宫环止血汤对宫环出血肝郁血瘀证患者血清蛋白质表达的影响
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摘要
目的：从血清蛋白质表达水平探讨宫环止血汤治疗宫环出血肝郁血瘀证的机理。
方法：追踪采集宫环出血妇女置器前后和宫环止血片治疗前后血清,并采集置器后正常妇女的血清,确定95份有效血清,共分为五组,A组(置环前血清样本,置环后月经基本正常)22份,B组(置环前血清样本,置环后月经异常)9份,C组(置环后血清样本,置环后月经基本正常)13份,D组(置环后血清样本,置环后月经异常,肝郁血瘀证)41份,E组(对肝郁血瘀证宫环出血患者进行治疗后的血清样本)10份。采用蛋白质芯片-飞行质谱技术进行检测,得到血清蛋白质图谱。用Biomarker Wizard软件比较各血清蛋白质图谱,从宫环止血片治疗前后血清蛋白质表达水平上的变化,找出与治疗相关的蛋白质,结合生物信息,探讨其作用机理。结果：通过宫环止血汤治疗后,许多异常表达的血清蛋白含量得到了纠正,恢复了正常,质核比为22876.8和23480.9的二种血清蛋白与宫环止血汤的治疗机理有较大关联。
结论：通过检索蛋白质数据库,找出Lin7b和Rab25可能与宫环止血汤的治疗机理有关,为下一步研究指明了方向。
Objective:To deal with the treatment mechanism of Gonghuanzhixue soup treating metrorrhagia after women wearing IUD of liver depression and blood stasis syndrome from expression level of serum protein.
Methods:Collecting abnormal metrorrhagia women's serum of before and after inserting IUD,and before and after treatment using Gonghuanzhixue therapeutic,and the normal women's serum after wearing IUD determining the effective serum 95 were divided into five groups, A group(serum samples pre-set IUD,normal menstruation after the set IUD)22,B group (serum samples pre-set IUD,abnormal menstruation after the set IUD)9,C group(serum samples after the set IUD,normal menstruation after the set IUD)13,D group(serum samples after the set IUD, abnormal menstruation after the set IUD,liver depression and blood stasis syndrome)41,E group(serum samples before and after treating metrorrhagia after women wearing IUD of liver depression and blood stasis syndrome) 10.Using SELDI-TOF-MS serum protein mass spectrums were obtained. Compared with the Biomarker Wizard software detecting serum protein mass spectrums, from Gonghuanzhixue soup before and after treatment of serum protein expression level changes,treatment-related proteins were identified, combined with bioinformatics,to explore its mechanism.
Results:Through the treatment Gonghuanzhixue soup, many abnormal expression of the serum protein levels have been corrected and restored to normal.It was founded that charge-to-mass ratio were 22876.8 and 23480.9 were related to the treatment mechanism of Gonghuanzhixue soup.
Conclusion:From retrieving the protein databases,Lin7b and Rab25 were possible related to the treatment mechanism of Gonghuanzhixue soup. Pointed out the direction for further research.
方法：追踪采集宫环出血妇女置器前后和宫环止血片治疗前后血清,并采集置器后正常妇女的血清,确定95份有效血清,共分为五组,A组(置环前血清样本,置环后月经基本正常)22份,B组(置环前血清样本,置环后月经异常)9份,C组(置环后血清样本,置环后月经基本正常)13份,D组(置环后血清样本,置环后月经异常,肝郁血瘀证)41份,E组(对肝郁血瘀证宫环出血患者进行治疗后的血清样本)10份。采用蛋白质芯片-飞行质谱技术进行检测,得到血清蛋白质图谱。用Biomarker Wizard软件比较各血清蛋白质图谱,从宫环止血片治疗前后血清蛋白质表达水平上的变化,找出与治疗相关的蛋白质,结合生物信息,探讨其作用机理。结果：通过宫环止血汤治疗后,许多异常表达的血清蛋白含量得到了纠正,恢复了正常,质核比为22876.8和23480.9的二种血清蛋白与宫环止血汤的治疗机理有较大关联。
结论：通过检索蛋白质数据库,找出Lin7b和Rab25可能与宫环止血汤的治疗机理有关,为下一步研究指明了方向。
Objective:To deal with the treatment mechanism of Gonghuanzhixue soup treating metrorrhagia after women wearing IUD of liver depression and blood stasis syndrome from expression level of serum protein.
Methods:Collecting abnormal metrorrhagia women's serum of before and after inserting IUD,and before and after treatment using Gonghuanzhixue therapeutic,and the normal women's serum after wearing IUD determining the effective serum 95 were divided into five groups, A group(serum samples pre-set IUD,normal menstruation after the set IUD)22,B group (serum samples pre-set IUD,abnormal menstruation after the set IUD)9,C group(serum samples after the set IUD,normal menstruation after the set IUD)13,D group(serum samples after the set IUD, abnormal menstruation after the set IUD,liver depression and blood stasis syndrome)41,E group(serum samples before and after treating metrorrhagia after women wearing IUD of liver depression and blood stasis syndrome) 10.Using SELDI-TOF-MS serum protein mass spectrums were obtained. Compared with the Biomarker Wizard software detecting serum protein mass spectrums, from Gonghuanzhixue soup before and after treatment of serum protein expression level changes,treatment-related proteins were identified, combined with bioinformatics,to explore its mechanism.
Results:Through the treatment Gonghuanzhixue soup, many abnormal expression of the serum protein levels have been corrected and restored to normal.It was founded that charge-to-mass ratio were 22876.8 and 23480.9 were related to the treatment mechanism of Gonghuanzhixue soup.
Conclusion:From retrieving the protein databases,Lin7b and Rab25 were possible related to the treatment mechanism of Gonghuanzhixue soup. Pointed out the direction for further research.
引文
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[1]张新春,雷磊.宫环出血中西医结合实验研究进展[J].中医药导报,2007,1,13(1)：85-91
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[5]文乐兮,雷磊,付灵梅等.宫环止血片抗炎作用的研究[J].江西中医学院学报2003,12,15(4)：45-47
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[9]雷磊,贺林,付灵梅等.宫环止血片对离体大鼠子宫平滑肌收缩活动的影响[J].中国中医药信息杂志,2004,6,11(6)：495-497
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[12]文乐兮,雷磊,尤昭玲,等.宫环止血片对置铜宫内节育器家兔子宫内膜形态学的影响[J].湖南中医学院学报，2002,22(1)：17-21
[13]付灵梅,尤昭玲,雷磊等.宫环止血片止血及镇痛作用的实验研究[J].中西医结合学报,2004,3,2(2)：129-131
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[2]尤昭玲.中西医结合妇产科学[M].北京：中国中医药出版社,2001,434-436.
[3]周春香.中医药治疗放置宫内节育器后月经异常的疗效观察[J].中医杂志,1995,(5)：296
[4]丁丽仙,丁启后.“从瘀、热论治”上环术后诸症68例[J].贵阳中医学院学报.1994,(3)：22.
[5]王秋凤,陈朝峰.黑蒲黄汤治疗宫环出血60例[J].光明中医,2009,24(7)：1287-1288.
[6]张青云.宫环汤防治宫环出血40例临床观察[J].湖南中医药导报,2003,9(3)：27-28.
[10]Toppozada M,et.Treatment Of increased menstyuai blood loss in IUD users[J].Contracetion,1987,36(1):145-57.
[11]Pan jf,yu yi,wang lj et al.The morphologic changes of endometrial spiral arterioles in IUD induced menorrhagia[J].Adv Contracept,1994,10:213-22.
[12]Wu T,Buck G.Side effects and discontinuation rates Of in trauterine Contraceptive ddevices use in the jilin province Of China[J].Asia PacJ public health,1998,0(1):33-38.
[10]许丽绵,欧阳惠卿.宫内节育器引起子宫异常出血的机理研究和中医治疗进展[J].新中医.2001,23(9)：74-75
[11]陈冬梅.宫内节育器引起子宫异常出血的机理和中西医治疗进展[J].广西中医学院学报2004,7(2)：66-68
[12]刘艳,温美珍.独一味治疗上环后出血100例临床观察[J].山西妇幼卫生,2001,12(1)：55
[13]徐莉,杨洁,谢波.妇宁颗粒治疗宫环出血80例临床报道[J].国际医药卫生导报,2007,13,(10)：93-95.
[14]高月平.安环止血汤治疗宫环出血临床研究[J].实用中医药杂志.2008,24(12)：755-756
[15]王燕芬.血竭安宫汤治疗上环后月经失调68例[J].浙江中医杂志,2000,3(8)：335.
[16]余军.祛瘀调冲汤治疗宫环出血50例临床观察[M].中国民间疗法,2008,16(4)：22.
[17]李大剑.经验方桃红二丹四物汤临症举隅[J].安徽中医临床杂志,1998,10(5)：303-304
[18]刘玉兰,王忠轩,刘凤娟.自拟安宫环汤治疗宫环出血30例临床观察[J].中医药信息,2009,26(5)：96-97.
[19]王艳,许燕萍,杜凤娟.固本止崩汤加减治疗上环后出血100例[J].河南中医,2007,27(5)：55-56.
[20]王净.逐瘀止血汤加减治疗带环后月经过多52例[J].湖北中医杂志,1992,14(6)：23.
[21]郭荷先,潘乐意,张菊新.桃红四物汤加减治疗宫环出血300例[J].国医论坛,2005,20(3)：33.
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