降钙素基因相关肽对大鼠运动性疲劳的影响研究
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摘要
目的：探讨CGRP对大鼠运动性疲劳的影响。
方法：成年健康雄性SD大鼠,依据动物运动至力竭的时间剔除运动能力太差和运动能力太强的大鼠依据不同的预处理措施随机分成五组：空白对照组、PBS预处理组、CGRP预处理组(降钙素基因相关肽,calcitonin gene-related peptide, CGRP)、CAP预处理组(辣椒素,capsaicin, CAP)、CAP预处理+CGRP预处理组,各组动物在安静状态时、运动80分钟时和运动至力竭后即刻处死。然后用免疫组化和放射免疫方法检测上述条件下大鼠腓肠肌神经肌肉接头CGRP的表达变化；用HE染色观察上述条件下运动性疲劳大鼠骨骼肌形态结构的变化；用化学比色的方法检测上述条件下骨骼肌超氧化物歧化酶(superoxide dismutase, SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-PX)、过氧化氢酶(catalase, CAT)及丙二醛(malondialdehyde, MDA)的变化。
结果：①给予CGRP预处理后,大鼠运动至力竭的时间明显缩短(P<0.05),而给予CAP预处理后大鼠运动至力竭的时间明显延长(P<0.05)。②CGRP免疫组织化学和放射免疫结果显示：CGRP的免疫阳性产物在肌纤维纵切面上,其形状为椭圆形或棒状,主要分布于肌纤维膜的表面。给予CGRP预处理后,大鼠腓肠肌神经肌肉接头CGRP的表达明显升高(P<0.05),而给予CAP预处理后,大鼠腓肠肌神经肌肉接头CGRP的表达明显下降(P<0.05)。③HE染色显示：安静状态下各组骨骼肌的形态结构未见明显改变；运动80min时与安静状态下相比,除CGRP预处理组骨骼肌的细胞核明显增多、增大外,其余各组均无明显改变；力竭运动后即刻与安静状态下相比除CAP预处理组无明显改变外,其余各组骨骼肌的细胞核均明显增多、增大。④化学比色结果显示：给予CGRP预处理后,大鼠骨骼肌中SOD、GSH-PX和CAT的活性明显下降(P<0.05), MDA的含量明显上升(P<0.05),而给予CAP预处理后大鼠骨骼肌中SOD、GSH-PX和CAT的活性明显上升(P<0.05),MDA的含量明显下降(P<0.05)。
小结：CGRP加剧了运动性疲劳的产生。
目的：检测CGRP对运动性疲劳大鼠AChE的影响,探讨CGRP对运动性疲劳的作用机制。
方法：实验动物分组及处理同第一部分。通过免疫组化和组织化学的方法检测不同干预措施条件下运动性疲劳大鼠腓肠肌神经肌肉接头AChE的表达情况和活性变化,并用酶联免疫吸附分析和化学比色法对AChE的表达和活性改变进行定量分析。并对大鼠腓肠肌神经肌肉接头CGRP的浓度与AChE的表达和活性进行相关分析和回归分析。
结果：①AChE免疫组织化学、酶联免疫吸附分析、组织化学和化学比色结果显示：给予CGRP预处理后,大鼠腓肠肌神经肌肉接头AChE的表达和活性显著降低(P<0.05)。而给予CAP预处理后大鼠腓肠肌神经肌肉接头AChE的表达和活性显著升高(P<0.05)。②相关分析显示大鼠腓肠肌神经肌肉接头CGRP的表达量与AChE的表达呈负性相关,其中安静状态组r=0.78(p=0.000),80分钟组r=0.92(p=0.000),力竭组r=0.88 (p=0.000);CGRP的表达量与AChE的活性也呈负性相关,其中安静状态组r=0.81(p=0.000),80分钟组r=0.92(p=0.000),力竭组r=0.89(p=0.000)。回归分析显示AChE表达的下降在安静状态组有61%(r2=0.61),在80分钟组有85%(r2=0.85),在力竭组有78%(r2=0.78)的可能是由CGRP的变化引起。AChE活性的下降在安静组有66%(r2=0.66),在80分钟组有84%(r2=0.84),在力竭组有79%(r2=0.79)的可能是由CGRP的变化引起。提示CGRP浓度的改变与大鼠腓肠肌神经肌肉接头AChE表达和活性的下降呈负相关。
小结：CGRP通过调节大鼠腓肠肌神经肌肉接头AChE的表达和活性促进了运动性疲劳的产生。
Objective To study the effects of CGRP on the exercise-induced fatigue of rats.
Methods Healthy adult male Sprague-Dawley rats were randomly divided into five groups according to the different pretreatment:the blank control group, the PBS pretreatment group, the CGRP pretreatment group, the CAP pretreatment group, the CAP pretreatment+CGRP pretreatment group. The rats were sacrificed at the resting state, the exercise to 80 min and the exercise to exhaustion, Then the immunohistochemistry and radioimmunoassay was used to detecting the expression of CGRP at the condition of the different pretreatment, the HE staining was used to detecting the change of morphous formation of skeletal muscle at the condition of the different pretreatment, the chemistry colorimetric method was used to detecting the change of superoxide dismutase, glutathione peroxidase, catalase, and malondialdehyde at the conditions of the different pretreatment.
Results①the time of the treadmill exercise to exhaustion was significantly decurtated after CGRP pretreatment(P<0.05), and the time of the treadmill exercise to exhaustion was significantly prolonged after CAP pretreatment(P<0.05).②Immunohistochemistry and radioimmunoassay showed that CGRP immunopositive products were elliptical or rod-shape at longitudinal section of muscle fiber. The expression of CGRP was significantly increased after the CGRP pretreatment(P<0.05), and the expression of CGRP was significantly decreased after the CAP pretreatment(P<0.05).③HE staining showed that there were no obvious differences in morphous constitution of skeletal muscle in all groups at the resting state. There were also no obvious changes in morphous constitution of skeletal muscle at the other groups at the exercise to 80 min than at the resting state, excluding cell nucleus of skeletal muscle were significantly increased and augmented at the CGRP pretreatment group, Cell nucleus of skeletal muscle were significantly increased and augmented in all other groups, while there were no obvious changes in morphous constitution of skeletal muscle at the CAP pretreatment group at exercise to exhaustion than at the resting state.④The chemistry colorimetric showed that the content of MDA was significantly increased(P<0.05), and the activity of SOD, GSH-PX, CAT was significantly decreased after the CGRP pretreatment(P<0.05), While the content of MDA was significantly decreased(P<0.05), and the activity of SOD, GSH-PX, CAT was significantly increased after the CAP pretreatment(P<0.05).
Conclusion The CGRP intensified the generation of exercise-induced fatigue
Objective To explore the effects of CGRP on the AChE at exercise-induced fatigued rat neuromuscular junctions.
Methods The animals and the experimental methods were the same as in the primary part. The immunohistochemistry and histochemistry was used to detecting the changes of the expression and activity of AChE. Then the expression and activity of AChE were also detected by enzyme linked immunoadsorbent assay and chemistry colorimetric method. Then linear correlation and linear regression were tested between the CGRP concentration and the expression or activity of AChE.
Results①Immunohistochemistry, enzyme linked immunoadsorbent assay, histochemistry and chemistry colorimetric method showed that the expression and activity of AChE at the gastrocnemius neuromuscular junction of rat was significantly decreased after CGRP pretreatment(P<0.05), and the expression and activity of AChE of the gastrocnemius neuromuscular junction of rat was significantly increased after the CAP pretreatment(P<0.05).②The linear correlation and regression analysis showed there was negativity correlation between the CGRP concentration and the expression AChE or and activity of AChE (resting state r=0.78; exercise to 80 min r=0.92; exercise to exhaustion r=0.88 respectively).
Conclusion Our study suggests that CGRP promoted the production of exercise-induced fatigue through the regulation the expression of AChE at the gastrocnemius neuromuscular junction of rat.
方法：成年健康雄性SD大鼠,依据动物运动至力竭的时间剔除运动能力太差和运动能力太强的大鼠依据不同的预处理措施随机分成五组：空白对照组、PBS预处理组、CGRP预处理组(降钙素基因相关肽,calcitonin gene-related peptide, CGRP)、CAP预处理组(辣椒素,capsaicin, CAP)、CAP预处理+CGRP预处理组,各组动物在安静状态时、运动80分钟时和运动至力竭后即刻处死。然后用免疫组化和放射免疫方法检测上述条件下大鼠腓肠肌神经肌肉接头CGRP的表达变化；用HE染色观察上述条件下运动性疲劳大鼠骨骼肌形态结构的变化；用化学比色的方法检测上述条件下骨骼肌超氧化物歧化酶(superoxide dismutase, SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-PX)、过氧化氢酶(catalase, CAT)及丙二醛(malondialdehyde, MDA)的变化。
结果：①给予CGRP预处理后,大鼠运动至力竭的时间明显缩短(P<0.05),而给予CAP预处理后大鼠运动至力竭的时间明显延长(P<0.05)。②CGRP免疫组织化学和放射免疫结果显示：CGRP的免疫阳性产物在肌纤维纵切面上,其形状为椭圆形或棒状,主要分布于肌纤维膜的表面。给予CGRP预处理后,大鼠腓肠肌神经肌肉接头CGRP的表达明显升高(P<0.05),而给予CAP预处理后,大鼠腓肠肌神经肌肉接头CGRP的表达明显下降(P<0.05)。③HE染色显示：安静状态下各组骨骼肌的形态结构未见明显改变；运动80min时与安静状态下相比,除CGRP预处理组骨骼肌的细胞核明显增多、增大外,其余各组均无明显改变；力竭运动后即刻与安静状态下相比除CAP预处理组无明显改变外,其余各组骨骼肌的细胞核均明显增多、增大。④化学比色结果显示：给予CGRP预处理后,大鼠骨骼肌中SOD、GSH-PX和CAT的活性明显下降(P<0.05), MDA的含量明显上升(P<0.05),而给予CAP预处理后大鼠骨骼肌中SOD、GSH-PX和CAT的活性明显上升(P<0.05),MDA的含量明显下降(P<0.05)。
小结：CGRP加剧了运动性疲劳的产生。
目的：检测CGRP对运动性疲劳大鼠AChE的影响,探讨CGRP对运动性疲劳的作用机制。
方法：实验动物分组及处理同第一部分。通过免疫组化和组织化学的方法检测不同干预措施条件下运动性疲劳大鼠腓肠肌神经肌肉接头AChE的表达情况和活性变化,并用酶联免疫吸附分析和化学比色法对AChE的表达和活性改变进行定量分析。并对大鼠腓肠肌神经肌肉接头CGRP的浓度与AChE的表达和活性进行相关分析和回归分析。
结果：①AChE免疫组织化学、酶联免疫吸附分析、组织化学和化学比色结果显示：给予CGRP预处理后,大鼠腓肠肌神经肌肉接头AChE的表达和活性显著降低(P<0.05)。而给予CAP预处理后大鼠腓肠肌神经肌肉接头AChE的表达和活性显著升高(P<0.05)。②相关分析显示大鼠腓肠肌神经肌肉接头CGRP的表达量与AChE的表达呈负性相关,其中安静状态组r=0.78(p=0.000),80分钟组r=0.92(p=0.000),力竭组r=0.88 (p=0.000);CGRP的表达量与AChE的活性也呈负性相关,其中安静状态组r=0.81(p=0.000),80分钟组r=0.92(p=0.000),力竭组r=0.89(p=0.000)。回归分析显示AChE表达的下降在安静状态组有61%(r2=0.61),在80分钟组有85%(r2=0.85),在力竭组有78%(r2=0.78)的可能是由CGRP的变化引起。AChE活性的下降在安静组有66%(r2=0.66),在80分钟组有84%(r2=0.84),在力竭组有79%(r2=0.79)的可能是由CGRP的变化引起。提示CGRP浓度的改变与大鼠腓肠肌神经肌肉接头AChE表达和活性的下降呈负相关。
小结：CGRP通过调节大鼠腓肠肌神经肌肉接头AChE的表达和活性促进了运动性疲劳的产生。
Objective To study the effects of CGRP on the exercise-induced fatigue of rats.
Methods Healthy adult male Sprague-Dawley rats were randomly divided into five groups according to the different pretreatment:the blank control group, the PBS pretreatment group, the CGRP pretreatment group, the CAP pretreatment group, the CAP pretreatment+CGRP pretreatment group. The rats were sacrificed at the resting state, the exercise to 80 min and the exercise to exhaustion, Then the immunohistochemistry and radioimmunoassay was used to detecting the expression of CGRP at the condition of the different pretreatment, the HE staining was used to detecting the change of morphous formation of skeletal muscle at the condition of the different pretreatment, the chemistry colorimetric method was used to detecting the change of superoxide dismutase, glutathione peroxidase, catalase, and malondialdehyde at the conditions of the different pretreatment.
Results①the time of the treadmill exercise to exhaustion was significantly decurtated after CGRP pretreatment(P<0.05), and the time of the treadmill exercise to exhaustion was significantly prolonged after CAP pretreatment(P<0.05).②Immunohistochemistry and radioimmunoassay showed that CGRP immunopositive products were elliptical or rod-shape at longitudinal section of muscle fiber. The expression of CGRP was significantly increased after the CGRP pretreatment(P<0.05), and the expression of CGRP was significantly decreased after the CAP pretreatment(P<0.05).③HE staining showed that there were no obvious differences in morphous constitution of skeletal muscle in all groups at the resting state. There were also no obvious changes in morphous constitution of skeletal muscle at the other groups at the exercise to 80 min than at the resting state, excluding cell nucleus of skeletal muscle were significantly increased and augmented at the CGRP pretreatment group, Cell nucleus of skeletal muscle were significantly increased and augmented in all other groups, while there were no obvious changes in morphous constitution of skeletal muscle at the CAP pretreatment group at exercise to exhaustion than at the resting state.④The chemistry colorimetric showed that the content of MDA was significantly increased(P<0.05), and the activity of SOD, GSH-PX, CAT was significantly decreased after the CGRP pretreatment(P<0.05), While the content of MDA was significantly decreased(P<0.05), and the activity of SOD, GSH-PX, CAT was significantly increased after the CAP pretreatment(P<0.05).
Conclusion The CGRP intensified the generation of exercise-induced fatigue
Objective To explore the effects of CGRP on the AChE at exercise-induced fatigued rat neuromuscular junctions.
Methods The animals and the experimental methods were the same as in the primary part. The immunohistochemistry and histochemistry was used to detecting the changes of the expression and activity of AChE. Then the expression and activity of AChE were also detected by enzyme linked immunoadsorbent assay and chemistry colorimetric method. Then linear correlation and linear regression were tested between the CGRP concentration and the expression or activity of AChE.
Results①Immunohistochemistry, enzyme linked immunoadsorbent assay, histochemistry and chemistry colorimetric method showed that the expression and activity of AChE at the gastrocnemius neuromuscular junction of rat was significantly decreased after CGRP pretreatment(P<0.05), and the expression and activity of AChE of the gastrocnemius neuromuscular junction of rat was significantly increased after the CAP pretreatment(P<0.05).②The linear correlation and regression analysis showed there was negativity correlation between the CGRP concentration and the expression AChE or and activity of AChE (resting state r=0.78; exercise to 80 min r=0.92; exercise to exhaustion r=0.88 respectively).
Conclusion Our study suggests that CGRP promoted the production of exercise-induced fatigue through the regulation the expression of AChE at the gastrocnemius neuromuscular junction of rat.
引文
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