黄芪多糖对小鼠学习记忆能力的影响及其机制初探
摘要
[目的]观察黄芪多糖(Astragalus Polysaccharides, APS)对小鼠学习记忆能力的影响,并初步探讨其可能的生物化学机制。
[方法]将40只昆明小鼠随机分成4组,每组10只,即黄芪多糖小剂量组、中剂量组、大剂量组和对照组,分别灌胃APS200mg/kg、400mg/kg和800mg/kg,对照组灌等量的生理盐水(20.0ml/kg)。灌胃3W后进行Morris水迷宫实验,测量记录小鼠每次的上台潜伏期、总路程、穿越站台次数和站台象限停留时间,测定小鼠血清中的超氧化物歧化酶(Superoxide Dismutase, SOD)活性和丙二醛(Malonaldehyde, MDA)含量。
[结果]
1.黄芪多糖对小鼠一般状态和体重的影响：实验过程中,各组小鼠一般状态良好,黄芪多糖各剂量组与对照组比较,小鼠第3周末体重无明显差异(P>0.05)。
2.黄芪多糖对小鼠学习记忆能力的影响：①黄芪多糖中、大剂量组与对照组比较,小鼠的上台潜伏期缩短、总路程缩短(均P<0.05)；黄芪多糖小剂量组与对照组比较,小鼠的上台潜伏期和总路程无明显差异(均P>0.05)；②黄芪多糖中、大剂量组与对照组比较,小鼠的穿越站台次数增多和站台象限停留时间显著增加(均P<0.05)；黄芪多糖小剂量组与对照组比较,小鼠的穿越站台次数和站台象限停留时间无明显差异(均P>0.05)。
3.黄芪多糖对小鼠血清SOD、MDA含量的影响：黄芪多糖中剂量、大剂量组与对照组比较,小鼠血清中SOD活性增强、MDA含量显著降低(均P<0.05);黄芪多糖小剂量组小鼠血清中的SOD活性和MDA含量与对照组比较无明显统计学差异(均P>0.05)。
[结论]黄芪多糖能够增强小鼠的学习记忆能力,其作用机制可能与抗自由基作用有关。
Objective:To investigate effects and its biochemical mechanism of Astragalus Polysaccharides on learning and memory in mice.
Methods:Forty mice were randomly divided into four groups, control group, Astragalus Polysaccharides low dose group(200mg/kg),middle dose group(400mg/kg) and large dose group(800mg/kg). It is ten in every group. The control group was administrated by physiological saline. The ascend platform latent period, all path, crossing platform number of times and platform quadrant residence time were measured, after intragastric administration three weeks. Then, the blood were obtained by extirpating eyeball in mice. superoxide dismutase activity and malondialdehyde content were determined by centrifuging the blood.
Results:1. Effects of astragalus polysaccharides on common condition and weight in mice:After intragastric administration three weeks with Astragalus Polysaccharides in mice, the common contion were good. Moreover, all Astragalus Polysaccharides groups's mice weight hadn't significantly changed compaired with control group (P>0.05).
2. Effects of astragalus polysaccharides on learning and memory:①Astragalus Polysaccharides with middle dose and large dose can shorten ascend platform latent period and all path compaired with control group in mice (P＜0.05), but not in low dose group(P>0.05).②Astragalus Polysaccharides with middle dose and large dose can increase crossing platform number of times and platform quadrant residence time compaired with control group in mice (P＜0.05), but not in low dose group(P>0.05).
3. Effects of astragalus polysaccharides on SOD and MDA:Astragalus Polysaccharides with middle dose and large dose can increase SOD and degrade MDA compaired with control group (P＜0.05), but not in low dose group(P>0.05).
Conclusion:Astragalus Polysaccharides can enhance the ability of learning and memory in mice. Its mechanism of action maybe concerned with enhancing anti-free radical in blood serum.
[方法]将40只昆明小鼠随机分成4组,每组10只,即黄芪多糖小剂量组、中剂量组、大剂量组和对照组,分别灌胃APS200mg/kg、400mg/kg和800mg/kg,对照组灌等量的生理盐水(20.0ml/kg)。灌胃3W后进行Morris水迷宫实验,测量记录小鼠每次的上台潜伏期、总路程、穿越站台次数和站台象限停留时间,测定小鼠血清中的超氧化物歧化酶(Superoxide Dismutase, SOD)活性和丙二醛(Malonaldehyde, MDA)含量。
[结果]
1.黄芪多糖对小鼠一般状态和体重的影响：实验过程中,各组小鼠一般状态良好,黄芪多糖各剂量组与对照组比较,小鼠第3周末体重无明显差异(P>0.05)。
2.黄芪多糖对小鼠学习记忆能力的影响：①黄芪多糖中、大剂量组与对照组比较,小鼠的上台潜伏期缩短、总路程缩短(均P<0.05)；黄芪多糖小剂量组与对照组比较,小鼠的上台潜伏期和总路程无明显差异(均P>0.05)；②黄芪多糖中、大剂量组与对照组比较,小鼠的穿越站台次数增多和站台象限停留时间显著增加(均P<0.05)；黄芪多糖小剂量组与对照组比较,小鼠的穿越站台次数和站台象限停留时间无明显差异(均P>0.05)。
3.黄芪多糖对小鼠血清SOD、MDA含量的影响：黄芪多糖中剂量、大剂量组与对照组比较,小鼠血清中SOD活性增强、MDA含量显著降低(均P<0.05);黄芪多糖小剂量组小鼠血清中的SOD活性和MDA含量与对照组比较无明显统计学差异(均P>0.05)。
[结论]黄芪多糖能够增强小鼠的学习记忆能力,其作用机制可能与抗自由基作用有关。
Objective:To investigate effects and its biochemical mechanism of Astragalus Polysaccharides on learning and memory in mice.
Methods:Forty mice were randomly divided into four groups, control group, Astragalus Polysaccharides low dose group(200mg/kg),middle dose group(400mg/kg) and large dose group(800mg/kg). It is ten in every group. The control group was administrated by physiological saline. The ascend platform latent period, all path, crossing platform number of times and platform quadrant residence time were measured, after intragastric administration three weeks. Then, the blood were obtained by extirpating eyeball in mice. superoxide dismutase activity and malondialdehyde content were determined by centrifuging the blood.
Results:1. Effects of astragalus polysaccharides on common condition and weight in mice:After intragastric administration three weeks with Astragalus Polysaccharides in mice, the common contion were good. Moreover, all Astragalus Polysaccharides groups's mice weight hadn't significantly changed compaired with control group (P>0.05).
2. Effects of astragalus polysaccharides on learning and memory:①Astragalus Polysaccharides with middle dose and large dose can shorten ascend platform latent period and all path compaired with control group in mice (P＜0.05), but not in low dose group(P>0.05).②Astragalus Polysaccharides with middle dose and large dose can increase crossing platform number of times and platform quadrant residence time compaired with control group in mice (P＜0.05), but not in low dose group(P>0.05).
3. Effects of astragalus polysaccharides on SOD and MDA:Astragalus Polysaccharides with middle dose and large dose can increase SOD and degrade MDA compaired with control group (P＜0.05), but not in low dose group(P>0.05).
Conclusion:Astragalus Polysaccharides can enhance the ability of learning and memory in mice. Its mechanism of action maybe concerned with enhancing anti-free radical in blood serum.
引文
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