营养补剂对大鼠不同时相糖原储备及股直肌部分有氧酶活性的影响
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摘要
目的：观察营养补剂对大鼠三周递增负荷训练后体内糖原储备及股直肌中部分有氧代谢酶活性的变化。对比分析不同组别相关指标的差异,探索该营养补剂对大鼠糖原储备不同时相的影响,以及对股直肌中部分有氧氧化酶活性的影响。
方法：将90只SD雄性大鼠随机分为安静组(C)、运动组(S)和运动加营养组(T),且S、T组依据运动后0h、1h、6h和24h的宰杀时相,再各自随机平均分为4小组。S组和T组进行三周跑台训练,每周训练六天,每天训练一次。第一周训练时间为40min,第二周50min,第三周60min。第一天训练速度为20m／min,随后隔日递增一次,最终增至38m/min。S、T组每日训练前、后15min分别进行一次灌胃,T组服用营养补剂,S、C组服用等量的水。三周后处死,取材测试。
结果：(1)主效应时相、补剂对不同组间大鼠肝、肌糖原、血糖、INS和股直肌中SDH、MDH的影响差异均具有显著性。(2)S组和T组运动后6h和24h肝、肌糖原含量分别与组内运动后前一时相差异均具有显著性。S组和T组运动后1h血糖与运动后0h相比差异具有显著性。S组和T组运动后24hINS水平与运动后Oh、1h和6h差异均都具有显著性。S组和T组运动后6h SDH和MDH活性与运动后0h差异具有显著性,S组和T组运动后24h SDH和MDH活性与运动后6h差异具有显著性。(3)运动后6h和24h,T组肌糖原与S组差异具有显著性；运动后0h和6h,T组肝糖原与S组差异具有显著性；运动后1h和6h,T组血糖与S组差异具有显著性；运动后1h和24h,T组INS与S组差异具有显著性；运动后0h,T组SDH和MDH与S组差异具有显著性。
结论：(1)营养补剂可明显促进运动后INS的分泌,维持血糖浓度的恒定,提高运动后肝、肌糖原恢复合成的能力。(2)营养补剂可显著提高T组运动后肝、肌糖原合成及血糖浓度,其中,运动后6h肝、肌糖原合成及血糖浓度恢复能力显著好于S组。(3)营养补剂可明显降低运动后0h胞浆中SDH的活性,并可明显增加运动后OhMDH的活性,从而提高能量供应水平。
Objective:The study observes the effects of nutrition supplement on energy material storage and some rectus femoris aerobic metabolism enzyme level in rats after three weeks incremental exercise. The goal is to probe how the nutrition to promote the different groups with the related data.
Method:90SD male rats were random organized into control group (C), exercise group (S), and exercise and nutrition group (T). Then, the group S and T were random organized into 4 groups each other in average, according to the kill time of rats, which ordered after exercise 0 hour, after exercise 1 hour, after exercise 6 hours and after exercise 24 hours. They have no differences of weight among all the groups. Among them, group S and T were taken treadmill exercise for 3 weeks. They trained 6 days per week which had a rest on Sunday, and one time every day in the morning. They trained 40 min at the first week, and then the time was added to 50 min at the second week,60 min at the last week. The speed of running on the first day was 20 m/min, then, it was increased every two days, and reached 38 m/min in the end. Group T was taken nutrition supplement 15 min before the exercise and 15min after. Group S was the same all, but the supplement was water. Besides, group C was taken the equal water as group S. They were all put to death with 2% sedative pentobarbital sodium after 3 weeks, took the blood, liver and rectus femoris of rats to test liver glycogen, muscle glycogen, glucose, INS, SDH and MDH, and used SPSS13.0 to dispose statistics.
Results:(1)Univariated general linear model analysis showed that the main effect nutrition supplement on the liver and muscle Glycogen storage and the level of blood Glucose, serum Insulin, rectus femoris Succinate Dehydrogenas and Malate Dehydrogenase was significant difference between the two groups (P<0.01). (2) One-Way ANOVA showed that the Muscle Glycogen at 6 and 24 hour after exercise was significantly higher than it at the next time within group S and T (P<0.01). The Liver Glycogen at 6 and 24 hour after exercise was significantly higher than it at the next time within group S and T (P<0.01). The Blood Glucose at 1 hour after exercise was significantly higher than it after exercise instantly of group S and T (P<0.01), the Blood Glucose at 6 and 24 hour after exercise was significantly higher than it at 1 hour after exercise of group S and T. The Insulin at 24 hour after exercise was significantly higher than it after exercise instantly and at 1 hour and 6 hour after exercise of group S and T (P<0.01). The rectus femoris Succinate Dehydrogenas at 6 hour after exercise was significantly higher than it after exercise instantly of group S and T. The rectus femoris Malate Dehydrogenase at 6 hour after exercise was significantly higher than it after exercise instantly of group T (P<0.05). (3) T test indicated that the level of Liver Glycogen of group T was significantly higher than that of group S at 6 and 24 hour after exercise (P<0.05). The level of Liver Glycogen of group T was significantly higher than that of group S, after exercise instantly and at 6 hour after exercise (P<0.05). The level of Blood Glucose of group T was significantly higher than that of group S at 1 and 6 hour after exercise (P<0.05). The level of Insulin of group T was significantly higher than that of group S at 1 and 24 hour after exercise (P<0.05). The level of rectus femoris Succinate Dehydrogenas and Malate Dehydrogenase of group T was significantly higher than that of group S after exercise instantly (P<0.05).
Conclusion:(1) Taking nutrition supplements of the rats can significantly enhance the capacity of the liver and muscle glycogen recovery, can improve the serum level of insulin secretion after exercise, and can slower decline the level of blood glucose to maintain it density. (2) Taking nutrition supplements of the rats can significantly enhance the capacity of the liver and muscle glycogen and blood glucose recovery at 6 hour after exercise. (3) Taking nutrition supplements of the rats can significantly disease the level of rectus femoris Succinate Dehydrogenas in cytoplasm and increase the level of rectus femoris Malate Dehydrogenase to enhance the energy supply after exercise instantly.
方法：将90只SD雄性大鼠随机分为安静组(C)、运动组(S)和运动加营养组(T),且S、T组依据运动后0h、1h、6h和24h的宰杀时相,再各自随机平均分为4小组。S组和T组进行三周跑台训练,每周训练六天,每天训练一次。第一周训练时间为40min,第二周50min,第三周60min。第一天训练速度为20m／min,随后隔日递增一次,最终增至38m/min。S、T组每日训练前、后15min分别进行一次灌胃,T组服用营养补剂,S、C组服用等量的水。三周后处死,取材测试。
结果：(1)主效应时相、补剂对不同组间大鼠肝、肌糖原、血糖、INS和股直肌中SDH、MDH的影响差异均具有显著性。(2)S组和T组运动后6h和24h肝、肌糖原含量分别与组内运动后前一时相差异均具有显著性。S组和T组运动后1h血糖与运动后0h相比差异具有显著性。S组和T组运动后24hINS水平与运动后Oh、1h和6h差异均都具有显著性。S组和T组运动后6h SDH和MDH活性与运动后0h差异具有显著性,S组和T组运动后24h SDH和MDH活性与运动后6h差异具有显著性。(3)运动后6h和24h,T组肌糖原与S组差异具有显著性；运动后0h和6h,T组肝糖原与S组差异具有显著性；运动后1h和6h,T组血糖与S组差异具有显著性；运动后1h和24h,T组INS与S组差异具有显著性；运动后0h,T组SDH和MDH与S组差异具有显著性。
结论：(1)营养补剂可明显促进运动后INS的分泌,维持血糖浓度的恒定,提高运动后肝、肌糖原恢复合成的能力。(2)营养补剂可显著提高T组运动后肝、肌糖原合成及血糖浓度,其中,运动后6h肝、肌糖原合成及血糖浓度恢复能力显著好于S组。(3)营养补剂可明显降低运动后0h胞浆中SDH的活性,并可明显增加运动后OhMDH的活性,从而提高能量供应水平。
Objective:The study observes the effects of nutrition supplement on energy material storage and some rectus femoris aerobic metabolism enzyme level in rats after three weeks incremental exercise. The goal is to probe how the nutrition to promote the different groups with the related data.
Method:90SD male rats were random organized into control group (C), exercise group (S), and exercise and nutrition group (T). Then, the group S and T were random organized into 4 groups each other in average, according to the kill time of rats, which ordered after exercise 0 hour, after exercise 1 hour, after exercise 6 hours and after exercise 24 hours. They have no differences of weight among all the groups. Among them, group S and T were taken treadmill exercise for 3 weeks. They trained 6 days per week which had a rest on Sunday, and one time every day in the morning. They trained 40 min at the first week, and then the time was added to 50 min at the second week,60 min at the last week. The speed of running on the first day was 20 m/min, then, it was increased every two days, and reached 38 m/min in the end. Group T was taken nutrition supplement 15 min before the exercise and 15min after. Group S was the same all, but the supplement was water. Besides, group C was taken the equal water as group S. They were all put to death with 2% sedative pentobarbital sodium after 3 weeks, took the blood, liver and rectus femoris of rats to test liver glycogen, muscle glycogen, glucose, INS, SDH and MDH, and used SPSS13.0 to dispose statistics.
Results:(1)Univariated general linear model analysis showed that the main effect nutrition supplement on the liver and muscle Glycogen storage and the level of blood Glucose, serum Insulin, rectus femoris Succinate Dehydrogenas and Malate Dehydrogenase was significant difference between the two groups (P<0.01). (2) One-Way ANOVA showed that the Muscle Glycogen at 6 and 24 hour after exercise was significantly higher than it at the next time within group S and T (P<0.01). The Liver Glycogen at 6 and 24 hour after exercise was significantly higher than it at the next time within group S and T (P<0.01). The Blood Glucose at 1 hour after exercise was significantly higher than it after exercise instantly of group S and T (P<0.01), the Blood Glucose at 6 and 24 hour after exercise was significantly higher than it at 1 hour after exercise of group S and T. The Insulin at 24 hour after exercise was significantly higher than it after exercise instantly and at 1 hour and 6 hour after exercise of group S and T (P<0.01). The rectus femoris Succinate Dehydrogenas at 6 hour after exercise was significantly higher than it after exercise instantly of group S and T. The rectus femoris Malate Dehydrogenase at 6 hour after exercise was significantly higher than it after exercise instantly of group T (P<0.05). (3) T test indicated that the level of Liver Glycogen of group T was significantly higher than that of group S at 6 and 24 hour after exercise (P<0.05). The level of Liver Glycogen of group T was significantly higher than that of group S, after exercise instantly and at 6 hour after exercise (P<0.05). The level of Blood Glucose of group T was significantly higher than that of group S at 1 and 6 hour after exercise (P<0.05). The level of Insulin of group T was significantly higher than that of group S at 1 and 24 hour after exercise (P<0.05). The level of rectus femoris Succinate Dehydrogenas and Malate Dehydrogenase of group T was significantly higher than that of group S after exercise instantly (P<0.05).
Conclusion:(1) Taking nutrition supplements of the rats can significantly enhance the capacity of the liver and muscle glycogen recovery, can improve the serum level of insulin secretion after exercise, and can slower decline the level of blood glucose to maintain it density. (2) Taking nutrition supplements of the rats can significantly enhance the capacity of the liver and muscle glycogen and blood glucose recovery at 6 hour after exercise. (3) Taking nutrition supplements of the rats can significantly disease the level of rectus femoris Succinate Dehydrogenas in cytoplasm and increase the level of rectus femoris Malate Dehydrogenase to enhance the energy supply after exercise instantly.
引文
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