p16和Kiss-1在大肠癌中的表达及意义
摘要
目的：探讨肿瘤转移抑制基因p16和肿瘤抑制基因Kiss-1mRNA和蛋白在大肠癌中的表达及其生物学特性的关系。
方法：选择50例大肠癌和10例正常大肠黏膜组织,采用逆转录聚合酶链反应(RT-PCR)检测p16和Kiss-1基因mRNA,免疫组织化学染色采用Elivison二步法检测p16和Kiss-1蛋白的表达。
结果：1. RT-PCR结果：在大肠癌组中P16和Kiss-1mRNA表达量低于正常黏膜组(P<0.05),其表达量与大肠癌的Broders分级和Dukes分期相关；有淋巴结转移组中P16和Kiss-1mRNA表达量明显低于无转移组(P<0.05)。P16和Kiss-1mRNA在大肠癌中的表达呈正相关。2.免疫组化结果：在大肠癌与正常大肠黏组中p16蛋白表达率分别为48%和100%,Kiss-1蛋白的表达率各为56%和100%,p16和Kiss-1蛋白随大肠癌的分级、分期的增高其表达率逐渐下降；有淋巴结转移组中p16和Kiss-1蛋白的表达明显低于无转移组(P<0.05)。3.P16和Kiss-1mRNA和其蛋白的表达率与大肠癌患者的年龄、性别和大体类型无明显差异。
结论：1.P16和Kiss-1基因在大肠癌的发生、发展中发挥重要的作用。2.P16和Kiss-1mRNA及其蛋白表达缺失与大肠癌的浸润和转移有关。3.联合检测P16和Kiss-1的基因可预测大肠癌患者的预后。
Objective:To explore the relationship between expression of tumor suppressor metastasis gene p16 and tumor suppressor gene Kiss-1 and their biological characteristics in colorectal cancer.
Methods:The expression of p16 and Kiss-1 mRNA was determined by RT-PCR method in 50 cases of colorectal cancer and 10 adjacent normal mucosa.The expression of p16 and Kiss-1 proteins was determined by immunohistochemical Elivison methods.
Results:1.RT-PCR results:The expression quantity of p16 and Kiss-1 mRNA was significantly lower in colorectal cancer than that in adjacent normal mucosa (P<0.05).The expression quantity of p16 and Kiss-1 mRNA were correlated with the Broders grading and Dukes staging of colorectal cancer, and it was significantly correlated with lymph node metastasis (P<0.05).There was positive correlation between p16 and Kiss-1 mRNA expression.2.Immunohistochemistry results:The expression rates of p16 protein were 48% and 100% in colorectal cancer and adjacent normal mucosa, and Kiss-1 protein was 56% and 100%, respectively.The expression rate of p16 and Kiss-1 protein was correlated with the grading and staging of colorectal cancer. The expression of p16 and Kiss-1 protein was significantly correlated with lymph node metastasis (P<0.05).3.The expression of p16 and Kiss-1 mRNA and protein was not correlated with age, gender and gross type.
Conclusions:1.The p16 and Kiss-1 genes plays an important role in colorectal cancer genesis.2. The lower expression of p16 and Kiss-1 mRNA protein was correlated with infiltration and metastasis in colorectal cancer.3. Combination determine of p16 and Kiss-1 genes might be useful in indicating the prognosist in patients with colorectal cancer.
方法：选择50例大肠癌和10例正常大肠黏膜组织,采用逆转录聚合酶链反应(RT-PCR)检测p16和Kiss-1基因mRNA,免疫组织化学染色采用Elivison二步法检测p16和Kiss-1蛋白的表达。
结果：1. RT-PCR结果：在大肠癌组中P16和Kiss-1mRNA表达量低于正常黏膜组(P<0.05),其表达量与大肠癌的Broders分级和Dukes分期相关；有淋巴结转移组中P16和Kiss-1mRNA表达量明显低于无转移组(P<0.05)。P16和Kiss-1mRNA在大肠癌中的表达呈正相关。2.免疫组化结果：在大肠癌与正常大肠黏组中p16蛋白表达率分别为48%和100%,Kiss-1蛋白的表达率各为56%和100%,p16和Kiss-1蛋白随大肠癌的分级、分期的增高其表达率逐渐下降；有淋巴结转移组中p16和Kiss-1蛋白的表达明显低于无转移组(P<0.05)。3.P16和Kiss-1mRNA和其蛋白的表达率与大肠癌患者的年龄、性别和大体类型无明显差异。
结论：1.P16和Kiss-1基因在大肠癌的发生、发展中发挥重要的作用。2.P16和Kiss-1mRNA及其蛋白表达缺失与大肠癌的浸润和转移有关。3.联合检测P16和Kiss-1的基因可预测大肠癌患者的预后。
Objective:To explore the relationship between expression of tumor suppressor metastasis gene p16 and tumor suppressor gene Kiss-1 and their biological characteristics in colorectal cancer.
Methods:The expression of p16 and Kiss-1 mRNA was determined by RT-PCR method in 50 cases of colorectal cancer and 10 adjacent normal mucosa.The expression of p16 and Kiss-1 proteins was determined by immunohistochemical Elivison methods.
Results:1.RT-PCR results:The expression quantity of p16 and Kiss-1 mRNA was significantly lower in colorectal cancer than that in adjacent normal mucosa (P<0.05).The expression quantity of p16 and Kiss-1 mRNA were correlated with the Broders grading and Dukes staging of colorectal cancer, and it was significantly correlated with lymph node metastasis (P<0.05).There was positive correlation between p16 and Kiss-1 mRNA expression.2.Immunohistochemistry results:The expression rates of p16 protein were 48% and 100% in colorectal cancer and adjacent normal mucosa, and Kiss-1 protein was 56% and 100%, respectively.The expression rate of p16 and Kiss-1 protein was correlated with the grading and staging of colorectal cancer. The expression of p16 and Kiss-1 protein was significantly correlated with lymph node metastasis (P<0.05).3.The expression of p16 and Kiss-1 mRNA and protein was not correlated with age, gender and gross type.
Conclusions:1.The p16 and Kiss-1 genes plays an important role in colorectal cancer genesis.2. The lower expression of p16 and Kiss-1 mRNA protein was correlated with infiltration and metastasis in colorectal cancer.3. Combination determine of p16 and Kiss-1 genes might be useful in indicating the prognosist in patients with colorectal cancer.
引文
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[3]Noborl T,Miura K,Wu DJ,et al. Deletions of the cyclin-dependent kinase-4 inhibitor in multiple human cancers [J].Nature,1994,368(6473):753-756.
[4]Kamb A,Gruis NA,Feldhaus JW,et al. A cell cycle regulator potentially involved in genesis of many tumor types [J].Science,1994,264(15):436-460.
[5]StoneS, Jiang P, Dayananth P, et al. Complex structure and regulation of p16 (MTS1) locus [J]. Cancer Res,1995,55(14):2988-2994.
[6]Mao L,Merlo A,Bedi G,et, al..A novel p16INK4 transcript[J].Cancer Res,1995, 55(14):2995-2996.
[7]LiYan, Nichols MA, Shay JW, et al. Transcriptional repression of the D-typecyclin-dependent kinase inhibitor p16 by the retinoblastoma susceptibility gene productpRb. Cancer Res,1994,54(23):6078.
[8]Tam S, Shay J, Pagano M. Differential expression and cell cycle regulation of thecyclin-dependent kinase 4 inhibitor p16INK4a.Cancer Res,1994,54(22):5816.
[9]James G, Herman, Adrian M, et al. Inactivation of the CDKN2 p16/MTS1 gene is frequently associated with aberrant DNA methylation in all common human cancers. Cancer Res,1995,55(15):4525.
[10]Merlo A, Herman JG, Mao L.5'-CpG island methylation is associated with transcriptional silencing of the tumor suppressor p16/CDKN2/MTS1 in human cancer. Nat Med,1995,1(124):686.
[11]Tate PH, Bird AP. Effects of DNA methylation of DNA-banding proteins and gene expression. Curr Opin Genet Dev,1993,3(87):226.
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