鸡精液稀释液设计与筛选方法学研究
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摘要
迄今为止,国内外虽有不少有关鸡精液稀释液研究的相关报道,但关于精液稀释液设计与筛选方法学的研究尚属缺乏。在这一领域没有一套完整而实用的理论体系,对诸如精液稀释液pH值、渗透压、缓冲能力和配方物质组成进行调控、筛选和评价,如缓冲能力就没有具体参数可供借鉴,也缺乏测定方法等。有鉴于此,本论文拟从方法学的角度,探索鸡精液稀释液设计与筛选方面的指标体系和测定方法,用以指导制备高受精率的鸡精液稀释液,以适应现代养鸡生产的需要。
本论文包括三个部分,第一部分是鸡精液稀释液设计与主要液体质量指标的确定、控制与测定研究。对精液稀释液pH值、渗透压和缓冲能力梯度进行理论控制,然后以实际测定结果检验控制的准确性。第二部分是鸡精液稀释液实验室筛选研究——稀释精液体外精子生存质量与形态学观察研究。通过研究不同pH值、渗透压、缓冲能力和溶质种类的稀释液以及保存时间和温度对保存于其中的精子形态和机能的影响,确定最佳稀释液的合理参数。第三部分是鸡稀释精液人工授精应用研究,以稀释精液进行人工授精,以受精率对稀释液及其设计和筛选方法的优劣做出客观的评价。
实验结果表明:
①pH值的理论控制准确率在98.69%,渗透压准确率在95.9%-99.1%,缓冲指数准确率在97.6%以上。
②在2℃-5℃低温条件下,稀释液缓冲指数不影响精子活率,但影响精子畸形率。缓冲指数348时,精子畸形率极显著地低于缓冲指数119.6～228.8和461.6～1147.6范围的畸形率。为了保持精子正常的形态,不但要求要有合适的pH值和渗透压,同时还要求有适中的缓冲指数。稀释液缓冲指数对精子畸形率的影响较精子活率的影响大。
③柠檬酸钠、葡萄糖对鸡精液的保存,具有重要作用,而氯化钾在鸡精液稀释液中并不是必需的;复方稀释液保存效果普遍优于单方稀释液;低温下鸡精液稀释液渗透压应为等渗或接近等渗,且最适渗透压还与构成稀释液的物质种类和相互配比有关系;稀释液PH值应接近中性,最好与精液pH值保持一致;在缓冲指数为3.894-7.257的范围内,缓冲指数与精子保存效果的两个指标均呈现显著的负相关关系。
④精子最高生存指数出现在三种有机盐的稀释液中;两种无机盐液中精子存活时间最长,但精子畸形率很高;渗透压值与精子头部畸形类型高度相关(p<0.01),与精子头部弯曲且膨胀百分数的相关系数为-0.93,与精子头部单纯弯曲百分数的相关系数为0.94。在300mmol/L时,精子生存指数、存活时间均较好;500mmol/L时精子畸形率最低;渗透压对保存效果有极显著的影响,物质种类对保存48h精子形态有显著地影响,且渗透压与精子畸形类型有一
So far, there are many studies on chicken semen extender, but the study on the methodology of design and riddling of it is a gap. There is not integrated and applied system on this field, so that the pH,osmotic pressure (OP), buffer capacity (BC) and material of semen extender were controlled , riddled and valued, for example, there is not idiographic parameter on the BC used for reference and there is not measure method, too. So the index system and measure method of chicken semen extender design and riddling were researched in present study on the methodology point of view, in order to confect semen extender that lead to high fertility and adapt the need of modern chicken production.
The present study was divided to three parts. The first part included designing of chick semen extender and ascertaining, controlling and measuring the solution quality. The pH, OP and buffer capacity grads (BCA) of semen extender were controlled theoretically, and then exam the nicety rate according the result measured actually. The second part of the study was determining the composition of extender in lab by sperm quality and morphology in it in vitro. The sperm was stored in extenders which their pH,OP,BCA were different for varied time and temperature, and observed the effect on sperm, in order to determine the optimal parameter of the optimized semen extender. The third part was the research of application of AI using diluted semen. According to AI in practically production and the fertility rate of eggs,the extenders were valued objectively.
The results showed that:
①The nicety rate of the pH was 98.69% and the osmotic pressure was 95.99%–99.1%, the grads of buffer index of extenders were more than 97.6%.
②The buffer index (BI) did not affect the motility of sperm, but did the abnormality ratio. The abnormality ratio was lower significantly when the buffer index was 348 than 119.6～228.8 and 461.6～1147.6.In order to keep the normal shape of sperm, the pH and osmotic pressure should be suitable, at the same time, the buffer index also be moderate. The effect of buffer index on abnormality ratio was greater than sperm motility.
③First, sodium citrate, glucose play an important role in chick semen extender, but the Potassium chloride (PCH) is not necessary. Second, compound extender is better than the simple one. Third, at low temperature, the osmotic pressure should be equal the osmotic pressure of cock semen or be close to it, what is more, the optimum osmotic pressure is related with the material that compose extender and their proportion in it. Fourth, the pH should be litmusless or be same with semen. Fifth, when buffer index range from 3.894 to 7.257, there are significant negative correlation between buffer index and the two index of semen storage.
④The tiptop survival index of sperm showed in three kinds of organic salt extenders; the longest survival time appears in two inorganic extenders, but the monstrous sperm is more; in the extender containing sucrose and monosodium glutamate (MG), the abnormality sperm only 5.29% and 5.40%; there are high correlation between osmotic pressure and monstrous sperm head (p<0.01), the correlation coefficient was -0.93 with flexural and bulgy sperm head , and 0.94 with only flexural one. When the concentration was 300mmol/L, sperm survival index (SSI) and time (SST) were better; and monstrous sperm was the lowest when 500mmol/L. The effect of osmotic pressure on
本论文包括三个部分,第一部分是鸡精液稀释液设计与主要液体质量指标的确定、控制与测定研究。对精液稀释液pH值、渗透压和缓冲能力梯度进行理论控制,然后以实际测定结果检验控制的准确性。第二部分是鸡精液稀释液实验室筛选研究——稀释精液体外精子生存质量与形态学观察研究。通过研究不同pH值、渗透压、缓冲能力和溶质种类的稀释液以及保存时间和温度对保存于其中的精子形态和机能的影响,确定最佳稀释液的合理参数。第三部分是鸡稀释精液人工授精应用研究,以稀释精液进行人工授精,以受精率对稀释液及其设计和筛选方法的优劣做出客观的评价。
实验结果表明:
①pH值的理论控制准确率在98.69%,渗透压准确率在95.9%-99.1%,缓冲指数准确率在97.6%以上。
②在2℃-5℃低温条件下,稀释液缓冲指数不影响精子活率,但影响精子畸形率。缓冲指数348时,精子畸形率极显著地低于缓冲指数119.6～228.8和461.6～1147.6范围的畸形率。为了保持精子正常的形态,不但要求要有合适的pH值和渗透压,同时还要求有适中的缓冲指数。稀释液缓冲指数对精子畸形率的影响较精子活率的影响大。
③柠檬酸钠、葡萄糖对鸡精液的保存,具有重要作用,而氯化钾在鸡精液稀释液中并不是必需的;复方稀释液保存效果普遍优于单方稀释液;低温下鸡精液稀释液渗透压应为等渗或接近等渗,且最适渗透压还与构成稀释液的物质种类和相互配比有关系;稀释液PH值应接近中性,最好与精液pH值保持一致;在缓冲指数为3.894-7.257的范围内,缓冲指数与精子保存效果的两个指标均呈现显著的负相关关系。
④精子最高生存指数出现在三种有机盐的稀释液中;两种无机盐液中精子存活时间最长,但精子畸形率很高;渗透压值与精子头部畸形类型高度相关(p<0.01),与精子头部弯曲且膨胀百分数的相关系数为-0.93,与精子头部单纯弯曲百分数的相关系数为0.94。在300mmol/L时,精子生存指数、存活时间均较好;500mmol/L时精子畸形率最低;渗透压对保存效果有极显著的影响,物质种类对保存48h精子形态有显著地影响,且渗透压与精子畸形类型有一
So far, there are many studies on chicken semen extender, but the study on the methodology of design and riddling of it is a gap. There is not integrated and applied system on this field, so that the pH,osmotic pressure (OP), buffer capacity (BC) and material of semen extender were controlled , riddled and valued, for example, there is not idiographic parameter on the BC used for reference and there is not measure method, too. So the index system and measure method of chicken semen extender design and riddling were researched in present study on the methodology point of view, in order to confect semen extender that lead to high fertility and adapt the need of modern chicken production.
The present study was divided to three parts. The first part included designing of chick semen extender and ascertaining, controlling and measuring the solution quality. The pH, OP and buffer capacity grads (BCA) of semen extender were controlled theoretically, and then exam the nicety rate according the result measured actually. The second part of the study was determining the composition of extender in lab by sperm quality and morphology in it in vitro. The sperm was stored in extenders which their pH,OP,BCA were different for varied time and temperature, and observed the effect on sperm, in order to determine the optimal parameter of the optimized semen extender. The third part was the research of application of AI using diluted semen. According to AI in practically production and the fertility rate of eggs,the extenders were valued objectively.
The results showed that:
①The nicety rate of the pH was 98.69% and the osmotic pressure was 95.99%–99.1%, the grads of buffer index of extenders were more than 97.6%.
②The buffer index (BI) did not affect the motility of sperm, but did the abnormality ratio. The abnormality ratio was lower significantly when the buffer index was 348 than 119.6～228.8 and 461.6～1147.6.In order to keep the normal shape of sperm, the pH and osmotic pressure should be suitable, at the same time, the buffer index also be moderate. The effect of buffer index on abnormality ratio was greater than sperm motility.
③First, sodium citrate, glucose play an important role in chick semen extender, but the Potassium chloride (PCH) is not necessary. Second, compound extender is better than the simple one. Third, at low temperature, the osmotic pressure should be equal the osmotic pressure of cock semen or be close to it, what is more, the optimum osmotic pressure is related with the material that compose extender and their proportion in it. Fourth, the pH should be litmusless or be same with semen. Fifth, when buffer index range from 3.894 to 7.257, there are significant negative correlation between buffer index and the two index of semen storage.
④The tiptop survival index of sperm showed in three kinds of organic salt extenders; the longest survival time appears in two inorganic extenders, but the monstrous sperm is more; in the extender containing sucrose and monosodium glutamate (MG), the abnormality sperm only 5.29% and 5.40%; there are high correlation between osmotic pressure and monstrous sperm head (p<0.01), the correlation coefficient was -0.93 with flexural and bulgy sperm head , and 0.94 with only flexural one. When the concentration was 300mmol/L, sperm survival index (SSI) and time (SST) were better; and monstrous sperm was the lowest when 500mmol/L. The effect of osmotic pressure on
引文
[1]Bakst,M.R.,G.Mishart,and J.P.Brillard, Oviducal sperm selection,transport, and storage in poultry[J].Poult.Sci.Rev.1994,5:117-143
[2]Froman,D.P., and D.J.McLean, Objective measurement of sperm motility based upon sperm penetration of Accudenz[J].Poultry Sci.1996,75:776-784
[3]Proman,D.P.,A.J.Feltmann,and D.J.McLean, Increased fecundity resulting from semen donor selection based upon in vitro sperm motility. Poultry Sci.1997, 76:73-77
[4]Allen,C.J.,and L.R. champion, Competitive fertilization in the fowl[J].Poultry Sci.1995,34:1332-1342
[5]Cooper,D.M.,and J.G.Rowell, Relations between fertility,embryonic survival and some semen characteristics in the chicken[J].Poultry Sci.1958.37:699-707
[6]Kamar,G.A.R., The influence of semen characteristics on hatching results of chicken eggs.Poultry Sci.1960,39:199-192
[7]McDaniel,G.R.,and J.V.Craig, Predicting male fertilizing capacity in high and low fertility strains of chickens[J].Poultry Sci.1962,41:866-869
[8]Wishart,G.J.,and F.H.Palmer, Correlation of the fertilizing ability of semen from individual male fowls with sperm motility and ATP cointent[J].Poultry Sci. 1986,27:97-102
[9]Ashizawa,K.,and R.Sano, Effects of temperature on the immobilization and the initiation of moyility of spermatozoa in the male reproductive tract of the domestic fowl,Gallus domesticus[J].Comp.Biochem.Physiol.1990,96A:297-301
[10]Amann,R.P.,and R.H.Hammerstedt, In vitro evaluation of sperm quality:An opinion[J].Androl.1993,14:397-406
[11]Wilson,H.R.,N.P.Piesco,E.R.Miller,and W.G.Nesbeth, Prediction of the fertility potential of broiler breeder males[J].World’s Poult.Sci. J. 1979, 35:95-118
[12]Bakst,M.R.,and H.C. cecil, Techniques for semen evaluation,semen storage,and fertility determination[J].Poultry Science Association, Inc., Savoy,IL. 1997.
[13]Bilgili,S.f.,and J.A.Renden,Fluorometric determination of avain sperm viability and concentration[J].Poultry Sci.1984,63:2275-2277
[14]McDaniel,C.D.,J.L.Hannah,H.M.Parker,C.D.Schultz,and C.D.Zumwalt, The relati- onship of the sperm motility index with rooster sperm quality and fertilizing ability[J].Poultry Sci.1997,76(Suppl.1):115.(Abstr.)
[15]Wishart,G.J.,and Y.I.Wilson Sperm motility and metabolism IV.Spermmotility analysis using the “Sperm quality analyzer”.Pages54-55 in: Techniques for semen evaluation,semen storage,and fertility determination. M.R.Bakst and H.C.Cecil, ed.Poultry Science Association,Savoy,IL. 1997.
[16]Bartoov,B.,J.Ben-Barak,A.Mayevsky,M.Sneider,L.Yogev,and A.Lightman, Sperm motility index :a newparameter for human sperm evaluation[J]. Fertil. 1991, 56:108-112
[17]Johnston,R.C.,G.N.Clarke,D.Y.Liu,and H.W.G.Bake, Assessment of the sperm quality analyzer[J].Fertil.Steril.1995,63:1071-1076
[18]Matilsky,M.,M.BEN-Ami,V.Eyali,Y.Geslevieh,Y.BEN-Barak,and E.Shalev, Correlation between sperm motility index as measured by the sperm quality analyzer and the outcome of intrauterine inseminations.Human Reprod. 1993, 8(Suppl.1): 98.(Abstr.)
[19]McDaniel,C.D.,J.L.Hannah,H.M.Parker,T.W.Smith,C.D.Schultz,and C.D.Zumwalt, Use of a sperm analyzer for evaluating broiler breeder males.1.Effects of altering sperm quality and quantity on the sperm motility index[J].Poultry Sci. 1998,77: 888-893
[20]Guyton,A.C.,Page 890 in:Textbook of Medical physiology.8th ed.W.B.Saunders Co.,Philadelphia,PA.1991.
[21]Clarke,R.N.,T.J.Sexton,and M.A.Ottinger, Effects of holding temperature and storage time on respiratory rate,motility,and fertility of chicken and tuekey semen[J].Poultry Sci.1982.61:1912-1917
[22]Sexton , T.J. , Preservation of poultry semen.A review.Pages 159-170 in: Beltsville Symposia in Agricultural Research. 3.Animal Reproduction Allanheld,Osmun and Co.,Montclair,NJ.1979.
[23]Wishart,G.J., The effect of anoxia on fowl spermatozoa:Recovery of fertilizingability,motility and cellular concentrations of potassium and ATP[J].Gamete Res. 1984,10:83-89
[24]Vantman,D.,S.M.Banks,G.Koukoulis,L.Dennison,and R.J.Sherins, Assessment of sperm motion characteristics from fertile and infertile men using a fully automated computer-assissted semen analyzer[J].Fertil.1989,51:156-161
[25]Froman,D.P.,and D.J.McLean, Objective measurement of sperm motility based upon sperm penetration of Accudenz[J].Poultry Sci.1996,75:776-784
[26]Bakst,M.R.,G.Wishart,and J.P.Brillard, Oviducal sperm selection, transport, and storage in poultry[J].Poult.Sci.Rev.1994,5:117-143.
[27]Thomson,M.F., and G.j.Wishart, Elucidation of the mechanism responsible for the temperature-dependent reversible inactivation of the motility of fowl spermatozoa.Br[J].Poult.Sci.1988,30:687-692.
[28]Ashizawa,K.,S. Maeda,and K.Okauchi, The mechanisms for reversible immobilize- ation of fowl spermatozoa at body temperature[J]. Reprod. Fertil.1989,86: 271-276.
[29]Ashizawa,K.,A.Hashiguchi,and Y.Tsuzuki,Intracellular free Ga2+ concentration in fowl spermatozoa and its relationship to motility and respiration in spermatozoa.[J].Reprod.Fertil.1992,96:395-405.
[30]Thoumon,M.F.,and G.J.Wishart, Temperature-mediated regulation of calcium flux and moutility in fowl spem[J]. Reprod.Fertil.1991,93:385-391.
[31] Ashizawa,K.,H.Tomonaga,and Y. Tsuzuki, Regulation of flagellar motility of fowl spermatozoa: evidence for the involvement of intracellular Ga2+ and calmodulin [J].Reprod.Fertil.1994,101:265-272.
[32]Ashizawa,K.,and G. J. Wishart, Resolution of the sperm motility- stimulating principle of fowl seminal plasma in Ga2+ and an unidentified low molecular Weight factor[J].Reprod.Fertil.1987,81:495-499.
[33] Bakst M.R., and H.C.Cecil, Effect of bovine serum albumin on motility and fecundity of turkey spermatozoa before and after storage. J. Reprod.Fertil. 1992,94:287-293.
[34]Mohau, J.,M. Saini, and P. Joshi, Isolation of a spermatozoal motility inhib1ting factor from chicken seminal plasm with antibacterial property[J].Biochem. Biophys.Acta.1995,1245:407-413.
[35]Lake,P.E., The male in reproduction. Pages 381-405 in: Physiology and Biochemi- stry of the Domestic Fowl.Vo1.5.B.M.Freeman,ed. Academic Press, London, UK.1984.
[36]Lesslley,B.A.,and K. I. Brown,.Purification and properties of a proteinase inhibitor from chicken seminal plasma[J].Biol.Reprod.1978,19:223-234.
[37]Freeman,B. M., Appendix:Biochemical and physiological data. Pages 407-424 in: Physiology and Biochemistry of the Domestic Fowl.Vol.5.B.M.Freeman and D.J.Bell, ed. Academic Press, London, UK.1984.
[38]Froman,D.P., and K. A. Thursam, Desialylation of the rooster sperm's glycocalyx decreases sperm sequestration following intravaginal insemination of the hen. Biol.Reprod.1994,50:1094-1099.
[39]Wishart,G.J.,and K.Ashizawa, Regulation of the motility of fowl spermatozoa by calcium and cAMP .J.Reprod.Fertil.1987,80:607-611.
[40]Goldberg,E.,and C.Norman, The metabolism of ejaculated spermatozoa from the fowl[J].Cell Comp.Physiol.1961,58:175-180.
[41]Sexton , T.J. , Oxidative and glycolytic activity of chicken and turkey spermatozoa[J]. Comp. Biochem.Physiol.1974,488:59-65.
[42]Wishart,G.J., Maintenance of ATP concentrations in and of fertilizing ability of fowl and turkey spermatozoa in vitro[J]. Reprod.Fertil.1982,66:457-462.
[43]Howarth,B.,Jr., Oxidation of [U-14C] palmitic acid by cock spermatozoa[J]. Poultry Sci.1978,57:1751-1754.
[44]Howarth,B,Jr., The phospholipid profile of cock spermatozoa before and after in vitro incubation for twenty-four hours at 41℃[J]. Poultry Sci.1981,60: 1516-1519.
[45]N.Fujihara [J]World`s Poultry Science Journal 1992, Vol.48 No.1,39-55
[46]Bogdonoff,P.D.,and C.S.Shaffner,The effect of pH on the in vitro survival, metabolic activity,and fertilizing capacity of chicken semen[J].Poultry Sci., 1954,33:665-669
[47]Dunnett, C.W..A multiple compartson procedure for comparing several treatments with acontrol[J].Amer.Statist-Assoc.1955,50:1096-1121.
[48]Fewlass,T.A.,T.J.Sexton and C.S.Shaffner. Effect of various levels of egg yolk,milk,seminalplasma or blood serum on the respiration andreproductive efadeney of chicken spermatozoa[J]. Poultry Sci.1975,54:346-349.
[49]Flipse,R.J.,S.Patton and J.O.AImquist, Dilutors for bovine semen.III.Effect of lactenin and lactoperoxidase upon spermatozoa livability[J].Dairy Sci.1954, 37:1205-1211.
[50]Hobbs,T.D.,and G.C. Harris,Effect of freezing point depression and CO2 on motility and fertility of chicken spermatozoa stored in carbondioxide extenders[J].Poultry Sci.1963,42:388-393.
[51]Lake,P.E..Studies on the dilution and storage of fowl semen[J]. Reprod. Fem. 1960,1:30-35.
[52]Lake,P.E.,and W.M.Mcindoe,The glutamic acid and creatine content of cock seminal plasma[J].Biochem.1959,J.71:303-306.
[53]Ogasawara,F.X.,and R.A.Ernst.Recent developments in the artificial insemin- ation of turkeys[J].Feedstuffs.1975,47:23-35.
[54]Polge,C.,Artificial insemination in the fowl[C].Proc.Soc.Study Fert.1950, 2:16-22.
[55]Schindler,H.,S.Weinstein,E.Moses and I Gabriel.The effect of cock semen. Poultry Sci.1955,34:1113-1117.
[56]Sexton,T.J.Oxidative glycolytic acdvity of chicken and turkey spermatozoa[J]. Comp.Biochem.Physiol.1974,48B:59-65.
[57]Sexton,T.J..Studies on the dilution of turkey semen[J].Poultry Sci.1976, 17:179-184.
[58]Shannon,P..Presence of a heat labile toxic protein in bovine seminal plasma[J]. Dairy Sci.1965,48:1362-1366.
[59]Smith,A.U.,1949.The control of bacterial growth in fowl semen[J].Ag.Sci.1949, 39:194-200.
[60]Van Tienhoven,A.,R.G.D.Steel and S.A.Duchaine.Diluents for turkey semen[J]. Poultry Sci.1958,37:47-53.
[61]Van Wambake,F..The storage of fowl spermatozoa.I.Preliminary results with diluents[J]. Reprod.Fert.1967,13:571-575.
[62]Wales , R.G. , and I. G.White.The viability of fowl spermatozoa in dilute suspension[J].AustralianJ. Biol.Sci.1961,14:637-645.
[63]Weakly,C.E.,and C.S.Shaffner.1952.The fertilizing capacity of diluted chicken semen[J].Poultry Sci.1952,32:650-653.
[64]White,I.G..The effect of potassium on the washing and dilution of mammalian spermatozoa.Australian [J].Exp.Biol.1953,31:193-200.
[65]Wilcox,F.H..The effect of different hydrogenion concentrations during storage and at insemination and of added magnesium and potasium on the fertilizing ability of chicken semen[J].Poultry Sci.1959,38:1159 一 1162.
[66]Wilcox,F.H. Effect on fertility of temperature handling methods,Lake's solution,and the addition of egg white,egg yolk and sugars to the diluent used in Storing chicken semen[J].pou1try Sci.1960,39:459-467.
[67]Wilcox,F.H.,and M.S.Shorb.The effect of antibiotics on bacteria in semen and on motility and fertilizing ability of chicken spermatozoa.Amer.J.Vet.Res. 1958,19:945-949.
[68]Yamane,J.,s.Tsukanaga and T.Takahashi.A basic principle of make-up of the dilutor for fowl semen[J].Zootech.Vet.1692,17:523-527.
[69]Sexton.T.J,T.A.Fewlass.A new poultry extender 2.effect of the diluent components on the fertilizing capacity of chicken semen stored at 5℃[J].Poultry Sci.1977,56:277-284.
[70]Sexton,T.J. Comparison of commercial diluents for holding turkey semen 24 hours at 5C[J].Poultry Sci .1988a,67:131-134.
[71]Lake,P.E. Recent progress in poultry reproduction[J].World’s Poult.Sci.J. 1989,45:53-59
[72]Sexton,T.J. Research note:Influence of damaged spermatozoa on the fwertility of turkey of turkey semen stored for 24 hours at 5C[J].Poultry Sci.1988b, 67: 1483-1485.
[73]Wishart, G.J., Physiological changes in fowl and turkey spermatozoa during in vitro storage[J]. Br.poult .sci.1989,30:443-454.
[74]Bakst.M.R.H.C.Cecil.snd T.J.Sexton, Modeficatiion of the ethidium bromide exclusion procedure for evaluation of turkey sperm[J].Poultry Sci.1991 ,70:336-370.
[75]Bakst M.R.G. Wishart.and J.P.Brillard . Oviductal sperm selection transport and storage in poultry[J]. Poult.Sci.Rev.1994,5:117-143,
[76]Thurston ,R,J., Storage of poultry semen above freezing for 24-48 hours .pages 107-122 in :proceedings of the First international symposium on the artificial Insemination of poultry .M.R.Bakst and G.J.wishart , ed poultry science Association ,savoy IL.1995.
[77]Wishart ,G.J., The effect of continuous aeration on the fertility of fowl and turkey semen stored above 0C[J].poult.sci.1981,22:445-450
[78]Sexton,T.J.and A.F.Giesen, Beltsville Poultry Semen Extender.6.Hloding turkey semen for six hours at 15C[J].Poultry Sci.1982,61:1202-1208.
[79]Jones,R,and T.Mann, Toxicity of sxogenous fattu acid peroxides towards spermatozoa[J].Reprod .Fertil.1973,50:255-260
[80]Jones,R.,and T.Mann, Toxicity of exogenous fatty acid peroxides towards spermatozoa[J].Reprod.Fertil.1977,50:255-260.
[81]Fujihara,N.and B.Howarth,Jr. Lipid peroxidation in fowl spermatozoa[J].Poultry Sci.1978,57:1766-1768.
[82]Fujihara,N.and O.Koga, Prevention of the production of lipid peroxide in rooster spermatozoa[J] .Anim.Reprod Sci.1984,7:385-390
[83]Wishart ,G.J., Effects of lipid peroxide formation in fowl semen on sperm motility .ATP content and fertilizing ability[J].Reprod . Fertil .71:113-118.
[84]Ravie,O,and P.E.Lake, The phospholipids –bound fatty acids of fowl and turkey spermatozoa[J]. Anim.Reprod.Sci.1984,9:189-192.
[85]Alvarez.J.G.and B.T.Storey, Role of superoxide dismutase in protecting rabbit spermatozoa from O2 toxicity due to lipid perpxodatoon[J]. Biol.Reprod. 1983,28:1129-1136.
[86]Alvarez.J.G.C.Touchstone,L.Blasco,and B.Storey, Spontaneous lipid peroxide- atong and production of hydrogen peroxide and superoxide in human soermatozoa superoxide dismutase as major enzyme p[rotectant against oxygen toxicity[J]. Androl.1987,8:338-348.
[87]Aitlen,R.J.S.Clarkson. Significance of reactive oxygen species and antioxidantsin defining the efficacy of spermatozoa preparation techniques[J]. Androl. 1988, 9:367-376.
[88]Msxwell,W.M.C.and T. Stojanoc, Liquid storage of ram semen in the absence or presence of some antioxidants[J]. Report .Fertil.Dev.1996,8:1013-10120.
[89]Hammerstedt,R.H. Maintenance of bioenergetis balance in sperm and prevention of lipid peroxidation:A review of the effect on design of storage preservation systems[J].Reprod Fertil.Dev.1993,5:657-690.
[90]Blesboid E.I.Grasseau,and J.C.Blum, Effects of vitamin E on fowl semenstorageat 4C[J].Theriogenology 1993,39:771-779.
[91]Sural.P.F. Relations between vitamin E concentration in poultry spermatozoa and some semen biochemical and physiological characteristic .page 171-173 in : program of the 8th .International Symposia on Current problem in avian Genetics, Slovak academy science ,smolenice,slovakia.1989.
[92]Donoghue,A.M.D.L.Gatner,D.J.Donoghue.and L.A.Johson, Viability assessment of turkey sperm using fouo9rescent staining and golw cytometry[J].Poultry Sci. 1995,74:1191-1120.
[93]Donoghue A.M.D.L.Gatner D.J.Donoghue ,and L.Ajohnson, Assessment of the membrane integrity of fresh and stored turkey spermatozoa using a combination of hypoosmotis stress,fluorescent staining and flow cytometry[J]. Theriogenology 1996,46:153-163.
[94]Burton .G.W.and K.U.Ingold, Mechanisms of antioxidant action :prevention and chain –breaking antioxidants.pages 29-48 in:CRCHandbook of Free Radicals and Antiocidants in Biomedicine. Vol II.A.Maquel.T.Quintanilha . and.H.Weber, ed.Boca Taton.FL.1988.
[95]Fromen.D.P. and R.J.Thurston, Chicken and turkey spermatozoal superoxide dismutase:A comparative study[J]. Biol.Repord.1981,24:193-200.
[96]Cecil.H.C.and M.R.Bakst, Invitro lipid peroxidation of turkey spermatozoa[J]. Poultry Sci.1993,72:1370-1378.
[97]Smith, A. V., and C. Polge., Survival of spermatozoa at low temperatures[J]. Nature.1950,166-668.
[98]Ax, R. L., and J. R. Lodge, Rooster spermatozoa motility, forward progressionand fertility after storage of 25, 5, or –196C in various extenders[J]. Cryobiology. 1975,12:93-97.
[99]Polge, C., Functional survival of fowl spermatozoa after freezing at –79C[J]. Nature. 1951,167:949-950.
[100]Clark, C. E., and C.S. shaffner, The fertilizing capacity of frozen chicken sperm and the influence of related in vitro processes. Poultry Sci.1960,39: 1213-1222.
[101]Graham, E. F., and M. M. Pace, Some biochemical changes in spermatozoa due to freezing[J]. Cryobiology.1967,4:75-84.
[102]Brown, K. I., B.G. Crabarm, and M.M.Pace, Some factors affecting loss of intracellular enzymes from spermatozoa[J]. Cryobiology.1971,8:220-224.
[103]Sexton, T. J ., Comparison of various cryoprotective agents on washed chicken spermatozoa. 4. Metabolism and release of glutamic oxalacetic transaminase[J]. Poultry Sci.1974,53:284-287.
[104]Pace, M.M., and E.F. Graham, The release of glutamic oxaloacetic transaminase from bovine spermatozoa as a test method for assessing semen quality and fertility[J].Biol.Reprod.1970,3:140-146.
[105]Westfall, F.D., and G.C. Harris, Jr., The ability of cryopreservatives to prevent motility loss and freeze-thaw damage to the acrosome of chicken spermatozoa[J].Cryobiology.1975,12:89-92.
[106]Thurston, R. J., and G. C. Harris, Jr., Calcium-45 uptake as a measurement of freeze-thaw injury to chicken spermatozoa in the presence or absence of cryopreservatives[J].Cryobiology 1970,7:100-106.
[107]Allen, T.E., and L.W, The fertility of fowl spermatozoa in glycerol diluents after intrauterine insemination[J]. Poultry Sci.1995,34: 1167—1169.
[108]Westfall, F. D., and B, Howarth, J r. Duration of the antifertility effectof glycerol in the chicken vagna[J].Poultry Sci.1976,56:924-925.
[109]Marquez, B.J., and F. X., Ogasawara, Effects of glycerol on turkey sperm cell viability and fertilizing capacity[J]. Poultry Sci.1975, 54:1788(Abstr.)
[110] Westfall.F.D and Birkett howarth,JR. The effect of glycerol and dilution on the release of glutamic oxaloacetic transaminase from chicken spermatozoa[J].Poultry Sci.1978,57:1037-1041.
[111]Christensen, V.L., Diluents dilution and storage of turkey semen for six hours. Pages 90-106 in: First International Symposium on the Artificial insemination of poultry[R]. M.R.Bakst and G.J.Wishart, ed. Poultry Science Association, Savoy, IL.1995.
[112]Lake, P.E., Historical perspective of artificial insemination tech1nology. Pages 1-20 in: First Internationaal Symposium on the Artificial inseminaaon of Pourtry[C]. M.R.Bakst and G.J.Wishart,ed., Poultry Science Association, Savoy, IL.1995.
[113]Thurston,R.J., Storage of poultry semen above freezing for 24 to 48 hours[C]. Pages 107-122 in: First International Symposium on the ArtinciaI Insemination of Poultry. M.R Bakst and G.J.Wishad, ed. Poultry Science Association, Savoy, IL.1995.
[114]Leopold,A.C, Membranes,Metabolism and dry organism[M]. Cornell University Press, Ithaca, NY.1986.
[115]Ried,J.L.,and M.K.Walker-Simmons, Group 3 late embryogenesis abundant proteins in desiccation-tolerance seedings of wheat (Triticum aestivum L) [J]. Plant Physiol.1993,102:125-131.
[116]Churchill, T.A., and K.B.Storey, Dehydration tolerance in the wood frogs: a new perspective on development of amphibian freeze tolerance[J].Am.J.Phys. 1993,265:1324-1332.
[117]Kemode, A.R., Regulatory mechanisms in the transition from seed development to germination: Interractions between the embryo and he seed environment. Pages 273-332 in:Seed Development and Gemination[J].Kigel and G.Galili, ed., Marcel Dekker,Inc.,New York, NY.1995.
[118]Dure,L.,III,M.Crouch,J.Harada,T.H.D.Ho,Mundy,R.Quatrano,T.Thomas,and Z.R. Sung, Common amino acid sequences domains among the LEA proteins of higher plants-Plant[J]. Mol.Biol.1989,12:475-486.
[119]Jacobsen, J.V., and D.C Shaw, Heat-stable proteins and abscicic acid action in barley aleurone cells[J]. Plant Phys.1989,91:1520-1526.
[120]Ried,J.L.,and M.K. Walker-Simmons, Synthesis of abscisic acid-responsive, heat-stable proteins in embryonic axes of dormant wheat grain[J]. Plant Physiol.1990,93:6624
[121]Lin, W.W.Cuo, E.Everson, and M.F.Thomashow, Cold acclimation in Arabidopis and wheat. Aresponse associated with expression of related genesencoding “boiling-stable” polypeptides[J]. Plant Physiol.1990, 94:1078-1083.
[122]Walters,C.J., L.Reid, and M.K.Walker-Simmons, Heat-soluble proteins extracted from wheat embryos have tightly bound sugars and unusual hydration properties[J]. Seed Sci.Res.1997, 7:125-134.
[123] Lin,C.,and M.F.Thomashow, A cold-regulated Arabidopsis gene encodes a polypeptide having potent cryoprotectant activity[J]. Biochem. Biophys. Commun. 1992, 183:1103-1108.
[124]Luo,J., and B.M.Reed, Abscisic acid-responsive protein, bovine semen albumin, and praline pretreatments improverecovery of in vitro currant shoot-tip meristems and callus cryopreserved by vitrification[J]. Cryobiology 1997, 34:240-250.
[125]Donoghue,A.M.,M.K.Walker-Simmons, The influence of wheat dehydration-induced proteins on the function of turkey spermatozoa after twenty-four-hour in vitro storage[J]. Poultry Sci.1999, 78:235-241.
[126]Sexton,T.J., Comparison of commercial diluents for holding turkey semen 24 hours at 5℃[J]. Poultry Sci.1988,67:131-134.
[127]Bakst, M.R., and H.C.Cecil, Effect of bovine serum albumin on motility and fecundity of turkey spermatozoa before and after storage [J]. Reprod. Fertil. 1992, 94:287-293.
[128]Artus,N.N.,M.Uemura, P.L. Steponkus, S.J. Gilmour, C. Lin, and M.F.Thomashow, Constitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance[J].Proc.Natl. Acad.Sci.1996, 93:13404-13409.
[129]Wishart, G.J., Maintenance of ATP concentration in and of fertilizing ability of fowl and turkey sperm in vitro[J].J.Reprod.Fertil.1982,66:457-462.
[130]Irvine,D.S.,and R.J.Aitken, The value of adenosine triphosphate (ATP)measurements in assessing the fertilizability of human spermatozoa. Fertil- Steril.1985,44:806-813.
[131]Soderquist, L., H.Rodriguez-Martinez, and L. Janson, Post-thaw motility, ATP content and cytochrome C oxidase activity of AI bull spermatozoa in relation to fertility[J].Zentrabl.Vet.1991,38:165-174.
[132]Froman, D.P.,and D.J. McLean, Objective measurement of sperm motility based upon sperm penetration of Accudenz. Poultry Sci.1996,75:776-784.
[133]Donoghue,A.M.,D.R.Holsberger, D.P.Evenson, and D.P.Froman, Semen donor selection by in vitro sperm mobility increases fertility and sperm storage in the turkey hen[J].Androl.1998,19:295-301.
[134]Baks, M.R., G.Wishart, and J.P.Brillard, Oviductal sperm selection, transport and storage in poultry.Poult.Sci.Rev.1994,5:117-143.
[135]Brillard , J.P. , nd M.R. Bakst , Quantification of spermatozoa in the spermatozoa-storage tubules of turkey hens and the relation to spermatozoa numbers in the perivitelline layer of eggs[J]. Biol.Reprod.1990,43:271-275.
[136]Donogue, A.M., M.R.Bakst, D.R.Holsberger, and D.J.Donoghue, Effect of semen storage on sperm numbers in the perivitelline layer of laid turkey eggs[J].Reprod.Fetil.1995a,105:221-225.
[137]Donoghue, A.M.,D.L Garner, D.j.Donoghue, and L.A.Johnson, Viability assessment of turkey spermusing fluorescent staining and flow cytometry[J]. Poultry Sci.1995b, 74:1191-1200.
[138]Anderson, M.M., and C .P.Merilan, Dehydration studies on bovine spermatozoa. Pages 1-35in: Research Bulletin, University of Missouri Agricultural Experiment Station,Columbia,M0. 1960.
[139]Mazur,P., Freezing of living cells: Mechanisms and implications[J].Physiol. 1984,247:C125-142.
[140]Hammerstedt, R.H., J.K.Graham, and J.P.Nolan, Cryopreservation of mammalian sperm: What we ask them to survive[J].Andro1.1990,11:73-87.
[141]Dunnett,C.W..A Multiple comparison procedure for comparing several treatments with a control[J].Amer.Statist.Assoc.1955,50:1096-1121.
[142]Fewlass,T.A.,T.J.Sexton and C.S.Shaffner.Effect of various levels of egg yolk,milk,seminal plasma or blood serum on the respiration and reproductive efaciency of chicken spermatozoa[J].Pouley Sci.1975,54:346-349.
[143]Harris,G.C.,and T.D.Hobbs.The effects of fluid to gas ratio,dilution rate,and CO2 level on the fertilizing capacity of chicken spermatozoa stored in carbon dioxide extenders[J].Poultry Sci.1964,43:529-534.
[144]Harris,G.C.,and T.D.Hobbs.1968.Effects of freezing point depression and fluid to gas ratio on fertility on fowl spermatozoa stored in C02 extenders[J]. Reprod.Fert.1968,16:389-394.
[145]Sexton.T.J..A new poultry semen extender 3.Effect of storage conditions on the fertilizing capacity of chicken semen stored at 5℃[J].Poultry Sci.1977, 55: 285-289.
[146]Hunsaker,W.G.,and J.R.Aitken.The effect of visible light on the fertilizing capacity of fowl spermatozoa[J].Poultry Sci.1960,39:865-867.
[147]Sexton.T.J.A neu poultry semen extender 3.effect of storage conditions on the fertilizing capacity of chicken semen stored at 5℃[J]. Poultry Sci.1977, 55:285-289.
[148]Lake,P.E.. A retarding factor in the problem of fowl semen storage. proc.3rd Int.Congr.Anim.Reprod.Sec.1956,3:104-106.
[149]Lake,P.E.. Fowl semen as collected by the massage method.J. Ag.Sci.1958, 49:120-126.
[150]Norman,C.,and E.Goldberg. Effect of light on motility,life-sperm and respiration of bovine spepermatozoa[J].Science.1959,130:624-625.
[151]Norman,C.,E.Goldberg and I.D.Porterfield. The effect of visible radiation ofthe functional life-span of mammalian and avian spermatozoa Exp[J].Cell Res.1962,28:69-72.
[152]Pistenma,D.A.,N.Snapir and H.C.Mel.Biophysical characterization of fowl spermatozoa.I.Preservation of motility and fertilizing capacity under conditions of low temperature and low sperm concentrations[J].Reprod.Fert. 1971,24:153-160.
[153]Polge,C.,Artificiald insemination in fowl.Proc.Soc.Study Fert. 1951, 2:16-18.
[154]Proudfoot,F.G. The influence of oxygen and ther gases on the fertilizing abilityof fowl semen held for several hours at 1O℃.in Cryovac eaclosures[J]. Poultry Sci.1966,45:443-446.
[155]Proudfoot,F.G.,and D.K.R.Stewart. The effect of oxygen and type of container on the retention of the fertilizing capacity of fowl spermatozoa stored in vitro[J].Reprod.Fert.1967,13:251-257.
[156]Schinder,H.,S.Weinstein,E.Moses and I. Gabriel.1955.The effect of various diluents and storage times on the fertilizing capacity of cock semen[J].Poultry Sci.1955,34:1113-1117.
[157]Sexton,T.J.. Effect of centrifugation and repeated washing on the fertilizing capacity of fowl spermatozoa.J.Reprod.Fert.1973,32:101-104.
[158]Sexton,T.J.. Observations on the washing of turkey spermatozoa[C].Proc.15th World Pou1try Congr.1974,391-393.
[159]Sexton,T.J.,and T.A.Fewlass. A new poultry semen extender.II. Effect of the diluent components on the fertility of chicken semen Stored at 5℃[J].Submitted to Poultry Science.1977.
[160]Steel,R.G.D.,and J.H.Torrie. Principles and Procedures of statistics[M],1st Ed.,McGraw-Hill Book Co.,1960,New York.
[161]Van Wambake,F.The effect of oxygenated storage medium on the fertilizing capacity of fowl spermatozoa storage for different periods[C].Proc.6th Cong.Intern.Reprod.Anim.Insem. Artif.Sec.1968,2:1645-1647.
[162]Van Wambake,F. .the effect of two different methods of semen collection on fertility and hatchability results obtainedwith stored fowl semen[C].Proc.5th Europ.Poultry Conf.1976,3:1230-1240.
[163]Weakley,C.E.,and C.S.Shaffner. The feryilizing capacity of diluted chicken semen[J].Poultry Sci.1952,32:650-653.
[164]Shaffner, C.S.,E.W.Henderson,and C. G. Card, Viability of spermatozoa of the chicken undervarious environmental conditions[J].Poultry Sci.1941,20: 259- 265.
[165]Lake,P.E, The history and future of the cryopreservation of avian germ plasm[J].Poultry Sci.1986,65:1-5.
[166]Hammerstedt, R.,and J.K.Graham, Cryopreservation of poultry semen:the enigmaof glycerol[J].Cryobiology.1992,29:26-38.
[167]Bellagamba,F.,S.Cerolini,and L. G.Cavalchini, Cryopreservation for poultry semen: a review[J].World'S Poult.Sci.J.1993,49:158-166.
[168]Sexton,T. J., Viability of frozen chicken semen cooled at various rate to -20℃ in glass ampoules and plastic straws[C].Pages 205-213 in: 16th Congress of the World’s Poultry Science Association.Vo1.2.Rio de Janeiro,Brazil.1978.
[169]Lake,P.E.,0.Ravie,and J.MC Adam, Preservation of fowl semen in liquid nitrogen: application to breeding programmes[J].Poult.Sci.1981,22:71-77.
[170]Lake,P.E.,and O.Ravie, Dimethylacetamide as a cryoprotectant for fowl spermatozoa[J].Poultry Sci.1982,61:1497.(Abstr.)
[171]Lake,P.E.,and 0.Ravie, An exploration of cryoprotective compounds for fowl spermatozoa[J].Poult.Sci.1984,25:145-150.
[172]Schramm,G.P., Deep freezing preservation of cock semen for reproduction of gene reserve population and international sperm exchange[J].Arch.Geflugelkd. 1991, 55(6):258-260.
[173]Seig1eurin,F.,and E .Blesbois, Effects of freezingrate on viability and fertility of frozen-thawed fowl spermatozoa[J].Theriogenology. 1995, 43: 1351-1358.
[174]Tselutin,K.,L. Narubina,T. Maorodina,and B.Tur, Cryopreservation of poultry semen[J].Poult.1995,36:805-811.
[175]fan Voorst,A.,and F.R.Leentra, Fertiiity rate of daily collected and cryopreserved fowl semen[J].Poulty Sci.1995,74:136-140.
[176]Mortimer,R G.,M.E Berndtson, B.W.Pickett,and L.Ben, Fertilizing of frozen bovine spermatozoal packaged in continental straws or ampoules[J]. Dairy Sci. 1976,59:1595-1598.
[177]Sexton,T.J., Preservation of poultry semen.A review.Pages 158-170 in: Animal Reproduction Science.BARC Symposium No.3.H.Hawk,ed.USDA/ARS/BARC,Beltsville, MD.1979.
[178]Duplaix,M.,and T.J.Sexton, Effects of type of freeze straw and thaw temperature on the fertiiizing capacity of frozen chicken semen[J].Poulty Sci.1984,63:75-78.
[179]Latorre,J.R.,G.C.Harris,Jr.,and Z.B.Johnmn, Research note:Influence of storage container for frozen-thawed chicken semen and frequency of insermination on fertility and its duaration[J].Poultry Sci.1988,67:333-335.
[180]Sexton,T.J.,Studies on the fertility of frozen fowl semen[C].pages 1079-1081 in: Proceedings of 8th International Congress on Animal Reproduction, Artificial Insemination, Krakow,Poland.1976.
[181]Lake,P.E.,and J. M.Stewart, Preservation of fowl semen in liquid nitrogen-an improved method[J]. Poultry Sci.1978,19:187-194.
[182]Haije,U., Evaluation and cryopreservation of fowl semen (Gallus domesticus). Doctoral thesis,Rijksuniversiteit te Utrecht,Utrecht,The Netherlands.1990.
[183]Seigneurin,F.,aad E. Blesbois, Some improvements for technique of freezing chicken semen[C].First international Symposium on the Artificial Insemination of Poultry,Beltsville,MD,June 1994,(Abstr.287)
[184] C.W.凯南等. 大学普通化学[M].人民教育出版社. 1980: 151-157
[185] 渊锡藩、张一玲. 动物繁殖学[M].天则出版社. 1993: 51-59、106-125
[186] 华北农业大学等编. 普通化学[M].上海科学技术出版社. 1978:53-102
[187] 牛树理,田宝.渗透压与 pH 对常温保存山羊精液的影响.畜牧与兽医[J].1994,6(26):250
[188] 黄建国.不同渗透压的稀释液对猪精子保存时间的影响.当代畜牧[J].2001,2:27
[189] 张兆旺,张进邦.鸡精液稀释液筛选试验.甘肃农业大学学报.1992,27(4):333～338
[190] 戴有理译.家禽精液稀释液的发展.中国家禽[J].1992(6):34-36
[191] 朱庆等.不同稀释液 2℃─5℃保存鸡精液的研究.四川农业大学学报.1985;3(2):125-133
[192] 曾凡同等.不同稀释液 2℃─5℃保存鸡精液的效果.中国家禽[J].1984(1):31-34
[193] 北京市农科院畜牧兽医研究所养鸡研究室.鸡精液稀释和混合输精的初步研究.家禽[J].1983(1):17-19
[194] 陈元明等.鸡精液稀释保存技术的研究.中国农业科学[J].1987,20(6):52-57
[195] 黄美玉等.不同渗透压稀释液对鸡精子存活率和受精率的影响.1989(2):26-28
[196] 刘建忠.影响鸡精液体外低温保存效果因素的分析.1997,16(2):51-52
[197] E.S.E.哈弗士.农畜繁殖学[M].上海人民出版社,1977:50-51
[198] Sexton T J and T A Fewless. A new poultry semen extender.Effect of the dilluent Components on the fertilizing caoacity of chicken semen sdored at 5℃.
[199] 李尚仁,E F Graham.不同处理对5℃保存的鸡精液的影响.中国农业科学[J].1986(,3):83-89
[200] 张兆旺.鸡精液稀释液设计与应用研究.第二届中国畜牧科技论坛论文集(王健主编)[C]. 2005,中国农业出版社, 199-205
[201] 陈元明,廖纪朝,黄美玉,等.鸡精液稀释液保存技术的研究.中国农业科学[J].1987,20(6):52-57
[202] Wishat.糖对新鲜的和储藏的火鸡精液受精力的影响.甘肃畜牧兽医[J].1987,(2):46
[203] 张坚中, 徐铁铮. 家畜冷冻精液 [M]. 中国农业科技出版社, 1988:27 [204] 苏东顿. 现代家禽人工授精技术.1993: 10
[205] 中国农业科学院主编. 家畜精液稀释和贮存<国外农业科学资料汇编> 第三辑. 农业出版社, 1959: 28-31
[206] 郭年藩译.家畜精液保存技术的研究. 国外畜牧科技[J].1993,1:7-8
[207] 季本林.猪精液稀释液不同用水的研究及应用.中国畜牧杂志[J].1993,(5):40-41
[208] 董金才译.家畜精液冷冻保护液.国外畜牧科技[J] 1986,(6):17-18
[209] 张兆旺. 乌骨鸡体重、冠型、与采精量关系的研究. 甘肃畜牧兽医[J]. 1994 ,6(3):9-11
[210] 张兆旺, 杨进军. 乌骨鸡精液品质测定及人工授精试验.第八届全国动繁殖学术讨会论文集.1995,23
[211] 张兆旺, 李婉平, 史兆国.公鸡体重、采精量、精子活率和密度与受精率关系的研究.第八次全国家禽学术讨论会论文集.1997,19-20
[212] 陈元明,廖纪朝,黄美玉,等.鸡精液稀释液保存技术的研究.中国农业科学[J].1987,20(6):52-57
[213] 郭年藩译. 家禽精液稀释液的发展.国外畜牧科技[J].1992,19(6):8-11
[2]Froman,D.P., and D.J.McLean, Objective measurement of sperm motility based upon sperm penetration of Accudenz[J].Poultry Sci.1996,75:776-784
[3]Proman,D.P.,A.J.Feltmann,and D.J.McLean, Increased fecundity resulting from semen donor selection based upon in vitro sperm motility. Poultry Sci.1997, 76:73-77
[4]Allen,C.J.,and L.R. champion, Competitive fertilization in the fowl[J].Poultry Sci.1995,34:1332-1342
[5]Cooper,D.M.,and J.G.Rowell, Relations between fertility,embryonic survival and some semen characteristics in the chicken[J].Poultry Sci.1958.37:699-707
[6]Kamar,G.A.R., The influence of semen characteristics on hatching results of chicken eggs.Poultry Sci.1960,39:199-192
[7]McDaniel,G.R.,and J.V.Craig, Predicting male fertilizing capacity in high and low fertility strains of chickens[J].Poultry Sci.1962,41:866-869
[8]Wishart,G.J.,and F.H.Palmer, Correlation of the fertilizing ability of semen from individual male fowls with sperm motility and ATP cointent[J].Poultry Sci. 1986,27:97-102
[9]Ashizawa,K.,and R.Sano, Effects of temperature on the immobilization and the initiation of moyility of spermatozoa in the male reproductive tract of the domestic fowl,Gallus domesticus[J].Comp.Biochem.Physiol.1990,96A:297-301
[10]Amann,R.P.,and R.H.Hammerstedt, In vitro evaluation of sperm quality:An opinion[J].Androl.1993,14:397-406
[11]Wilson,H.R.,N.P.Piesco,E.R.Miller,and W.G.Nesbeth, Prediction of the fertility potential of broiler breeder males[J].World’s Poult.Sci. J. 1979, 35:95-118
[12]Bakst,M.R.,and H.C. cecil, Techniques for semen evaluation,semen storage,and fertility determination[J].Poultry Science Association, Inc., Savoy,IL. 1997.
[13]Bilgili,S.f.,and J.A.Renden,Fluorometric determination of avain sperm viability and concentration[J].Poultry Sci.1984,63:2275-2277
[14]McDaniel,C.D.,J.L.Hannah,H.M.Parker,C.D.Schultz,and C.D.Zumwalt, The relati- onship of the sperm motility index with rooster sperm quality and fertilizing ability[J].Poultry Sci.1997,76(Suppl.1):115.(Abstr.)
[15]Wishart,G.J.,and Y.I.Wilson Sperm motility and metabolism IV.Spermmotility analysis using the “Sperm quality analyzer”.Pages54-55 in: Techniques for semen evaluation,semen storage,and fertility determination. M.R.Bakst and H.C.Cecil, ed.Poultry Science Association,Savoy,IL. 1997.
[16]Bartoov,B.,J.Ben-Barak,A.Mayevsky,M.Sneider,L.Yogev,and A.Lightman, Sperm motility index :a newparameter for human sperm evaluation[J]. Fertil. 1991, 56:108-112
[17]Johnston,R.C.,G.N.Clarke,D.Y.Liu,and H.W.G.Bake, Assessment of the sperm quality analyzer[J].Fertil.Steril.1995,63:1071-1076
[18]Matilsky,M.,M.BEN-Ami,V.Eyali,Y.Geslevieh,Y.BEN-Barak,and E.Shalev, Correlation between sperm motility index as measured by the sperm quality analyzer and the outcome of intrauterine inseminations.Human Reprod. 1993, 8(Suppl.1): 98.(Abstr.)
[19]McDaniel,C.D.,J.L.Hannah,H.M.Parker,T.W.Smith,C.D.Schultz,and C.D.Zumwalt, Use of a sperm analyzer for evaluating broiler breeder males.1.Effects of altering sperm quality and quantity on the sperm motility index[J].Poultry Sci. 1998,77: 888-893
[20]Guyton,A.C.,Page 890 in:Textbook of Medical physiology.8th ed.W.B.Saunders Co.,Philadelphia,PA.1991.
[21]Clarke,R.N.,T.J.Sexton,and M.A.Ottinger, Effects of holding temperature and storage time on respiratory rate,motility,and fertility of chicken and tuekey semen[J].Poultry Sci.1982.61:1912-1917
[22]Sexton , T.J. , Preservation of poultry semen.A review.Pages 159-170 in: Beltsville Symposia in Agricultural Research. 3.Animal Reproduction Allanheld,Osmun and Co.,Montclair,NJ.1979.
[23]Wishart,G.J., The effect of anoxia on fowl spermatozoa:Recovery of fertilizingability,motility and cellular concentrations of potassium and ATP[J].Gamete Res. 1984,10:83-89
[24]Vantman,D.,S.M.Banks,G.Koukoulis,L.Dennison,and R.J.Sherins, Assessment of sperm motion characteristics from fertile and infertile men using a fully automated computer-assissted semen analyzer[J].Fertil.1989,51:156-161
[25]Froman,D.P.,and D.J.McLean, Objective measurement of sperm motility based upon sperm penetration of Accudenz[J].Poultry Sci.1996,75:776-784
[26]Bakst,M.R.,G.Wishart,and J.P.Brillard, Oviducal sperm selection, transport, and storage in poultry[J].Poult.Sci.Rev.1994,5:117-143.
[27]Thomson,M.F., and G.j.Wishart, Elucidation of the mechanism responsible for the temperature-dependent reversible inactivation of the motility of fowl spermatozoa.Br[J].Poult.Sci.1988,30:687-692.
[28]Ashizawa,K.,S. Maeda,and K.Okauchi, The mechanisms for reversible immobilize- ation of fowl spermatozoa at body temperature[J]. Reprod. Fertil.1989,86: 271-276.
[29]Ashizawa,K.,A.Hashiguchi,and Y.Tsuzuki,Intracellular free Ga2+ concentration in fowl spermatozoa and its relationship to motility and respiration in spermatozoa.[J].Reprod.Fertil.1992,96:395-405.
[30]Thoumon,M.F.,and G.J.Wishart, Temperature-mediated regulation of calcium flux and moutility in fowl spem[J]. Reprod.Fertil.1991,93:385-391.
[31] Ashizawa,K.,H.Tomonaga,and Y. Tsuzuki, Regulation of flagellar motility of fowl spermatozoa: evidence for the involvement of intracellular Ga2+ and calmodulin [J].Reprod.Fertil.1994,101:265-272.
[32]Ashizawa,K.,and G. J. Wishart, Resolution of the sperm motility- stimulating principle of fowl seminal plasma in Ga2+ and an unidentified low molecular Weight factor[J].Reprod.Fertil.1987,81:495-499.
[33] Bakst M.R., and H.C.Cecil, Effect of bovine serum albumin on motility and fecundity of turkey spermatozoa before and after storage. J. Reprod.Fertil. 1992,94:287-293.
[34]Mohau, J.,M. Saini, and P. Joshi, Isolation of a spermatozoal motility inhib1ting factor from chicken seminal plasm with antibacterial property[J].Biochem. Biophys.Acta.1995,1245:407-413.
[35]Lake,P.E., The male in reproduction. Pages 381-405 in: Physiology and Biochemi- stry of the Domestic Fowl.Vo1.5.B.M.Freeman,ed. Academic Press, London, UK.1984.
[36]Lesslley,B.A.,and K. I. Brown,.Purification and properties of a proteinase inhibitor from chicken seminal plasma[J].Biol.Reprod.1978,19:223-234.
[37]Freeman,B. M., Appendix:Biochemical and physiological data. Pages 407-424 in: Physiology and Biochemistry of the Domestic Fowl.Vol.5.B.M.Freeman and D.J.Bell, ed. Academic Press, London, UK.1984.
[38]Froman,D.P., and K. A. Thursam, Desialylation of the rooster sperm's glycocalyx decreases sperm sequestration following intravaginal insemination of the hen. Biol.Reprod.1994,50:1094-1099.
[39]Wishart,G.J.,and K.Ashizawa, Regulation of the motility of fowl spermatozoa by calcium and cAMP .J.Reprod.Fertil.1987,80:607-611.
[40]Goldberg,E.,and C.Norman, The metabolism of ejaculated spermatozoa from the fowl[J].Cell Comp.Physiol.1961,58:175-180.
[41]Sexton , T.J. , Oxidative and glycolytic activity of chicken and turkey spermatozoa[J]. Comp. Biochem.Physiol.1974,488:59-65.
[42]Wishart,G.J., Maintenance of ATP concentrations in and of fertilizing ability of fowl and turkey spermatozoa in vitro[J]. Reprod.Fertil.1982,66:457-462.
[43]Howarth,B.,Jr., Oxidation of [U-14C] palmitic acid by cock spermatozoa[J]. Poultry Sci.1978,57:1751-1754.
[44]Howarth,B,Jr., The phospholipid profile of cock spermatozoa before and after in vitro incubation for twenty-four hours at 41℃[J]. Poultry Sci.1981,60: 1516-1519.
[45]N.Fujihara [J]World`s Poultry Science Journal 1992, Vol.48 No.1,39-55
[46]Bogdonoff,P.D.,and C.S.Shaffner,The effect of pH on the in vitro survival, metabolic activity,and fertilizing capacity of chicken semen[J].Poultry Sci., 1954,33:665-669
[47]Dunnett, C.W..A multiple compartson procedure for comparing several treatments with acontrol[J].Amer.Statist-Assoc.1955,50:1096-1121.
[48]Fewlass,T.A.,T.J.Sexton and C.S.Shaffner. Effect of various levels of egg yolk,milk,seminalplasma or blood serum on the respiration andreproductive efadeney of chicken spermatozoa[J]. Poultry Sci.1975,54:346-349.
[49]Flipse,R.J.,S.Patton and J.O.AImquist, Dilutors for bovine semen.III.Effect of lactenin and lactoperoxidase upon spermatozoa livability[J].Dairy Sci.1954, 37:1205-1211.
[50]Hobbs,T.D.,and G.C. Harris,Effect of freezing point depression and CO2 on motility and fertility of chicken spermatozoa stored in carbondioxide extenders[J].Poultry Sci.1963,42:388-393.
[51]Lake,P.E..Studies on the dilution and storage of fowl semen[J]. Reprod. Fem. 1960,1:30-35.
[52]Lake,P.E.,and W.M.Mcindoe,The glutamic acid and creatine content of cock seminal plasma[J].Biochem.1959,J.71:303-306.
[53]Ogasawara,F.X.,and R.A.Ernst.Recent developments in the artificial insemin- ation of turkeys[J].Feedstuffs.1975,47:23-35.
[54]Polge,C.,Artificial insemination in the fowl[C].Proc.Soc.Study Fert.1950, 2:16-22.
[55]Schindler,H.,S.Weinstein,E.Moses and I Gabriel.The effect of cock semen. Poultry Sci.1955,34:1113-1117.
[56]Sexton,T.J.Oxidative glycolytic acdvity of chicken and turkey spermatozoa[J]. Comp.Biochem.Physiol.1974,48B:59-65.
[57]Sexton,T.J..Studies on the dilution of turkey semen[J].Poultry Sci.1976, 17:179-184.
[58]Shannon,P..Presence of a heat labile toxic protein in bovine seminal plasma[J]. Dairy Sci.1965,48:1362-1366.
[59]Smith,A.U.,1949.The control of bacterial growth in fowl semen[J].Ag.Sci.1949, 39:194-200.
[60]Van Tienhoven,A.,R.G.D.Steel and S.A.Duchaine.Diluents for turkey semen[J]. Poultry Sci.1958,37:47-53.
[61]Van Wambake,F..The storage of fowl spermatozoa.I.Preliminary results with diluents[J]. Reprod.Fert.1967,13:571-575.
[62]Wales , R.G. , and I. G.White.The viability of fowl spermatozoa in dilute suspension[J].AustralianJ. Biol.Sci.1961,14:637-645.
[63]Weakly,C.E.,and C.S.Shaffner.1952.The fertilizing capacity of diluted chicken semen[J].Poultry Sci.1952,32:650-653.
[64]White,I.G..The effect of potassium on the washing and dilution of mammalian spermatozoa.Australian [J].Exp.Biol.1953,31:193-200.
[65]Wilcox,F.H..The effect of different hydrogenion concentrations during storage and at insemination and of added magnesium and potasium on the fertilizing ability of chicken semen[J].Poultry Sci.1959,38:1159 一 1162.
[66]Wilcox,F.H. Effect on fertility of temperature handling methods,Lake's solution,and the addition of egg white,egg yolk and sugars to the diluent used in Storing chicken semen[J].pou1try Sci.1960,39:459-467.
[67]Wilcox,F.H.,and M.S.Shorb.The effect of antibiotics on bacteria in semen and on motility and fertilizing ability of chicken spermatozoa.Amer.J.Vet.Res. 1958,19:945-949.
[68]Yamane,J.,s.Tsukanaga and T.Takahashi.A basic principle of make-up of the dilutor for fowl semen[J].Zootech.Vet.1692,17:523-527.
[69]Sexton.T.J,T.A.Fewlass.A new poultry extender 2.effect of the diluent components on the fertilizing capacity of chicken semen stored at 5℃[J].Poultry Sci.1977,56:277-284.
[70]Sexton,T.J. Comparison of commercial diluents for holding turkey semen 24 hours at 5C[J].Poultry Sci .1988a,67:131-134.
[71]Lake,P.E. Recent progress in poultry reproduction[J].World’s Poult.Sci.J. 1989,45:53-59
[72]Sexton,T.J. Research note:Influence of damaged spermatozoa on the fwertility of turkey of turkey semen stored for 24 hours at 5C[J].Poultry Sci.1988b, 67: 1483-1485.
[73]Wishart, G.J., Physiological changes in fowl and turkey spermatozoa during in vitro storage[J]. Br.poult .sci.1989,30:443-454.
[74]Bakst.M.R.H.C.Cecil.snd T.J.Sexton, Modeficatiion of the ethidium bromide exclusion procedure for evaluation of turkey sperm[J].Poultry Sci.1991 ,70:336-370.
[75]Bakst M.R.G. Wishart.and J.P.Brillard . Oviductal sperm selection transport and storage in poultry[J]. Poult.Sci.Rev.1994,5:117-143,
[76]Thurston ,R,J., Storage of poultry semen above freezing for 24-48 hours .pages 107-122 in :proceedings of the First international symposium on the artificial Insemination of poultry .M.R.Bakst and G.J.wishart , ed poultry science Association ,savoy IL.1995.
[77]Wishart ,G.J., The effect of continuous aeration on the fertility of fowl and turkey semen stored above 0C[J].poult.sci.1981,22:445-450
[78]Sexton,T.J.and A.F.Giesen, Beltsville Poultry Semen Extender.6.Hloding turkey semen for six hours at 15C[J].Poultry Sci.1982,61:1202-1208.
[79]Jones,R,and T.Mann, Toxicity of sxogenous fattu acid peroxides towards spermatozoa[J].Reprod .Fertil.1973,50:255-260
[80]Jones,R.,and T.Mann, Toxicity of exogenous fatty acid peroxides towards spermatozoa[J].Reprod.Fertil.1977,50:255-260.
[81]Fujihara,N.and B.Howarth,Jr. Lipid peroxidation in fowl spermatozoa[J].Poultry Sci.1978,57:1766-1768.
[82]Fujihara,N.and O.Koga, Prevention of the production of lipid peroxide in rooster spermatozoa[J] .Anim.Reprod Sci.1984,7:385-390
[83]Wishart ,G.J., Effects of lipid peroxide formation in fowl semen on sperm motility .ATP content and fertilizing ability[J].Reprod . Fertil .71:113-118.
[84]Ravie,O,and P.E.Lake, The phospholipids –bound fatty acids of fowl and turkey spermatozoa[J]. Anim.Reprod.Sci.1984,9:189-192.
[85]Alvarez.J.G.and B.T.Storey, Role of superoxide dismutase in protecting rabbit spermatozoa from O2 toxicity due to lipid perpxodatoon[J]. Biol.Reprod. 1983,28:1129-1136.
[86]Alvarez.J.G.C.Touchstone,L.Blasco,and B.Storey, Spontaneous lipid peroxide- atong and production of hydrogen peroxide and superoxide in human soermatozoa superoxide dismutase as major enzyme p[rotectant against oxygen toxicity[J]. Androl.1987,8:338-348.
[87]Aitlen,R.J.S.Clarkson. Significance of reactive oxygen species and antioxidantsin defining the efficacy of spermatozoa preparation techniques[J]. Androl. 1988, 9:367-376.
[88]Msxwell,W.M.C.and T. Stojanoc, Liquid storage of ram semen in the absence or presence of some antioxidants[J]. Report .Fertil.Dev.1996,8:1013-10120.
[89]Hammerstedt,R.H. Maintenance of bioenergetis balance in sperm and prevention of lipid peroxidation:A review of the effect on design of storage preservation systems[J].Reprod Fertil.Dev.1993,5:657-690.
[90]Blesboid E.I.Grasseau,and J.C.Blum, Effects of vitamin E on fowl semenstorageat 4C[J].Theriogenology 1993,39:771-779.
[91]Sural.P.F. Relations between vitamin E concentration in poultry spermatozoa and some semen biochemical and physiological characteristic .page 171-173 in : program of the 8th .International Symposia on Current problem in avian Genetics, Slovak academy science ,smolenice,slovakia.1989.
[92]Donoghue,A.M.D.L.Gatner,D.J.Donoghue.and L.A.Johson, Viability assessment of turkey sperm using fouo9rescent staining and golw cytometry[J].Poultry Sci. 1995,74:1191-1120.
[93]Donoghue A.M.D.L.Gatner D.J.Donoghue ,and L.Ajohnson, Assessment of the membrane integrity of fresh and stored turkey spermatozoa using a combination of hypoosmotis stress,fluorescent staining and flow cytometry[J]. Theriogenology 1996,46:153-163.
[94]Burton .G.W.and K.U.Ingold, Mechanisms of antioxidant action :prevention and chain –breaking antioxidants.pages 29-48 in:CRCHandbook of Free Radicals and Antiocidants in Biomedicine. Vol II.A.Maquel.T.Quintanilha . and.H.Weber, ed.Boca Taton.FL.1988.
[95]Fromen.D.P. and R.J.Thurston, Chicken and turkey spermatozoal superoxide dismutase:A comparative study[J]. Biol.Repord.1981,24:193-200.
[96]Cecil.H.C.and M.R.Bakst, Invitro lipid peroxidation of turkey spermatozoa[J]. Poultry Sci.1993,72:1370-1378.
[97]Smith, A. V., and C. Polge., Survival of spermatozoa at low temperatures[J]. Nature.1950,166-668.
[98]Ax, R. L., and J. R. Lodge, Rooster spermatozoa motility, forward progressionand fertility after storage of 25, 5, or –196C in various extenders[J]. Cryobiology. 1975,12:93-97.
[99]Polge, C., Functional survival of fowl spermatozoa after freezing at –79C[J]. Nature. 1951,167:949-950.
[100]Clark, C. E., and C.S. shaffner, The fertilizing capacity of frozen chicken sperm and the influence of related in vitro processes. Poultry Sci.1960,39: 1213-1222.
[101]Graham, E. F., and M. M. Pace, Some biochemical changes in spermatozoa due to freezing[J]. Cryobiology.1967,4:75-84.
[102]Brown, K. I., B.G. Crabarm, and M.M.Pace, Some factors affecting loss of intracellular enzymes from spermatozoa[J]. Cryobiology.1971,8:220-224.
[103]Sexton, T. J ., Comparison of various cryoprotective agents on washed chicken spermatozoa. 4. Metabolism and release of glutamic oxalacetic transaminase[J]. Poultry Sci.1974,53:284-287.
[104]Pace, M.M., and E.F. Graham, The release of glutamic oxaloacetic transaminase from bovine spermatozoa as a test method for assessing semen quality and fertility[J].Biol.Reprod.1970,3:140-146.
[105]Westfall, F.D., and G.C. Harris, Jr., The ability of cryopreservatives to prevent motility loss and freeze-thaw damage to the acrosome of chicken spermatozoa[J].Cryobiology.1975,12:89-92.
[106]Thurston, R. J., and G. C. Harris, Jr., Calcium-45 uptake as a measurement of freeze-thaw injury to chicken spermatozoa in the presence or absence of cryopreservatives[J].Cryobiology 1970,7:100-106.
[107]Allen, T.E., and L.W, The fertility of fowl spermatozoa in glycerol diluents after intrauterine insemination[J]. Poultry Sci.1995,34: 1167—1169.
[108]Westfall, F. D., and B, Howarth, J r. Duration of the antifertility effectof glycerol in the chicken vagna[J].Poultry Sci.1976,56:924-925.
[109]Marquez, B.J., and F. X., Ogasawara, Effects of glycerol on turkey sperm cell viability and fertilizing capacity[J]. Poultry Sci.1975, 54:1788(Abstr.)
[110] Westfall.F.D and Birkett howarth,JR. The effect of glycerol and dilution on the release of glutamic oxaloacetic transaminase from chicken spermatozoa[J].Poultry Sci.1978,57:1037-1041.
[111]Christensen, V.L., Diluents dilution and storage of turkey semen for six hours. Pages 90-106 in: First International Symposium on the Artificial insemination of poultry[R]. M.R.Bakst and G.J.Wishart, ed. Poultry Science Association, Savoy, IL.1995.
[112]Lake, P.E., Historical perspective of artificial insemination tech1nology. Pages 1-20 in: First Internationaal Symposium on the Artificial inseminaaon of Pourtry[C]. M.R.Bakst and G.J.Wishart,ed., Poultry Science Association, Savoy, IL.1995.
[113]Thurston,R.J., Storage of poultry semen above freezing for 24 to 48 hours[C]. Pages 107-122 in: First International Symposium on the ArtinciaI Insemination of Poultry. M.R Bakst and G.J.Wishad, ed. Poultry Science Association, Savoy, IL.1995.
[114]Leopold,A.C, Membranes,Metabolism and dry organism[M]. Cornell University Press, Ithaca, NY.1986.
[115]Ried,J.L.,and M.K.Walker-Simmons, Group 3 late embryogenesis abundant proteins in desiccation-tolerance seedings of wheat (Triticum aestivum L) [J]. Plant Physiol.1993,102:125-131.
[116]Churchill, T.A., and K.B.Storey, Dehydration tolerance in the wood frogs: a new perspective on development of amphibian freeze tolerance[J].Am.J.Phys. 1993,265:1324-1332.
[117]Kemode, A.R., Regulatory mechanisms in the transition from seed development to germination: Interractions between the embryo and he seed environment. Pages 273-332 in:Seed Development and Gemination[J].Kigel and G.Galili, ed., Marcel Dekker,Inc.,New York, NY.1995.
[118]Dure,L.,III,M.Crouch,J.Harada,T.H.D.Ho,Mundy,R.Quatrano,T.Thomas,and Z.R. Sung, Common amino acid sequences domains among the LEA proteins of higher plants-Plant[J]. Mol.Biol.1989,12:475-486.
[119]Jacobsen, J.V., and D.C Shaw, Heat-stable proteins and abscicic acid action in barley aleurone cells[J]. Plant Phys.1989,91:1520-1526.
[120]Ried,J.L.,and M.K. Walker-Simmons, Synthesis of abscisic acid-responsive, heat-stable proteins in embryonic axes of dormant wheat grain[J]. Plant Physiol.1990,93:6624
[121]Lin, W.W.Cuo, E.Everson, and M.F.Thomashow, Cold acclimation in Arabidopis and wheat. Aresponse associated with expression of related genesencoding “boiling-stable” polypeptides[J]. Plant Physiol.1990, 94:1078-1083.
[122]Walters,C.J., L.Reid, and M.K.Walker-Simmons, Heat-soluble proteins extracted from wheat embryos have tightly bound sugars and unusual hydration properties[J]. Seed Sci.Res.1997, 7:125-134.
[123] Lin,C.,and M.F.Thomashow, A cold-regulated Arabidopsis gene encodes a polypeptide having potent cryoprotectant activity[J]. Biochem. Biophys. Commun. 1992, 183:1103-1108.
[124]Luo,J., and B.M.Reed, Abscisic acid-responsive protein, bovine semen albumin, and praline pretreatments improverecovery of in vitro currant shoot-tip meristems and callus cryopreserved by vitrification[J]. Cryobiology 1997, 34:240-250.
[125]Donoghue,A.M.,M.K.Walker-Simmons, The influence of wheat dehydration-induced proteins on the function of turkey spermatozoa after twenty-four-hour in vitro storage[J]. Poultry Sci.1999, 78:235-241.
[126]Sexton,T.J., Comparison of commercial diluents for holding turkey semen 24 hours at 5℃[J]. Poultry Sci.1988,67:131-134.
[127]Bakst, M.R., and H.C.Cecil, Effect of bovine serum albumin on motility and fecundity of turkey spermatozoa before and after storage [J]. Reprod. Fertil. 1992, 94:287-293.
[128]Artus,N.N.,M.Uemura, P.L. Steponkus, S.J. Gilmour, C. Lin, and M.F.Thomashow, Constitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance[J].Proc.Natl. Acad.Sci.1996, 93:13404-13409.
[129]Wishart, G.J., Maintenance of ATP concentration in and of fertilizing ability of fowl and turkey sperm in vitro[J].J.Reprod.Fertil.1982,66:457-462.
[130]Irvine,D.S.,and R.J.Aitken, The value of adenosine triphosphate (ATP)measurements in assessing the fertilizability of human spermatozoa. Fertil- Steril.1985,44:806-813.
[131]Soderquist, L., H.Rodriguez-Martinez, and L. Janson, Post-thaw motility, ATP content and cytochrome C oxidase activity of AI bull spermatozoa in relation to fertility[J].Zentrabl.Vet.1991,38:165-174.
[132]Froman, D.P.,and D.J. McLean, Objective measurement of sperm motility based upon sperm penetration of Accudenz. Poultry Sci.1996,75:776-784.
[133]Donoghue,A.M.,D.R.Holsberger, D.P.Evenson, and D.P.Froman, Semen donor selection by in vitro sperm mobility increases fertility and sperm storage in the turkey hen[J].Androl.1998,19:295-301.
[134]Baks, M.R., G.Wishart, and J.P.Brillard, Oviductal sperm selection, transport and storage in poultry.Poult.Sci.Rev.1994,5:117-143.
[135]Brillard , J.P. , nd M.R. Bakst , Quantification of spermatozoa in the spermatozoa-storage tubules of turkey hens and the relation to spermatozoa numbers in the perivitelline layer of eggs[J]. Biol.Reprod.1990,43:271-275.
[136]Donogue, A.M., M.R.Bakst, D.R.Holsberger, and D.J.Donoghue, Effect of semen storage on sperm numbers in the perivitelline layer of laid turkey eggs[J].Reprod.Fetil.1995a,105:221-225.
[137]Donoghue, A.M.,D.L Garner, D.j.Donoghue, and L.A.Johnson, Viability assessment of turkey spermusing fluorescent staining and flow cytometry[J]. Poultry Sci.1995b, 74:1191-1200.
[138]Anderson, M.M., and C .P.Merilan, Dehydration studies on bovine spermatozoa. Pages 1-35in: Research Bulletin, University of Missouri Agricultural Experiment Station,Columbia,M0. 1960.
[139]Mazur,P., Freezing of living cells: Mechanisms and implications[J].Physiol. 1984,247:C125-142.
[140]Hammerstedt, R.H., J.K.Graham, and J.P.Nolan, Cryopreservation of mammalian sperm: What we ask them to survive[J].Andro1.1990,11:73-87.
[141]Dunnett,C.W..A Multiple comparison procedure for comparing several treatments with a control[J].Amer.Statist.Assoc.1955,50:1096-1121.
[142]Fewlass,T.A.,T.J.Sexton and C.S.Shaffner.Effect of various levels of egg yolk,milk,seminal plasma or blood serum on the respiration and reproductive efaciency of chicken spermatozoa[J].Pouley Sci.1975,54:346-349.
[143]Harris,G.C.,and T.D.Hobbs.The effects of fluid to gas ratio,dilution rate,and CO2 level on the fertilizing capacity of chicken spermatozoa stored in carbon dioxide extenders[J].Poultry Sci.1964,43:529-534.
[144]Harris,G.C.,and T.D.Hobbs.1968.Effects of freezing point depression and fluid to gas ratio on fertility on fowl spermatozoa stored in C02 extenders[J]. Reprod.Fert.1968,16:389-394.
[145]Sexton.T.J..A new poultry semen extender 3.Effect of storage conditions on the fertilizing capacity of chicken semen stored at 5℃[J].Poultry Sci.1977, 55: 285-289.
[146]Hunsaker,W.G.,and J.R.Aitken.The effect of visible light on the fertilizing capacity of fowl spermatozoa[J].Poultry Sci.1960,39:865-867.
[147]Sexton.T.J.A neu poultry semen extender 3.effect of storage conditions on the fertilizing capacity of chicken semen stored at 5℃[J]. Poultry Sci.1977, 55:285-289.
[148]Lake,P.E.. A retarding factor in the problem of fowl semen storage. proc.3rd Int.Congr.Anim.Reprod.Sec.1956,3:104-106.
[149]Lake,P.E.. Fowl semen as collected by the massage method.J. Ag.Sci.1958, 49:120-126.
[150]Norman,C.,and E.Goldberg. Effect of light on motility,life-sperm and respiration of bovine spepermatozoa[J].Science.1959,130:624-625.
[151]Norman,C.,E.Goldberg and I.D.Porterfield. The effect of visible radiation ofthe functional life-span of mammalian and avian spermatozoa Exp[J].Cell Res.1962,28:69-72.
[152]Pistenma,D.A.,N.Snapir and H.C.Mel.Biophysical characterization of fowl spermatozoa.I.Preservation of motility and fertilizing capacity under conditions of low temperature and low sperm concentrations[J].Reprod.Fert. 1971,24:153-160.
[153]Polge,C.,Artificiald insemination in fowl.Proc.Soc.Study Fert. 1951, 2:16-18.
[154]Proudfoot,F.G. The influence of oxygen and ther gases on the fertilizing abilityof fowl semen held for several hours at 1O℃.in Cryovac eaclosures[J]. Poultry Sci.1966,45:443-446.
[155]Proudfoot,F.G.,and D.K.R.Stewart. The effect of oxygen and type of container on the retention of the fertilizing capacity of fowl spermatozoa stored in vitro[J].Reprod.Fert.1967,13:251-257.
[156]Schinder,H.,S.Weinstein,E.Moses and I. Gabriel.1955.The effect of various diluents and storage times on the fertilizing capacity of cock semen[J].Poultry Sci.1955,34:1113-1117.
[157]Sexton,T.J.. Effect of centrifugation and repeated washing on the fertilizing capacity of fowl spermatozoa.J.Reprod.Fert.1973,32:101-104.
[158]Sexton,T.J.. Observations on the washing of turkey spermatozoa[C].Proc.15th World Pou1try Congr.1974,391-393.
[159]Sexton,T.J.,and T.A.Fewlass. A new poultry semen extender.II. Effect of the diluent components on the fertility of chicken semen Stored at 5℃[J].Submitted to Poultry Science.1977.
[160]Steel,R.G.D.,and J.H.Torrie. Principles and Procedures of statistics[M],1st Ed.,McGraw-Hill Book Co.,1960,New York.
[161]Van Wambake,F.The effect of oxygenated storage medium on the fertilizing capacity of fowl spermatozoa storage for different periods[C].Proc.6th Cong.Intern.Reprod.Anim.Insem. Artif.Sec.1968,2:1645-1647.
[162]Van Wambake,F. .the effect of two different methods of semen collection on fertility and hatchability results obtainedwith stored fowl semen[C].Proc.5th Europ.Poultry Conf.1976,3:1230-1240.
[163]Weakley,C.E.,and C.S.Shaffner. The feryilizing capacity of diluted chicken semen[J].Poultry Sci.1952,32:650-653.
[164]Shaffner, C.S.,E.W.Henderson,and C. G. Card, Viability of spermatozoa of the chicken undervarious environmental conditions[J].Poultry Sci.1941,20: 259- 265.
[165]Lake,P.E, The history and future of the cryopreservation of avian germ plasm[J].Poultry Sci.1986,65:1-5.
[166]Hammerstedt, R.,and J.K.Graham, Cryopreservation of poultry semen:the enigmaof glycerol[J].Cryobiology.1992,29:26-38.
[167]Bellagamba,F.,S.Cerolini,and L. G.Cavalchini, Cryopreservation for poultry semen: a review[J].World'S Poult.Sci.J.1993,49:158-166.
[168]Sexton,T. J., Viability of frozen chicken semen cooled at various rate to -20℃ in glass ampoules and plastic straws[C].Pages 205-213 in: 16th Congress of the World’s Poultry Science Association.Vo1.2.Rio de Janeiro,Brazil.1978.
[169]Lake,P.E.,0.Ravie,and J.MC Adam, Preservation of fowl semen in liquid nitrogen: application to breeding programmes[J].Poult.Sci.1981,22:71-77.
[170]Lake,P.E.,and O.Ravie, Dimethylacetamide as a cryoprotectant for fowl spermatozoa[J].Poultry Sci.1982,61:1497.(Abstr.)
[171]Lake,P.E.,and 0.Ravie, An exploration of cryoprotective compounds for fowl spermatozoa[J].Poult.Sci.1984,25:145-150.
[172]Schramm,G.P., Deep freezing preservation of cock semen for reproduction of gene reserve population and international sperm exchange[J].Arch.Geflugelkd. 1991, 55(6):258-260.
[173]Seig1eurin,F.,and E .Blesbois, Effects of freezingrate on viability and fertility of frozen-thawed fowl spermatozoa[J].Theriogenology. 1995, 43: 1351-1358.
[174]Tselutin,K.,L. Narubina,T. Maorodina,and B.Tur, Cryopreservation of poultry semen[J].Poult.1995,36:805-811.
[175]fan Voorst,A.,and F.R.Leentra, Fertiiity rate of daily collected and cryopreserved fowl semen[J].Poulty Sci.1995,74:136-140.
[176]Mortimer,R G.,M.E Berndtson, B.W.Pickett,and L.Ben, Fertilizing of frozen bovine spermatozoal packaged in continental straws or ampoules[J]. Dairy Sci. 1976,59:1595-1598.
[177]Sexton,T.J., Preservation of poultry semen.A review.Pages 158-170 in: Animal Reproduction Science.BARC Symposium No.3.H.Hawk,ed.USDA/ARS/BARC,Beltsville, MD.1979.
[178]Duplaix,M.,and T.J.Sexton, Effects of type of freeze straw and thaw temperature on the fertiiizing capacity of frozen chicken semen[J].Poulty Sci.1984,63:75-78.
[179]Latorre,J.R.,G.C.Harris,Jr.,and Z.B.Johnmn, Research note:Influence of storage container for frozen-thawed chicken semen and frequency of insermination on fertility and its duaration[J].Poultry Sci.1988,67:333-335.
[180]Sexton,T.J.,Studies on the fertility of frozen fowl semen[C].pages 1079-1081 in: Proceedings of 8th International Congress on Animal Reproduction, Artificial Insemination, Krakow,Poland.1976.
[181]Lake,P.E.,and J. M.Stewart, Preservation of fowl semen in liquid nitrogen-an improved method[J]. Poultry Sci.1978,19:187-194.
[182]Haije,U., Evaluation and cryopreservation of fowl semen (Gallus domesticus). Doctoral thesis,Rijksuniversiteit te Utrecht,Utrecht,The Netherlands.1990.
[183]Seigneurin,F.,aad E. Blesbois, Some improvements for technique of freezing chicken semen[C].First international Symposium on the Artificial Insemination of Poultry,Beltsville,MD,June 1994,(Abstr.287)
[184] C.W.凯南等. 大学普通化学[M].人民教育出版社. 1980: 151-157
[185] 渊锡藩、张一玲. 动物繁殖学[M].天则出版社. 1993: 51-59、106-125
[186] 华北农业大学等编. 普通化学[M].上海科学技术出版社. 1978:53-102
[187] 牛树理,田宝.渗透压与 pH 对常温保存山羊精液的影响.畜牧与兽医[J].1994,6(26):250
[188] 黄建国.不同渗透压的稀释液对猪精子保存时间的影响.当代畜牧[J].2001,2:27
[189] 张兆旺,张进邦.鸡精液稀释液筛选试验.甘肃农业大学学报.1992,27(4):333～338
[190] 戴有理译.家禽精液稀释液的发展.中国家禽[J].1992(6):34-36
[191] 朱庆等.不同稀释液 2℃─5℃保存鸡精液的研究.四川农业大学学报.1985;3(2):125-133
[192] 曾凡同等.不同稀释液 2℃─5℃保存鸡精液的效果.中国家禽[J].1984(1):31-34
[193] 北京市农科院畜牧兽医研究所养鸡研究室.鸡精液稀释和混合输精的初步研究.家禽[J].1983(1):17-19
[194] 陈元明等.鸡精液稀释保存技术的研究.中国农业科学[J].1987,20(6):52-57
[195] 黄美玉等.不同渗透压稀释液对鸡精子存活率和受精率的影响.1989(2):26-28
[196] 刘建忠.影响鸡精液体外低温保存效果因素的分析.1997,16(2):51-52
[197] E.S.E.哈弗士.农畜繁殖学[M].上海人民出版社,1977:50-51
[198] Sexton T J and T A Fewless. A new poultry semen extender.Effect of the dilluent Components on the fertilizing caoacity of chicken semen sdored at 5℃.
[199] 李尚仁,E F Graham.不同处理对5℃保存的鸡精液的影响.中国农业科学[J].1986(,3):83-89
[200] 张兆旺.鸡精液稀释液设计与应用研究.第二届中国畜牧科技论坛论文集(王健主编)[C]. 2005,中国农业出版社, 199-205
[201] 陈元明,廖纪朝,黄美玉,等.鸡精液稀释液保存技术的研究.中国农业科学[J].1987,20(6):52-57
[202] Wishat.糖对新鲜的和储藏的火鸡精液受精力的影响.甘肃畜牧兽医[J].1987,(2):46
[203] 张坚中, 徐铁铮. 家畜冷冻精液 [M]. 中国农业科技出版社, 1988:27 [204] 苏东顿. 现代家禽人工授精技术.1993: 10
[205] 中国农业科学院主编. 家畜精液稀释和贮存<国外农业科学资料汇编> 第三辑. 农业出版社, 1959: 28-31
[206] 郭年藩译.家畜精液保存技术的研究. 国外畜牧科技[J].1993,1:7-8
[207] 季本林.猪精液稀释液不同用水的研究及应用.中国畜牧杂志[J].1993,(5):40-41
[208] 董金才译.家畜精液冷冻保护液.国外畜牧科技[J] 1986,(6):17-18
[209] 张兆旺. 乌骨鸡体重、冠型、与采精量关系的研究. 甘肃畜牧兽医[J]. 1994 ,6(3):9-11
[210] 张兆旺, 杨进军. 乌骨鸡精液品质测定及人工授精试验.第八届全国动繁殖学术讨会论文集.1995,23
[211] 张兆旺, 李婉平, 史兆国.公鸡体重、采精量、精子活率和密度与受精率关系的研究.第八次全国家禽学术讨论会论文集.1997,19-20
[212] 陈元明,廖纪朝,黄美玉,等.鸡精液稀释液保存技术的研究.中国农业科学[J].1987,20(6):52-57
[213] 郭年藩译. 家禽精液稀释液的发展.国外畜牧科技[J].1992,19(6):8-11