蒙药阿给炭止血作用及其机制研究
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摘要
[目的]
研究蒙药阿给炭的止血作用及其机制,旨在为阿给炭的临床应用提供实验依据。
[方法]
1.通过小鼠断尾出血实验及玻璃毛细管凝血实验,观察阿给炭及其不同极性部位对小鼠出血时间(bleeding time, BT)、凝血时间(clotting time, CT)以及对肝素抗凝作用的影响,揭示阿给炭止血作用有效活性部位。
2.通过吲哚美辛(消炎痛)加无水乙醇法建立实验性小鼠胃溃疡出血模型。预防给药后,观察小鼠胃溃疡指数的变化,分析阿给炭对小鼠胃溃疡出血的影响。
3.通过检测大鼠给药前后凝血酶原时间(prothrombin time, PT)、活化部分凝血酶时间(activated partial thormboplastia time, APTT)、凝血酶时间(thrombin time, TT)和纤维蛋白原含量(fibrinogen, FIB)的变化,分析阿给炭对大鼠凝血系统功能的影响。
4.通过流式细胞术检测大鼠给药前后血小板表面膜糖蛋白CD61平均荧光强度(MFI)和CD62P阳性百分率的变化,分析阿给炭对大鼠血小板活化的影响；结合血小板数目和形态的变化,分析其对大鼠血小板系统功能的影响。
5.通过检测给药前后家兔血液流变学指标的变化,分析阿给炭对血液流变状态及对血栓形成的影响。
[结果]
1.阿给炭能显著缩短正常小鼠和肝素化小鼠的BT和CT(P<0.01),水部位和正丁醇部位能缩短正常小鼠BT和CT(P<0.01或P<0.05),乙酸乙酯部位对正常小鼠BT和CT无影响(P>0.05)；正丁醇部位、水部位和乙酸乙酯部位均能缩短肝素化小鼠BT和CT(P<0.01或P<0.05)。
2.阿给炭低、中、高剂量组均能显著降低小鼠胃溃疡出血指数(P<0.01)。
3.阿给炭中、高剂量组能缩短大鼠PT(P<0.01或P<0.05)；高剂量组能缩短大鼠APTT(P<0.05);低、中、高剂量组对大鼠TT和FIB均无影响(P>0.05)。
4.阿给炭高剂量组能显著增加大鼠血小板CD61平均荧光强度和CD62P阳性百分率(P<0.01)。
5.阿给炭低、高剂量组均能显著增加大鼠血小板数目(P<0.01),低、中、高剂量组均能显著缩小家兔血小板分布宽度(P<0.01),对平均血小板体积均无影响(P>0.05)。
6.阿给炭低剂量组能增加家兔红细胞变形指数(P<0.05),中剂量组能减少家兔血沉(P<0.05),高剂量组能缩小家兔平均红细胞体积(P<0.05)。低、中、高剂量组对家兔不同切变率下全血黏度、血浆黏度、全血还原黏度、红细胞压积、红细胞聚集指数和刚性指数均无影响(P>0.05)。
[结论]
1.阿给炭及其水部位和正丁醇部位均有止血作用,但从作用趋势来看,阿给炭的止血效果比水部位、正丁醇部位更好。
2.阿给炭对胃溃疡出血具有防治作用。
3.阿给炭通过影响内、外源性凝血系统发挥止血作用。
4.阿给炭通过增加血小板数目和活化血小板功能发挥止血作用。
5.阿给炭通过减慢血沉、增加红细胞变形指数,可以降低血管的外周阻力,部分抑制血栓形成。
Objectives:
We study the hemostatic effects of carbonized Agei and explore the hemostatic mechanism. These provide experimental basis for its clinical application.
Methods:
1. The mouse bleeding time was measured by severing tails test, and the clotting time was measured by capillary test.
2. The experimental model of gastric ulcer was established by Indomethacin and absolute ethyl alcohol. Observing the changing of gastric ulcer exponential after prophylactic, we analyze the effects of carbonized Agei on the mouse gastric ulcer exponential.
3. The PT, APTT, TT and FIB were measured for analyze the effects of carbonized Agei on coagulation system.
4. The MFI of CD61 and the positive rate of CD62P were measured by Flow Cytometry. We analyze the effects of carbonized Agei on platelet activation system. Combined with the changing of platelet count and morphology, we ananlyze the effects of carbonized Agei on platelet system.
5. The indexes of hemorrheology were measured by blood rheometer. We analyze the risk of carbonized Agei on thrombosis.
Results:
1. We obtain effects of different extract from carbonized Agei on the mouse BT and CT. In the group with carbonized Agei, BT and CT were significantly declined (P<0.01). The water part and n-butanol extracted from carbonized Agei can significantly shorten mouse BT and CT (P<0.01). The ethyl acetate extracted from carbonized Agei had no effect on mouse BT and CT (P>0.05)
We obtain effects of different extract from carbonized Agei on the heparinized mouse BT and CT. In the group with carbonized Agei, BT and CT were significantly declined (P<0.01). The water part, n-butanol and ethyl acetate extracted from carbonized Agei can shorten heparinized BT and CT (P<0.01或P<0.05)
2. The low dose group, middle dose group and high dose group of carbonized Agei can significantly shorten mouse gastric ulcer exponential (P<0.01).
3. The middle dose group and high dose group of carbonized Agei can shorten mouse PT (P<0.01或P<0.05). The high dose group of carbonized Agei can shorten mouse APTT(P<0.05). The low dose group, middle dose group and high dose group of carbonized Agei had no effect on mouse TT and FIB (P>0.05)
4. The high dose group of carbonized Agei can significantly increase the MFI of CD61and the positive rate of CD62P (P<0.01)
5. The middle dose group and high dose group of carbonized Agei can significantly increase the platelet count(P<0.01). The low dose group, middle dose group and high dose group of carbonized Agei can significantly shorten platelet distribution width (P<0.01). It had no effect on the mean corpuscular volume (P>0.05)
6. The low dose group of carbonized Agei can increase the erythrocyte deformability of red cell (P<0.05). The middle dose group of carbonized Agei can decrease blood sedimentation (P<0.05). The high dose group of carbonized Agei can shortenr cell morphology (P<0.05) The low dose group, middle dose group and high dose group of carbonized Agei had no effect on the whole blood viscosity of rabbit in different shear rate, plasma viscosity, blood reduction viscosity, the gathering index, erythrocyte index of rigidity of red cell (P>0.05)
Conclusions:
1. The carbonized Agei, the water part and n-butanol extracted from carbonized Agei have the favorable effects on haemostasis and coagulation, but carbonized Agei as a whole has better effect according to its developing trend.
2. Carbonized Agei has favorable effects on preventing and curing the experimental gastric ulcer model.
3. Carbonized Agei can perform function of hemostasis through intrinsic and extrinsic coagulation pathway.
4. Carbonized Agei can perform function of hemostasis through increased platelet count and platelet activation.
5. Carbonized Agei can reduce the peripheral resistance of blood vessel and inhibit thrombosis to a certain degree through decrease blood sedimentation and increase the erythrocyte deformability of Red Cells.
研究蒙药阿给炭的止血作用及其机制,旨在为阿给炭的临床应用提供实验依据。
[方法]
1.通过小鼠断尾出血实验及玻璃毛细管凝血实验,观察阿给炭及其不同极性部位对小鼠出血时间(bleeding time, BT)、凝血时间(clotting time, CT)以及对肝素抗凝作用的影响,揭示阿给炭止血作用有效活性部位。
2.通过吲哚美辛(消炎痛)加无水乙醇法建立实验性小鼠胃溃疡出血模型。预防给药后,观察小鼠胃溃疡指数的变化,分析阿给炭对小鼠胃溃疡出血的影响。
3.通过检测大鼠给药前后凝血酶原时间(prothrombin time, PT)、活化部分凝血酶时间(activated partial thormboplastia time, APTT)、凝血酶时间(thrombin time, TT)和纤维蛋白原含量(fibrinogen, FIB)的变化,分析阿给炭对大鼠凝血系统功能的影响。
4.通过流式细胞术检测大鼠给药前后血小板表面膜糖蛋白CD61平均荧光强度(MFI)和CD62P阳性百分率的变化,分析阿给炭对大鼠血小板活化的影响；结合血小板数目和形态的变化,分析其对大鼠血小板系统功能的影响。
5.通过检测给药前后家兔血液流变学指标的变化,分析阿给炭对血液流变状态及对血栓形成的影响。
[结果]
1.阿给炭能显著缩短正常小鼠和肝素化小鼠的BT和CT(P<0.01),水部位和正丁醇部位能缩短正常小鼠BT和CT(P<0.01或P<0.05),乙酸乙酯部位对正常小鼠BT和CT无影响(P>0.05)；正丁醇部位、水部位和乙酸乙酯部位均能缩短肝素化小鼠BT和CT(P<0.01或P<0.05)。
2.阿给炭低、中、高剂量组均能显著降低小鼠胃溃疡出血指数(P<0.01)。
3.阿给炭中、高剂量组能缩短大鼠PT(P<0.01或P<0.05)；高剂量组能缩短大鼠APTT(P<0.05);低、中、高剂量组对大鼠TT和FIB均无影响(P>0.05)。
4.阿给炭高剂量组能显著增加大鼠血小板CD61平均荧光强度和CD62P阳性百分率(P<0.01)。
5.阿给炭低、高剂量组均能显著增加大鼠血小板数目(P<0.01),低、中、高剂量组均能显著缩小家兔血小板分布宽度(P<0.01),对平均血小板体积均无影响(P>0.05)。
6.阿给炭低剂量组能增加家兔红细胞变形指数(P<0.05),中剂量组能减少家兔血沉(P<0.05),高剂量组能缩小家兔平均红细胞体积(P<0.05)。低、中、高剂量组对家兔不同切变率下全血黏度、血浆黏度、全血还原黏度、红细胞压积、红细胞聚集指数和刚性指数均无影响(P>0.05)。
[结论]
1.阿给炭及其水部位和正丁醇部位均有止血作用,但从作用趋势来看,阿给炭的止血效果比水部位、正丁醇部位更好。
2.阿给炭对胃溃疡出血具有防治作用。
3.阿给炭通过影响内、外源性凝血系统发挥止血作用。
4.阿给炭通过增加血小板数目和活化血小板功能发挥止血作用。
5.阿给炭通过减慢血沉、增加红细胞变形指数,可以降低血管的外周阻力,部分抑制血栓形成。
Objectives:
We study the hemostatic effects of carbonized Agei and explore the hemostatic mechanism. These provide experimental basis for its clinical application.
Methods:
1. The mouse bleeding time was measured by severing tails test, and the clotting time was measured by capillary test.
2. The experimental model of gastric ulcer was established by Indomethacin and absolute ethyl alcohol. Observing the changing of gastric ulcer exponential after prophylactic, we analyze the effects of carbonized Agei on the mouse gastric ulcer exponential.
3. The PT, APTT, TT and FIB were measured for analyze the effects of carbonized Agei on coagulation system.
4. The MFI of CD61 and the positive rate of CD62P were measured by Flow Cytometry. We analyze the effects of carbonized Agei on platelet activation system. Combined with the changing of platelet count and morphology, we ananlyze the effects of carbonized Agei on platelet system.
5. The indexes of hemorrheology were measured by blood rheometer. We analyze the risk of carbonized Agei on thrombosis.
Results:
1. We obtain effects of different extract from carbonized Agei on the mouse BT and CT. In the group with carbonized Agei, BT and CT were significantly declined (P<0.01). The water part and n-butanol extracted from carbonized Agei can significantly shorten mouse BT and CT (P<0.01). The ethyl acetate extracted from carbonized Agei had no effect on mouse BT and CT (P>0.05)
We obtain effects of different extract from carbonized Agei on the heparinized mouse BT and CT. In the group with carbonized Agei, BT and CT were significantly declined (P<0.01). The water part, n-butanol and ethyl acetate extracted from carbonized Agei can shorten heparinized BT and CT (P<0.01或P<0.05)
2. The low dose group, middle dose group and high dose group of carbonized Agei can significantly shorten mouse gastric ulcer exponential (P<0.01).
3. The middle dose group and high dose group of carbonized Agei can shorten mouse PT (P<0.01或P<0.05). The high dose group of carbonized Agei can shorten mouse APTT(P<0.05). The low dose group, middle dose group and high dose group of carbonized Agei had no effect on mouse TT and FIB (P>0.05)
4. The high dose group of carbonized Agei can significantly increase the MFI of CD61and the positive rate of CD62P (P<0.01)
5. The middle dose group and high dose group of carbonized Agei can significantly increase the platelet count(P<0.01). The low dose group, middle dose group and high dose group of carbonized Agei can significantly shorten platelet distribution width (P<0.01). It had no effect on the mean corpuscular volume (P>0.05)
6. The low dose group of carbonized Agei can increase the erythrocyte deformability of red cell (P<0.05). The middle dose group of carbonized Agei can decrease blood sedimentation (P<0.05). The high dose group of carbonized Agei can shortenr cell morphology (P<0.05) The low dose group, middle dose group and high dose group of carbonized Agei had no effect on the whole blood viscosity of rabbit in different shear rate, plasma viscosity, blood reduction viscosity, the gathering index, erythrocyte index of rigidity of red cell (P>0.05)
Conclusions:
1. The carbonized Agei, the water part and n-butanol extracted from carbonized Agei have the favorable effects on haemostasis and coagulation, but carbonized Agei as a whole has better effect according to its developing trend.
2. Carbonized Agei has favorable effects on preventing and curing the experimental gastric ulcer model.
3. Carbonized Agei can perform function of hemostasis through intrinsic and extrinsic coagulation pathway.
4. Carbonized Agei can perform function of hemostasis through increased platelet count and platelet activation.
5. Carbonized Agei can reduce the peripheral resistance of blood vessel and inhibit thrombosis to a certain degree through decrease blood sedimentation and increase the erythrocyte deformability of Red Cells.
引文
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