Azu-1及PinX1蛋白质在细胞有丝分裂中的功能解析
摘要
在动物细胞中,中心体是主要的微管组织中心,它在细胞极性的产生,胞间运输以及细胞分裂过程中发挥着重要作用。细胞通过调控中心体的正常复制与分离,可以防止细胞分裂时染色体的异常分离。当中心体功能出现障碍时,往往伴随染色体分离异常,从而导致癌症的发生。
作为Aurora serine/threonine蛋白激酶家族的成员之一,Aurora A定位于中心体,在细胞有丝分裂过程中,调节中心体的成熟和双极纺锤体的组装。Aurora A的缺失会导致中心粒的分离出现问题,或者令染色体不能正确的排列到赤道板上。最近的研究的发现,在果蝇和爪蟾系统中,Aurora A能够磷酸化TACC(Transforming acidic coiled coil)蛋白,并招募TACC蛋白到中心体上,与微管结合蛋白一起参与调控中心体微管的稳定性。人类存在三种TACC蛋白(TACC1、TACC2和TACC3),其中TACC3已经被证明能够被Aurora A磷酸化,而有关于TACC2的报道却很少。在这里,我们主要研究了人类TACC家族中的Azu-1(anti-zuai-1),它是TACC2蛋白的异构体。我们的研究结果表明Azu-1通过其N端与Aurora A相互作用,Azu-1与Aurora A在有丝分裂期的HeLa细胞中共定位于中心体,并且Azu-1能够被Aurora A磷酸化,这个磷酸酸化事件对于Azu-1定位到中心体上是相当重要的。
另一方面,在细胞分裂过程中,从DNA的复制到染色体的配对,以及与微管相互作用,到最后正确分离到子代细胞,这一系列重要事件都受到细胞周期检验点的精密监视及调控。而当染色体出现错误时或者动点没有完全被微管捕获时,纺锤体装配检验点(Spindle assembly checkpoint)被激活。随后一系列信号转导事件将修正染色体动点与微管之间不正确的连接,最后动点完全正确的排列在赤道板上,细胞周期得以从中期进入后期。纺锤体装配检验点可以保证染色体正确分离。从最简单的单细胞真核生物酵母,到复杂的后生生物,例如我们人类自己,这些进行调控的分子事件都是高度保守的。
PinX1是一个潜在的间期端粒酶抑制因子,它在细胞有丝分裂期中的报道很少。在这里我们证明了PinX1在染色体的正常分离过程中起着很重要的作用。去卷积显微镜观察结果显示PinX1在间期时定位于核仁和端粒,在有丝分裂期时定位于染色体周边以及动点上。我们设计的缺失突变体实验证明了PinX1的动点定位依赖于它的中间区域,而染色体周边的定位依赖于它的C端。有趣的是,PinX1的动点定位依赖于Hec1和CENP-E,同时我们的生化数据显示PinX1是一个重要的微管结合蛋白。我们的实时图像分析表明在抑制了PinX1的表达后,染色体不能正常的排列到赤道板上,导致有丝分裂期染色体桥以及间期小核的发生,意味着PinX1在染色体稳定性方面所起的作用。另外,我们还找到了PinX1的结合蛋白Nucleolin,它是一个染色体周边蛋白,通过PinX1的C端与PinX1相互作用。去卷积显微镜结果显示在前中期时,PinX1主要与Nucleolin共定位于染色体周边。更进一步的研究发现,Nucleolin的缺失会破坏PinX1的染色体周边定位,暗示着PinX1与Nucleolin在功能上的相互关系。实时图像数据表明,当PinX1和Nucleolin的表达同时被抑制时,染色体的分离不能正常进行。我们认为PinX1能够被Nucleolin招募到染色体周边,而PinX1和Nucleolin复合物对于染色体正常排列到赤道板这个过程中起着重要作用。
In animal cells, centrosome, also called microtubule organization center (MTOC), plays an important part in plasticity of cell polarity, assists with introcellular transporting and participates in the management of mitotic behaviors. Accurate replication and segregation of centrosomes are the premises of daughter cell division, avoiding false separation of chromosomes. Thus, centrosome abnormalities can lead to chromosome instability and cancer.
As a member of Aurora serine/threonine kinase family, Aurora A plays a significant role in maturation of centrosomes and assembly of bipolar spindles related to its centrosome localization during mitosis. Suppression of Aurora A causes errors when centriole pairs separate, or chromosomes congress to the spindle equator. Recently, Xenopus and Drosophila transforming acidic coiled coil (TACC) proteins have been found to be the substrates of Aurora A. After phosphorylation by Aurora A, TACC proteins are recruited to centrosomes, and then contribute to modulating centrosomal microtubule stability with microtubule associated proteins. Three TACC proteins (TACC1, TACC2, TACC3) exist in human, and TACC3 has been proved to be phosphorylated by Aurora A. However little is known about TACC2. In this study, we focus on anti-zuai-1 (Azu-1), which is identified as an isoform of TACC2. We have demonstrated that Azu-1 interacts with Aurora A through its N-terminal region and co-localizes with Aurora A at centrosomes in mitotic HeLa cells. Moreover, Azu-1 can be phosphorylated by Aurora A kinase and the phosphorylation is essential for centrosomal localization of Azu-1.
On the other hand, most of the important things, comprising DNA’s replication, interaction of chromosomes with microtubules and accurate segregation to the daughter cells, are monitored by the cell cycle checkpoint in the procession of cell division. When error occurs, spindle assembly checkpoint will be activated, which assures that kinetochore could align precisely on the equatorial plate and cell cycle continues from metaphase to anaphase. Spindle assembly checkpoint warrants the exact separation of chromosomes. Mechanisms concerning these cell cycle events are conserved from the simple mono-cell eukaryote to the complicated metazoan.
PinX1 is a potent telomerase inhibitor in interphase; however, its function in mitosis is not well documented. Here we show that PinX1 is essential for faithful chromosome segregation. Deconvolution microscopic analyses show that PinX1 localizes to nucleoli and telomeres in interphase and relocates to the periphery of chromosomes and outer plate of the kinetochores in mitosis. Our deletion analyses mapped PinX1’s kinetochore localization domain to the central region and its chromosome periphery localization domain to the C terminus. Interestingly, the kinetochore localization of PinX1 is dependent on Hec1 and CENP-E. Our biochemical characterization revealed that PinX1 is a novel microtubule-binding protein. Our real-time imaging analyses show that suppression of PinX1 by small interference RNA abrogates faithful chromosome segregation and results in anaphase chromatid bridges in mitosis and micronuclei in interphase, suggesting an essential role of PinX1 in chromosome stability. Furthermore we report that PinX1 interacts with Nucleolin, a chromosome periphery protein, through its C-termini. Deconvolution microscopic analyses show PinX1 mainly co-localizes with Nucleolin at chromosome periphery in prometaphase. Moreover, depletion of Nucleolin abolishes chromosome periphery localizations of PinX1, suggesting a functional interrelationship between PinX1 and Nucleolin. Importantly, repression of PinX1 and Nucleolin abrogates chromosome segregation in real-time mitosis, validating the functional importance of PinX1-Nucleolin interaction. We propose PinX1 is recruited to chromosome periphery by Nucleolin and a complex of PinX1 and Nucleolin is essential for faithful chromosome congression.
作为Aurora serine/threonine蛋白激酶家族的成员之一,Aurora A定位于中心体,在细胞有丝分裂过程中,调节中心体的成熟和双极纺锤体的组装。Aurora A的缺失会导致中心粒的分离出现问题,或者令染色体不能正确的排列到赤道板上。最近的研究的发现,在果蝇和爪蟾系统中,Aurora A能够磷酸化TACC(Transforming acidic coiled coil)蛋白,并招募TACC蛋白到中心体上,与微管结合蛋白一起参与调控中心体微管的稳定性。人类存在三种TACC蛋白(TACC1、TACC2和TACC3),其中TACC3已经被证明能够被Aurora A磷酸化,而有关于TACC2的报道却很少。在这里,我们主要研究了人类TACC家族中的Azu-1(anti-zuai-1),它是TACC2蛋白的异构体。我们的研究结果表明Azu-1通过其N端与Aurora A相互作用,Azu-1与Aurora A在有丝分裂期的HeLa细胞中共定位于中心体,并且Azu-1能够被Aurora A磷酸化,这个磷酸酸化事件对于Azu-1定位到中心体上是相当重要的。
另一方面,在细胞分裂过程中,从DNA的复制到染色体的配对,以及与微管相互作用,到最后正确分离到子代细胞,这一系列重要事件都受到细胞周期检验点的精密监视及调控。而当染色体出现错误时或者动点没有完全被微管捕获时,纺锤体装配检验点(Spindle assembly checkpoint)被激活。随后一系列信号转导事件将修正染色体动点与微管之间不正确的连接,最后动点完全正确的排列在赤道板上,细胞周期得以从中期进入后期。纺锤体装配检验点可以保证染色体正确分离。从最简单的单细胞真核生物酵母,到复杂的后生生物,例如我们人类自己,这些进行调控的分子事件都是高度保守的。
PinX1是一个潜在的间期端粒酶抑制因子,它在细胞有丝分裂期中的报道很少。在这里我们证明了PinX1在染色体的正常分离过程中起着很重要的作用。去卷积显微镜观察结果显示PinX1在间期时定位于核仁和端粒,在有丝分裂期时定位于染色体周边以及动点上。我们设计的缺失突变体实验证明了PinX1的动点定位依赖于它的中间区域,而染色体周边的定位依赖于它的C端。有趣的是,PinX1的动点定位依赖于Hec1和CENP-E,同时我们的生化数据显示PinX1是一个重要的微管结合蛋白。我们的实时图像分析表明在抑制了PinX1的表达后,染色体不能正常的排列到赤道板上,导致有丝分裂期染色体桥以及间期小核的发生,意味着PinX1在染色体稳定性方面所起的作用。另外,我们还找到了PinX1的结合蛋白Nucleolin,它是一个染色体周边蛋白,通过PinX1的C端与PinX1相互作用。去卷积显微镜结果显示在前中期时,PinX1主要与Nucleolin共定位于染色体周边。更进一步的研究发现,Nucleolin的缺失会破坏PinX1的染色体周边定位,暗示着PinX1与Nucleolin在功能上的相互关系。实时图像数据表明,当PinX1和Nucleolin的表达同时被抑制时,染色体的分离不能正常进行。我们认为PinX1能够被Nucleolin招募到染色体周边,而PinX1和Nucleolin复合物对于染色体正常排列到赤道板这个过程中起着重要作用。
In animal cells, centrosome, also called microtubule organization center (MTOC), plays an important part in plasticity of cell polarity, assists with introcellular transporting and participates in the management of mitotic behaviors. Accurate replication and segregation of centrosomes are the premises of daughter cell division, avoiding false separation of chromosomes. Thus, centrosome abnormalities can lead to chromosome instability and cancer.
As a member of Aurora serine/threonine kinase family, Aurora A plays a significant role in maturation of centrosomes and assembly of bipolar spindles related to its centrosome localization during mitosis. Suppression of Aurora A causes errors when centriole pairs separate, or chromosomes congress to the spindle equator. Recently, Xenopus and Drosophila transforming acidic coiled coil (TACC) proteins have been found to be the substrates of Aurora A. After phosphorylation by Aurora A, TACC proteins are recruited to centrosomes, and then contribute to modulating centrosomal microtubule stability with microtubule associated proteins. Three TACC proteins (TACC1, TACC2, TACC3) exist in human, and TACC3 has been proved to be phosphorylated by Aurora A. However little is known about TACC2. In this study, we focus on anti-zuai-1 (Azu-1), which is identified as an isoform of TACC2. We have demonstrated that Azu-1 interacts with Aurora A through its N-terminal region and co-localizes with Aurora A at centrosomes in mitotic HeLa cells. Moreover, Azu-1 can be phosphorylated by Aurora A kinase and the phosphorylation is essential for centrosomal localization of Azu-1.
On the other hand, most of the important things, comprising DNA’s replication, interaction of chromosomes with microtubules and accurate segregation to the daughter cells, are monitored by the cell cycle checkpoint in the procession of cell division. When error occurs, spindle assembly checkpoint will be activated, which assures that kinetochore could align precisely on the equatorial plate and cell cycle continues from metaphase to anaphase. Spindle assembly checkpoint warrants the exact separation of chromosomes. Mechanisms concerning these cell cycle events are conserved from the simple mono-cell eukaryote to the complicated metazoan.
PinX1 is a potent telomerase inhibitor in interphase; however, its function in mitosis is not well documented. Here we show that PinX1 is essential for faithful chromosome segregation. Deconvolution microscopic analyses show that PinX1 localizes to nucleoli and telomeres in interphase and relocates to the periphery of chromosomes and outer plate of the kinetochores in mitosis. Our deletion analyses mapped PinX1’s kinetochore localization domain to the central region and its chromosome periphery localization domain to the C terminus. Interestingly, the kinetochore localization of PinX1 is dependent on Hec1 and CENP-E. Our biochemical characterization revealed that PinX1 is a novel microtubule-binding protein. Our real-time imaging analyses show that suppression of PinX1 by small interference RNA abrogates faithful chromosome segregation and results in anaphase chromatid bridges in mitosis and micronuclei in interphase, suggesting an essential role of PinX1 in chromosome stability. Furthermore we report that PinX1 interacts with Nucleolin, a chromosome periphery protein, through its C-termini. Deconvolution microscopic analyses show PinX1 mainly co-localizes with Nucleolin at chromosome periphery in prometaphase. Moreover, depletion of Nucleolin abolishes chromosome periphery localizations of PinX1, suggesting a functional interrelationship between PinX1 and Nucleolin. Importantly, repression of PinX1 and Nucleolin abrogates chromosome segregation in real-time mitosis, validating the functional importance of PinX1-Nucleolin interaction. We propose PinX1 is recruited to chromosome periphery by Nucleolin and a complex of PinX1 and Nucleolin is essential for faithful chromosome congression.
引文
Anand S, Penrhyn-Lowe S, Venkitaraman AR (2003) AURORA-A amplification overrides the mitotic spindle assembly checkpoint, inducing resistance to Taxol. Cancer Cell 3(1): 51-62
Andreassen PR, Lohez OD, Lacroix FB, Margolis RL (2001) Tetraploid state induces p53-dependent arrest of nontransformed mammalian cells in G1. Mol Biol Cell 12(5): 1315-1328
Andrews PD, Knatko E, Moore WJ, Swedlow JR (2003) Mitotic mechanics: the auroras come into view. Curr Opin Cell Biol 15(6): 672-683
Andrews PD, Ovechkina Y, Morrice N, Wagenbach M, Duncan K, Wordeman L, Swedlow JR (2004) Aurora B regulates MCAK at the mitotic centromere. Dev Cell 6(2): 253-268
Artandi SE, DePinho RA (2000) A critical role for telomeres in suppressing and facilitating carcinogenesis. Curr Opin Genet Dev 10(1): 39-46
Barros TP, Kinoshita K, Hyman AA, Raff JW (2005) Aurora A activates D-TACC-Msps complexes exclusively at centrosomes to stabilize centrosomal microtubules. The Journal of cell biology 170(7): 1039-1046
Bellanger JM, Gonczy P (2003) TAC-1 and ZYG-9 form a complex that promotes microtubule assembly in C. elegans embryos. Curr Biol 13(17): 1488-1498
Berdnik D, Knoblich JA (2002) Drosophila Aurora-A is required for centrosome maturation and actin-dependent asymmetric protein localization during mitosis. Curr Biol 12(8): 640-647
Bianchi A, Smith S, Chong L, Elias P, de Lange T (1997) TRF1 is a dimer and bends telomeric DNA. The EMBO journal 16(7): 1785-1794
Bischoff JR, Anderson L, Zhu Y, Mossie K, Ng L, Souza B, Schryver B, Flanagan P, Clairvoyant F, Ginther C, Chan CS, Novotny M, Slamon DJ, Plowman GD (1998) A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers. The EMBO journal 17(11): 3052-3065
Blasco MA, Lee HW, Hande MP, Samper E, Lansdorp PM, DePinho RA, Greider CW (1997) Telomere shortening and tumor formation by mouse cells lacking telomerase RNA. Cell 91(1): 25-34
Bobinnec Y, Khodjakov A, Mir LM, Rieder CL, Edde B, Bornens M (1998) Centriole disassembly in vivo and its effect on centrosome structure and function in vertebrate cells. The Journal of cell biology 143(6): 1575-1589
Bodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu CP, Morin GB, Harley CB, Shay JW, Lichtsteiner S, Wright WE (1998) Extension of life-span by introduction of telomerase intonormal human cells. Science (New York, NY 279(5349): 349-352
Cheeseman IM, Anderson S, Jwa M, Green EM, Kang J, Yates JR, 3rd, Chan CS, Drubin DG, Barnes G (2002) Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p. Cell 111(2): 163-172
Chen Y, Yang Y, van Overbeek M, Donigian JR, Baciu P, de Lange T, Lei M (2008) A Shared Docking Motif in TRF1 and TRF2 Used for Differential Recruitment of Telomeric Proteins. Science (New York, NY
Chen Z, Indjeian VB, McManus M, Wang L, Dynlacht BD (2002) CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells. Dev Cell 3(3): 339-350
Conrad MN, Wright JH, Wolf AJ, Zakian VA (1990) RAP1 protein interacts with yeast telomeres in vivo: overproduction alters telomere structure and decreases chromosome stability. Cell 63(4): 739-750
Conte N, Delaval B, Ginestier C, Ferrand A, Isnardon D, Larroque C, Prigent C, Seraphin B, Jacquemier J, Birnbaum D (2003) TACC1-chTOG-Aurora A protein complex in breast cancer. Oncogene 22(50): 8102-8116
Cooper JP, Nimmo ER, Allshire RC, Cech TR (1997) Regulation of telomere length and function by a Myb-domain protein in fission yeast. Nature 385(6618): 744-747
Cullen CF, Ohkura H (2001) Msps protein is localized to acentrosomal poles to ensure bipolarity of Drosophila meiotic spindles. Nat Cell Biol 3(7): 637-642
de Saint Phalle B, Sullivan W (1998) Spindle assembly and mitosis without centrosomes in parthenogenetic Sciara embryos. The Journal of cell biology 141(6): 1383-1391
Desai A, Verma S, Mitchison TJ, Walczak CE (1999) Kin I kinesins are microtubule-destabilizing enzymes. Cell 96(1): 69-78
Diviani D, Scott JD (2001) AKAP signaling complexes at the cytoskeleton. J Cell Sci 114(Pt 8): 1431-1437
Doxsey S, Zimmerman W, Mikule K (2005) Centrosome control of the cell cycle. Trends Cell Biol 15(6): 303-311
Du J, Hannon GJ (2004) Suppression of p160ROCK bypasses cell cycle arrest after Aurora-A/STK15 depletion. Proceedings of the National Academy of Sciences of the United States of America 101(24): 8975-8980
Dutertre S, Descamps S, Prigent C (2002) On the role of aurora-A in centrosome function. Oncogene 21(40): 6175-6183
Dutertre S, Hamard-Peron E, Cremet JY, Thomas Y, Prigent C (2005) The absence of p53 aggravates polyploidy and centrosome number abnormality induced by Aurora-Coverexpression. Cell cycle (Georgetown, Tex 4(12): 1783-1787
Elliott S, Knop M, Schlenstedt G, Schiebel E (1999) Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication. Proceedings of the National Academy of Sciences of the United States of America 96(11): 6205-6210
Eto M, Elliott E, Prickett TD, Brautigan DL (2002) Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation. J Biol Chem 277(46): 44013-44020
Evans SK, Lundblad V (1999) Est1 and Cdc13 as comediators of telomerase access. Science (New York, NY 286(5437): 117-120
Eyers PA, Erikson E, Chen LG, Maller JL (2003) A novel mechanism for activation of the protein kinase Aurora A. Curr Biol 13(8): 691-697
Fairall L, Chapman L, Moss H, de Lange T, Rhodes D (2001) Structure of the TRFH dimerization domain of the human telomeric proteins TRF1 and TRF2. Mol Cell 8(2): 351-361
Feng J, Funk WD, Wang SS, Weinrich SL, Avilion AA, Chiu CP, Adams RR, Chang E, Allsopp RC, Yu J, et al. (1995) The RNA component of human telomerase. Science (New York, NY 269(5228): 1236-1241
Fry AM, Mayor T, Meraldi P, Stierhof YD, Tanaka K, Nigg EA (1998a) C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2. The Journal of cell biology 141(7): 1563-1574
Fry AM, Meraldi P, Nigg EA (1998b) A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators. The EMBO journal 17(2): 470-481
Fujiwara T, Bandi M, Nitta M, Ivanova EV, Bronson RT, Pellman D (2005) Cytokinesis failure generating tetraploids promotes tumorigenesis in p53-null cells. Nature 437(7061): 1043-1047
Gadde S, Heald R (2004) Mechanisms and molecules of the mitotic spindle. Curr Biol 14(18): R797-805
Gadea BB, Ruderman JV (2006) Aurora B is required for mitotic chromatin-induced phosphorylation of Op18/Stathmin. Proceedings of the National Academy of Sciences of the United States of America 103(12): 4493-4498
Gard DL, Becker BE, Josh Romney S (2004) MAPping the eukaryotic tree of life: structure, function, and evolution of the MAP215/Dis1 family of microtubule-associated proteins. Int Rev Cytol 239: 179-272
Gard DL, Kirschner MW (1987) A microtubule-associated protein from Xenopus eggs that specifically promotes assembly at the plus-end. The Journal of cell biology 105(5): 2203-2215
Gergely F (2002) Centrosomal TACCtics. Bioessays 24(10): 915-925
Gergely F, Draviam VM, Raff JW (2003) The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells. Genes Dev 17(3): 336-341
Gergely F, Karlsson C, Still I, Cowell J, Kilmartin J, Raff JW (2000) The TACC domain identifies a family of centrosomal proteins that can interact with microtubules. Proceedings of the National Academy of Sciences of the United States of America 97(26): 14352-14357
Gergely F, Kidd D, Jeffers K, Wakefield JG, Raff JW (2000) D-TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo. The EMBO journal 19(2): 241-252
Giansanti MG, Gatti M, Bonaccorsi S (2001) The role of centrosomes and astral microtubules during asymmetric division of Drosophila neuroblasts. Development 128(7): 1137-1145
Giet R, McLean D, Descamps S, Lee MJ, Raff JW, Prigent C, Glover DM (2002) Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules. The Journal of cell biology 156(3): 437-451
Giet R, Petretti C, Prigent C (2005) Aurora kinases, aneuploidy and cancer, a coincidence or a real link? Trends Cell Biol 15(5): 241-250
Giet R, Prigent C (1999) Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases. J Cell Sci 112 ( Pt 21): 3591-3601
Giet R, Uzbekov R, Cubizolles F, Le Guellec K, Prigent C (1999) The Xenopus laevis aurora-related protein kinase pEg2 associates with and phosphorylates the kinesin-related protein XlEg5. J Biol Chem 274(21): 15005-15013
Gillingham AK, Munro S (2000) The PACT domain, a conserved centrosomal targeting motif in the coiled-coil proteins AKAP450 and pericentrin. EMBO reports 1(6): 524-529
Glover DM, Hagan IM, Tavares AA (1998) Polo-like kinases: a team that plays throughout mitosis. Genes Dev 12(24): 3777-3787
Glover DM, Leibowitz MH, McLean DA, Parry H (1995) Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles. Cell 81(1): 95-105
Groisman I, Huang YS, Mendez R, Cao Q, Richter JD (2001) Translational control of embryonic cell division by CPEB and maskin. Cold Spring Harb Symp Quant Biol 66: 345-351
Gromley A, Jurczyk A, Sillibourne J, Halilovic E, Mogensen M, Groisman I, Blomberg M, Doxsey S (2003) A novel human protein of the maternal centriole is required for the final stages of cytokinesis and entry into S phase. The Journal of cell biology 161(3): 535-545
Habedanck R, Stierhof YD, Wilkinson CJ, Nigg EA (2005) The Polo kinase Plk4 functions in centriole duplication. Nat Cell Biol 7(11): 1140-1146
Hagan IM, Palazzo RE (2006) Warming up at the poles. EMBO reports 7(4): 364-371
Hahn WC, Stewart SA, Brooks MW, York SG, Eaton E, Kurachi A, Beijersbergen RL, Knoll JH, Meyerson M, Weinberg RA (1999) Inhibition of telomerase limits the growth of human cancer cells. Nat Med 5(10): 1164-1170
Hamanaka R, Maloid S, Smith MR, O'Connell CD, Longo DL, Ferris DK (1994) Cloning and characterization of human and murine homologues of the Drosophila polo serine-threonine kinase. Cell Growth Differ 5(3): 249-257
Hannak E, Kirkham M, Hyman AA, Oegema K (2001) Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans. The Journal of cell biology 155(7): 1109-1116
Hannak E, Oegema K, Kirkham M, Gonczy P, Habermann B, Hyman AA (2002) The kinetically dominant assembly pathway for centrosomal asters in Caenorhabditis elegans is gamma-tubulin dependent. The Journal of cell biology 157(4): 591-602
Heald R, Tournebize R, Blank T, Sandaltzopoulos R, Becker P, Hyman A, Karsenti E (1996) Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382(6590): 420-425
Helps NR, Luo X, Barker HM, Cohen PT (2000) NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase localized to centrosomes, is complexed to protein phosphatase 1. The Biochemical journal 349(Pt 2): 509-518
Herbert B, Pitts AE, Baker SI, Hamilton SE, Wright WE, Shay JW, Corey DR (1999) Inhibition of human telomerase in immortal human cells leads to progressive telomere shortening and cell death. Proceedings of the National Academy of Sciences of the United States of America 96(25): 14276-14281
Hinchcliffe EH, Miller FJ, Cham M, Khodjakov A, Sluder G (2001) Requirement of a centrosomal activity for cell cycle progression through G1 into S phase. Science (New York, NY 291(5508): 1547-1550
Hirota T, Kunitoku N, Sasayama T, Marumoto T, Zhang D, Nitta M, Hatakeyama K, Saya H (2003) Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells. Cell 114(5): 585-598
Holmfeldt P, Stenmark S, Gullberg M (2004) Differential functional interplay of TOGp/XMAP215 and the KinI kinesin MCAK during interphase and mitosis. The EMBO journal 23(3): 627-637
Howard J, Hyman AA (2003) Dynamics and mechanics of the microtubule plus end. Nature 422(6933): 753-758
Huang J, Raff JW (1999) The disappearance of cyclin B at the end of mitosis is regulated spatially in Drosophila cells. The EMBO journal 18(8): 2184-2195
Iarmarcovai G, Botta A, Orsiere T (2006) Number of centromeric signals in micronuclei and mechanisms of aneuploidy. Toxicol Lett 166(1): 1-10
Jackman M, Lindon C, Nigg EA, Pines J (2003) Active cyclin B1-Cdk1 first appears on centrosomes in prophase. Nat Cell Biol 5(2): 143-148
Jang YJ, Ma S, Terada Y, Erikson RL (2002) Phosphorylation of threonine 210 and the role of serine 137 in the regulation of mammalian polo-like kinase. J Biol Chem 277(46): 44115-44120
Job D, Valiron O, Oakley B (2003) Microtubule nucleation. Curr Opin Cell Biol 15(1): 111-117
Karsenti E, Vernos I (2001) The mitotic spindle: a self-made machine. Science (New York, NY 294(5542): 543-547
Katayama H, Brinkley WR, Sen S (2003) The Aurora kinases: role in cell transformation and tumorigenesis. Cancer Metastasis Rev 22(4): 451-464
Katayama H, Sasai K, Kawai H, Yuan ZM, Bondaruk J, Suzuki F, Fujii S, Arlinghaus RB, Czerniak BA, Sen S (2004) Phosphorylation by aurora kinase A induces Mdm2-mediated destabilization and inhibition of p53. Nat Genet 36(1): 55-62
Kemp CA, Kopish KR, Zipperlen P, Ahringer J, O'Connell KF (2004) Centrosome maturation and duplication in C. elegans require the coiled-coil protein SPD-2. Dev Cell 6(4): 511-523
Keryer G, Witczak O, Delouvee A, Kemmner WA, Rouillard D, Tasken K, Bornens M (2003) Dissociating the centrosomal matrix protein AKAP450 from centrioles impairs centriole duplication and cell cycle progression. Mol Biol Cell 14(6): 2436-2446
Khodjakov A, Cole RW, Oakley BR, Rieder CL (2000) Centrosome-independent mitotic spindle formation in vertebrates. Curr Biol 10(2): 59-67
Khodjakov A, Rieder CL (1999) The sudden recruitment of gamma-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules. The Journal of cell biology 146(3): 585-596
Khodjakov A, Rieder CL (2001) Centrosomes enhance the fidelity of cytokinesis in vertebrates and are required for cell cycle progression. The Journal of cell biology 153(1): 237-242
Khodjakov A, Rieder CL, Sluder G, Cassels G, Sibon O, Wang CL (2002) De novo formation of centrosomes in vertebrate cells arrested during S phase. The Journal of cell biology 158(7): 1171-1181
Kim SH, Kaminker P, Campisi J (1999) TIN2, a new regulator of telomere length in human cells. Nat Genet 23(4): 405-412
Kinoshita K, Habermann B, Hyman AA (2002) XMAP215: a key component of the dynamic microtubule cytoskeleton. Trends Cell Biol 12(6): 267-273
Kinoshita K, Noetzel TL, Pelletier L, Mechtler K, Drechsel DN, Schwager A, Lee M, Raff JW,Hyman AA (2005) Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis. The Journal of cell biology 170(7): 1047-1055
Kishi S, Wulf G, Nakamura M, Lu KP (2001) Telomeric protein Pin2/TRF1 induces mitotic entry and apoptosis in cells with short telomeres and is down-regulated in human breast tumors. Oncogene 20(12): 1497-1508
Kiyono T, Foster SA, Koop JI, McDougall JK, Galloway DA, Klingelhutz AJ (1998) Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 396(6706): 84-88
Kline-Smith SL, Walczak CE (2004) Mitotic spindle assembly and chromosome segregation: refocusing on microtubule dynamics. Mol Cell 15(3): 317-327
Klingelhutz AJ, Foster SA, McDougall JK (1996) Telomerase activation by the E6 gene product of human papillomavirus type 16. Nature 380(6569): 79-82
Kramer A, Mailand N, Lukas C, Syljuasen RG, Wilkinson CJ, Nigg EA, Bartek J, Lukas J (2004) Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase. Nat Cell Biol 6(9): 884-891
Kufer TA, Sillje HH, Korner R, Gruss OJ, Meraldi P, Nigg EA (2002) Human TPX2 is required for targeting Aurora-A kinase to the spindle. The Journal of cell biology 158(4): 617-623
Kunitoku N, Sasayama T, Marumoto T, Zhang D, Honda S, Kobayashi O, Hatakeyama K, Ushio Y, Saya H, Hirota T (2003) CENP-A phosphorylation by Aurora-A in prophase is required for enrichment of Aurora-B at inner centromeres and for kinetochore function. Dev Cell 5(6): 853-864
LaBranche H, Dupuis S, Ben-David Y, Bani MR, Wellinger RJ, Chabot B (1998) Telomere elongation by hnRNP A1 and a derivative that interacts with telomeric repeats and telomerase. Nat Genet 19(2): 199-202
Lacey KR, Jackson PK, Stearns T (1999) Cyclin-dependent kinase control of centrosome duplication. Proceedings of the National Academy of Sciences of the United States of America 96(6): 2817-2822
Lan W, Zhang X, Kline-Smith SL, Rosasco SE, Barrett-Wilt GA, Shabanowitz J, Hunt DF, Walczak CE, Stukenberg PT (2004) Aurora B phosphorylates centromeric MCAK and regulates its localization and microtubule depolymerization activity. Curr Biol 14(4): 273-286
Lanni JS, Jacks T (1998) Characterization of the p53-dependent postmitotic checkpoint following spindle disruption. Mol Cell Biol 18(2): 1055-1064
Le Bot N, Tsai MC, Andrews RK, Ahringer J (2003) TAC-1, a regulator of microtubule length inthe C. elegans embryo. Curr Biol 13(17): 1499-1505
Lee HW, Blasco MA, Gottlieb GJ, Horner JW, 2nd, Greider CW, DePinho RA (1998) Essential role of mouse telomerase in highly proliferative organs. Nature 392(6676): 569-574
Lee KS, Grenfell TZ, Yarm FR, Erikson RL (1998) Mutation of the polo-box disrupts localization and mitotic functions of the mammalian polo kinase Plk. Proceedings of the National Academy of Sciences of the United States of America 95(16): 9301-9306
Lee MJ, Gergely F, Jeffers K, Peak-Chew SY, Raff JW (2001) Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour. Nat Cell Biol 3(7): 643-649
Lin J, Blackburn EH (2004) Nucleolar protein PinX1p regulates telomerase by sequestering its protein catalytic subunit in an inactive complex lacking telomerase RNA. Genes Dev 18(4): 387-396
Lingle WL, Lutz WH, Ingle JN, Maihle NJ, Salisbury JL (1998) Centrosome hypertrophy in human breast tumors: implications for genomic stability and cell polarity. Proceedings of the National Academy of Sciences of the United States of America 95(6): 2950-2955
Liu Q, Kaneko S, Yang L, Feldman RI, Nicosia SV, Chen J, Cheng JQ (2004) Aurora-A abrogation of p53 DNA binding and transactivation activity by phosphorylation of serine 215. J Biol Chem 279(50): 52175-52182
Lu KP, Hanes SD, Hunter T (1996) A human peptidyl-prolyl isomerase essential for regulation of mitosis. Nature 380(6574): 544-547
Lustig AJ, Kurtz S, Shore D (1990) Involvement of the silencer and UAS binding protein RAP1 in regulation of telomere length. Science (New York, NY 250(4980): 549-553
Marumoto T, Honda S, Hara T, Nitta M, Hirota T, Kohmura E, Saya H (2003) Aurora-A kinase maintains the fidelity of early and late mitotic events in HeLa cells. J Biol Chem 278(51): 51786-51795
Matsumoto Y, Maller JL (2004) A centrosomal localization signal in cyclin E required for Cdk2-independent S phase entry. Science (New York, NY 306(5697): 885-888
Mayor T, Hacker U, Stierhof YD, Nigg EA (2002) The mechanism regulating the dissociation of the centrosomal protein C-Nap1 from mitotic spindle poles. J Cell Sci 115(Pt 16): 3275-3284
McEachern MJ, Krauskopf A, Blackburn EH (2000) Telomeres and their control. Annu Rev Genet 34: 331-358
Megraw TL, Kao LR, Kaufman TC (2001) Zygotic development without functional mitotic centrosomes. Curr Biol 11(2): 116-120
Mendez R, Richter JD (2001) Translational control by CPEB: a means to the end. Nature reviews 2(7): 521-529
Meraldi P, Honda R, Nigg EA (2002) Aurora-A overexpression reveals tetraploidization as a major route to centrosome amplification in p53-/- cells. The EMBO journal 21(4): 483-492
Meraldi P, Lukas J, Fry AM, Bartek J, Nigg EA (1999) Centrosome duplication in mammalian somatic cells requires E2F and Cdk2-cyclin A. Nat Cell Biol 1(2): 88-93
Moore A, Wordeman L (2004) The mechanism, function and regulation of depolymerizing kinesins during mitosis. Trends Cell Biol 14(10): 537-546
Nakamura M, Zhou XZ, Kishi S, Kosugi I, Tsutsui Y, Lu KP (2001) A specific interaction between the telomeric protein Pin2/TRF1 and the mitotic spindle. Curr Biol 11(19): 1512-1516
Nigg EA (2001) Mitotic kinases as regulators of cell division and its checkpoints. Nature reviews 2(1): 21-32
Nigg EA (2002) Centrosome aberrations: cause or consequence of cancer progression? Nature reviews 2(11): 815-825
Noetzel TL, Drechsel DN, Hyman AA, Kinoshita K (2005) A comparison of the ability of XMAP215 and tau to inhibit the microtubule destabilizing activity of XKCM1. Philosophical transactions of the Royal Society of London 360(1455): 591-594
Oh BK, Yoon SM, Lee CH, Park YN (2007) Rat homolog of PinX1 is a nucleolar protein involved in the regulation of telomere length. Gene 400(1-2): 35-43
Ohkura H, Garcia MA, Toda T (2001) Dis1/TOG universal microtubule adaptors - one MAP for all? J Cell Sci 114(Pt 21): 3805-3812
Okuda M, Horn HF, Tarapore P, Tokuyama Y, Smulian AG, Chan PK, Knudsen ES, Hofmann IA, Snyder JD, Bove KE, Fukasawa K (2000) Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication. Cell 103(1): 127-140
Pascreau G, Delcros JG, Cremet JY, Prigent C, Arlot-Bonnemains Y (2005) Phosphorylation of maskin by Aurora-A participates in the control of sequential protein synthesis during Xenopus laevis oocyte maturation. J Biol Chem 280(14): 13415-13423
Piehl M, Tulu US, Wadsworth P, Cassimeris L (2004) Centrosome maturation: measurement of microtubule nucleation throughout the cell cycle by using GFP-tagged EB1. Proceedings of the National Academy of Sciences of the United States of America 101(6): 1584-1588
Piel M, Nordberg J, Euteneuer U, Bornens M (2001) Centrosome-dependent exit of cytokinesis in animal cells. Science (New York, NY 291(5508): 1550-1553
Pihan GA, Purohit A, Wallace J, Knecht H, Woda B, Quesenberry P, Doxsey SJ (1998)
Centrosome defects and genetic instability in malignant tumors. Cancer Res 58(17): 3974-3985
Popov AV, Pozniakovsky A, Arnal I, Antony C, Ashford AJ, Kinoshita K, Tournebize R, Hyman AA, Karsenti E (2001) XMAP215 regulates microtubule dynamics through two distinctdomains. The EMBO journal 20(3): 397-410
Popov AV, Severin F, Karsenti E (2002) XMAP215 is required for the microtubule-nucleating activity of centrosomes. Curr Biol 12(15): 1326-1330
Prigozhina NL, Oakley CE, Lewis AM, Nayak T, Osmani SA, Oakley BR (2004) gamma-tubulin plays an essential role in the coordination of mitotic events. Mol Biol Cell 15(3): 1374-1386 Raff JW (2001) Centrosomes: Central no more? Curr Biol 11(5): R159-161
Raff JW (2002) Centrosomes and cancer: lessons from a TACC. Trends Cell Biol 12(5): 222-225
Raff JW, Jeffers K, Huang JY (2002) The roles of Fzy/Cdc20 and Fzr/Cdh1 in regulating the destruction of cyclin B in space and time. The Journal of cell biology 157(7): 1139-1149
Rajagopalan S, Bimbo A, Balasubramanian MK, Oliferenko S (2004) A potential tension-sensing mechanism that ensures timely anaphase onset upon metaphase spindle orientation. Curr Biol 14(1): 69-74
Reynolds N, Ohkura H (2003) Polo boxes form a single functional domain that mediates interactions with multiple proteins in fission yeast polo kinase. J Cell Sci 116(Pt 7): 1377-1387
Roghi C, Giet R, Uzbekov R, Morin N, Chartrain I, Le Guellec R, Couturier A, Doree M, Philippe M, Prigent C (1998) The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly. J Cell Sci 111 ( Pt 5): 557-572
Sampath SC, Ohi R, Leismann O, Salic A, Pozniakovski A, Funabiki H (2004) The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly. Cell 118(2): 187-202
Sankaran S, Parvin JD (2006) Centrosome function in normal and tumor cells. J Cell Biochem 99(5): 1240-1250
Shay JW, Wright WE (2001) Telomeres and telomerase: implications for cancer and aging. Radiat Res 155(1 Pt 2): 188-193
Shen M, Haggblom C, Vogt M, Hunter T, Lu KP (1997) Characterization and cell cycle regulation of the related human telomeric proteins Pin2 and TRF1 suggest a role in mitosis. Proceedings of the National Academy of Sciences of the United States of America 94(25): 13618-13623
Sherr CJ (1996) Cancer cell cycles. Science (New York, NY 274(5293): 1672-1677
Shimamura M, Brown RC, Lemmon BE, Akashi T, Mizuno K, Nishihara N, Tomizawa K, Yoshimoto K, Deguchi H, Hosoya H, Horio T, Mineyuki Y (2004) Gamma-tubulin in basal land plants: characterization, localization, and implication in the evolution of acentriolar microtubule organizing centers. Plant Cell 16(1): 45-59
Smith S, Giriat I, Schmitt A, de Lange T (1998) Tankyrase, a poly(ADP-ribose) polymerase athuman telomeres. Science (New York, NY 282(5393): 1484-1487
Smogorzewska A, van Steensel B, Bianchi A, Oelmann S, Schaefer MR, Schnapp G, de Lange T (2000) Control of human telomere length by TRF1 and TRF2. Mol Cell Biol 20(5): 1659-1668
Srayko M, Quintin S, Schwager A, Hyman AA (2003) Caenorhabditis elegans TAC-1 and ZYG-9 form a complex that is essential for long astral and spindle microtubules. Curr Biol 13(17): 1506-1511
Srsen V, Merdes A (2006) The centrosome and cell proliferation. Cell division 1: 26
Stebbins-Boaz B, Cao Q, de Moor CH, Mendez R, Richter JD (1999) Maskin is a CPEB-associated factor that transiently interacts with elF-4E. Mol Cell 4(6): 1017-1027
Stewart SA, Weinberg RA (2000) Telomerase and human tumorigenesis. Semin Cancer Biol 10(6): 399-406
Still IH, Vettaikkorumakankauv AK, DiMatteo A, Liang P (2004) Structure-function evolution of the transforming acidic coiled coil genes revealed by analysis of phylogenetically diverse organisms. BMC Evol Biol 4: 16
Taguchi S, Honda K, Sugiura K, Yamaguchi A, Furukawa K, Urano T (2002) Degradation of human Aurora-A protein kinase is mediated by hCdh1. FEBS Lett 519(1-3): 59-65
Takai N, Hamanaka R, Yoshimatsu J, Miyakawa I (2005) Polo-like kinases (Plks) and cancer. Oncogene 24(2): 287-291
Tatsuka M, Katayama H, Ota T, Tanaka T, Odashima S, Suzuki F, Terada Y (1998) Multinuclearity and increased ploidy caused by overexpression of the aurora- and Ipl1-like midbody-associated protein mitotic kinase in human cancer cells. Cancer Res 58(21): 4811-4816
Tavares AA, Glover DM, Sunkel CE (1996) The conserved mitotic kinase polo is regulated by phosphorylation and has preferred microtubule-associated substrates in Drosophila embryo extracts. The EMBO journal 15(18): 4873-4883
Terada Y, Uetake Y, Kuriyama R (2003) Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells. The Journal of cell biology 162(5): 757-763
Theurkauf WE, Hawley RS (1992) Meiotic spindle assembly in Drosophila females: behavior of nonexchange chromosomes and the effects of mutations in the nod kinesin-like protein. The Journal of cell biology 116(5): 1167-1180
Tokuyama Y, Horn HF, Kawamura K, Tarapore P, Fukasawa K (2001) Specific phosphorylation of nucleophosmin on Thr(199) by cyclin-dependent kinase 2-cyclin E and its role in centrosome duplication. J Biol Chem 276(24): 21529-21537
Tournebize R, Popov A, Kinoshita K, Ashford AJ, Rybina S, Pozniakovsky A, Mayer TU, WalczakCE, Karsenti E, Hyman AA (2000) Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts. Nat Cell Biol 2(1): 13-19
Trielli MO, Andreassen PR, Lacroix FB, Margolis RL (1996) Differential Taxol-dependent arrest of transformed and nontransformed cells in the G1 phase of the cell cycle, and specific-related mortality of transformed cells. The Journal of cell biology 135(3): 689-700
Tsai MY, Wiese C, Cao K, Martin O, Donovan P, Ruderman J, Prigent C, Zheng Y (2003) A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly. Nat Cell Biol 5(3): 242-248
Tsou MF, Stearns T (2006) Controlling centrosome number: licenses and blocks. Curr Opin Cell Biol 18(1): 74-78
Uetake Y, Sluder G (2004) Cell cycle progression after cleavage failure: mammalian somatic cells do not possess a "tetraploidy checkpoint". The Journal of cell biology 165(5): 609-615
Usui T, Maekawa H, Pereira G, Schiebel E (2003) The XMAP215 homologue Stu2 at yeast spindle pole bodies regulates microtubule dynamics and anchorage. The EMBO journal 22(18): 4779-4793
van Overbeek M, de Lange T (2006) Apollo, an Artemis-related nuclease, interacts with TRF2 and protects human telomeres in S phase. Curr Biol 16(13): 1295-1302
van Steensel B, de Lange T (1997) Control of telomere length by the human telomeric protein TRF1. Nature 385(6618): 740-743
Vandre DD, Borisy GG (1989) Anaphase onset and dephosphorylation of mitotic phosphoproteins occur concomitantly. J Cell Sci 94 ( Pt 2): 245-258
Vaziri H, Benchimol S (1998) Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span. Curr Biol 8(5): 279-282
Verde F, Dogterom M, Stelzer E, Karsenti E, Leibler S (1992) Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts. The Journal of cell biology 118(5): 1097-1108
Wakefield JG, Huang JY, Raff JW (2000) Centrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos. Curr Biol 10(21): 1367-1370
Walczak CE, Mitchison TJ, Desai A (1996) XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly. Cell 84(1): 37-47
Walker RA, O'Brien ET, Pryer NK, Soboeiro MF, Voter WA, Erickson HP, Salmon ED (1988) Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies. The Journal of cell biology 107(4): 1437-1448
Wang J, Xie LY, Allan S, Beach D, Hannon GJ (1998) Myc activates telomerase. Genes Dev12(12): 1769-1774
Warnke S, Kemmler S, Hames RS, Tsai HL, Hoffmann-Rohrer U, Fry AM, Hoffmann I (2004) Polo-like kinase-2 is required for centriole duplication in mammalian cells. Curr Biol 14(13): 1200-1207
Wittmann T, Hyman A, Desai A (2001) The spindle: a dynamic assembly of microtubules and motors. Nat Cell Biol 3(1): E28-34
Wong C, Stearns T (2005) Mammalian cells lack checkpoints for tetraploidy, aberrant centrosome number, and cytokinesis failure. BMC Cell Biol 6(1): 6
Wu KJ, Grandori C, Amacker M, Simon-Vermot N, Polack A, Lingner J, Dalla-Favera R (1999) Direct activation of TERT transcription by c-MYC. Nat Genet 21(2): 220-224
Yoo JE, Oh BK, Park YN (2009) Human PinX1 Mediates TRF1 Accumulation in Nucleolus and Enhances TRF1 Binding to Telomeres. J Mol Biol
Young AC, Chavez M, Giambernardi TA, Mattern V, McGill JR, Harris JM, Sarosdy MF, Patel P, Sakaguchi AY (1997) Organization and expression of human telomere repeat binding factor genes. Somat Cell Mol Genet 23(4): 275-286
Zhang X, Mar V, Zhou W, Harrington L, Robinson MO (1999) Telomere shortening and apoptosis in telomerase-inhibited human tumor cells. Genes Dev 13(18): 2388-2399
Zhou J, Monson EK, Teng SC, Schulz VP, Zakian VA (2000) Pif1p helicase, a catalytic inhibitor of telomerase in yeast. Science (New York, NY 289(5480): 771-774
Zhou XZ, Lu KP (2001) The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor. Cell 107(3): 347-359
Zhu XD, Niedernhofer L, Kuster B, Mann M, Hoeijmakers JH, de Lange T (2003) ERCC1/XPF removes the 3' overhang from uncapped telomeres and represses formation of telomeric DNA-containing double minute chromosomes. Mol Cell 12(6): 1489-1498
Zimmerman WC, Sillibourne J, Rosa J, Doxsey SJ (2004) Mitosis-specific anchoring of gamma tubulin complexes by pericentrin controls spindle organization and mitotic entry. Mol Biol Cell 15(8): 3642-3657
Barros TP, Kinoshita K, Hyman AA, Raff JW (2005) Aurora A activates D-TACC-Msps complexes exclusively at centrosomes to stabilize centrosomal microtubules. The Journal of cell biology 170(7): 1039-1046
Chen HM, Schmeichel KL, Mian IS, Lelievre S, Petersen OW, Bissell MJ (2000) AZU-1: a candidate breast tumor suppressor and biomarker for tumor progression. Mol Biol Cell 11(4): 1357-1367
D'Assoro AB, Lingle WL, Salisbury JL (2002) Centrosome amplification and the development of cancer. Oncogene 21(40): 6146-6153
Doxsey S, Zimmerman W, Mikule K (2005) Centrosome control of the cell cycle. Trends Cell Biol 15(6): 303-311
Gergely F (2002) Centrosomal TACCtics. Bioessays 24(10): 915-925
Gergely F, Karlsson C, Still I, Cowell J, Kilmartin J, Raff JW (2000) The TACC domain identifies a family of centrosomal proteins that can interact with microtubules. Proceedings of the National Academy of Sciences of the United States of America 97(26): 14352-14357
Giet R, McLean D, Descamps S, Lee MJ, Raff JW, Prigent C, Glover DM (2002) Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules. The Journal of cell biology 156(3): 437-451
Harrington EA, Bebbington D, Moore J, Rasmussen RK, Ajose-Adeogun AO, Nakayama T, Graham JA, Demur C, Hercend T, Diu-Hercend A, Su M, Golec JM, Miller KM (2004) VX-680, a potent and selective small-molecule inhibitor of the Aurora kinases, suppresses tumor growth in vivo. Nat Med 10(3): 262-267
Iarmarcovai G, Botta A, Orsiere T (2006) Number of centromeric signals in micronuclei and mechanisms of aneuploidy. Toxicol Lett 166(1): 1-10
Kinoshita K, Noetzel TL, Pelletier L, Mechtler K, Drechsel DN, Schwager A, Lee M, Raff JW, Hyman AA (2005) Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis. The Journal of cell biology 170(7): 1047-1055
Lauffart B, Gangisetty O, Still IH (2003) Molecular cloning, genomic structure and interactions of the putative breast tumor suppressor TACC2. Genomics 81(2): 192-201
LeRoy PJ, Hunter JJ, Hoar KM, Burke KE, Shinde V, Ruan J, Bowman D, Galvin K, Ecsedy JA (2007) Localization of human TACC3 to mitotic spindles is mediated by phosphorylation on Ser558 by Aurora A: a novel pharmacodynamic method for measuring Aurora A activity.Cancer Res 67(11): 5362-5370
Pascreau G, Delcros JG, Cremet JY, Prigent C, Arlot-Bonnemains Y (2005) Phosphorylation of maskin by Aurora-A participates in the control of sequential protein synthesis during Xenopus laevis oocyte maturation. J Biol Chem 280(14): 13415-13423
Peset I, Seiler J, Sardon T, Bejarano LA, Rybina S, Vernos I (2005) Function and regulation of Maskin, a TACC family protein, in microtubule growth during mitosis. The Journal of cell biology 170(7): 1057-1066
Raff JW (2002) Centrosomes and cancer: lessons from a TACC. Trends Cell Biol 12(5): 222-225
Schuendeln MM, Piekorz RP, Wichmann C, Lee Y, McKinnon PJ, Boyd K, Takahashi Y, Ihle JN (2004) The centrosomal, putative tumor suppressor protein TACC2 is dispensable for normal development, and deficiency does not lead to cancer. Mol Cell Biol 24(14): 6403-6409
Srsen V, Merdes A (2006) The centrosome and cell proliferation. Cell division 1: 26 Still IH, Vettaikkorumakankauv AK, DiMatteo A, Liang P (2004) Structure-function evolution of the transforming acidic coiled coil genes revealed by analysis of phylogenetically diverse organisms. BMC Evol Biol 4: 16
Tien AC, Lin MH, Su LJ, Hong YR, Cheng TS, Lee YC, Lin WJ, Still IH, Huang CY (2004) Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model. Mol Cell Proteomics 3(1): 93-104
Banik SS, Counter CM (2004) Characterization of interactions between PinX1 and human telomerase subunits hTERT and hTR. J Biol Chem 279(50): 51745-51748
Blower MD, Nachury M, Heald R, Weis K (2005) A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. Cell 121(2): 223-234
Chan GK, Jablonski SA, Starr DA, Goldberg ML, Yen TJ (2000) Human Zw10 and ROD are mitotic checkpoint proteins that bind to kinetochores. Nat Cell Biol 2(12): 944-947
Cheeseman IM, Chappie JS, Wilson-Kubalek EM, Desai A (2006) The conserved KMN network constitutes the core microtubule-binding site of the kinetochore. Cell 127(5): 983-997
DeLuca JG, Gall WE, Ciferri C, Cimini D, Musacchio A, Salmon ED (2006) Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. Cell 127(5): 969-982
Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR, 3rd, Cleveland DW (2006) The human CENP-A centromeric nucleosome-associated complex. Nat Cell Biol 8(5): 458-469
Fujita Y, Hayashi T, Kiyomitsu T, Toyoda Y, Kokubu A, Obuse C, Yanagida M (2007) Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1. Dev Cell 12(1): 17-30
Guglielmi B, Werner M (2002) The yeast homolog of human PinX1 is involved in rRNA and small nucleolar RNA maturation, not in telomere elongation inhibition. J Biol Chem 277(38): 35712-35719
Heald R, Tournebize R, Blank T, Sandaltzopoulos R, Becker P, Hyman A, Karsenti E (1996) Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382(6590): 420-425
Kalab P, Weis K, Heald R (2002) Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts. Science (New York, NY 295(5564): 2452-2456
Lin J, Blackburn EH (2004) Nucleolar protein PinX1p regulates telomerase by sequestering its protein catalytic subunit in an inactive complex lacking telomerase RNA. Genes Dev 18(4): 387-396
Lin J, Jin R, Zhang B, Yang PX, Chen H, Bai YX, Xie Y, Huang C, Huang J (2007) Characterization of a novel effect of hPinX1 on hTERT nucleolar localization. Biochem Biophys Res Commun 353(4): 946-952
Liu D, Ding X, Du J, Cai X, Huang Y, Ward T, Shaw A, Yang Y, Hu R, Jin C, Yao X (2007) Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment. J Biol Chem 282(29): 21415-21424
Lou Y, Yao J, Zereshki A, Dou Z, Ahmed K, Wang H, Hu J, Wang Y, Yao X (2004) NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling. J Biol Chem 279(19): 20049-20057
Ma N, Matsunaga S, Takata H, Ono-Maniwa R, Uchiyama S, Fukui K (2007) Nucleolin functions in nucleolus formation and chromosome congression. J Cell Sci 120(Pt 12): 2091-2105
Mao Y, Desai A, Cleveland DW (2005) Microtubule capture by CENP-E silences BubR1-dependent mitotic checkpoint signaling. The Journal of cell biology 170(6): 873-880
Mazumdar M, Misteli T (2005) Chromokinesins: multitalented players in mitosis. Trends Cell Biol 15(7): 349-355
McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ, Stukenberg PT (2003) The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev 17(1): 101-114
McCleland ML, Kallio MJ, Barrett-Wilt GA, Kestner CA, Shabanowitz J, Hunt DF, Gorbsky GJ, Stukenberg PT (2004) The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are required to establish and maintain kinetochore-microtubule attachment. Curr Biol 14(2): 131-137
Musacchio A, Salmon ED (2007) The spindle-assembly checkpoint in space and time. Nature reviews 8(5): 379-393
Nachury MV, Maresca TJ, Salmon WC, Waterman-Storer CM, Heald R, Weis K (2001) Importin beta is a mitotic target of the small GTPase Ran in spindle assembly. Cell 104(1): 95-106 Nakamura M, Zhou XZ, Kishi S, Kosugi I, Tsutsui Y, Lu KP (2001) A specific interaction between the telomeric protein Pin2/TRF1 and the mitotic spindle. Curr Biol 11(19): 1512-1516
Oh BK, Yoon SM, Lee CH, Park YN (2007) Rat homolog of PinX1 is a nucleolar protein involved in the regulation of telomere length. Gene 400(1-2): 35-43
Rieder CL (1982) The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber. Int Rev Cytol 79: 1-58
Sakita-Suto S, Kanda A, Suzuki F, Sato S, Takata T, Tatsuka M (2007) Aurora-B Regulates RNA Methyltransferase NSUN2. Mol Biol Cell
Tahara K, Takagi M, Ohsugi M, Sone T, Nishiumi F, Maeshima K, Horiuchi Y, Tokai-Nishizumi N, Imamoto F, Yamamoto T, Kose S, Imamoto N (2008) Importin-beta and the small guanosine triphosphatase Ran mediate chromosome loading of the human chromokinesin Kid. The Journal of cell biology 180(3): 493-506
Uchiyama S, Kobayashi S, Takata H, Ishihara T, Hori N, Higashi T, Hayashihara K, Sone T, Higo D, Nirasawa T, Takao T, Matsunaga S, Fukui K (2005) Proteome analysis of human metaphasechromosomes. J Biol Chem 280(17): 16994-17004
Van Hooser AA, Yuh P, Heald R (2005) The perichromosomal layer. Chromosoma 114(6): 377-388
Vergnolle MA, Taylor SS (2007) Cenp-F Links Kinetochores to Ndel1/Nde1/Lis1/Dynein Microtubule Motor Complexes. Curr Biol
Wang H, Hu X, Ding X, Dou Z, Yang Z, Shaw AW, Teng M, Cleveland DW, Goldberg ML, Niu L, Yao X (2004) Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling. J Biol Chem 279(52): 54590-54598
Wei RR, Al-Bassam J, Harrison SC (2007) The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment. Nat Struct Mol Biol 14(1): 54-59
Wigge PA, Kilmartin JV (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. The Journal of cell biology 152(2): 349-360
Wright K, Wilson PJ, Kerr J, Do K, Hurst T, Khoo SK, Ward B, Chenevix-Trench G (1998) Frequent loss of heterozygosity and three critical regions on the short arm of chromosome 8 in ovarian adenocarcinomas. Oncogene 17(9): 1185-1188
Yang Y, Wu F, Ward T, Yan F, Wu Q, Wang Z, McGlothen T, Peng W, You T, Cui T, Hu R, Dou Z, Zhu J, Xie W, Rao Z, Yao X (2008) Phosphorylation of HsMis13 by aurora B is essential for assembly of functional kinetochore. J Biol Chem
Yao X, Abrieu A, Zheng Y, Sullivan KF, Cleveland DW (2000) CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint. Nat Cell Biol 2(8): 484-491
Yao X, Anderson KL, Cleveland DW (1997) The microtubule-dependent motor centromere-associated protein E (CENP-E) is an integral component of kinetochore corona fibers that link centromeres to spindle microtubules. The Journal of cell biology 139(2): 435-447
Zhou XZ, Lu KP (2001) The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor. Cell 107(3): 347-359
Banik SS, Counter CM (2004) Characterization of interactions between PinX1 and human telomerase subunits hTERT and hTR. J Biol Chem 279(50): 51745-51748
Bertuch AA, Lundblad V (2003) The Ku heterodimer performs separable activities at double-strand breaks and chromosome termini. Mol Cell Biol 23(22): 8202-8215
Cao X, Ding X, Guo Z, Zhou R, Wang F, Long F, Wu F, Bi F, Wang Q, Fan D, Forte JG, Teng M, Yao X (2005) PALS1 specifies the localization of ezrin to the apical membrane of gastric parietal cells. J Biol Chem 280(14): 13584-13592
Dundr M, Meier UT, Lewis N, Rekosh D, Hammarskjold ML, Olson MO (1997) A class of nonribosomal nucleolar components is located in chromosome periphery and in nucleolus-derived foci during anaphase and telophase. Chromosoma 105(7-8): 407-417
Earnshaw WC, Halligan B, Cooke CA, Heck MM, Liu LF (1985) Topoisomerase II is a structural component of mitotic chromosome scaffolds. The Journal of cell biology 100(5): 1706-1715
Gautier T, Masson C, Quintana C, Arnoult J, Hernandez-Verdun D (1992) The ultrastructure of the chromosome periphery in human cell lines. An in situ study using cryomethods in electron microscopy. Chromosoma 101(8): 502-510
Hanakahi LA, Sun H, Maizels N (1999) High affinity interactions of nucleolin with G-G-paired rDNA. J Biol Chem 274(22): 15908-15912
Heald R, Tournebize R, Blank T, Sandaltzopoulos R, Becker P, Hyman A, Karsenti E (1996) Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382(6590): 420-425
Hernandez-Verdun D, Gautier T (1994) The chromosome periphery during mitosis. Bioessays 16(3): 179-185
Hood FE, Clarke PR (2007) RCC1 isoforms differ in their affinity for chromatin, molecular interactions and regulation by phosphorylation. J Cell Sci 120(Pt 19): 3436-3445
Lee YM, Lee S, Lee E, Shin H, Hahn H, Choi W, Kim W (2001) Human kinesin superfamily member 4 is dominantly localized in the nuclear matrix and is associated with chromosomes during mitosis. The Biochemical journal 360(Pt 3): 549-556
Lin J, Blackburn EH (2004) Nucleolar protein PinX1p regulates telomerase by sequestering its protein catalytic subunit in an inactive complex lacking telomerase RNA. Genes Dev 18(4): 387-396
Lin J, Jin R, Zhang B, Yang PX, Chen H, Bai YX, Xie Y, Huang C, Huang J (2007) Characterization of a novel effect of hPinX1 on hTERT nucleolar localization. BiochemBiophys Res Commun 353(4): 946-952
Ma N, Matsunaga S, Takata H, Ono-Maniwa R, Uchiyama S, Fukui K (2007) Nucleolin functions in nucleolus formation and chromosome congression. J Cell Sci 120(Pt 12): 2091-2105
McGill M, Brinkley BR (1975) Human chromosomes and centrioles as nucleating sites for the in vitro assembly of microtubules from bovine brain tubulin. The Journal of cell biology 67(1): 189-199
Mitchison TJ, Kirschner MW (1985) Properties of the kinetochore in vitro. I. Microtubule nucleation and tubulin binding. The Journal of cell biology 101(3): 755-765
Sharp LW (1929) Structure of large somatic chromosomes. Bot Gaz 88: 349-382
Srivastava M, Pollard HB (1999) Molecular dissection of nucleolin's role in growth and cell proliferation: new insights. Faseb J 13(14): 1911-1922
Takagi M, Matsuoka Y, Kurihara T, Yoneda Y (1999) Chmadrin: a novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure. J Cell Sci 112 ( Pt 15): 2463-2472
Takata H, Matsunaga S, Morimoto A, Ono-Maniwa R, Uchiyama S, Fukui K (2007) H1.X with different properties from other linker histones is required for mitotic progression. FEBS Lett 581(20): 3783-3788
Van Hooser AA, Yuh P, Heald R (2005) The perichromosomal layer. Chromosoma 114(6): 377-388
Wollman R, Cytrynbaum EN, Jones JT, Meyer T, Scholey JM, Mogilner A (2005) Efficient chromosome capture requires a bias in the 'search-and-capture' process during mitotic-spindle assembly. Curr Biol 15(9): 828-832
Yajima J, Edamatsu M, Watai-Nishii J, Tokai-Nishizumi N, Yamamoto T, Toyoshima YY (2003) The human chromokinesin Kid is a plus end-directed microtubule-based motor. The EMBO journal 22(5): 1067-1074
Yuan K, Hu H, Guo Z, Fu G, Shaw AP, Hu R, Yao X (2007) Phospho-regulation of HsCdc14A By Polo-like kinase 1 is essential for mitotic progression. J Biol Chem 282(37): 27414-27423
Zhou XZ, Lu KP (2001) The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor. Cell 107(3): 347-359
Andreassen PR, Lohez OD, Lacroix FB, Margolis RL (2001) Tetraploid state induces p53-dependent arrest of nontransformed mammalian cells in G1. Mol Biol Cell 12(5): 1315-1328
Andrews PD, Knatko E, Moore WJ, Swedlow JR (2003) Mitotic mechanics: the auroras come into view. Curr Opin Cell Biol 15(6): 672-683
Andrews PD, Ovechkina Y, Morrice N, Wagenbach M, Duncan K, Wordeman L, Swedlow JR (2004) Aurora B regulates MCAK at the mitotic centromere. Dev Cell 6(2): 253-268
Artandi SE, DePinho RA (2000) A critical role for telomeres in suppressing and facilitating carcinogenesis. Curr Opin Genet Dev 10(1): 39-46
Barros TP, Kinoshita K, Hyman AA, Raff JW (2005) Aurora A activates D-TACC-Msps complexes exclusively at centrosomes to stabilize centrosomal microtubules. The Journal of cell biology 170(7): 1039-1046
Bellanger JM, Gonczy P (2003) TAC-1 and ZYG-9 form a complex that promotes microtubule assembly in C. elegans embryos. Curr Biol 13(17): 1488-1498
Berdnik D, Knoblich JA (2002) Drosophila Aurora-A is required for centrosome maturation and actin-dependent asymmetric protein localization during mitosis. Curr Biol 12(8): 640-647
Bianchi A, Smith S, Chong L, Elias P, de Lange T (1997) TRF1 is a dimer and bends telomeric DNA. The EMBO journal 16(7): 1785-1794
Bischoff JR, Anderson L, Zhu Y, Mossie K, Ng L, Souza B, Schryver B, Flanagan P, Clairvoyant F, Ginther C, Chan CS, Novotny M, Slamon DJ, Plowman GD (1998) A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers. The EMBO journal 17(11): 3052-3065
Blasco MA, Lee HW, Hande MP, Samper E, Lansdorp PM, DePinho RA, Greider CW (1997) Telomere shortening and tumor formation by mouse cells lacking telomerase RNA. Cell 91(1): 25-34
Bobinnec Y, Khodjakov A, Mir LM, Rieder CL, Edde B, Bornens M (1998) Centriole disassembly in vivo and its effect on centrosome structure and function in vertebrate cells. The Journal of cell biology 143(6): 1575-1589
Bodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu CP, Morin GB, Harley CB, Shay JW, Lichtsteiner S, Wright WE (1998) Extension of life-span by introduction of telomerase intonormal human cells. Science (New York, NY 279(5349): 349-352
Cheeseman IM, Anderson S, Jwa M, Green EM, Kang J, Yates JR, 3rd, Chan CS, Drubin DG, Barnes G (2002) Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p. Cell 111(2): 163-172
Chen Y, Yang Y, van Overbeek M, Donigian JR, Baciu P, de Lange T, Lei M (2008) A Shared Docking Motif in TRF1 and TRF2 Used for Differential Recruitment of Telomeric Proteins. Science (New York, NY
Chen Z, Indjeian VB, McManus M, Wang L, Dynlacht BD (2002) CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells. Dev Cell 3(3): 339-350
Conrad MN, Wright JH, Wolf AJ, Zakian VA (1990) RAP1 protein interacts with yeast telomeres in vivo: overproduction alters telomere structure and decreases chromosome stability. Cell 63(4): 739-750
Conte N, Delaval B, Ginestier C, Ferrand A, Isnardon D, Larroque C, Prigent C, Seraphin B, Jacquemier J, Birnbaum D (2003) TACC1-chTOG-Aurora A protein complex in breast cancer. Oncogene 22(50): 8102-8116
Cooper JP, Nimmo ER, Allshire RC, Cech TR (1997) Regulation of telomere length and function by a Myb-domain protein in fission yeast. Nature 385(6618): 744-747
Cullen CF, Ohkura H (2001) Msps protein is localized to acentrosomal poles to ensure bipolarity of Drosophila meiotic spindles. Nat Cell Biol 3(7): 637-642
de Saint Phalle B, Sullivan W (1998) Spindle assembly and mitosis without centrosomes in parthenogenetic Sciara embryos. The Journal of cell biology 141(6): 1383-1391
Desai A, Verma S, Mitchison TJ, Walczak CE (1999) Kin I kinesins are microtubule-destabilizing enzymes. Cell 96(1): 69-78
Diviani D, Scott JD (2001) AKAP signaling complexes at the cytoskeleton. J Cell Sci 114(Pt 8): 1431-1437
Doxsey S, Zimmerman W, Mikule K (2005) Centrosome control of the cell cycle. Trends Cell Biol 15(6): 303-311
Du J, Hannon GJ (2004) Suppression of p160ROCK bypasses cell cycle arrest after Aurora-A/STK15 depletion. Proceedings of the National Academy of Sciences of the United States of America 101(24): 8975-8980
Dutertre S, Descamps S, Prigent C (2002) On the role of aurora-A in centrosome function. Oncogene 21(40): 6175-6183
Dutertre S, Hamard-Peron E, Cremet JY, Thomas Y, Prigent C (2005) The absence of p53 aggravates polyploidy and centrosome number abnormality induced by Aurora-Coverexpression. Cell cycle (Georgetown, Tex 4(12): 1783-1787
Elliott S, Knop M, Schlenstedt G, Schiebel E (1999) Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication. Proceedings of the National Academy of Sciences of the United States of America 96(11): 6205-6210
Eto M, Elliott E, Prickett TD, Brautigan DL (2002) Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation. J Biol Chem 277(46): 44013-44020
Evans SK, Lundblad V (1999) Est1 and Cdc13 as comediators of telomerase access. Science (New York, NY 286(5437): 117-120
Eyers PA, Erikson E, Chen LG, Maller JL (2003) A novel mechanism for activation of the protein kinase Aurora A. Curr Biol 13(8): 691-697
Fairall L, Chapman L, Moss H, de Lange T, Rhodes D (2001) Structure of the TRFH dimerization domain of the human telomeric proteins TRF1 and TRF2. Mol Cell 8(2): 351-361
Feng J, Funk WD, Wang SS, Weinrich SL, Avilion AA, Chiu CP, Adams RR, Chang E, Allsopp RC, Yu J, et al. (1995) The RNA component of human telomerase. Science (New York, NY 269(5228): 1236-1241
Fry AM, Mayor T, Meraldi P, Stierhof YD, Tanaka K, Nigg EA (1998a) C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2. The Journal of cell biology 141(7): 1563-1574
Fry AM, Meraldi P, Nigg EA (1998b) A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators. The EMBO journal 17(2): 470-481
Fujiwara T, Bandi M, Nitta M, Ivanova EV, Bronson RT, Pellman D (2005) Cytokinesis failure generating tetraploids promotes tumorigenesis in p53-null cells. Nature 437(7061): 1043-1047
Gadde S, Heald R (2004) Mechanisms and molecules of the mitotic spindle. Curr Biol 14(18): R797-805
Gadea BB, Ruderman JV (2006) Aurora B is required for mitotic chromatin-induced phosphorylation of Op18/Stathmin. Proceedings of the National Academy of Sciences of the United States of America 103(12): 4493-4498
Gard DL, Becker BE, Josh Romney S (2004) MAPping the eukaryotic tree of life: structure, function, and evolution of the MAP215/Dis1 family of microtubule-associated proteins. Int Rev Cytol 239: 179-272
Gard DL, Kirschner MW (1987) A microtubule-associated protein from Xenopus eggs that specifically promotes assembly at the plus-end. The Journal of cell biology 105(5): 2203-2215
Gergely F (2002) Centrosomal TACCtics. Bioessays 24(10): 915-925
Gergely F, Draviam VM, Raff JW (2003) The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells. Genes Dev 17(3): 336-341
Gergely F, Karlsson C, Still I, Cowell J, Kilmartin J, Raff JW (2000) The TACC domain identifies a family of centrosomal proteins that can interact with microtubules. Proceedings of the National Academy of Sciences of the United States of America 97(26): 14352-14357
Gergely F, Kidd D, Jeffers K, Wakefield JG, Raff JW (2000) D-TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo. The EMBO journal 19(2): 241-252
Giansanti MG, Gatti M, Bonaccorsi S (2001) The role of centrosomes and astral microtubules during asymmetric division of Drosophila neuroblasts. Development 128(7): 1137-1145
Giet R, McLean D, Descamps S, Lee MJ, Raff JW, Prigent C, Glover DM (2002) Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules. The Journal of cell biology 156(3): 437-451
Giet R, Petretti C, Prigent C (2005) Aurora kinases, aneuploidy and cancer, a coincidence or a real link? Trends Cell Biol 15(5): 241-250
Giet R, Prigent C (1999) Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases. J Cell Sci 112 ( Pt 21): 3591-3601
Giet R, Uzbekov R, Cubizolles F, Le Guellec K, Prigent C (1999) The Xenopus laevis aurora-related protein kinase pEg2 associates with and phosphorylates the kinesin-related protein XlEg5. J Biol Chem 274(21): 15005-15013
Gillingham AK, Munro S (2000) The PACT domain, a conserved centrosomal targeting motif in the coiled-coil proteins AKAP450 and pericentrin. EMBO reports 1(6): 524-529
Glover DM, Hagan IM, Tavares AA (1998) Polo-like kinases: a team that plays throughout mitosis. Genes Dev 12(24): 3777-3787
Glover DM, Leibowitz MH, McLean DA, Parry H (1995) Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles. Cell 81(1): 95-105
Groisman I, Huang YS, Mendez R, Cao Q, Richter JD (2001) Translational control of embryonic cell division by CPEB and maskin. Cold Spring Harb Symp Quant Biol 66: 345-351
Gromley A, Jurczyk A, Sillibourne J, Halilovic E, Mogensen M, Groisman I, Blomberg M, Doxsey S (2003) A novel human protein of the maternal centriole is required for the final stages of cytokinesis and entry into S phase. The Journal of cell biology 161(3): 535-545
Habedanck R, Stierhof YD, Wilkinson CJ, Nigg EA (2005) The Polo kinase Plk4 functions in centriole duplication. Nat Cell Biol 7(11): 1140-1146
Hagan IM, Palazzo RE (2006) Warming up at the poles. EMBO reports 7(4): 364-371
Hahn WC, Stewart SA, Brooks MW, York SG, Eaton E, Kurachi A, Beijersbergen RL, Knoll JH, Meyerson M, Weinberg RA (1999) Inhibition of telomerase limits the growth of human cancer cells. Nat Med 5(10): 1164-1170
Hamanaka R, Maloid S, Smith MR, O'Connell CD, Longo DL, Ferris DK (1994) Cloning and characterization of human and murine homologues of the Drosophila polo serine-threonine kinase. Cell Growth Differ 5(3): 249-257
Hannak E, Kirkham M, Hyman AA, Oegema K (2001) Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans. The Journal of cell biology 155(7): 1109-1116
Hannak E, Oegema K, Kirkham M, Gonczy P, Habermann B, Hyman AA (2002) The kinetically dominant assembly pathway for centrosomal asters in Caenorhabditis elegans is gamma-tubulin dependent. The Journal of cell biology 157(4): 591-602
Heald R, Tournebize R, Blank T, Sandaltzopoulos R, Becker P, Hyman A, Karsenti E (1996) Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382(6590): 420-425
Helps NR, Luo X, Barker HM, Cohen PT (2000) NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase localized to centrosomes, is complexed to protein phosphatase 1. The Biochemical journal 349(Pt 2): 509-518
Herbert B, Pitts AE, Baker SI, Hamilton SE, Wright WE, Shay JW, Corey DR (1999) Inhibition of human telomerase in immortal human cells leads to progressive telomere shortening and cell death. Proceedings of the National Academy of Sciences of the United States of America 96(25): 14276-14281
Hinchcliffe EH, Miller FJ, Cham M, Khodjakov A, Sluder G (2001) Requirement of a centrosomal activity for cell cycle progression through G1 into S phase. Science (New York, NY 291(5508): 1547-1550
Hirota T, Kunitoku N, Sasayama T, Marumoto T, Zhang D, Nitta M, Hatakeyama K, Saya H (2003) Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells. Cell 114(5): 585-598
Holmfeldt P, Stenmark S, Gullberg M (2004) Differential functional interplay of TOGp/XMAP215 and the KinI kinesin MCAK during interphase and mitosis. The EMBO journal 23(3): 627-637
Howard J, Hyman AA (2003) Dynamics and mechanics of the microtubule plus end. Nature 422(6933): 753-758
Huang J, Raff JW (1999) The disappearance of cyclin B at the end of mitosis is regulated spatially in Drosophila cells. The EMBO journal 18(8): 2184-2195
Iarmarcovai G, Botta A, Orsiere T (2006) Number of centromeric signals in micronuclei and mechanisms of aneuploidy. Toxicol Lett 166(1): 1-10
Jackman M, Lindon C, Nigg EA, Pines J (2003) Active cyclin B1-Cdk1 first appears on centrosomes in prophase. Nat Cell Biol 5(2): 143-148
Jang YJ, Ma S, Terada Y, Erikson RL (2002) Phosphorylation of threonine 210 and the role of serine 137 in the regulation of mammalian polo-like kinase. J Biol Chem 277(46): 44115-44120
Job D, Valiron O, Oakley B (2003) Microtubule nucleation. Curr Opin Cell Biol 15(1): 111-117
Karsenti E, Vernos I (2001) The mitotic spindle: a self-made machine. Science (New York, NY 294(5542): 543-547
Katayama H, Brinkley WR, Sen S (2003) The Aurora kinases: role in cell transformation and tumorigenesis. Cancer Metastasis Rev 22(4): 451-464
Katayama H, Sasai K, Kawai H, Yuan ZM, Bondaruk J, Suzuki F, Fujii S, Arlinghaus RB, Czerniak BA, Sen S (2004) Phosphorylation by aurora kinase A induces Mdm2-mediated destabilization and inhibition of p53. Nat Genet 36(1): 55-62
Kemp CA, Kopish KR, Zipperlen P, Ahringer J, O'Connell KF (2004) Centrosome maturation and duplication in C. elegans require the coiled-coil protein SPD-2. Dev Cell 6(4): 511-523
Keryer G, Witczak O, Delouvee A, Kemmner WA, Rouillard D, Tasken K, Bornens M (2003) Dissociating the centrosomal matrix protein AKAP450 from centrioles impairs centriole duplication and cell cycle progression. Mol Biol Cell 14(6): 2436-2446
Khodjakov A, Cole RW, Oakley BR, Rieder CL (2000) Centrosome-independent mitotic spindle formation in vertebrates. Curr Biol 10(2): 59-67
Khodjakov A, Rieder CL (1999) The sudden recruitment of gamma-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules. The Journal of cell biology 146(3): 585-596
Khodjakov A, Rieder CL (2001) Centrosomes enhance the fidelity of cytokinesis in vertebrates and are required for cell cycle progression. The Journal of cell biology 153(1): 237-242
Khodjakov A, Rieder CL, Sluder G, Cassels G, Sibon O, Wang CL (2002) De novo formation of centrosomes in vertebrate cells arrested during S phase. The Journal of cell biology 158(7): 1171-1181
Kim SH, Kaminker P, Campisi J (1999) TIN2, a new regulator of telomere length in human cells. Nat Genet 23(4): 405-412
Kinoshita K, Habermann B, Hyman AA (2002) XMAP215: a key component of the dynamic microtubule cytoskeleton. Trends Cell Biol 12(6): 267-273
Kinoshita K, Noetzel TL, Pelletier L, Mechtler K, Drechsel DN, Schwager A, Lee M, Raff JW,Hyman AA (2005) Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis. The Journal of cell biology 170(7): 1047-1055
Kishi S, Wulf G, Nakamura M, Lu KP (2001) Telomeric protein Pin2/TRF1 induces mitotic entry and apoptosis in cells with short telomeres and is down-regulated in human breast tumors. Oncogene 20(12): 1497-1508
Kiyono T, Foster SA, Koop JI, McDougall JK, Galloway DA, Klingelhutz AJ (1998) Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 396(6706): 84-88
Kline-Smith SL, Walczak CE (2004) Mitotic spindle assembly and chromosome segregation: refocusing on microtubule dynamics. Mol Cell 15(3): 317-327
Klingelhutz AJ, Foster SA, McDougall JK (1996) Telomerase activation by the E6 gene product of human papillomavirus type 16. Nature 380(6569): 79-82
Kramer A, Mailand N, Lukas C, Syljuasen RG, Wilkinson CJ, Nigg EA, Bartek J, Lukas J (2004) Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase. Nat Cell Biol 6(9): 884-891
Kufer TA, Sillje HH, Korner R, Gruss OJ, Meraldi P, Nigg EA (2002) Human TPX2 is required for targeting Aurora-A kinase to the spindle. The Journal of cell biology 158(4): 617-623
Kunitoku N, Sasayama T, Marumoto T, Zhang D, Honda S, Kobayashi O, Hatakeyama K, Ushio Y, Saya H, Hirota T (2003) CENP-A phosphorylation by Aurora-A in prophase is required for enrichment of Aurora-B at inner centromeres and for kinetochore function. Dev Cell 5(6): 853-864
LaBranche H, Dupuis S, Ben-David Y, Bani MR, Wellinger RJ, Chabot B (1998) Telomere elongation by hnRNP A1 and a derivative that interacts with telomeric repeats and telomerase. Nat Genet 19(2): 199-202
Lacey KR, Jackson PK, Stearns T (1999) Cyclin-dependent kinase control of centrosome duplication. Proceedings of the National Academy of Sciences of the United States of America 96(6): 2817-2822
Lan W, Zhang X, Kline-Smith SL, Rosasco SE, Barrett-Wilt GA, Shabanowitz J, Hunt DF, Walczak CE, Stukenberg PT (2004) Aurora B phosphorylates centromeric MCAK and regulates its localization and microtubule depolymerization activity. Curr Biol 14(4): 273-286
Lanni JS, Jacks T (1998) Characterization of the p53-dependent postmitotic checkpoint following spindle disruption. Mol Cell Biol 18(2): 1055-1064
Le Bot N, Tsai MC, Andrews RK, Ahringer J (2003) TAC-1, a regulator of microtubule length inthe C. elegans embryo. Curr Biol 13(17): 1499-1505
Lee HW, Blasco MA, Gottlieb GJ, Horner JW, 2nd, Greider CW, DePinho RA (1998) Essential role of mouse telomerase in highly proliferative organs. Nature 392(6676): 569-574
Lee KS, Grenfell TZ, Yarm FR, Erikson RL (1998) Mutation of the polo-box disrupts localization and mitotic functions of the mammalian polo kinase Plk. Proceedings of the National Academy of Sciences of the United States of America 95(16): 9301-9306
Lee MJ, Gergely F, Jeffers K, Peak-Chew SY, Raff JW (2001) Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour. Nat Cell Biol 3(7): 643-649
Lin J, Blackburn EH (2004) Nucleolar protein PinX1p regulates telomerase by sequestering its protein catalytic subunit in an inactive complex lacking telomerase RNA. Genes Dev 18(4): 387-396
Lingle WL, Lutz WH, Ingle JN, Maihle NJ, Salisbury JL (1998) Centrosome hypertrophy in human breast tumors: implications for genomic stability and cell polarity. Proceedings of the National Academy of Sciences of the United States of America 95(6): 2950-2955
Liu Q, Kaneko S, Yang L, Feldman RI, Nicosia SV, Chen J, Cheng JQ (2004) Aurora-A abrogation of p53 DNA binding and transactivation activity by phosphorylation of serine 215. J Biol Chem 279(50): 52175-52182
Lu KP, Hanes SD, Hunter T (1996) A human peptidyl-prolyl isomerase essential for regulation of mitosis. Nature 380(6574): 544-547
Lustig AJ, Kurtz S, Shore D (1990) Involvement of the silencer and UAS binding protein RAP1 in regulation of telomere length. Science (New York, NY 250(4980): 549-553
Marumoto T, Honda S, Hara T, Nitta M, Hirota T, Kohmura E, Saya H (2003) Aurora-A kinase maintains the fidelity of early and late mitotic events in HeLa cells. J Biol Chem 278(51): 51786-51795
Matsumoto Y, Maller JL (2004) A centrosomal localization signal in cyclin E required for Cdk2-independent S phase entry. Science (New York, NY 306(5697): 885-888
Mayor T, Hacker U, Stierhof YD, Nigg EA (2002) The mechanism regulating the dissociation of the centrosomal protein C-Nap1 from mitotic spindle poles. J Cell Sci 115(Pt 16): 3275-3284
McEachern MJ, Krauskopf A, Blackburn EH (2000) Telomeres and their control. Annu Rev Genet 34: 331-358
Megraw TL, Kao LR, Kaufman TC (2001) Zygotic development without functional mitotic centrosomes. Curr Biol 11(2): 116-120
Mendez R, Richter JD (2001) Translational control by CPEB: a means to the end. Nature reviews 2(7): 521-529
Meraldi P, Honda R, Nigg EA (2002) Aurora-A overexpression reveals tetraploidization as a major route to centrosome amplification in p53-/- cells. The EMBO journal 21(4): 483-492
Meraldi P, Lukas J, Fry AM, Bartek J, Nigg EA (1999) Centrosome duplication in mammalian somatic cells requires E2F and Cdk2-cyclin A. Nat Cell Biol 1(2): 88-93
Moore A, Wordeman L (2004) The mechanism, function and regulation of depolymerizing kinesins during mitosis. Trends Cell Biol 14(10): 537-546
Nakamura M, Zhou XZ, Kishi S, Kosugi I, Tsutsui Y, Lu KP (2001) A specific interaction between the telomeric protein Pin2/TRF1 and the mitotic spindle. Curr Biol 11(19): 1512-1516
Nigg EA (2001) Mitotic kinases as regulators of cell division and its checkpoints. Nature reviews 2(1): 21-32
Nigg EA (2002) Centrosome aberrations: cause or consequence of cancer progression? Nature reviews 2(11): 815-825
Noetzel TL, Drechsel DN, Hyman AA, Kinoshita K (2005) A comparison of the ability of XMAP215 and tau to inhibit the microtubule destabilizing activity of XKCM1. Philosophical transactions of the Royal Society of London 360(1455): 591-594
Oh BK, Yoon SM, Lee CH, Park YN (2007) Rat homolog of PinX1 is a nucleolar protein involved in the regulation of telomere length. Gene 400(1-2): 35-43
Ohkura H, Garcia MA, Toda T (2001) Dis1/TOG universal microtubule adaptors - one MAP for all? J Cell Sci 114(Pt 21): 3805-3812
Okuda M, Horn HF, Tarapore P, Tokuyama Y, Smulian AG, Chan PK, Knudsen ES, Hofmann IA, Snyder JD, Bove KE, Fukasawa K (2000) Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication. Cell 103(1): 127-140
Pascreau G, Delcros JG, Cremet JY, Prigent C, Arlot-Bonnemains Y (2005) Phosphorylation of maskin by Aurora-A participates in the control of sequential protein synthesis during Xenopus laevis oocyte maturation. J Biol Chem 280(14): 13415-13423
Piehl M, Tulu US, Wadsworth P, Cassimeris L (2004) Centrosome maturation: measurement of microtubule nucleation throughout the cell cycle by using GFP-tagged EB1. Proceedings of the National Academy of Sciences of the United States of America 101(6): 1584-1588
Piel M, Nordberg J, Euteneuer U, Bornens M (2001) Centrosome-dependent exit of cytokinesis in animal cells. Science (New York, NY 291(5508): 1550-1553
Pihan GA, Purohit A, Wallace J, Knecht H, Woda B, Quesenberry P, Doxsey SJ (1998)
Centrosome defects and genetic instability in malignant tumors. Cancer Res 58(17): 3974-3985
Popov AV, Pozniakovsky A, Arnal I, Antony C, Ashford AJ, Kinoshita K, Tournebize R, Hyman AA, Karsenti E (2001) XMAP215 regulates microtubule dynamics through two distinctdomains. The EMBO journal 20(3): 397-410
Popov AV, Severin F, Karsenti E (2002) XMAP215 is required for the microtubule-nucleating activity of centrosomes. Curr Biol 12(15): 1326-1330
Prigozhina NL, Oakley CE, Lewis AM, Nayak T, Osmani SA, Oakley BR (2004) gamma-tubulin plays an essential role in the coordination of mitotic events. Mol Biol Cell 15(3): 1374-1386 Raff JW (2001) Centrosomes: Central no more? Curr Biol 11(5): R159-161
Raff JW (2002) Centrosomes and cancer: lessons from a TACC. Trends Cell Biol 12(5): 222-225
Raff JW, Jeffers K, Huang JY (2002) The roles of Fzy/Cdc20 and Fzr/Cdh1 in regulating the destruction of cyclin B in space and time. The Journal of cell biology 157(7): 1139-1149
Rajagopalan S, Bimbo A, Balasubramanian MK, Oliferenko S (2004) A potential tension-sensing mechanism that ensures timely anaphase onset upon metaphase spindle orientation. Curr Biol 14(1): 69-74
Reynolds N, Ohkura H (2003) Polo boxes form a single functional domain that mediates interactions with multiple proteins in fission yeast polo kinase. J Cell Sci 116(Pt 7): 1377-1387
Roghi C, Giet R, Uzbekov R, Morin N, Chartrain I, Le Guellec R, Couturier A, Doree M, Philippe M, Prigent C (1998) The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly. J Cell Sci 111 ( Pt 5): 557-572
Sampath SC, Ohi R, Leismann O, Salic A, Pozniakovski A, Funabiki H (2004) The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly. Cell 118(2): 187-202
Sankaran S, Parvin JD (2006) Centrosome function in normal and tumor cells. J Cell Biochem 99(5): 1240-1250
Shay JW, Wright WE (2001) Telomeres and telomerase: implications for cancer and aging. Radiat Res 155(1 Pt 2): 188-193
Shen M, Haggblom C, Vogt M, Hunter T, Lu KP (1997) Characterization and cell cycle regulation of the related human telomeric proteins Pin2 and TRF1 suggest a role in mitosis. Proceedings of the National Academy of Sciences of the United States of America 94(25): 13618-13623
Sherr CJ (1996) Cancer cell cycles. Science (New York, NY 274(5293): 1672-1677
Shimamura M, Brown RC, Lemmon BE, Akashi T, Mizuno K, Nishihara N, Tomizawa K, Yoshimoto K, Deguchi H, Hosoya H, Horio T, Mineyuki Y (2004) Gamma-tubulin in basal land plants: characterization, localization, and implication in the evolution of acentriolar microtubule organizing centers. Plant Cell 16(1): 45-59
Smith S, Giriat I, Schmitt A, de Lange T (1998) Tankyrase, a poly(ADP-ribose) polymerase athuman telomeres. Science (New York, NY 282(5393): 1484-1487
Smogorzewska A, van Steensel B, Bianchi A, Oelmann S, Schaefer MR, Schnapp G, de Lange T (2000) Control of human telomere length by TRF1 and TRF2. Mol Cell Biol 20(5): 1659-1668
Srayko M, Quintin S, Schwager A, Hyman AA (2003) Caenorhabditis elegans TAC-1 and ZYG-9 form a complex that is essential for long astral and spindle microtubules. Curr Biol 13(17): 1506-1511
Srsen V, Merdes A (2006) The centrosome and cell proliferation. Cell division 1: 26
Stebbins-Boaz B, Cao Q, de Moor CH, Mendez R, Richter JD (1999) Maskin is a CPEB-associated factor that transiently interacts with elF-4E. Mol Cell 4(6): 1017-1027
Stewart SA, Weinberg RA (2000) Telomerase and human tumorigenesis. Semin Cancer Biol 10(6): 399-406
Still IH, Vettaikkorumakankauv AK, DiMatteo A, Liang P (2004) Structure-function evolution of the transforming acidic coiled coil genes revealed by analysis of phylogenetically diverse organisms. BMC Evol Biol 4: 16
Taguchi S, Honda K, Sugiura K, Yamaguchi A, Furukawa K, Urano T (2002) Degradation of human Aurora-A protein kinase is mediated by hCdh1. FEBS Lett 519(1-3): 59-65
Takai N, Hamanaka R, Yoshimatsu J, Miyakawa I (2005) Polo-like kinases (Plks) and cancer. Oncogene 24(2): 287-291
Tatsuka M, Katayama H, Ota T, Tanaka T, Odashima S, Suzuki F, Terada Y (1998) Multinuclearity and increased ploidy caused by overexpression of the aurora- and Ipl1-like midbody-associated protein mitotic kinase in human cancer cells. Cancer Res 58(21): 4811-4816
Tavares AA, Glover DM, Sunkel CE (1996) The conserved mitotic kinase polo is regulated by phosphorylation and has preferred microtubule-associated substrates in Drosophila embryo extracts. The EMBO journal 15(18): 4873-4883
Terada Y, Uetake Y, Kuriyama R (2003) Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells. The Journal of cell biology 162(5): 757-763
Theurkauf WE, Hawley RS (1992) Meiotic spindle assembly in Drosophila females: behavior of nonexchange chromosomes and the effects of mutations in the nod kinesin-like protein. The Journal of cell biology 116(5): 1167-1180
Tokuyama Y, Horn HF, Kawamura K, Tarapore P, Fukasawa K (2001) Specific phosphorylation of nucleophosmin on Thr(199) by cyclin-dependent kinase 2-cyclin E and its role in centrosome duplication. J Biol Chem 276(24): 21529-21537
Tournebize R, Popov A, Kinoshita K, Ashford AJ, Rybina S, Pozniakovsky A, Mayer TU, WalczakCE, Karsenti E, Hyman AA (2000) Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts. Nat Cell Biol 2(1): 13-19
Trielli MO, Andreassen PR, Lacroix FB, Margolis RL (1996) Differential Taxol-dependent arrest of transformed and nontransformed cells in the G1 phase of the cell cycle, and specific-related mortality of transformed cells. The Journal of cell biology 135(3): 689-700
Tsai MY, Wiese C, Cao K, Martin O, Donovan P, Ruderman J, Prigent C, Zheng Y (2003) A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly. Nat Cell Biol 5(3): 242-248
Tsou MF, Stearns T (2006) Controlling centrosome number: licenses and blocks. Curr Opin Cell Biol 18(1): 74-78
Uetake Y, Sluder G (2004) Cell cycle progression after cleavage failure: mammalian somatic cells do not possess a "tetraploidy checkpoint". The Journal of cell biology 165(5): 609-615
Usui T, Maekawa H, Pereira G, Schiebel E (2003) The XMAP215 homologue Stu2 at yeast spindle pole bodies regulates microtubule dynamics and anchorage. The EMBO journal 22(18): 4779-4793
van Overbeek M, de Lange T (2006) Apollo, an Artemis-related nuclease, interacts with TRF2 and protects human telomeres in S phase. Curr Biol 16(13): 1295-1302
van Steensel B, de Lange T (1997) Control of telomere length by the human telomeric protein TRF1. Nature 385(6618): 740-743
Vandre DD, Borisy GG (1989) Anaphase onset and dephosphorylation of mitotic phosphoproteins occur concomitantly. J Cell Sci 94 ( Pt 2): 245-258
Vaziri H, Benchimol S (1998) Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span. Curr Biol 8(5): 279-282
Verde F, Dogterom M, Stelzer E, Karsenti E, Leibler S (1992) Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts. The Journal of cell biology 118(5): 1097-1108
Wakefield JG, Huang JY, Raff JW (2000) Centrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos. Curr Biol 10(21): 1367-1370
Walczak CE, Mitchison TJ, Desai A (1996) XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly. Cell 84(1): 37-47
Walker RA, O'Brien ET, Pryer NK, Soboeiro MF, Voter WA, Erickson HP, Salmon ED (1988) Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies. The Journal of cell biology 107(4): 1437-1448
Wang J, Xie LY, Allan S, Beach D, Hannon GJ (1998) Myc activates telomerase. Genes Dev12(12): 1769-1774
Warnke S, Kemmler S, Hames RS, Tsai HL, Hoffmann-Rohrer U, Fry AM, Hoffmann I (2004) Polo-like kinase-2 is required for centriole duplication in mammalian cells. Curr Biol 14(13): 1200-1207
Wittmann T, Hyman A, Desai A (2001) The spindle: a dynamic assembly of microtubules and motors. Nat Cell Biol 3(1): E28-34
Wong C, Stearns T (2005) Mammalian cells lack checkpoints for tetraploidy, aberrant centrosome number, and cytokinesis failure. BMC Cell Biol 6(1): 6
Wu KJ, Grandori C, Amacker M, Simon-Vermot N, Polack A, Lingner J, Dalla-Favera R (1999) Direct activation of TERT transcription by c-MYC. Nat Genet 21(2): 220-224
Yoo JE, Oh BK, Park YN (2009) Human PinX1 Mediates TRF1 Accumulation in Nucleolus and Enhances TRF1 Binding to Telomeres. J Mol Biol
Young AC, Chavez M, Giambernardi TA, Mattern V, McGill JR, Harris JM, Sarosdy MF, Patel P, Sakaguchi AY (1997) Organization and expression of human telomere repeat binding factor genes. Somat Cell Mol Genet 23(4): 275-286
Zhang X, Mar V, Zhou W, Harrington L, Robinson MO (1999) Telomere shortening and apoptosis in telomerase-inhibited human tumor cells. Genes Dev 13(18): 2388-2399
Zhou J, Monson EK, Teng SC, Schulz VP, Zakian VA (2000) Pif1p helicase, a catalytic inhibitor of telomerase in yeast. Science (New York, NY 289(5480): 771-774
Zhou XZ, Lu KP (2001) The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor. Cell 107(3): 347-359
Zhu XD, Niedernhofer L, Kuster B, Mann M, Hoeijmakers JH, de Lange T (2003) ERCC1/XPF removes the 3' overhang from uncapped telomeres and represses formation of telomeric DNA-containing double minute chromosomes. Mol Cell 12(6): 1489-1498
Zimmerman WC, Sillibourne J, Rosa J, Doxsey SJ (2004) Mitosis-specific anchoring of gamma tubulin complexes by pericentrin controls spindle organization and mitotic entry. Mol Biol Cell 15(8): 3642-3657
Barros TP, Kinoshita K, Hyman AA, Raff JW (2005) Aurora A activates D-TACC-Msps complexes exclusively at centrosomes to stabilize centrosomal microtubules. The Journal of cell biology 170(7): 1039-1046
Chen HM, Schmeichel KL, Mian IS, Lelievre S, Petersen OW, Bissell MJ (2000) AZU-1: a candidate breast tumor suppressor and biomarker for tumor progression. Mol Biol Cell 11(4): 1357-1367
D'Assoro AB, Lingle WL, Salisbury JL (2002) Centrosome amplification and the development of cancer. Oncogene 21(40): 6146-6153
Doxsey S, Zimmerman W, Mikule K (2005) Centrosome control of the cell cycle. Trends Cell Biol 15(6): 303-311
Gergely F (2002) Centrosomal TACCtics. Bioessays 24(10): 915-925
Gergely F, Karlsson C, Still I, Cowell J, Kilmartin J, Raff JW (2000) The TACC domain identifies a family of centrosomal proteins that can interact with microtubules. Proceedings of the National Academy of Sciences of the United States of America 97(26): 14352-14357
Giet R, McLean D, Descamps S, Lee MJ, Raff JW, Prigent C, Glover DM (2002) Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules. The Journal of cell biology 156(3): 437-451
Harrington EA, Bebbington D, Moore J, Rasmussen RK, Ajose-Adeogun AO, Nakayama T, Graham JA, Demur C, Hercend T, Diu-Hercend A, Su M, Golec JM, Miller KM (2004) VX-680, a potent and selective small-molecule inhibitor of the Aurora kinases, suppresses tumor growth in vivo. Nat Med 10(3): 262-267
Iarmarcovai G, Botta A, Orsiere T (2006) Number of centromeric signals in micronuclei and mechanisms of aneuploidy. Toxicol Lett 166(1): 1-10
Kinoshita K, Noetzel TL, Pelletier L, Mechtler K, Drechsel DN, Schwager A, Lee M, Raff JW, Hyman AA (2005) Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis. The Journal of cell biology 170(7): 1047-1055
Lauffart B, Gangisetty O, Still IH (2003) Molecular cloning, genomic structure and interactions of the putative breast tumor suppressor TACC2. Genomics 81(2): 192-201
LeRoy PJ, Hunter JJ, Hoar KM, Burke KE, Shinde V, Ruan J, Bowman D, Galvin K, Ecsedy JA (2007) Localization of human TACC3 to mitotic spindles is mediated by phosphorylation on Ser558 by Aurora A: a novel pharmacodynamic method for measuring Aurora A activity.Cancer Res 67(11): 5362-5370
Pascreau G, Delcros JG, Cremet JY, Prigent C, Arlot-Bonnemains Y (2005) Phosphorylation of maskin by Aurora-A participates in the control of sequential protein synthesis during Xenopus laevis oocyte maturation. J Biol Chem 280(14): 13415-13423
Peset I, Seiler J, Sardon T, Bejarano LA, Rybina S, Vernos I (2005) Function and regulation of Maskin, a TACC family protein, in microtubule growth during mitosis. The Journal of cell biology 170(7): 1057-1066
Raff JW (2002) Centrosomes and cancer: lessons from a TACC. Trends Cell Biol 12(5): 222-225
Schuendeln MM, Piekorz RP, Wichmann C, Lee Y, McKinnon PJ, Boyd K, Takahashi Y, Ihle JN (2004) The centrosomal, putative tumor suppressor protein TACC2 is dispensable for normal development, and deficiency does not lead to cancer. Mol Cell Biol 24(14): 6403-6409
Srsen V, Merdes A (2006) The centrosome and cell proliferation. Cell division 1: 26 Still IH, Vettaikkorumakankauv AK, DiMatteo A, Liang P (2004) Structure-function evolution of the transforming acidic coiled coil genes revealed by analysis of phylogenetically diverse organisms. BMC Evol Biol 4: 16
Tien AC, Lin MH, Su LJ, Hong YR, Cheng TS, Lee YC, Lin WJ, Still IH, Huang CY (2004) Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model. Mol Cell Proteomics 3(1): 93-104
Banik SS, Counter CM (2004) Characterization of interactions between PinX1 and human telomerase subunits hTERT and hTR. J Biol Chem 279(50): 51745-51748
Blower MD, Nachury M, Heald R, Weis K (2005) A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. Cell 121(2): 223-234
Chan GK, Jablonski SA, Starr DA, Goldberg ML, Yen TJ (2000) Human Zw10 and ROD are mitotic checkpoint proteins that bind to kinetochores. Nat Cell Biol 2(12): 944-947
Cheeseman IM, Chappie JS, Wilson-Kubalek EM, Desai A (2006) The conserved KMN network constitutes the core microtubule-binding site of the kinetochore. Cell 127(5): 983-997
DeLuca JG, Gall WE, Ciferri C, Cimini D, Musacchio A, Salmon ED (2006) Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. Cell 127(5): 969-982
Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR, 3rd, Cleveland DW (2006) The human CENP-A centromeric nucleosome-associated complex. Nat Cell Biol 8(5): 458-469
Fujita Y, Hayashi T, Kiyomitsu T, Toyoda Y, Kokubu A, Obuse C, Yanagida M (2007) Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1. Dev Cell 12(1): 17-30
Guglielmi B, Werner M (2002) The yeast homolog of human PinX1 is involved in rRNA and small nucleolar RNA maturation, not in telomere elongation inhibition. J Biol Chem 277(38): 35712-35719
Heald R, Tournebize R, Blank T, Sandaltzopoulos R, Becker P, Hyman A, Karsenti E (1996) Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382(6590): 420-425
Kalab P, Weis K, Heald R (2002) Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts. Science (New York, NY 295(5564): 2452-2456
Lin J, Blackburn EH (2004) Nucleolar protein PinX1p regulates telomerase by sequestering its protein catalytic subunit in an inactive complex lacking telomerase RNA. Genes Dev 18(4): 387-396
Lin J, Jin R, Zhang B, Yang PX, Chen H, Bai YX, Xie Y, Huang C, Huang J (2007) Characterization of a novel effect of hPinX1 on hTERT nucleolar localization. Biochem Biophys Res Commun 353(4): 946-952
Liu D, Ding X, Du J, Cai X, Huang Y, Ward T, Shaw A, Yang Y, Hu R, Jin C, Yao X (2007) Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment. J Biol Chem 282(29): 21415-21424
Lou Y, Yao J, Zereshki A, Dou Z, Ahmed K, Wang H, Hu J, Wang Y, Yao X (2004) NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling. J Biol Chem 279(19): 20049-20057
Ma N, Matsunaga S, Takata H, Ono-Maniwa R, Uchiyama S, Fukui K (2007) Nucleolin functions in nucleolus formation and chromosome congression. J Cell Sci 120(Pt 12): 2091-2105
Mao Y, Desai A, Cleveland DW (2005) Microtubule capture by CENP-E silences BubR1-dependent mitotic checkpoint signaling. The Journal of cell biology 170(6): 873-880
Mazumdar M, Misteli T (2005) Chromokinesins: multitalented players in mitosis. Trends Cell Biol 15(7): 349-355
McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ, Stukenberg PT (2003) The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev 17(1): 101-114
McCleland ML, Kallio MJ, Barrett-Wilt GA, Kestner CA, Shabanowitz J, Hunt DF, Gorbsky GJ, Stukenberg PT (2004) The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are required to establish and maintain kinetochore-microtubule attachment. Curr Biol 14(2): 131-137
Musacchio A, Salmon ED (2007) The spindle-assembly checkpoint in space and time. Nature reviews 8(5): 379-393
Nachury MV, Maresca TJ, Salmon WC, Waterman-Storer CM, Heald R, Weis K (2001) Importin beta is a mitotic target of the small GTPase Ran in spindle assembly. Cell 104(1): 95-106 Nakamura M, Zhou XZ, Kishi S, Kosugi I, Tsutsui Y, Lu KP (2001) A specific interaction between the telomeric protein Pin2/TRF1 and the mitotic spindle. Curr Biol 11(19): 1512-1516
Oh BK, Yoon SM, Lee CH, Park YN (2007) Rat homolog of PinX1 is a nucleolar protein involved in the regulation of telomere length. Gene 400(1-2): 35-43
Rieder CL (1982) The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber. Int Rev Cytol 79: 1-58
Sakita-Suto S, Kanda A, Suzuki F, Sato S, Takata T, Tatsuka M (2007) Aurora-B Regulates RNA Methyltransferase NSUN2. Mol Biol Cell
Tahara K, Takagi M, Ohsugi M, Sone T, Nishiumi F, Maeshima K, Horiuchi Y, Tokai-Nishizumi N, Imamoto F, Yamamoto T, Kose S, Imamoto N (2008) Importin-beta and the small guanosine triphosphatase Ran mediate chromosome loading of the human chromokinesin Kid. The Journal of cell biology 180(3): 493-506
Uchiyama S, Kobayashi S, Takata H, Ishihara T, Hori N, Higashi T, Hayashihara K, Sone T, Higo D, Nirasawa T, Takao T, Matsunaga S, Fukui K (2005) Proteome analysis of human metaphasechromosomes. J Biol Chem 280(17): 16994-17004
Van Hooser AA, Yuh P, Heald R (2005) The perichromosomal layer. Chromosoma 114(6): 377-388
Vergnolle MA, Taylor SS (2007) Cenp-F Links Kinetochores to Ndel1/Nde1/Lis1/Dynein Microtubule Motor Complexes. Curr Biol
Wang H, Hu X, Ding X, Dou Z, Yang Z, Shaw AW, Teng M, Cleveland DW, Goldberg ML, Niu L, Yao X (2004) Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling. J Biol Chem 279(52): 54590-54598
Wei RR, Al-Bassam J, Harrison SC (2007) The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment. Nat Struct Mol Biol 14(1): 54-59
Wigge PA, Kilmartin JV (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. The Journal of cell biology 152(2): 349-360
Wright K, Wilson PJ, Kerr J, Do K, Hurst T, Khoo SK, Ward B, Chenevix-Trench G (1998) Frequent loss of heterozygosity and three critical regions on the short arm of chromosome 8 in ovarian adenocarcinomas. Oncogene 17(9): 1185-1188
Yang Y, Wu F, Ward T, Yan F, Wu Q, Wang Z, McGlothen T, Peng W, You T, Cui T, Hu R, Dou Z, Zhu J, Xie W, Rao Z, Yao X (2008) Phosphorylation of HsMis13 by aurora B is essential for assembly of functional kinetochore. J Biol Chem
Yao X, Abrieu A, Zheng Y, Sullivan KF, Cleveland DW (2000) CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint. Nat Cell Biol 2(8): 484-491
Yao X, Anderson KL, Cleveland DW (1997) The microtubule-dependent motor centromere-associated protein E (CENP-E) is an integral component of kinetochore corona fibers that link centromeres to spindle microtubules. The Journal of cell biology 139(2): 435-447
Zhou XZ, Lu KP (2001) The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor. Cell 107(3): 347-359
Banik SS, Counter CM (2004) Characterization of interactions between PinX1 and human telomerase subunits hTERT and hTR. J Biol Chem 279(50): 51745-51748
Bertuch AA, Lundblad V (2003) The Ku heterodimer performs separable activities at double-strand breaks and chromosome termini. Mol Cell Biol 23(22): 8202-8215
Cao X, Ding X, Guo Z, Zhou R, Wang F, Long F, Wu F, Bi F, Wang Q, Fan D, Forte JG, Teng M, Yao X (2005) PALS1 specifies the localization of ezrin to the apical membrane of gastric parietal cells. J Biol Chem 280(14): 13584-13592
Dundr M, Meier UT, Lewis N, Rekosh D, Hammarskjold ML, Olson MO (1997) A class of nonribosomal nucleolar components is located in chromosome periphery and in nucleolus-derived foci during anaphase and telophase. Chromosoma 105(7-8): 407-417
Earnshaw WC, Halligan B, Cooke CA, Heck MM, Liu LF (1985) Topoisomerase II is a structural component of mitotic chromosome scaffolds. The Journal of cell biology 100(5): 1706-1715
Gautier T, Masson C, Quintana C, Arnoult J, Hernandez-Verdun D (1992) The ultrastructure of the chromosome periphery in human cell lines. An in situ study using cryomethods in electron microscopy. Chromosoma 101(8): 502-510
Hanakahi LA, Sun H, Maizels N (1999) High affinity interactions of nucleolin with G-G-paired rDNA. J Biol Chem 274(22): 15908-15912
Heald R, Tournebize R, Blank T, Sandaltzopoulos R, Becker P, Hyman A, Karsenti E (1996) Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382(6590): 420-425
Hernandez-Verdun D, Gautier T (1994) The chromosome periphery during mitosis. Bioessays 16(3): 179-185
Hood FE, Clarke PR (2007) RCC1 isoforms differ in their affinity for chromatin, molecular interactions and regulation by phosphorylation. J Cell Sci 120(Pt 19): 3436-3445
Lee YM, Lee S, Lee E, Shin H, Hahn H, Choi W, Kim W (2001) Human kinesin superfamily member 4 is dominantly localized in the nuclear matrix and is associated with chromosomes during mitosis. The Biochemical journal 360(Pt 3): 549-556
Lin J, Blackburn EH (2004) Nucleolar protein PinX1p regulates telomerase by sequestering its protein catalytic subunit in an inactive complex lacking telomerase RNA. Genes Dev 18(4): 387-396
Lin J, Jin R, Zhang B, Yang PX, Chen H, Bai YX, Xie Y, Huang C, Huang J (2007) Characterization of a novel effect of hPinX1 on hTERT nucleolar localization. BiochemBiophys Res Commun 353(4): 946-952
Ma N, Matsunaga S, Takata H, Ono-Maniwa R, Uchiyama S, Fukui K (2007) Nucleolin functions in nucleolus formation and chromosome congression. J Cell Sci 120(Pt 12): 2091-2105
McGill M, Brinkley BR (1975) Human chromosomes and centrioles as nucleating sites for the in vitro assembly of microtubules from bovine brain tubulin. The Journal of cell biology 67(1): 189-199
Mitchison TJ, Kirschner MW (1985) Properties of the kinetochore in vitro. I. Microtubule nucleation and tubulin binding. The Journal of cell biology 101(3): 755-765
Sharp LW (1929) Structure of large somatic chromosomes. Bot Gaz 88: 349-382
Srivastava M, Pollard HB (1999) Molecular dissection of nucleolin's role in growth and cell proliferation: new insights. Faseb J 13(14): 1911-1922
Takagi M, Matsuoka Y, Kurihara T, Yoneda Y (1999) Chmadrin: a novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure. J Cell Sci 112 ( Pt 15): 2463-2472
Takata H, Matsunaga S, Morimoto A, Ono-Maniwa R, Uchiyama S, Fukui K (2007) H1.X with different properties from other linker histones is required for mitotic progression. FEBS Lett 581(20): 3783-3788
Van Hooser AA, Yuh P, Heald R (2005) The perichromosomal layer. Chromosoma 114(6): 377-388
Wollman R, Cytrynbaum EN, Jones JT, Meyer T, Scholey JM, Mogilner A (2005) Efficient chromosome capture requires a bias in the 'search-and-capture' process during mitotic-spindle assembly. Curr Biol 15(9): 828-832
Yajima J, Edamatsu M, Watai-Nishii J, Tokai-Nishizumi N, Yamamoto T, Toyoshima YY (2003) The human chromokinesin Kid is a plus end-directed microtubule-based motor. The EMBO journal 22(5): 1067-1074
Yuan K, Hu H, Guo Z, Fu G, Shaw AP, Hu R, Yao X (2007) Phospho-regulation of HsCdc14A By Polo-like kinase 1 is essential for mitotic progression. J Biol Chem 282(37): 27414-27423
Zhou XZ, Lu KP (2001) The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor. Cell 107(3): 347-359