扑热息痛分子印迹膜电化学传感器的研究
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摘要
扑热息痛(Paracetamol,PR)是世界上使用最广泛的非处方药品之一,具有解热镇痛的作用,过量使用该药则会引起不良反应和严重肝损害等后果。本文将分子印迹技术与电化学检测技术相结合,成功制备了三种扑热息痛分子印迹电化学传感器。本论文主要工作如下:
①分子印迹膜电化学聚合法的优点在于传质较快、膜厚度易控制及成膜均匀等。本实验以扑热息痛为模板分子,邻苯二胺为功能单体,采用电聚合法制备了一种新型的扑热息痛分子印迹电化学传感器。实验中以铁氰化钾为分子探针,采用方波伏安法对扑热息痛进行了检测,结果表明该传感器对扑热息痛具有良好的选择性和灵敏度。扑热息痛的浓度与K_3[Fe(CN)_6]的相对峰电流在2.0×10~(-6)～8.0×10~(-5)mol/L范围内成线性关系,检出限为2.0×10~(-8)mol/L。将此传感器用于药物中扑热息痛含量的检测,可取得良好的结果。
②以扑热息痛为模板分子,等摩尔的间苯二酚与邻苯二胺为功能单体,采用循环伏安法合成了稳定的扑热息痛分子印迹膜。在含5mmol/L K_3Fe(CN)_6和0.5 mol/L KCl的支持电解质溶液中,用循环伏安法和交流阻抗法对此双功能单体所制备的分子印迹膜进行了表征,结果表明该印迹膜具有一定的绝缘性。研究了不同印迹电极对同一浓度扑热息痛溶液的响应性能,结果发现采用双功能单体所制备的印迹电极比采用单一功能单体时制备的电极响应信号更强。采用方波伏安法直接检测扑热息痛在此传感器上的电流响应,扑热息痛的氧化峰电流与其浓度在5.0×10~(-7)～3.0×10~(-50mol/L范围内成线性关系,检出限为2.0×10~(-8)mol/L。
③基于多壁碳纳米管(MWNTs)的高电催化活性及分子印迹聚合物(MIPs)的特异识别性,本实验以扑热息痛分子为模板,邻苯二胺为单体,在多壁碳纳米管修饰玻碳电极(MWNTs/GCE)上合成了扑热息痛分子印迹膜,以此构建了一种基于分子印迹-多壁碳纳米管的扑热息痛电化学传感器。通过比较复合膜电极(MIP/MWNTs/GCE)与印迹膜电极(MIP/GCE)对相同浓度扑热息痛溶液的电流响应,发现MWNTs的加入确实有效地提高了传感器的灵敏度。扑热息痛的浓度与其在该传感器上的方波伏安响应在2.0×10~(-6)～8.0×10~(-5)mol/L范围内具有良好线性关系(R~2=0.9993),检测限可达1.0×10~(-8) mol/L。该传感器的响应时间为5min,具有良好的稳定性及重现性。
Paracetamol(PR) is one of the world’s most widely used nonprescription medicines, due to its analgesic’s and antipyretic’s properties. However, overdoses of PR cause adverse reaction and liver damage. In this study, three kinds of PR electrochemical sensors were constructed based on molecular imprinting technology and electrochemistry analysis technology. The main work of this thesis is as follows:
①A new molecularly imprinted polymer(MIP) sensor for PR was fabricated by electropolymerization in this work, with PR as template molecule and o-phenylenediamine(o-PD) as monomer. The K_3Fe(CN)_6 was used as a molecular probe, and the sensor exhibited good selectivity and sensitivity to PR. The square wave voltammetry response of potassium ferricyanide was linear to the concentration of PR in the range of 2.0×10~(-6)~8.0×10~(-5)mol/L, with a detection limit of 2.0×10~(-8)mol/L. The proposed sensor was applied to determination of PR in different tablets and the obtained results were satisfactory.
②Molecularly imprinted polymer modified glassy carbon electrode(MIP/GCE) was prepared by electropolymerization of equimolar resorcinol and o-PD on glassy carbon electrode in the presence of PR as the template. The property of MIP/GCE is investigated by cyclic voltammetry and AC impedance. And the response properties of different electrode in the same concentration of PR is investigated by cyclic voltammetry, it was found that the response current of the MIP/GCE prepared by dual-monomer is larger than which prepared by a single monomer. Square wave voltammetry was used to the direct detection of PR on the MIP/GCE.
③A novel MIP sensor was constructed for detection of PR. The sensitive layer was synthesized on the multiwall carbon nanotubes modified glassy carbon electrode (MWNTs/GCE) with PR molecule as a template and o-PD as monomer. The results showed that MIP/MWNTs/GCE had an obvious electrocatalytic effect on PR. The square wave voltammetry response of PR was linear to its concentration in the range from 2.0×10~(-6) to 8.0×10~(-5)mol/L (R2=0.9993), with a detection limit of 1.0×10~(-8)mol/L. The sensor exhibited a good stability and reproducibility.
①分子印迹膜电化学聚合法的优点在于传质较快、膜厚度易控制及成膜均匀等。本实验以扑热息痛为模板分子,邻苯二胺为功能单体,采用电聚合法制备了一种新型的扑热息痛分子印迹电化学传感器。实验中以铁氰化钾为分子探针,采用方波伏安法对扑热息痛进行了检测,结果表明该传感器对扑热息痛具有良好的选择性和灵敏度。扑热息痛的浓度与K_3[Fe(CN)_6]的相对峰电流在2.0×10~(-6)～8.0×10~(-5)mol/L范围内成线性关系,检出限为2.0×10~(-8)mol/L。将此传感器用于药物中扑热息痛含量的检测,可取得良好的结果。
②以扑热息痛为模板分子,等摩尔的间苯二酚与邻苯二胺为功能单体,采用循环伏安法合成了稳定的扑热息痛分子印迹膜。在含5mmol/L K_3Fe(CN)_6和0.5 mol/L KCl的支持电解质溶液中,用循环伏安法和交流阻抗法对此双功能单体所制备的分子印迹膜进行了表征,结果表明该印迹膜具有一定的绝缘性。研究了不同印迹电极对同一浓度扑热息痛溶液的响应性能,结果发现采用双功能单体所制备的印迹电极比采用单一功能单体时制备的电极响应信号更强。采用方波伏安法直接检测扑热息痛在此传感器上的电流响应,扑热息痛的氧化峰电流与其浓度在5.0×10~(-7)～3.0×10~(-50mol/L范围内成线性关系,检出限为2.0×10~(-8)mol/L。
③基于多壁碳纳米管(MWNTs)的高电催化活性及分子印迹聚合物(MIPs)的特异识别性,本实验以扑热息痛分子为模板,邻苯二胺为单体,在多壁碳纳米管修饰玻碳电极(MWNTs/GCE)上合成了扑热息痛分子印迹膜,以此构建了一种基于分子印迹-多壁碳纳米管的扑热息痛电化学传感器。通过比较复合膜电极(MIP/MWNTs/GCE)与印迹膜电极(MIP/GCE)对相同浓度扑热息痛溶液的电流响应,发现MWNTs的加入确实有效地提高了传感器的灵敏度。扑热息痛的浓度与其在该传感器上的方波伏安响应在2.0×10~(-6)～8.0×10~(-5)mol/L范围内具有良好线性关系(R~2=0.9993),检测限可达1.0×10~(-8) mol/L。该传感器的响应时间为5min,具有良好的稳定性及重现性。
Paracetamol(PR) is one of the world’s most widely used nonprescription medicines, due to its analgesic’s and antipyretic’s properties. However, overdoses of PR cause adverse reaction and liver damage. In this study, three kinds of PR electrochemical sensors were constructed based on molecular imprinting technology and electrochemistry analysis technology. The main work of this thesis is as follows:
①A new molecularly imprinted polymer(MIP) sensor for PR was fabricated by electropolymerization in this work, with PR as template molecule and o-phenylenediamine(o-PD) as monomer. The K_3Fe(CN)_6 was used as a molecular probe, and the sensor exhibited good selectivity and sensitivity to PR. The square wave voltammetry response of potassium ferricyanide was linear to the concentration of PR in the range of 2.0×10~(-6)~8.0×10~(-5)mol/L, with a detection limit of 2.0×10~(-8)mol/L. The proposed sensor was applied to determination of PR in different tablets and the obtained results were satisfactory.
②Molecularly imprinted polymer modified glassy carbon electrode(MIP/GCE) was prepared by electropolymerization of equimolar resorcinol and o-PD on glassy carbon electrode in the presence of PR as the template. The property of MIP/GCE is investigated by cyclic voltammetry and AC impedance. And the response properties of different electrode in the same concentration of PR is investigated by cyclic voltammetry, it was found that the response current of the MIP/GCE prepared by dual-monomer is larger than which prepared by a single monomer. Square wave voltammetry was used to the direct detection of PR on the MIP/GCE.
③A novel MIP sensor was constructed for detection of PR. The sensitive layer was synthesized on the multiwall carbon nanotubes modified glassy carbon electrode (MWNTs/GCE) with PR molecule as a template and o-PD as monomer. The results showed that MIP/MWNTs/GCE had an obvious electrocatalytic effect on PR. The square wave voltammetry response of PR was linear to its concentration in the range from 2.0×10~(-6) to 8.0×10~(-5)mol/L (R2=0.9993), with a detection limit of 1.0×10~(-8)mol/L. The sensor exhibited a good stability and reproducibility.
引文
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