壳聚糖衍生物和聚乙二醇双丙烯酸酯混合材料的制备及其性能表征
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摘要
壳聚糖(Chitosan)是甲壳类动物、菌类和昆虫壳骨架中提取物甲壳素的脱乙酰产物,是自然界中存在的唯一碱性多糖。壳聚糖具有良好的生物相容性、可降解性、抗菌消炎和促进伤口愈合等性能,近年来在生物医学领域越来越受到青睐,以壳聚糖为主要原料制备的人工皮肤与创面的贴合性能好,既透气、又吸水,不仅有抑菌消炎作用,而且具有抑制疼痛、止血和促进伤口愈合的功能。壳聚糖具有良好的成膜性,且容易改性,引入各种功能基团,壳聚糖衍生物在医药领域都有很广阔的应用前景。我们通过条件温和的迈克尔加成反应制备了两种壳聚糖衍生物-壳聚糖/聚乙二醇双丙烯酸酯和巯基壳聚糖,分别制备了流延壳聚糖复合膜和水凝胶材料,并利用红外光谱、扫描电镜、X射线衍射、力学性能、细胞毒性等测试,探究其结构和功能之间的关系。
具体内容和结论如下:
1.合成壳聚糖/聚乙二醇双丙烯酸酯衍生物,并制备了不同质量比的壳聚糖/聚乙二醇双丙烯酸酯混合膜,测试表明,该混合膜具有良好的机械性能、吸水性。间接细胞毒性结果表明,该混合膜对L929细胞不显示毒性,有很好的细胞相容性。细胞培养结果表明,混合膜能很好地促进细胞的贴附和生长繁殖。该膜材料适合用作伤口敷料。
2.利用光聚合技术,制备了巯基壳聚糖/聚乙二醇双丙烯酸酯水凝胶。首先探索了巯基壳聚糖合成反应的条件,然后对水凝胶材料进行了研究。结果表明该水凝胶具有良好的吸水性。扫描电镜观察到水凝胶表明具有良好的孔洞结构,间接细胞毒性结果表明,该水凝胶材料对L929细胞不显示毒性,有较好的细胞相容性,可以作为伤口敷料。
Chitosan is obtained by alkaline deacetylation of chitin from crustacean species, fungi and insects etc., the only one of natural alkaline polysaccarides in the world. Due to its biocompatibility, biodegradability, antibacterial and wound-healing activity, chitosan is currently receiving a great deal of interest for biomedical and pharmaceutical applications such as wound dressing, drug carrier and tissue engineered scaffolds and so on. In our study, two kinds of chitosan derivatives: chitosan/polyethyleneglycol diacrylate and thiol-chitosan were prepared by the mild Michael addition reaction. Then, these derivatives were used to prepared biomedical applications such as films、hydrogels as wound dressing material. And FTIR、SEM、XRD、mechanical property、MTT were used in order to find out the relationship of the property with the structure.
The main contents and conclusions are described as follows:
1. Synthesis of CS/PEGDA derivatives and biocompatible CS/PEGDA films with different weight ratio were prepared. The investigation show, those kinds of films has good mechanical property and water solubility. Meanwhile, potential uses of these films for wound dressing material were studied by examining its biodegradability, cytotoxicity and cellular compatibility. The results showed that these films had no cytotoxicity to L929 cell. Cell culture results showed that films were well in promoting the cell attachment and proliferation. The films could use as a wound dressing material.
2. Biocompatible photocrosslinkable thiol-chitosan/PEGDA hydrogels were synthesized by the photopolymerization technique. Firstly, the conditions for synthesis of thiol-chitosan and then some properties of hydrogels were study. The result show: the hydrogel had good water solubility. The SEM shows that the surface of the hydrogel has pore structure. Meanwhile, indirect cytotoxicity assessment of the hydrogels indicated that the hydrogels were nontoxic to L929 cell.
具体内容和结论如下:
1.合成壳聚糖/聚乙二醇双丙烯酸酯衍生物,并制备了不同质量比的壳聚糖/聚乙二醇双丙烯酸酯混合膜,测试表明,该混合膜具有良好的机械性能、吸水性。间接细胞毒性结果表明,该混合膜对L929细胞不显示毒性,有很好的细胞相容性。细胞培养结果表明,混合膜能很好地促进细胞的贴附和生长繁殖。该膜材料适合用作伤口敷料。
2.利用光聚合技术,制备了巯基壳聚糖/聚乙二醇双丙烯酸酯水凝胶。首先探索了巯基壳聚糖合成反应的条件,然后对水凝胶材料进行了研究。结果表明该水凝胶具有良好的吸水性。扫描电镜观察到水凝胶表明具有良好的孔洞结构,间接细胞毒性结果表明,该水凝胶材料对L929细胞不显示毒性,有较好的细胞相容性,可以作为伤口敷料。
Chitosan is obtained by alkaline deacetylation of chitin from crustacean species, fungi and insects etc., the only one of natural alkaline polysaccarides in the world. Due to its biocompatibility, biodegradability, antibacterial and wound-healing activity, chitosan is currently receiving a great deal of interest for biomedical and pharmaceutical applications such as wound dressing, drug carrier and tissue engineered scaffolds and so on. In our study, two kinds of chitosan derivatives: chitosan/polyethyleneglycol diacrylate and thiol-chitosan were prepared by the mild Michael addition reaction. Then, these derivatives were used to prepared biomedical applications such as films、hydrogels as wound dressing material. And FTIR、SEM、XRD、mechanical property、MTT were used in order to find out the relationship of the property with the structure.
The main contents and conclusions are described as follows:
1. Synthesis of CS/PEGDA derivatives and biocompatible CS/PEGDA films with different weight ratio were prepared. The investigation show, those kinds of films has good mechanical property and water solubility. Meanwhile, potential uses of these films for wound dressing material were studied by examining its biodegradability, cytotoxicity and cellular compatibility. The results showed that these films had no cytotoxicity to L929 cell. Cell culture results showed that films were well in promoting the cell attachment and proliferation. The films could use as a wound dressing material.
2. Biocompatible photocrosslinkable thiol-chitosan/PEGDA hydrogels were synthesized by the photopolymerization technique. Firstly, the conditions for synthesis of thiol-chitosan and then some properties of hydrogels were study. The result show: the hydrogel had good water solubility. The SEM shows that the surface of the hydrogel has pore structure. Meanwhile, indirect cytotoxicity assessment of the hydrogels indicated that the hydrogels were nontoxic to L929 cell.
引文
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