrome virus. Journal of General Virology, 79: 989-999
148. Plagemann PG, Rowland RR. Polyclonal hypergammaglobulinemia and formation of hydrophobic immune complexes in porcine reproductive and respiratory syndrome virus-infected and uninfected pigs [J] Viral Immunol. 2005, 18(1): 138-47
149. Plana Duran ,Collins J E ,Mengling WL ,et al . Efficacy of an inactivated vaccine for Prevention of reproductive failure induced by porcine reproductive and respiratory syndrome virus .Vet Microbiol , 1997 , 55: 361-370.
150. Planaduran J,Climent I,sarraseca J, et al. Baculovrius expression of proteins of porcine reproductive and respiratory syndrome virus st rain Olot/ 91 Involvement of ORF3 and ORF5 proteins in protection . Virus Genes ,1997 ,14: 19229.
151. Raju TS, Lerner L, O’Connor JV. Glycopinion: biological significance and methods for the analysis of complex carbohydrates of recombinant glycoproteins. Biotechnol Appl Biochem., 1996, 24: 191–4
152. Robert Strauss, Andreas Huser, Shaoheng Ni, et al. Baculovirus-based Vaccination Vectors Allow for Efficient Induction of Immune Responses Against Plasmodium falciparum Circumsporozoite Protein. Molecular Therapy, 2007, 15: 193–202
153. Rohrmann GF. Polyhedrin structure. J Gen Virol. 1986, 67 ( Pt 8): 1499-513.
154. Russell RL, Pearson MN and Rohrmann GF.‘Immunoelectron microscopic examination of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus-infected Lymantria dispar cells: Time course and localization of major polyhedron- associated proteins’, J. Gen. Virol., 1991, 72: 275–283.
155. Sarkis C, Serguera C, Petres S, Buchet D, Ridet JL, Edelman L, et al. Efficient transduction of neural cells in vitro and in vivo by a baculovirus-derived vector. Proc Natl Acad Sci USA, 2000, 97: 14638–43.
156. Shoji I, Aizaki H, Tani H, Ishii K, Chiba T, Saito I, et al. Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors. J Gen Virol, 1997, 78: 2657–64.
157. Siao-Kun Wan Welch, Rika Jolie, Douglas S. Pearce, et al. Construction and evaluation of genetically engineered replication-defective porcine reproductive and respiratory syndrome virus vaccine candidates. Veterinary Immunology and Immunopathology, 2004, 102: 277–290
158. Smith G. P. Filamentous fusion phage: novel expression vector that display cloned antigens on the virion surface[J]. Science, 1985, 228: 1315-1317.
159. Smith GE., Summers MD. and Fraser MJ. Pruduction of human beta interferon in insect cells infected with a baculovirus expression vector. Mol. Cell. Biol., 1983, 3: 2156-2165
160. Snijder E J and Meulenberg J J M. The molecular biology of arteriviruses[J]. J Gen Virol, 1998, 79: 961-979.
161. Spenger, A., Grabherr, R., Tollner, L. et al.‘Altering the surface properties of baculovirusAutographa californica NPV by insertional mutagenesis of the envelope protein gp64’, Eur. J. Biochem. 2002, 269: 4458–4467.
162. Steinhaus EA. Duration of infectivity of the virus of silkworm jaundice. Science, 1954, 120: 186-187.
163. Tami C, Farber M, Palma EL. and Taboga O.‘Presentation of antigenic sites from foot-and-mouth disease virus on the surface of baculovirus and in the membrane of infected cells’, Arch. Virol., 2000, 145: 1815–1828.
164. Tani, H. et al. Characterization of cell surface determinants important for baculovirus infection. Virology, 2001, 279: 343–353
165. Thiem SM, Miller LK. Identification, sequence, and transcriptional mapping of the major capsid protein gene of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol. 1989 , 63(5): 2008-18.
166. Thomas A Kost, J Patrick Condreay and Donald L Jarvis. Baculovirus an versatile vectors for protein expression in insect and mammalianm cells. Nature Biotechnology, 2005, 23(5): 567-575
167. Thomas A. Kost and J. Patrick Condreay. Baculovirus Expression Vectors for Insect and Mammalian Cells. Current Drug Targets, 2007, 8: 1126-1131
168. Thomas A. Kost and J. Patrick Condreay. Recombinant baculoviruses as mammalian cell gene-delivery vectors. TRENDS in Biotechnology, 2002, 20 (4): 173-180
169. Tian K, Yu X, Zhao T, et al. Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark [J]. PLoS ONE, 2007,2(6): 526.
170. Tjia, ST., G. M. zu Altenschildesche, and W. Doer?er. Autographa californica nuclear polyhedrosis virus (AcNPV) DNA does not persist in mass cultures of mammalian cells. Virology, 1983, 125: 107–117.
171. Todd JW, Passarelli AL, Lu A, Miller LK. Factors regulating baculovirus late and very late gene expression in transient-expression assays. J Virol. 1996, 70(4): 2307-17
172. Urabe M., Ding C. and Kotin RM. Insect cells as a factory to produce adeno-associated virus type 2 vectors , 2002, Hum. Gene Ther., 13(16): 1935-1943.
173. van Loo, N.D. et al. Baculovirus infection of non-dividing mammalian cells: mechanisms of entry and nuclear transport of capsids. J. Virol. 2001, 75: 961–970
174. van Oers MM, Vlak JM. Baculovirus genomics. Curr Drug Targets. 2007, 8(10): 1051-68.
175. Vanarsdall AL, Okano K, Rohrmann GF. Characterization of the replication of a baculovirus mutant lacking the DNA polymerase gene. Virology, 2005, 331(1): 175-80
176. Venkaiah B, Viswanathan P, Habib S, Hasnain SE. An additional copy of the homologous region (hr1) sequence in the Autographa californica multinucleocapsid polyhedrosis virus genome promotes hyperexpression of foreign genes. Biochemistry, 2004, 43: 8143–51.
177. Viswanathan P, Venkaiah B, Kumar MS, et al. The homologous region sequence (hr1) of Autographa californica multinucleocapsid polyhedrosis virus can enhance transcription from non-baculoviral promoters in mammalian cells. J Biol Chem, 2003, 278: 52 564–71.
178. Vlak J M., Schoutten A., Usmany M., et al. Expression of cauliflower mosaic virus geneⅠusing a baculovirus based upon the p10 gene and a novel selection method. Virology, 1990, 179: 312-320.
179. Volkman LE, Goldsmith PA. In vitro study of Autographa californica nuclear polyhedrosis virus interaction with nontarget vertebrate host cells. Appl Environ Microbiol, 1983, 45: 1085–1093.
180. Wagle M, Jesuthasan S. Baculovirus-mediated gene expression in zebrafish. Marine Biotechnol 2003, 5: 58–63.
181. Wang KC, Wu JC, Chung YC, et al. Baculovirus as a highly efficient gene delivery vector for the expression of hepatitis delta virus antigens in mammalian cells. Biotechnol Bioeng, 2005, 89: 464-73.
182. Wang Y, Liang Y, Han J, et al. Attenuation of porcine reproductive and respiratory syndrome virus strain MN184 using chimeric construction with vaccine sequence. Virology, 2008, 371(2): 418-29.
183. Wensvoort G, Terpstra C, Pol JM, et al. Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Veterinary Quarterly, 1991, 13: 121-130.
184. Wills RW, Zimmerman JJ, Yoon KJ, et al. Porcine reproductive and respiratory syndrome virus: a persistent infection. Vet Microbiol, 1997, 55: 231–40.
185. Wissink EHJ, MV Kroese, HAR. van Wijk, et al. Envelope Protein Requirements for the Assembly of Infectious Virions of Porcine Reproductive and Respiratory Syndrome Virus. J. VIROL., 2005, 79(19): 12495–12506
186. Wootton S, Yoo D, Rogan D. Full-length sequence of a Canadian porcine reproductive and respiratory syndrome virus (PRRSV) isolate. Arch Virol, 2000, 145(11): 2297–2323.
187. Xiangjie Yao, Qingxia Han , Jianhua Song , et al. Baculovirus Mediated Production of Infectious Hepatitis C Virus in Human Hepatoma Cells Stably Expressing T7 RNA Polymerase. Mol Biotechnol, 2008, 40: 186–194
188. Yu-chen HU Baculovirus as a highly efficient expression vector in insect and mammalian cells. Acta Pharmacologica Sinica, 2005, 26 (4): 405–416
189. Ziebuhr J, Snijder E J and Gorbalenya A E. Virus-encoded proteinases and proteolytic processing in the Nidovirales[J]. J Gen Virol, 2000, 81: 853-879
2.史喜菊,韩春来,夏春,汪明.猪繁殖与呼吸综合征病毒基因组遗传变异分析.动物医学进展,2003, 24(3): 58—60 [211
3.刘正山,张成,卢锡林。杆状病毒转导不同哺乳动物骨髓来源间充质干细胞。生理学报,2008, 60(3): 431-436
4.孙修炼,胡志红,彭辉银.杆状病毒生态学研究进展.中国病毒学, 2006, 21(2): 200-206.
5.江云波,肖少波,方六荣等.共表达猪繁殖与呼吸综合征病毒修饰型GP5和M蛋白增强DNA疫苗免疫应答的研究.病毒学报,2007, 23(2): 130-136
6.希尼尼根,程安春,汪铭书,等.猪繁殖与呼吸综合征的研究进展.养猪, 2004 , (4) :36238.
7.张建武,庄金山,袁世山.中国部分地区“猪高热综合征”的病原学调查及高致病性猪繁殖与呼吸综合征病毒的分子流行病学研究.中国农业科学, 2008, 41(6):1822-1831
8.李兴玉.猪生殖呼吸综合症病毒分子生物学研究进展四川畜牧兽医2003,30(154):45-47
9.李真光,武华.猪繁殖与呼吸综合征病毒基因组遗传变异研究进展.动物医学进展,2009, 30:101-105
10.李祥健,张建武,吕健,余丹丹,姚火春,袁世山.猪繁殖与呼吸综合征病毒嵌合感染性克隆的构建和应用.生物工程学报, 2008, 24(9):1573-1581
11.杨金雨,仇华吉,童光志.猪繁殖与呼吸综合征疫苗研究进展.动物医学进展. 2005, 26(5): 28-31
12.陈小芳,陈元仲,邹起练等。纤维连接蛋白细胞结合区功能多肽在杆状病毒表达系统中的表达与纯化。生物技术通讯,2008,19(4): 515-517
13.郑其升,李鹏,毕志香等. PRRSV NJ-a株ORF5基因A表位的修饰与糖基化位点的突变对其DNA疫苗免疫效力的影响.生物工程学报, 2007, 23(1):33-39
14.信爱国,李华春,廖德芳等。亚洲1型口蹄疫病毒衣壳蛋白前体P1—2A基因和蛋白酶3C基因在昆虫细胞中的共表达。中国人兽共患病学报,2008,24(6):500-504
15.赵昆,刘思国,王春来等。牛抗菌肽Bac7-Bac5-βdefense串联基因在昆虫杆状病毒系统中的表达及其产物的活性分析。农业生物技术学报,2008,16(3):380-384
16.殷震,刘景华.动物病毒学[M].北京,科学出版社,1997,613-627
17.郭保清,陈章水,刘文兴等.从疑似PRRS流产胎儿中分离PRRSV的研究[J].中国畜禽传染病, 1996, 2:1-5.
18.崔保安等.猪繁殖与呼吸综合症病毒分子生物学研究进展[J].动物医学进展,2004,25(1):22-24
19. Ahrens CH, Carlson C, Rohrmann GF. Identification, sequence, and transcriptional analysis of lef-3, a gene essential for Orgyia pseudotsugata baculovirus DNA replication. ogy. 1995, 210(2):372-82.
20. Albina E, Madec F, Cariolet R, Torrison JL. Immune response and persistence of the porcinereproductive and respiratory syndrome virus in infected pigs and farm units. Vet Rec, 1994, 134:567–73.
21. Albina E. Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview. Vet Microbiol. 1997, 55:309-316.
22. Allende R, Laegreid W, Kutish G, Galeota J, Wills RW, Osorio FA. Porcine reproductive and respiratory syndrome virus: description of persistence in individual pigs upon experimental infection. J Virol, 2003, 72:10834–7.
23. Arends HM, Jehle JA. Homologous recombination between the inverted terminal repeats of defective transposon TCp3.2 causes an inversion in the genome of Cydia pomonella granulovirus. J Gen Virol. 2002, 83(Pt 7):1573-8
24. Batista L, Dee SA, Rossow KD, Deen J, Pijoan C. Assessing the duration of persistence and shedding of porcine reproductive and respiratory disease syndrome virus in a large population of breeding-age gilts. Can J Vet Res, 2002, 66:196–200.
25. Benfield DA, Christopher-Hennings J, Nelson EA, Rowland RRR, Nelson JK. Persistent fetal infection of porcine repro-ductive and respiratory syndrome virus infection. In: Proceed-ings of the 28th Ann Meet Am Assoc Swine Prac, 1997. 455–8.
26. Benfield DA, Nelson E, Collins JE, et al. Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J. Vet. Diagn. Invest. 1992, 4: 127-133.
27. Bierk MD, Dee SA, Rossow KD, Otake S, Collins JE, Molitor TW. Transmission of PRRS virus frompersistently infected sows to contact controls. Can J Vet Res 2001, 65: 261–6.
28. Bilello, J.P. et al. Transient disruption of intracellular junctions enables baculovirus entry into nondividing hepatocytes. J. Virol. 2001, 75: 9857–9871
29. Blissard GW, Rohrmann GF. Baculovirus diversity and molecular biology. Annu Rev Entomol. 1990, 35: 127-55.
30. Boublik Y., Di Bonito, P. and Jones, I. M.‘Eukaryotic virus display: Engineering the major surface glycoprotein of the Autographa californica nuclear polyhedrosis virus (AcNPV) for the presentation of foreign proteins on the virus surface’, Biotechnology, 1995, 13: 1079–1084.
31. Boyce FM, Bucher NLR. Baculovirus-mediated gene transfer into mammalian cells. Proc Natl Acad Sci USA 1996, 93: 2348–2352.
32. Broer R, Heldens JG, van Strien EA. Specificity of multiple homologous genomic regions in Spodoptera exigua nucleopolyhedrovirus DNA replication. J Gen Virol. 1998, 79 ( Pt 6): 1563-72
33. Carbonell LF, Klowden MJ, Miller LK. Baculovirus-mediated expression of bacterial genes in dipteran and mammalain cells. J Virol 1985, 56: 153–60.
34. Cary LC, Goebel M, Corsaro BG, Wang HG, Rosen E, Fraser MJ. Transposon mutagenesis of baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses. Virology. 1989, 172(1): 156-69.
35. Cavanagh D. Nidovirales: a new order comprising Coronaviridae and Arteriviridae . Arch Virol, 1997, 142(2): 629–33.
36. Cha HJ, Pham MQ, Rao G, Bentley WE. Expression of green fluorescent protein in insect larvae and its application for heterologous protein production. Biotechnol Bioeng 1997; 56: 239–247.
37. Chapple, S. D. and Jones, I. M.‘Non-polar distribution of green ?uorescent protein on the surface of Autographa californica nucleopolyhedrovirus using a heterologous membrane anchor’, J. Biotechnol., 2002, 95: 269–275.
38. Cheng T, Xu CY, Wang YB, Chen M, Wu T, Zhang J, et al. A rapid and efficient method to express target genes in mammalian cells by baculovirus. World J Gastroenterol 2004, 10: 1612–18.
39. Chris J N, Michael P M, Dkay S F. Porcine reproductive and respiratory syndrome virus Comparison: Divergent evolution on two continents [J]. J Virol, 1999,73(1): 270–280.
40. Christian Oker-Blom, Kari J. Airenne and Reingard Grabherr. Baculovirus display strategies: Emerging tools for eukaryotic libraries and gene delivery. BRIEFINGS IN FUNCTIONAL GENOMICS AND PROTEOMICS. 2003, 2: 244–253.
41. Clay WC, Condreay JP, Moore LB, Weaver SL, Watson MA, Kost TA, et al. Recombinant baculoviruses used to study estrogen receptor function in human osteosarcoma cells. Assay Drug Dev Technol 2003, 1: 801–10.
42. Collins, J. E., Benfield, D. A., Christianson, W. T., Harris, L., Hennings, J. C., Shaw, D. P., Goyal, S. M., McCullough, S., Morrison, R. B., Joo, H. S., Gorcyca, D. E., and Chladek, D. W. Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J. Vet. Diagn. Invest. 1992, 4: 117-126.
43. Condreay JP, Witherspoon SM, Clay WC, Kost TA. Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc Natl Acad Sci USA 1999, 96: 127–32.
44. Conner M, Zarley C, Hu B, Parsons S, Drabinski D, Greiner S, et al. Virus-like particles as a rotavirus subunit vaccine. J Infect Dis, 1996, 174: 88–92.
45. Conzelmann K K, Visser N, van Woesel P, et al. Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group [J]. Virology, 1993, 193(1): 329–339.
46. Cristina Fipaldini, Barbara Bellei, and Nicola La Monica. Expression of Hepatitis C Virus cDNA in Human Hepatoma Cell Line Mediated by a Hybrid Baculovirus±HCV Vector. Virology, 1999, 255: 302-311.
47. Crouch EA, Passarelli AL. Genetic requirements for homologous recombination in Autographa californica nucleopolyhedrovirus. J Virol. 2002, 76(18): 9323-34
48. Dea S, Gagnon C A, Mardassi H et al. Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus: comparison of the North American and European isolates[J]. Arch. Virol, 2000, 145: 659–688.
49. Delaney, W. E., & Isom, H. C. Hepatitis B virus replication in human HepG2 cells mediated byhepatitis B virus recombinant baculovirus. Hepatology , 1998, 28: 1134–1146
50. Delputte, P. L., Nauwynck, H. J. Porcine Arterivirus Infection of Alveolar Macrophages Is Mediated by Sialic Acid on the Virus. J. Virol. 2004, 78: 8094-8101
51. Ding-Gang Yang, Yao-Chi Chung, Yiu-Kay Lai, et al. Avian Infuenza Virus Hemagglutinin Display on Baculovirus Envelope: Cytoplasmic Domain Affects Virus Properties and Vaccine Potential. Molecular Therapy, 2007, 15: 5989–5996
52. Duffy SP, Young AM, Morin B, et al. Sequence analysis and organization of the Neodiprion abietis nucleopolyhedrovirus genome. J Virol. 2006, 80(14): 6952-63
53. Duisit G, Saleun S, Douthe S, Barsoum J, Chadeuf G, Moullier P. Baculovirus vector requires electrostatic interactions includingheparan sulfate for efficient gene transfer in mammalian cells. J Gene Med 1999, 1: 93–102.
54. Elmar A Joura, Sepp Leodolter, Mauricio Hernandez-Avila, et al. E?cacy of a quadrivalent prophylactic human papillomavirus (types 6, 11, 16, and 18) L1 virus-like-particle vaccine against high-grade vulval and vaginal lesions: a combined analysis of three randomised clinical trials. Lancet, 2007; 369: 1693–702
55. Eric JS, Janneke JMM. The molecular biology of arteriviruses [J]. J Gen Virol, 1998, 79: 961-9791.
56. Facciabene A., Aurisicchio L. and La Monica N. Baculovirus vectors elicit antigen-specific immune responses in mice. J. Virol., 2004, 78: 8663-8672
57. Fauguet CM., Mayo MA., Maniloff J., et al. Virus Taxonomy: the Eighth Report of the International Committee on Taxonomy of Viruses. 2005, New Youk
58. Fipaldini C, Bellei B, La Monica N. Expression of hepatitis C virus cDNA in human hepatoma cell line mediated by a hybrid baculovirus-HCV vector. Virology, 1999; 255: 302–11.
59. Fraser MJ, Ciszczon T, Elick T, et al. Precise excision of TTAA-specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera. Insect Mol Biol. 1996, 5(2): 141-51.
60. Free In PMC, Virological and immunological responses to Porcine reproductive and respiratory syndrome virus In a large population of gilts. Can J Vet Res.,2004, 68(4): 267-73.
61. Friesen PD, Nissen MS. Gene organization and transcription of TED, a lepidopteran retrotransposon integrated within the baculovirus genome. Mol Cell Biol. 1990, 10(6): 3067-77
62. Friesen PD, Rice WC, Miller DW, Miller LK. Bidirectional transcription from a solo long terminal repeat of the retrotransposon TED: symmetrical RNA start sites. Mol Cell Biol. 1986, 6(5): 1599-607
63. Gerhardt E, Bruss V. Phenotypic mixing of rodent but not avian hepadnavirus surface proteins into human hepatitis B virus particles. J Virol 1995; 69: 1201–8.
64. Gronowski, A.M. et al. Baculovirus stimulates antiviral effects in mammalian cells. J. Virol. 1999, 73: 9944–9951
65. Guarino LA, Gonzalez MA, Summers MD. Complete Sequence and Enhancer Function of the Homologous DNA Regions of Autographa californica Nuclear Polyhedrosis Virus. J Virol. 1986,60(1): 224-229.
66. Guarino LA, Jin. J and Dong W. Guanylyltransferase activity of the LEF-4 subunit of baculovirus RNA polymerase. J Virol. 1998, 72(12): 10003-10.
67. HAIYING YU, OLAF ISKEN, CLAUS W. GRASSMANN, et al. A Stem-Loop Motif Formed by the Immediate 59 Terminus of the Bovine Viral Diarrhea Virus Genome Modulates Translation as well as Replication of the Viral RNA. J. VIROL., 2000, 74(13): 5825–5835
68. Hajek KL, Friesen PD. Proteolytic processing and assembly of gag and gag-pol proteins of TED, a baculovirus-associated retrotransposon of the gypsy family. J Virol. 1998 ,72(11): 8718-24
69. Hajós JP, Pijnenburg J, Usmany M, et al. High frequency recombination between homologous baculoviruses in cell culture. Arch Virol. 2000, 145(1): 159-64.
70. Harrison RL, Bonning BC. Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses. J Gen Virol. 2003, 84(Pt 7): 1827-42
71. Hefferon KL, Oomens AG, Monsma SA, Finnerty CM, Blissard GW. Host cell receptor binding by baculovirus GP64 and kinetics of virion entry. Virology. 1999, 258(2): 455-68
72. Herniou EA, Luque T, Chen X, Vlak JM, et al. Use of whole genome sequence data to infer baculovirus phylogeny. J Virol., 2001, 75(17):8117-26
73. Herniou EA, Olszewski JA, O'Reilly DR, Cory JS. Ancient coevolution of baculoviruses and their insect hosts. J virol., 2004, 78: 3244-3251.
74. Hideki Tani, Chang Kwang Limn,Chan Choo Yap, et al. In Vitro and In Vivo Gene Delivery by Recombinant Baculoviruses. J Virol. 2003, 77(18): 9799–9808
75. Ho Y, Lin PH, Liu CYY, Lee SP, Chao YC. Assembly of human severe acute respiratory syndrome coronavirus-like particles. Biochem Biophys Res Commun, 2004, 318: 833–8.
76. Ho Y, Lo HR, Lee TC, Wu CP, Chao YC. Enhancement of correct protein folding in vivo by a non-lytic baculovirus. Biochem J. 2004, 382: 695–702.
77. Ho YC, Chen HC, Wang KC, Hu YC. Highly efficient baculovirus-mediated gene transfer into rat chondrocytes. Biotechnol Bioeng, 2004, 88: 643–51.
78. Hofmann C, Sandig V, Jennings G, Rudolph M, Schlag P, Strauss M. Efficient gene-transfer into human hepatocytes by baculovirus vectors. Proc Natl Acad Sci USA, 1995, 92: 10099–103.
79. Hollister JR, Shaper JH, Jarvis DL. Stable expression of mammalian beta 1,4-galactosyltransferase extends the N-glycosylation pathway in insect cells. Glycobiology 1998, 8: 473–80.
80. Horter DC, Pogranichiny RM, Chang CC, Evans RB, Yoon KJ, Zimmerman JJ. Characterization of the carrier state in porcine reproductive and respiratory syndrome virus infection. Vet Microbiol 2002, 86: 213–28.
81. HU Yu-chen. Baculovirus as a highly efficient expression vector in insect and mammalian cells. Acta Pharmacologica Sinica, 2005, 26 (4): 405–416
82. Hua-Ji Qiu, Zhi-Jun Tian, Guang-Zhi Tong, et al. Protective immunity induced by a recombinant pseudorabies virus expressing the GP5 of porcine reproductive and respiratory syndrome virus in piglets. Veterinary Immunology and Immunopathology, 2005, 106: 309–319
83. Jarvis DL, Aumiller JJ, Hollister JR, Kawar ZS, Pilon J, Vadaie N. Analysis and manipulation of the protein N-glycosylation pathway in the baculovirus-insect cell system. Glycobiology, 2004, 14: 1059–60.
84. Jarvis DL, Fleming J, Kovacs GR, Summers MD, Guarino LA. Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells. Biotechnology 1990, 8: 950–55.
85. Jarvis DL. Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production. Virology, 2003, 310: 1–7.
86. Jehle JA, Blissard GW, Bonning BC, et al. On the classification and nomenclature of baculoviruses: a proposal for revision. Arch Virol. 2006, 151(7): 1257-66.
87. Jehle JA, Fritsch E, Huber J. Intra-specific and inter-specific recombination of tortricid-specific granuloviruses during co-infection in insect larvae. Arch Virol. 2003, 148(7): 1317-33.
88. Jehle JA. The expansion of a hypervariable, non-hr ori-like region in the genome of Cryptophlebia leucotreta granulovirus provides in vivo evidence for the utilization of baculovirus non-hr oris during replication. J Gen Virol. 2002, 83(Pt 8): 2025-34.
89. Jehle JA. The mosaic structure of the polyhedrin gene of the Autographa californica nucleopolyhedrovirus (AcMNPV). Virus Genes, 2004, 29(1): 5-8
90. Jenkinson S, McCoy DC, Kerner SA, Ferris RG, Lawrence WK, Clay WC, et al. Development of a novel high-throughput surrogate assay to measure HIV envelope/CCR5/CD4-mediated viral/cell fusion using BacMam baculovirus technology. J Biomol Screen, 2003, 8: 463–70.
91. Jenny G. Cho, Scott A. Dee. Porcine reproductive and respiratory syndrome virus. Theriogenology, 2006, 66: 655–662
92. John J. Treanor; Gilbert M. Schiff; Frederick G. Hayden; et al. Safety and Immunogenicity of a Baculovirus-Expressed Hemagglutinin Influenza Vaccine: A Randomized Controlled Trial. JAMA, 2007, 297(14): 1577-1582
93. Joo H S ,Park B K,Dee S A ,et al . Indirect fluorescent IgM antibody response ofbigs infected with porcine reproductive and respiratory syndrome virus . Vet Microbiol, 1997, 55: 303-307.
94. Kamita SG, Maeda S, Hammock BD. High-frequency homologous recombination between baculoviruses involves DNA replication. J Virol. 2003, 77(24): 13053-61
95. Kazuhiro Okano, Adam L. Vanarsdall, Victor S. Mikhailov, et al. Conserved molecular systems of the Baculoviridae. Virology, 2006, 344: 77– 87
96. Keffaber KK. Reproductive failure of unknown etiology. Am. Assoc. Swine Prac. Newsl, 1989,1: 1-9.
97. Kitts PA. and Possee RD. A method for producing recombinant baculovirus expression vectors at high frequency. Biotechniques, 1993, 14: 810-817.
98. Kitts PA, Ayres MD and Possee RD. Linearization of baculovirus DNA enhangces the recovery of recombinant virus expression vectors. Nucleic Acids Res., 1990, 18: 5667-5672.
99. Kost TA, Condreay JP. Recombinant baculoviruses as mammalian cell gene delivery vectors.Trends Biotechnol, 2002, 20: 173–80.
100. Kristensen CS, Botner A eatl. Experimental airborne transmission of PRRS virus[J]. Vet Microbiol. 2004, 99(3-4): 197-202
101. Lange M, Wang H, Zhihong H, et al. Toward a molecular indentification and classification system of lepidopteran-specific baculovirus. Virology, 2004, 325: 36-47.
102. Laprise M-H, Grondin F, Dubois CM. Enhanced TGFβ1 maturation in high five cells coinfected with recombinant baculovirus encoding the convertase furin/pace: improved technology for the production of recombinant proproteins in insect cells. Biotechnol Bioeng, 1998, 58: 85–91.
103. Lee C, Yoo D. The small envelope protein of porcine reproductive and respiratory syndrome virus possesses ion channel protein-like properties [J]. Virology, 2006, 355(1): 30–43.
104. Lee C,Rogan D,Erickson L,Zhang J ,Yoo D. Chatacterization of the porcine reproductive and respiratory syndrome virus glycoprotein5(GP5) in stably expressing cells. [J] Virus Res, 2004, 104(1): 33-8
105. Lee H, Krell PJ. Reiterated DNA fragments in defective genomes of Autographa californica nuclear polyhedrosis virus are competent for AcMNPV-dependent DNA replication. Virology, 1994, 202(1): 418-29
106. Lee HY, Krell PJ. Generation and analysis of defective genomes of Autographa californica nuclear polyhedrosis virus. J Virol. 1992, 66(7): 4339-47
107. Lemke C D ,Haynes J S ,Spaete R ,et al. Lymphoid hyperplasia resulting in immune dysregulation is caused by Porcine reproductive and respiratory syndrome virus infection in neonatal pigs . J Immunol, 2004, 172: 1916-1925.
108. Lihoradova OA, Bachurina EY, Azimova SS. Biosynthesis of the recombinant middle surface antigen of the human hepatitis B virus in silkworm larvae. Mol Biol, 2004, 38: 603–7.
109. Lo HR, Chou CC, Wu TY, Yuen JP, Chao YC. Novel baculovirus DNA elements strongly stimulate activities of exogenous and endogenous promoters. J Biol Chem 2002, 277: 5256–64.
110. Lopez OJ., Osorio FA. Role of neutralizing antibodies in PRRSV protective immunity. Veterinary Immunology and Immunopathology, 2004, 102: 155–163
111. Lu A, Miller LK. The roles of eighteen baculovirus late expression factor genes in transcription and DNA replication. J Virol. 1995, 69(2): 975-82.
112. Lucifora J, Durantel D, Belloni L, et al. Initiation of hepatitis B virus genome replication and production of infectious virus following delivery in HepG2 cells by novel recombinant baculovirus vector. J. Gen. Virol. 2008, 89: 1819–1828
113. Luckow VA, Lee SC, Barry GF, et al. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in E. coli. J. Virol., 1993, 67: 4566-4579.
114. Maeda S, Kamita SG, Kondo A. Host range expansion of Autographa californica nuclear polyhedrosis virus (NPV) following recombination of a 0.6-kilobase-pair DNA fragment originating from Bombyx mori NPV. J Virol. 1993, 67(10): 6234-8
115. Marcelo de Lima, Asit K. Pattnaik, Eduardo F. Flores, et al. Serologic marker candidates identified among B-cell linear epitopes of Nsp2 and structural proteins of a North American strain of porcine reproductive and respiratory syndrome virus. Virology, 2006, 353: 410–421
116. Marcelo de Lima, Byungjoon Kwon, Israrul H. Ansari, et al. Development of a porcine reproductive and respiratory syndrome virus differentiable (DIVA) strain through deletion of specific immunodominant epitopes. Vaccine, 2008, 26: 3594–3600
117. McDougal VV, Guarino LA. DNA and ATP binding activities of the baculovirus DNA helicase P143. J Virol. 2001, 75(15): 7206-9.
118. Mengeling, WL., Lager, KM., Vorwald, AC. Clinical consequences of exposing pregnant gilts to strains of porcine reproductive and respiratory syndrome (PRRS) virus isolated from field cases of“atypical”PRRS. Am. J. Vet. Res. 1998, 59: 1540-1544.
119. Merrihew RV, Clay WC, Condreay JP, Witherspoon SM, Dallas WS, Kost TA. Chromosomal integration of transduced recombinant baculovirus DNA in mammalian cells. J Virol, 2001, 75: 903–9.
120. Meulenberg JJ, Hulst MM, de Meijer EJ, et al. Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS) is related to LDV and EAV [J]. Virology, 1993, 192(1): 62–72.
121. MEULENBERG JJM., JNA BOS-DE RUIJTER, R. VAN DE GRAAF, et al. Infectious Transcripts from Cloned Genome-Length cDNA of Porcine Reproductive and Respiratory Syndrome Virus. J. VIROL., 1998, 72(1): 380–387
122. Mikhailov VS. Helix-destabilizing properties of the baculovirus single-stranded DNA-binding protein (LEF-3). Virology. 2000, 270(1): 180-9
123. Monique M. van Oers and Just M. Vlak. Baculovirus Genomics. Current Drug Targets, 2007, 8: 1051-1068
124. Mortola E, Roy P. Efficient assembly and release of SARS coronavirus-like particles by a heterologous expression system. FEBS Lett, 2004, 576: 174–8.
125. Mottershead DG., Alfthan K, Ojala K. et al.‘Baculoviral display of functional scFv and synthetic IgG-binding domains’, Biochem. Biophys. Res. Commun., 2000, 275: 84–90.
126. Murtaugh MP, Elam MR, Kakach LT. Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus [J]. Arch Biol, 1995, 140: 1451–60.
127. Narinder Pal, Sandhya Boyapalle, Randy Beckett, et al. A Baculovirus-Expressed Dicistrovirus That Is Infectious to Aphids. J VIROL, 2007, 81(17): 9339–9345
128. Narinder Pal, Sandhya Boyapalle, Randy Beckett, et al. A Baculovirus-Expressed Dicistrovirus That Is Infectious to Aphids. J. VIROL. 2007, 81(17): 9339–9345
129. Nielsen J, Botner A, Bille Hansen V, et al. Experimental inoculation of late term pregnant sows with a field isolate of porcine reproductive and respiratory syndrome vaccine-derived virus . Vet Microbiol, 2002, 84: 1213.
130. Ohkawa T, Rowe AR and Volkman LE.‘Identification of six Autographa californica multicapsidnucleopolyhedrovirus early genes that mediate nuclear localization of G-actin’, J. Virol., 2002, 76: 2281–12289.
131. Ojala K, Mottershead DG., Suokko A. and Oker-Blom C.‘Specific binding of baculoviruses displaying gp64 fusion proteins to mammalian cells’. Biochem. Biophys. Res. Commun., 2001, 284: 777–784.
132. Okano K, Vanarsdall AL, Mikhailov VS, et al. Conserved molecular systems of the Baculoviridae[J]. Virology, 2006, 344: 77-87.
133. Olson VA, Wetter JA, Friesen PD. The highly conserved basic domain I of baculovirus IE1 is required for hr enhancer DNA binding and hr-dependent transactivation. J Virol. 2003, 77(10): 5668-77.
134. Olszewski J, Miller LK. A role for baculovirus GP41 in budded virus production. Virology. 1997, 233(2): 292-301
135. Oomens AG, Monsma SA, Blissard GW. The baculovirus GP64 envelope fusion protein: synthesis, oligomerization, and processing. Virology. 1995, 209(2): 592-603.
136. Oomens AG. and Blissard GW.‘Requirement for gp64 to drive efficient budding of Autographa californica multicapsid nucleopolyhedrovirus’, Virology, 1999, 254: 297–314.
137. Oppenheimer DI, Volkman LE. Evidence for rolling circle replication of Autographa californica M nucleopolyhedrovirus genomic DNA. Arch Virol. 1997, 142(10): 2107-13.
138. Osorio FA, Zuckermann F, Wills R, Meier W, Christian S, Galeota J, Doster A. PRRSV: comparison of commercial vaccines in their ability to induce protection against current PRRSV strains of high virulence. Allen D. Leman Swine Conference. 1998, 25: 176-182
139. Otake S, Dee SA, Rossow KD, Deen J, Joo HS, Molitor TW, et al. Transmission of porcine reproductive and respiratory syndrome virus by fomites (boots and coveralls). Swine Health Prod ,2002, 10: 59–65.
140. Palhan VB, Sumathy S, Gopinathan KP. Baculovirus mediated high-level expression of luciferase in silkworm cells and larvae. Biotechniques, 1995, 19: 97–104.
141.Patterson RM, Selkirk JK, Merrick BA. Baculovirus and insect cell gene expression-review of baculovirus biotechnology. Environ Health Perspect, 1995, 103: 756–9.
142. Pearson MN, Rohrmann GF. Lymantria dispar nuclear polyhedrosis virus homologous regions: characterization of their ability to function as replication origins. J Virol. 1995, 69(1): 213-21
143. Pearson MN, Russell RL, Rohrmann GF, Beaudreau GS. p39, a major baculovirus structural protein: immunocytochemical characterization and genetic location. Virology. 1988, 167(2): 407-13
144. Pijlman GP, Dortmans JC, Vermeesch AM, et al. Pivotal role of the non-hr origin of DNA replication in the genesis of defective interfering baculoviruses. J Virol. 2002, 76(11): 5605-11.
145. Pijlman GP, van den Born E, Martens DE. Autographa californica baculoviruses with large genomic deletions are rapidly generated in infected insect cells. Virology. 2001, 283(1): 132-8.
146. Pijlman GP, van Schijndel JE, and Vlak JM. Spontaneous excision of BAC vector sequences from bacmid-derived baculovirus expressio vectors upon passage in insect cells. J. Gen. Virol., 2003, 84:2669-2678.
147. Pirzadeh B, Dea S .Immune response in pigs vaccinated with plasmid DNA encoding ORF5 of porcine reproductive and respiratory syndrome virus. Journal of General Virology, 79: 989-999
148. Plagemann PG, Rowland RR. Polyclonal hypergammaglobulinemia and formation of hydrophobic immune complexes in porcine reproductive and respiratory syndrome virus-infected and uninfected pigs [J] Viral Immunol. 2005, 18(1): 138-47
149. Plana Duran ,Collins J E ,Mengling WL ,et al . Efficacy of an inactivated vaccine for Prevention of reproductive failure induced by porcine reproductive and respiratory syndrome virus .Vet Microbiol , 1997 , 55: 361-370.
150. Planaduran J,Climent I,sarraseca J, et al. Baculovrius expression of proteins of porcine reproductive and respiratory syndrome virus st rain Olot/ 91 Involvement of ORF3 and ORF5 proteins in protection . Virus Genes ,1997 ,14: 19229.
151. Raju TS, Lerner L, O’Connor JV. Glycopinion: biological significance and methods for the analysis of complex carbohydrates of recombinant glycoproteins. Biotechnol Appl Biochem., 1996, 24: 191–4
152. Robert Strauss, Andreas Huser, Shaoheng Ni, et al. Baculovirus-based Vaccination Vectors Allow for Efficient Induction of Immune Responses Against Plasmodium falciparum Circumsporozoite Protein. Molecular Therapy, 2007, 15: 193–202
153. Rohrmann GF. Polyhedrin structure. J Gen Virol. 1986, 67 ( Pt 8): 1499-513.
154. Russell RL, Pearson MN and Rohrmann GF.‘Immunoelectron microscopic examination of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus-infected Lymantria dispar cells: Time course and localization of major polyhedron- associated proteins’, J. Gen. Virol., 1991, 72: 275–283.
155. Sarkis C, Serguera C, Petres S, Buchet D, Ridet JL, Edelman L, et al. Efficient transduction of neural cells in vitro and in vivo by a baculovirus-derived vector. Proc Natl Acad Sci USA, 2000, 97: 14638–43.
156. Shoji I, Aizaki H, Tani H, Ishii K, Chiba T, Saito I, et al. Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors. J Gen Virol, 1997, 78: 2657–64.
157. Siao-Kun Wan Welch, Rika Jolie, Douglas S. Pearce, et al. Construction and evaluation of genetically engineered replication-defective porcine reproductive and respiratory syndrome virus vaccine candidates. Veterinary Immunology and Immunopathology, 2004, 102: 277–290
158. Smith G. P. Filamentous fusion phage: novel expression vector that display cloned antigens on the virion surface[J]. Science, 1985, 228: 1315-1317.
159. Smith GE., Summers MD. and Fraser MJ. Pruduction of human beta interferon in insect cells infected with a baculovirus expression vector. Mol. Cell. Biol., 1983, 3: 2156-2165
160. Snijder E J and Meulenberg J J M. The molecular biology of arteriviruses[J]. J Gen Virol, 1998, 79: 961-979.
161. Spenger, A., Grabherr, R., Tollner, L. et al.‘Altering the surface properties of baculovirusAutographa californica NPV by insertional mutagenesis of the envelope protein gp64’, Eur. J. Biochem. 2002, 269: 4458–4467.
162. Steinhaus EA. Duration of infectivity of the virus of silkworm jaundice. Science, 1954, 120: 186-187.
163. Tami C, Farber M, Palma EL. and Taboga O.‘Presentation of antigenic sites from foot-and-mouth disease virus on the surface of baculovirus and in the membrane of infected cells’, Arch. Virol., 2000, 145: 1815–1828.
164. Tani, H. et al. Characterization of cell surface determinants important for baculovirus infection. Virology, 2001, 279: 343–353
165. Thiem SM, Miller LK. Identification, sequence, and transcriptional mapping of the major capsid protein gene of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol. 1989 , 63(5): 2008-18.
166. Thomas A Kost, J Patrick Condreay and Donald L Jarvis. Baculovirus an versatile vectors for protein expression in insect and mammalianm cells. Nature Biotechnology, 2005, 23(5): 567-575
167. Thomas A. Kost and J. Patrick Condreay. Baculovirus Expression Vectors for Insect and Mammalian Cells. Current Drug Targets, 2007, 8: 1126-1131
168. Thomas A. Kost and J. Patrick Condreay. Recombinant baculoviruses as mammalian cell gene-delivery vectors. TRENDS in Biotechnology, 2002, 20 (4): 173-180
169. Tian K, Yu X, Zhao T, et al. Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark [J]. PLoS ONE, 2007,2(6): 526.
170. Tjia, ST., G. M. zu Altenschildesche, and W. Doer?er. Autographa californica nuclear polyhedrosis virus (AcNPV) DNA does not persist in mass cultures of mammalian cells. Virology, 1983, 125: 107–117.
171. Todd JW, Passarelli AL, Lu A, Miller LK. Factors regulating baculovirus late and very late gene expression in transient-expression assays. J Virol. 1996, 70(4): 2307-17
172. Urabe M., Ding C. and Kotin RM. Insect cells as a factory to produce adeno-associated virus type 2 vectors , 2002, Hum. Gene Ther., 13(16): 1935-1943.
173. van Loo, N.D. et al. Baculovirus infection of non-dividing mammalian cells: mechanisms of entry and nuclear transport of capsids. J. Virol. 2001, 75: 961–970
174. van Oers MM, Vlak JM. Baculovirus genomics. Curr Drug Targets. 2007, 8(10): 1051-68.
175. Vanarsdall AL, Okano K, Rohrmann GF. Characterization of the replication of a baculovirus mutant lacking the DNA polymerase gene. Virology, 2005, 331(1): 175-80
176. Venkaiah B, Viswanathan P, Habib S, Hasnain SE. An additional copy of the homologous region (hr1) sequence in the Autographa californica multinucleocapsid polyhedrosis virus genome promotes hyperexpression of foreign genes. Biochemistry, 2004, 43: 8143–51.
177. Viswanathan P, Venkaiah B, Kumar MS, et al. The homologous region sequence (hr1) of Autographa californica multinucleocapsid polyhedrosis virus can enhance transcription from non-baculoviral promoters in mammalian cells. J Biol Chem, 2003, 278: 52 564–71.
178. Vlak J M., Schoutten A., Usmany M., et al. Expression of cauliflower mosaic virus geneⅠusing a baculovirus based upon the p10 gene and a novel selection method. Virology, 1990, 179: 312-320.
179. Volkman LE, Goldsmith PA. In vitro study of Autographa californica nuclear polyhedrosis virus interaction with nontarget vertebrate host cells. Appl Environ Microbiol, 1983, 45: 1085–1093.
180. Wagle M, Jesuthasan S. Baculovirus-mediated gene expression in zebrafish. Marine Biotechnol 2003, 5: 58–63.
181. Wang KC, Wu JC, Chung YC, et al. Baculovirus as a highly efficient gene delivery vector for the expression of hepatitis delta virus antigens in mammalian cells. Biotechnol Bioeng, 2005, 89: 464-73.
182. Wang Y, Liang Y, Han J, et al. Attenuation of porcine reproductive and respiratory syndrome virus strain MN184 using chimeric construction with vaccine sequence. Virology, 2008, 371(2): 418-29.
183. Wensvoort G, Terpstra C, Pol JM, et al. Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Veterinary Quarterly, 1991, 13: 121-130.
184. Wills RW, Zimmerman JJ, Yoon KJ, et al. Porcine reproductive and respiratory syndrome virus: a persistent infection. Vet Microbiol, 1997, 55: 231–40.
185. Wissink EHJ, MV Kroese, HAR. van Wijk, et al. Envelope Protein Requirements for the Assembly of Infectious Virions of Porcine Reproductive and Respiratory Syndrome Virus. J. VIROL., 2005, 79(19): 12495–12506
186. Wootton S, Yoo D, Rogan D. Full-length sequence of a Canadian porcine reproductive and respiratory syndrome virus (PRRSV) isolate. Arch Virol, 2000, 145(11): 2297–2323.
187. Xiangjie Yao, Qingxia Han , Jianhua Song , et al. Baculovirus Mediated Production of Infectious Hepatitis C Virus in Human Hepatoma Cells Stably Expressing T7 RNA Polymerase. Mol Biotechnol, 2008, 40: 186–194
188. Yu-chen HU Baculovirus as a highly efficient expression vector in insect and mammalian cells. Acta Pharmacologica Sinica, 2005, 26 (4): 405–416
189. Ziebuhr J, Snijder E J and Gorbalenya A E. Virus-encoded proteinases and proteolytic processing in the Nidovirales[J]. J Gen Virol, 2000, 81: 853-879