芽孢杆菌的分类鉴定及其相关属的分类系统演变研究
|
摘要
采用稀释涂布法从我国15个省15份土样标本中分离出322株芽孢杆菌。通过表型特征、脂肪酸成分、ITS测序等手段将322株菌鉴定为3个属,芽孢杆菌属Bacillus 11个种,类芽孢杆菌属Paenibacillus属2个种,短短芽孢杆菌属Brevibacillus属2个种。
本文对芽孢杆菌的生物学特性进行了研究,观察了枯草芽孢杆菌、简单芽孢杆菌、多粘类芽孢杆菌的细胞形态,并对几种常见芽孢杆菌的菌落形态进行了描述,也对枯草芽孢杆菌的生长特性做了相关的研究。根据鉴定结果,统计分析了我国部分省芽孢杆菌的多样性,研究结果表明每个样点分离到芽孢杆菌的数量差异很大,每个采样点的优势种群的种类和数量也有很大差异。
15个采样点土样分离到的芽孢杆菌丰富度在2-8之间。其中,青海采样点的芽孢杆菌丰富度最小,只分离到2种;新疆、内蒙古和宁夏芽孢杆菌的丰富度最大,为8。新疆采样点的丰富度和多样性指数最高,其均匀度指数较高,生态优势度指数较低。西藏的芽孢杆菌丰富度较低,多样性指数和均匀度指数最低,生态优势度指数最高。因此物种多样性不仅与物种的丰富度有关,还可能与物种间数量差异程度有一定关系。
本文还初步研究了芽孢杆菌对香蕉枯萎病病原菌和青枯雷尔氏菌的拮抗作用,筛选到22株对香蕉枯萎病病原菌有拮抗作用,15株对青枯雷尔氏菌有拮抗作用。从空间分布来看,具有拮抗作用的菌株大部分是从北方各省分离到的。
最后,本文讲述了芽孢杆菌属属水平上的分类变化现状,叙述了芽孢杆菌属相关属的分类依据及模式种特征。截止2008年12月份,IJSEM收录的芽孢杆菌属相关属有41个,芽孢杆菌属内已知种有112个。本论文还根据IJSEM上发表的文章对112个芽孢杆菌种的特征进行整理,记录了每个种的特征、16S rRNA序列号和菌株编号。
Based on the 15 soil samples collected from 15 provinces of China, 322 Bacillus strains were isolated by spread plate method. The 322 Bacillus strains were identified through morphological characteristics, physical and biological characteristics, fatty acid and ITS sequence. There were 11 species of Bacillus, 2 species of Panebacillus and 2 species of Brevibacillus had been recognized.
The biological characteristcis of Bacillus were studied in the paper. The cell morphologies of Bacillus subtilis, Bacillus simplex and Bacillus polymyxa, the colonial morphologies of Bacillus spp., and growth characteristic of Bacillus subtilis were studied. According to the preliminary result, the diversity of Bacillus collected from 15 provinces of China , showed that the quantity, varieties and as well as dominant species of Bacillus were different from each province.
The species richness of Bacillus of 15 soil samples collected from 15 provinces of China was analyzed. The results showed that the species richness of Bacillus from Qinghai, with 2 species was the lowest and the species richness of Bacillus from Xinjiang, Iner Mongolia and Ningxia, with 8 species were the highest. The diversity index and evenness index of Bacillus from Xinjiang were higher than those from Tibet, while the ecological dominant index of Bacillus from Xinjiang were lower than that from Tibet. It indicated that the species diversity was not only related to speceis richness, but also to individual quantity difference.
The antagonistic action of Bacillus to Ralstonia solanacearum and Fusarium oxysporum was preliminarily studied. The result showed that 22 strains had the antagonistic action to Fusarium oxysporum and 15 strains had the antagonistic action Fusarium oxysporum and Ralstonia solanacearum, which were mostly isolated from the soil samples collected from the northern provinces of China.
Finally, the systematic state of Bacillus, the taxonomic evidence of the related genera of Bacillus and the diagnosis of their type species were reviewed. Up to December 2008, there were 41 valid genera related with Bacillus and 112 species in the genus Bacillus,which were published in IJSEM. According to papers published in IJSEM, the characteristics, the sequence of 16S rRNA and the strain numbers of the 112 species in the genus Bacillus were redescribed and recorded.
本文对芽孢杆菌的生物学特性进行了研究,观察了枯草芽孢杆菌、简单芽孢杆菌、多粘类芽孢杆菌的细胞形态,并对几种常见芽孢杆菌的菌落形态进行了描述,也对枯草芽孢杆菌的生长特性做了相关的研究。根据鉴定结果,统计分析了我国部分省芽孢杆菌的多样性,研究结果表明每个样点分离到芽孢杆菌的数量差异很大,每个采样点的优势种群的种类和数量也有很大差异。
15个采样点土样分离到的芽孢杆菌丰富度在2-8之间。其中,青海采样点的芽孢杆菌丰富度最小,只分离到2种;新疆、内蒙古和宁夏芽孢杆菌的丰富度最大,为8。新疆采样点的丰富度和多样性指数最高,其均匀度指数较高,生态优势度指数较低。西藏的芽孢杆菌丰富度较低,多样性指数和均匀度指数最低,生态优势度指数最高。因此物种多样性不仅与物种的丰富度有关,还可能与物种间数量差异程度有一定关系。
本文还初步研究了芽孢杆菌对香蕉枯萎病病原菌和青枯雷尔氏菌的拮抗作用,筛选到22株对香蕉枯萎病病原菌有拮抗作用,15株对青枯雷尔氏菌有拮抗作用。从空间分布来看,具有拮抗作用的菌株大部分是从北方各省分离到的。
最后,本文讲述了芽孢杆菌属属水平上的分类变化现状,叙述了芽孢杆菌属相关属的分类依据及模式种特征。截止2008年12月份,IJSEM收录的芽孢杆菌属相关属有41个,芽孢杆菌属内已知种有112个。本论文还根据IJSEM上发表的文章对112个芽孢杆菌种的特征进行整理,记录了每个种的特征、16S rRNA序列号和菌株编号。
Based on the 15 soil samples collected from 15 provinces of China, 322 Bacillus strains were isolated by spread plate method. The 322 Bacillus strains were identified through morphological characteristics, physical and biological characteristics, fatty acid and ITS sequence. There were 11 species of Bacillus, 2 species of Panebacillus and 2 species of Brevibacillus had been recognized.
The biological characteristcis of Bacillus were studied in the paper. The cell morphologies of Bacillus subtilis, Bacillus simplex and Bacillus polymyxa, the colonial morphologies of Bacillus spp., and growth characteristic of Bacillus subtilis were studied. According to the preliminary result, the diversity of Bacillus collected from 15 provinces of China , showed that the quantity, varieties and as well as dominant species of Bacillus were different from each province.
The species richness of Bacillus of 15 soil samples collected from 15 provinces of China was analyzed. The results showed that the species richness of Bacillus from Qinghai, with 2 species was the lowest and the species richness of Bacillus from Xinjiang, Iner Mongolia and Ningxia, with 8 species were the highest. The diversity index and evenness index of Bacillus from Xinjiang were higher than those from Tibet, while the ecological dominant index of Bacillus from Xinjiang were lower than that from Tibet. It indicated that the species diversity was not only related to speceis richness, but also to individual quantity difference.
The antagonistic action of Bacillus to Ralstonia solanacearum and Fusarium oxysporum was preliminarily studied. The result showed that 22 strains had the antagonistic action to Fusarium oxysporum and 15 strains had the antagonistic action Fusarium oxysporum and Ralstonia solanacearum, which were mostly isolated from the soil samples collected from the northern provinces of China.
Finally, the systematic state of Bacillus, the taxonomic evidence of the related genera of Bacillus and the diagnosis of their type species were reviewed. Up to December 2008, there were 41 valid genera related with Bacillus and 112 species in the genus Bacillus,which were published in IJSEM. According to papers published in IJSEM, the characteristics, the sequence of 16S rRNA and the strain numbers of the 112 species in the genus Bacillus were redescribed and recorded.
引文
[1]刘波.芽孢杆菌文献研究. [M].广州:广东旅游出版社, 2006, 1-1679.
[2] Ash C, Farrow J A E, Wallbanks S, et al.. Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences[J]. Lett Appl Microbiol, 1991, 13:202-206.
[3] Gordon Ruth E, Haynes William C, Pang Hor-Nay. The Genus Bacillus[M]. 1973, 107-108.
[4] Krieg, N R, Williams, Wilkins, et al.. In Bergey′s Mannual of Systemactic Bacteriology[M]. Vol. 1, 1984.
[5] Satomi Masataka, La Duc Myron T, Venkateswaran Kasthuri. Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces[J]. Int J Syst Evol Microbiol, 2006, 56:1735-1740.
[6] Jeon Cheok, Lim Jee-Min, Lee Jung-Min, et al.. Reclassification of Bacillus haloalkaliphilus Fritze1996 as Alkalibacillus haloalkaliphilus gen.nov.,comb.nov.and the description of Alkalibacillus salilacus sp.nov., a novel halophilic bacterium isolated from a salt lake in China[J]. Int J Syst Evol Microbiol, 2005, 55: 1891-1896.
[7] Banat Ibrahim M, Marchant Roger, Jrahman Thahira. Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment and reassignment of Bacillus pallidus to Geobacillus pallidus comb.nov. [J]. Int J Syst Evol Microbiol, 2004, 54: 2197-2201.
[8]宋亚军,杨瑞馥,郭兆彪,等.若干需氧芽孢杆菌芽孢脂肪酸成分分析[J].微生物学报, 2001, 28(1):23-28.
[9]杨俊豪,胡学智.地衣芽孢杆菌A404耐高温α-淀粉酶的研究[J].微生物学报, 1990, 17(4): 286-289.
[10]郭成栓,崔堂兵,郭勇.嗜碱芽孢杆菌产碱性纤维素酶研究概况[J].氨基酸和生物资源, 2007, 29(1):35-38.
[11]张辉,李培军,胡筱敏,等.固定化芽孢杆菌修复油污染地表水的研究[J].中国给水排水, 2007, 23(7):8-32.
[12]金志雄,吴文琴,张珍.盾叶薯蓣淀粉对枯草芽孢杆菌α-淀粉酶活性的影响[J].微生物学杂志, 2004, 24(4):23-24.
[13]刘秀花.芽孢杆菌的应用究及进展[J].商丘师范学院学报, 2005, 21(5):135-137.
[14]林代炎,叶美锋,林琰,等. 15N示踪法研究固氮芽孢杆菌应用效果[J].生态环境, 2006, l5(6):1310-1312.
[15]胡容平,邓香洁,龚国淑,等.成都市郊区土壤芽孢杆菌的解磷、解钾潜力[J].四川农业大学学报, 2008, 26(2):167-169.
[16]宫占元,王艳杰,李永鹏,等.侧孢芽孢杆菌降解有机磷能力的研究[J].黑龙江八一农垦大学学报, 2006, 18(1):12-14.
[17]张高峡,卢振宗.作物根际联合固氮芽孢杆菌分离鉴定及生态分布[J].氨基酸和生物资源, 1998, 21(1):12-16.
[18]邢介帅,李然,赵蕾,等.产蛋白酶生防细菌的筛选及其对病原真菌的拮抗作用[J].西北农业学报, 2008, 17(1):106-109.
[19]陈莉,缪卫国,刘海洋,等.短短芽孢杆菌A57对棉花主要病原真菌的拮抗机理[J].西北农业学报, 2008, l7(4):l49-l55.
[20]方敦煌,吴祖建,邓云龙,等.防治烟草赤星病拮抗根际芽孢杆菌的筛选[J].植物病理学报, 2006, 36(6):555-561.
[21]姚丽瑾,王琦,付学池,等.小麦纹枯病生防芽孢杆菌的筛选及鉴定[J].中国生物防治, 2008, 24(1):53-57.
[22]陈弟,殷晓敏,张荣意.内生枯草芽孢杆菌B215对香蕉炭疽菌抑制作用初探[J].广西热带农业, 2008, 1:1-2.
[23]李国建,凝结芽孢杆菌替代抗生素对猪生产性能的影响[J].河南农业科学, 2004, 10:72-74.
[24]张柯,宁德冈,徐卫东.枯草芽孢杆菌芽孢表面展示重组抗原疫苗研究进展[J].微生物学报, 2006, 33(5):134-137.
[25]黄志立,罗立新,杨汝德,等.纳豆激酶[J].生命的化学, 2002, 2:82-83.
[26]张滨,马美湖.蜡状芽孢杆菌对血红蛋白降解特性研究[J].肉品卫生,2005, (7):18-22.
[27]路福平,戚薇.凝结芽孢杆菌TQ33所产抗菌物质及特性[J].中国乳品工业, 1997, 25(4):15-17.
[28]赵运胜,赵利娜,杨根庆,等.苛求芽孢杆菌胞外尿酸酶的表征及其在血清尿酸测定中的应用[J].华中科技大学学报(医学版), 2007, 36(2):239-243.
[29]蒋光吉,徐云,傅红春,等.蜡样芽孢杆菌治疗对肝硬化患者肠道菌群的影响及其意义研究[J].西南国防医药, 2008, 18(2):180-181.
[30]钟文辉,蔡祖聪.土壤微生物多样性研究方法[J].应用生态学报, 2004, 15(5):899-904.
[31] Pinto L M, Azambuja A O, Diehl E, et al.. Pathogenicity of Bacillus thuringiensis isolated from two species of Acromyrmex ( Hymenoptera , Formici2dae) [J]. Braz J Biol, 2003, 63(2):301-306.
[32] Schnepf E, Crickmoren Rie J V. Bacillus thuringiensis and its pesticidal crystal proteins[J]. Microbiol Mol Biol Rev,1998, 62:775-806.
[33] Prabagaran S R, Nimal S J, Jayachandran S. Phenotypic and genetic diversity of Bacillus thuringiensis strains isolated in India active against Spodoptera litura [J]. Appl Biochem Biotechnol, 2002, 102/ 103 (1/ 6):213-226.
[34] Uribe D, Martinezw, Ceron J. Distribution and diversity of cry genes in native strains of Bacillus thuringiensis obtained from different ecosystems from Colombia [J]. Invertebr Pathol, 2003, 82(2):119-127.
[35] Vilasboas Lemosmv. Diversity of cry genes and genetic characterization of Bacillus thuringiensis isolated fromBrazil [J]. Can J Microbiol, 2004, 50(8):605 - 613.
[36]戴莲韵,王学聘,杨光滢,等.我国森林土壤中苏云金芽孢杆菌生态分布的研究[J].微生物学报, 1994, 34(6):449-456.
[37]王学聘,戴莲韵,杨光滢,等.我国西北干旱地区森林土壤中苏云金芽孢杆菌生态分布[J].林业科学研究, 1999, 12(5):467-473.
[38]魏雪生,陈颖,牛静怡.天津地区土壤中苏云金芽孢杆菌的调查[J].天津农业学报, 2006, 13(3):30-32.
[39]苏旭东,张伟,袁耀武,等.河北省部分地区苏云金芽孢杆菌菌株的多样性[J].安徽农业科学, 2007, 35(18) :5540-5541.
[40]张华勇,李振高,王俊华,等.红壤生态系统下芽孢杆菌的物种多样性[J].土壤, 2003, 1:45-47.
[41]唐志燕,龚国淑,刘萍,等.成都市郊区土壤芽孢杆菌的初步研究[J].西南农业大学学报(自然科学版), 2005, 27(2):188-193.
[42]钱存柔,黄仪秀.微生物学实验教程[M].北京大学出版社, 1997.
[43]东秀珠,蔡妙英,等.常见细菌系统鉴定手册[M].科学出版社, 2002.
[44] Sherlock Microbial Identification System,Version 4.5, MIS Operating Mannual[M]. Newark, DE:MIDI, Inc., 2002.
[45] Xu Dong, C?te Jean-Charles. Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3′end 16S rDNA and 5′end 16S–23S ITS nucleotide sequences[J]. Int J Syst Evol Microbiol, 2003, 53:695-704.
[46]秦巧玲,粟婉媛,吕均,等. GC-MS联用分析测定志贺菌全细胞脂肪酸组成[J].中国卫生检验杂志, 2008, 18(3):426-428.
[47]刘志辉,蔡杏姗,竺澎波,等.应用气相色谱技术分析全细胞脂肪酸快速鉴定分枝杆菌[J].中国结核和呼吸杂志, 2005, 28(6):403-406.
[48]郑雪松,杨虹,李道棠,等.基因间隔序列(ITS)在细菌分类鉴定和种群分析中的应用[J].应用与环境生物学报, 2003, 9(6):678-684.
[49]辛玉华,东秀珠,吴明强. ITS序列同源性在苏云金芽孢杆菌分型中的应用研究[J].微生物学通报, 27(3):178-181.
[50]吴愉萍,徐建明,汪海珍,等. Sherlock MIS系统应用于土壤细菌鉴定的研究[J].土壤学报, 2006, 43(4):642-647.
[51]张晓舟,徐剑宏,李顺鹏.植病生防芽孢杆菌的分离筛选与初步鉴定[J].土壤, 2005, 37(1):85-88.
[52]张深,吴洪田,霍沁建,等.烟草青枯病拮抗菌的筛选及抑菌活性的测定[J].烟草科技, 2007, 6:56-58.
[53]潘惠霞,张元明,程争鸣,等.古尔班通古特沙漠生物结皮中一株嗜热细菌的特性[J].干旱区研究, 2005, 22(2):211-213.
[54] Woolard C R, Irvine R L.高盐废水在序批式反应器中的处理[J].水处理技术, 1995, 29(4):1159-1168.
[55]石春芝,陶天申,岳莹玉.神农架自然保护区九大湖芽孢杆菌资源调查[J].氨基酸和生物资源, 2001, 23 (1):1- 4.
[56] R E布钦南, N E吉本斯.伯杰细菌鉴定手册(第八版)[M].北京:科学出版社, 1984.
[57] Garrity George M, Bell Julia A, Lilburn Timothy G. Taxonomic Outline of the Prokaryotes Bergey′s Mannual of Systematic Bacteriology, second edition[M]. 2004.
[58]陶天申,杨瑞馥,东秀珠.原核生物系统学[M].化学工业出版社, 2007.
[59] Niimura Y, Koh E, Yanagida F, et al.. Amphibacillus xylanus gen. nov., sp. nov., a facultatively anaerobic sporeforming xylan-digesting bacterium which lacks cytochrome, quinone, and catalase[J]. Int J Syst Bacteriol, 1990, 40:297-301.
[60] Wisotzkey J D, Jurtshuk J P, Fox G E, et al.. Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. [J]. Int J Syst Bacteriol., 1992, 42:263-269.
[61] Golovacheva R S, Karavaiko G I. Sulfobacillus, a new genus of thermophilic sporeforming bacteria[J]. Mikrobiologiya, 1978, 47:815-822.
[62] Spring S, Ludwig W, Marquez M C, et al.. Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov., and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. [J]. Int J Syst Bacteriol, 1996, 46:492-496.
[63] Wain? M, Tindall B J, Schumann P, et al.. Gracilibacillus gen. nov., with description of Gracilibacillus halotoleransgen. nov., sp. nov., transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. [J]. Int J Syst Bacteriol, 1999, 49:821-831.
[64] Heyndrickx M, Lebbe L, Kersters K, et al.. Virgibacillus: a new genus to accommodate Bacillus pantothentics (Proom and Knight1950). Emended description of Virgibacillus pantothenticus[J]. Int J Syst Bacteriol, 1998, 48:99-106.
[65] Pikuta E, Lysenko A, Chuvilskaya N, et al.. Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavithermus comb. nov.[J]. Int J Syst Evol Microbiol, 2000, 50:2109-2117.
[66] Yoon, J H, Weiss, Lee N, Lee K C, et al.. Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with L-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen. nov., comb. nov. [J]. Int J Syst Evol Microbiol, 2001.51:2087-2093.
[67] Nazina T N, Tourova T P, Poltaraus A B, et al.. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans[J]. Int J Syst Evol Microbiol, 2001, 51:433-446.
[68] Fortina M G., Pukall R, Schumann P et al.. Ureibacillus gen. nov., a new genus to accomodate Bacillus thermosphaericus (Andersson et al. 1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov. [J]. Int J Syst Evol Microbiol, 2001, 51:447-455.
[69] Schlesner H, Lawson P A, Collins M D, et al.. Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-D-Glu-type peptidoglycan[J]. Int J Syst Evol Microbiol, 2001, 51:425-431.
[70] Yoon J H, Kang K H, Park Y H. Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea[J]. Int J Syst Evol Microbiol, 2002, 52:2043-2048.
[71] Lu J, Nogi Y, Takami H. Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge[J]. FEMS Microbiol. Lett., 2001, 205:291-297.
[72] Ishikawa M, Ishizaki S, Yamamoto Y, et al.. Paraliobacillus ryukyuensis gen. nov., sp. nov., a new Gram-positive, slightly halophilic, extremely halotolerant, facultative anaerobe isolated from a decomposing marine alga[J]. J. Gen. Appl. Microbiol., 2002, 48:269-279.
[73] Ash C, Priest F G, Collins D. Molecular identificationof rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus[J]. Antonie van Leeuwenhoek, 1993, 64:253-260.
[74] Shida O, Takagi H, Kadowaki K, et al.. Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. [J]. Int J Syst Bacteriol., 1996, 46:939-946.
[75] Touzelj P, Odonohue M, Debeire P, et al.. Thermobacillus xylanilyticus gen. nov., sp. nov., a new aerobic thermophilic xylan-degrading bacterium isolated from farm soil[J]. Int J Syst Evol Microbiol, 2000, 50:315-320.
[76]韩延平,杨瑞馥.需氧芽孢杆菌分类学研究进展[J].微生物免疫学进展, 2001, 29(4):73-78.
[77] Ghosh A, Bhardwaj M, Satyanarayana T, et al.. Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil[J]. Int J Syst Evol Microbiol, 2007, 57: 238-242.
[78] Zhang Li, Xu Zhihong,Bharat K. C. P,et al.. Bacillus decisifrondis sp. nov., isolated from soil underlying decaying leaf foliage[J]. Int J Syst Evol Microbiol, 2007, 57: 974-978.
[79] Carrasco I J, Márquez M C, Xue Y, et al.. Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China[J]. Int J Syst Evol Microbiol, 2007, 57:2084-2088.
[80] Peak K Kealy, Duncan Kathleen E, Veguilla William, et al.. Bacillus acidiceler sp. nov., isolated from a forensic specimen, containing Bacillus anthracis pX02 genes[J]. Int J Syst Evol Microbiol, 2007, 57:1909-1913.
[81] Kwon Soon-Wo, Lee Seon-Young, Kim Byung-Yong, et al.. Bacillus niabensis sp. nov., isolated from cotton-waste composts for mushroom cultivation[J]. Int J Syst Evol Microbiol, 2007, 57:1909-1913.
[82] Ten Leonid N, Baek Sang-Hun, Im Wan-Taek, et al.. Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field[J]. Int J Syst Evol Microbiol, 2007, 57:2532-2537.
[83] Borchert Martin S, Nielsen Preben, Graeber Ingeborg, et al.. Bacillus plakortidis sp. nov. and Bacillus murimartini sp. nov., novel alkalitolerant members of rRNA group 6[J]. Int J Syst Evol Microbiol, 2007, 57:2888-2893.
[84] Lim Jee-Min, Jeon Che Ok, Lee Jung Ro, et al.. Bacillus kribbensis sp. nov., isolated from a soil sample in Jeju, Korea[J]. Int J Syst Evol Microbiol, 2007, 57:2912-2916.
[85] Aino Kenichi, Hirota Kikue, Matsuno Toshihide, et al.. Bacillus polygoni sp. nov., a moderately halophilic, non-motile obligate alkaliphile isolated from indigo balls[J]. Int J Syst Evol Microbiol, 2008, 58:120-124.
[86] Albuquerque Luciana, Tiago Igor, Taborda Marco, et al.. Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond[J]. Int J Syst Evol Microbiol, 2008, 58:226-230.
[87] Kuisiene Nomeda, Raugala Juozas s, Spr?er Cathrin, et al.. Bacillus butanolivorans sp. nov., a species with industrialapplication for the remediation of n-butanol[J]. Int J Syst Evol Microbiol, 2008, 58:505-509.
[88] Borsodi Andrea K, Márialigeti Károly, SzabóGitta, et al.. Bacillus aurantiacus sp. nov., an alkaliphilic and moderatelyhalophilic bacterium isolated from Hungarian soda lakes[J]. Int J Syst Evol Microbiol, 2008, 58:845-851.
[89] Cerritos René, Vinuesa Pablo, Eguiarte Luis E, et al.. Bacillus coahuilensis sp. nov., a moderately halophilic species from a desiccation lagoon in the Cuatro Ciénegas Valley in Coahuila, Mexico[J]. Int J Syst Evol Microbiol, 2008, 58:919- 923.
[90] Sorokin Ivan D, Kravchenko Irina K, Tourova Tatjana P, et al.. Bacillus alkalidiazotrophicus sp. nov., a diazotrophic, low salt-tolerant alkaliphile isolated from Mongolian soda soil[J]. Int J Syst Evol Microbiol, 2008, 58:2459-2464.
[91] Xue Yanfen, Ventosa A, Wang Xiaowei, et al. Bacillus aidingensis sp. nov., a moderately halophilic bacterium isolated from Ai-Ding salt lake in China[J]. Int J Syst Evol Microbiol, 2008, 58:2828-2832.
[92] Zhou Yu, Wei Wei, Che Qiaolin, et al.. Bacillus pallidus sp. nov., isolated from forest soil[J]. Int J Syst Evol Microbiol, 2008, 58:2850-2854.
[93] Lee Jae-Chan, Lee Gye Suk, Park Dong-Jin,etal.. Bacillus alkalitelluris sp. nov., an alkaliphilic bacterium isolated from sandy soil[J]. Int J Syst Evol Microbiol, 2008, 58: 2629-2634.
[94] Albert Richard A, Archambault Julieta, Rosselló-Mora Ramón, et al.. Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin[J]. Int J Syst Evol Microbiol, 2005, 55:2125-2130.
[95] Pukall R, Schumann P, Clermont D, et al.. Bacillus aeolius DSM 15084T (5CIP 107628T) is a strain of Bacillus licheniformis[J]. Int J Syst Evol Microbiol, 2008, 58:1268-1270.
[96] Shivaji S, Chaturvedi P, Suresh K, et al.. Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes[J]. Int J Syst Evol Microbiol, 2006, 56:1465-1473.
[97] Nakamura L K. Bacillus alginolyticus sp. nov. and Bacillus chondroitinus sp. nov., Two Alginate-Degrading Species[J]. Int J Syst Bacteriol, Int J Syst Bacteriol, 1987, 37:284-286.
[98] Bae Seung Seob, Lee Jung-Hyun, Kim Sang-Jin. Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough[J]. Int J Syst Evol Microbiol, 2005, 55:1211-1215.
[99] Nakamura L K. Bacillus amylolyticus sp. nov., nom. rev., Bacillus lautus sp. nov., norn. rev., Bacillus pabuli sp. nov., norn. rev., and Bacillus validus sp. nov., norn. rev. [J]. Int J Syst Bacteriol, 1987, 34:224-226.
[100] Priest F G, Goodfellow M, Shute L A, et al.. Bacillus amyloliquefaciens sp. nov. norn. rev. [J]. Int J Syst Bacteriol., 1987, 37: 69-71.
[101] Shida Osamu, Takag Hiroaki, Kadowaki Kiyoshi, et al.. Bacillus aneurinolyticus sp. nov., norn. rev. [J]. Int J Syst Bacteriol, 1994, 44:143-150.
[102] Heyrman Jeroen, Rodríguez-Díaz Marina, Devos Joke, et al.. Bacillus arenosi sp. nov., Bacillus arvi sp. nov.and Bacillus humi sp. nov., isolated from soil[J]. Int J Syst Evol Microbiol, 2005, 55:111-117.
[103] Shivaji S, Suresh K, Chaturvedi Preeti, et al.. Bacillus arsenicus sp. nov., an arsenic-resistant bacterium isolated from a siderite concretion in West Bengal, India[J]. Int J Syst Evol Microbiol, 2005, 55:1123-1127.
[104] Nakamura L K. Taxonomic Relationship of Black-Pigmented Bacillus subtilis Strains and a Proposal for Bacillus atrophaeus sp. nov. [J]. Int J Syst Bacteriol., 1989, 39: 295-300.
[105] Ruiz-García Cristina, Quesada Emilia, Martínez-Checa Fernando, et al.. Bacillus axarquiensis sp. nov. and Bacillus malacitensis sp. nov., isolated from river-mouth sediments in southern Spain[J]. Int J Syst Evol Microbiol, 2005, 55:1279-1285.
[106] Pichinoty F, Debarjac H, Mandel M, et al.. Description of Bacillus azotoformans sp. nov. [J]. Int J Syst Bacteriol., 1983, 33:660-662.
[107] T?ubel Martin, K?mpfer Peter, Buczolits Sandra, et al.. Bacillus barbaricus sp. nov., isolated from an experimental wall painting[J]. Int J Syst Evol Microbiol, 2003, 53:725-730.
[108] Vargas Virginia A, Delgado Osvaldo D, Hatti-Kaul1 Rajni, et al.. Bacillus bogoriensis sp. nov., a novel alkaliphilic, halotolerant bacterium isolated from a Kenyan soda lake[J]. Int J Syst Evol Microbiol, 2005, 55:899-902.
[109] Yoon Jung-Hoon, Lee Choong-Hwan, Oh Tae-Kwang. Bacillus cibi sp. nov., isolated from jeotgal, a traditional Korean fermented seafood[J]. Int J Syst Evol Microbiol, 2005, 55:733-736.
[110] Spanka Roland, Fritze Dagmar. Bacillus cohnii sp. nov., a New, Obligately Alkaliphilic, Oval-Spore-Forming Bacillus Species with Ornithine and Aspartic Acid Instead of Diaminopimelic Acid in the Cell Wall[J]. Int. J. Syst. Bacteriol.,1993, 43:150-156.
[111] Yoshimi Kanzawa, Aktra Harada, Mariko Takeuchi, et al.. Bacillus curdlanolyticus sp. nov. and Bacillus kobensis sp. nov., Which Hydrolyze Resistant Curdlan[J]. Int J Syst Bacteriol, 1995, 45:515-521.
[112] Heyrman Jeroen, Balcaen An, Rodriguez-Diaz Marina, et al.. Bacillus decolorationis sp. nov., isolated from biodeteriorated parts of the mural paintings at the Servilia tomb (Roman necropolis of Carmona, Spain) and the Saint-Catherine chapel (Castle Herberstein, Austria) [J]. Int J Syst Evol Microbiol, 2003, 53:459-463.
[113] Kuroshima Ken Ichi, Sakane Takeshi, Tawita Renkichi, et al.. Bacillus ehimensis sp. nov. and Bacillus chitinolyticus sp. nov., New Chitinolytic Members of the Genus Bacillus[J]. Int J Syst Bacteriol, 1996, 46:76-80.
[114] Reva Oleg N, Smirnov Valerie V, Pettersson Bertil, et al. Bacillus endophyticus sp. nov., isolated from the inner tissues of cotton plants (Gossypium sp.) [J]. Int J Syst Evol Microbiol, 2002, 52:101-107.
[115] Scheldeman Patsy, Rodríguez-Díaz Marina, Goris Johan, et al.. Bacillus farraginis sp. nov., Bacillus fortis sp. nov.and Bacillus fordii sp. nov., isolated at dairy farms[J]. Int J Syst Evol Microbiol, 2004, 54:1355-1364.
[116] Tiago Igor, Pires Carlos, Mendes Vítor, et al.. Bacillus foraminis sp. nov., isolated from a non-saline alkaline groundwater[J]. Int J Syst Evol Microbiol, 2006, 56:2571-2574.
[117] Ajithkumar Vasudevan P, Ajithkumar Bindu, Iriye Ryozo, et al. Bacillus funiculus sp. nov., novel filamentous isolates from activated sludge[J]. Int J Syst Evol Microbiol, 2002, 52:1141-1144.
[118] Heyndrickx Marc, Logan Niall A, Lebbe Liesbeth, et al.. Bacillus galactosidilyticus sp. nov., an alkali-tolerantβ-galactosidase producer[J]. Int J Syst Evol Microbiol, 2004, 54:617-621.
[119] Alexande Brian r, Priest Fergus G. Bacillus glucanolyticus, a New Species That Degrades a Variety of p-Glucans[J]. Int. J. Syst. Bacteriol., 1989, 39:112-115.
[120] Wieser Monika, Worliczek Hanna, K?mpfer Peter, et al. Bacillus herbersteinensis sp. nov.[J]. Int J Syst Evol Microbiol, 2005, 55:2119-2123.
[121] Yumoto Lsao, Yamazaki Koji, Sawabe Tomoo, et al.. Bacillus horti sp. nov., a new Gram-negative alkaliphilic Bacillus[J]. Int J Syst Bacteriol, 1998, 48:565-571.
[122] Suresh K, Prabagaran S R, Sengupta S, et al.. Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India[J]. Int J Syst Evol Microbiol, 2004, 54:1369-1375.
[123] Ko Kwan Soo, Oh Won Sup, Lee Mi Young, et al.. Bacillus infantis sp. nov. and Bacillus idriensis sp.nov., isolated from a patient with neonatal sepsis[J]. Int J Syst Evol Microbiol, 2006, 56:2541-2544.
[124] Boone David R, Liu Yitai, Zhao Zhong-Ju, et al. Bacillus infernus sp. nov., an Fe (Ⅲ)-and Mn (Ⅳ)-Reducing Anaerobe from the Deep Terrestrial Subsurface[J]. Int J Syst Bacteriol,1995, 45:441-448.
[125] Yoon Jung-Hoon, Kang Seok-Sung, Lee Keun-Chul, et al.. Bacillus jeotgali sp. nov., isolated from jeotgal, Korean traditional fermented seafood[J]. Int J Syst Evol Microbiol, 2001, 51:1087-1092.
[126] Lim Jee-Min, Jeon Che Ok, Lee Jae-Chan, et al. Bacillus koreensis sp. nov., a spore-forming bacterium, isolated from the rhizosphere of willow roots in Korea[J]. Int J Syst Evol Mi crobiol, 2006, 56:59-63.
[127] Yoon Jung-Hoon, Oh Tae-Kwang. Bacillus litoralis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea[J]. Int J Syst Evol Microbiol, 2005, 55:1945-1948.
[128] Logan Niall A, Lebbe Liesbeth, Verhelst An, et al.. Bacillus luciferensis sp. nov., from volcanic soil on Candlemas Island, South Sandwich archipelago[J]. Int J Syst Evol Microbiol, 2002, 52:1985-1989.
[129] Zhang Tong, Fan Xiaojun, Hanada Satoshi, et al.. Bacillus macauensis sp. nov., a long-chain bacterium isolated from a drinking water supply[J]. Int J Syst Evol Microbiol, 2006, 56:349-353.
[130] Arahal David R, Marquez M Carmen, Volcani Benjamin E, et al. Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea[J]. Int J Syst Bacteriol, 1999, 49:521 -530.
[131] Glazunova Olga O, Raoult Didier, Roux Véronique. Bacillus massiliensis sp. nov., isolated from cerebrospinal fluid[J]. Int J Syst Evol Microbiol, 2006, 56:1485-1488.
[132] Arefman Nico, Dukhuizen Lubbert, Kirchhof Gudrun, et al.. Bacillus methanolicus sp. nov., a New Species of Thermotolerant, Methanol-Utilizing, Endospore-Forming Bacteria[J]. Int J Syst Bacteriol, 1992, 42: 439-445.
[133] Roberts Michael S, Nakamura L K, Cow Frederick M. Bacillus mojavensis sp. nov., Distinguishable from Bacillus subtilis by Sexual Isolation, Divergence in DNA Sequence, and Differences in Fatty Acid Composition[J]. Int J Syst Bacteriol, 1994, 44:256-264.
[134] Venkateswaran Kasthuri, Kempf Michael, Chen Fei, et al.. Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores areγ-radiation resistant[J]. Int J Syst Evol Microbiol, 2003, 53:165-172.
[135] Nagel M, Andreesen J R. Bacillus niacini sp. nov. a Nicotinate-Metabolizing Mesophile Isolated from soil[J]. Int J Syst Bacteriol,1991, 41, 134-139.
[136] Heyrman Jeroen, Vanparys Bram, Logan Niall A, et al.. Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands[J]. Int J Syst Evol Microbiol, 2004, 54:47-57.
[137] La Duc Myron T, Satomi Masataka, Venkateswaran Kasthuri. Bacillus odysseyi sp. nov., a round-spore-forming bacillus isolated from the Mars Odyssey spacecraft[J]. Int J Syst Evol Microbiol, 2004, 54:195-201.
[138] Nowlan Bianca, Dodia Mital S, Singh Satya P, et al.. Bacillus okhensis sp. nov., a halotolerant and alkalitolerant bacterium from an Indian saltpan[J]. Int J Syst Evol Microbiol, 2006, 56:1073-1077.
[139] Li Zhiyu, Kawamura Yoshiaki, Shida Osamu, et al.. Bacillus okuhidensis sp. nov., isolated from the Okuhida spa area of Japan[J]. Int J Syst Evol Microbiol, 2002, 52:1205-1209.
[140] Yumoto Isao, Hirota Kikue, Goto Toshitaka, et al.. Bacillus oshimensis sp. nov., a moderately halophilic, non-motile alkaliphile[J]. Int J Syst Evol Microbiol, 2005, 55:907-911.
[141] Ten Leonid N, Sang-Hoon Baek, Wan-Taek Im, et al.. Bacillus panaciterrae sp. nov., isolated from soil of a ginseng field[J]. Int J Syst Evol Microbiol, 2006, 56:2861-2866.
[142] Olivera Nelda, Si?eriz1 Faustino, Breccia Javier D. Bacillus patagoniensis sp. nov., a novel alkalitolerant bacterium from the rhizosphere of Atriplex lampa in Patagonia, Argentina[J]. Int J Syst Evol Microbiol, 2005, 55: 443-447.
[143] Lim Jee-Min, Jeon Che Ok, Lee Sang-Mi, et al.. Bacillus salarius sp. nov., a halophilic, sporeforming bacterium isolated from a salt lake in China[J]. Int J Syst Evol Microbiol, 2006, 56:373-377.
[144] Montefusco Anthony, Nakamura L K, Labeda D P. Bacillus peoriae sp. nov. [J]. Int J Syst Bacteriol, 1993, 43:388-390.
[145] Nakamura L K. Bacillus pseudomycoides sp. nov. [J]. Int J Syst Bacteriol, 1998, 48:1031-1 035.
[146] Nakamura L K. Bacillus pulvifaciens sp. nov., nom. rev. [J]. Int J Syst Bacteriol, 1984, 34:410-413.
[147] Nakamura L K, Shida O, Takagi H, et al.. Bacillus pycnus sp. nov. and Bacillus neidei sp. nov., round-spored bacteria from soil[J]. Int J Syst Bacteriol, 2002, 52:501-505.
[148] Heyndrickx Marc, Scheldeman Patsy, Forsyth Gillian, et al.. Bacillus ruris sp. nov., from dairy farms[J]. Int J Syst Evol Microbiol, 2005, 55:2551-255.
[149] Garabito Maria J, Arahal David R, Mellado Encarnacion, et al.. Bacillus salexigens sp. nov., a Halophilic Bacillus New Moderately Species[J]. Int J Syst Bacteriol, 1997, 47:735-741.
[150] Romano Ida, Lama Licia, Nicolaus Barbara, et al.. Bacillus saliphilus sp. nov., isolated from a mineral pool in Campania, Italy[J]. Int J Syst Evol Microbiol, 2005, 55:159-163.
[151] Jae-Chan Lee, Jee-Min Lim, Dong-Jin Park, et al.. Bacillus seohaeanensis sp. nov., a halotolerant bacterium that contains L-lysine in its cell wall[J]. Int J Syst Evol Microbiol, 2006, 56:1893-1898.
[152] Rheims Holger, Fruhling Anja, Schumann Peter, et al.. Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wal[J]l. Int J Syst Bacteriol, 1999, 49:795-802.
[153] Pettersson Bertil, Silva SK de, Mathias Uhlen, et al.. Bacillus siralis sp. nov., a novel species from silage with a higher order structural attribute in the 16S rRNA genes[J]. Int J Syst Evol Microbiol, 2000, 50:2181-2187.
[154] Palmisano Margaret M, Nakamura L K, Duncan Kathleen E, et al.. Bacillus sonorensis sp. nov., a close relative of Bacillus licheniformis, isolated from soil in the Sonoran Desert, Arizona[J]. Int J Syst Evol Microbiol, 2001, 51:1671-1679.
[155] Pettersson Bertil, Lembke Fritz, Hammer Philipp, et al.. Bacillus sporothemodurans, a New Species Producing Heat-Resistant Endospores Highly[J]. Int J Syst Bacteriol, 1996, 46:759-764.
[156] Lawrence K. Nakamura, Inge Blumenstock, Dieter Claus. Taxonomic Study of Bacillus coagulans Hammer 1915 with a Proposal for Bacillus srnithii sp. nov. [J]. Int J Syst Bacteriol, 1988, 38:63-73.
[157] Kanso Sungwan, Greene Anthony C, Bharat KCP, et al.. Bacillus subterraneus sp. nov., an iron-and manganese-reducing bacterium from a deep subsurface Australian thermal aquifer[J]. Int J Syst Evol Microbiol, 2002, 52: 869-874.
[158] Jee-Min Lim, Che Ok Jeon, Chang-Jin Kim. Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea[J]. Int J Syst Evol Microbiol, 2006, 56:2903-2908.
[159] Joshua W Gatson, Bruce F Benz, Chitra Chandrasekaran, et al.. Bacillus tequilensis sp. nov., isolated from a 2000-year-old Mexican shaft-tomb, is closely related to Bacillus subtilis[J]. Int J Syst Evol Microbiol, 2006, 56:1475-1484.
[160] Combet-Blanc Y, Ollivier B, Streicher C, et al.. Bacillus themoamylovorans sp. nov., a Moderately Thermophilic and Amylolytic Bacterium[J]. Int J Syst Bacteriol, 1995, 45:9-16.
[161] Manachini P L, Fortina M G, Parini C, Craveri R. Bacillus thermoruber sp. nov. nom. rev., a Red-PigmentedThermophilic Bacterium[J]. Int J Syst Bacteriol, 1985, 35:493-496.
[162] Nakamura L K. Bacillus thiaminolyticus sp. nov., norn. rev. [J]. Int J Syst Bacteriol, 1990, 40:242-246.
[163] Michael S Roberts, L K Nakamura, Frederick M. Cohan. Bacillus vallismortis sp. nov., a Close Relative of Bacillus subtilis, isolated from Soil in Death Valley, California[J]. Int J Syst Bacteriol, 1996, 46: 470-475.
[164] Ruiz-García Cristina, Béjar Victoria, Martínez-Checa Fernando, et al.. Bacillus velezensis sp. nov., a surfactant- producing bacterium isolated from the river Vélez in Málaga, southern Spain. [J] Int J Syst Evol Microbiol, 2005, 55:191-195.
[165] Caccamo D, Gugliandolo C, Stackebrandt E, et al.. Bacillus vulcani sp. nov., a novel thermophilic species isolated from a shallow marine hydrothermalvent[J]. Int J Syst Evol Microbiol, 2000, 50:2009-2012.
[2] Ash C, Farrow J A E, Wallbanks S, et al.. Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences[J]. Lett Appl Microbiol, 1991, 13:202-206.
[3] Gordon Ruth E, Haynes William C, Pang Hor-Nay. The Genus Bacillus[M]. 1973, 107-108.
[4] Krieg, N R, Williams, Wilkins, et al.. In Bergey′s Mannual of Systemactic Bacteriology[M]. Vol. 1, 1984.
[5] Satomi Masataka, La Duc Myron T, Venkateswaran Kasthuri. Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces[J]. Int J Syst Evol Microbiol, 2006, 56:1735-1740.
[6] Jeon Cheok, Lim Jee-Min, Lee Jung-Min, et al.. Reclassification of Bacillus haloalkaliphilus Fritze1996 as Alkalibacillus haloalkaliphilus gen.nov.,comb.nov.and the description of Alkalibacillus salilacus sp.nov., a novel halophilic bacterium isolated from a salt lake in China[J]. Int J Syst Evol Microbiol, 2005, 55: 1891-1896.
[7] Banat Ibrahim M, Marchant Roger, Jrahman Thahira. Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment and reassignment of Bacillus pallidus to Geobacillus pallidus comb.nov. [J]. Int J Syst Evol Microbiol, 2004, 54: 2197-2201.
[8]宋亚军,杨瑞馥,郭兆彪,等.若干需氧芽孢杆菌芽孢脂肪酸成分分析[J].微生物学报, 2001, 28(1):23-28.
[9]杨俊豪,胡学智.地衣芽孢杆菌A404耐高温α-淀粉酶的研究[J].微生物学报, 1990, 17(4): 286-289.
[10]郭成栓,崔堂兵,郭勇.嗜碱芽孢杆菌产碱性纤维素酶研究概况[J].氨基酸和生物资源, 2007, 29(1):35-38.
[11]张辉,李培军,胡筱敏,等.固定化芽孢杆菌修复油污染地表水的研究[J].中国给水排水, 2007, 23(7):8-32.
[12]金志雄,吴文琴,张珍.盾叶薯蓣淀粉对枯草芽孢杆菌α-淀粉酶活性的影响[J].微生物学杂志, 2004, 24(4):23-24.
[13]刘秀花.芽孢杆菌的应用究及进展[J].商丘师范学院学报, 2005, 21(5):135-137.
[14]林代炎,叶美锋,林琰,等. 15N示踪法研究固氮芽孢杆菌应用效果[J].生态环境, 2006, l5(6):1310-1312.
[15]胡容平,邓香洁,龚国淑,等.成都市郊区土壤芽孢杆菌的解磷、解钾潜力[J].四川农业大学学报, 2008, 26(2):167-169.
[16]宫占元,王艳杰,李永鹏,等.侧孢芽孢杆菌降解有机磷能力的研究[J].黑龙江八一农垦大学学报, 2006, 18(1):12-14.
[17]张高峡,卢振宗.作物根际联合固氮芽孢杆菌分离鉴定及生态分布[J].氨基酸和生物资源, 1998, 21(1):12-16.
[18]邢介帅,李然,赵蕾,等.产蛋白酶生防细菌的筛选及其对病原真菌的拮抗作用[J].西北农业学报, 2008, 17(1):106-109.
[19]陈莉,缪卫国,刘海洋,等.短短芽孢杆菌A57对棉花主要病原真菌的拮抗机理[J].西北农业学报, 2008, l7(4):l49-l55.
[20]方敦煌,吴祖建,邓云龙,等.防治烟草赤星病拮抗根际芽孢杆菌的筛选[J].植物病理学报, 2006, 36(6):555-561.
[21]姚丽瑾,王琦,付学池,等.小麦纹枯病生防芽孢杆菌的筛选及鉴定[J].中国生物防治, 2008, 24(1):53-57.
[22]陈弟,殷晓敏,张荣意.内生枯草芽孢杆菌B215对香蕉炭疽菌抑制作用初探[J].广西热带农业, 2008, 1:1-2.
[23]李国建,凝结芽孢杆菌替代抗生素对猪生产性能的影响[J].河南农业科学, 2004, 10:72-74.
[24]张柯,宁德冈,徐卫东.枯草芽孢杆菌芽孢表面展示重组抗原疫苗研究进展[J].微生物学报, 2006, 33(5):134-137.
[25]黄志立,罗立新,杨汝德,等.纳豆激酶[J].生命的化学, 2002, 2:82-83.
[26]张滨,马美湖.蜡状芽孢杆菌对血红蛋白降解特性研究[J].肉品卫生,2005, (7):18-22.
[27]路福平,戚薇.凝结芽孢杆菌TQ33所产抗菌物质及特性[J].中国乳品工业, 1997, 25(4):15-17.
[28]赵运胜,赵利娜,杨根庆,等.苛求芽孢杆菌胞外尿酸酶的表征及其在血清尿酸测定中的应用[J].华中科技大学学报(医学版), 2007, 36(2):239-243.
[29]蒋光吉,徐云,傅红春,等.蜡样芽孢杆菌治疗对肝硬化患者肠道菌群的影响及其意义研究[J].西南国防医药, 2008, 18(2):180-181.
[30]钟文辉,蔡祖聪.土壤微生物多样性研究方法[J].应用生态学报, 2004, 15(5):899-904.
[31] Pinto L M, Azambuja A O, Diehl E, et al.. Pathogenicity of Bacillus thuringiensis isolated from two species of Acromyrmex ( Hymenoptera , Formici2dae) [J]. Braz J Biol, 2003, 63(2):301-306.
[32] Schnepf E, Crickmoren Rie J V. Bacillus thuringiensis and its pesticidal crystal proteins[J]. Microbiol Mol Biol Rev,1998, 62:775-806.
[33] Prabagaran S R, Nimal S J, Jayachandran S. Phenotypic and genetic diversity of Bacillus thuringiensis strains isolated in India active against Spodoptera litura [J]. Appl Biochem Biotechnol, 2002, 102/ 103 (1/ 6):213-226.
[34] Uribe D, Martinezw, Ceron J. Distribution and diversity of cry genes in native strains of Bacillus thuringiensis obtained from different ecosystems from Colombia [J]. Invertebr Pathol, 2003, 82(2):119-127.
[35] Vilasboas Lemosmv. Diversity of cry genes and genetic characterization of Bacillus thuringiensis isolated fromBrazil [J]. Can J Microbiol, 2004, 50(8):605 - 613.
[36]戴莲韵,王学聘,杨光滢,等.我国森林土壤中苏云金芽孢杆菌生态分布的研究[J].微生物学报, 1994, 34(6):449-456.
[37]王学聘,戴莲韵,杨光滢,等.我国西北干旱地区森林土壤中苏云金芽孢杆菌生态分布[J].林业科学研究, 1999, 12(5):467-473.
[38]魏雪生,陈颖,牛静怡.天津地区土壤中苏云金芽孢杆菌的调查[J].天津农业学报, 2006, 13(3):30-32.
[39]苏旭东,张伟,袁耀武,等.河北省部分地区苏云金芽孢杆菌菌株的多样性[J].安徽农业科学, 2007, 35(18) :5540-5541.
[40]张华勇,李振高,王俊华,等.红壤生态系统下芽孢杆菌的物种多样性[J].土壤, 2003, 1:45-47.
[41]唐志燕,龚国淑,刘萍,等.成都市郊区土壤芽孢杆菌的初步研究[J].西南农业大学学报(自然科学版), 2005, 27(2):188-193.
[42]钱存柔,黄仪秀.微生物学实验教程[M].北京大学出版社, 1997.
[43]东秀珠,蔡妙英,等.常见细菌系统鉴定手册[M].科学出版社, 2002.
[44] Sherlock Microbial Identification System,Version 4.5, MIS Operating Mannual[M]. Newark, DE:MIDI, Inc., 2002.
[45] Xu Dong, C?te Jean-Charles. Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3′end 16S rDNA and 5′end 16S–23S ITS nucleotide sequences[J]. Int J Syst Evol Microbiol, 2003, 53:695-704.
[46]秦巧玲,粟婉媛,吕均,等. GC-MS联用分析测定志贺菌全细胞脂肪酸组成[J].中国卫生检验杂志, 2008, 18(3):426-428.
[47]刘志辉,蔡杏姗,竺澎波,等.应用气相色谱技术分析全细胞脂肪酸快速鉴定分枝杆菌[J].中国结核和呼吸杂志, 2005, 28(6):403-406.
[48]郑雪松,杨虹,李道棠,等.基因间隔序列(ITS)在细菌分类鉴定和种群分析中的应用[J].应用与环境生物学报, 2003, 9(6):678-684.
[49]辛玉华,东秀珠,吴明强. ITS序列同源性在苏云金芽孢杆菌分型中的应用研究[J].微生物学通报, 27(3):178-181.
[50]吴愉萍,徐建明,汪海珍,等. Sherlock MIS系统应用于土壤细菌鉴定的研究[J].土壤学报, 2006, 43(4):642-647.
[51]张晓舟,徐剑宏,李顺鹏.植病生防芽孢杆菌的分离筛选与初步鉴定[J].土壤, 2005, 37(1):85-88.
[52]张深,吴洪田,霍沁建,等.烟草青枯病拮抗菌的筛选及抑菌活性的测定[J].烟草科技, 2007, 6:56-58.
[53]潘惠霞,张元明,程争鸣,等.古尔班通古特沙漠生物结皮中一株嗜热细菌的特性[J].干旱区研究, 2005, 22(2):211-213.
[54] Woolard C R, Irvine R L.高盐废水在序批式反应器中的处理[J].水处理技术, 1995, 29(4):1159-1168.
[55]石春芝,陶天申,岳莹玉.神农架自然保护区九大湖芽孢杆菌资源调查[J].氨基酸和生物资源, 2001, 23 (1):1- 4.
[56] R E布钦南, N E吉本斯.伯杰细菌鉴定手册(第八版)[M].北京:科学出版社, 1984.
[57] Garrity George M, Bell Julia A, Lilburn Timothy G. Taxonomic Outline of the Prokaryotes Bergey′s Mannual of Systematic Bacteriology, second edition[M]. 2004.
[58]陶天申,杨瑞馥,东秀珠.原核生物系统学[M].化学工业出版社, 2007.
[59] Niimura Y, Koh E, Yanagida F, et al.. Amphibacillus xylanus gen. nov., sp. nov., a facultatively anaerobic sporeforming xylan-digesting bacterium which lacks cytochrome, quinone, and catalase[J]. Int J Syst Bacteriol, 1990, 40:297-301.
[60] Wisotzkey J D, Jurtshuk J P, Fox G E, et al.. Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. [J]. Int J Syst Bacteriol., 1992, 42:263-269.
[61] Golovacheva R S, Karavaiko G I. Sulfobacillus, a new genus of thermophilic sporeforming bacteria[J]. Mikrobiologiya, 1978, 47:815-822.
[62] Spring S, Ludwig W, Marquez M C, et al.. Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov., and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. [J]. Int J Syst Bacteriol, 1996, 46:492-496.
[63] Wain? M, Tindall B J, Schumann P, et al.. Gracilibacillus gen. nov., with description of Gracilibacillus halotoleransgen. nov., sp. nov., transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. [J]. Int J Syst Bacteriol, 1999, 49:821-831.
[64] Heyndrickx M, Lebbe L, Kersters K, et al.. Virgibacillus: a new genus to accommodate Bacillus pantothentics (Proom and Knight1950). Emended description of Virgibacillus pantothenticus[J]. Int J Syst Bacteriol, 1998, 48:99-106.
[65] Pikuta E, Lysenko A, Chuvilskaya N, et al.. Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavithermus comb. nov.[J]. Int J Syst Evol Microbiol, 2000, 50:2109-2117.
[66] Yoon, J H, Weiss, Lee N, Lee K C, et al.. Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with L-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen. nov., comb. nov. [J]. Int J Syst Evol Microbiol, 2001.51:2087-2093.
[67] Nazina T N, Tourova T P, Poltaraus A B, et al.. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans[J]. Int J Syst Evol Microbiol, 2001, 51:433-446.
[68] Fortina M G., Pukall R, Schumann P et al.. Ureibacillus gen. nov., a new genus to accomodate Bacillus thermosphaericus (Andersson et al. 1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov. [J]. Int J Syst Evol Microbiol, 2001, 51:447-455.
[69] Schlesner H, Lawson P A, Collins M D, et al.. Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-D-Glu-type peptidoglycan[J]. Int J Syst Evol Microbiol, 2001, 51:425-431.
[70] Yoon J H, Kang K H, Park Y H. Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea[J]. Int J Syst Evol Microbiol, 2002, 52:2043-2048.
[71] Lu J, Nogi Y, Takami H. Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge[J]. FEMS Microbiol. Lett., 2001, 205:291-297.
[72] Ishikawa M, Ishizaki S, Yamamoto Y, et al.. Paraliobacillus ryukyuensis gen. nov., sp. nov., a new Gram-positive, slightly halophilic, extremely halotolerant, facultative anaerobe isolated from a decomposing marine alga[J]. J. Gen. Appl. Microbiol., 2002, 48:269-279.
[73] Ash C, Priest F G, Collins D. Molecular identificationof rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus[J]. Antonie van Leeuwenhoek, 1993, 64:253-260.
[74] Shida O, Takagi H, Kadowaki K, et al.. Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. [J]. Int J Syst Bacteriol., 1996, 46:939-946.
[75] Touzelj P, Odonohue M, Debeire P, et al.. Thermobacillus xylanilyticus gen. nov., sp. nov., a new aerobic thermophilic xylan-degrading bacterium isolated from farm soil[J]. Int J Syst Evol Microbiol, 2000, 50:315-320.
[76]韩延平,杨瑞馥.需氧芽孢杆菌分类学研究进展[J].微生物免疫学进展, 2001, 29(4):73-78.
[77] Ghosh A, Bhardwaj M, Satyanarayana T, et al.. Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil[J]. Int J Syst Evol Microbiol, 2007, 57: 238-242.
[78] Zhang Li, Xu Zhihong,Bharat K. C. P,et al.. Bacillus decisifrondis sp. nov., isolated from soil underlying decaying leaf foliage[J]. Int J Syst Evol Microbiol, 2007, 57: 974-978.
[79] Carrasco I J, Márquez M C, Xue Y, et al.. Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China[J]. Int J Syst Evol Microbiol, 2007, 57:2084-2088.
[80] Peak K Kealy, Duncan Kathleen E, Veguilla William, et al.. Bacillus acidiceler sp. nov., isolated from a forensic specimen, containing Bacillus anthracis pX02 genes[J]. Int J Syst Evol Microbiol, 2007, 57:1909-1913.
[81] Kwon Soon-Wo, Lee Seon-Young, Kim Byung-Yong, et al.. Bacillus niabensis sp. nov., isolated from cotton-waste composts for mushroom cultivation[J]. Int J Syst Evol Microbiol, 2007, 57:1909-1913.
[82] Ten Leonid N, Baek Sang-Hun, Im Wan-Taek, et al.. Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field[J]. Int J Syst Evol Microbiol, 2007, 57:2532-2537.
[83] Borchert Martin S, Nielsen Preben, Graeber Ingeborg, et al.. Bacillus plakortidis sp. nov. and Bacillus murimartini sp. nov., novel alkalitolerant members of rRNA group 6[J]. Int J Syst Evol Microbiol, 2007, 57:2888-2893.
[84] Lim Jee-Min, Jeon Che Ok, Lee Jung Ro, et al.. Bacillus kribbensis sp. nov., isolated from a soil sample in Jeju, Korea[J]. Int J Syst Evol Microbiol, 2007, 57:2912-2916.
[85] Aino Kenichi, Hirota Kikue, Matsuno Toshihide, et al.. Bacillus polygoni sp. nov., a moderately halophilic, non-motile obligate alkaliphile isolated from indigo balls[J]. Int J Syst Evol Microbiol, 2008, 58:120-124.
[86] Albuquerque Luciana, Tiago Igor, Taborda Marco, et al.. Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond[J]. Int J Syst Evol Microbiol, 2008, 58:226-230.
[87] Kuisiene Nomeda, Raugala Juozas s, Spr?er Cathrin, et al.. Bacillus butanolivorans sp. nov., a species with industrialapplication for the remediation of n-butanol[J]. Int J Syst Evol Microbiol, 2008, 58:505-509.
[88] Borsodi Andrea K, Márialigeti Károly, SzabóGitta, et al.. Bacillus aurantiacus sp. nov., an alkaliphilic and moderatelyhalophilic bacterium isolated from Hungarian soda lakes[J]. Int J Syst Evol Microbiol, 2008, 58:845-851.
[89] Cerritos René, Vinuesa Pablo, Eguiarte Luis E, et al.. Bacillus coahuilensis sp. nov., a moderately halophilic species from a desiccation lagoon in the Cuatro Ciénegas Valley in Coahuila, Mexico[J]. Int J Syst Evol Microbiol, 2008, 58:919- 923.
[90] Sorokin Ivan D, Kravchenko Irina K, Tourova Tatjana P, et al.. Bacillus alkalidiazotrophicus sp. nov., a diazotrophic, low salt-tolerant alkaliphile isolated from Mongolian soda soil[J]. Int J Syst Evol Microbiol, 2008, 58:2459-2464.
[91] Xue Yanfen, Ventosa A, Wang Xiaowei, et al. Bacillus aidingensis sp. nov., a moderately halophilic bacterium isolated from Ai-Ding salt lake in China[J]. Int J Syst Evol Microbiol, 2008, 58:2828-2832.
[92] Zhou Yu, Wei Wei, Che Qiaolin, et al.. Bacillus pallidus sp. nov., isolated from forest soil[J]. Int J Syst Evol Microbiol, 2008, 58:2850-2854.
[93] Lee Jae-Chan, Lee Gye Suk, Park Dong-Jin,etal.. Bacillus alkalitelluris sp. nov., an alkaliphilic bacterium isolated from sandy soil[J]. Int J Syst Evol Microbiol, 2008, 58: 2629-2634.
[94] Albert Richard A, Archambault Julieta, Rosselló-Mora Ramón, et al.. Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin[J]. Int J Syst Evol Microbiol, 2005, 55:2125-2130.
[95] Pukall R, Schumann P, Clermont D, et al.. Bacillus aeolius DSM 15084T (5CIP 107628T) is a strain of Bacillus licheniformis[J]. Int J Syst Evol Microbiol, 2008, 58:1268-1270.
[96] Shivaji S, Chaturvedi P, Suresh K, et al.. Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes[J]. Int J Syst Evol Microbiol, 2006, 56:1465-1473.
[97] Nakamura L K. Bacillus alginolyticus sp. nov. and Bacillus chondroitinus sp. nov., Two Alginate-Degrading Species[J]. Int J Syst Bacteriol, Int J Syst Bacteriol, 1987, 37:284-286.
[98] Bae Seung Seob, Lee Jung-Hyun, Kim Sang-Jin. Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough[J]. Int J Syst Evol Microbiol, 2005, 55:1211-1215.
[99] Nakamura L K. Bacillus amylolyticus sp. nov., nom. rev., Bacillus lautus sp. nov., norn. rev., Bacillus pabuli sp. nov., norn. rev., and Bacillus validus sp. nov., norn. rev. [J]. Int J Syst Bacteriol, 1987, 34:224-226.
[100] Priest F G, Goodfellow M, Shute L A, et al.. Bacillus amyloliquefaciens sp. nov. norn. rev. [J]. Int J Syst Bacteriol., 1987, 37: 69-71.
[101] Shida Osamu, Takag Hiroaki, Kadowaki Kiyoshi, et al.. Bacillus aneurinolyticus sp. nov., norn. rev. [J]. Int J Syst Bacteriol, 1994, 44:143-150.
[102] Heyrman Jeroen, Rodríguez-Díaz Marina, Devos Joke, et al.. Bacillus arenosi sp. nov., Bacillus arvi sp. nov.and Bacillus humi sp. nov., isolated from soil[J]. Int J Syst Evol Microbiol, 2005, 55:111-117.
[103] Shivaji S, Suresh K, Chaturvedi Preeti, et al.. Bacillus arsenicus sp. nov., an arsenic-resistant bacterium isolated from a siderite concretion in West Bengal, India[J]. Int J Syst Evol Microbiol, 2005, 55:1123-1127.
[104] Nakamura L K. Taxonomic Relationship of Black-Pigmented Bacillus subtilis Strains and a Proposal for Bacillus atrophaeus sp. nov. [J]. Int J Syst Bacteriol., 1989, 39: 295-300.
[105] Ruiz-García Cristina, Quesada Emilia, Martínez-Checa Fernando, et al.. Bacillus axarquiensis sp. nov. and Bacillus malacitensis sp. nov., isolated from river-mouth sediments in southern Spain[J]. Int J Syst Evol Microbiol, 2005, 55:1279-1285.
[106] Pichinoty F, Debarjac H, Mandel M, et al.. Description of Bacillus azotoformans sp. nov. [J]. Int J Syst Bacteriol., 1983, 33:660-662.
[107] T?ubel Martin, K?mpfer Peter, Buczolits Sandra, et al.. Bacillus barbaricus sp. nov., isolated from an experimental wall painting[J]. Int J Syst Evol Microbiol, 2003, 53:725-730.
[108] Vargas Virginia A, Delgado Osvaldo D, Hatti-Kaul1 Rajni, et al.. Bacillus bogoriensis sp. nov., a novel alkaliphilic, halotolerant bacterium isolated from a Kenyan soda lake[J]. Int J Syst Evol Microbiol, 2005, 55:899-902.
[109] Yoon Jung-Hoon, Lee Choong-Hwan, Oh Tae-Kwang. Bacillus cibi sp. nov., isolated from jeotgal, a traditional Korean fermented seafood[J]. Int J Syst Evol Microbiol, 2005, 55:733-736.
[110] Spanka Roland, Fritze Dagmar. Bacillus cohnii sp. nov., a New, Obligately Alkaliphilic, Oval-Spore-Forming Bacillus Species with Ornithine and Aspartic Acid Instead of Diaminopimelic Acid in the Cell Wall[J]. Int. J. Syst. Bacteriol.,1993, 43:150-156.
[111] Yoshimi Kanzawa, Aktra Harada, Mariko Takeuchi, et al.. Bacillus curdlanolyticus sp. nov. and Bacillus kobensis sp. nov., Which Hydrolyze Resistant Curdlan[J]. Int J Syst Bacteriol, 1995, 45:515-521.
[112] Heyrman Jeroen, Balcaen An, Rodriguez-Diaz Marina, et al.. Bacillus decolorationis sp. nov., isolated from biodeteriorated parts of the mural paintings at the Servilia tomb (Roman necropolis of Carmona, Spain) and the Saint-Catherine chapel (Castle Herberstein, Austria) [J]. Int J Syst Evol Microbiol, 2003, 53:459-463.
[113] Kuroshima Ken Ichi, Sakane Takeshi, Tawita Renkichi, et al.. Bacillus ehimensis sp. nov. and Bacillus chitinolyticus sp. nov., New Chitinolytic Members of the Genus Bacillus[J]. Int J Syst Bacteriol, 1996, 46:76-80.
[114] Reva Oleg N, Smirnov Valerie V, Pettersson Bertil, et al. Bacillus endophyticus sp. nov., isolated from the inner tissues of cotton plants (Gossypium sp.) [J]. Int J Syst Evol Microbiol, 2002, 52:101-107.
[115] Scheldeman Patsy, Rodríguez-Díaz Marina, Goris Johan, et al.. Bacillus farraginis sp. nov., Bacillus fortis sp. nov.and Bacillus fordii sp. nov., isolated at dairy farms[J]. Int J Syst Evol Microbiol, 2004, 54:1355-1364.
[116] Tiago Igor, Pires Carlos, Mendes Vítor, et al.. Bacillus foraminis sp. nov., isolated from a non-saline alkaline groundwater[J]. Int J Syst Evol Microbiol, 2006, 56:2571-2574.
[117] Ajithkumar Vasudevan P, Ajithkumar Bindu, Iriye Ryozo, et al. Bacillus funiculus sp. nov., novel filamentous isolates from activated sludge[J]. Int J Syst Evol Microbiol, 2002, 52:1141-1144.
[118] Heyndrickx Marc, Logan Niall A, Lebbe Liesbeth, et al.. Bacillus galactosidilyticus sp. nov., an alkali-tolerantβ-galactosidase producer[J]. Int J Syst Evol Microbiol, 2004, 54:617-621.
[119] Alexande Brian r, Priest Fergus G. Bacillus glucanolyticus, a New Species That Degrades a Variety of p-Glucans[J]. Int. J. Syst. Bacteriol., 1989, 39:112-115.
[120] Wieser Monika, Worliczek Hanna, K?mpfer Peter, et al. Bacillus herbersteinensis sp. nov.[J]. Int J Syst Evol Microbiol, 2005, 55:2119-2123.
[121] Yumoto Lsao, Yamazaki Koji, Sawabe Tomoo, et al.. Bacillus horti sp. nov., a new Gram-negative alkaliphilic Bacillus[J]. Int J Syst Bacteriol, 1998, 48:565-571.
[122] Suresh K, Prabagaran S R, Sengupta S, et al.. Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India[J]. Int J Syst Evol Microbiol, 2004, 54:1369-1375.
[123] Ko Kwan Soo, Oh Won Sup, Lee Mi Young, et al.. Bacillus infantis sp. nov. and Bacillus idriensis sp.nov., isolated from a patient with neonatal sepsis[J]. Int J Syst Evol Microbiol, 2006, 56:2541-2544.
[124] Boone David R, Liu Yitai, Zhao Zhong-Ju, et al. Bacillus infernus sp. nov., an Fe (Ⅲ)-and Mn (Ⅳ)-Reducing Anaerobe from the Deep Terrestrial Subsurface[J]. Int J Syst Bacteriol,1995, 45:441-448.
[125] Yoon Jung-Hoon, Kang Seok-Sung, Lee Keun-Chul, et al.. Bacillus jeotgali sp. nov., isolated from jeotgal, Korean traditional fermented seafood[J]. Int J Syst Evol Microbiol, 2001, 51:1087-1092.
[126] Lim Jee-Min, Jeon Che Ok, Lee Jae-Chan, et al. Bacillus koreensis sp. nov., a spore-forming bacterium, isolated from the rhizosphere of willow roots in Korea[J]. Int J Syst Evol Mi crobiol, 2006, 56:59-63.
[127] Yoon Jung-Hoon, Oh Tae-Kwang. Bacillus litoralis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea[J]. Int J Syst Evol Microbiol, 2005, 55:1945-1948.
[128] Logan Niall A, Lebbe Liesbeth, Verhelst An, et al.. Bacillus luciferensis sp. nov., from volcanic soil on Candlemas Island, South Sandwich archipelago[J]. Int J Syst Evol Microbiol, 2002, 52:1985-1989.
[129] Zhang Tong, Fan Xiaojun, Hanada Satoshi, et al.. Bacillus macauensis sp. nov., a long-chain bacterium isolated from a drinking water supply[J]. Int J Syst Evol Microbiol, 2006, 56:349-353.
[130] Arahal David R, Marquez M Carmen, Volcani Benjamin E, et al. Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea[J]. Int J Syst Bacteriol, 1999, 49:521 -530.
[131] Glazunova Olga O, Raoult Didier, Roux Véronique. Bacillus massiliensis sp. nov., isolated from cerebrospinal fluid[J]. Int J Syst Evol Microbiol, 2006, 56:1485-1488.
[132] Arefman Nico, Dukhuizen Lubbert, Kirchhof Gudrun, et al.. Bacillus methanolicus sp. nov., a New Species of Thermotolerant, Methanol-Utilizing, Endospore-Forming Bacteria[J]. Int J Syst Bacteriol, 1992, 42: 439-445.
[133] Roberts Michael S, Nakamura L K, Cow Frederick M. Bacillus mojavensis sp. nov., Distinguishable from Bacillus subtilis by Sexual Isolation, Divergence in DNA Sequence, and Differences in Fatty Acid Composition[J]. Int J Syst Bacteriol, 1994, 44:256-264.
[134] Venkateswaran Kasthuri, Kempf Michael, Chen Fei, et al.. Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores areγ-radiation resistant[J]. Int J Syst Evol Microbiol, 2003, 53:165-172.
[135] Nagel M, Andreesen J R. Bacillus niacini sp. nov. a Nicotinate-Metabolizing Mesophile Isolated from soil[J]. Int J Syst Bacteriol,1991, 41, 134-139.
[136] Heyrman Jeroen, Vanparys Bram, Logan Niall A, et al.. Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands[J]. Int J Syst Evol Microbiol, 2004, 54:47-57.
[137] La Duc Myron T, Satomi Masataka, Venkateswaran Kasthuri. Bacillus odysseyi sp. nov., a round-spore-forming bacillus isolated from the Mars Odyssey spacecraft[J]. Int J Syst Evol Microbiol, 2004, 54:195-201.
[138] Nowlan Bianca, Dodia Mital S, Singh Satya P, et al.. Bacillus okhensis sp. nov., a halotolerant and alkalitolerant bacterium from an Indian saltpan[J]. Int J Syst Evol Microbiol, 2006, 56:1073-1077.
[139] Li Zhiyu, Kawamura Yoshiaki, Shida Osamu, et al.. Bacillus okuhidensis sp. nov., isolated from the Okuhida spa area of Japan[J]. Int J Syst Evol Microbiol, 2002, 52:1205-1209.
[140] Yumoto Isao, Hirota Kikue, Goto Toshitaka, et al.. Bacillus oshimensis sp. nov., a moderately halophilic, non-motile alkaliphile[J]. Int J Syst Evol Microbiol, 2005, 55:907-911.
[141] Ten Leonid N, Sang-Hoon Baek, Wan-Taek Im, et al.. Bacillus panaciterrae sp. nov., isolated from soil of a ginseng field[J]. Int J Syst Evol Microbiol, 2006, 56:2861-2866.
[142] Olivera Nelda, Si?eriz1 Faustino, Breccia Javier D. Bacillus patagoniensis sp. nov., a novel alkalitolerant bacterium from the rhizosphere of Atriplex lampa in Patagonia, Argentina[J]. Int J Syst Evol Microbiol, 2005, 55: 443-447.
[143] Lim Jee-Min, Jeon Che Ok, Lee Sang-Mi, et al.. Bacillus salarius sp. nov., a halophilic, sporeforming bacterium isolated from a salt lake in China[J]. Int J Syst Evol Microbiol, 2006, 56:373-377.
[144] Montefusco Anthony, Nakamura L K, Labeda D P. Bacillus peoriae sp. nov. [J]. Int J Syst Bacteriol, 1993, 43:388-390.
[145] Nakamura L K. Bacillus pseudomycoides sp. nov. [J]. Int J Syst Bacteriol, 1998, 48:1031-1 035.
[146] Nakamura L K. Bacillus pulvifaciens sp. nov., nom. rev. [J]. Int J Syst Bacteriol, 1984, 34:410-413.
[147] Nakamura L K, Shida O, Takagi H, et al.. Bacillus pycnus sp. nov. and Bacillus neidei sp. nov., round-spored bacteria from soil[J]. Int J Syst Bacteriol, 2002, 52:501-505.
[148] Heyndrickx Marc, Scheldeman Patsy, Forsyth Gillian, et al.. Bacillus ruris sp. nov., from dairy farms[J]. Int J Syst Evol Microbiol, 2005, 55:2551-255.
[149] Garabito Maria J, Arahal David R, Mellado Encarnacion, et al.. Bacillus salexigens sp. nov., a Halophilic Bacillus New Moderately Species[J]. Int J Syst Bacteriol, 1997, 47:735-741.
[150] Romano Ida, Lama Licia, Nicolaus Barbara, et al.. Bacillus saliphilus sp. nov., isolated from a mineral pool in Campania, Italy[J]. Int J Syst Evol Microbiol, 2005, 55:159-163.
[151] Jae-Chan Lee, Jee-Min Lim, Dong-Jin Park, et al.. Bacillus seohaeanensis sp. nov., a halotolerant bacterium that contains L-lysine in its cell wall[J]. Int J Syst Evol Microbiol, 2006, 56:1893-1898.
[152] Rheims Holger, Fruhling Anja, Schumann Peter, et al.. Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wal[J]l. Int J Syst Bacteriol, 1999, 49:795-802.
[153] Pettersson Bertil, Silva SK de, Mathias Uhlen, et al.. Bacillus siralis sp. nov., a novel species from silage with a higher order structural attribute in the 16S rRNA genes[J]. Int J Syst Evol Microbiol, 2000, 50:2181-2187.
[154] Palmisano Margaret M, Nakamura L K, Duncan Kathleen E, et al.. Bacillus sonorensis sp. nov., a close relative of Bacillus licheniformis, isolated from soil in the Sonoran Desert, Arizona[J]. Int J Syst Evol Microbiol, 2001, 51:1671-1679.
[155] Pettersson Bertil, Lembke Fritz, Hammer Philipp, et al.. Bacillus sporothemodurans, a New Species Producing Heat-Resistant Endospores Highly[J]. Int J Syst Bacteriol, 1996, 46:759-764.
[156] Lawrence K. Nakamura, Inge Blumenstock, Dieter Claus. Taxonomic Study of Bacillus coagulans Hammer 1915 with a Proposal for Bacillus srnithii sp. nov. [J]. Int J Syst Bacteriol, 1988, 38:63-73.
[157] Kanso Sungwan, Greene Anthony C, Bharat KCP, et al.. Bacillus subterraneus sp. nov., an iron-and manganese-reducing bacterium from a deep subsurface Australian thermal aquifer[J]. Int J Syst Evol Microbiol, 2002, 52: 869-874.
[158] Jee-Min Lim, Che Ok Jeon, Chang-Jin Kim. Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea[J]. Int J Syst Evol Microbiol, 2006, 56:2903-2908.
[159] Joshua W Gatson, Bruce F Benz, Chitra Chandrasekaran, et al.. Bacillus tequilensis sp. nov., isolated from a 2000-year-old Mexican shaft-tomb, is closely related to Bacillus subtilis[J]. Int J Syst Evol Microbiol, 2006, 56:1475-1484.
[160] Combet-Blanc Y, Ollivier B, Streicher C, et al.. Bacillus themoamylovorans sp. nov., a Moderately Thermophilic and Amylolytic Bacterium[J]. Int J Syst Bacteriol, 1995, 45:9-16.
[161] Manachini P L, Fortina M G, Parini C, Craveri R. Bacillus thermoruber sp. nov. nom. rev., a Red-PigmentedThermophilic Bacterium[J]. Int J Syst Bacteriol, 1985, 35:493-496.
[162] Nakamura L K. Bacillus thiaminolyticus sp. nov., norn. rev. [J]. Int J Syst Bacteriol, 1990, 40:242-246.
[163] Michael S Roberts, L K Nakamura, Frederick M. Cohan. Bacillus vallismortis sp. nov., a Close Relative of Bacillus subtilis, isolated from Soil in Death Valley, California[J]. Int J Syst Bacteriol, 1996, 46: 470-475.
[164] Ruiz-García Cristina, Béjar Victoria, Martínez-Checa Fernando, et al.. Bacillus velezensis sp. nov., a surfactant- producing bacterium isolated from the river Vélez in Málaga, southern Spain. [J] Int J Syst Evol Microbiol, 2005, 55:191-195.
[165] Caccamo D, Gugliandolo C, Stackebrandt E, et al.. Bacillus vulcani sp. nov., a novel thermophilic species isolated from a shallow marine hydrothermalvent[J]. Int J Syst Evol Microbiol, 2000, 50:2009-2012.