三七总皂苷治疗大鼠SAH后CVS影响的实验研究
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摘要
目的：研究三七总皂苷对大鼠SAH后CVS的影响及其对大鼠脑血管管壁中TNF-α的表达的改变,从而验证三七总皂苷能否通过抑制大鼠脑血管管壁中TNF-α的表达来有效地预防或减轻蛛网膜下腔出血所导致的CVS引起的脑损害。
方法：将SD大鼠随机分为十组：假手术组(3d、7d),生理盐水组(3d、7d),SAH组(3d、7d),三七总皂苷干预的SAH组(PNS治疗组3d、7d),三七总皂苷预处理的SAH组(PNS预处理组3d、7d)。手术建模,建模后3d,7d进行动物神经行为学评分,并取出动物基底动脉脑桥段,切片后观察血管的痉挛情况及血管壁的形态改变,并用免疫组化法测定血管壁中炎症指标TNF-α的表达的改变,结果进行统计学处理。
结果：PNS治疗组及PNS预处理组3d及7d时的动物神经行为评分与SAH组3d及7d时比较均明显降低(P<0.05),7d时PNS治疗组及PNS预处理组的基底动脉管壁厚度与SAH组比较差异亦有明显变薄(P<0.05)。7d时SAH组TNF-α的表达最强,PNS治疗组及PNS预处理组其次,对照组及假手术组有微弱表达。PNS治疗组及预处理组同一时相之间评分、基底动脉管壁厚度、TNF-α表达比较差异均无明显差异(P>0.05)。
结论：1.血管壁的局部炎症反应可能是蛛网膜下腔出血后引起的慢性血管痉挛的主要原因,其中TNF-α在血管壁的局部炎症反应中起了重要的作用。
2.三七总皂苷能有效的降低蛛网膜下腔出血后引起的慢性血管痉挛的严重程度。
3.三七总皂苷防治慢性血管痉挛的机理可能是通过抑制TNF-α的表达而发挥作用。
4.预先使用三七总皂苷及SHA后使用三七总皂苷治疗之间比较,效果无明显差异。
Objective:To study the PNS'S effects of CVS after SAH in rats and its impact on the expression of TNF-αchanges in the cerebrovascular wall, in order to verify whether PNS could effectively prevent or mitigate the brain damage caused by CVS after SAH by inhibiting the expression of TNF-αin the cerebrovascular in rats。
Methods:Randomly divide the SD rats into ten groups: sham-operated group (3-days、7-days), saline group (3-days、7-days), SAH group(3-days、7-days), SAH with PNS intervention group(3-days、7-days) and PNS pretreatment before SAH group (3-days、7-days) Surgeries were operated for modeling. Then we scored on animal neurological behaviour at 3rd day,7th day after modeling, removed brain pontine basilar artery to observe the sections of blood vessels spasming and morphology changes of cerebrovascular walls in biopsy, and used immunohistochemical method to identify the expression changes of vascular wall inflammation indicators TNF-α. The data was processed by Statistics finally。
Results:There was significant statistical difference(P<0.05)between PNS treatment group/PNS pretreatment group and SAH group at the time of 3rd day and 7th day on animals neurobehavior score, and the same significant statistical difference (P<0.05) on basilar artery wall thickness。 The SAH group showed the strongest TNF-αexpression at the 7th day, PNS treatment group and PNS pretreatment group were the second strongest. The sham-operated group and saline group had no significant expression. There were no statistical significance difference(P>0.05) between PNS treatment group and PNS pretreatment group on animals neurobehavior score, basilar artery wall thickness and expression of TNF-α.
Conclusion:1. The local inflammatory response on cerebrovascular wall may be the main reason caused by CVS after SAH, in which TNF-αplays an important role in the local inflammatory response of the cerebrovascular wall.
2. PNS can effectively reduce the severity of CVS after SAH.
3. The mechanism of PNS preventing and treating the CVS may be by inhibiting the expression of TNF-α.
4. There is no significant difference between the effects of PNS pretreatment and PNS treatment
方法：将SD大鼠随机分为十组：假手术组(3d、7d),生理盐水组(3d、7d),SAH组(3d、7d),三七总皂苷干预的SAH组(PNS治疗组3d、7d),三七总皂苷预处理的SAH组(PNS预处理组3d、7d)。手术建模,建模后3d,7d进行动物神经行为学评分,并取出动物基底动脉脑桥段,切片后观察血管的痉挛情况及血管壁的形态改变,并用免疫组化法测定血管壁中炎症指标TNF-α的表达的改变,结果进行统计学处理。
结果：PNS治疗组及PNS预处理组3d及7d时的动物神经行为评分与SAH组3d及7d时比较均明显降低(P<0.05),7d时PNS治疗组及PNS预处理组的基底动脉管壁厚度与SAH组比较差异亦有明显变薄(P<0.05)。7d时SAH组TNF-α的表达最强,PNS治疗组及PNS预处理组其次,对照组及假手术组有微弱表达。PNS治疗组及预处理组同一时相之间评分、基底动脉管壁厚度、TNF-α表达比较差异均无明显差异(P>0.05)。
结论：1.血管壁的局部炎症反应可能是蛛网膜下腔出血后引起的慢性血管痉挛的主要原因,其中TNF-α在血管壁的局部炎症反应中起了重要的作用。
2.三七总皂苷能有效的降低蛛网膜下腔出血后引起的慢性血管痉挛的严重程度。
3.三七总皂苷防治慢性血管痉挛的机理可能是通过抑制TNF-α的表达而发挥作用。
4.预先使用三七总皂苷及SHA后使用三七总皂苷治疗之间比较,效果无明显差异。
Objective:To study the PNS'S effects of CVS after SAH in rats and its impact on the expression of TNF-αchanges in the cerebrovascular wall, in order to verify whether PNS could effectively prevent or mitigate the brain damage caused by CVS after SAH by inhibiting the expression of TNF-αin the cerebrovascular in rats。
Methods:Randomly divide the SD rats into ten groups: sham-operated group (3-days、7-days), saline group (3-days、7-days), SAH group(3-days、7-days), SAH with PNS intervention group(3-days、7-days) and PNS pretreatment before SAH group (3-days、7-days) Surgeries were operated for modeling. Then we scored on animal neurological behaviour at 3rd day,7th day after modeling, removed brain pontine basilar artery to observe the sections of blood vessels spasming and morphology changes of cerebrovascular walls in biopsy, and used immunohistochemical method to identify the expression changes of vascular wall inflammation indicators TNF-α. The data was processed by Statistics finally。
Results:There was significant statistical difference(P<0.05)between PNS treatment group/PNS pretreatment group and SAH group at the time of 3rd day and 7th day on animals neurobehavior score, and the same significant statistical difference (P<0.05) on basilar artery wall thickness。 The SAH group showed the strongest TNF-αexpression at the 7th day, PNS treatment group and PNS pretreatment group were the second strongest. The sham-operated group and saline group had no significant expression. There were no statistical significance difference(P>0.05) between PNS treatment group and PNS pretreatment group on animals neurobehavior score, basilar artery wall thickness and expression of TNF-α.
Conclusion:1. The local inflammatory response on cerebrovascular wall may be the main reason caused by CVS after SAH, in which TNF-αplays an important role in the local inflammatory response of the cerebrovascular wall.
2. PNS can effectively reduce the severity of CVS after SAH.
3. The mechanism of PNS preventing and treating the CVS may be by inhibiting the expression of TNF-α.
4. There is no significant difference between the effects of PNS pretreatment and PNS treatment
引文
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